CN216771264U

CN216771264U - Sample dilution tube and sampling kit using same

Info

Publication number: CN216771264U
Application number: CN202220267140.5U
Authority: CN
Inventors: 潘志远; 厉刚; 毕玉晶; 杨瑞馥
Original assignee: Beijing Junli Kangbo Technology Co ltd; Academy of Military Medical Sciences AMMS of PLA
Current assignee: Beijing Junlikang Technology Development Co ltd; Academy of Military Medical Sciences AMMS of PLA
Priority date: 2022-02-09
Filing date: 2022-02-09
Publication date: 2022-06-17
Anticipated expiration: 2032-02-09

Abstract

The utility model provides a sample dilution tube and a sampling kit using the same. The sample dilution tube includes: a pipe body; the sealing piston can be freely and slidably sleeved on the inner side of the pipe body; the sealing cover is arranged on the upper part of the pipe body in a covering manner; wherein, enclose into the diluent space between body of pipe, sealing piston, the closing cap, when the sample squeezes into the diluent in the diluent space, sealing piston can independently slide and realize the grow of diluent space volume. The utility model avoids the opportunity that the sample and the diluent are contacted with the outside air to the maximum extent, maintains the anaerobic environment and improves the separation culture efficiency of anaerobic and facultative anaerobic microorganisms in the excrement sample.

Description

Sample dilution tube and sampling kit using same

Technical Field

The utility model relates to the field of medical instruments, in particular to a sample diluting tube and a sampling kit applying the same.

Background

The intestinal tract contains a large number of microorganisms which are closely related to the health degree of animals, and the research on the intestinal tract microorganisms aims to understand the symbiotic relationship between animals and microorganisms and the process relationship between microbial flora change and diseases, thereby laying a theoretical foundation for developing and utilizing the microorganisms to improve the health of the human body and the animals. The research of intestinal microorganisms is a common technical means in the research of intestinal microorganisms, which is to generally obtain effective information through the research of fecal microorganisms, sample and dilute the feces, and further obtain microbial species through isolated culture. The quantitative sampling and the quantitative dilution of the fecal specimen are beneficial to effectively judging the species and the abundance of various microorganisms in the intestinal tract.

In the process of detecting the fecal sample, the sample is firstly quantitatively collected and uniformly mixed with the sample diluent, so that the sample adding detection work can be carried out. However, anaerobic and facultative anaerobic microorganisms in the intestinal tract are easily killed by the oxygen in the air after the feces are discharged out of the body, so that it is important to maintain an effective anaerobic environment during sampling. However, the current fecal sampler cannot usually maintain an effective anaerobic environment, and the culture and separation effect of anaerobic and facultative anaerobic microorganisms in fecal microorganism research is seriously affected.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The present invention is intended to solve at least one of the above technical problems at least in part.

(II) technical scheme

To achieve the above object, according to one aspect of the present invention, there is provided a sample dilution tube including: a tube body; the sealing piston can be freely and slidably sleeved on the inner side of the pipe body; the sealing cover is arranged on the upper part of the pipe body in a covering manner; wherein, enclose into the diluent space between body of pipe, sealing piston, the closing cap, when the sample squeezes into the diluent in the diluent space, sealing piston can independently slide and realize the grow of diluent space volume.

In some embodiments of the utility model, further comprising: the sampling needle is fixed at the bottom of the sealing piston, a sampling channel is formed in the sampling needle, the sampling channel is upwards communicated with the diluent space, and the needle head of the sampling needle penetrates through the sealing piston and extends to the inner space of the tube body at the lower part of the sealing piston; the elastic sealing sleeve is sleeved outside the needle head of the sampling needle.

In some embodiments of the utility model, further comprising: and the second filter sheet is arranged on the upper part of the sealing piston and at least covers the opening of the sampling channel facing the diluent space.

In some embodiments of the utility model, the pore size of the second filter is between 80 and 400 mesh.

In some embodiments of the utility model, further comprising: and the bottom cover is detachably covered at the bottom of the tube body, and the sealing piston with the sampling needle is packaged at the inner side of the tube body.

In some embodiments of the utility model, the closure cap is recessed downwardly to form a dosing chamber, the dosing chamber being in communication with the diluent space; the sample dilution tube further comprises: the middle part of the top cover is downwards protruded to form an extrusion column, and the front end of the extrusion column is matched with the position and the shape of the quantitative cavity; when the top cover is gradually pressed down, the extrusion column and the quantitative cavity form plunger matching, and a sample in the quantitative cavity is extruded into the diluent.

