CN216366712U - Homogeneity bag that contains filtering capability - Google Patents
Homogeneity bag that contains filtering capability Download PDFInfo
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- CN216366712U CN216366712U CN202122656631.7U CN202122656631U CN216366712U CN 216366712 U CN216366712 U CN 216366712U CN 202122656631 U CN202122656631 U CN 202122656631U CN 216366712 U CN216366712 U CN 216366712U
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- bag
- feed liquid
- bag body
- liquid outlet
- homogenizer
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- 238000001914 filtration Methods 0.000 title claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 48
- 239000000463 material Substances 0.000 claims abstract description 25
- 230000008676 import Effects 0.000 claims 2
- 239000011148 porous material Substances 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 10
- 238000000605 extraction Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 14
- 238000002386 leaching Methods 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 239000012528 membrane Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 238000010828 elution Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 239000000515 collagen sponge Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000010009 beating Methods 0.000 description 4
- 239000003480 eluent Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 108010050327 trypticase-soy broth Proteins 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 238000013019 agitation Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011112 process operation Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of medical instruments, in particular to a homogenizing bag with a filtering function, which comprises a bag body, a material liquid inlet, a filtering net and a material liquid outlet, wherein the material liquid inlet and the material liquid outlet are connected to the bag body and are respectively positioned at two ends of the upper side edge of the bag body, the filtering net is positioned at one end, close to the material liquid outlet, in the bag, and each edge of the filtering net is connected with each edge in the bag body. When the easily decomposed materials are subjected to single or multiple times of extraction operation, the decomposed materials are separated from the extracting solution by only adopting 1 homogeneous bag with a filtering function, no additional filtering program is needed, and a standing mode can be replaced; the whole test is simple and convenient to operate, accurate in result, greatly improves the working efficiency, and saves energy and cost.
Description
Technical Field
The utility model relates to the technical field of medical instruments, in particular to a homogenizing bag with a filtering function.
Background
The information in this background section is only for enhancement of understanding of the general background of the utility model and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
When the microbial limit detection is carried out on a solid or semi-fluid sample, the microbial limit detection is carried out by adopting the modes of bag creeping, ultrasonic elution, stirring (with or without glass beads), vortex mixing, washing, stirring cutting (disintegration), wiping, agar covering, contact plate and the like, a suspension containing the countable microorganisms is obtained by dilution, and the counting is carried out by adopting a plate method and a membrane filtration method. When extraction is performed by a method such as bag creeping, agitation cutting (crushing), vortex mixing, etc., a product to be measured may be decomposed, and the decomposed material may cause difficulty in counting microorganisms. In the prior art, when a microorganism limit experiment is performed on a material which is difficult to count microorganisms due to material decomposition, an extracting solution is obtained by adopting a standing or filtering mode, so that the microorganism limit experiment (membrane filtration or plate pouring) is performed; the above two processing methods still have certain limitations, which are mainly reflected in the following three aspects:
1. when the standing mode is adopted, particulate matters are inevitably doped in the process of transferring the supernatant, and in addition, the standing time may be long, and the deviation of the detection result may be caused;
2. when the filtration mode is adopted, a filtration procedure is additionally added and secondary pollution may be caused to the leaching liquor, so that the detection result is influenced, and the cost is wasted.
3. When the same sample needs to be repeatedly leached, leaching substances can be repeatedly transferred and leached, so that the test difficulty is additionally increased, the test time is prolonged, the test cost is increased, the detection result can be influenced, and the cost is wasted.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problems in the prior art, the utility model aims to provide a homogenizing bag with a filtering function, wherein when the easily decomposed material is subjected to single or multiple leaching operations, the decomposed material is separated from the extracting solution by only adopting 1 homogenizing bag with the filtering function, no additional filtering program is needed, and a standing mode can be replaced; the whole test is simple and convenient to operate, accurate in result, greatly improves the working efficiency, and saves energy and cost.
