CN216614646U

CN216614646U - Nucleic acid extraction kit by magnetic bead method

Info

Publication number: CN216614646U
Application number: CN202122666468.2U
Authority: CN
Inventors: 刘文佳; 马龙斌; 何东华; 刘蕊; 宋倩; 叶盛; 王欣浩; 周睿; 王思格
Original assignee: Hangzhou Lifereal Biotechnology Co ltd; Hangzhou Suizeng Biotechnology Co ltd; Shanghai Fuyuan Biotechnology Co ltd; Jiangsu Fuyuan Biotechnology Co ltd
Current assignee: Hangzhou Lifereal Biotechnology Co ltd; Hangzhou Suizeng Biotechnology Co ltd; Jiangsu Fuyuan Biotechnology Co ltd; Jiangsu Huayuan Biotechnology Co ltd
Priority date: 2021-10-29
Filing date: 2021-10-29
Publication date: 2022-05-27
Anticipated expiration: 2031-10-29

Abstract

The utility model discloses a nucleic acid extraction kit by a paramagnetic particle method, which comprises a kit body (1), wherein a sample adding cavity (2) and a mixing cavity (3) are arranged on the kit body (1), and a connecting liquid path (4) is arranged between the sample adding cavity (2) and the mixing cavity (3); the mixing cavity (3) is provided with an air hole (31), and the sample adding cavity (2) is provided with a sample adding hole (21). When the kit is used for extracting nucleic acid of a sample, only a plurality of steps need to be completed in one kit, external pollution is not easy to introduce, and the extraction efficiency can be greatly improved.

Description

Nucleic acid extraction kit by magnetic bead method

Technical Field

The utility model relates to a nucleic acid extraction kit by a paramagnetic particle method, and belongs to the technical field of medical detection.

Background

With the popularization of technical means such as gene detection and the like, the limitation of the traditional nucleic acid extraction method is very obvious today when high throughput and automation are pursued. At present, the magnetic bead method for extracting nucleic acid can realize automatic extraction and large-batch operation, is simple to operate and short in time, so that the magnetic bead method for extracting nucleic acid is more and more frequently used in the field of medical detection.

The general steps for extracting nucleic acids by the magnetic bead method are lysis or transformation, washing and elution. At present, a plurality of steps for extracting nucleic acid by a magnetic bead method are generally carried out in different test tubes or kits, a plurality of test tubes are required to be prepared, samples are required to be continuously transferred in the extraction process, external pollution is easily introduced, the operation is more complicated on the other hand, and the extraction efficiency is lower.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a nucleic acid extraction kit by a magnetic bead method. When the kit is used for extracting nucleic acid of a sample, only a plurality of steps need to be completed in one kit, external pollution is not easy to introduce, and the extraction efficiency can be greatly improved.

The technical scheme of the utility model is as follows: the kit for extracting nucleic acid by a paramagnetic particle method is characterized in that: the device comprises a box body, wherein a sample adding cavity and a mixing cavity are arranged on the box body, and a connecting liquid path is arranged between the sample adding cavity and the mixing cavity; the mixing chamber is provided with an air hole, and the sample adding chamber is provided with a sample adding hole.

In the kit for extracting nucleic acid by using the paramagnetic particle method, the connecting liquid path is respectively connected with the lower ends of the sample adding cavity and the mixing cavity.

In the kit for extracting nucleic acid by the paramagnetic particle method, the lower ends of the sample adding cavity and the mixing cavity are provided with inverted triangular pyramids. Wherein the inverted triangular cone area of the lower end of the mixing cavity is used as a magnetic bead adsorption area for adsorbing magnetic beads in the funnel-shaped area when the magnetic beads are close to the magnet.

In the kit for extracting nucleic acid by using the magnetic bead method, the bottoms of the sample adding cavity and the mixing cavity are obliquely guided and connected with the liquid path.

Compared with the prior art, the kit provided by the utility model has the advantages that the two chambers communicated by the connecting liquid path are respectively used as the sample adding chamber and the mixing chamber, the solution added into the sample adding chamber can freely move between the two chambers by utilizing the pneumatic principle, the liquid transfer can be realized, and the liquid can be uniformly mixed by repeatedly transferring the liquid back and forth. Therefore, the kit can sequentially carry out the steps of magnetic bead combination, one-time or multiple-time cleaning, elution and the like on the sample, different reagents are sequentially added into the sample adding cavity, sample nucleic acid does not need to be transferred, the whole extraction process can be finished in one reagent kit, the pollution of an external machine is not easy to introduce, the operation is simple, and only the action of an air pump and the addition/derivation of the reagents need to be controlled.

Drawings

FIG. 1 is a schematic structural diagram of a kit according to example 1 of the present invention;

FIG. 2 is a schematic structural diagram of a kit according to example 2 of the present invention.

Detailed Description

The utility model is further illustrated by the following figures and examples, which are not to be construed as limiting the utility model.

