CN216337700U - Magnetic attraction device and gene detection equipment - Google Patents

Abstract

The embodiment of the application provides a magnetic attraction device and gene detection equipment, and belongs to the technical field of gene detection. The magnetic attraction device is matched with the gene detection kit and comprises a rotating shaft, a bottom attraction component and a driving piece, wherein the rotating shaft is matched with a piston cylinder in the gene detection kit to drive the piston cylinder to rotate, and a first magnetic attraction channel is arranged in the rotating shaft along the axial direction of the rotating shaft; the bottom suction assembly is used for carrying out magnetic attraction positioning on magnetic beads in the piston cavity of the piston cylinder, is movably arranged in the first magnetic attraction channel and has a first position and a second position; when the bottom suction assembly is at the first position, the bottom suction assembly can be attached to the bottom of the piston cavity; when the bottom suction assembly is at the second position, the bottom suction assembly is separated from the bottom of the piston cavity; the driving piece is used for driving the bottom suction assembly to be switched between the first position and the second position. The magnetic attraction device occupies small space and can improve the compactness of gene detection equipment.

Description

Magnetic attraction device and gene detection equipment

Technical Field

The application relates to the technical field of gene detection, in particular to a magnetic attraction device and gene detection equipment.

Background

Gene detection has been widely used in the fields of biomedicine and the like, and the whole process of gene detection generally comprises nucleic acid extraction, PCR amplification and detection. Nucleic acid extraction is also a very important part of the gene detection process. In the process of nucleic acid extraction, the magnetic bead method has the advantages of high extraction purity, low technical requirement, short extraction time, automation realization and the like, and is widely applied to nucleic acid extraction.

When using, the existing magnetic bead method generally realizes magnetic attraction to magnetic beads in a reagent kit through a lifting magnetic frame, the existing lifting magnetic frame can often use a special motor to drive the magnetic frame to ascend or descend so as to realize the attaching and separating functions with the reagent kit, the whole magnetic frame is large in size, the relevance between the lifting magnetic frame and gene detection equipment is poor, the lifting magnetic frame occupies a large space of the gene detection equipment, and the overall compactness of the gene detection equipment is influenced.

SUMMERY OF THE UTILITY MODEL

The embodiment of the application provides a magnetism device and gene detection equipment, can improve the compactness of magnetism device in gene detection equipment of inhaling.

In a first aspect, an embodiment of the present application provides a magnetic attraction device, where the magnetic attraction device is used for being matched with a gene detection kit, the magnetic attraction device includes a rotating shaft, a bottom attraction assembly and a driving member, the rotating shaft is used for being matched with a piston cylinder in the gene detection kit to drive the piston cylinder to rotate, and a first magnetic attraction channel is arranged in the rotating shaft along an axial direction of the rotating shaft; the bottom suction assembly is used for carrying out magnetic attraction positioning on magnetic beads in the piston cavity of the piston cylinder, is movably arranged in the first magnetic attraction channel and has a first position and a second position; when the bottom suction assembly is at the first position, the bottom suction assembly can be attached to the bottom of the piston cavity so as to perform magnetic attraction positioning on the magnetic beads in the piston cavity; when the bottom suction assembly is at the second position, the bottom suction assembly is separated from the bottom of the piston cavity; the driving piece is used for driving the bottom suction assembly to be switched between the first position and the second position.

Among the above-mentioned technical scheme, the bottom of the piston cylinder in the gene detection kit needs to cooperate with the pivot, thereby make the piston cylinder rotate, thereby realize the reagent exchange of gene detection kit, consequently make arranging of end-sucking subassembly comparatively difficult, and in this scheme through with end-sucking subassembly integration in the pivot, make it realize the magnetic bead in the minimum space in the pivot and adsorb the function, make gene detection equipment's compact structure better, more do benefit to the space layout of magnetic attraction device in gene detection equipment, improve gene detection equipment's whole compactness. Under the effect of driving piece, the end is inhaled the subassembly and can be removed in the passageway is inhaled to first magnetism in the pivot, and the end is inhaled the subassembly and can be removed to the bottom in piston chamber, inhales the location to the magnetic bead in the piston chamber, and the magnetic bead of being convenient for stops in the piston cylinder to with the reagent liquid separation in the piston cylinder, let reagent liquid return to in the reagent chamber that corresponds.

In some embodiments, when the bottom suction assembly is in the first position, the bottom suction assembly at least partially protrudes out of the first magnetic attraction channel; when the bottom suction assembly is at the second position, the bottom suction assembly retracts into the first magnetic suction channel.

