CN210458189U

CN210458189U - Device for rapidly detecting food-borne pathogenic microorganisms in fresh-cut fruits and vegetables

Info

Publication number: CN210458189U
Application number: CN201920150746.9U
Authority: CN
Inventors: 王记莲
Original assignee: Yancheng Institute of Industry Technology
Current assignee: Yancheng Institute of Industry Technology; Yancheng Vocational Institute of Industry Technology
Priority date: 2019-01-28
Filing date: 2019-01-28
Publication date: 2020-05-05
Anticipated expiration: 2029-01-28

Abstract

The utility model discloses a be arranged in fresh-cut fruit vegetables food source venereal microorganism short-term test device, including sample liquid cavity, pump machine and enrichment room, filter membrane board and culture substrate, the liquid outlet and the pump machine one end of sample liquid cavity be connected, the other end of pump machine one end is connected with the upside of enrichment room, the lower extreme of enrichment room be connected with the waste liquid pipe and be connected with waste liquid collecting container, the utility model has the advantages of reasonable design, be convenient for improve fresh-cut fruit vegetables sample liquid measure and to cultivate wherein microorganism closure enrichment, effectively improve detection efficiency and effect, easy operation adopts the fungus class color development culture medium to cultivate the microorganism of collection simultaneously, has effectively improved the efficiency of testing result.

Description

Device for rapidly detecting food-borne pathogenic microorganisms in fresh-cut fruits and vegetables

Technical Field

The utility model relates to a quick detection device of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables.

Background

The fresh-cut fruits and vegetables not only keep the original fresh state of the fruits and vegetables, but also are processed to ensure that the products are clean and sanitary, and belong to the category of clean vegetables. The microbial compound detection reagent is natural, nutritional, fresh, convenient and high in availability, meets the requirements of people on life styles of pursuing fast pace and health and the like, and has the advantages of various pathogenic microorganisms, rapid variation and continuous development and advancing of an inspection technology for rapidly identifying the pathogenic microorganisms. At present, the pathogenic microorganism detection methods widely applied mainly include direct smear microscopy, separation culture, biochemical reaction, serological reaction, nucleic acid molecule hybridization, gene chip, polymerase chain reaction and the like, and the chromogenic medium is a microorganism culture medium for selective growth and identification of microorganisms. In the last two decades, it has become more and more widely used and even replaced some of the original common culture media in many aspects. For example, the foreign proved that the vibrio chromogenic culture medium can replace TCBS to culture vibrio parahaemolyticus, and the identification accuracy is obviously higher than that of TCBS. In addition, the chromogenic medium integrates culture and identification, so that the time of workers is saved, and the identification accuracy is improved.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the above-mentioned not enough that exists among the prior art, and provide a rational in infrastructure design's a be arranged in fresh-cut fruit vegetables food source pathogenic microorganism short-term test device.

The utility model provides a technical scheme that above-mentioned problem adopted is: the utility model provides a be arranged in fresh-cut fruit vegetables food source venereal microorganism short-term test device, includes appearance liquid cavity, pump machine and enrichment room, filter membrane board and culture substrate, the liquid outlet and the pump machine one end of appearance liquid cavity be connected, the other end of pump machine one end is connected with the upside of enrichment room, the lower extreme of enrichment room be connected with the waste liquid pipe and be connected with waste liquid collecting container, the filter membrane board arrange and be provided with a plurality of millipore filtration membrane that are used for cutting the microorganism and pass through, filter membrane board level fix in the cavity of enrichment room, the filter membrane board divide into first cavity and second cavity with the enrichment room, the connection port setting of pump machine and enrichment room on first cavity, waste liquid pipe and enrichment room connection port set up on the second cavity, enrichment room upside be provided with and be used for the sealed lid that the filter membrane board took off, culture substrate be provided with a plurality ofly with millipore filtration membrane position, The culture medium that size and quantity are corresponding, the border of culture substrate and filter membrane board be provided with respectively and be used for its snap-fit of reciprocal anchorage for culture medium and the laminating of the porous filter membrane enrichment microorganism that corresponds.

