CN106423322A - Pipette tip and pipette system - Google Patents

Pipette tip and pipette system Download PDF

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Publication number
CN106423322A
CN106423322A CN201510870010.5A CN201510870010A CN106423322A CN 106423322 A CN106423322 A CN 106423322A CN 201510870010 A CN201510870010 A CN 201510870010A CN 106423322 A CN106423322 A CN 106423322A
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CN
China
Prior art keywords
capillary tube
sample
pipette tip
adapter
pipette
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201510870010.5A
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Chinese (zh)
Other versions
CN106423322B (en
Inventor
崔义烈
吴茔进
金炳澈
郑景和
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boditech Med Inc
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Boditech Med Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boditech Med Inc filed Critical Boditech Med Inc
Publication of CN106423322A publication Critical patent/CN106423322A/en
Application granted granted Critical
Publication of CN106423322B publication Critical patent/CN106423322B/en
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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022Capillary pipettes, i.e. having very small bore
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/0275Interchangeable or disposable dispensing tips

Abstract

The invention relates to a pipette tip capable of easily achieving quantitative acquisition of samples and a pipette system including the same. The pipette includes a sample acquisition unit (110) which forms a capillary tube (111) in the length direction so as to achieve import of a sample; a cylinder (120) which forms a sample storage chamber (122) for storing the sample, wherein the sample storage chamber communicates with the capillary tube (111) through discontinuous segments (121).

