CN216550386U - Stem cell preparation device - Google Patents
Stem cell preparation device Download PDFInfo
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- CN216550386U CN216550386U CN202123013110.6U CN202123013110U CN216550386U CN 216550386 U CN216550386 U CN 216550386U CN 202123013110 U CN202123013110 U CN 202123013110U CN 216550386 U CN216550386 U CN 216550386U
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- stem cell
- cell culture
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- culture barrel
- liquid injection
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Abstract
The utility model relates to the technical field of stem cells, in particular to a stem cell preparation device, which comprises a stem cell culture barrel, wherein a culture barrel cover is arranged above the stem cell culture barrel, a liquid injection pipe is arranged inside the culture barrel cover, a liquid injection control valve is arranged on the left side of the liquid injection pipe, a communicating pipe is fixedly arranged on the right side of the liquid injection pipe, and a gas buffer balloon is connected above the communicating pipe; and the nutrient solution drain pipe is arranged below the stem cell culture barrel, and a drain valve is arranged on the left side of the nutrient solution drain pipe. This stem cell preparation device can avoid external air to enter into the inside of stem cell culture bucket through the nutrient solution fluid-discharge tube flowing back that is the S type to gaseous buffering balloon is saved the inside gas of stem cell culture bucket, plays a cushioning effect, takes place the air exchange with the external world when avoiding changing the culture solution, and the inside that leads to stem cell culture bucket receives the pollution, and then influences the preparation of stem cell preparation.
Description
Technical Field
The utility model relates to the technical field of stem cells, in particular to a stem cell preparation device.
Background
In adult organs, stem cells can repair tissues by constantly dividing, or are in a static state like in mammalian brain tissues, can expand their number by symmetrically dividing during their development, or self-renew and generate more progenitor cells of different differentiation types by asymmetrically dividing, and have a very wide application prospect in the fields of cell repair in life sciences, developmental biology, pharmacology and the like.
When the conventional stem cell preparation device is used for culturing stem cells and replacing culture solution, the culture solution and stem cell tissues can be polluted by contacting with the outside, so that stem cell culture failure is easily caused.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a stem cell preparation device, which aims to solve the problem that when the culture solution is replaced in the stem cell culture process, the culture solution and stem cell tissues are polluted by contacting with the outside, so that the stem cell culture fails easily.
In order to achieve the purpose, the utility model provides the following technical scheme: a stem cell preparation device comprising:
the stem cell culture barrel is characterized in that a culture barrel cover is arranged above the stem cell culture barrel, a liquid injection pipe is arranged inside the culture barrel cover, a liquid injection control valve is arranged on the left side of the liquid injection pipe, a communicating pipe is fixedly arranged on the right side of the liquid injection pipe, and a gas buffer balloon is connected above the communicating pipe;
and the nutrient solution drain pipe is arranged below the stem cell culture barrel, a drain valve is arranged on the left side of the nutrient solution drain pipe, and a bolt is arranged below the nutrient solution drain pipe.
Preferably, the liquid injection pipe penetrates through the inside of the culture barrel cover, the communicating pipe penetrates through the inside of the culture barrel cover, and the gas buffering balloon is communicated with the stem cell culture barrel through the communicating pipe.
Preferably, the nutrient solution drain pipe is communicated with the stem cell culture barrel and is of an S-shaped structure.
Preferably, the stem cell culture bucket is further provided with:
the stem cell culture barrel is arranged in the stem cell culture barrel, a heating rod is arranged in the heating water tank, and the heating water tank is of an annular structure.
Preferably, the culture barrel cover is further provided with:
the stirring motor is arranged above the culture barrel cover, the output end of the stirring motor is provided with a transmission rod, stirring rods are fixedly arranged on two sides of the transmission rod, the transmission rod penetrates through the interior of the culture barrel cover, and the transmission rod is movably connected with the culture barrel cover.