In some embodiments of the utility model, the cover is recessed downwardly outside the dosing chamber to form an overflow chamber.

In some embodiments of the utility model, the dosing chamber is cylindrical; the overflow chamber is annular and surrounds the periphery of the quantitative cavity.

In some embodiments of the present invention, the outer circumferential side of the pipe body or the cap is formed with an external thread; the inner periphery side of the cover body of the top cover is provided with an internal thread which is matched with the external thread; when the quantitative cavity is not used, the bottom of the external thread is provided with a limiting ring, so that the internal thread cannot be screwed to the bottom of the external thread, and the front end of the extrusion column is positioned at the outer side of the quantitative cavity; when the quantitative liquid sample taking device is used, the limiting ring is removed, the limiting ring rotates downwards along with the engagement of the internal thread and the external thread, the extrusion column on the top cover is gradually extruded into the quantitative cavity, and a sample with a corresponding volume is extruded into a diluent space.

In some embodiments of the utility model, the body is integrally formed with the cover.

In some embodiments of the present invention, the tube body is made of a transparent and translucent material.

In some embodiments of the utility model, further comprising: one or more grinding balls are accommodated in the diluent space.

In some embodiments of the utility model, the diluent is deoxygenated prior to filling and is filled under anaerobic conditions.

To achieve the above object, according to a second aspect of the present invention, there is also provided a sampling kit comprising: a sample dilution tube as above; the sampling tube is a vacuum tube, the top of the sampling tube is covered with an elastic plug, and the outer diameters of the sampling tube and the elastic plug are smaller than the inner diameter of the tube body of the sample diluting tube; wherein, in the sample transfer process, the sampling needle punctures the elastic plug; the elastic plug pushes the sealing piston upwards to slide upwards, so that the diluent containing the sample in the diluent space is squeezed into the sampling tube.

(III) advantageous effects

According to the technical scheme, the utility model has at least one of the following beneficial effects:

(1) a diluent space is enclosed among the tube body, the sealing piston and the sealing cover, the diluent is kept in a closed state in the diluent space at first, then after the sample is sent into the diluent, the sealing piston can independently slide downwards to enlarge the diluent space, the opportunity that the sample and the diluent are contacted with the outside air is avoided to the maximum extent, the anaerobic environment is kept, and the separation culture efficiency of anaerobic and facultative anaerobic microorganisms in the excrement sample is improved.

(2) The sampling needle is arranged on the sealing piston, the elastic sealing sleeve tightly connected with the sealing piston is arranged on the outer side of the sampling needle to form a sealing structure, when the excrement sample is squeezed into the diluent space, the sealing piston moves downwards, and the diluent cannot flow out of the sampling channel in the sampling needle. During sampling, under the upward thrust action of the sampling tube, the needle head of the sampling needle punctures the elastic sealing sleeve and the rubber plug at the top of the sampling tube, and the sample diluent enters the sampling tube under the action of the thrust and/or the vacuum suction. When sampling is finished, the elastic sealing sleeve recovers the wrapping state of the needle under the action of the elastic force, and the phenomenon that the sample flows out or air enters to influence the next sample collection is avoided.

(3) The middle part of the sealing cover is sunken downwards to form a quantitative cavity, under the action of the extrusion column, a sample with the volume limited by the quantitative cavity can be extruded into a diluent space, and a quantitative excrement sample is obtained through volume control, so that the controllability of the concentration of the excrement sample diluent is ensured, and the quantitative excrement sample diluent is more convenient and accurate.

(4) The outer side of the pipe body is provided with an external thread, the inner side of the top cover is provided with an internal thread matched with the external thread, and when the pipe body is not used, the bottom of the outer side of the external thread is provided with a limiting ring, so that the internal thread can not be screwed to the bottom of the external thread, and the front end of the extrusion column is positioned on the outer side of the quantitative cavity; in a use state, the limiting ring is removed, the internal thread is screwed downwards to the bottom of the external thread, and the extrusion column on the top cover extrudes the sample in the quantitative cavity downwards to extrude the sample into the diluent space.

