JP2004205268A - Fluorescence immunity measuring method and fluorescence immunity measuring apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a fluorescence immunity measuring method and a fluorescence immunity measuring apparatus for precisely and quickly inspecting the presence or absence and the amount of presence of antigen in a specimen. <P>SOLUTION: The presence or absence or the amount of antigen in the specimen is detected by utilizing a process for bringing an antibody adhering to a rod section into contact with an antigen being present in the specimen of a specimen bottle by dipping the rod section of a waveguide into the container of the specimen bottle being placed rotatably and rotating the specimen bottle. <P>COPYRIGHT: (C)2004,JPO&NCIPI

Description

[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention utilizes an antigen-antibody reaction and an evanescent wave to detect whether or not an antigen is present in a sample, and when present, to detect a suitable amount of a fluorescent immunoassay method and a fluorescent immunoassay method. It relates to a measuring device.
[0002]
[Prior art]
BACKGROUND ART Conventionally, a quantitative method (fluorescent immunoassay) in which a radiolabel (fluorescent label) that emits fluorescent radiation when excited by excitation radiation that increases the energy level is incorporated into an antigen-antibody or a similar complex has been widely known. ing.
[0003]
JP-B-5-21185 has been proposed as one form of a measuring apparatus based on the fluorescent immunoassay. In this publication, a fiber-shaped optical element (wave guide) that transmits excitation light capable of exciting a fluorescent label incorporated as a component of an antigen-antibody complex is used. This optical element can also transmit fluorescence, and the antibody is immobilized on a predetermined area of its surface.
[0004]
The optical element is immersed in the liquid sample to be quantified (in the analyte) so that a predetermined area is completely covered by a layer of a known thickness of the liquid sample, and diffusion to capture the antigen Incubate for longer than necessary for the process to form antigen-antibody complex. The excitation light is emitted to the optical element for a short time to excite the fluorescent dye of the labeled antibody in the complex attached to the optical element, and the fluorescence is measured to detect the antigen.
[0005]
[Problems to be solved by the invention]
The above-mentioned fluorescence immunoassay, when examining the presence or absence and the amount of the antigen in the specimen, there is little contact between the antigen in the liquid sample and the surface of the optical element, and the antibody attached to the surface of the optical element There was a case where accurate measurement could not be performed due to a small chance of contact with the antigen. In addition, it takes a lot of measurement time, and it is difficult to accurately inspect many samples in a short time.
[0006]
[Means for Solving the Problems]
An object of the present invention is to provide a fluorescence immunoassay method and a fluorescence immunoassay apparatus that can accurately and quickly examine the presence or absence and the amount of an antigen in a sample.
In order to achieve this object, in the fluorescence immunoassay using the above-mentioned wave guide, the following invention was achieved in which a turntable for handling a large number of samples and a rotation for stirring in each sample bottle were mechanically combined. .
[0007]
The fluorescence immunoassay of the invention according to claim 1 is
The rod portion of the wave guide is immersed in the sample in the sample bottle, and the sample bottle is rotated to contact the antibody attached to the rod portion with the antigen present in the sample. A fluorescent immunoassay device for detecting the presence or absence and / or the amount of the antigen using a step of contacting the fluorescent labeled antibody with
A rotatable sample bottle holding plate provided with a plurality of sample bottle mounting sections for rotatably mounting a sample bottle along a circumferential method,
A wave guide holder plate that can rotate coaxially with a rotation axis of a sample bottle holding plate that holds a wave guide holder provided for each sample bottle,
A sample bottle rotation table coaxial with the rotation axis of the sample bottle holding plate,
Driving means for rotating the sample bottle holding plate and the sample bottle rotating table so that a difference occurs between the two speeds,
A rotation mechanism for rotating and revolving the sample bottle by the speed difference,
A handling arm for moving the wave guide to a predetermined position,
It is characterized by having.
[0008]
The invention of claim 2 is the invention according to claim 1,
The rotation mechanism section includes a gear section provided on the rotary table, and a gear section provided on a sample bottle mounting section that meshes with the gear section.
[0009]
The invention according to claim 3 is the invention according to claim 1,
A cleaning port for cleaning the wave guide;
A disposal box for discarding the wave guide after measurement,
It is characterized by having.