Among the above-mentioned technical scheme, the subassembly is inhaled at the end can stretch out outside first magnetism is inhaled the passageway, and the subassembly is inhaled at the end like this and can be inhaled the piston chamber that is arranged in the outer gene detect kit of first magnetism and inhale the passageway and inhale magnetism, even piston barrel bottom can not contact with the pivot, the subassembly is inhaled at the same end and also can stretch out outside first magnetism is inhaled the passageway, has improved the end and has inhaled the stroke of subassembly in first magnetism is inhaled the passageway, and the range of application is wider.

In some embodiments, the bottom suction assembly includes a first magnetic element, a first connecting seat, a second connecting seat, and an elastic member, the first magnetic element is used for magnetically positioning magnetic beads in the piston cavity; the first connecting seat is used for mounting the first magnetic element; the second connecting seat is used for being connected with the driving piece; the elastic piece is connected between the first connecting seat and the second connecting seat.

Among the above-mentioned technical scheme, be equipped with the elastic component between first connecting seat and the second connecting seat, when the driving piece drove bar magnet upward movement, the elastic component is owing to have buffering and deformation effect, and the elastic component can avoid leading to the problem of the overstroke of first magnetism suction element or owe the stroke because of manufacturing, installation error to the elastic force of elastic component can make the end inhale the subassembly and hug closely with the bottom in piston chamber, has improved the magnetism effect of inhaling of end inhale the subassembly.

In some embodiments, a sliding hole is formed in the first connecting seat, one end of the first connecting seat is connected to the first magnetic attraction element, the other end of the first connecting seat is slidably sleeved on the second connecting seat through the sliding hole, and the elastic element is disposed in the sliding hole.

Among the above-mentioned technical scheme, through set up the slide opening in first connecting seat, the slide opening can lead the slip between first connecting seat and the second connecting seat to also restrict the flexible direction of elastic component, ensured the stability of first magnetism and inhale the component removal process.

In some embodiments, the bottom suction assembly further includes a guide rod extending along an axial direction of the rotating shaft, the first connecting seat is slidably sleeved on the guide rod, one end of the guide rod is connected to the second connecting seat, the other end of the guide rod is provided with a limiting portion for limiting the first connecting seat to be separated from the second connecting seat, and the elastic member is sleeved on the guide rod.

Among the above-mentioned technical scheme, the guide bar can play the effect of connecting first connecting seat and second connecting seat to wear to establish the guide effect for the elastic component provides, spacing portion can restrict first connecting seat and break away from the second connecting seat, improves the stability of inhaling the subassembly when removing at the bottom.

In some embodiments, the first magnetic-attracting element is magnetically coupled with the first coupling seat.

Among the above-mentioned technical scheme, it is tight to inhale through magnetic force between the component is inhaled to first magnetism and the first connecting seat the two, not only can realize that first magnetism inhales the component and is connected with first connecting seat, and first magnetism inhales can there be relative rotation between component and the first connecting seat, and can not have relative motion from top to bottom, and when the pivot drove the piston chamber and rotates and switch the runner, first magnetism inhale the component can not and the pivot between there be relative rotation, can follow the pivot and rotate.

In some embodiments, the first magnetic attraction element is an annular structure for attracting magnetic beads in the piston cavity when the bottom attraction assembly is in the first position; the first magnetic attraction element is provided with a notch along the axial direction in a penetrating way, and the inner wall of the first magnetic attraction channel is provided with a limiting bulge matched with the notch so that the first magnetic attraction element can rotate along with the rotating shaft.

Among the above-mentioned technical scheme, the component can cup joint with the bottom wall portion in piston cavity is inhaled to annular structure's first magnetism to can carry out bottom ring to the magnetic bead in the piston cavity and inhale, adsorption area is bigger, has improved the adsorption effect to the magnetic bead, avoids appearing adsorbing the magnetic bead completely the minimum in piston cavity and easily along with the phenomenon of solution discharge in the lump. And through set up jaggedly on first magnetism inhale the component, breach and the spacing protruding cooperation of passageway are inhaled to first magnetism, spacing arch can inhale the component to first magnetism and carry on spacingly, prevents that there is relative rotation between component and the pivot is inhaled to first magnetism to make first magnetism inhale the component can rotate along with the pivot is synchronous.

In some embodiments, the magnetic attraction device further comprises a side attraction component, and the side attraction component is arranged on the outer side surface of the elution cavity of the gene detection kit and used for magnetically attracting and positioning the magnetic beads in the elution cavity.