Further: the liquid outlet of sample liquid cavity be provided with the coarse filtration filter core for filter the coarse particle that probably exists in the sample liquid.

Further: the culture mediums of the culture substrate are a plurality of genus chromogenic culture mediums for detecting corresponding pathogenic microorganisms.

Further: the culture medium adopts a porous structure.

Further: the plug connector constitute by grafting grooving and grafting net piece, grafting grooving and grafting net piece mutually support, the grafting grooving set up at the filter membrane board terminal surface, the grafting net setting at the culture substrate terminal surface, the grafting net insert through interference fit and establish in the grafting grooving for culture medium and the laminating of the millipore filtration membrane enrichment microorganism one side that corresponds.

Compared with the prior art, the utility model, have following advantage and effect: structural design is reasonable, is convenient for improve fresh-cut fruit vegetables appearance liquid measure and cultivates the microorganism interception enrichment wherein, effectively improves detection efficiency and effect, and easy operation adopts the fungus genus color development culture medium to cultivate the microorganism of gathering simultaneously, has effectively improved the efficiency of testing result.

Drawings

FIG. 1 is a schematic structural diagram of a device for rapidly detecting food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to an embodiment of the present invention.

FIG. 2 is a schematic view of the connection structure of the filter plate and the culture substrate according to the embodiment of the present invention.

Detailed Description

The present invention will be described in further detail by way of examples with reference to the accompanying drawings, which are illustrative of the present invention and are not intended to limit the present invention.

Referring to fig. 1-2, the device for rapidly detecting food-borne pathogenic microorganisms in fresh-cut fruits and vegetables in this embodiment includes a sample liquid chamber 1, a pump 2, an enrichment chamber 3, a filter membrane plate 4 and a culture substrate 5, wherein a liquid outlet of the sample liquid chamber 1 is connected with one end of the pump 2, the other end of one end of the pump 2 is connected with the upper side of the enrichment chamber 3, a waste liquid pipe 61 is connected with the lower end of the enrichment chamber 3 and is connected with a waste liquid collecting container 6, the filter membrane plate 4 is provided with a plurality of microporous filter membranes 41 for cutting off the passage of microorganisms, the filter membrane plate 4 is horizontally fixed in the cavity of the enrichment chamber 3, the enrichment chamber 3 is divided into a first cavity 31 and a second cavity 32 by the filter membrane plate 4, a connection port of the pump 2 and the enrichment chamber 3 is arranged on the first cavity 31, a connection port of the waste liquid pipe 61 and the enrichment chamber 3 is arranged on the second cavity 32, enrichment room 3 upside be provided with be used for the sealed lid 33 that filter membrane board 4 taken off, culture substrate 5 be provided with a plurality of and the corresponding culture medium 51 of millipore filtration 41 position, size and quantity, culture substrate 5 and filter membrane board 4 border be provided with the buckle spare that is used for its reciprocal anchorage respectively for culture medium 51 laminates with the corresponding millipore filtration 41 enrichment microorganism one side, the liquid outlet of sample liquid cavity 1 be provided with coarse filter core 11, a plurality of culture medium 51 of culture substrate 5 be a plurality of the genus of being used for detecting corresponding pathogenic microorganism and show color medium 51, culture medium 51 adopt porous structure, the plug connector constitute by grafting grooving 42 and grafting net piece 52, grafting grooving 42 and grafting net piece 52 mutually support, grafting grooving 42 set up at filter membrane board 4 terminal surfaces, the grafting net setting at culture substrate 5 terminal surfaces, the plugging grids are plugged in the plugging mesh grooves 42 in an interference fit manner, so that the culture medium 51 is attached to the microorganism-enriched surface of the corresponding microporous filter membrane 41.

