CN216718462U - Measuring assembly for full-automatic chemiluminescence immunoassay analyzer - Google Patents

Measuring assembly for full-automatic chemiluminescence immunoassay analyzer Download PDF

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Publication number
CN216718462U
CN216718462U CN202123369464.4U CN202123369464U CN216718462U CN 216718462 U CN216718462 U CN 216718462U CN 202123369464 U CN202123369464 U CN 202123369464U CN 216718462 U CN216718462 U CN 216718462U
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light
assembly
rotating
full
drum
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CN202123369464.4U
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许行尚
杰弗瑞·陈
胡统号
苏灿灿
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Nanjing Lanyu Biological Technology Co Ltd
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Suzhou Aikeruite Biomedical Technology Co ltd
Nanjing Lanyu Biological Technology Co Ltd
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Abstract

The utility model discloses a measuring assembly for a full-automatic chemiluminescence immunoassay analyzer, which comprises a rotary drum, a rotary drum rotating assembly, a liquid transfer upper and lower assembly and a photomultiplier; the rotating drum is provided with at least one reaction cup position, the rotating drum rotating assembly drives the rotating drum to rotate, the liquid-transferring upper and lower assembly drives the liquid-transferring assembly to move up and down to add substrate liquid into the reaction cup or suck away waste liquid after testing, and the photomultiplier is used for measuring a sample to be tested. The measuring assembly for the full-automatic chemiluminescence immunoassay analyzer further comprises a light-proof cavity and a light-proof cover, wherein the light-proof cavity is arranged on the outer side of the rotary drum, and the light-proof cover is arranged at the top of the rotary drum; the photomultiplier with move about the liquid upper and lower subassembly and all establish the outside of light-resistant cavity. The measuring assembly is simple in structure, airtight and light-proof are achieved, interference on measuring results is prevented, and the measuring results are more accurate.