Among the above-mentioned technical scheme, through being equipped with the side suction subassembly at the lateral surface of kit, the side suction subassembly mainly adsorbs the lateral surface in the elution chamber of gene assay kit to can make the magnetic bead stop in the elution intracavity.

In a second aspect, an embodiment of the present application further provides a gene detection apparatus, where the gene detection apparatus includes a gene detection kit and the aforementioned magnetic attraction device, the gene detection kit includes a box body and a piston cylinder, a piston cavity is provided in the piston cylinder, a first flow channel communicated with the piston cavity is provided on an outer circumferential surface of the piston cylinder, the box body has an accommodation cavity and a plurality of reagent cavities, the piston cylinder is rotatably provided in the accommodation cavity, a plurality of second flow channels are provided on an inner wall of the accommodation cavity, and each second flow channel is correspondingly communicated with one reagent cavity; the rotating shaft in the magnetic absorption device is used for driving the piston cylinder to rotate in the accommodating cavity, so that the first flow passage can be alternatively communicated with the plurality of second flow passages.

In some embodiments, the piston cylinder comprises a cylinder body and a partition part, the partition part is arranged in the cylinder body and divides the inner space of the cylinder body into a piston cavity and a second magnetic suction channel for the bottom suction assembly to extend into, and the piston cavity and the second magnetic suction channel are arranged along the axial direction of the cylinder body; when the bottom suction assembly is at the first position, the bottom suction assembly is attached to the separating part; when the bottom suction assembly is in the second position, the bottom suction assembly is separated from the partition.

Among the above-mentioned technical scheme, through the setting of partition portion in the piston cylinder for the piston cylinder is formed with the confession and inhales the second magnetism that the subassembly stretched into at the bottom and inhales the passageway, and the second magnetism inhale the outer wall of passageway can with be connected between the pivot, and inhale the subassembly at the bottom and inhale when inhaling the subassembly and inhale magnetism, inhale the subassembly at the bottom and can stretch out outside first magnetism inhales the passageway, and get into the second magnetism and inhale in the passageway, can contact with the bottom in piston chamber, and inhale at the bottom, ensure the end to the magnetic bead and inhale the effect.

Additional features and advantages of the present application will be described in detail in the detailed description which follows.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a schematic structural view of a magnetic attraction device in cooperation with a gene detection kit according to some embodiments of the present disclosure;

FIG. 2 is a schematic view of the magnetic attraction device of FIG. 1 in cooperation with a piston cylinder;

FIG. 3 is a cross-sectional view A-A of FIG. 2;

FIG. 4 is a schematic structural view of a rotating shaft coupled to a bottom suction assembly of the magnetic suction device according to some embodiments of the present disclosure;

FIG. 5 is a schematic structural view of the spindle of FIG. 4;

FIG. 6 is a schematic structural view of the piston cylinder of FIG. 2;

FIG. 7 is a cross-sectional view taken along line B-B of FIG. 6;

FIG. 8 is a schematic view of the gene testing kit of FIG. 1 in combination with a side-draw assembly;

fig. 9 is a cross-sectional view of C-C in fig. 8.

Icon: 10-a rotating shaft; 11-a first magnetic attraction channel; 12-a limit protrusion; 20-a bottom suction assembly; 21-a first magnetically attractive element; 210-a gap; 22-a first connection seat; 220-a slide hole; 23-a second connection seat; 24-an elastic member; 25-a guide bar; 30-a drive member; 40-a side suction assembly; 41-a second magnetically attractive element; 50-a base; 300-gene detection kit; 310-a cartridge; 311-a reagent chamber; 312-a second flow channel; 320-a piston cylinder; 321-a cylinder body; 322-a partition; 323-a second magnetic attraction channel; 324-a first flow channel; 325-magnetic bead retention tank; 326-piston cavity.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the embodiments of the present application, it should be noted that the indication of the orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship which is usually placed when the product of the application is used, and is only for the convenience of describing the application and simplifying the description, and does not indicate or imply that the indicated device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the application. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

In the description of the present application, it is also to be noted that, unless otherwise explicitly specified or limited, the terms "disposed" and "connected" are to be interpreted broadly, e.g., as being either fixedly connected, detachably connected, or integrally connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.

