CN217230709U - Cell filter - Google Patents

Abstract

The invention relates to a cell filter, which comprises a gun head body, a filter membrane column and a sample introduction pipeline; the upper end of the gun head body is provided with a connecting part detachably connected with the pipette gun, an accommodating cavity is formed between the lower end of the gun head body and the lower end of the connecting part, a sample introduction pipeline is arranged in the accommodating cavity, and the top end of the sample introduction pipeline is provided with a first valve; the periphery of introduction pipeline is provided with the filter membrane post, the both ends and the rifle head body of filter membrane post are connected relatively, form the recovery passageway between the outer wall of filter membrane post and the inner wall that holds the chamber, the bottom department of recovery passageway is provided with out the appearance mouth, this cell filter cooperation pipetting gun's use has simplified the filterable operation process of cell, and is simple to use, adopts the filter membrane post to filter the cell simultaneously, has avoided impaired among the cell filtration process to cell filtration's efficiency has been improved to a certain extent.

Description

Cell filter

Technical Field

The present disclosure relates to the field of cell filtration technology, and in particular, to a cell filter.

Background

In the experiments of flow cytometry, single cell sequencing and the like, a cell sample needs to be filtered before being installed, so that the channel is prevented from being blocked by impurities.

The existing cell filtration method comprises a labeling method and a non-labeling method, wherein the labeling method refers to labeling on a cell antibody, and a target cell is screened by utilizing the characteristic of specific combination of an antigen and the antibody through a technology; the non-labeling method is to classify cells according to their physical properties, such as cell size, motion, hydrophilicity and hydrophobicity, and electricity, while the existing cell filtration method is complex in operation, low in efficiency, and can damage cells to a certain extent.

SUMMERY OF THE UTILITY MODEL

To solve the above technical problem or at least partially solve the above technical problem, the present disclosure provides a cell filter.

The present disclosure provides a cell filter, which comprises a gun head body, a filter membrane column and a sample introduction pipeline;

the upper end of the gun head body is provided with a connecting part which is detachably connected with the pipette gun, an accommodating cavity is formed between the lower end of the gun head body and the lower end of the connecting part, the sample introduction pipeline is arranged in the accommodating cavity, and the top end of the sample introduction pipeline is provided with a first valve;

the filter membrane column is arranged on the periphery of the sample inlet pipeline, two ends of the filter membrane column are oppositely connected with the gun head body, a recovery channel is formed between the outer wall of the filter membrane column and the inner wall of the accommodating cavity, and a sample outlet is formed in the bottom end of the recovery channel.

Optionally, the outer surface of the sample introduction pipeline is provided with a liquid circulation structure.

Optionally, the outer surface of the liquid flow structure is in the shape of helical teeth.

Optionally, one end of the sample introduction pipeline close to the first valve is lower than the top end of the liquid flow structure, and the top end of the first valve is located above the top end of the liquid flow structure.

Optionally, a second valve is arranged at the sample outlet;

the first valve and the second valve are one-way valves.

Optionally, the lance head body comprises an upper lance head and a lower lance head, and the upper lance head is connected with the lower lance head oppositely;

the upper gun head is sequentially provided with the interface part and the accommodating cavity from top to bottom; the lower gun head comprises the sample introduction pipeline and the liquid circulation structure.

Optionally, the lower gun head further comprises a base, the base is arranged at the bottom end of the liquid circulation structure, and the base and the liquid circulation structure are integrally formed;

the outer wall of one end, close to the liquid circulation structure, of the base is connected with the inner wall of the accommodating cavity in an opposite mode, and one end of the filter membrane column is connected with one side, close to the liquid circulation structure, of the base in an opposite mode;

the sample introduction pipeline penetrates through the base.

Optionally, the aperture of the accommodating cavity is larger than that of the interface part, and the other end of the filter membrane column is relatively fixed to the upper surface of the accommodating cavity close to the interface part.