Description

Measuring assembly for full-automatic chemiluminescence immunoassay analyzer
Technical Field
The utility model belongs to the technical field of medical equipment, and particularly relates to a measuring assembly for a full-automatic chemiluminescence immunoassay analyzer.
Background
A chemiluminescence immunoassay analyzer is a medical inspection instrument for performing immunoassay on a human body by detecting serum of a patient. Quantitative patient serum and horseradish peroxidase (HRP) are added into a white opaque microplate coated with the antibody in a solid phase, and molecules to be detected in the serum are specifically combined with a conjugate of the horseradish peroxidase and the antibody on a solid phase carrier. The unreacted free components are separated and washed. And then adding Luminol luminescence base solution, exciting the intermediate by using free energy released by chemical reaction, returning to an excited state from a ground state, and releasing energy in the form of photons. At this time, the microplate is placed into the analyzer, and the photon number of each well is read out sequentially by the photon counter through the three-dimensional transmission system inside the analyzer. And (3) quantitatively analyzing the concentration of the molecules to be detected in the sample according to a mathematical model established by the standard substance. Finally, a data report is printed to assist in clinical diagnosis. Has a plurality of advantages: powerful sample processing system and emergency treatment function; a unique discrete integrated design; complete control, supply system, etc. The measuring process of the chemiluminescence reaction is carried out in a measuring chamber through a high-sensitivity photomultiplier, and the measuring chamber is sealed and light-proof.
SUMMERY OF THE UTILITY MODEL
The technical problem to be solved by the utility model is to provide a measuring component for a full-automatic chemiluminescence immunoassay analyzer, which has a simple structure, realizes sealing and light-proof, prevents interference on a measuring result, and enables the measuring result to be more accurate.
In order to solve the technical problems, the technical scheme adopted by the utility model is that the measuring assembly for the full-automatic chemiluminescence immunoassay analyzer comprises a rotary drum, a rotary drum rotating assembly, a liquid transfer upper assembly and a liquid transfer lower assembly and a photomultiplier; the rotating drum is provided with at least one reaction cup position, the rotating drum rotating assembly drives the rotating drum to rotate, the liquid-transferring upper and lower assembly drives the liquid-transferring assembly to move up and down to add substrate liquid into the reaction cup or suck away waste liquid after testing, and the photomultiplier is used for measuring a sample to be tested. After the technical scheme is adopted, the reaction cup is placed into the inlet and outlet of the reaction cup through the manipulator (namely, in one reaction cup position in the rotary drum), the rotary drum is driven to rotate to the position below the pipetting assembly through the rotary drum rotating assembly, the pipetting assembly is driven to move downwards through the pipetting upper assembly and the pipetting lower assembly to inject substrate liquid into the reaction cup, after the injection is finished, the pipetting upper assembly and the pipetting lower assembly are driven to move upwards, after the measurement of the photomultiplier is finished, the rotary drum is driven to rotate through the rotary drum rotating assembly, waste liquid is sucked away through the pipetting assembly, and finally the rotary drum is driven to rotate through the rotary drum rotating assembly, so that the empty reaction cup is transferred back to the inlet and outlet of the reaction cup.
As a preferred technical scheme of the utility model, the measuring assembly for the full-automatic chemiluminescence immunoassay analyzer further comprises a light-proof cavity and a light-proof cover, wherein the light-proof cavity is arranged at the outer side of the rotary drum, and the light-proof cover is arranged at the top of the rotary drum; the photomultiplier with move about the liquid upper and lower subassembly and all establish the outside of light-resistant cavity. The light-shading cover provided with the light-shading cavity is used for enabling the rotary drum to be subjected to light-shading treatment, and all the parts which are in contact with the outside during measurement need to be subjected to light-shading treatment, so that interference on the measurement result is prevented.
As a preferable technical scheme of the utility model, the liquid transfer assembly comprises a substrate needle and a waste liquid needle, and a light-shielding supplement core and a light-shielding column are arranged on the outer sides of the substrate needle and the waste liquid needle from inside to outside. The substrate needle is used for injecting substrate liquid (buffer liquid) into a reaction cup in the reaction cup position, the waste liquid needle is used for sucking away waste liquid (measured sample) after testing, a controlled injection pump is arranged outside the measuring assembly, the substrate liquid is injected into the reaction cup through the substrate needle for chemiluminescence reaction, after the measurement is independently completed each time, the liquid (waste liquid) after the reaction is automatically sucked out through the waste liquid needle, and then the reaction cup is transferred to an inlet and an outlet of the reaction cup, so that the measurement is completed.