(5) Further, the volume V of the stool sample entering the diluent is the cross-sectional area S of the quantification chamber and the height H of the stop collar. The change of the sampling volume can be realized by replacing the limiting rings with different heights or the combination of the limiting rings.

(6) And the sealing cover is sunken downwards to form an overflow chamber outside the quantitative cavity, so that excessive fecal samples can be squeezed into the overflow chamber from the quantitative cavity, and the pollution caused by the fecal samples overflowing from other positions is avoided.

(7) In an unused state, the quantitative cavity is covered with a sealing film to ensure that oxygen in the air can not enter the inside of the sample dilution tube before sampling. When the quantitative anaerobic bacteria dilution device is used, the sealing film is torn, the sample is filled into the quantitative cavity and enters the dilution liquid through extrusion, and therefore oxygen in the air is prevented from entering the dilution system to affect the activity of anaerobic bacteria.

(8) Sampling kit's whole still includes the sampling pipe, uses with the cooperation of above-mentioned sampling needle, and the sampling pipe utilizes sealed piston can be in the inboard gliding characteristics of pipe shaft when in actual use, and the elasticity stopper on sampling pipe upper portion promotes sealed piston and upwards slides to squeeze into the sampling pipe with the diluent of the excrement and urine sample in the diluent space in, and the ration sampling of diluent is realized to the distance of moving up of accessible sealed piston and sampling pipe.

(9) The tube body of the sample dilution tube and the sampler can be made of transparent and semitransparent materials, so that a tester can conveniently observe the state of the internal sample dilution liquid.

(10) Set up first filter plate at the top in diluent space, set up the second filter plate in the bottom, first filter plate effect is for tentatively filtering the animal and plant fibre and the large granule food waste that do not have the research meaning in the excrement, and second filter plate aperture is less than the aperture of sampling needle sampling channel, avoids the suspended solid of particles to block sampling channel.

(11) The grinding balls in the diluent space can grind the excrement sample entering the diluent space, so that flora in the excrement sample is uniformly dispersed in the diluent, and the uniformity of the diluted sample is improved.

(12) And (3) carrying out deoxidization operation before filling the diluent, and filling the diluent in an anaerobic environment to ensure that no dissolved oxygen exists in the diluent. Meanwhile, after the sample is filled into the quantitative cavity, the top cover is immediately covered, and after the sample is extruded into the diluent space, the extrusion column and the quantitative cavity form plunger matching to completely seal the upper part of the tube body, and the elastic sealing sleeve at the lower part of the tube body sleeves the lower part of the sampling needle, so that the whole diluent space is kept in an anaerobic environment, and external oxygen is prevented from entering an anaerobic state which damages the liquid diluent as far as possible.

Drawings

Fig. 1A, 1B and 1C are a perspective view, a plan view and a front view of a stool sample dilution tube according to an embodiment of the present invention before use, respectively.

Fig. 2 is a cross-sectional view of the stool sample dilution tube shown in fig. 1C taken along the direction a-a.

Fig. 3 is a cross-sectional view of the fecal sample dilution tube of fig. 1C after use.

Fig. 4A, 4B and 4C are a perspective view, a plan view and a front view of a fecal sampling kit according to an embodiment of the present invention, respectively.

Fig. 5 is a cross-sectional view in the direction B-B of a portion of the diluent in the fecal sampling kit of fig. 4C after transfer to the sampling tube.

[ description of main element symbols in the drawings ]

100. Excrement sample dilution tube

110. A tube body;

120. sealing the cover; 121. a dosing chamber; 122. an overflow chamber; 123. a sample introduction channel;

130. a top cover; 131. extruding the column; 132. a limiting ring;

140. a sealing piston;

150. a diluent space; 151. a first filter sheet; 152. a second filter sheet; 153. grinding balls;

160. a sampling needle; 161. a rubber protective sleeve;

170. a bottom cover;

210. a sampling tube; 220. an elastic plug;

l is the length of the sampling tube;

h0 is the height of the diluent space when no sample is added to the diluent;

h1 is the height of the diluent space after the sample is added to the diluent;

h2 is the height of the diluent space after transfer of a portion of the diluent to the sample tube;

t is the thickness of the sealing piston of the sample dilution tube.