[0010]
The fluorescent immunoassay method of the invention according to claim 4 comprises:
Step 1 of rotating the turntable to move the sample bottle to a predetermined position;
Step 2 of holding the wave guide with the handling arm and moving it to a predetermined position;
Washing the wave guide at the washing port, step 3,
Step 4, in which light is incident on the wave guide and the reference light not derived from the antigen-fluorescence-labeled antibody complex is measured,
Step 5, dipping the wave guide into the sample in the sample bottle,
Dipping the wave guide in a solution containing a fluorescently labeled antibody,
Step 7, in which light is incident on the wave guide and the emitted fluorescence is measured,
Step 8 of calculating the amount of antigen from the respective light amounts obtained in steps 4 and 7;
Step 9, displaying the result
(In any order).
[0011]
The wave guide holder of the invention of claim 5 is
A wave guide holder used for fluorescence immunoassay,
Labeled antibody storage room,
Wave guide storage room,
A sample contact portion suspended to immerse the wave guide in the sample,
Is provided.
According to a sixth aspect of the present invention, in the fifth aspect of the present invention, the widths of both ends of the upper portion of the wave guide holder are different.
[0012]
The invention of claim 7 is the invention of claim 5,
When the heights of the portions supporting the specific position of the wave guide in the labeled antibody storage room, the wave guide storage room, and the sample contact portion are Db, Da, and Dc, respectively,
Db>Da> Dc
It is characterized by satisfying.
According to an eighth aspect of the present invention, a wave guide and a labeled antibody are stored in the wave guide holder according to the fifth aspect, and a sample bottle is combined.
[0013]
The invention of claim 9 is the invention of claim 8,
A seal member covering at least the labeled antibody storage chamber and the wave guide storage chamber is provided above the stage.
[0014]
BEST MODE FOR CARRYING OUT THE INVENTION
Hereinafter, a preferred embodiment of the present invention will be described with reference to the drawings. The materials used and the detailed shape of each part of the inspection machine main body are not limited to these.
[0015]
1 and 2, reference numeral 1 denotes an inspection kit (to be described later with reference to FIG. 6), which is used by being attached to the inspection machine main body 2.
[0016]
The inspection machine main body 2 includes a turntable 11 (which comprises a wave guide holder plate, a sample bottle holding plate, and a sample bottle rotating table, which will be described later), a YZ handling arm unit 12, a washing port unit 14, an optical measurement unit 13, a wave guide disposal box. 15, an electrical box 7 including signal processing means of each name, various driving means M1 to M6, and the like.
[0017]
The turntable 11 has a rotating shaft 110 for revolving the sample bottle 43 and holds the sample bottle 43. The YZ handling arm unit 12 moves the wave guide 41 according to the measurement flow.
[0018]
Reference numeral 3 denotes a personal computer (hereinafter, referred to as a personal computer), which performs various types of arithmetic processing by software.
[0019]
Reference numeral 5 denotes a cleaning liquid bottle, which supplies the cleaning liquid to the cleaning port section 14 using an air supply hose 5a and a water supply hose 5b.
[0020]
Reference numeral 6 denotes a waste liquid bottle which discharges and holds the cleaning liquid in the cleaning port section 14 through a hose 6a. Reference numeral 16 denotes a cleaning suction unit.
[0021]
16a is a suction pump for sucking and holding the wave guide by the wave guide suction section 16e, and 16b is a liquid sending pump for the hose 5b. Reference numeral 16c is a liquid sending valve. 16d is a wave guide suction system valve / sensor.
[0022]
FIG. 3 illustrates a main block of a measurement system in the fluorescence immunoassay apparatus of the present invention.
[0023]
In FIG. 3, reference numeral 3 denotes a personal computer (control means), which receives various results of the fluorescence immunoassay from the signal processing means 7a and various operation instruction programs for giving operation instructions to the operation control / power supply means 7b, and processes the results. It has a processing program and the like.
[0024]
The operation control / power supply unit 7b transmits and receives operation signals to and from the optical measurement unit 13, the cleaning suction unit 16, the driving unit M, and the like in accordance with an operation instruction from the personal computer 3.
[0025]
The optical measuring unit 13 has a laser diode, a photodiode, and the like for measuring fluorescence immunity with respect to the waveguide 41.
[0026]
The cleaning / suction means 16 includes a wave guide suction pump / sensor 16a, a cleaning liquid feed pump 16b for cleaning the wave guide, and the like.