The quick detection device for the food-borne pathogenic microorganisms in the fresh-cut fruits and vegetables related to the embodiment adopts the following steps: fresh-cut fruit and vegetable filtrate or buffer cleaning sample liquid thereof is placed in the sample liquid chamber 1, the filter membrane plate 4 is arranged in the enrichment chamber 3 at the moment, the edge of the filter membrane plate 4 can be fixed on a fixed groove of the filter membrane plate 4 through interference fit or fixed in the enrichment chamber 3 through a bolt piece, at the moment, the pump body works, the fresh-cut fruit and vegetable filtrate or buffer cleaning sample liquid thereof is pumped into the enrichment chamber 3 for filtration, microorganisms are cut off by the microporous filter membrane 41 of the filter membrane plate 4, after the operation is finished, the filter membrane plate 4 is taken out and is connected and fixed with the culture substrate 5, so that the culture medium 51 is attached to the side of the microporous filter membrane 41 with the enriched microorganisms, and is placed in the culture chamber for microorganism culture operation, because a plurality of culture mediums 51 of the culture medium 51 of a plurality of genera for detecting the corresponding pathogenic microorganisms, metabolites of the microorganisms in the enrichment process develop color on the culture medium 51, therefore, it can be known from the color development of the corresponding genus chromogenic medium 51 that it contains the corresponding genus microorganism, and the detection efficiency is high, for example: if O157 in the common sample liquid: rapid detection of H7 strain, O157: h7 bacteria are purple, and colibacillus and coliform bacteria are dark blue; other bacterium appear yellow or colorless, and listeria monocytogenes is blue, has an opaque ring around the bacterial colony, and the like, in this embodiment, listeria monocytogenes blue, have an opaque ring around the bacterial colony, for example as shown in fig. 2, connection structure is stable not only, the location of being convenient for, culture medium 51 liquid that corresponds simultaneously through its structure mutual isolation, the utility model has the advantages of reasonable design, be convenient for improve fresh-cut fruit vegetables appearance liquid measure and to wherein microorganism interception enrichment culture, effectively improve detection efficiency and effect, and easy operation adopts the fungus genus to develop culture medium 51 to the microorganism of gathering simultaneously, has effectively improved the efficiency of testing result.

The above description in this specification is merely illustrative of the present invention. Various modifications, additions and substitutions may be made by those skilled in the art without departing from the scope of the invention as defined in the accompanying claims.

Claims

1. A quick detection device for food-borne pathogenic microorganisms in fresh-cut fruits and vegetables is characterized in that: comprises a sample liquid chamber, a pump, an enrichment chamber, a filter membrane plate and a culture substrate, wherein a liquid outlet of the sample liquid chamber is connected with one end of the pump, the other end of one end of the pump is connected with the upper side of the enrichment chamber, the lower end of the enrichment chamber is connected with a waste liquid pipe and a waste liquid collecting container, the filter membrane plate is arranged and provided with a plurality of microporous filter membranes for cutting microorganisms to pass through, the filter membrane plate is horizontally fixed in a cavity of the enrichment chamber and is divided into a first cavity and a second cavity, a connecting port of the pump and the enrichment chamber is arranged on the first cavity, a connecting port of the waste liquid pipe and the enrichment chamber is arranged on the second cavity, a sealing cover used for taking the filter membrane plate is arranged on the upper side of the enrichment chamber, and the culture substrate is provided with a plurality of culture media corresponding to the positions, sizes and quantities of the microporous filter membranes, the culture substrate and the filter membrane plate are provided with interference fit plug connectors which are mutually attached and fixed, so that the culture substrate is attached to one side of the corresponding microporous filter membrane enriched microorganism.

2. The device for rapidly detecting food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 1, is characterized in that: the liquid outlet of sample liquid cavity be provided with the colating filter core.

3. The device for rapidly detecting food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 2, is characterized in that: the culture mediums of the culture substrate are a plurality of genus chromogenic culture mediums for detecting corresponding pathogenic microorganisms.

4. The device for rapidly detecting food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 1, is characterized in that: the culture medium adopts a porous structure.

5. The device for rapidly detecting food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 1, is characterized in that: the plug connector constitute by grafting grooving and grafting net piece, grafting grooving and grafting net piece mutually support, the grafting grooving set up at the filter membrane board terminal surface, the grafting net setting at the culture substrate terminal surface, the grafting net insert through interference fit and establish in the grafting grooving for culture medium and the laminating of the millipore filtration membrane enrichment microorganism one side that corresponds.