Detailed Description

The utility model provides an intestinal microorganism sampling device for finishing the treatment process of a fecal sample under a relatively anaerobic condition, and can meet the research requirement of intestinal anaerobic microorganisms. Before the description of the specific embodiments, it should be noted that:

(1) although most of the embodiments are described by taking a stool sample as an example, it should be understood by those skilled in the art that the sample dilution tube and the corresponding sampler of the present invention can be applied to the detection of anaerobic microorganisms in other application scenarios, such as: detection of certain cultural relics in archaeological applications.

(2) It will be appreciated by those skilled in the art that the sample dilution tube and corresponding sampling kit of the present invention are equally applicable to the detection of other mammalian fecal samples.

(3) The main innovation points of the utility model include but are not limited to the following two points: firstly, the diluent space can be self-adaptive to the addition of the sample; ② the dosing chamber may control the amount of sample entering the diluent space. The two innovation points are independent from each other and can be independently adopted. It will be appreciated by those skilled in the art that the sample dilution tube can employ both of the above innovations, and can also employ only one of the innovations, to achieve the corresponding advantages.

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to specific embodiments and the accompanying drawings.

In a first exemplary embodiment of the present invention, a fecal sample dilution tube is provided.

Fig. 1A, 1B and 1C are a perspective view, a plan view and a front view of a stool sample dilution tube according to an embodiment of the present invention before use, respectively. Fig. 2 is a cross-sectional view of the stool sample dilution tube shown in fig. 1C taken along the direction a-a. Fig. 4A, 4B and 4C are a perspective view, a plan view and a front view of a fecal sampling kit according to an embodiment of the present invention, respectively. Fig. 5 is a cross-sectional view in the direction B-B of a portion of the diluent in the fecal sampling kit of fig. 4C after transfer to the sampling tube.

Referring to fig. 4A, 4B, 4C and 5, the stool sampling kit includes: a fecal sample dilution tube 100 and a sampling tube 210. In the dilution of the stool sample, only the stool sample dilution tube 100 is used. Work is saved in the sample that uses the realization sample after diluting through both cooperations, need get rid of the sample at this moment and dilute the tube bottom, and sampling pipe 210 gos deep into in the middle of the pipe shaft of excrement and urine sample dilution pipe, and rubber protective sheath and sampling pipe plug are punctured to the sampling needle, upwards promote sealed piston, and excrement and urine sample diluent gets into through the sampling needle and shifts in the pipe.

With continuing reference to fig. 1A, 1B, 1C and 2, a stool sample dilution tube 100 according to an embodiment of the present invention includes:

a tube body 110 in a sleeve shape;

a sealing cover 120 which is covered above the tube body and is provided with a quantitative cavity 121, an overflow chamber 122 and a sample feeding channel 123 at the upper part;

a top cover 130 covering the top of the cover 120 and extending downward to form an extrusion column 131, the front end of which matches with the position and shape of the quantitative cavity;

the sealing piston 140 is freely and slidably sleeved on the inner side of the tube body, and a diluent space 150 is defined among the tube body 110, the sealing piston 140 and the sealing cover 120;

the sampling needle 160 is fixed on the sealing piston, a sampling channel is formed in the sampling needle, the sampling channel is upwards communicated with the diluent space, the needle head of the sampling needle penetrates through the sealing piston and extends to the inner space of the tube body at the lower part of the sealing piston, and the outer side of the needle head is sleeved with an elastic sealing sleeve;

and a bottom cover 170 detachably covering the bottom of the tube body and enclosing the sealing piston 140 and the sampling needle 160 in the inner space of the tube body.

The following describes each component of the fecal sample diluting tube 100 of this embodiment in detail.

First, the body 110 is a tubular structure having a smooth inner wall, and a piston space for the movement of the sealing piston 140 is formed inside. The upper part of the tube body is provided with a sealing cover 120. The tube body 110, the sealing piston 140 and the cover 120 enclose a diluent space 150. The diluent space 150 is filled with a diluent in advance. The sealing piston 140 is tightly fitted with the inner side wall of the tube body, and the adjustment of the spatial volume of the diluent is realized through up-and-down sliding.

In this embodiment, the diluent in the diluent space is an aseptic deoxygenation diluent for diluting the fecal sample to be tested. The sterile deoxygenated diluent is filled in an anaerobic environment and deoxygenated prior to filling. And the gas in the diluent space is exhausted before the sample dilution tube is sealed. For the information about the diluent, reference may be made to the related description of the prior art, and the description thereof is omitted here.