Preferably, the stem cell culture bucket is further provided with:
spacing spout, it is seted up the inner wall of stem cell culture bucket, the internally mounted of spacing spout has spacing slider, spacing slider is kept away from one side of spacing spout and is installed the stem cell and hold the ware, the internally mounted that the stem cell held the ware has the filter screen, the top fixed mounting that the stem cell held the ware has the action bars.
Preferably, the shape and size of the stem cell containing dish are matched with the shape and size of the inside of the stem cell culture barrel, and the stem cell containing dish slides inside the stem cell culture barrel through a limiting sliding groove and a limiting sliding block.
Compared with the prior art, the utility model provides a stem cell preparation device, which has the following beneficial effects: this stem cell preparation device can avoid external air to enter into the inside of stem cell culture bucket through the nutrient solution fluid-discharge tube flowing back that is the S type to gaseous buffering balloon is saved the inside gas of stem cell culture bucket, plays a cushioning effect, takes place the air exchange with the external world when avoiding changing the culture solution, and the inside that leads to stem cell culture bucket receives the pollution, and then influences the preparation of stem cell preparation.
1. According to the utility model, when the S-shaped nutrient solution drainage pipe drains liquid, external air can be prevented from entering the interior of the stem cell culture barrel, and the gas buffer balloon stores the gas in the stem cell culture barrel, so that a buffer effect is achieved, and the phenomenon that the interior of the stem cell culture barrel is polluted due to air exchange with the outside when the culture solution is replaced, and further the preparation of a stem cell preparation is influenced is avoided;
2. according to the utility model, water in the heating water tank is heated through the heating rod, and then the culture solution in the stem cell culture barrel is heated, so that the culture solution is uniformly heated, the phenomenon that the culture solution is locally overheated and the culture of stem cells is influenced is avoided, meanwhile, the culture solution in the stem cell culture barrel is uninterruptedly stirred through the transmission rod, the stirring rod and the stirring motor, the workload of manual liquid mixing is reduced, and meanwhile, the heating rod and the heating water tank are matched for better heating the culture solution;
3. the stem cell tissue can be taken out of the culture solution through the stem cell containing vessel and the filter screen, so that the separation operation of the stem cell tissue and the culture solution becomes simple and convenient, and the workload of the separation of the stem cell tissue of workers is reduced.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1A;
FIG. 3 is a schematic view of the external structure of the nutrient solution drain pipe according to the present invention;
FIG. 4 is a schematic view showing a connection structure between a stem cell culture bucket and a stem cell containing dish according to the present invention;
FIG. 5 is an enlarged view of a portion of the structure shown at B in FIG. 4 according to the present invention.
In the figure: 1. a stem cell culture bucket; 2. a transmission rod; 3. a stirring rod; 4. an operating lever; 5. a stem cell holding dish; 6. a nutrient solution drain pipe; 7. a filter screen; 8. a communicating pipe; 9. a stirring motor; 10. a gas buffer balloon; 11. a liquid injection pipe; 12. a liquid injection control valve; 13. cultivating the barrel cover; 14. a heating rod; 15. a heating water tank; 16. a drain valve; 17. a bolt; 18. a limiting chute; 19. and a limiting sliding block.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 and 3, a stem cell preparation apparatus includes: a stem cell culture barrel 1, wherein a culture barrel cover 13 is arranged above the stem cell culture barrel 1, a liquid injection pipe 11 is arranged inside the culture barrel cover 13, a liquid injection control valve 12 is arranged on the left side of the liquid injection pipe 11, a communicating pipe 8 is fixedly arranged on the right side of the liquid injection pipe 11, and a gas buffer balloon 10 is connected above the communicating pipe 8; the liquid injection pipe 11 penetrates through the interior of the culture barrel cover 13, the communicating pipe 8 penetrates through the interior of the culture barrel cover 13, and the gas buffering balloon 10 is communicated with the stem cell culture barrel 1 through the communicating pipe 8; a nutrient solution drain pipe 6 installed below the stem cell culture tank 1; the nutrient solution drain pipe 6 is communicated with the stem cell culture barrel 1, the nutrient solution drain pipe 6 is of an S-shaped structure, a drain valve 16 is arranged on the left side of the nutrient solution drain pipe 6, and a bolt 17 is arranged below the nutrient solution drain pipe 6; can avoid external air to enter into the inside of stem cell culture bucket 1 during flowing back through the nutrient solution fluid-discharge tube 6 that is the S type to gas buffering balloon 10 is saved the gas of stem cell culture bucket 1 inside, plays a cushioning effect, takes place the air exchange with the external world when avoiding changing the culture solution, and the inside that leads to stem cell culture bucket 1 receives the pollution, and then influences the preparation of stem cell preparation.