Optionally, the sample outlet is arranged at a connecting part of the base and the inner wall of the accommodating cavity;

the number of the sample outlets is at least one, and the second valves correspond to the sample outlets one to one.

Optionally, the outer diameters of the upper lance head and the base at a position below the connecting portion from top to bottom are gradually reduced.

Compared with the prior art, the technical scheme provided by the embodiment of the disclosure has the following advantages:

the cell filter provided by the disclosure comprises a gun head body, a filter membrane column and a sample injection pipeline; the upper end of the gun head body is provided with a connecting part detachably connected with the pipette gun, an accommodating cavity is formed between the lower end of the gun head body and the lower end of the connecting part, a sample introduction pipeline is arranged in the accommodating cavity, and the top end of the sample introduction pipeline is provided with a first valve; the periphery of introduction pipeline is provided with the filter membrane post, and the both ends and the rifle head body of filter membrane post are connected relatively, form the recovery passageway between the outer wall of filter membrane post and the inner wall that holds the chamber, and the bottom department of recovery passageway is provided with out the appearance mouth. The arrangement is that when the cell suspension to be filtered is filtered, the gun head body is detachably connected with the pipette gun, the gun head body is convenient to assemble and connect with the pipette gun, meanwhile, the gun head body is convenient to replace, the cell suspension to be filtered is sucked into the accommodating cavity through the suction of the pipette gun and flows into the space between the sample introduction pipeline and the filter membrane column through the sample introduction pipeline and the first valve, the filter membrane column filters the cell suspension to be filtered, the filtered cell sample enters the recovery channel and flows out of the sample outlet, the cell filter is matched with the pipette gun, the cell filtering operation process is simplified, the use is simple, the filter membrane column is adopted to filter cells simultaneously, the damage in the cell filtering process is avoided, and the cell filtering efficiency is improved to a certain extent.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and, together with the description, serve to explain the principles of the disclosure.

In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present disclosure, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without inventive exercise.

Fig. 1 is a schematic cross-sectional view of a cell filter taken along a sample outlet according to an embodiment of the disclosure.

Wherein, 1, a gun head body; 2. feeding a gun head; 3. a membrane filtration column; 4. a liquid flow-through structure; 5. a recovery channel; 6. feeding a gun head; 7. a sample outlet; 8. a second valve; 9. a base; 10. a sample introduction pipeline; 11. a sample inlet; 12. an interface section; 13. an accommodating chamber; 14. a first valve.

Detailed Description

In order that the above objects, features and advantages of the present disclosure may be more clearly understood, aspects of the present disclosure will be further described below. It should be noted that the embodiments and features of the embodiments of the present disclosure may be combined with each other without conflict.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present disclosure, but the present disclosure may be practiced in other ways than those described herein; it is to be understood that the embodiments disclosed in the specification are only a few embodiments of the present disclosure, and not all embodiments.

Referring to fig. 1, the present embodiment provides a cell filter, which specifically includes a gun head body 1, a filter membrane column 3, and a sample injection pipeline 10.

The upper end of the gun head body 1 is provided with the interface part 12 which is detachably connected with the pipette gun, the gun head body 1 and the pipette gun which are detachably connected are convenient for disassembling and assembling the gun head body 1 and the pipette gun, and the gun head body 1 is also convenient for replacing, so that other filtering equipment is not needed in the process of filtering cell suspension to be filtered, thereby shortening the cell filtering operation time and reducing the cell filtering cost. It can be understood that the interface part 12 of the pipette tip body 1 and the pipette tip body can be connected by screw threads, that is, an internal thread is provided on the inner wall of the interface part 12, and an external thread matched with the internal thread is provided on the pipette tip at the position connected with the interface part 12; certainly, the pipette may also be connected to the interface portion 12 of the pipette head body 1 by a snap-fit manner, that is, the interface portion 12 is provided with a snap-fit, a position on the pipette head connected to the interface portion 12 is provided with a chuck matched with the snap-fit to detach the pipette head body 1 from the pipette head body, a containing cavity 13 is formed between the lower end of the pipette head body 1 and the lower end of the interface portion 12, the sample introduction pipeline 10 is arranged in the containing cavity 13, and the top end of the sample introduction pipeline 10 is provided with the first valve 14.