[0027]
The driving means M includes a turntable driving motor / sensor M1, a detection bottle rotating motor M2, a washing port X-axis driving motor / sensor M3, a washing port Z-axis driving motor / sensor M4, a handle arm Y-axis driving motor / sensor M5, and a handle. It has an arm Z-axis drive motor / sensor M6 and the like.
[0028]
FIG. 4 shows the outline of the inside of the optical measurement unit 13 and the concept of the antigen-antibody reaction that occurs on the surface of the rod 413 of the wave guide 41 constituting a part of the test kit in this embodiment.
[0029]
When the antibody 33 is fixed to the surface of the rod portion 413 of the wave guide 41 by coating and immersed in the sample in the sample bottle 43, if the target antigen 34 is present in the sample, the antigen 34 binds to the antibody 33. I do. The fluorescent labeled antibody 35 is further bound to the captured antigen 34.
[0030]
Then, the laser beam 37 from the laser diode LD as excitation light is made to enter the rod portion 413 from the lens portion 36 on the light incident side of the waveguide 41 via the mirror 134. When the laser beam 37 is totally reflected on the surface of the rod 413 and guided, an evanescent wave 38 is generated at the reflection interface of the rod 413. When the evanescent wave 38 generated at this time is incident on the labeled antibody 35, fluorescence 39 is generated from the labeled antibody 35. The generated fluorescent light 39 enters the rod portion 413, follows the rod portion 413 while being totally reflected, exits from the lens portion 36, passes through the filter 135 for cutting noise light, and passes through the lens 136. The light is collected and detected by a photodetector (photodiode) 137.
[0031]
Thus, whether or not the target antigen 34 is present in the sample and the amount of the antigen 34 are detected. Reference numeral 415 denotes an excitation light absorber provided at the end of the rod portion 413 for absorbing excess laser light.
[0032]
FIG. 5 is an example of an operation flow of the inspection system of the present embodiment.
[0033]
The user starts the system and starts the inspection (step S1). The sample bottle 43 constituting the test kit 1 is set on the sample bottle mounting portion of the turntable 11 (Step S2). The user injects the sample into the sample bottle 43 (Step S3). Step S2 and step S3 may be reversed. Inspection information is input from the personal computer 3 (step S4), and a measurement start instruction is input (step S5).
[0034]
The following is done automatically by the system. The designated kit is moved below the YZ handling arm 12 by the rotation of the turntable 11 (step S6). The wave guide 41 housed in the wave guide storage chamber 421 of the wave guide holder 42 of the test kit 1 is taken out by the wave guide handling section 121 of the YZ handling arm section 12 and immersed in the sample in the sample bottle 43 (step S7). . The rotation of the sample bottle 43 gives a relative flow of the sample to the wave guide 41 (step S8).
[0035]
The wave guide 41 is taken out of the sample bottle 43 by the wave guide handling unit 121 (FIG. 13), moved to the washing port unit 14, and then the washing port unit 14 is connected to the optical measuring unit 13 by the XZ driving unit. The wave guide 41 is washed (Step S9). While the waveguide 41 is immersed in the cleaning liquid, optical measurement is performed by the optical measurement unit 13 to obtain a reference signal (step S10). This continuous step of steps S9 and S10 may be performed before step S6.
[0036]
Next, the wave guide 41 in the cleaning unit 14 is taken out by the wave guide handling unit 121, immersed in the labeled antibody storage chamber 422 of the wave guide holder 42, and the labeled antibody stored therein is attached (step S11).
[0037]
Next, the wave guide 41 is taken out by the wave guide handling unit 121, moved to the cleaning unit 14, and the wave guide 41 is cleaned (step S12). Then, the cleaning unit 14 is moved in the direction of the optical measurement unit 13 by the XZ driving unit, and the wave guide 41 is optically measured by the optical measurement unit 13 to obtain a fluorescence signal (step S13).
[0038]
The amount of antigen is calculated from the detection results of steps S10 and S13 (step S14). Next, the inspection result is displayed on the display means 7 such as a display (step S15). The wave guide 41 used for the inspection is discarded in the wave guide disposal box 15 by the wave guide handling unit 121 (step S16), and the inspection ends (step S17).
[0039]
FIG. 6 is a schematic diagram (cross-sectional view) of a main part of the test kit 1 of the present embodiment.
[0040]
The test kit 1 of the present embodiment has a wave guide 41, a wave guide holder 42, a sample bottle 43, and, as a more desirable form, a seal member 44.
FIG. 6 shows an initial state of the test kit 1 and shows a state in which the wave guide 41 is housed in the wave guide storage chamber 421.