Examples

The gene detection is a medical detection technology, which extracts nucleic acid in peripheral venous blood, tissues and other body fluids of a detected person, analyzes DNA molecule or RNA molecule information in cells of the detected person through a detection device, so as to know the gene information of the detected person and further determine the cause of disease or the risk of disease. The nucleic acid extraction is a pretreatment process of gene detection, specific primers and probe design are carried out according to the sequence of known nucleic acid, the designed primers are synthesized, the extracted nucleic acid is used as a template to carry out a fluorescence quantitative PCR experiment, and the negative and positive of a target sample are judged according to a fluorescence signal. Nucleic acid extraction is a key step of gene detection, and the quality of the obtained nucleic acid directly influences the success or failure of downstream experiments. It should be understood that the gene detection is not limited to the detection of human body, but also includes the gene detection of animal and plant.

The embodiment of the application provides gene detection equipment which can be applied to a nucleic acid extraction link in gene detection. Referring to fig. 1, 2 and 3, the gene testing apparatus includes a gene testing kit 300 and a magnetic attraction device, the gene testing kit 300 includes a box body 310 and a piston cylinder 320, a piston cavity 326 is provided in the piston cylinder 320, a first flow channel 324 communicated with the piston cavity 326 is provided on the outer circumferential surface of the piston cylinder 320, the box body 310 has an accommodation cavity and a plurality of reagent cavities 311, the piston cylinder 320 is rotatably provided in the accommodation cavity, a plurality of second flow channels 312 are provided on the inner wall of the accommodation cavity, and each second flow channel 312 is correspondingly communicated with one reagent cavity 311; the shaft 10 (shown in fig. 4) of the magnetic attraction device is used to drive the piston cylinder 320 to rotate in the accommodating cavity, so that the first flow channel 324 can be alternatively communicated with the plurality of second flow channels 312, and the magnetic attraction device can cooperate with the gene detection kit 300 to magnetically attract the same.

Referring to fig. 2, fig. 3 and fig. 4, the magnetic attraction device includes a rotating shaft 10, a bottom attraction component 20 and a driving component 30, the rotating shaft 10 is used for cooperating with a piston cylinder 320 in the gene detection kit 300 to drive the piston cylinder 320 to rotate, and a first magnetic attraction channel 11 is disposed inside the rotating shaft 10 along the axial direction thereof; the bottom suction assembly 20 is used for magnetically sucking and positioning magnetic beads in the piston cavity of the piston cylinder 320, and the bottom suction assembly 20 is movably arranged in the first magnetic sucking channel 11 and has a first position and a second position; when the bottom suction assembly 20 is in the first position, the bottom suction assembly 20 can be attached to the bottom of the piston cavity 326 to magnetically attract and position the magnetic beads in the piston cavity 326; when the bottom suction assembly 20 is in the second position, the bottom suction assembly 20 is separated from the bottom of the piston cavity 326; the driving member 30 is used for driving the bottom suction assembly 20 to switch between the first position and the second position.

In this scheme, the bottom of the piston cylinder 320 in the gene detection kit 300 needs to cooperate with the pivot 10, thereby make the piston cylinder 320 rotate, thereby realize the reagent exchange in the gene detection kit 300, consequently make arranging of end-sucking subassembly 20 comparatively difficult, and in this scheme through with end-sucking subassembly 20 integration in pivot 10, make it realize the magnetic bead in the minimum space in pivot 10 and adsorb the function, make gene detection equipment's compact structure better, more do benefit to the space overall arrangement of magnetism device in gene detection equipment, improve gene detection equipment's whole compactness. Under the effect of driving piece 30, the end is inhaled subassembly 20 and can be removed in the passageway 11 is inhaled to first magnetism in pivot 10, and the end is inhaled subassembly 20 and can be removed to the bottom in piston chamber, carries out magnetism to the magnetic bead in the piston chamber 326 and inhale the location, and the magnetic bead of being convenient for stops in the piston cylinder 320 to with the separation of the reagent liquid in the piston cylinder 320, let reagent liquid return to corresponding reagent chamber 311.

It should be noted that, in the nucleic acid extraction process, the reagent chamber 311 in the gene detection kit 300 is divided according to functions, and may generally include a lysis chamber, a washing chamber, an elution chamber, and a product output chamber. Each second flow channel 312 is communicated with one reagent cavity 311, which only means that each second flow channel 312 is communicated with the corresponding reagent cavity 311 when being communicated with the first flow channel 324, and is not limited to that each second flow channel 312 must be communicated with the corresponding reagent cavity 311 at any moment. In particular, the second channel 312 corresponding to the product output cavity may be connected to the product output cavity at any time through the PCR reaction tube, or the second channel 312 may be connected to the product output cavity through the PCR reaction tube only when the second channel 312 is connected to the first channel 324, and the second channel 312 is not connected to the product output cavity when the second channel 312 is not connected to the first channel 324.

