CN215404665U - Capture and library building device - Google Patents

Abstract

The utility model provides a device for capturing and building a library, which comprises: the device body is provided with a mounting position; the kit is arranged at the installation position; the kit comprises a first kit body and a second kit body detachably connected to the first kit body, wherein the first kit body is provided with a mounting hole; the liquid transferring mechanism comprises a rotating assembly, a liquid transferring assembly and a driving assembly; one end of the liquid transfer assembly is positioned in the first middle through hole and is communicated with the liquid transfer device, and the other end of the liquid transfer assembly is suspended in the first kit body or the second kit body; the driving assembly is used for driving the rotating assembly to rotate around the mounting hole by 360 degrees, and the driving assembly is used for driving the pipetting assembly to stretch into or draw out the first kit body or the second kit body. According to the utility model, the technical problem of low efficiency of the traditional capturing and building device is solved by optimally setting the specific structure of the capturing and building device.

Description

Capture and library building device

Technical Field

The utility model relates to the technical field of molecular biology, in particular to a capture and library building device.

Background

The targeted sequence capture technology is mainly based on probe hybridization capture technology or multiple PCR. The probe hybridization capture technology is that a specific probe is synthesized on a solid phase or liquid phase chip, and a target gene sequence is captured from a genome library. The anchored multiplex PCR technology is based on PCR, and adds a plurality of pairs of primers in the same PCR reaction system to simultaneously amplify the PCR reaction of a plurality of target gene sequences.

The library construction refers to the construction of a genetic information library of a certain targeting sequence. The library building (library preparation) process involves the process links of adding a gene sample, adding a buffer solution and enzyme reagent mixed solution, PCR (polymerase chain reaction), magnetic bead purification and the like, the process is complex, the used liquid system is huge, and the risks of sample mixing and sample pollution are extremely high.

At present, the traditional storehouse building all-in-one machine has no capture function and is large in size. For the experiment needing to be captured, the capturing experiment process needs to be carried out independently, so that the whole capturing and warehouse building operation is complex in operation and low in efficiency; meanwhile, due to the influence of the receptor volume, the use environment space is limited, the method is only suitable for experimental library building of large-batch samples and is not suitable for small-volume and small-flux samples, and the universality of the traditional library building all-in-one machine is low.

SUMMERY OF THE UTILITY MODEL

Aiming at the defects in the prior art, the utility model provides a capture and library building device, which aims to solve the technical problem that the traditional library building integrated device in the related art is low in efficiency and universality.

The utility model provides a device for capturing and building a library, which comprises:

the device body is provided with a mounting position;

the kit is arranged at the installation position; the kit is used for capturing the target gene of library construction; the kit comprises a first kit body and a second kit body detachably connected to the first kit body, wherein the first kit body is provided with a mounting hole; the first kit body is used for loading a normal-temperature sequencing sample liquid, and the second kit body is used for loading a sequencing sample liquid to be refrigerated;

the liquid transferring mechanism comprises a rotating assembly, a liquid transferring assembly and a driving assembly; the rotating assembly is rotatably arranged in the mounting hole and is provided with a first through hole penetrating through the rotating assembly, and the first through hole extends along the axial direction of the rotating assembly; one end of the liquid transfer assembly is positioned in the first middle through hole and is communicated with the liquid transfer device, and the other end of the liquid transfer assembly is exposed out of the first middle through hole and is suspended in the first kit body or the second kit body; the driving assembly is arranged on the device body and used for driving the rotating assembly to rotate around the mounting hole by 360 degrees, and the driving assembly is used for driving the pipetting assembly to stretch into or draw out the first kit body or the second kit body.

Optionally, the first kit body comprises a first console and a sink structure, the sink structure is concavely arranged on the first console, and a second through hole assembly penetrating through the first console is arranged in the sink structure;

the second kit body comprises a second operation table matched with the sinking groove structure, and the second operation table is detachably connected with the sinking groove structure;

the mounting hole is formed in the first operating platform.

Optionally, the first console is provided with a first through hole assembly penetrating through the first console, the first through hole assembly and the second through hole assembly are arranged at intervals, the first kit body further comprises a first accommodating assembly, the first accommodating assembly is provided with an open end and a first accommodating cavity, the open end of the first accommodating assembly is connected to the first console, and the open end of the first accommodating assembly is communicated with the first through hole assembly to form a channel for inserting or extracting the first accommodating cavity;

the second operation platform is provided with a third through hole assembly which penetrates through the second operation platform, the third through hole assembly is matched with the second through hole assembly, the second kit body further comprises a second containing assembly, the second containing assembly is provided with an opening end and a second containing cavity, the opening end of the second containing assembly is connected to the second operation platform, and the opening end of the second containing assembly is communicated with the third through hole assembly to form a channel for inserting or extracting the second containing cavity;

the driving assembly drives the liquid-transfering assembly to stretch into or draw out the first accommodating cavity or the second accommodating cavity.

