CN105132275A - Microorganism gathering device and application thereof - Google Patents
Microorganism gathering device and application thereof Download PDFInfo
- Publication number
- CN105132275A CN105132275A CN201510625884.4A CN201510625884A CN105132275A CN 105132275 A CN105132275 A CN 105132275A CN 201510625884 A CN201510625884 A CN 201510625884A CN 105132275 A CN105132275 A CN 105132275A
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- CN
- China
- Prior art keywords
- filter membrane
- enrichment
- microorganism
- pump
- aeration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
- C12M29/08—Air lift
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Abstract
The invention provides a microorganism gathering device. The microorganism gathering device consists of an aeration pump, a seal cover, an aeration pipeline, two filter membranes, an aeration device, an outer wall, a flow-through cover, a water discharging pipeline and a water pump, wherein a culture chamber is formed between the two filter membranes, the aeration pipeline is arranged in the culture chamber and connected to the aeration pump, and a space is formed below the seal cover and connected to the water pump. The microorganism gathering device is reasonable in design, low in production cost and simple, the upper layer and lower layer of porous films of the device are used for filtering selectively gathered bacteria, actinomycetes or fungus, and the microorganism gathering efficiency can be improved by virtue of a suction force of the external water pump. Moreover, in a natural environment, a screening culture medium is added into the internal culture chamber to carry out the screening and proliferation. The microorganism gathering device has advantages of short gathering time, high gathering efficiency, high screening specificity and the like. The invention aims at providing an application of the microorganism gathering device in gathering water microorganisms.
Description
Technical field
The invention belongs to biological technical field, relate to the equipment of specified microorganisms and the application of this equipment in a kind of enrichment water body.
Background technology
Microbe species is various, only generally estimates more than 1,000,000 kinds in ocean, and up-to-date marine microorganism gene order-checking research proves the understanding that its population wants superman's class far away previous.However, current educable microorganism is still limited in only a few (about 1%) monoid, and nature comprises in ocean and has 99% microorganism traditional experiment room method to cultivate, and is the old problem of microbiology over more than 70 year.These uncultured microorganisms are the important keys finding and enrich new resources.
Summary of the invention
The object of this invention is to provide a kind of Enrichment by Microorganisms acquisition means, be made up of aeration pump, closing cap, aerated conduit, the first filter membrane, aerator, outer wall, the second filter membrane, negotiable lid, drainage pipeline, lift pump, first filter membrane, the second filter membrane are arranged on inwall, filter sizes is 0.03mm-0.5mm, between form culturing room, aerator is had in culturing room, outside aeration pump is connected to by aerated conduit, space is formed between first filter membrane and closing cap 2, drainage pipeline to be arranged on closing cap and to communicate with space, and lift pump connects drainage pipeline.Device selects impregnating material, as Stainless steel 316 L, and carbon steel, 92 bronze medals etc.
Another object of the present invention is to provide the application of described Enrichment by Microorganisms acquisition means in enrichment microorganisms in water.Realized by following steps: corresponding screening culture medium is configured rear injection culturing room by the microbe species difference according to enrichment, then being positioned over by device thinks in the water body of enrichment, start aeration pump and lift pump, because the membrane pore size of the first filter membrane, the second filter membrane is different, the microorganism of filtering different size can be retained.Through work after a while, withdrawing device, opens negotiable lid, can release the water after process, has preliminary effect of filtering large object.A lot of specific microorganism is just enriched in first filter membrane, the second filter membrane and culturing room.
Apparatus of the present invention are reasonable in design, and cost of manufacture is low and simple.By this explanation device, utilize upper and lower two-layer microporous membrane filtration selective enrichment bacterium, actinomycetes or fungi, improve microorganism bioaccumulation efficiency by the suction of outside lift pump simultaneously.And under physical environment, inner culturing room adds screening culture medium and carries out screening propagation.The advantages such as it is short that the present invention has enrichment time, and bioaccumulation efficiency is high, and screening specificity is strong.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is embodiment 2COD variation diagram.
Fig. 3 is embodiment 3 ammonia nitrogen variation diagram.
Embodiment
The present invention is further described in conjunction with the accompanying drawings and embodiments.