During sampling, the top cover 130 is taken down from the tube body 110, pasty fecal samples are filled into the quantitative cavity 121 on the sealing cover, then the top cover 120 is covered again and screwed gradually, in the process of downward movement of the top cover 130, the fecal samples in the quantitative cavity 121 are extruded by the extrusion column, enter the diluent space through the sample introduction channel 123, and by shaking, the fecal particles are scattered by the grinding balls and diluted in the diluent space.

After the excrement sample enters the diluent space through the sample introduction channel, the volume of the diluent is increased due to the addition of the excrement sample, and the sealing piston 140 automatically slides downwards on the inner side of the tube body to enlarge the diluent space. Comparing fig. 2 and fig. 3, the height of the diluent space before entering the stool sample was H0 and after being squeezed into the stool sample was H1, H1 > H0.

In this process, excrement and urine sample and air contactless to reduce excrement and urine sample and diluent and outside air contact's chance, protect anaerobic microorganisms activity, promoted anaerobism and facultative anaerobic microorganisms's survival probability in the excrement and urine sample.

In conclusion, in the embodiment, a diluent space is defined among the tube body, the sealing piston and the sealing cover, the diluent is kept in a closed state in the diluent space at first, and then after the sample is sent into the diluent, the sealing piston can automatically slide downwards to expand the diluent space, so that the chance that the sample and the diluent are in contact with the outside air is minimized, the anaerobic environment is kept, and the accuracy of detection of anaerobic and facultative anaerobic microorganisms in the fecal sample is improved.

Next, a dosing chamber 121 and an overflow chamber 122 are formed in the cap 120. Since the cap and the tube body are both made of resin material, it is very convenient to form the cavity by forming the recess thereon.

The cover is sunken downwards to form a quantitative cavity 121, the quantitative cavity 121 is cylindrical, the upper diameter and the lower diameter of the quantitative cavity are the same, the material is slightly elastic and can be tightly matched with the extrusion column, and the sealing of the upper part of the diluent space is facilitated. Under the effect of extrusion post 131, the excrement sample of the volume of being injectd by the holding ring height can be crowded into diluent space 150, and during unnecessary excrement sample then can not get into the sample diluent, obtains quantitative excrement sample through volume control, has guaranteed the controllability of excrement sample diluent concentration, and is more convenient and accurate.

The bottom of the dosing chamber is provided with a first filter 151. The aperture of the first filter sheet is between 30 and 400 meshes. In this embodiment, the aperture of the first filter sheet is 50 meshes. This first cassette 151 can treat the excrement and urine sample of separation and carry out prefilter, avoids not having animal and plant fiber and the large granule food waste of research significance to get into the diluent space, reduces and detects the degree of difficulty, improves the accuracy that detects.

Outside the dosing chamber, the cover is recessed downwards forming an annular overflow chamber 122. The overflow compartment 122 is intended to collect a stool sample outside the volume defined by the dosing chamber. Through the arrangement, the excessive excrement sample can be squeezed into the overflow chamber from the quantitative cavity, and the pollution caused by the excrement sample overflowing from other positions is avoided.

Before sampling, the quantitative cavity 121 is covered with a sealing film to ensure that oxygen in the air cannot enter the inside of the sample dilution tube before sampling. When the sample is to be sampled, the top cover is opened, the sealing film is uncovered, the stool sample is filled in the quantitative cavity, and then the top cover is covered. Therefore, the diluent and the excrement sample are in short contact with the outside only at the moment of filling the sample, so that the phenomenon that the outside oxygen is dissolved into the diluent to the maximum extent is avoided, and the phenomenon that the oxygen in the air enters a dilution system to influence the activity of anaerobic bacteria is avoided.

Thirdly, external threads are formed on the outer side of the sealing cover in the circumferential direction; the inner side of the cover body of the top cover is circumferentially provided with an internal thread which is matched with the external thread. In an unused state, the bottom of the outer side of the external thread is provided with a limiting ring 132, so that the internal thread cannot be screwed to the bottom of the external thread, the front end of the extrusion column 131 is positioned at the outer side of the quantitative cavity 121, and the extrusion column 131 is prevented from excessively extruding the sealing film on the quantitative cavity. Before sampling, the stop collar 132 is removed and the cap 130 is unscrewed. After sampling, the top cap is screwed on again, the internal thread is screwed down to the bottom of the external thread, so that the extrusion column on the top cap extrudes the sample in the quantitative cavity downwards into the diluent space, as shown in fig. 3. Through the combined arrangement, the quantitative sampling of the excrement sample dilution pipe can be realized.