[0041]
FIG. 7 is a schematic external view of an example of the wave guide 41.
[0042]
In FIG. 7, a wave guide 41 has a lens portion 411 for efficiently guiding fluorescence to the optical measurement portion 13 of the main body, a cone portion 412 for efficiently introducing laser light, and a rod for providing a surface for performing an antigen-antibody reaction. Part 413, a transparent molded product made of polystyrene composed of four structures of a flange portion 414 for positioning and holding the wave guide, and excitation applied with a member (black paint) for absorbing excess laser light A light absorber 415 is provided.
[0043]
FIG. 8 is a schematic view of a main part of the wave guide holder 42 of the present embodiment. FIG. 8A is a plan view, and FIG. 8B is a side view.
[0044]
The wave guide holder 42 has a cylindrical wave guide storage chamber 421 for protecting and storing the wave guide 41 before use, a cylindrical labeled antibody storage chamber 422 for storing the labeled antibody and immersing the wave guide, and a support for these. 423. Both storage chambers also have a function of positioning the wave guide holder 42 on the turntable 11.
[0045]
The support 423 has an opening 423-a1 and a concave stage 423-a for the wave guide storage chamber 421, and an opening 423-b1 and a stage 423-b for the labeled antibody storage chamber 422 (in the upper surface of the support 423). And an opening 423-c1 for suspending the wave guide 41 with respect to the sample bottle 43 and a concave stage 423-c. Each stage supports the flange portion of the wave guide during storage and immersion. In particular, the concave shapes 423-a and 423-c also have a positioning function with respect to the main body.
[0046]
The three stages 423-a, 423-b, 423-c and the three openings 423-a1, 423-b1, 423-c1 are linearly arranged when viewed from above. As shown in FIG. 6, the height of the three stages 423a, 423b, and 423c, that is, the height of the portion supporting the flange portion of the wave guide 41,
Dc <Db <Da
It has become. This positional relationship is useful when sealing the upper part of each storage chamber and for ensuring a sufficient immersion length of the waveguide with respect to the sample.
[0047]
When the wave guide holder 42 is placed on the turntable 11, the stage 423-c is located at the outermost periphery, the stage 423-a is located at the center, and the stage 423-b is located at the innermost periphery. In order to arrange a plurality of test kits on a circular turntable in the circumferential direction, the circumferential width of the support 423 on the stage 423-b side is smaller than the width on the stage 423-c side.
The seal member 44 is provided for the purpose of transporting the test kit 1, outflow of the labeled antibody during handling, dropping of the wave guide 41 from the wave guide holder 42, and protection. An aluminum layer for protecting the waveguide 41 and the labeled antibody from contamination by outside air and light, or an OPP layer that can be easily welded to the waveguide holder 42 can be used.
[0048]
An example of the sample bottle 43 in the present embodiment is shown in FIG.
A sample storage section 431, a projection 430 having a space 430a therein, and a guide shape section (top surface) 432 serving as a visual guide for the amount of sample to be inserted are provided at a central portion on the inner side of the sample bottle 43. However, the shape of the sample bottle is not limited to this.
FIG. 15 summarizes how each part of the test kit 1 described above functions in the operation flow (FIG. 5) of the test system of the present embodiment.
[0049]
Next, each member of the inspection machine main body 2 will be described.
[0050]
FIG. 10 is a perspective view of a main part of the turntable 11 of the present embodiment. FIG. 11 is an explanatory diagram of a part of FIG.
[0051]
The turntable 11 mainly includes a plurality of components described below.
[0052]
140 is a holder holding plate that holds the wave guide holder 42, 141 is a sample bottle holding plate that holds the sample bottle 43, 142 is a sample bottle mounting portion holding plate, and 143 is the sample bottle 43 via the sample bottle mounting portion 113. 5 is a sample bottle rotation table for rotating the sample bottle. A lift prevention plate for the wave guide holder 42 may be provided above the turntable 11.
[0053]
The wave guide holder 42 is held by using the two storage portions 421 and 422 of the holder and the openings 114 and 115 for receiving the two.
The position detection in the rotation direction of the turntable 11 is performed by an encoder plate and a sensor provided below the turntable.
[0054]
FIG. 12 is a schematic diagram of a sample bottle driving mechanism that is a lower layer of the turntable.
[0055]
The sample bottle mounting section 113 has a sample bottle rotation shaft 144 and a gear 145.