Description

Pipette tip and sucker system
Technical field
The present invention relates to a kind of pipette tip of quantitative collection that can easily realize sample and inclusion The sucker system of this pipette tip, especially, be related to a kind of using capillary tube can quantitative collection relatively The pipette tip of Large Copacity liquor sample, and gather liquor sample using this pipette tip and can enter Row conveying and the sucker system of dispensing.
Background technology
Suction pipe (pipette) refer to measure or use during dispensing liquid by glass or plastics system The thin pipe becoming, has pipette (whole pipet), dropper, Bath according to different purposes Moral suction pipe, automatic pipet(te) etc..
Carry out during clinical immunoassays in order to quantitative collection sample and mixed and dispensing and make With suction pipe (pipette) end.In order to be analyzed testing crew by using with carry out quantitative adopting The pipette tip that matches of capacity of collection is testing.Develop at present and popularized and much can Carry out easily and in a large number the automation equipment of this manual type inspection.
So-called automation equipment refer to by be input to inside program can carry out once with On the Clinical Laboratory equipment automatically processing (dispensing, pretreatment).This automation equipment Shortcoming is that described equipment needs collection to be equivalent to gather the amount of the required sample of measurement The amount sample of the decades of times of required sample.
For a specific example, for check and measure the protein in blood of human body etc. from Dynamicization equipment, needs to check to gather the venous blood of more than 5mL and taken care of one Test in fixed pipe, but as long as be actually used in the amount 10 μ l of the blood of measurement about just Enough, and in automation equipment, only in blood tube, the blood equipped with several mL can be real Existing automatic data collection.10 μ l about blood can be acquired using blood capillary in finger tip and use, And this blood capillary cannot be used in automation equipment, and, for infant and because of blood Pipe is deep and be difficult to the patient taking a blood sample, it will with lead to psychological painful of blood sampling with adopt The difficulty of principal body.
Prior art literature
Patent documentation
Granted patent announces No. 10-1510823 (day for announcing:On April 13rd, 2015)
Content of the invention
(1) technical problem to be solved
It is an object of the invention to solving above-mentioned problems of the prior art, one kind is provided to exist Also it is capable of the pipette tip of a small amount of sample of quantitative collection in the case of not utilizing suction pipe, and can It is readily achieved the suction pipe system of the mixing pretreatment of preprocessing solution and sample using this pipette tip System.
(2) technical scheme
Pipette tip for realizing the present invention of above-mentioned purpose includes:Sample collection unit, along length Degree direction forms capillary tube, is achieved in the importing of sample;And cylinder, it is formed with sample and protect Deposit room, described Storage of sample room is connected with described capillary tube by discontinuous section.
Preferably it is characterised in that the described discontinuous section of inclination angle with capillary tube is 45 ° More than.
Preferably it is characterised in that described capillary tube has the first capillary tube, and section face Long-pending second capillary tube different from described first capillary tube.
Preferably it is characterised in that the cross section of described capillary tube is long hole shape or cross shape.
Secondly, the sucker system of the present invention includes:Pipette tip, it has and is formed along its length Capillary tube and realize the importing of sample sample collection unit, be formed through discontinuous section with The cylinder of the Storage of sample room of described capillary tube connection;And handle apparatus, with described cylinder End is fixed, and can be adjusted in described pipette tip by the pressure source being driven by electric control signal Pressure.
Preferably, described handle apparatus include:Adapter, is formed through the stream being formed, The thus peristome insertion assembling with described cylinder;Pump, can be according to automatically controlled from outside input Signal processed, and the pressure in pipette tip is adjusted by described adapter.
It is highly preferred that described handle apparatus include:Guidance unit, the region being located in pipette tip The movement of described adapter is guided and the region that test kit (cartridage) is located between;Drive Unit, produces the driving force of the position for operating described adapter along described guidance unit;Control Unit processed, produces the electric control signal of the driving controlling described pump and described driver element.
(3) beneficial effect
The pipette tip of the present invention includes the sample collection unit being formed with capillary tube, and is formed with There is the main unit of discontinuous section and the Storage of sample room expanded from capillary tube tip, thus The quantitative collection of sample can be carried out using the capillary tube designing and producing while there is certain capacity, by This has the effect that a small amount of sample for blood capillary etc also can be diagnosed.
The pipette tip of prior art itself does not have the function of can attracting quantitative sample, thus By the way of the volume of the sample that collection is set using the set volume function using suction pipe, and Even if also a constant volume can be carried out in pipette tip itself in the case of not using suction pipe in the present invention The quantitative collection of long-pending liquor sample, is therefore applied in the diagnostic device of automatization, has energy The effect enough automatically being diagnosed using a small amount of sample of blood capillary etc..
And, the sucker system of the present invention is using can carry out the quantitation of sample by capillarity The pipette tip of collection, thus has and can conveniently realize mixing and dispensing with reaction solution Effect.
Brief description
Fig. 1 is the sectional structure chart of the pipette tip of the present invention.
(a) of Fig. 2 is the multiple of the capillary profile of the pipette tip representing the present invention to (f) The figure of embodiment.
Fig. 3 is putting of the sample collection unit of the other embodiment of the pipette tip representing the present invention Big sectional view.
(a) of Fig. 4 is the figure of the using method of pipette tip for the present invention is described to (f).
Fig. 5 is the structure chart of the sucker system of the present invention.
Brief description labelling
100:Pipette tip 110:Sample collection unit
111:Capillary tube 120:Cylinder
121:Section 122:Storage of sample room
310:Adapter 320:Pump
410:Movable framework 411:Vertical ball screw
412:Horizontal ball screw 420:Control unit
500:Transfer device 600:Verification unit
Specific embodiment