The driving member 30 may be a variety of driving mechanisms, such as a telescopic cylinder, a fixed shaft motor, a cam mechanism, or other driving mechanisms with lifting function. In the present embodiment, the driving member 30 may be a fixed shaft type motor.

In some embodiments, when the bottom suction assembly 20 is in the first position, the bottom suction assembly 20 at least partially protrudes out of the first magnetic attraction channel 11; when the bottom suction assembly 20 is at the second position, the bottom suction assembly 20 is retracted into the first magnetic attraction channel 11.

Inhale subassembly 20 through end and can stretch out outside first magnetism is inhaled passageway 11, inhale subassembly 20 at end like this and can inhale the piston chamber that is located the gene assay kit 300 outside first magnetism is inhaled passageway 11 and inhale, even piston cylinder 320 bottom can not contact with pivot 10, inhale subassembly 20 at the same end and also can stretch out outside first magnetism is inhaled passageway 11, improved the stroke of inhaling subassembly 20 at the end in first magnetism is inhaled passageway 11, and application scope is wider.

In some embodiments, the bottom suction assembly 20 includes a first magnetic element 21, a first connection seat 22, a second connection seat 23, and a resilient member 24, the first magnetic element 21 is used for magnetically positioning magnetic beads in the piston cavity; the first connecting seat 22 is used for installing the first magnetic attraction element 21; the second connecting seat 23 is used for connecting with the driving piece 30; the elastic member 24 is connected between the first connecting seat 22 and the second connecting seat 23.

Be equipped with elastic component 24 between first connecting seat 22 and second connecting seat 23, when driving piece 30 drove first magnetism and inhale component 21 upward movement, elastic component 24 is owing to have buffering and deformation effect, and elastic component 24 can avoid leading to the problem of the overstroke of first magnetism and inhale component 21 or owe the stroke because of manufacturing, installation error to the elastic force of elastic component 24 can make end inhale subassembly 20 and hug closely with the bottom of piston cavity 326, has improved the magnetism effect of inhaling subassembly 20 at the bottom.

Wherein the resilient member 24 may be a compression spring, such as a metal compression spring or a plastic compression spring. The elastic member 24 may be an air spring formed by a cylinder, a piston rod, or the like, or even a rubber member having a good elastic restoring force.

In some embodiments, a sliding hole 220 is formed in the first connecting seat 22, one end of the first connecting seat 22 is connected to the first magnetic element 21, the other end of the first connecting seat 22 is slidably sleeved on the second connecting seat 23 through the sliding hole 220, and the elastic element 24 is disposed in the sliding hole 220.

Through having set up slide opening 220 in first connecting seat 22, slide opening 220 can lead the slip between first connecting seat 22 and the second connecting seat 23 to also restricted the flexible direction of elastic component 24, ensured the stability of first magnetism component 21 removal process.

In some embodiments, the bottom suction assembly 20 further includes a guide rod 25, the guide rod 25 extends along the axial direction of the rotating shaft 10, the first connecting seat 22 is slidably sleeved on the guide rod 25, one end of the guide rod 25 is connected to the second connecting seat 23, the other end of the guide rod 25 is provided with a limiting portion for limiting the first connecting seat 22 to be separated from the second connecting seat 23, and the elastic member 24 is sleeved on the guide rod 25.

Guide bar 25 can play the effect of connecting first connecting seat 22 and second connecting seat 23 to wear to establish the guide effect for elastic component 24 provides, spacing portion can restrict first connecting seat 22 and break away from second connecting seat 23, improves the stability of inhaling subassembly 20 when removing at the bottom.

The guide rod 25 may be of various structures, such as a pin, a screw, etc.

In some embodiments, the first magnetic element 21 is magnetically connected with the first connecting seat 22. The first magnetic attraction element 21 and the first connecting seat 22 are tightly attracted through magnetic force, so that the connection between the first magnetic attraction element 21 and the first connecting seat 22 can be realized, the first magnetic attraction element 21 and the first connecting seat 22 can relatively rotate without vertical relative movement, when the rotating shaft 10 drives the piston cylinder to rotate to switch the flow channel, the first magnetic attraction element 21 cannot relatively rotate with the rotating shaft 10, and the rotating shaft 10 can be rotated along with the rotating shaft.