[0056]
The sample bottle rotation table 143 is driven to rotate by the driving force from the driving unit M2 via the pulley 2. The sample bottle mounting portion holding plate 142 is rotationally driven by the driving force from the driving unit M1 via the pulley 1.
[0057]
The gear 148 provided on the inner surface of the sample bottle rotating table 143 and the gear 145 provided below the sample bottle mounting portion are engaged with each other, and transmit the rotational driving force to the sample bottle 43 via the coupling 146 (FIG. 11). are doing.
[0058]
In the above structure, when a rotation speed difference occurs between the sample bottle rotation table 143 and the sample bottle mounting portion holding plate 142, the gear 145 is rotationally driven via the gear 148 provided on the inner surface of the sample bottle rotation table 143. Is done. Thus, the sample bottle 43 is rotated and revolved.
[0059]
FIG. 13 is a perspective view of a main part of the YZ handling arm unit 12.
[0060]
The YZ handling arm unit 12 is an arm for transferring the wave guide 41 from the storage stage 423-a to the sample contact stage 423-c, the labeled antibody attachment stage 423-b, the washing / measurement port 14, and the wave guide disposal box 15. It consists of a mechanism.
[0061]
Driving of the YZ handling arm unit 12 in the YZ direction is performed by a combination of independent motors, timing belts, and pulleys. Reference numeral 123 denotes a Y-direction drive unit that moves in the Y direction, and reference numeral 124 denotes a Z-direction drive unit that moves in the Z direction. The position detection in the YZ direction of each of the drive units 123 and 124 is performed by an encoder and a sensor, respectively.
[0062]
Handling of the wave guide 41 is performed by suction (air suction) in the wave guide handling section 121, and the flange-shaped portion of the wave guide 41 is held by a rubber pad. In front of the wave guide handling section 121, there is provided a needle section 122 for opening the seal of the labeled antibody storage section. The suction presence / absence detection of the wave guide 41 is performed by a negative pressure sensor.
[0063]
FIG. 14 is a schematic view of a main part of the cleaning port unit 14 for cleaning the waveguide and the optical measuring unit 13.
[0064]
The cleaning port section 14 has a holding section 131 that is inserted and held by the wave guide 41 and serves as a cleaning container, a moving mechanism in the XZ direction thereof, and a water guide channel and a drain channel of the cleaning liquid connected to the holding section 131. . The holding unit 131 is connected to the optical measurement unit 13 by moving in the X and Z directions with the waveguide 41 inserted, and the optical measurement unit 13 detects fluorescence or reference light from the waveguide 41. The holding portion 131 receives the flange-shaped portion 414 of the wave guide 41 from the outer periphery and positions the wave guide 41. A rubber O-ring or gasket is provided between the holding unit 131 and the wave guide 41, and the structure is such that when the holding unit 131 collides with a part of the optical measurement unit 13 by the movement in the Z direction, the sealed product is maintained. Has become.
[0065]
Movement in the X direction is performed by a motor, a timing belt, and a pulley, and movement in the Z direction is performed by a motor and a screw mechanism. Position detection in both directions is performed by encoders and sensors.
The inside of the optical measurement unit 13 is as described above with reference to FIG.
[0066]
The wave guide 41 whose measurement has been completed is forcibly discarded in the wave guide discard box 15 in order to prevent the user from reusing or re-measuring by mistake. The wave guide disposal box 15 has a detachable structure to facilitate carrying out accumulated waste.
[0067]
【The invention's effect】
According to the present invention, it is possible to achieve a fluorescence immunoassay method and a fluorescence immunoassay apparatus capable of accurately and rapidly examining the presence / absence and amount of an antigen in a sample.
[Brief description of the drawings]
FIG. 1 is an external view of a fluorescent immunoassay apparatus of the present invention. FIG. 2 is an explanatory view of a main body of an inspection apparatus in the fluorescent immunoassay apparatus of the present invention. FIG. 3 is a block diagram of a main part of the fluorescent immunoassay apparatus of the present invention. FIG. 4 is an explanatory view of an optical measuring section of the immunofluorescence measuring apparatus of the present invention and an antigen-antibody reaction used in the apparatus. FIG. 5 is a flowchart of an example of a test in the fluorescent immunoassay method of the present invention. FIG. Schematic diagram of the kit [Fig. 7] Schematic appearance of a wave guide used in the fluorescence immunoassay device of the present invention [Fig. 8] Explanatory diagram of the wave guide holder of the present invention [Fig. 9] Sample used in the fluorescence immunoassay device of the present invention FIG. 10 is an explanatory view showing an example of a bottle. FIG. 10 is an explanatory view of a turntable in the fluorescent immunoassay apparatus of the present invention. FIG. 11 is an explanatory view of a lower layer of the turntable in the fluorescent immunoassay apparatus of the present invention. FIG. 13 is an explanatory diagram of a sample bottle driving system of a turntable in the photoimmunoassay device. FIG. 13 is an explanatory diagram of a YZ handling unit in the fluorescence immunoassay device of the present invention. FIG. FIG. 15 is an explanatory view showing the function of the test kit of the present invention.