Optionally, the first through hole assembly includes a plurality of first through holes, a plurality of second through holes, and a plurality of third through holes, and the first through holes, the second through holes, and the third through holes have different sizes;

the first through holes, the second through holes and the third through holes are arranged on the first operating platform in a staggered mode at intervals;

the first accommodating assembly comprises a plurality of first accommodating pieces, a plurality of second accommodating pieces and a plurality of third accommodating pieces, the plurality of first accommodating pieces and the plurality of first through holes are arranged in a one-to-one correspondence manner, and the first accommodating pieces are matched with the first through holes; the plurality of second accommodating pieces and the plurality of second through holes are arranged in a one-to-one correspondence manner, and the second accommodating pieces are matched with the second through holes; the plurality of third accommodating pieces and the plurality of third through holes are arranged in a one-to-one correspondence manner, and the third accommodating pieces are matched with the third through holes.

Optionally, the second through hole assembly comprises a plurality of fourth through holes arranged at intervals and a plurality of fifth through holes arranged at intervals, and the sizes of the fourth through holes and the fifth through holes are different;

the second accommodating assembly comprises a plurality of fourth accommodating pieces and a plurality of fifth accommodating pieces, the fourth accommodating pieces and the fourth through holes are arranged in a one-to-one correspondence manner, and the fourth accommodating pieces are matched with the fourth through holes; the plurality of fifth accommodating parts and the plurality of fifth through holes are arranged in a one-to-one correspondence manner, and the fifth accommodating parts are matched with the fifth through holes.

Optionally, a movable notch is formed in one end, away from the mounting hole, of the rotating assembly, the movable notch extends in the same direction as the first middle through hole, the liquid transfer mechanism further comprises a lifting assembly with a second middle through hole, the second middle through hole extends in the same direction as the first middle through hole, one end of the lifting assembly is rotatably connected to the rotating assembly and located in the first middle through hole, and the other end of the lifting assembly penetrates through the movable notch, is exposed out of the rotating assembly and is suspended above the first kit body or the second kit body;

one end of the liquid transferring assembly is positioned in the second middle through hole and is communicated with the liquid transferring device, and the other end of the liquid transferring assembly is connected to one end, close to the movable notch, of the lifting assembly;

the driving component drives the lifting component to rotate so as to drive the pipetting component to stretch into or draw out the first kit body or the second kit body.

Optionally, the lifting assembly comprises a connecting piece, a threaded piece and a lifting piece, one end of the connecting piece is rotatably connected to the rotating assembly, the other end of the connecting piece is connected to one end of the threaded piece, and the connecting piece and the threaded piece are both located in the first through hole; one end of the lifting piece is rotatably connected to the threaded piece, and the other end of the lifting piece penetrates through the movable notch and is arranged in a suspended mode;

the connecting piece and the threaded piece are both provided with through holes, and the through holes of the connecting piece and the through holes of the threaded piece are communicated to form second middle through holes;

the driving assembly drives the threaded piece to rotate, and the lifting piece abuts against the movable notch so as to drive the lifting piece to reciprocate along the threaded piece.

Optionally, the pipetting assembly comprises a pipette and a TIP head which are communicated, the pipette passes through the second through hole to be connected to the pipettor, and one end of the TIP head is arranged through and fixedly connected to the fixing hole of the lifting piece.

Optionally, the driving assembly includes a first driving member and a second driving member, the first driving member is disposed on the device body, the first driving member is configured to drive the rotating assembly to rotate around the mounting hole by 360 °, and the second driving member is configured to drive the threaded member to rotate.

Optionally, the capturing and library building device further comprises a shell with an open end, wherein the open end of the shell is detachably sleeved on the reagent kit, so that a closed space for sequencing and library building operation is formed between the shell and the reagent kit;

the shell is arranged at the installation position of the device body; the pipetting mechanism is located in the enclosed space.

Compared with the prior art, the utility model has the following beneficial effects:

according to the technology, the specific structure of the capturing and library building device is set in an optimized mode, gene capturing operation and library building operation are achieved integrally through the same device, the operation steps of capturing and library building operation are reduced, and the efficiency of capturing and building a target gene is greatly improved; meanwhile, two sets of target gene capturing and building devices are not needed, so that the space for installing the device is effectively saved, the device for capturing and building the gene tends to be miniaturized and developed, and the universality of the device for capturing and building the gene is improved.

Drawings

Fig. 1 is a schematic structural diagram of a capture library building apparatus according to an embodiment of the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1;

FIG. 3 is an exploded view of FIG. 2;

FIG. 4 is a schematic structural diagram of a kit according to an embodiment of the present invention;

fig. 5 is a schematic perspective view of a housing according to an embodiment of the utility model;

FIG. 6 is an enlarged view of a portion of FIG. 5 after detonation at another viewing angle;

fig. 7 is a partially enlarged view of fig. 6.

The reference numbers illustrate:

the implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.

Detailed Description

In order to make the objects, technical solutions and beneficial effects of the present invention more clearly apparent, the technical solutions of the present invention are further described below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.

Referring to fig. 1 to 7, the present invention provides a capture library building apparatus, including:

a device body 100 having a mounting position;

the kit 200 is arranged at the installation position; the kit 200 is used for capturing the target gene of library construction; the reagent kit 200 comprises a first reagent kit body 210 and a second reagent kit body 220 detachably connected to the first reagent kit body 210, wherein a mounting hole 211 is formed in the first reagent kit body 210; the first kit body 210 is used for loading a normal-temperature sequencing sample liquid, and the second kit body 220 is used for loading a sequencing sample liquid to be refrigerated;

a pipetting mechanism 300 including a rotating assembly 310, a pipetting assembly 320 and a driving assembly (not shown); the rotating member 310 is rotatably mounted in the mounting hole 211, and the rotating member 310 has a first through hole penetrating through the rotating member 310, and the first through hole extends along the axial direction of the rotating member 310; one end of the pipetting assembly 320 is located in the first through hole and is communicated with a pipettor (not shown in the figure), and the other end of the pipetting assembly 320 is exposed out of the first through hole and is suspended in the first kit body 210 or the second kit body 220; the driving component is disposed on the apparatus body 100, and is configured to drive the rotating component 310 to rotate 360 degrees around the mounting hole 211, and drive the pipetting component 320 to extend into or withdraw from the first cartridge body 210 or the second cartridge body 220.