When the flexible adjustable range gun head is used specifically, the measuring range and the size of the gun head body 1 can be flexibly designed according to different filtering volumes of cell suspensions to be filtered.

The periphery of the sample introduction pipeline 10 is provided with a filter membrane column 3, two ends of the filter membrane column 3 are oppositely connected with the gun head body 1, a recovery channel 5 is formed between the outer wall of the filter membrane column 3 and the inner wall of the accommodating cavity 13, and a sample outlet 7 is arranged at the bottom end of the recovery channel 5, wherein the filter membrane column 3 can convert cell filtration into a three-dimensional filtration process, so that the cell filtration efficiency is improved.

Wherein, the interface part 12 can connect the gun head body 1 with the pipette to make the pipette generate stable pressure and suck the cell suspension to be filtered into the sample introduction pipeline 10 through the sample introduction port 11 of the sample introduction pipeline 10 by the suction effect of the pipette, the cell suspension to be filtered in the sample introduction pipeline 10 flows out from the first valve 14 and is filtered by the filter membrane column 3, and the filtered cell suspension enters the recovery channel 5 and flows out from the sample outlet 7 so as to be convenient for the laboratory operator to collect the filtered cell suspension. The cooperation of rifle head body 1 and pipetting gun accords with laboratory operating personnel's operation custom, has simplified the cell filtration process, has avoided the use of measures such as extra atmospheric pressure, hydraulic pressure and centrifugation, has shortened the cell filtration time, has reduced the damage to the cell in the cell filtration process to cell filtration's efficiency has been improved to a certain extent.

During specific implementation, the filter membrane pore size in the filter membrane column 3 can be selected according to the actual filtration requirement of the cell suspension to be filtered, that is, when large impurities in the cell suspension need to be filtered, the filter membrane column 3 with large pore size can be selected; when the cell suspension needs to be screened, a filter membrane column 3 positioned between the pore diameters of two cells to be screened can be selected; when the cell suspension needs to be subjected to cell removal and virus separation, the filter membrane column 3 with small pore size is selected.

Referring to fig. 1, exemplarily, a pipette head body 1 is first loaded on a pipette gun through an interface part 12, and the pipette gun is ensured to be connected tightly so as to ensure the normal suction function of the pipette gun, and the pipette gun is pressed down to suck a buffer solution such as PBS compatible with a cell suspension to be filtered to wet the pipette head body 1 so as to discharge bubbles in the pipette head body 1; then pressing down the pipette gun, loosening and sucking the cell suspension to be filtered, transferring the gun head body 1 to a cell recovery container, pressing down the pipette gun, and discharging the impurity-removed cell sample obtained by filtering to the cell recovery container; and finally, pressing down the pipette, sucking buffer solutions such as PBS (phosphate buffer solution) and the like, and then discharging the buffer solutions to filter out residual cells remained in the pipette head body 1, so that the next cell filtration is facilitated.