It will be appreciated by those skilled in the art that the depth of the cover unscrewing can be used to control the volume of sample expressed into the diluent space, the volume of fecal sample entering the diluent, V, the cross-sectional area of the dosing chamber, S, and the height of the stop collar, H. The sampling volume can be changed by replacing the limiting rings with different heights or the combination of the limiting rings.

In addition, although the external thread is disposed on the top cover portion in this embodiment, in other embodiments of the present invention, the external thread may also be disposed on the tube body, and the effects of the present invention may also be achieved, which is not described herein again.

In the embodiment, the sampling needle 160 is fixed to the sealing piston, a sampling channel is formed in the sampling needle, the sampling channel is upwards communicated with the diluent space, and the needle head of the sampling needle penetrates through the sealing piston and extends to the inner space of the tube body at the lower part of the sealing piston; the elastic sealing sleeve 161 is sleeved on the outer side of the sampling needle extending out of the needle head of the sealing piston and tightly connected with the sealing piston to form a closed structure. The sampling needle 160 is used in conjunction with a subsequent sampling tube 210. The sampling needle 160 may be made of hard plastic or metal.

The sampling passage of the sampling needle 160 does not affect the tightness of the diluent space due to the elastic protection of the elastic sealing sleeve 161. Since the sealing piston 140 is freely slidable along the inner side of the tube body, when the stool sample is squeezed by the squeezing column 131, the diluent does not flow out of the sampling channel, but the sealing piston 140 is forced to slide downward to increase the volume of the diluent space.

As mentioned above, the needle of the sampling needle extends through the sealing piston to the space inside the tube body below the sealing piston. The bottom cover 170 is detachably covered at the bottom of the tube body, and the needle head of the sampling needle is packaged inside the tube body. During transportation and sampling, the bottom cover 170 is covered at the bottom of the tube body. After sampling is completed, the bottom cap 170 is removed and the sampling tube 210 is inserted. The bottom cap 170 may prevent accidental injury from the sampling needle.

With continued reference to fig. 1A, 1B, 1C and 2, a second filter 152 is further disposed at the upper portion of the sealing piston in the diluent space. The second filter 152 covers at least the opening of the sampling channel facing the diluent space, and the aperture thereof is between 80 and 400 meshes, in this embodiment 100 meshes. This second cassette can filter the large granule magazine in the excrement and urine sample diluent, only lets the diluent that has the microorganism of testing value get into sampling tube, avoids blockking the sampling passageway of sampling needle.

In addition, a plurality of grinding balls 153 are also contained in the diluent, and are accommodated in the diluent space. The grinding balls can be glass, ceramic, stainless steel or other materials which have density higher than that of the diluent and have no influence on the activity of the microorganisms, and the glass balls are adopted in the embodiment. These grinding balls can break up the excrement sample that gets into in the diluent space, promote microorganism evenly distributed wherein, and the abundant dilution realizes the mixing of excrement sample in the sampling pipe, promotes the homogeneity of sample after diluting.

Finally, the fecal sample dilution tube of this embodiment has the following two points to be explained:

(1) in this embodiment, the cap 120 is integrally formed with the body 110. However, in other embodiments of the present invention, the cover 120 and the tube body 110 may be formed separately and then connected together by gluing, screwing, etc. The forming and connecting manner of the two can be understood by those skilled in the art, and will not be described in detail herein.

(2) In this embodiment, in order to facilitate observation of the state of the diluent and the sample in the diluent, the tube body is designed so far, and the introduction of the fecal sample dilution tube in the embodiment of the present invention is completed.

It should be noted that although the present embodiment is described by taking the stool sample dilution tube as an example, the sample dilution tube of the present invention can be applied to other application scenarios, for example, detection of anaerobic and facultative anaerobic microorganisms in environments such as archaeology and deep sea.

In a second exemplary embodiment of the present invention, a fecal sampling kit is also provided.

Referring to fig. 4A, 4B, 4C and 5, the fecal sampling kit includes: the sample dilution tube 100 as described above; and a sampling tube 210, the top of which is covered with an elastic plug 220. Wherein the sampling tube 210 and the elastic plug 220 have an outer diameter smaller than an inner diameter of the body of the sample dilution tube. The elastic plug 220 is a soft material that can rebound after piercing, and is preferably made of rubber.