In this embodiment, the reagent kit 200 is provided so that the target gene capturing operation and the library creating operation can be performed in the same apparatus. The reagent cartridge 200 is detachably mounted to the mounting position of the apparatus body 100, so that the reagent cartridge 200 can be conveniently replaced. Specifically, in order to meet the requirements for different reaction sample liquid temperatures, the kit 200 is provided with a first kit body 210 and a second kit body 220 which are detachable, the first kit body 210 for loading a normal-temperature sequencing sample liquid is mounted on the apparatus body 100, and the second kit body 220 for loading a sequencing sample liquid to be refrigerated is detachably mounted on the first kit body 210; therefore, in the transportation process, the first kit body 210 can be transported conventionally, the second kit body 220 is detached and taken out, and is transported in a freezing environment, and the whole using rule is simple and easy to operate.

Meanwhile, a pipetting mechanism 300 is provided to add, aspirate, and transfer the sample solution in the reagent cartridge 200. In order to realize the reaction of each stage on the reagent kit 200 in the capturing and library building operation, the rotating component 310 is rotatably connected to the reagent kit 200, so that the rotating component 310 can rotate 360 degrees around the reagent kit 200, thereby driving the liquid-transferring component 320 to rotate 360 degrees around the reagent kit 200, further realizing the operations of adding, sucking, transferring and the like of sample liquid in different reaction test tubes on the reagent kit 200 by the liquid-transferring component 320, and completing the capturing and library building operation of target genes. Specifically, a rotation member 310 rotatably connected to the reagent cartridge 200, a pipetting member 320 for transferring a sample liquid, and a driving member for driving the rotation member 310 to rotate and the pipetting member 320 to pipette are provided. In order to improve the overall aesthetic appearance, a first through hole is formed in the rotating assembly 310 and used for accommodating the pipetting assembly 320; in this way, the pipetting assembly 320 is accommodated so that the pipetting assembly 320 does not fall out, and the rotating assembly 310 protects the pipetting assembly 320. The setting of drive assembly has effectively improved the intellectuality of whole device and the precision of operation.

According to the technology, the specific structure of the capturing and library building device is set in an optimized mode, gene capturing operation and library building operation are achieved integrally through the same device, the operation steps of capturing and library building operation are reduced, and the efficiency of capturing and building a target gene is greatly improved; meanwhile, two sets of target gene capturing and building devices are not needed, so that the space for installing the device is effectively saved, the device for capturing and building the gene tends to be miniaturized and developed, and the universality of the device for capturing and building the gene is improved.

Optionally, the first cartridge body 210 includes a first console 212 and a sink structure 213, the sink structure 213 is recessed in the first console 212, and a second through hole assembly 214 penetrating through the first console 212 is disposed in the sink structure 213;

the second reagent kit body 220 comprises a second operation platform 221 matched with the sinking groove structure 213, and the second operation platform 221 is detachably connected to the sinking groove structure 213;

the mounting hole 211 is formed in the first operating board 212.

In this embodiment, in order to detachably connect the first cartridge body 210 and the second cartridge body 220, the first cartridge body 210 is configured as a first console 212 for providing a supporting force and a sink structure 213 recessed in the first console 212, and the second cartridge body 220 is configured as a second console 221 for providing a supporting force. The second operation platform 221 may be accommodated in the sinking groove structure 213, and when the second operation platform 221 is accommodated in the sinking groove structure 213, a side of the second operation platform 221 away from the bottom of the sinking groove structure 213 is flush with the top of the first operation platform 212 or a side of the second operation platform 221 away from the bottom of the sinking groove structure 213 is disposed close to the top of the first operation platform 212.

Optionally, a first through hole 2151 assembly 215 penetrating through the first console 212 is disposed on the first console 212, the first through hole 2151 assembly 215 and the second through hole assembly 214 are disposed at intervals, the first cartridge body 210 further includes a first receiving assembly 216, the first receiving assembly 216 has an open end and a first receiving cavity, the open end of the first receiving assembly 216 is connected to the first console 212, and the open end of the first receiving assembly 216 is communicated with the first through hole 2151 assembly 215 to form a passage for inserting or extracting the first receiving cavity;

the second console 221 is provided with a third through hole assembly 222 penetrating through the second console 221, the third through hole assembly 222 is adapted to the second through hole assembly 214, the second kit body 220 further comprises a second accommodating assembly 223, the second accommodating assembly 223 is provided with an open end and a second accommodating cavity, the open end of the second accommodating assembly 223 is connected to the second console 221, and the open end of the second accommodating assembly 223 is communicated with the third through hole assembly 222 to form a channel for inserting or extracting the second accommodating cavity;

the driving assembly drives the liquid-transferring assembly 320 to extend into or withdraw from the first accommodating cavity or the second accommodating cavity.