In some embodiments, referring to fig. 4 and 5, the first magnetic attraction element 21 is a ring-shaped structure for attracting magnetic beads in the piston cavity 326 when the bottom suction assembly 20 is in the first position; the first magnetic element 21 has a notch 210 formed along the axial direction thereof, and the inner wall of the first magnetic passage 11 has a limiting protrusion 12 matching with the notch 210, so that the first magnetic element 21 can rotate along with the rotation shaft.

The first magnetism of loop configuration is inhaled component 21 and can be cup jointed with the bottom wall portion of piston cavity 326 to can carry out bottom ring to the magnetic bead in the piston cavity 326 and inhale, adsorption area is bigger, has improved the adsorption effect to the magnetic bead, avoids appearing adsorbing the magnetic bead completely at the minimum of piston cavity 326 and easily along with the phenomenon of solution discharge in the lump. And through seted up breach 210 on first magnetism is inhaled component 21, breach 210 and the spacing arch 12 cooperation of first magnetism passageway 11, spacing arch 12 can be inhaled component 21 to first magnetism and spacing, prevents to have relative rotation between first magnetism and inhale component 21 and the pivot 10 to make first magnetism inhale component 21 can rotate along with pivot 10 synchronization.

Specifically, the first magnetic attraction element 21 may be a magnetic rod, the magnetic rod is a permanent magnet, and the magnetic rod is C-shaped.

In addition, it should be noted that, under the condition that the first magnetic element 21 rotates synchronously along with the rotating shaft 10, the limiting protrusion 12 in the rotating shaft 10 may coincide with the first flow channel 324 of the piston cavity 326 in the projection of the thickness direction of the piston cylinder 320, and when the first magnetic element 21 is at the first position, the notch 210 of the first magnetic element 21 is engaged with the first flow channel 324 at the bottom of the piston cavity 326, so that the magnetic force generated by the first magnetic element 21 and the first magnetic element 21 may be exactly dislocated with the first flow channel 324, and the first magnetic element 21 adsorbs the magnetic beads in the piston cavity 326 to other parts except the first flow channel 324, thereby preventing the reagent liquid in the first flow channel 324 in the piston cavity 326 from bringing the magnetic beads into the reagent cavity 311 together when flowing, and improving the adsorption effect on the magnetic beads.

In some embodiments, referring to fig. 6 and 7, the piston cylinder 320 includes a cylinder body 321 and a partition portion 322, the partition portion 322 is disposed in the cylinder body 321 and divides an inner space of the cylinder body 321 into a piston cavity 326 and a second magnetic suction channel 323 into which the bottom suction assembly 20 extends, and the piston cavity 326 and the second magnetic suction channel 323 are arranged along an axial direction of the cylinder body 321; when the bottom suction assembly 20 is at the first position, the bottom suction assembly 20 is attached to the partition 322; when the bottom suction assembly 20 is in the second position, the bottom suction assembly 20 is separated from the partition 322.

Through the setting of partition portion 322 in the piston cylinder 320, make the piston cylinder 320 be formed with the confession and inhale the second magnetism passageway 323 of inhaling that subassembly 20 stretched into at the bottom, the second magnetism inhale the outer wall of passageway 323 can with be connected between the pivot 10, and inhale when subassembly 20 is inhaled at the bottom and inhale the magnetism, inhale the subassembly 20 at the bottom and can stretch out outside first magnetism passageway 11 of inhaling, and go into the second magnetism and inhale the passageway 323 in, can be with the bottom contact of piston chamber 326, promptly with partition portion 322 contact, and carry out the end to the magnetic bead and inhale, ensure the end to the magnetic bead and inhale the effect.

In some embodiments, the bottom surface of the piston cavity 326 is inclined from the outer periphery to the middle, the inclined bottom surface of the piston cavity 326 is provided with a magnetic bead retention groove 325 for containing magnetic beads, and the magnetic bead retention grooves 325 are distributed on the bottom surface of the piston cavity 326 in a ring shape; when the first magnetic element 21 is at the first position, the top surface of the first magnetic element 21 and the surface of the magnetic bead retention groove 325 are on the same plane.

Be provided with the magnetic bead through the bottom surface at piston cavity 326 and detain groove 325, magnetic bead detains groove 325 can be used for saving the magnetic bead, when the piston slowly pushes down in piston cavity 326, other cavities are injected into to the solution in the piston cavity 326, and magnetic bead detains groove 325 can provide the effect of detaining to the magnetic bead, lets the magnetic bead stop in magnetic bead detains groove 325 and is inhaled component 21 by first magnetism, and the magnetic bead is not taken away by solution, can improve the adsorption efficiency to the magnetic bead.