In the diluent transfer process, the bottom cap 170 of the sample dilution tube is removed, the sampling tube 210 is pushed into the inner space of the tube body, the sampling needle 160 pierces the elastic plug 220 to extend into the sampling tube, and the sample diluent is sucked or squeezed into the sampling tube 210 through the sampling passage. Comparing fig. 3 and 5, the height of the diluent space after being squeezed into the stool sample is H1, and after a portion of the diluent is squeezed into the sampling tube, the height of the diluent space changes to H2.

In order to mix the diluent in the diluent space into the fecal sample, the diluent can completely enter the sampling tube 200, and the sample dilution tube 100 and the sampling tube 210 satisfy the following conditions:

L≥H1-T

wherein L is the length of the sampling tube, H1 is the height of the diluent space after addition of the stool sample, and T is the thickness of the sealing piston of the stool sample dilution tube.

It should be noted that the sampling tube 210 can determine the sampling volume by adjusting the vacuum level during the production process. Reagent components capable of removing residual oxygen and nutrient components capable of maintaining microbial bioactivity can be added into the sampling tube, so that the anaerobic microorganisms in the sample can normally survive.

It can be seen that this embodiment has utilized the characteristics that sealed piston can be in the inside activity from top to bottom of pipe shaft again, and the elastic plug on sampling pipe upper portion promotes sealed piston and upwards slides to squeeze into the sampling pipe with the diluent of the excrement and urine sample in the diluent space, promoted the flexibility that the excrement and urine sample thief used greatly.

Referring to fig. 2, 3 and 5, the process of using the fecal sampling kit of this embodiment is fully described as follows:

(1) the sampling sheet is a piece of wood loading sheet and is used for picking up a fecal sample; the fecal sample dilution tube is shown in fig. 2;

(2) the top cover 130 and the limiting ring 132 are removed, the sealing film is uncovered, the sampling sheet is used for picking up the fecal sample and adding the fecal sample into the quantitative cavity 121, the top cover 130 is covered, the extrusion column 131 of which the top cover is sunken downwards is extruded into the quantitative cavity 121 by rotating the top cover 130, and due to the action of the first filter sheet 151, the part with better fluidity in the sample to be detected is extruded into the diluent space 150, as shown in fig. 3.

(3) Vibrating the bottle body, and grinding and uniformly mixing the excrement sample by using a glass ball;

(4) the lower cap is removed and the sampling tube 210 is pushed in from the bottom end and the elastomeric stopper 220 of the sample transfer tube pierces the elastomeric sealing boot at the sampling needle tip location. The needle of the sampling needle 160 pierces the elastomeric stopper of the sample transfer tube, the diluted sample diluent flows into the sampling tube under vacuum, and the sealing piston 140 of the sample diluent tube slides up the tube as the diluent volume 150 decreases.

(5) To more completely express the sample tube in the diluent, the sample tube may also be pushed upward, causing the sealing piston 140 to continue moving upward, completely expressing the sample diluent in the diluent space into the sample tube, as shown in fig. 5. After the sampling process is completed, the sampling tube is pulled out to complete the sampling process.

So far, the introduction of the feces sampling kit is finished in the embodiment of the utility model.

So far, the detailed description has been given of the embodiments of the present invention with reference to the accompanying drawings. The present invention should be clearly recognized by those skilled in the art from the above description.

In conclusion, the utility model provides the sample diluting tube and the sampling kit which have good anaerobic protection effect on the sample, so that the working process of quantitative dilution and quantitative sampling of the sample is realized, excessive contact of the anaerobic microorganisms with oxygen in the environment is avoided, the survival probability of the anaerobic microorganisms is improved, and the practical value is very high.

It is noted that for some implementations, if not essential to the utility model and well known to those of ordinary skill in the art, they are not illustrated in detail in the drawings or in the text of the description, as they may be understood with reference to the relevant prior art.

Further, the foregoing examples are provided merely to enable the utility model to meet the requirements of law, and the utility model may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Further, the above definitions of the various elements and methods are not limited to the various specific structures, shapes or arrangements of parts mentioned in the examples, which may be easily modified or substituted by those of ordinary skill in the art.