As a preferable technical scheme of the utility model, the upper part of the waste liquid needle is provided with a spring, and the outer side of the spring is provided with a waste liquid needle fixing seat. In order to prevent the waste liquid in the reaction cup from remaining and achieve the effect of dry-wet separation as much as possible, the waste liquid needle is required to be ensured to touch the bottom to suck out all the waste liquid, and therefore, the spring is arranged on the waste liquid needle and can protect the needle from deforming and damaging while the waste liquid needle touches the bottom.
As a preferred technical scheme of the utility model, the pipetting up-down assembly comprises an up-down motor, a screw rod, a fixed connecting block, a sliding rail and a fixed block, wherein the fixed block is connected with the light-shading column, is connected with the sliding block through the fixed connecting block, and is connected with the screw rod; the sliding block is connected with the sliding rail; the upper motor and the lower motor are connected with the sliding block through the screw rod and the connecting block, and the sliding block is driven to move up and down on the sliding rail, so that the light-shading column is driven to move up and down. The upper motor and the lower motor drive the screw rod to drive the fixed connecting block to move, the fixed connecting block drives the sliding block to move up and down along the sliding rail, and meanwhile, the fixed block connected with the fixed connecting block is driven to move up and down along the sliding rail, so that the light-shading column connected with the fixed block is driven to move up and down, namely, the substrate needle and the waste liquid needle are driven to move up and down along the sliding rail.
As the preferred technical scheme of the utility model, the rotary drum rotating component comprises a motor, a driving wheel, a driven wheel, a belt and a rotating shaft; the motor is connected with the driving wheel, the driving wheel is connected with the driven wheel through the belt, the driven wheel is connected with the rotating shaft, the rotating shaft is connected with the rotating drum, the motor drives the driving wheel to move, and the driving wheel drives the driven wheel to move through the belt, so that the rotating drum is driven to rotate. The rotary drum rotating component drives the rotary drum to rotate, so that the reaction cup rotates to the position below the substrate needle, the liquid transfer component injects substrate liquid into the reaction cup to measure, the rotary drum rotating component drives the rotary drum to rotate to enable the reaction cup to rotate to the position below the waste liquid needle, and after the waste liquid needle sucks away all measured waste liquid, the rotary drum rotating component drives the rotary drum to rotate to the inlet and outlet of the reaction cup.
As a preferable technical scheme of the utility model, the bottom of the driven wheel is provided with a coded disc, one side of the coded disc is provided with an optical coupler sensor, and the coded disc and the optical coupler sensor are used for positioning the rotating drum when the rotating drum stops in rotation. The scale is arranged on the coded disc, the optical coupler sensor is arranged on one side of the coded disc, and the rotary drum rotates to stop and position through the coded disc and the optical coupler sensor.
As a preferable technical scheme, a hollow-out part is arranged at the bottom of the rotary drum, and a filling collecting tank is arranged below the hollow-out part. When the substrate needle injects the substrate liquid into the reaction cup, the substrate liquid has the possibility of splashing, so the splashed liquid needs to be discharged, a hollow part is arranged at the bottom of the rotary drum, and the splashed liquid can flow out of the hollow part to the filling collecting tank to be discharged.
As a preferred technical solution of the present invention, the measuring assembly for a full-automatic chemiluminescence immunoassay analyzer further comprises a base, the drum is arranged above the base, and the drum rotating assembly is arranged below the base.
As a preferred technical scheme of the present invention, a measurement light transmission window is arranged on the rotary drum, a lens is arranged between the photomultiplier tube and the rotary drum in the light-shielding cavity, and the lens focuses the point light into parallel light. The photomultiplier measurement needs parallel light to enter the photomultiplier, so a lens is needed to focus point light into parallel light, and the lens is arranged in a light-shading cavity between the photomultiplier and the rotary drum.
Compared with the prior art, this a measuring component for full-automatic chemiluminescence immunoassay appearance is used for full-automatic chemiluminescence immunoassay appearance, and its measurement process is gone on in the measuring chamber through the photomultiplier of high sensitivity, and this measuring component simple structure has realized airtight light-tight, has prevented the interference that causes the measuring result, makes the measuring result more accurate.
Drawings
The following further detailed description of embodiments of the utility model is made with reference to the accompanying drawings:
FIG. 1 is a perspective view of a measuring unit for a full-automatic chemiluminescence immunoassay analyzer according to the present invention;
FIG. 2 is a cross-sectional structural view of a measuring unit for a full-automatic chemiluminescence immunoassay analyzer according to the present invention;
FIG. 3 is a first enlarged view of a portion of the measuring assembly of the present invention for use in a fully automated chemiluminescent immunoassay analyzer;
FIG. 4 is a second enlarged view of a portion of the measuring assembly of the present invention for use in a fully automated chemiluminescent immunoassay analyzer;
FIG. 5 is a partial enlarged sectional structural view of a measuring unit for a full-automatic chemiluminescence immunoassay analyzer according to the present invention;
wherein: 1-a rotating drum; 101-reaction cup position; 102-measuring the light transmissive window; 103-a hollowed-out portion; 2-a pipetting assembly; 201-substrate needle; 202-waste liquid needle; 203-filling the core in dark; 204-light-avoiding columns; 205-a spring; 206-waste liquid needle fixing seat; 3-a photomultiplier tube; 301-a lens; 4-a drum rotation assembly; 401-an electric machine; 402-a driving wheel; 403-driven wheel; 404-a belt; 405-a rotating shaft; 406-code wheel; 407-opto-coupler sensor; 5-pipetting up and down components; 501-up and down motor; 502-lead screw; 503-fixing a connecting block; 504-a slider; 505-a slide rail; 506-fixed block; 6-light-proof cavity; 7-light-proof cover; 8-filling a collecting tank; 9-a base; 901-stent.
Detailed Description
Example (b): as shown in FIGS. 1-5, the measuring assembly for the full-automatic chemiluminescence immunoassay analyzer comprises a rotary drum 1, a rotary drum rotating assembly 4, a liquid transfer assembly 2, a liquid transfer upper and lower assembly 5 and a photomultiplier tube 3; the rotating drum 1 is provided with a reaction cup position 101, the rotating drum rotating component 4 drives the rotating drum 1 to rotate, the pipetting up-down component 5 drives the pipetting component 2 to move up and down to add substrate liquid into the reaction cup or suck away waste liquid after testing, and the photomultiplier 3 is used for measuring a sample to be tested; the measuring assembly for the full-automatic chemiluminescence immunoassay analyzer further comprises a light-proof cavity 6 and a light-proof cover 7, wherein the light-proof cavity 6 is arranged on the outer side of the rotary drum 1, and the light-proof cover 7 is arranged at the top of the rotary drum 1; the photomultiplier tube 3 and the pipetting upper and lower assembly 5 are arranged outside the light-shading cavity 6; the pipetting assembly 2 comprises a substrate needle 201 and a waste liquid needle 202, and the outer sides of the substrate needle 201 and the waste liquid needle 202 are respectively provided with a light-proof supplement core 203 and a light-proof column 204 from inside to outside; wherein the substrate needle 201 is used for injecting substrate liquid (buffer liquid) into the reaction cup in the reaction cup position 101, and the waste liquid needle 202 is used for sucking away waste liquid (measured sample) after the test; a spring 205 is arranged at the upper part of the waste liquid needle 202, and a waste liquid needle fixing seat 206 is arranged at the outer side of the spring 205; in order to prevent the waste liquid in the reaction cup from remaining and achieve the effect of dry-wet separation as much as possible, the waste liquid needle 202 is ensured to touch the bottom to suck out all the waste liquid, therefore, the spring 205 is arranged on the waste liquid needle 202, and the spring 205 can also ensure that the waste liquid needle 202 touches the bottom and can protect the needle from deformation and damage; the pipetting up-down assembly 5 comprises an up-down motor 501, a screw rod 502, a fixed connecting block 503, a slider 504, a slide rail 505 and a fixed block 506, wherein the fixed block 506 is connected with the light-avoiding column 204, the fixed block 506 is connected with the slider 504 through the fixed connecting block 503, the fixed connecting block 503 is simultaneously sleeved on the screw rod 502, the slider 504 is connected with the slide rail 505, and the up-down motor 501 drives the slider 504 to move up and down along the slide rail 505 through the screw rod 502 and the fixed connecting block 503, so as to drive the light-avoiding column 204 to move up and down; the up-and-down motor 501 drives the screw rod 502 to drive the fixed connecting block 503 to move, the fixed connecting block 503 drives the slider 504 to move up and down along the slide rail 505, and simultaneously drives the fixed block 506 connected with the fixed connecting block 503 to move up and down, so as to drive the shading column 204 connected with the fixed block 506 to move up and down, and further drive the substrate needle 201 and the waste liquid needle 202 to move up and down along the slide rail 505; one side of the slide block 504 is provided with a second optical coupling sensor which is used for positioning the pipetting assembly 2 by moving up and down; during measurement, the up-down pipetting assembly 5 drives the light avoiding column 204 to move downwards, so that the substrate needle 201 moves downwards, after substrate liquid is added into the reaction cup, the substrate needle 201 moves upwards through the up-down pipetting assembly 5, the photomultiplier 3 performs measurement, after the measurement