In this embodiment, in order to load/sequence the normal temperature sample liquid, a first accommodating component 216 is provided. The first receiving member 216 and the sink structure 213 are disposed on opposite sides of the first operating platform 212. For example, but not limiting of, the first receiving assembly 216 is disposed below the first stage 212, and the sink structure 213 is disposed above the first stage 212. The first operating platform 212 is provided with a first through hole 2151 assembly 215 penetrating through the first operating platform 212, the first accommodating assembly 216 is provided with an open end and a first accommodating cavity communicated with the open end, and the open end of the first accommodating assembly 216 is communicated with the first through hole 2151 assembly 215 so as to facilitate the insertion or extraction of a normal-temperature sample liquid into or out of the first accommodating cavity. The first accommodating cavity is used for accommodating a normal-temperature test solution and providing an operation space for normal-temperature sample solution sequencing.

In order to load/sequence the sample liquid to be refrigerated, etc., a second receiving module 223 is provided. The second operation platform 221 is provided with a third through hole assembly 222 penetrating through the second operation platform 221, the second accommodating assembly 223 is provided with an opening end and a second accommodating cavity communicated with the opening end, the opening end of the second accommodating assembly 223 is communicated with the third through hole assembly 222, so that the second accommodating cavity can be conveniently inserted or extracted by refrigerating sample liquid. The second accommodating cavity is used for accommodating the refrigerated test liquid and providing an operation space for sequencing the refrigerated sample liquid.

Specifically, the size of the second through hole component 214 is matched with the size of the second accommodating component 223, so that when the second reagent kit body 220 is installed in the sinking groove structure 213, the second accommodating component 223 passes through the second through hole component 214, and the second operating platform 221 is abutted with the sinking groove structure 213 in a matching manner. So, can be according to actual sequencing needs, nimble installation or take out second kit body 220 to the sample liquid that awaits measuring/need be refrigerated to be measured at the normal atmospheric temperature of sequencing alone, or the sample liquid that awaits measuring and the sample liquid that awaits measuring that needs to be refrigerated at the normal atmospheric temperature of sequencing simultaneously. Thus, the functions of the kit 200 are optimized, and the adaptability of the kit 200 to different sequencing sample liquids is improved; meanwhile, the sample liquid to be tested at normal temperature or needing to be refrigerated is not required to be subjected to sequencing in batches, so that the efficiency of gene sequencing and library building is greatly improved.

Optionally, the first via 2151 assembly 215 includes a plurality of first vias 2151, a plurality of second vias 2152, and a plurality of third vias 2153, the first vias 2151, the second vias 2152, and the third vias 2153 being different in size;

the first through holes 2151, the second through holes 2152, and the third through holes 2153 are alternately disposed on the first operating platform 212;

the first accommodating assembly 216 includes a plurality of first accommodating parts, a plurality of second accommodating parts and a plurality of third accommodating parts, the plurality of first accommodating parts are arranged corresponding to the plurality of first through holes 2151 one by one, and the first accommodating parts are matched with the first through holes 2151; the plurality of second accommodating pieces and the plurality of second through holes 2152 are arranged in a one-to-one correspondence manner, and the second accommodating pieces are matched with the second through holes 2152; the third accommodating pieces and the third through holes 2153 are arranged in a one-to-one correspondence manner, and the third accommodating pieces are matched with the third through holes 2153.

In this embodiment, in order to simultaneously sequence different amounts of sample liquids to be tested, the first accommodating components 216 with different sizes are provided. For example, but not limited to, 3 different sizes of receiving members are provided, specifically including a first receiving member, a second receiving member, and a third receiving member. For example, but not limited to, the first container has the smallest size, the second container has the next smallest size, and the third container has the largest size, so as to satisfy the requirements for sequencing different amounts of sample to be measured; so that even small amounts of sample liquid to be tested can be sequenced. It should be understood that the first through assemblies with different sizes are provided for corresponding cooperation with the first accommodating part, the second accommodating part and the third accommodating part respectively; specifically, the device comprises a first through hole 2151 matched with the size of the opening end of the first accommodating part, a second through hole 2152 matched with the size of the opening end of the second accommodating part, and a third through hole 2153 matched with the size of the opening end of the third accommodating part.

Specifically, in order to save the usage space of the first console 212 to the maximum extent, the first through holes 2151, the second through holes 2152, and the third through holes 2153 are arranged alternately according to the characteristics such as the size and shape of the first through holes 2151, the second through holes 2152, and the third through holes 2153.

For example, but not limited to, the first container is a test tube, and/or the second container is a test tube, and/or the third container is a test tube.

Optionally, the first through hole 2151 is a circular through hole; and/or the presence of a gas in the gas,

the second through hole 2152 is a strip-shaped through hole; and/or the presence of a gas in the gas,

the third through hole 2153 is an L-shaped through hole.

Optionally, the second through hole assembly 214 includes a plurality of fourth through holes 2141 arranged at intervals and a plurality of fifth through holes 2142 arranged at intervals, and the fourth through holes 2141 and the fifth through holes 2142 have different sizes;

the second accommodating assembly 223 includes a plurality of fourth accommodating parts and a plurality of fifth accommodating parts, the fourth accommodating parts and the fourth through holes 2141 are arranged in a one-to-one correspondence manner, and the fourth accommodating parts are adapted to the fourth through holes 2141; the fifth accommodating parts and the fifth through holes 2142 are arranged in a one-to-one correspondence manner, and the fifth accommodating parts are matched with the fifth through holes 2142.