According to the technical scheme, the cell filter provided by the embodiment is provided with the gun head body 1, the filter membrane column 3 and the sample introduction pipeline 10; the upper end of the gun head body 1 is provided with a connecting part 12 which is detachably connected with the pipette, an accommodating cavity 13 is formed between the lower end of the gun head body 1 and the lower end of the connecting part 12, a sample introduction pipeline 10 is arranged in the accommodating cavity 13, and the top end of the sample introduction pipeline 10 is provided with a first valve 14; the periphery of the sample inlet pipeline 10 is provided with a filter membrane column 3, two ends of the filter membrane column 3 are oppositely connected with the gun head body 1, a recovery channel 5 is formed between the outer wall of the filter membrane column 3 and the inner wall of the accommodating cavity 13, and a sample outlet 7 is arranged at the bottom end of the recovery channel 5. Set up like this, when treating filterable cell suspension and filtering, because rifle head body 1 is connected with the pipetting gun is detachable, be convenient for rifle head body 1 and the assembly connection of pipetting gun, also do benefit to the change of rifle head body 1 simultaneously, the suction through the pipetting gun makes the cell suspension of treating filteration inhale and holds in the chamber 13, and flow into between introduction pipeline 10 and filter membrane post 3 through introduction pipeline 10 and first valve 14, rethread filter membrane post 3 is treated filterable cell suspension, make the cell sample after the filtration get into recovery passageway 5 and flow out by outlet 7, the use of this cell filter cooperation pipetting gun, cell filtration's operation process has been simplified, high durability and convenient use, adopt filter membrane post 3 to filter the cell simultaneously, avoid among the cell filtration process impaired, and improved cell filtration's efficiency to a certain extent.

During the concrete implementation, the surface of introduction pipeline 10 is provided with liquid circulation structure 4, so set up, can carry out the water conservancy diversion to the cell suspension that treats the filteration that first valve 14 flows out to improve cell filtration's efficiency.

It can be understood that the sample introduction pipe 10 can be a circular pipe, and a circular hole matching with the circular pipe shape is formed inside the liquid circulation structure 4, so that the sample introduction pipe 10 is tightly matched with the liquid circulation structure 4; the sample introduction pipeline 10 can also be a square pipe, and a square hole matched with the square pipe in shape is formed in the liquid circulation structure 4; of course, the sample introduction pipeline 10 may also be a hole directly formed in the middle of the liquid circulation pipeline, and in the specific implementation, the hole may be arbitrarily selected according to the actual requirement, which is not limited herein.

Referring to fig. 1, the outer surface of the liquid flow structure 4 is in the shape of spiral teeth, the spiral teeth change the flow direction of the cell suspension to be filtered after flowing out from the first valve 14, and simultaneously increase the flow path of the cell suspension to be filtered on the outer surface of the liquid flow structure 4, so that the cell suspension forms tangential flow on the surface of the filter membrane column 3, the cross flow dynamics is applied to the cell filtration, the tangential flow can wash the inner wall of the filter membrane column 3 during the filtration, and impurities filtered out from the cell suspension are washed away to the inner wall away from the filter membrane column 3 in time, so that the pore size of the filter membrane column 3 is prevented from being blocked by the impurities, the normal filtration of the cell suspension by the filter membrane column 3 is ensured, and the cell filtration efficiency of the filter membrane column 3 is improved to a certain extent.

The number and shape of the teeth of the helical teeth can influence the tangential speed and the shearing force of the cell suspension to be filtered on the surface during flowing, and the specific size and structure of the helical teeth-shaped liquid flowing structure 4 can be adjusted according to actual requirements during specific use.

During concrete implementation, can be for tiny intermediate layer passageway between the outer wall of liquid flow structure 4 and the inner wall of filter membrane post 3, so set up, increased the contact range of waiting to filter cell suspension and filter membrane post 3 for the effective filter membrane area that cell suspension contacted in the filtration process increases, has increased the membrane machine meeting of waiting to filter cell suspension, thereby has improved the filtration efficiency of waiting to filter cell suspension.

In some embodiments, the end of the sample introduction tube 10 near the first valve 14 is lower than the top end of the liquid flow structure 4, and the top end of the first valve 14 is located above the top end of the liquid flow structure 4, so that the first valve 14 can be well fixed, so that the first valve 14 can effectively control the flow of the cell suspension to be filtered at the top end of the sample introduction tube 10.