The specific structure being proposed in an embodiment of the present invention and functional explanation are only Illustrate that the embodiment of idea of the invention and example, according to the reality of idea of the invention Apply example and can be embodied as various ways.And, the present invention can not be construed to be defined in this specification Embodiment described, and should be understood to be comprised including the thought of the present invention and technical scope All of change thing, equipollent or even substitute.
In addition, though the term such as first and/or second can be used for illustrating multiple groups in the present invention Become component, but described composition component is not limited to described term.Described term is simply to distinguish One forms component to use with the purpose of another composition component, for example, without departing from this In the protection domain of bright concept, the first composition component can be named as the second composition component, is similar to Ground, the second composition component also can be named as the first composition component.
When referring to a composition component with other compositions component " connection " or " joint ", It is possible to be directly connected to other composition components or engage it should be appreciated that being in both Between be also possible to exist other composition components.On the other hand, when refer to one composition component with Other form during component " being directly connected to " or " directly contact " it should be understood that at both There are not other composition components in centre.For illustrate form component between relation "~ Between " and " just~between " or " with~adjacent " and " directly with~adjacent " etc. table State and should also be as similarly explaining.
The term that used in this manual simply to illustrate that specific embodiment and use , it is not used to limit the present invention.Do not conclusively show different meanings within a context On the premise of think of, the representation of odd number includes the representation of plural number." bag in this manual Include " or " having " etc. term it should be appreciated that its be for illustrate implemented feature, Numeral, step, action, the presence of composition component, accessory or these combinations, rather than in advance Exclusion one or more others features or numeral, step, action, composition component, There is a possibility that or additional of accessory or a combination thereof.
The present invention will be described in detail referring to the drawings.
As shown in figure 1, the pipette tip 100 of the present invention includes:Sample collection unit 110, edge Length direction forms capillary tube 111, is achieved in the importing of sample;And cylinder 120, shape Become to have the Storage of sample room 122 that can preserve sample, described Storage of sample room is passed through discontinuous Section 121 is connected with capillary tube 111.
Sample collection unit 110 be may be integrally formed with cylinder 120, and it can be glass or plastics Material, is not limited to this, preferably uses the strong material of rigidity (rigidity), so as not to meeting Produce the change of shape because of the outside power applying.
And, in order to produce effective capillarity and sample can be made using hydrophilic material Sample collection unit 110, or the inner peripheral surface of the capillary tube 111 in sample collection unit 110 The hydrophilic thin film of plating.
Capillary tube 111 is formed there through along the length direction of sample collection unit 110, from there through Preserving sample, the capacity of collection about can use 5 μ l to 100 μ l to capillarity.
Capillary tube can be determined in the range of the collection that can carry out sample by capillarity 111 sizes (diameter) are it is preferable that capillary diameter is about in the model of 0.5mm to 1.5mm Enclose interior decision.
Cylinder 120 can be integrally formed with sample collection unit 110, arranges and capillary tube 111 The Storage of sample room 122 of front end connection, now Storage of sample room 122 is with capillary tube 111 relatively The mode with discontinuous section 121 extends, and thus has the diameter bigger than capillary tube 111.
Discontinuous section 121 inclination angle with capillary tube 111 of example is right angle in FIG, and When tiltangleθ is more than 45 °, it is able to confirm that the liquor sample gathering by capillarity It is filled only with to capillary tube 111, and tiltangleθ is for even more than 90 °.
Storage of sample room 122 is run through the center of cylinder 120 and is formed, and thus can achieve pretreatment The mixing of solution and sample is it is preferable that have cone from discontinuous section 121 along upper direction The inclined plane of shape.
As described above, the peristome being expanded of Storage of sample room 122 upper end can with have right Automation equipment of the taper surface answered etc. formed Lu Ersuo (luer lock) combination it is preferable that Inner peripheral surface in Storage of sample room 122 can form the slot 123 of ring-type, so as to prominent Go out the projection Intercalation being formed on the taper surface of automation equipment.
The pipette tip 100 of the present invention being composed so can be according to the capillary of sample collection unit 110 The volume of pipe 111 is determining correctly to gather capacity.That is, the cross section according to capillary tube 111 Amass and height (L) is to determine the volume of capillary tube 111, gather sample and pass through capillarity Capillary tube 111 below 121 for the filled section.
In addition, the consideration based on collection capacity in the present invention, can be to the section shape of capillary tube Shape carries out various deformation.For example, need correspondingly by sample collection list to increase collection capacity The length (L) of unit makes in long way, but in the present invention for jumbo collection capacity And the size of the cross-sectional area of capillary tube can be increased.
(a), (b), (c) of Fig. 2, (d) are that the capillary tube of the pipette tip representing the present invention cuts open The figure of the various embodiments in face.
With reference to Fig. 2, capillary tube 111a can be circular cross-section (Fig. 2 (a)), or capillary tube 111b can be the hole (Fig. 2 (b)) of elongated hole-shape.And, the capillary tube 111c of the present invention can Being cross-type shape (Fig. 2 (c)), or capillary tube 111d can be on certain radius Overlap has the circular arc of certain angle and the hole (Fig. 2 (d)) that formed.
On the other hand it is preferable that each capillary tube 111a, 111b, 111c, 111d can produce In the range of raw capillarity, its size d (diameter or wide cut) is about scheduled on Between 0.5mm~1.5mm.
Lift another embodiment, Fig. 2 (e) represents there is the capillary tube 111e of circular cross-section It is inserted into the state of plug 112, now, the diameter d1 of capillary tube 111e itself can be set to greatly In embodiment (a) explained above, (b), (c), (d) value, meanwhile can basis Effective dimensions d of the size of plug 112 can produce the size of capillarity Determining in the range of (0.5mm~1.5mm), thus increasing effective area of section, being achieved in Jumbo sample collection.Fig. 2 (f) and be another embodiment, its expression has four sides The state of insertion plug 113 inside the capillary tube 111e of tee section structure.