As shown in fig. 1, fig. 2, fig. 4 and fig. 5, a stem cell preparation apparatus includes: a heating water tank 15 which is provided inside the stem cell culture bucket 1, wherein a heating rod 14 is installed inside the heating water tank 15, and the heating water tank 15 is in a ring-shaped structure; the stirring motor 9 is arranged above the culture barrel cover 13, the output end of the stirring motor 9 is provided with a transmission rod 2, the two sides of the transmission rod 2 are fixedly provided with stirring rods 3, the transmission rod 2 penetrates through the interior of the culture barrel cover 13, and the transmission rod 2 is movably connected with the culture barrel cover 13; the water in the heating water tank 15 is heated through the heating rod 14, then the culture solution in the stem cell culture barrel 1 is heated, the culture solution is uniformly heated, the phenomenon that the culture solution is locally overheated and the culture of stem cells is affected is avoided, meanwhile, the culture solution in the stem cell culture barrel 1 is uninterruptedly stirred through the transmission rod 2, the stirring rod 3 and the stirring motor 9, the workload of manual liquid mixing is reduced, and meanwhile, the heating rod 14 and the heating water tank 15 are matched for better heating of the culture solution; the limiting sliding groove 18 is formed in the inner wall of the stem cell culture barrel 1, a limiting sliding block 19 is mounted inside the limiting sliding groove 18, a stem cell containing dish 5 is mounted on one side, away from the limiting sliding groove 18, of the limiting sliding block 19, a filter screen 7 is mounted inside the stem cell containing dish 5, and an operating rod 4 is fixedly mounted above the stem cell containing dish 5; the shape and the size of the stem cell containing dish 5 are matched with the shape and the size of the inside of the stem cell culture barrel 1, and the stem cell containing dish 5 slides in the inside of the stem cell culture barrel 1 through a limiting sliding groove 18 and a limiting sliding block 19; the stem cell tissue can be taken out from the culture solution through the stem cell containing dish 5 and the filter screen 7, so that the separation operation of the stem cell tissue and the culture solution becomes simple and convenient, and the workload of the separation of the stem cell tissue of the staff is reduced.