In this embodiment, in order to simultaneously sequence different amounts of sample liquids to be tested, the second accommodating components 223 with different sizes are provided. For example, but not limited to, two different sizes of receiving members are provided, specifically including a fourth receiving member and a fifth receiving member. For example, but not limited to, the fourth container is smaller than the fifth container, so that the sequencing of the refrigerated sample solution to be tested with small amount can be realized. It should be understood that different sizes of the second through-hole assembly 214 are provided for corresponding cooperation with the fourth receiving member and the fifth receiving member, respectively; specifically, the fourth through hole 2141 is matched with the size of the opening end of the fourth accommodating part, and the fifth through hole 2142 is matched with the size of the opening end of the fifth accommodating part.

For example, but not limiting of, the fourth container is a test tube, and/or the fifth container is a test tube.

Optionally, the fourth through holes 2141 are distributed at two ends of the fifth through holes 2142.

In this embodiment, the fourth through holes 2141 and the fifth through holes 2142 are disposed at intervals around the periphery of the second stage 221. For example, but not limited to, the fifth through holes 2142 are disposed at two ends of the fourth through holes 2141. It should be understood that, in other embodiments, the plurality of fourth through holes 2141 and the plurality of fifth through holes 2142 may be alternately arranged.

Optionally, the first console 212 is provided with an installation hole 211 penetrating through the first console 212, and the installation hole 211 is disposed near the center of the first console 212;

the first cartridge body 210 is mounted to a capturing and warehousing device through the mounting hole 211.

In this embodiment, for a simplified structure, a mounting hole 211 is provided on the first console 212, and the first console 212 and the capturing and warehousing device are connected and fastened through the mounting hole 211. It should be understood that the capturing and warehousing device is provided with a mounting part matched with the mounting hole 211, and a locking part for locking the mounting part and the first operating platform 212. For example, but not limiting of, the mounting member is a mounting block and the locking member is a screw/bolt.

Optionally, the first station 212 is a carousel station; and/or the presence of a gas in the gas,

the sinking groove structure 213 is a semicircular sinking groove, and the second operating platform 221 is a semicircular operating platform.

In this embodiment, in order to improve the utilization rate of the first operating platform 212, the first operating platform 212 is configured as a disk operating platform. Therefore, the through hole assembly can be arranged on the whole operation table top (except the central position) to realize multiple sequencing experiments of the sample liquid to be tested. It should be understood that the central position of the first operating platform 212 is fixedly connected to the capturing and warehousing device, and the peripheral sides of the central position are provided with operating ports (i.e. through holes or accommodating cavities for accommodating sample liquids to be measured) for capturing and warehousing experiments.

Optionally, one end of the rotating assembly 310 away from the mounting hole 211 is provided with a movable notch, the movable notch extends in the same direction as the first middle through hole, the pipetting mechanism 300 further includes a lifting assembly 330 having a second middle through hole, the second middle through hole extends in the same direction as the first middle through hole, one end of the lifting assembly 330 is rotatably connected to the rotating assembly 310 and located in the first middle through hole, and the other end of the lifting assembly 330 passes through the movable notch to expose the rotating assembly 310 and is suspended above the first reagent kit body 210 or the second reagent kit body 220;

one end of the pipetting assembly 320 is positioned in the second through hole and is communicated with a pipettor, and the other end of the pipetting assembly 320 is connected to one end of the lifting assembly 330 close to the movable notch;

the driving assembly drives the lifting assembly 330 to rotate, so as to drive the pipetting assembly 320 to extend into or withdraw from the first cartridge body 210 or the second cartridge body 220.

In this embodiment, in order to achieve the reciprocating movement of the pipetting assembly 320 in the direction toward the reagent cartridge 200 or in the direction away from the reagent cartridge 200, a lifting assembly 330 is provided. The pipetting assembly 320 is connected to the lifting assembly 330, so that the lifting assembly 330 can move up and down to drive the pipetting assembly 320 to move towards or away from the reagent kit 200 for reciprocating motion. Specifically, the lifting movement of the lifting assembly 330 is achieved by two steps of rotation and stopping. A movable gap is formed at one end of the rotating assembly 310 far away from the reagent cartridge 200, one end of the lifting assembly 330 is rotatably connected to the rotating assembly 310, and the other end of the lifting assembly 330 passes through the movable gap to be exposed out of the rotating assembly 310 and is suspended above the first reagent cartridge body 210 or the second reagent cartridge body 220; thus, when the lifting assembly 330 is rotated, the lifting assembly 330 can reciprocate in a direction approaching or separating from the reagent cartridge 200 under the limit of the movable notch by the lifting assembly 330, and further drives the liquid-transferring assembly 320 to extend into or draw out the first reagent cartridge body 210 or the second reagent cartridge body 220 to realize operations such as adding, sucking, transferring and the like of the sample liquid.