is completed, the rotary drum 1 drives the reaction cup to rotate to the position below the waste liquid needle 202, the up-down pipetting assembly 5 drives the waste liquid needle 202 to descend to the bottom of the reaction cup to suck waste liquid (measured sample), and after the waste liquid is completely sucked away, the up-down pipetting assembly 5 drives the waste liquid needle 202 to move upwards; the drum rotating assembly 4 comprises a motor 401, a driving wheel 402, a driven wheel 403, a belt 404 and a rotating shaft 405; the motor 401 is connected with the driving wheel 402, the driving wheel 402 is connected with the driven wheel 403 through the belt 404, the driven wheel 403 is connected with the rotating shaft 405, the rotating shaft 405 is connected with the rotating drum 1, and the rotating shaft 405 is arranged in the center of the rotating drum 1 and penetrates through the rotating drum 1; the motor 401 drives the driving wheel 402 to move, and the driving wheel 402 drives the driven wheel 403 to move through the belt 404, so as to drive the drum 1 to rotate; the rotating drum rotating component 4 drives the rotating drum 1 to rotate, so that the reaction cup is rotated to the position below the substrate needle 201, the pipetting up-down component drives the substrate needle 201 to move downwards, after the substrate liquid is injected into the reaction cup after the substrate liquid is moved in place to measure, the rotating drum rotating component 4 drives the rotating drum 1 to rotate, so that the reaction cup rotates to the position below the waste liquid needle 202, and after the waste liquid needle 202 sucks all measured waste liquid away, the rotating drum rotating component 4 drives the rotating drum 1 to rotate to the inlet and outlet of the reaction cup; a coded disc 406 is arranged at the bottom of the driven wheel 403, an optical coupler sensor 407 is arranged on one side of the coded disc 406, and the coded disc 406 and the optical coupler sensor 407 are used for positioning when the rotary drum 1 stops rotating; scales are arranged on the code disc 406, an optical coupler sensor 407 is arranged on one side of the code disc 406, and the stop and the positioning are realized when the rotary drum 1 rotates through the code disc 406 and the optical coupler sensor 407; a hollow-out part 103 is arranged at the bottom of the rotary drum 1, and a filling collecting groove 8 is arranged below the hollow-out part 103; when the substrate needle 201 injects the substrate liquid into the reaction cup, the substrate liquid may splash, so that the splashed liquid needs to be discharged, and therefore, the bottom of the rotary drum 1 is provided with the hollow part 103, and the splashed liquid can flow out of the hollow part 103 to the filling collection tank 8 and then is discharged; the measuring component for the full-automatic chemiluminescence immunoassay analyzer further comprises a base 9, a support 901 is arranged below the base 9, the rotary drum 1 is arranged above the base 9, and the rotary drum rotating component 4 is arranged below the base 9; a measuring light-transmitting window 102 is arranged on the rotary drum 1, a lens 301 is arranged between the photomultiplier tube 3 and the rotary drum 1 in the distance inside the light-proof cavity 6, and the lens 301 focuses point light into parallel light; since the photomultiplier tube 3 requires parallel light to enter the photomultiplier tube for measurement, the lens 301 is required to focus spot light into parallel light, and therefore the lens 301 is provided in the light-shielding cavity 6 between the photomultiplier tube 3 and the bowl 1.
The working principle is as follows: after a manipulator grabs a reaction cup into an inlet and an outlet of the reaction cup (namely a reaction cup position), the rotary drum rotating component 4 drives the rotary drum 1 to rotate, so that the reaction cup rotates to a position below the substrate needle 201, the pipetting upper and lower components 5 drive the light shielding column 204 to move downwards, so that the substrate needle 201 moves downwards, after substrate liquid is added into the reaction cup, the pipetting upper and lower components 5 drive the substrate needle 201 to move upwards, then the photomultiplier tube 3 provides a light source to perform chemiluminescence measurement, after the measurement is completed, the rotary drum rotating component 4 drives the rotary drum 1 to rotate, so that the reaction cup is driven to a position below the waste liquid needle 202, then the pipetting upper and lower components 5 drive the waste liquid needle 202 to descend to the bottom of the reaction cup to absorb waste liquid (measured sample), after the waste liquid is completely absorbed, the pipetting upper and lower components 5 drive the waste liquid needle 202 to move upwards, the rotary drum rotating component 4 drives the rotary drum 1 to rotate to the inlet and the outlet of the reaction cup, the reaction cup is then removed by the robot and removed from the measuring assembly.
The foregoing shows and describes the general principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the present invention, and that various changes and modifications, such as the number of reaction cups, the size of the bowl, the configuration of the pipetting upper and lower assemblies, etc., may be made without departing from the spirit and scope of the utility model and these changes and modifications are within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims (10)