In specific implementation, the second valve 8 is arranged at the sample outlet 7, and the first valve 14 and the second valve 8 are one-way valves, wherein the first valve 14 is set as the one-way valve, which can ensure one-way circulation of the cell suspension to be filtered, i.e. only allowing the cell suspension to be filtered to enter from the sample inlet 11 at the lower end of the sample introduction pipeline 10 and flow out from the top end of the sample introduction pipeline 10, and preventing the liquid outside the sample introduction pipeline 10 in the accommodating cavity 13 from reversely flowing into the sample introduction pipeline 10 from the top end of the sample introduction pipeline 10, thereby ensuring normal sample introduction of the sample introduction pipeline 10; similarly, the second valve 8 is set as a one-way valve, so that one-way circulation of the filtered cell sample in the recovery channel 5 can be ensured, that is, only the cell sample filtered by the filter membrane column 3 exists in the recovery channel 5, and it is avoided that unfiltered cell suspension or external air enters the recovery channel 5 when the gun head body 1 sucks the cell suspension to be filtered, so that normal filtration of the cell suspension to be filtered is ensured, thereby improving the purity of the filtered cell sample in the recovery channel 5, and further improving the filtering effect of the cell suspension to be filtered.

It should be understood that the opening and closing manner of the first valve 14 and the second valve 8 may be driven by the pressure during the normal circulation of the liquid, and the opening and closing manner of the first valve 14 and the second valve 8 may also be controlled in an electrically controlled manner, as long as the one-way opening and closing of the first valve 14 and the second valve 8 can be satisfied, which is not limited herein.

The gun head body 1 comprises an upper gun head 2 and a lower gun head 6, the upper gun head 2 is connected with the lower gun head 6 oppositely, and the upper gun head 2 is sequentially provided with a connector part 12 and an accommodating cavity 13 from top to bottom; the lower gun head 6 comprises a sample introduction pipeline 10 and a liquid circulation structure 4, and is convenient for assembling the gun head body 1 so as to filter the cell suspension to be filtered by the gun head body 1.

In this embodiment, the lower lance head 6 further includes a base 9, the base 9 is disposed at the bottom end of the liquid flow structure 4, and the base 9 and the liquid flow structure 4 are integrally formed, wherein the base 9 and the liquid flow structure 4 are integrally formed, so that the overall shape is good and the connection strength is high.

The outer wall that base 9 is close to the one end of liquid circulation structure 4 is connected relatively with the inner wall that holds chamber 13, base 9 specifically is sticky with the connected mode that holds the inner wall of chamber 13, be convenient for with last rifle head 2 with rifle head 6 assembly down together, the one end of filter membrane post 3 is connected relatively with one side that base 9 is close to liquid circulation structure 4, in order to ensure can carry out the integral filtration to the cell suspension of waiting to filter between the outer wall of liquid circulation structure 4 and the filter membrane post 3, the filtration efficiency of waiting to filter the cell suspension has been improved, introduction pipeline 10 runs through the setting of base 9 simultaneously.

Referring to fig. 1, the aperture that holds chamber 13 is greater than the aperture of interface part 12, the other end of filter membrane post 3 with hold chamber 13 and be close to the upper surface relatively fixed of interface part 12, set up like this, be convenient for fix the other end of filter membrane post 3 in holding chamber 13, cooperate being connected of 3 one end of filter membrane post and base 9, in order to carry out good fixed to filter membrane post 3, thereby improve filter membrane post 3 and treat the filter effect of filtering cell suspension, be convenient for simultaneously fixed filter membrane post 3 and hold and form confined recovery passageway 5 between the inner wall of chamber 13.

Wherein, the setting of outlet 7 is at base 9 and the coupling part who holds the intracavity 13 inner wall, and outlet 7 is one at least, and second valve 8 and outlet 7 one-to-one, during the concrete implementation, set up at least one outlet 7 and be favorable to discharging the cell suspension after filtering in the recovery passageway 5 and collect, of course, also can set up a plurality of outlets 7 to improve the recovery rate of the cell suspension after filtering, specifically can be according to actual demand and the quantity of the coupling part's of recovery passageway 5 department size adjustment outlet 7.