Optionally, the lifting assembly 330 includes a connecting member 331, a threaded member 332 and a lifting member 333, one end of the connecting member 331 is rotatably connected to the rotating assembly 310, the other end of the connecting member 331 is connected to one end of the threaded member 332, and both the connecting member 331 and the threaded member 332 are located in the first through hole; one end of the lifting piece 333 is rotatably connected to the threaded piece 332, and the other end of the lifting piece 333 is arranged in a suspended manner after penetrating through the movable gap;

the connecting piece 331 and the threaded piece 332 are both provided with through holes, and the through hole of the connecting piece 331 is communicated with the through hole of the threaded piece 332 to form the second middle through hole;

the driving assembly drives the screw 332 to rotate, and the lifting piece 333 abuts against the movable notch to drive the lifting piece 333 to reciprocate along the screw 332.

In this embodiment, a connecting member 331, a threaded member 332 and a lifting member 333 are provided to cooperate with each other to achieve the lifting function of the lifting assembly 330.

Specifically, the screw 332 is disposed corresponding to the movable gap, one end of the lifting member 333 is connected to the screw 332 through a screw, and the other end of the lifting member 333 is suspended through the movable gap; thus, when the screw 332 is rotated, the lifting member 333 can reciprocate toward or away from the reagent cartridge 200 under the limit of the movable notch and the screw connection with the screw 332. For example, but not limited to, the screw 332 rotates clockwise, the lifting member 333 abuts against one side of the movable notch, and the lifting member 333 moves toward the direction close to the reagent cartridge 200 with the rotation of the screw 332, that is, the lifting member 333 moves downward; the screw 332 rotates counterclockwise, the lifting member 333 abuts against the other side of the movable notch, and the lifting member 333 moves away from the reagent cartridge 200 with the rotation of the screw 332, that is, the lifting member 333 moves upward. In this manner, in the movement of "raising" or "lowering" of the lift 333, the pipetting unit 320 also "raises" or "lowers" in accordance with the movement, and operations such as addition, suction, and transfer of the sample liquid in the reagent cartridge 200 by the pipetting unit 320 are realized. Thus, the liquid-transferring mechanism 300 realizes intelligent operation of capturing and building the library of the target gene, and greatly improves the efficiency of capturing and building the library.

In one embodiment, the threaded member 332 is a threaded rod.

Specifically, one end of the connecting member 331 communicates with the threaded member 332, and the other end of the connecting member 331 is rotatably connected to the rotating component 310, so as to achieve a relative rotatable arrangement between the connecting member 331 and the rotating component 310, thereby achieving the rotation of the threaded member 332.

For a simplified structure, a first central through hole is provided on the rotating member 311, a second central through hole is provided on the lifting assembly 330, the lifting assembly 330 is disposed in the first central through hole, and the liquid-transferring assembly 320 is disposed in the second central through hole; therefore, the occupied area is reduced, the miniaturization of equipment is facilitated, the attractiveness of the liquid transfer mechanism 300 is improved, and the use experience of a user is facilitated.

Optionally, the lifting assembly 330 further includes a first abutting piece 334, one end of the first abutting piece 334 is sleeved on one end of the connecting piece 331, and the other end of the first abutting piece 334 is sleeved on one end of the screw 332; the first abutment member 334 protrudes from the connecting member 331 and the screw member 332.

In this embodiment, the radial dimension of the first abutment member 334 is greater than the radial dimensions of the connecting member 331 and the screw member 332; thus, when the lifting assembly 330 is connected to the rotating assembly 310, the outer sidewall of the first abutting member 334 abuts against the inner sidewall of the rotating member 311.

For example, but not limited to, the connecting member 331 is a connecting tube, and the first abutting member 334 is a connecting circular truncated cone. The connecting pipe is communicated with the threaded part 332, and the connecting circular truncated cone is sleeved at the connecting part of the connecting pipe and the threaded part 332 so as to enhance the connecting tightness of the connecting pipe and the threaded part 332.

Optionally, a first sealing groove is concavely formed on the first abutting piece 334, the first sealing groove extends around the circumferential direction of the sidewall of the first abutting piece 334 and is closed end to end, the lifting assembly 330 further includes a first sealing element 335 adapted to the first sealing groove, and the first sealing element 335 is embedded in the first sealing groove.

In this embodiment, a first seal 335 is provided to improve sealing. For example, but not limiting of, the first seal 335 is a gasket. The opening of the first seal groove opens toward the inner sidewall of the rotating element 310 and extends from the outer sidewall of the first abutting member 334 toward the direction away from the inner sidewall of the rotating element 310. It should be appreciated that when the outer sidewall of the first abutment 334 abuts the inner sidewall of the rotating assembly 310, the side of the first seal 335 near the open end of the first seal groove abuts the inner sidewall of the rotating assembly 310.

Optionally, both ends of the lifting member 333 are respectively provided with a rotating hole and a fixing hole penetrating through the lifting member 333; the screw 332 passes through the rotating hole and is rotatably connected to the lifting member 333, and the pipetting assembly 320 is inserted through and fixedly connected to the fixing hole of the lifting member 333.

In this embodiment, for a simplified structure, a rotation hole and a fixing hole are provided on the lifting member 333, so as to realize the rotatable connection between the lifting member 333 and the screw 332 and the fixed connection between the lifting member 333 and the pipetting assembly 320. Therefore, the three parts can be connected without other structures, and the space of the device is saved.