Fig. 3 is the sample collection unit of another embodiment of the pipette tip representing the present invention Amplification profile.
With reference to Fig. 3, in the pipette tip of the present invention, it is penetratingly formed at sample collection unit 210 Capillary tube 211,212 can be by the first capillary tube 211, Yi Jiyu being formed on front end opening First capillary tube 211 connects and area of section is narrower than 212 groups of second capillary tube of the first capillary tube Become.
On the other hand it is preferable that determining the size (diameter or wide cut) of the second capillary tube 212 In below 1.5mm, capillarity can be produced, it is highly preferred that the first capillary tube 211 It is connected by discontinuous section of 211a with the second capillary tube 212.
As described above, capillary tube by the first capillary zone between A and the second capillary tube interval B group Become, and each interval to be connected by discontinuous section, easily to carry out the great Rong of pipette tip The collection Capacity design of amount design or multiple capacity and making.
Specifically, with reference to Fig. 1, when the overall interval of capillary tube 111 has identical diameter, Jumbo liquor sample collection pipette tip and design capacity proportionally, by capillary tube 111 Length make in long way, so can have in automated analysiss instrument be suitable for pipette tip when need Consider the problems such as produce interference because of the length of pipette tip.
Thus, as shown in figure 3, in the present invention by Large Copacity (50 μ l<) liquor sample adopts Collection pipette tip 200 may be designed to following structure, that is, according to collection capacity by capillary tube 211, 212 are divided into plural interval, and make it have certain length L2's in front end opening Major diameter is interval, thus entire length L1 of capillary tube 211,212 is limited in certain It is capable of required collection capacity while below length.
(a) of Fig. 4 is the figure of the using method of the pipette tip representing the present invention to (f).
As shown in Fig. 4 (a), using pipette tip 100 gather sample 10, now sample 10 because Capillarity and correctly realize quantitative collection in the capillary tube of sample collection unit 110.
As shown in Fig. 4 (b), the pipette tip 100 having been gathered sample 10 installs (mounting) On the handle apparatus of scalable pressure.
Handle apparatus are by being formed through the stream that formed the connection assembling with pipette tip 100 Device 310, and malleation (positive can be produced in pipette tip 100 by adapter 310 Pressure) or the pump 320 of negative pressure (negative pressure) forms, adapter 310 is The pipe of hollow shape, described adapter assembles and passes through pipeline with the top insertion of pipette tip 100 It is connected with pump 320.
Secondly, such as shown in Fig. 4 (c), by the negative pressure being produced by handle apparatus, it is stored in suction The Storage of sample of the sample collection unit 110 of pipe end 100 is to cylinder 120.
As shown in (d), (e) of Fig. 4, will be mobile for the pipette tip 100 being arranged on handle apparatus To preprocessing solution 20, and mixing is manufactured by composite sample 10 and preprocessing solution 20 Liquid 30.Now, the sample 10 of pipette tip 100 is realized by the malleation being produced by pump 320 Discharge.
Secondly, such as shown in Fig. 4 (f), mixed liquor 30 is sucked into pipette tip 100, institute again The mixed liquor 30 sucking can carry out dispensing on desired position.Now, mixed liquor 30 passes through The negative pressure that produced by pump 320 and be sucked into pipette tip 100.
Fig. 5 is the structure chart of the sucker system of the present invention.
As shown in figure 5, the sucker system of the present invention includes:Guidance unit, connects for guiding The shift position of device 310;Driver element, produces for operating adapter along described guidance unit The driving force of 310 position;Control unit, for the driving of controlling pump and driver element.
In the present embodiment, example adapter 310 and the integral part of module type of pump 320, The lower end of pump 320 arrange adapter 310 and together with adapter 310 shift position.Another Aspect, adapter is connected with pump by flexible hose, thus can achieve the position of a moving connector Put to run.
The suction at multiple pipette tips 100 place in quantitative collection liquor sample for the guidance unit Guidance connector 310 between the test kit position at tube end position (site) and test kit 21 place Position move, in the present embodiment the vertical ball screw of example 411, pass through vertical ball spiral shell Bar 411 is come the movable framework 410 to guide and be in vertical motion, and horizontal arrangement is movable On framework 410 and guide pump 320 the motion of horizontal direction horizontal ball screw 412.
In the present embodiment, pipette tip 100 holds in the state of quantitative collection liquor sample It is contained in the frame 401 being arranged in specified location and is configured, test kit 21 is arranged on shifting Send the side of device 500.
Vertical ball screw 411 and horizontal ball screw 412 can be revolved by well-known bearing Turn ground setting, the first drive of the rotation driving of ball screw is provided on each ball screw Dynamic motor 413 and the second drive motor 414.
Control unit 420 controlling pump 320 and the first drive motor 413, the second drive motor 414 Driving, specifically, control unit 420 can by control the first drive motor 413 and second Drive motor 414, to realize the position control of adapter 310, will be adopted by adapter 310 The pipette tip 100 of collection liquor sample moves to test kit 21, thus can be by liquor sample and examination The preprocessing solution of agent box 21 is mixed or dispensing.
On the other hand, pipette tip 100 is moved operation and mixed or dispensing mistake Cheng Zhong, control unit 420 is controlled to pump 320, so that specified pressure can act on In pipette tip 100, liquor sample is thus prevented during movement to flow out, or can be with Preprocessing solution is mixed or dispensing.
Complete to mix with liquor sample or the test kit 21 of dispensing can be moved by transfer device 500 Deliver to verification unit 600 and test.
Illustrate that guidance unit carries out biaxial movement vertically and horizontally in the present embodiment, It is obvious that it should being understood to increase and vertically and horizontally mutual orthogonal The guide member in direction and driving source, are achieved in three axial motions.
The present invention discussed above is not limited to the embodiment above describing and accompanying drawing, not In the range of the technological thought of the present invention, those skilled in the art can carry out various putting Change, deform and change.