The outer diameters of the upper gun head 2 and the base 9 from top to bottom are gradually reduced at the position below the connecting part, so that the gun head body 1 can conveniently extend into a container for containing cell suspension to be filtered to absorb the cell suspension to be filtered, and it can be understood that when the container for containing the cell suspension to be filtered is deeper and/or has a smaller caliber, the length of the sample introduction pipeline 10 can also be correspondingly increased, so that the bottom end of the sample introduction pipeline 10 is lower than the lower end of the lower gun head 6, or the lower end of the sample introduction pipeline 10 and/or the lower end of the lower gun head 6 are connected with an additional pipeline which can deeply extend into the container for containing the cell suspension to be filtered, so that the cell suspension to be filtered can be absorbed conveniently, and the cell suspension to be filtered can be filtered.

It is noted that, in this document, relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The foregoing are merely exemplary embodiments of the present disclosure, which enable those skilled in the art to understand or practice the present disclosure. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the disclosure. Thus, the present disclosure is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (10)

1. A cell filter is characterized by comprising a gun head body (1), a filter membrane column (3) and a sample introduction pipeline (10);

the upper end of the gun head body (1) is provided with a connecting part (12) detachably connected with a pipette, an accommodating cavity (13) is formed between the lower end of the gun head body (1) and the lower end of the connecting part (12), the sample introduction pipeline (10) is arranged in the accommodating cavity (13), and the top end of the sample introduction pipeline (10) is provided with a first valve (14);

the periphery of introduction pipeline (10) is provided with filter membrane post (3), the both ends of filter membrane post (3) with rifle head body (1) is connected relatively, the outer wall of filter membrane post (3) with form between the inner wall that holds chamber (13) recovery passageway (5), the bottom department of recovery passageway (5) is provided with out appearance mouth (7).

2. The cell filter according to claim 1, characterized in that the outer surface of the sample inlet channel (10) is provided with a liquid flow-through structure (4).

3. The cell filter according to claim 2, characterized in that the external surface of the liquid flow-through structure (4) is shaped as a helical tooth.

4. The cell filter according to claim 2, wherein the end of the sample inlet tube (10) near the first valve (14) is lower than the top end of the liquid flow-through structure (4), and the top end of the first valve (14) is located above the top end of the liquid flow-through structure (4).

5. The cell filter according to claim 2, wherein a second valve (8) is provided at the sample outlet (7);

the first valve (14) and the second valve (8) are one-way valves.

6. The cell filter according to claim 5, characterized in that the lance tip body (1) comprises an upper lance tip (2) and a lower lance tip (6), the upper lance tip (2) being connected opposite to the lower lance tip (6);

the upper gun head (2) is sequentially provided with the interface part (12) and the accommodating cavity (13) from top to bottom; the lower gun head (6) comprises the sample inlet pipeline (10) and the liquid circulation structure (4).

7. The cell filter according to claim 6, characterized in that the lower lance head (6) further comprises a base (9), the base (9) is arranged at the bottom end of the liquid flow-through structure (4), and the base (9) is integrally formed with the liquid flow-through structure (4);

the outer wall of one end, close to the liquid circulation structure (4), of the base (9) is connected with the inner wall of the accommodating cavity (13) oppositely, and one end of the filter membrane column (3) is connected with one side, close to the liquid circulation structure (4), of the base (9) oppositely;

the sample introduction pipeline (10) penetrates through the base (9).

8. The cell filter according to claim 7, wherein the aperture of the accommodating chamber (13) is larger than that of the interface part (12), and the other end of the filter membrane column (3) is fixed opposite to the upper surface of the accommodating chamber (13) close to the interface part (12).

9. The cell filter according to claim 7, wherein the sample outlet (7) is provided at a connecting portion of the base (9) and an inner wall of the housing chamber (13);

the number of the sample outlets (7) is at least one, and the second valves (8) correspond to the sample outlets (7) one to one.

10. A cell filter according to claim 9, wherein the external diameters of the upper lance tip (2) and the base (9) taper from top to bottom at a location below the connection portion.

Images