The working principle is as follows: when the stem cell preparation device is used, firstly, stem cell tissues are placed on a filter screen 7 in a stem cell containing dish 5, then the stem cell containing dish 5 is placed at the bottom in a stem cell culture barrel 1 by operating an operating rod 4, secondly, a culture barrel cover 13 is covered above the stem cell culture barrel 1, then, a liquid injection control valve 12 is opened, a proper amount of culture liquid is injected into the stem cell culture barrel 1 through a liquid injection pipe 11, meanwhile, along with the injection of the culture liquid, gas in the stem cell culture barrel 1 enters the gas buffer balloon 10 through a communicating pipe 8, the liquid injection control valve 12 is closed after the liquid injection is finished, then, a heating rod 14 is electrified to heat water in a heating water tank 15, further, the culture liquid in the stem cell culture barrel 1 is heated, and then, a stirring motor 9 is electrified to work to drive a stirring rod 2 and a stirring rod 3 on a transmission rod 2 to rotate, the culture solution is stirred, when the culture solution is changed at last, the liquid discharge valve 16 is opened at first, the culture solution in the stem cell culture barrel 1 is discharged from the nutrient solution liquid discharge pipe 6 under the action of gravity, the gas in the gas buffer balloon 10 along with the culture solution can enter the stem cell culture barrel 1, the liquid discharge valve 16 is closed after the culture solution in the stem cell culture barrel 1 is discharged, the liquid discharge valve is connected with the liquid injection pipe 11, and the liquid injection control valve 12 is opened to inject the liquid into the stem cell culture barrel 1 so as to complete the liquid change.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A stem cell preparation device, comprising:
the stem cell culture barrel comprises a stem cell culture barrel (1), wherein a culture barrel cover (13) is arranged above the stem cell culture barrel (1), a liquid injection pipe (11) is installed inside the culture barrel cover (13), a liquid injection control valve (12) is installed on the left side of the liquid injection pipe (11), a communicating pipe (8) is fixedly installed on the right side of the liquid injection pipe (11), and a gas buffer balloon (10) is connected above the communicating pipe (8);
and the nutrient solution drain pipe (6) is arranged below the stem cell culture barrel (1), a drain valve (16) is arranged on the left side of the nutrient solution drain pipe (6), and a bolt (17) is arranged below the nutrient solution drain pipe (6).
2. The stem cell preparation device according to claim 1, wherein the liquid injection pipe (11) penetrates through the inside of the culture barrel cover (13), the communication pipe (8) penetrates through the inside of the culture barrel cover (13), and the gas buffering balloon (10) is communicated with the stem cell culture barrel (1) through the communication pipe (8).
3. The stem cell preparation device as claimed in claim 1, wherein the nutrient solution drain pipe (6) is communicated with the stem cell culture barrel (1), and the nutrient solution drain pipe (6) is of an S-shaped structure.
4. The stem cell preparation device according to claim 1, wherein the stem cell culture bucket (1) is further provided with:
the stem cell culture bucket comprises a heating water tank (15) which is arranged inside the stem cell culture bucket (1), wherein a heating rod (14) is arranged inside the heating water tank (15), and the heating water tank (15) is of an annular structure.
5. The stem cell preparation device as claimed in claim 1, wherein the culture drum cover (13) is further provided with:
agitator motor (9), it is installed cultivate the top of bung (13), transfer line (2) are installed to the output of agitator motor (9), the both sides fixed mounting of transfer line (2) has puddler (3), transfer line (2) run through in the inside of cultivateing bung (13), transfer line (2) and cultivation bung (13) swing joint.
6. A stem cell preparation device according to claim 1, wherein said stem cell culture bucket (1) is further provided with:
spacing spout (18), it sets up the inner wall of stem cell culture bucket (1), the internally mounted of spacing spout (18) has spacing slider (19), one side that spacing spout (18) were kept away from in spacing slider (19) is installed the stem cell and is held ware (5), the internally mounted that the stem cell held ware (5) has filter screen (7), the top fixed mounting that the stem cell held ware (5) has action bars (4).
7. The stem cell preparation device according to claim 6, wherein the shape and size of the stem cell containing dish (5) are matched with the shape and size of the inside of the stem cell culture barrel (1), and the stem cell containing dish (5) slides in the inside of the stem cell culture barrel (1) through the limiting sliding chute (18) and the limiting sliding block (19).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123013110.6U CN216550386U (en) | 2021-12-02 | 2021-12-02 | Stem cell preparation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123013110.6U CN216550386U (en) | 2021-12-02 | 2021-12-02 | Stem cell preparation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216550386U true CN216550386U (en) | 2022-05-17 |
Family
ID=81540002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123013110.6U Active CN216550386U (en) | 2021-12-02 | 2021-12-02 | Stem cell preparation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216550386U (en) |
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2021
- 2021-12-02 CN CN202123013110.6U patent/CN216550386U/en active Active
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