The technical scheme of the utility model is as follows:
a homogeneous bag with a filtering function comprises a bag body, a feed liquid inlet, a filter screen and a feed liquid outlet, wherein the feed liquid inlet and the feed liquid outlet are connected to the bag body and are respectively positioned at two ends of the upper side edge of the bag body;
preferably, the bag body, the feed liquid inlet and the feed liquid outlet are all made of PP materials;
preferably, the aperture of the filter screen is 75 μm, and the corresponding mesh number is 200 meshes;
preferably, the feed liquid inlet and the feed liquid outlet are both pipe-type outlets, and further preferably, the feed liquid inlet and the feed liquid outlet are both matched with screw caps; the arrangement of the tubular outlet and the screw cap allows for repeated operation of the homogenizer bag of the present invention,
preferably, a writing area is arranged on the outer side of the bag body;
preferably, the side surface of the bag body is provided with an annular handle, so that the homogenizing bag is lifted up during operation;
when the homogenizing bag with the filtering function is used, materials and leaching liquor are added through the material liquid inlet, the leaching liquor is filtered through the filter screen, and an ideal leaching liquor is obtained through the material liquid outlet; the method can be repeatedly operated, does not need to add an additional filtering procedure and a standing procedure, does not cause secondary pollution, and in addition, if needed, the residual decomposed materials can be taken out through a feed liquid inlet, so that the next test is conveniently carried out.
The utility model has the beneficial effects that:
the homogenizing bag with the filtering function solves the problems of deviation of experimental results, low efficiency and energy waste caused by the fact that a filtering program, a standing program and repeated leaching of easily-decomposed materials are added due to the fact that the decomposed materials are difficult to separate from an extracting solution, and the filtering program and the standing program are repeatedly added in the prior art.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the utility model, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the utility model and together with the description serve to explain the utility model and not to limit the utility model.
FIG. 1 is a schematic view of a homogenizer bag with a filtration function according to the present invention;
in the figure, 1, a material liquid inlet, 2, a bag body, 3, a filter screen, 4 and a material liquid outlet.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the utility model as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the utility model. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
As shown in fig. 1, a homogeneous bag with a filtering function comprises a bag body 2, a feed liquid inlet 1, a filter screen 3 and a feed liquid outlet 4, wherein the feed liquid inlet 1 and the feed liquid outlet 4 are connected to the bag body 2 and respectively located at two ends of the upper side of the bag body 2, the filter screen 3 is located at one end of the bag close to the feed liquid outlet 4, and each edge of the filter screen is connected with each edge of the inner part of the bag body;
the feed liquid inlet 1 and the feed liquid outlet 4 are both pipe-type outlets, and the feed liquid inlet 1 and the feed liquid outlet 4 are both matched with screw caps; the arrangement of the tubular outlet and the screw cap allows for repeated operation of the homogenizer bag of the present invention,
further, the bag body, the feed liquid inlet and the feed liquid outlet are all made of PP materials; the aperture of the filter screen is 75 μm, and the corresponding mesh number is 200 meshes; a writing area is arranged on the outer side of the bag body; the side surface of the bag body is provided with an annular handle, so that the homogeneous bag can be lifted up during operation;
example 1
The comparison is made by taking a microorganism limit test of a collagen sponge product as an example. The total amount of the eluent is A, B, wherein the group A is prepared by adopting the homogenizing bag with the filtering function to carry out the preparation of the eluent, and the group B is prepared by adopting the existing disposable sterile homogenizing bag to carry out the preparation of the eluent.
Note that: the AB two groups of experiments are carried out in a clean bench, and the number of the experiments is 1; the homogenizing bag, the collagen sponge product, the required vessel, the reagent solution and the like are all provided aseptically; the process of the comparison test is described as a single sample, and A, B groups need to be subjected to 3 parallel tests in actual operation.
Test procedure of group A:
the method comprises the following steps: adding 50mg of collagen sponge product into a homogenizing bag through a material liquid inlet, continuously adding 100 mu L of bacillus subtilis suspension (the number of colonies contained in each 0.1mL is less than or equal to 100CFU) into the sample through the material liquid inlet, drying in a laminar flow environment, adding 50mL of sodium chloride leaching liquor (0.9%, m/v), and placing on a homogenizer for beating and extracting for 1 min. The sample is now in a dispersed state.
Step two: passing the eluate through a filter screen, directly transferring to a membrane filter via a feed liquid outlet, filtering, collecting bacteria in the eluate on a filter membrane, carefully taking out the filter membrane, and sticking the bacteria face upwards on a trypticase soy peptone agar plate; the first elution was completed.
Step three: continuously adding 50mL of sodium chloride leaching liquor (0.9%, m/v) through the feed liquid inlet, placing on a homogenizer for beating and extracting for 1min, and repeating the operation of the second step; a second eluted filter was obtained.
Step four: and repeating the operation of the third step for 3 times to obtain filter membranes for elution for the third time, the fourth time and the fifth time respectively.
Step five: the residue after five elutions was taken out through the feed liquid outlet, placed in a sterile plate, and covered with melted tryptone soy agar.