DESCRIPTION OF SYMBOLS 1 Inspection kit 2 Inspection machine main body 3 Personal computer 5 Cleaning liquid bottle 6 Waste liquid bottle 7 I / O port 11 Turntable 12 YZ handling arm part 13 Optical measurement part 14 Cleaning port part 15 Wave guide disposal box 16 Cleaning suction means 41 Wave guide 42 Wave guide holder 43 Sample bottle 113 Sample bottle mounting part 121 Wave guide handling part 122 Needle part 123 Y direction drive part 124 Z direction drive part 131 Holding part (wash port part)
140 Wave guide holding plate 141 Sample bottle holding plate 142 Sample bottle mounting part holding plate 143 Sample bottle rotating table 145, 148 Gear 146 Coupling

Claims (9)

The rod part of the wave guide is immersed in the sample in the sample bottle, and the antibody fixed to the rod part is brought into contact with the antigen present in the sample by rotating the sample bottle, and subsequently, the antigen and A fluorescent immunoassay device for detecting the presence or absence and / or the amount of the antigen using a step of contacting with a fluorescently labeled antibody,
A rotatable sample bottle holding plate provided with a plurality of sample bottle mounting portions for rotatably mounting a sample bottle along a circumference,
Holding a waveguide guide holder provided for each sample bottle, a wave guide holder plate that can rotate coaxially with the rotation axis of the sample bottle holding plate,
A sample bottle rotation table coaxial with the rotation axis of the sample bottle holding plate,
Driving means for rotating the sample bottle holding plate and the sample bottle rotating table so that a difference occurs between the two speeds,
A rotation mechanism for rotating and revolving the sample bottle by the speed difference,
A handling arm for moving the wave guide to a predetermined position,
A fluorescent immunoassay device comprising: 2. The fluorescence according to claim 1, wherein the rotation mechanism unit includes a gear unit provided on the rotary table and a gear unit provided on a sample bottle mounting unit that meshes with the gear unit. 3. Immunoassay device. Cleaning port for cleaning the wave guide,
A waste box that discards the wave guide after measurement,
The fluorescence immunoassay according to claim 1, comprising: Step 1 of rotating the turntable to move the sample bottle to a predetermined position;
Step 2 of holding the wave guide with the handling arm and moving it to a predetermined position;
Washing the wave guide at the washing port, step 3,
Step 4, in which light is incident on the wave guide and the reference light not derived from the antigen-fluorescence-labeled antibody complex is measured,
Step 5, dipping the wave guide into the sample in the sample bottle,
Dipping the wave guide in a solution containing a fluorescently labeled antibody,
Step 7, in which light is incident on the wave guide and the fluorescence derived from the antigen-fluorescence-labeled antibody complex is measured,
Step 8 of calculating the amount of antigen from the respective light amounts obtained in steps 4 and 7;
Step 9, displaying the result
A fluorescent immunoassay method comprising: A wave guide holder used for fluorescence immunoassay,
Labeled antibody storage room,
Wave guide storage room,
A sample contact portion suspended to immerse the wave guide in the sample,
A wave guide holder, characterized by being provided with: The wave guide holder according to claim 5, wherein the widths of both ends of the upper portion are different. When the heights of the portions supporting the specific position of the wave guide in the labeled antibody storage room, the wave guide storage room, and the sample contact portion are Db, Da, and Dc, respectively,
Db>Da> Dc
The wave guide holder according to claim 5, wherein the following conditions are satisfied. A test kit comprising a wave guide and a labeled antibody stored in the wave guide holder according to claim 5, and a sample bottle in combination. The test kit according to claim 8, further comprising a seal member that covers at least the labeled antibody storage chamber and the wave guide storage chamber above the waveguide guide holder.

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