CN214174395U - Micro-reactor - Google Patents

Abstract

The utility model discloses a micro-reactor, including reactor body and base, reactor body bottom is installed on the base, and is provided with the one deck filter membrane between reactor body and the base, the aperture that can supply negative pressure extraction liquid is seted up to the bottom of base, this aperture and reactor body intercommunication. When the waste liquid of extraction, take out negative pressure structure and can follow the aperture of base and take out the waste liquid, and the magnetic bead in the reactor then can be kept apart in the filter membrane top by the filter membrane, avoids the magnetic bead to run off.

Description

Micro-reactor

Technical Field

The utility model belongs to the technical field of biochip, concretely relates to microreactor.

Background

At present, most of reactions of biochips are carried out by using magnetic beads. Firstly, a target substance is specifically bonded on magnetic beads, then impurities on the magnetic beads are cleaned, waste liquid is discharged, only the cleaned magnetic beads are left, and finally, the substances on the magnetic beads are detected. Therefore, the magnetic beads need to be prevented from being lost during the process of removing the waste liquid.

Most of the magnetic beads are magnetic beads which can be attracted by the magnet, and the waste liquid can be discharged by adopting a mode of firstly attracting the magnetic beads and then discharging the waste liquid. Some magnetic beads are plastics or glass materials, and this kind of magnetic bead can't be adsorbed by magnet, can only realize the reservation of magnetic bead and the emission of waste liquid through filterable mode, then need consider how to prevent that the magnetic bead from losing this moment

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a micro-reactor to solve the problem that the magnetic bead runs off when extracting the waste liquid.

In order to achieve the above object, the utility model adopts the following technical scheme: a micro-reactor comprises a reactor body and a base, wherein the bottom of the reactor body is arranged on the base, a layer of filter membrane is arranged between the reactor body and the base, and the bottom of the base is provided with a small hole for extracting liquid under negative pressure, and the small hole is communicated with the reactor body.

When the waste liquid after the extraction reaction, take out negative pressure structure and can follow the aperture of base and take out the waste liquid, and the magnetic bead in the reactor then can be kept apart in the filter membrane top by the filter membrane, avoids the magnetic bead to run off.

Preferably, the reactor body is in interference fit connection with the base, so that a bonding process and bonding equipment cost are avoided. The production efficiency is improved.

Preferably, the caliber of the upper part of the reactor body is larger than that of the lower part. This kind of back taper design can realize the automatic application of sample of the reagent of prestoring in the chip, avoids realizing the application of sample through application of sample needle and reagent bottle like traditional detecting instrument.

Preferably, the center of the top of the reactor body is provided with a window for sampling detection. The window is arranged to facilitate the sample needle to be inserted into the center of the reactor, so as to ensure the uniformity of the substance to be detected absorbed by the sample needle.

Preferably, the window diameter is 4-6 mm, and the window is matched with the size of a corresponding detection instrument.

Preferably, the diameter of the small hole is 0.1-0.5 mm. The small hole of 0.1-0.5 mm can ensure that the liquid in the reactor can not flow out under normal conditions and can ensure that the liquid is discharged under the condition of negative pressure.

Preferably, both sides of the base are provided with connecting lugs.

The utility model has the advantages that:

1. the utility model can simply realize the reaction and uniform mixing for the magnetic bead reaction, and the waste liquid is pumped away by negative pressure, no magnetic bead loss exists, and the repeatability is good;

2. the reactor body and the base are sealed through interference assembly, the production and assembly are simple, and the efficiency is high;

3. the utility model discloses simple structure, it is automatic easily to form a complete set instrument realization.

Drawings

Fig. 1 is an exploded view of the present invention;

FIG. 2 is an assembly view of the present invention;

FIG. 3 is a top view of FIG. 2;

FIG. 4 is a bottom view of FIG. 2;

fig. 5 is a schematic view of the present invention in use.

Detailed Description

The present invention will be further explained with reference to the drawings and examples.

As shown in figures 1-5, a micro-reactor is assembled by a reactor body 1 with an open bottom, a filter membrane 2 and a base 3. The reactor body 1 and the base 3 are sealed through interference fit, so that a bonding process and bonding equipment cost can be saved. The base 3 is provided with a small hole 4, and the diameter of the small hole 4 is preferably 0.1-0.5 mm so as to realize negative pressure waste liquid extraction.

Reactor body 1 adopts the back taper design, and the bore on its upper portion is greater than the bore of lower part, can realize the automatic application of sample of 8 pre-storage reagents in the application of sample position in the chip, and the biochip is advanced in just simple integration of reactor ability like this, and better realization is automatic, avoids like traditional detecting instrument needing to realize the application of sample through application of sample needle and reagent bottle. The reactor body 1 is of an integral injection molding structure, has no bonding joint surface, and has a hydrophobic and smooth surface and less residue. A window 5 with the diameter of 4-6 mm for realizing sampling detection is reserved at the center of the top of the reactor body 1, and a sample needle at a sampling detection position 7 in a chip can be inserted into the center of the reactor to absorb substances to be detected. The two sides of the base 3 are provided with connecting lugs 6 which provide a connecting interface for the reactor. When the base 3 is installed, the shaft on the chip passes through the through hole of the connecting lug 6 to be in interference connection with the connecting lug.

The blending reaction of the reactor is realized by ultrasound. When negative pressure extraction waste liquid, because negative pressure extraction waste liquid, base 3 can realize with taking out negative pressure structure contactless, simple structure, place also not have the requirement to the chip.

Claims (7)

1. The micro-reactor is characterized by comprising a reactor body (1) and a base (3), wherein the bottom of the reactor body (1) is arranged on the base (3), a layer of filter membrane (2) is arranged between the reactor body (1) and the base (3), a small hole (4) for extracting liquid under negative pressure is formed in the bottom of the base (3), and the small hole (4) is communicated with the reactor body (1).

2. Microreactor according to claim 1, wherein the reactor body (1) is connected to the base (3) by interference.

3. Microreactor according to claim 1, characterized in that the caliber of the upper part of the reactor body (1) is larger than the caliber of the lower part.

4. The microreactor according to claim 1 wherein the reactor body (1) is centrally provided with a window (5) for sampling detection.

5. The microreactor according to claim 4 wherein the window (5) has a diameter of 4 to 6 mm.

6. The microreactor according to claim 1 wherein the diameter of the small holes (4) is 0.1 to 0.5 mm.

7. Microreactor according to claim 1, wherein the base (3) is provided with coupling lugs (6) on both sides.

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