Example 1: a nucleic acid extraction kit by a magnetic bead method is shown in figure 1: the device comprises a box body 1, wherein a sample adding cavity 2 and a mixing cavity 3 are arranged on the box body 1, and a connecting liquid path 4 is arranged between the sample adding cavity 2 and the mixing cavity 3; the mixing cavity 3 is provided with an air hole 31, and the sample adding cavity 2 is provided with a sample adding hole 21. The connecting liquid path 4 is respectively connected with the lower ends of the sample adding cavity 2 and the mixing cavity 3. The lower ends of the sample adding cavity 2 and the mixing cavity 3 are provided with inverted triangular pyramids. The bottom parts of the sample adding cavity 2 and the mixing cavity 3 are inclined, guided and connected with a liquid path 4.

The extraction method of the nucleic acid extraction kit by using the magnetic bead method comprises the following steps:

adding a binding solution, a magnetic bead solution and a sample solution subjected to cracking or conversion into a sample adding cavity 2 from a sample adding hole 21;

the air hole 31 is connected with an air pump, the solution added from the sample adding cavity 2 flows back and forth between the mixing cavity 3 and the sample adding cavity 2 by controlling the positive pressure and the negative pressure of the air pump in a reciprocating way, and all the solution is sucked into the mixing cavity 3 after the solution is fully and uniformly mixed;

and thirdly, the magnet absorbs the magnetic beads in the solution in the magnetic bead adsorption area 32 of the mixing cavity 3 through magnetic force, the magnet stands for a period of time, and after the solution in the mixing cavity 3 becomes clear, the air pump pumps air to extrude the supernatant to the sample adding cavity 2 and move the supernatant away by using a pipettor.

Fourthly, adding quantitative cleaning fluid into the sample adding cavity, electromagnetically releasing magnetic beads, and repeating the second step and the third step.

And fourthly, repeating the step IV by using different or same cleaning liquids for multiple times according to requirements.

Fifthly, eluent is added into the sample adding cavity 2, the magnetic beads are released by the magnet, and the second step is repeated;

sixthly, the magnet attracts the magnetic beads, standing for a period of time, after the solution in the mixing cavity 3 becomes clear, pumping air by an air pump, extruding the supernatant into the sample adding cavity 2, and extracting the solution into a specified test tube by a pipettor.

Example 2: the kit for extracting nucleic acid by the magnetic bead method is shown in figure 2: the difference from example 1 is that there is an addition well 33 above the mixing chamber for adding the magnetic bead solution, and the well must be sealed after addition.

The specific operation steps are as follows:

adding a binding solution and a sample solution subjected to cracking or conversion into a sample adding cavity 2 from a sample adding hole 21; adding a magnetic bead solution into the mixing chamber from the well 33, and sealing the well 33;

the air hole 31 is connected with an air pump, and the solution in the sample adding cavity 2 is pumped into the mixing cavity 3 to be mixed with the magnetic bead solution by controlling the positive pressure and the negative pressure of the air pump;

thirdly, the magnet sucks the magnetic beads in the solution into the magnetic bead adsorption area 32 of the mixing cavity 3 through magnetic force, the mixture is kept still for a period of time, after the solution in the mixing cavity 3 becomes clear, the air pump pumps air, and the supernatant is extruded to the sample adding cavity 2;

fourthly, repeating the steps II and III for a plurality of times, extruding the supernatant into the sample adding cavity 2 and then removing the supernatant by a liquid transfer device.

Fifthly, adding quantitative cleaning liquid into the sample adding cavity, electromagnetically releasing magnetic beads, and repeating the steps II, III and IV.

Repeating the above steps with different or same cleaning solution for several times according to requirement.

Sixthly, eluent is added into the sample adding cavity 2, the magnetic beads are released by the magnet, and the step II and the step III are repeated;

and seventhly, attracting the magnetic beads by a magnet, standing for a period of time, pumping by an air pump after the solution in the mixing cavity 3 becomes clear, extruding the supernatant into the sample adding cavity 2, and extracting the solution into a specified test tube by using a pipettor.

The advantages of this method are: the magnetic beads are always in the mixing cavity, so that the magnetic beads are prevented from remaining in other chambers or liquid paths, and the nucleic acid extraction efficiency is prevented from being reduced.

Claims

1. A nucleic acid extraction kit by a paramagnetic particle method is characterized in that: comprises a box body (1), wherein a sample adding cavity (2) and a mixing cavity (3) are arranged on the box body (1), and a connecting liquid path (4) is arranged between the sample adding cavity (2) and the mixing cavity (3); the mixing cavity (3) is provided with an air hole (31), and the sample adding cavity (2) is provided with a sample adding hole (21).

2. The kit for nucleic acid extraction by the magnetic bead method according to claim 1, wherein: and the connecting liquid path (4) is respectively connected with the lower ends of the sample adding cavity (2) and the mixing cavity (3).

3. The kit for nucleic acid extraction by the magnetic bead method according to claim 1, wherein: and the lower ends of the sample adding cavity (2) and the mixing cavity (3) are provided with inverted triangular conical surfaces.

4. The kit for nucleic acid extraction by the magnetic bead method according to claim 1, wherein: the bottom parts of the sample adding cavity (2) and the mixing cavity (3) are inclined, guided and connected with the liquid path (4).