Optionally, the pipetting assembly 320 includes a pipette 321 and a TIP 322 which are communicated with each other, the pipette 321 passes through the second through hole to be connected to the pipette, and one end of the TIP 322 passes through and is fixedly connected to the fixing hole of the lifting member 333.

In this embodiment, for example, but not limited to, the pipette 321 is a flexible tube, and the flexible tube is accommodated in the second through hole; so, avoid the hose to expose on the one hand and cause whole device's unsightly, on the other hand accomodates the hose in lifting unit 330, separate it with external environment, avoid it to expose with aerobic air in, be favorable to reducing the ageing reaction of hose, improve the life of hose. Meanwhile, one end of the hose is communicated with the pipette, and the other end of the hose is communicated with the TIP head 322, so that the TIP head 322 can suck or discharge the sample liquid by opening or closing the pipette. It should be understood that, in order to facilitate the sucking of the sample liquid, the TIP of the TIP 322 far away from the pipette 321 is shaped like a needle, and the TIP of the TIP 322 near the pipette 321 is shaped to fit the size of the pipette 321, so as to facilitate the pipette 321 to be nested in the TIP 322.

Optionally, the rotating assembly 310 comprises a rotating member 311 and a second abutment 312;

the second abutting piece 312 is disposed around the outer wall of the rotating piece 311, and the rotating piece 311 is divided into a mounting portion and a rotating portion by the second abutting piece 312; the mounting part penetrates into the mounting hole 211 to mount the rotary member 311 to the reagent cartridge 200;

the first middle through hole penetrates through the rotating part 311, and the movable notch is arranged on the rotating part.

In this embodiment, for example, but not limited to, the rotating member 311 is a cylindrical structure; the second abutting piece 312 is a disc structure provided with a mounting through hole, and the second abutting piece 312 is sleeved outside the rotating piece 311 and divides the rotating piece 311 into a long rotating part (long) and a short mounting part (short). When the rotating unit 310 is attached to the reagent cartridge 200, the attachment portion is rotatably attached to the reagent cartridge 200, the second abutment member 312 abuts against the reagent cartridge 200, and the rotating portion is located above the reagent cartridge 200.

It should be understood that the movable notch is disposed at one end of the rotating portion far from the mounting portion, and the movable notch is opened from one end of the rotating member 311 along an axial direction of the rotating portion and extends toward a direction close to the mounting portion.

Optionally, a second sealing groove is annularly disposed on one side of the second abutting part 312 facing the mounting portion, the rotating assembly 310 further includes a second sealing element 313 adapted to the second sealing groove, and the second sealing element 313 is embedded in the second sealing groove.

In this embodiment, in order to enhance the sealing property at the connection between the second contact member 312 and the reagent cartridge 200, a second sealing groove is provided on the side where the second contact member 312 contacts the reagent cartridge 200. It should be understood that the second seal groove extends in a direction toward the rotary portion. For example, but not limiting of, the second seal groove is a seal groove ring. For example, but not limiting of, the second seal 313 is a gasket.

Optionally, the rotating assembly 310 further includes a first fixing member 314, the first fixing member 314 is sleeved on an outer side wall of the mounting portion, and the first fixing member 314 is used for fastening the rotating member 311;

the first securing member 314 and the second abutment member 312 are located on opposite sides of the cartridge 200.

In this embodiment, in order to enhance the fastening of the relative position between the rotating assembly 310 and the reagent cartridge 200, a first fixing member 314 is provided. For example, but not limited to, the first fixing element 314 is a circular truncated cone structure provided with a through fixing hole. When the mounting portion is mounted in the mounting hole 211, the second abutting member 312 and the rotating member 311 are located above the reagent cartridge 200, the first fixing member 314 is sleeved outside the mounting portion, and one side of the first fixing member 314 abuts against the reagent cartridge 200 and is located below the reagent cartridge 200; in this way, a sandwich structure of the second contact member 312, the reagent cartridge 200, and the first fixing member 314 is formed on both sides of the reagent cartridge 200, so as to prevent the rotation member 311 from swinging left and right during rotation, thereby causing an unnecessary influence on the stability of the liquid transfer operation.

Optionally, an abutting boss is convexly disposed on an inner wall of one end of the mounting portion, which is close to the rotating portion, the lifting assembly 330 further includes a second fixing member 336, the second fixing member 336 is sleeved on an outer side wall of the connecting member 331, and the second fixing member 336 is used for fastening the connecting member 331;

the second fixing member 336 and the first abutting member 334 abut against opposite sides of the abutting boss.

In this embodiment, in order to enhance the fastening of the relative positions of the lifting assembly 330 and the rotating assembly 310, a second fixing member 336 is provided. For example, but not limited to, the second fixing member 336 is a circular truncated cone structure with a through fixing hole. When the lifting assembly 330 is rotatably mounted in the first through hole of the rotating assembly 310, one end of the connecting member 331 abuts against one side of the abutment boss, and the other end of the connecting member 331 passes through a small through hole defined by the abutment boss; the second fixing member 336 is sleeved outside the other end of the connecting member 331, and one end of the second fixing member 336 abuts against the other side of the abutting boss; thus, a sandwich structure is formed on two sides of the abutting boss, so that the lifting assembly 330 is prevented from swinging left and right in the rotating process, and unnecessary influence is caused on the stability of the pipetting operation.