Specifically, the inner diameter of the first magnetic element 21 can match the outer diameter of the surface where the magnetic bead retention groove 325 is located on the bottom surface of the piston cavity 326, so that the top surface of the first magnetic element 21 can be flush with the surface where the magnetic bead retention groove 325 is located, and the magnetic bead adsorption effect in the magnetic bead retention groove 325 is better.

In some embodiments, the axis of the piston cavity 326 is coaxially disposed with the first magnetically attractive element 21. Through first magnetism inhale component 21 and wait that the regional piston cavity of adsorbing distributes for coaxial axle center, first magnetism inhale the component 21 more even in the magnetic field distribution of piston cavity 326 bottom, the distribution of magnetic bead in piston cavity 326 is more even so to the adsorption effect to the magnetic bead is better.

In some embodiments, referring to fig. 8 and fig. 9, the magnetic attraction device further includes a side attraction component 40, and the side attraction component 40 is disposed on an outer side surface of an elution cavity of the gene detection kit 300 and is used for magnetically attracting and positioning magnetic beads in the elution cavity. Through being equipped with side suction assembly 40 at the lateral surface of gene detect reagent box 300, side suction assembly 40 mainly adsorbs the lateral surface of the elution chamber of gene detect reagent box 300 to can make the magnetic bead stay in the elution intracavity.

Specifically, when the elution chamber of gene detection kit 300 is in elution process, the magnetic bead is adsorbed once by side-suction assembly 40 in the elution chamber of gene detection kit 300 earlier, let most magnetic beads stay in the elution chamber, treat that solution shifts to behind piston cavity 326, then adopt end suction assembly 20 to adsorb once more to part along with the magnetic bead that the eluant flowed into in piston cavity 326 again, thereby use end suction assembly 20 to carry out the secondary to remaining magnetic bead in the piston cavity 326 and adsorb, can realize the complete absorption to the magnetic bead in the solution basically, avoid the magnetic bead to enter into subsequent extraction process, influence the operation of extracting of nucleic acid. Therefore, the adoption of the side-suction assembly 40 and the bottom-suction assembly 20 for two times of adsorption can improve the purity of the nucleic acid, and avoid the influence on the subsequent detection result of the nucleic acid due to insufficient adsorption of the magnetic beads.

Wherein, the subassembly 40 is inhaled to side can include that the second is inhaled the magnetism and is inhaled component 41, and the second is inhaled the component 41 to magnetism and has the binding face that matches with the radian in gene detection kit 300's elution chamber, and the binding face can contact with the side in elution chamber, compares in planar binding face, and the area of contact of the side in component 41 and elution chamber is inhaled to curved binding face can increase the second magnetism to ensure that the second is inhaled the component 41 and is inhaled the side of the magnetic bead of elution intracavity in the kit.

In addition, the second magnetic attraction element 41 may be a permanent magnet or an electromagnet. When the second magnetic attraction element 41 is an electromagnet, the electromagnet on the side of the elution chamber controls the presence or absence of the magnetic field in an off/on manner, thereby controlling the side attraction. Of course, when the second magnetic attraction element 41 is a permanent magnet, an external force can be used to drive the permanent magnet to be close to/far from the side wall of the elution chamber. In this embodiment, component 41 adopts for the electro-magnet is inhaled to the second magnetism, compares in conventional permanent magnet, and the electro-magnet circular telegram produces the magnetic field, adsorbs the magnetic bead in the elution chamber, adsorbs the completion back, and the electro-magnet outage, magnetic bead adsorb the end, and the electro-magnet need not remove to be close to or keep away from the elution chamber, only needs the circular telegram and the outage of control electro-magnet, and control mode is simple and convenient to practice thrift occupation space on the base 50.

In some embodiments, the magnetic attraction device may further include a driving mechanism (not shown in the drawings), the driving mechanism may drive the rotating shaft to rotate, the driving mechanism may include a driving motor and a transmission member, an output end of the driving motor is connected to the transmission member, and the transmission member is in transmission connection with the rotating shaft.

The process of nucleic acid extraction by the magnetic bead method is generally divided into:

cracking: the process of breaking cell through enzyme catalytic reaction or mechanical oscillation to release the genetic matter in the cell nucleus.

Combining: a specific reagent environment is created by adding a binding reagent, so that the magnetic beads and the DNA are specifically bound;

washing: through adding the washing liquid, add mechanical stirring action for the magnetic bead that has adsorbed DNA and impurity is fully mixed with the washing liquid, and in this process, impurity can dissolve in the washing liquid, and DNA can not separate from the magnetic bead.