It should also be noted that directional terms, such as "upper", "lower", "front", "rear", "left", "right", "inner", "outer", etc., used in the embodiments are only directions referring to the drawings, and are not intended to limit the scope of the present invention. Throughout the drawings, like elements are represented by like or similar reference numerals. Conventional structures or constructions will be omitted when they may obscure the understanding of the present invention.

And the shapes and sizes of the respective components in the drawings do not reflect actual sizes and proportions, but merely illustrate contents of the embodiments of the present invention. Furthermore, in the claims, any reference signs placed between parentheses shall not be construed as limiting the claim.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms can be understood in a specific case to those of ordinary skill in the art.

Furthermore, the word "comprising" does not exclude the presence of elements or steps not listed in a claim. The word "a" or "an" preceding an element does not exclude the presence of a plurality of such elements.

The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention, and it should be understood that the above-mentioned embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A sample dilution tube, comprising:

a tube body;

the sealing piston is freely and slidably sleeved on the inner side of the pipe body;

the sealing cover is arranged on the upper part of the pipe body in a covering manner;

and when a sample is extruded into the diluent in the diluent space, the sealing piston can automatically slide to realize the enlargement of the volume of the diluent space.

2. The sample dilution tube of claim 1, further comprising:

the sampling needle is fixed at the bottom of the sealing piston, a sampling channel is formed in the sampling needle, the sampling channel is upwards communicated with the diluent space, and the needle head of the sampling needle penetrates through the sealing piston and extends to the inner space of the tube body at the lower part of the sealing piston;

and the elastic sealing sleeve is sleeved outside the needle head of the sampling needle.

3. The sample dilution tube of claim 2, wherein:

further comprising: the second filter sheet is arranged at the upper part of the sealing piston and at least covers the opening of the sampling channel facing the diluent space;

further comprising: the bottom cover is detachably covered at the bottom of the tube body, and the sealing piston with the sampling needle is packaged at the inner side of the tube body.

4. The sample dilution tube of claim 3, wherein the second filter has a pore size of between 80 and 400 mesh.

5. The sample dilution tube of claim 1, wherein:

the sealing cover is sunken downwards to form a quantitative cavity which is communicated with the diluent space;

the sample dilution tube further comprises: the middle part of the top cover is downwards protruded to form an extrusion column, and the front end of the extrusion column is matched with the position and the shape of the quantitative cavity;

when the top cover is gradually pressed down, the extrusion column and the quantitative cavity form plunger matching, and a sample in the quantitative cavity is extruded into the diluent.

6. The sample dilution tube of claim 5, wherein the cap is recessed downward outside the dosing chamber to form an overflow chamber.

7. The sample dilution tube of claim 6, wherein the dosing chamber is cylindrical; the overflow chamber is annular and surrounds the periphery of the quantitative cavity.

8. The sample dilution tube of claim 5, wherein;

external threads are formed on the outer peripheral side of the pipe body or the sealing cover;

an internal thread is formed on the inner peripheral side of the cover body of the top cover, and the internal thread is matched with the external thread;

when the quantitative cavity is not used, the bottom of the external thread is provided with a limiting ring, so that the internal thread cannot be screwed to the bottom of the external thread, and the front end of the extrusion column is positioned at the outer side of the quantitative cavity; when the quantitative liquid sample taking device is used, the limiting ring is removed, the extrusion column on the top cover is gradually extruded into the quantitative cavity along with the downward rotation of the meshing of the internal thread and the external thread, and a sample with a corresponding volume is extruded into the diluent space.

9. The sample dilution tube of any one of claims 1-8, wherein:

the tube body and the sealing cover are integrally formed; and/or

The tube body is made of transparent and semitransparent materials; and/or

Further comprising: one or more grinding balls accommodated in the diluent space; and/or

And carrying out deoxidization operation before the diluent is filled, and filling under an anaerobic environment.

10. A sampling kit, comprising:

the sample dilution tube of any one of claims 2 to 4;

the sampling tube is a vacuum tube, the top of the sampling tube is covered with an elastic plug, and the outer diameters of the sampling tube and the elastic plug are smaller than the inner diameter of the tube body of the sample diluting tube;

wherein, during sample transfer, the sampling needle breaks the elastic plug; the elastic plug pushes the sealing piston upwards to slide upwards, so that the diluent containing the sample in the diluent space is squeezed into the sampling tube.