Step six: placing the prepared culture dish in a constant temperature incubator upside down, and culturing for 3 days at the temperature of 30-35 ℃; the results were observed and recorded.
The experimental results are as follows:
test procedure of group B:
the method comprises the following steps: adding 50mg collagen sponge product into a homogenizing bag, continuously adding 100 μ L Bacillus subtilis suspension (the colony count contained in each 0.1mL is less than or equal to 100CFU) into the collagen sponge product, drying in laminar flow environment, adding 50mL sodium chloride leaching solution (0.9%, m/v), and beating and extracting for 1min on a homogenizer; the sample is now in a dispersed state.
Step two: putting the leaching solution into a membrane filter through a disposable sterile filter screen (200 meshes), carrying out suction filtration, collecting bacteria in the eluent on the filter membrane, carefully taking out the filter membrane, and sticking the bacteria face up to a trypticase soy peptone agar plate; the first elution was completed.
Step three: transferring the residue on the disposable sterile filter screen to the original homogenizing bag, adding 50mL of sodium chloride leaching solution (0.9%, m/v), and beating and extracting for 1 min. Repeating the operation of the second step; a second eluted filter was obtained.
Step four: and repeating the operation of the third step for 3 times to obtain filter membranes for elution for the third time, the fourth time and the fifth time respectively.
Step five: the residue after five elutions was taken out through the feed liquid outlet, placed in a sterile plate, and covered with melted tryptone soy agar.
Step six: the prepared culture dish is placed in a constant temperature incubator upside down and cultured for 3 days at the temperature of 30-35 ℃. The results were observed and recorded. The experimental results are as follows:
the test result shows that: compared with the prior art, the product is adopted for pretreatment, the whole process is simple to operate, the result recovery rate is improved by 10%, the accuracy is relatively improved by 10%, the operation time of a single experiment is shortened by half, when the detection method is confirmed, the method requirement can be met by eluting for 3 times, and the whole experiment time is shortened again by adopting an independent filter for filtering and eluting for 4 times. The data result shows that the novel product has the characteristics of simple and convenient operation, reduced risk of process operation pollution, improved accuracy, partial working procedures, improved working efficiency and the like, and saves energy and cost as much as possible.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The utility model provides a homogeneity bag that contains filtering capability which characterized in that, homogeneity bag contains the bag body, feed liquid import, filter screen and feed liquid export, feed liquid import and feed liquid export all are connected on the bag body, are located the both ends of bag body top side respectively, and the filter screen is located the bag in the one end that is close to the feed liquid export, and each edge of filter screen is connected with each edge of the internal portion of bag.
2. A homogenizer bag with a filtration function as claimed in claim 1, wherein the bag body, the feed liquid inlet and the feed liquid outlet are made of PP material.
3. The homogenizer bag with a filtration function as claimed in claim 1, wherein the filter mesh has a pore size of 75 μm and a corresponding mesh size of 200 mesh.
4. A homogenizer bag with a filtration function as claimed in claim 1, wherein the feed liquid inlet and the feed liquid outlet are both tube outlets.
5. The homogenizer bag with a filtration function as claimed in claim 1, wherein the feed liquid inlet and the feed liquid outlet are both provided with screw caps.
6. The homogenizer bag with a filtration function as claimed in claim 1, wherein a writing area is provided on the outer side of the bag body.
7. The homogenizer bag with a filtration function as claimed in claim 1, wherein a ring-shaped handle is provided on a side surface of the bag body to lift the homogenizer bag in operation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122656631.7U CN216366712U (en) | 2021-10-29 | 2021-10-29 | Homogeneity bag that contains filtering capability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122656631.7U CN216366712U (en) | 2021-10-29 | 2021-10-29 | Homogeneity bag that contains filtering capability |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216366712U true CN216366712U (en) | 2022-04-26 |
Family
ID=81248989
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122656631.7U Active CN216366712U (en) | 2021-10-29 | 2021-10-29 | Homogeneity bag that contains filtering capability |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216366712U (en) |
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2021
- 2021-10-29 CN CN202122656631.7U patent/CN216366712U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder |
Address after: No. 7 Gao'an Street, Economic and Technological Development Zone, Yantai City, Shandong Province, 265599 Patentee after: SHANDONG JUNXIU BIOTECHNOLOGY CO.,LTD. Address before: 264006 overseas Chinese entrepreneurship Park, No.32 Zhujiang Road, Yantai Economic and Technological Development Zone, Shandong Province Patentee before: SHANDONG JUNXIU BIOTECHNOLOGY CO.,LTD. |
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| CP02 | Change in the address of a patent holder |