And (3) elution: by adding the eluent, a specific reagent environment is created, so that the DNA can be separated from the magnetic beads and dissolved in the eluent to obtain a pure DNA solution.

It should be noted that the features of the embodiments in the present application may be combined with each other without conflict.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (10)

1. The utility model provides a device is inhaled to magnetism for cooperate with gene testing kit, its characterized in that includes:

the rotating shaft is used for being matched with a piston cylinder in the gene detection kit to drive the piston cylinder to rotate, and a first magnetic suction channel is arranged in the rotating shaft along the axial direction of the rotating shaft;

the bottom suction assembly is used for carrying out magnetic attraction positioning on magnetic beads in the piston cavity of the piston cylinder, is movably arranged in the first magnetic attraction channel and is provided with a first position and a second position; when the bottom suction assembly is located at the first position, the bottom suction assembly can be attached to the bottom of the piston cavity so as to perform magnetic attraction positioning on magnetic beads in the piston cavity; when the bottom suction assembly is in the second position, the bottom suction assembly is separated from the bottom of the piston cavity;

the driving piece is used for driving the bottom suction assembly to be switched between the first position and the second position.

2. The magnetic attraction device of claim 1, wherein when the bottom suction assembly is in the first position, the bottom suction assembly extends at least partially out of the first magnetic attraction channel; when the bottom suction assembly is at the second position, the bottom suction assembly retracts into the first magnetic suction channel.

3. The magnetic attraction device of claim 1 wherein the bottom suction assembly comprises:

the first magnetic attraction element is used for magnetically attracting and positioning magnetic beads in the piston cavity;

the first connecting seat is used for mounting the first magnetic suction element;

the second connecting seat is used for being connected with the driving piece;

the elastic piece is connected between the first connecting seat and the second connecting seat.

4. The magnetic attraction device of claim 3, wherein the first connecting seat has a sliding hole therein, one end of the first connecting seat is connected to the first magnetic attraction element, the other end of the first connecting seat is slidably sleeved on the second connecting seat through the sliding hole, and the elastic member is disposed in the sliding hole.

5. The magnetic attraction device of claim 4 wherein the bottom suction assembly further comprises:

the guide rod extends along the axial direction of the rotating shaft, the first connecting seat is slidably sleeved on the guide rod, one end of the guide rod is connected to the second connecting seat, the other end of the guide rod is provided with a limiting portion, the limiting portion is used for limiting the first connecting seat to be separated from the second connecting seat, and the elastic piece is sleeved on the guide rod.

6. The magnetic attraction device of claim 3 wherein the first magnetic element is magnetically coupled to the first mount.

7. The magnetic attraction device of claim 3, wherein the first magnetic attraction element is of an annular configuration for attracting magnetic beads in the piston chamber when the bottom suction assembly is in the first position; the first magnetic attraction element is provided with a notch along the axial direction in a penetrating mode, and the inner wall of the first magnetic attraction channel is provided with a limiting protrusion matched with the notch, so that the first magnetic attraction element can rotate along with the rotating shaft.

8. The magnetic attraction device of any one of claims 1-7 further comprising:

and the side suction component is arranged on the outer side surface of an elution cavity of the gene detection kit and is used for carrying out magnetic suction positioning on magnetic beads in the elution cavity.

9. A gene assaying device characterized by comprising:

the gene detection kit comprises a kit body and a piston cylinder, wherein a piston cavity is arranged in the piston cylinder, a first flow channel communicated with the piston cavity is arranged on the outer peripheral surface of the piston cylinder, the kit body is provided with an accommodating cavity and a plurality of reagent cavities, the piston cylinder is rotatably arranged in the accommodating cavity, a plurality of second flow channels are arranged on the inner wall of the accommodating cavity, and each second flow channel is correspondingly communicated with one reagent cavity;

the magnetic attraction device of any one of claims 1-8, wherein the shaft is configured to drive the piston cylinder to rotate within the chamber to alternatively communicate the first flow passage with the plurality of second flow passages.

10. The gene assaying device according to claim 9,

the piston cylinder comprises a cylinder body and a partition part, the partition part is arranged in the cylinder body and divides the inner space of the cylinder body into a piston cavity and a second magnetic suction channel for the bottom suction assembly to extend into, and the piston cavity and the second magnetic suction channel are arranged along the axial direction of the cylinder body;

when the bottom suction assembly is at the first position, the bottom suction assembly is attached to the separating part; when the bottom suction assembly is in the second position, the bottom suction assembly is separated from the partition.

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