1. A measuring assembly for a full-automatic chemiluminescence immunoassay analyzer is characterized by comprising a rotary drum, a rotary drum rotating assembly, a liquid transfer upper and lower assembly and a photomultiplier; the rotating drum is provided with at least one reaction cup position, the rotating drum rotating assembly drives the rotating drum to rotate, the liquid transfer upper and lower assembly drives the liquid transfer assembly to move up and down to add substrate liquid or suck away waste liquid after testing into the reaction cup, and the photomultiplier is used for measuring a sample to be tested.
2. The measurement assembly for the full-automatic chemiluminescence immunoassay analyzer according to claim 1, further comprising a light-proof cavity and a light-proof cover, wherein the light-proof cavity is arranged outside the rotary drum, and the light-proof cover is arranged on the top of the rotary drum; the photomultiplier with move about the liquid upper and lower subassembly and all establish the outside of light-resistant cavity.
3. The measurement assembly for the full-automatic chemiluminescence immunoassay analyzer according to claim 2, wherein the pipetting assembly comprises a substrate needle and a waste liquid needle, and the substrate needle and the waste liquid needle are provided with a light-proof supplement core and a light-proof column from inside to outside.
4. The measuring assembly for the full-automatic chemiluminescence immunoassay analyzer of claim 3, wherein the upper part of the waste liquid needle is provided with a spring, and the outer side of the spring is provided with a waste liquid needle fixing seat.
5. The measurement assembly for the full-automatic chemiluminescence immunoassay analyzer according to claim 3, wherein the pipetting up-down assembly comprises an up-down motor, a screw rod, a fixed connecting block, a slider, a slide rail and a fixed block, the fixed block is connected with the light-shielding column, the fixed block is connected with the slider through the fixed connecting block, and the fixed connecting block is connected with the screw rod; the sliding block is connected with the sliding rail; the upper motor and the lower motor are connected with the sliding block through the screw rod and the connecting block, and the sliding block is driven to move up and down on the sliding rail, so that the light-shading columns are driven to move up and down.
6. The measurement assembly for the full-automatic chemiluminescence immunoassay analyzer according to claim 5, wherein the drum rotating assembly comprises a motor, a driving wheel, a driven wheel, a belt and a rotating shaft; the motor is connected with the driving wheel, the driving wheel is connected with the driven wheel through the belt, the driven wheel is connected with the rotating shaft, the rotating shaft is connected with the rotating drum, the motor drives the driving wheel to move, and the driving wheel drives the driven wheel to move through the belt, so that the rotating drum is driven to rotate.
7. The measurement assembly for the full-automatic chemiluminescence immunoassay analyzer of claim 6, wherein the bottom of the driven wheel is provided with a code wheel, one side of the code wheel is provided with a light coupling sensor, and the code wheel and the light coupling sensor are used for positioning the rotating drum when the rotating drum stops rotating.
8. The measurement assembly for the full-automatic chemiluminescence immunoassay analyzer of claim 6, wherein the bottom of the rotary drum is provided with a hollow part, and a perfusion collecting tank is arranged below the hollow part.
9. The measuring assembly for the full-automatic chemiluminescence immunoassay analyzer according to claim 7, further comprising a base, wherein the drum is arranged above the base, and the drum rotating assembly is arranged below the base.
10. The measurement assembly for the full-automatic chemiluminescence immunoassay analyzer of claim 2, wherein the rotating cylinder is provided with a measurement light-transmitting window, and a lens is arranged between the photomultiplier tube and the rotating cylinder in the light-shielding cavity, and the lens focuses the point light into parallel light.
CN202123369464.4U 2021-12-29 2021-12-29 Measuring assembly for full-automatic chemiluminescence immunoassay analyzer Active CN216718462U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202123369464.4U CN216718462U (en) 2021-12-29 2021-12-29 Measuring assembly for full-automatic chemiluminescence immunoassay analyzer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202123369464.4U CN216718462U (en) 2021-12-29 2021-12-29 Measuring assembly for full-automatic chemiluminescence immunoassay analyzer

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CN216718462U true CN216718462U (en) 2022-06-10

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Effective date of registration: 20240605

Address after: 211100 No. 6, Qiande Road, Science Park, Jiangning District, Nanjing, Jiangsu Province

Patentee after: LANSION BIOTECHNOLOGY Co.,Ltd.

Country or region after: China

Address before: 210000 No. 2, Qiande Road, Jiangning District, Nanjing, Jiangsu Province

Patentee before: LANSION BIOTECHNOLOGY Co.,Ltd.

Country or region before: China

Patentee before: Suzhou aikeruite Biomedical Technology Co.,Ltd.