Claims (7)

1. a kind of pipette tip, including:
Sample collection unit, forms capillary tube along its length, is achieved in the importing of sample; And
Cylinder, is formed with Storage of sample room, and discontinuous section and institute are passed through in described Storage of sample room State capillary tube connection.
2. pipette tip according to claim 1 is it is characterised in that described discontinuous section It is more than 45 ° with the inclination angle of capillary tube.
3. pipette tip according to claim 1 is it is characterised in that described capillary tube has First capillary tube, and the second capillary tube that area of section is different from described first capillary tube.
4. pipette tip according to claim 1 is it is characterised in that the horizontal stroke of described capillary tube Section is long hole shape or cross shape.
5. a kind of sucker system, including:
Pipette tip, it has the sample forming capillary tube along its length and realizing the importing of sample Collecting unit, it is formed through the discontinuous section of Storage of sample room connecting with described capillary tube Cylinder;And
Handle apparatus, are fixed with the end of described cylinder, can be by being driven by electric control signal Pressure source adjusting the pressure in described pipette tip.
6. sucker system according to claim 5, described handle apparatus include:
Adapter, is formed through the stream being formed, and thus inserts with the peristome of described cylinder Assembling;
Pump, according to the electric control signal from outside input, and can be adjusted by described adapter Pressure in pipette tip.
7. sucker system according to claim 6, described handle apparatus include:
Guidance unit, guides institute between the region that the region that pipette tip is located and test kit are located State the movement of adapter;
Driver element, produces the drive of the position for operating described adapter along described guidance unit Power;
Control unit, produces the electric control letter of the driving controlling described pump and described driver element Number.
CN201510870010.5A 2015-08-04 2015-12-02 Pipette tip and sucker system Active CN106423322B (en)

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Cited By (1)

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CN108246376A (en) * 2018-03-19 2018-07-06 厦门汇川工贸有限公司 A kind of novel quantitative inhales dropper

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ES2878311T3 (en) * 2016-10-28 2021-11-18 Boditechmed Inc Pipette tip and pipette system
KR102572756B1 (en) 2020-11-20 2023-08-30 바디텍메드(주) A socket for for capillary tip desorption
KR102561174B1 (en) * 2023-03-22 2023-07-28 엠비디 주식회사 High-speed dispensing spotter for bio samples

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KR20170017051A (en) 2017-02-15
CN106423322B (en) 2019-10-25

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