Embodiment 1:
See Fig. 1, the present invention is made up of aeration pump 1, closing cap 2, aerated conduit 3, first filter membrane 4, aerator 5, outer wall 6, second filter membrane 7, negotiable lid 8, drainage pipeline 9, lift pump 10, first filter membrane 4, second filter membrane 7 is arranged on inwall, filter sizes is 0.03mm-0.5mm, between form culturing room 12, aerator 5 is had in culturing room 12, outside aeration pump 1 is connected to by aerated conduit 3, space 11 is formed between first filter membrane 4 and closing cap 2, drainage pipeline 9 to be arranged on closing cap 2 and to communicate with space 11, and lift pump 10 connects drainage pipeline 9.
During use, corresponding screening culture medium is configured rear injection culturing room 12 by the microbe species difference according to enrichment, then being positioned over by device thinks in the water body of enrichment, start aeration pump 1 and lift pump 10, because the membrane pore size of the first filter membrane 4, second filter membrane 7 is different, the microorganism of filtering different size can be retained.Through work after a while, withdrawing device, opens negotiable lid 8, can release the water after process, has preliminary effect of filtering large object.Just be enriched a lot of specific microorganism in first filter membrane 4, second filter membrane 7 and culturing room 12, collected by ordinary method.
Embodiment 2
Certain paper mill COD of sewage is very high, 10325mg/L, biochemical treatment difficulty, needs enrichment to degrade the bacterial classification of this pollutant.During use, by 1 liter of screening culture medium: ammonium sulfate 5g/L, peptone 1g/L, potassiumphosphate 1g/L, configure rear injection culturing room 12, being positioned over by device thinks in the water body of enrichment, be positioned in equipment by the first filter membrane 4, second filter membrane 7, the first filter membrane 4 aperture is 0.2mm, allows the nutritive substance in environmental sample thing and actinomycetes to enter culturing room to catch more bacterium and actinomycetes, second filter membrane 7 aperture is 0.4mm, and the yeast in restriction environmental sample thing and fungi enter.Start aeration pump 1 and lift pump 10, through the non-stop run of 24 hours, open equipment, the first filter membrane 4, second filter membrane 7 is taken off, wash-out in screening culture medium, a large amount of sewage degradation bacteria of liquid cylinder accumulation in elutriant and culturing room 12.Again after screening and culturing, obtain a degradation flora ZJ, aeration 24 hours, COD degradation rate more than 80% (Fig. 2).
Embodiment 3
Certain seawater aquaculture plant effuent ammonia nitrogen is very high, 10mg/L, COD is not very high, it is not fine for carrying out degradation of ammonia nitrogen effect for directly utilizing market bacterial classification, mainly because the salinity in seawater is higher, with the culture environment of bacterial strain, culture environment of aquatic products requires that difference causes effect to have a greatly reduced quality simultaneously.Therefore apparatus of the present invention are utilized to carry out the enrichment of original position bacterial classification.During use, by 1 liter of screening culture medium: peptone 100mg/L, potassiumphosphate 10mg/L, configure rear injection culturing room 12, being positioned over by device thinks in the water body of enrichment, first filter membrane 4, second filter membrane 7 is positioned in equipment, first filter membrane 4 aperture is 0.03mm, the nutritive substance in environmental sample thing and actinomycetes are allowed to enter culturing room to catch more bacterium and actinomycetes, simultaneously the microorganism such as restricting bacterial is extracted out by lift pump, second filter membrane 7 aperture is 0.4mm, and the yeast in restriction environmental sample thing and fungi enter.Start aeration pump 1 and lift pump 10, through the non-stop run of 24 hours, open equipment, the first filter membrane 4, second filter membrane 7 is taken off, wash-out in screening culture medium, a large amount of sewage degradation bacteria of liquid cylinder accumulation in elutriant and culturing room 12.Again after screening and culturing, obtain a denitrifying bacteria TD and denitrogenation fungi ZD, 24 hours, ammonia nitrogen degradation rate more than 60% (Fig. 3).
Claims (4)
1. an Enrichment by Microorganisms acquisition means, it is characterized in that, by aeration pump (1), closing cap (2), aerated conduit (3), first filter membrane (4), aerator (5), outer wall (6), second filter membrane (7), negotiable lid (8), drainage pipeline (9), lift pump (10) forms, first filter membrane (4), second filter membrane (7) is arranged on inwall, filter sizes is 0.03mm-0.5mm, between form culturing room (12), aerator (5) is had in culturing room (12), outside aeration pump (1) is connected to by aerated conduit (3), space (11) is formed between first filter membrane (4) and closing cap (2), drainage pipeline (9) is arranged on closing cap (2) and goes up and communicate with space (11), lift pump (10) connects drainage pipeline (9).
2. a kind of Enrichment by Microorganisms acquisition means according to claim 1, is characterized in that, device adopts impregnating material, selects Stainless steel 316 L, carbon steel or 92 bronze medals.
3. the application of a kind of Enrichment by Microorganisms acquisition means according to claim 1 and 2 in enrichment microorganisms in water.
4. application according to claim 3, it is characterized in that, realized by following steps: corresponding screening culture medium is configured rear injection culturing room (12) by the microbe species difference according to enrichment, then being positioned over by whole device thinks in the water body of enrichment, start aeration pump (1) and lift pump (10), due to the first filter membrane 4, the membrane pore size of the second filter membrane 7 is different, the microorganism of filtering different size can be retained, through work after a while, withdrawing device, open negotiable lid (8), release the water after process, first filter membrane (4), specific microorganism is just enriched with in second filter membrane (7) and culturing room (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510625884.4A CN105132275A (en) | 2015-09-28 | 2015-09-28 | Microorganism gathering device and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510625884.4A CN105132275A (en) | 2015-09-28 | 2015-09-28 | Microorganism gathering device and application thereof |
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| Publication Number | Publication Date |
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| CN105132275A true CN105132275A (en) | 2015-12-09 |
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| CN201510625884.4A Pending CN105132275A (en) | 2015-09-28 | 2015-09-28 | Microorganism gathering device and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111172022A (en) * | 2020-02-21 | 2020-05-19 | 广西贵港华堂天诺微生物科技有限公司 | In-situ microorganism collection system |
| CN112143617A (en) * | 2020-09-11 | 2020-12-29 | 铜仁职业技术学院 | Target bacterium screening device, preparation method and application method thereof |
Citations (4)
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|---|---|---|---|---|
| US20070298451A1 (en) * | 2006-06-27 | 2007-12-27 | Sebastien Ribault | Method and unit for preparing a sample for the microbiological analysis of a liquid |
| CN101434902A (en) * | 2008-12-18 | 2009-05-20 | 崔生发 | Open type cyclic tubular microbial dialysis cultivation system |
| CN103298922A (en) * | 2011-03-29 | 2013-09-11 | 南京新诺丹生物技术有限公司 | Multifunctional bioreactor system and methods for cell sorting and culturing |
| CN204999899U (en) * | 2015-09-28 | 2016-01-27 | 浙江大学舟山海洋研究中心 | Little biological concentration device |
-
2015
- 2015-09-28 CN CN201510625884.4A patent/CN105132275A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070298451A1 (en) * | 2006-06-27 | 2007-12-27 | Sebastien Ribault | Method and unit for preparing a sample for the microbiological analysis of a liquid |
| CN101434902A (en) * | 2008-12-18 | 2009-05-20 | 崔生发 | Open type cyclic tubular microbial dialysis cultivation system |
| CN103298922A (en) * | 2011-03-29 | 2013-09-11 | 南京新诺丹生物技术有限公司 | Multifunctional bioreactor system and methods for cell sorting and culturing |
| CN204999899U (en) * | 2015-09-28 | 2016-01-27 | 浙江大学舟山海洋研究中心 | Little biological concentration device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111172022A (en) * | 2020-02-21 | 2020-05-19 | 广西贵港华堂天诺微生物科技有限公司 | In-situ microorganism collection system |
| CN112143617A (en) * | 2020-09-11 | 2020-12-29 | 铜仁职业技术学院 | Target bacterium screening device, preparation method and application method thereof |
| CN112143617B (en) * | 2020-09-11 | 2023-10-31 | 铜仁职业技术学院 | Target bacterium screening device, preparation method and use method thereof |
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Application publication date: 20151209 |
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