Optionally, the driving assembly includes a first driving member (not shown) and a second driving member (not shown) disposed on the device body 100, the first driving member is used for driving the rotating assembly 310 to rotate 360 ° around the mounting hole 211, and the second driving member is used for driving the screw 332 to rotate.

In this embodiment, for example and without limitation, the first driving member is a driving motor, and the second driving member is a driving motor. The first and second drivers are both mounted within the device body.

Optionally, the capturing and library building device further comprises a casing 400 with an open end, wherein the open end of the casing 400 is detachably sleeved on the reagent kit 200, so as to form a closed space for sequencing and library building operation between the casing 400 and the reagent kit 200;

the housing 400 is mounted at a mounting position of the apparatus body 100; the pipetting mechanism 300 is located in the enclosed space.

In this embodiment, in order to reduce the interference of the external environment on the sequencing library building operation, the housing 400 is provided. For example, but not limiting of, the housing 400 is barrel-shaped.

Specifically, the casing 400 has an open end, and the reagent cartridge 200 has an abutting side wall, which abuts against an inner wall of the casing 400 when the reagent cartridge 200 is fitted to the open end of the casing 400, and forms a closed space for sequencing library construction between the casing 400 and the reagent cartridge 200. Therefore, on one hand, the kit 200 is used as a part of a closed space enclosing to form sequencing and library building operation, so that the use of raw materials is reduced, and the cost is reduced; on the other hand, by constructing a closed operation space, the interference of an external environment on the sequencing library building operation is avoided, and the library building success rate is improved.

Specifically, in order to fasten the housing 400 and the reagent cartridge 200, a fastening hole 401 is formed in the housing 400, and a locking protrusion 230 is formed on a peripheral side wall (i.e., an abutting side wall) of the reagent cartridge 200. In this way, the fastening of the reagent kit 200 to the housing 400 is achieved by the snap connection between the locking protrusion 230 and the locking hole 401. It should be understood that the housing 400 is removably attachable to the cartridge 200. In this way, when the parts inside the housing 400 need to be overhauled, the restriction between the locking protrusion 230 and the locking hole 401 can be removed, the whole operation is convenient and easy, and the practicability of the capturing and warehouse building device is improved.

Optionally, the snap projection 230 has an abutting surface 231, and the abutting surface 231 is disposed on a side of the snap projection 230 facing the housing 400; the abutting surface 231 comprises a connected inclined segment 2311 and a parallel segment 2312, the parallel segment 2312 is connected with the reagent kit 200, and one end of the inclined segment 2311 far away from the parallel segment 2312 is arranged in the air.

In this embodiment, in order to reduce the force for assembling the casing 400 and the reagent cartridge 200 and improve the acceptance of the assembling operator, the abutting surface 231 of the locking protrusion 230 is provided with an inclined section 2311 and a parallel section 2312. In this way, when the locking protrusion 230 is fitted into the locking hole 401, the biasing force of the assembling worker can be effectively reduced by the component force of the inclined section 2311, which is advantageous for the assembling work.

Optionally, the tight lock hole 401 is provided with a plurality of tight lock holes 401, a plurality of tight lock holes 401 are arranged around the peripheral side wall of the casing 400 at intervals, the clamping protrusion 230 is provided with a plurality of clamping protrusions 230, a plurality of clamping protrusions 230 are arranged around the peripheral side wall of the reagent kit 200 at intervals, and the clamping protrusions 230 and the tight lock holes 401 are arranged in a one-to-one correspondence manner.

In this embodiment, in order to improve the fastening of the case 400 and the reagent cartridge 200, a plurality of locking holes 401 and a plurality of locking protrusions 230 are provided. It should be understood that each of the locking protrusions 230 is correspondingly engaged with one of the locking holes 401 to complete the snap connection.

Alternatively, a plurality of the locking holes 401 are located on the same horizontal plane, and a plurality of the locking protrusions 230 are located on the same horizontal plane.

In this embodiment, in order to improve the processing efficiency, the fastening holes 401 are disposed on the same circumference, and the locking protrusions 230 are disposed on the same circumference. It should be understood that each of the locking protrusions 230 is correspondingly engaged with one of the locking holes 401 to complete the snap connection.

Alternatively, a plurality of the locking holes 401 are located on different horizontal planes, and a plurality of the locking protrusions 230 are located on different horizontal planes.

In this embodiment, in order to enhance the connection tightness between the plurality of locking protrusions 230 and the plurality of locking holes 401 and to disperse the force-bearing area of the housing 400, the plurality of locking holes 401 are disposed on different circumferences, and the plurality of locking protrusions 230 are disposed on different circumferences. Therefore, the fastening holes 401 are dispersedly formed in the shell 400, and the clamping protrusions 230 are dispersedly formed in the peripheral side wall of the reagent kit 200, so that the stress points of the shell 400 are effectively dispersed, the limitation of the clamping protrusions 230 on the shell 400 is effectively improved, and the connection tightness between the shell 400 and the reagent kit 200 is effectively improved.

Optionally, the housing 400 is a transparent housing.

In this embodiment, in order to observe the sequencing library building operation, the housing 400 is set to be a transparent housing. Thus, the internal operation of the capture library building device can be directly observed and recorded.

Optionally, the material of the housing 400 is one or a combination of polymethyl methacrylate, polystyrene, polycarbonate, styrene acrylonitrile, or styrene-methyl methacrylate copolymer.

In this embodiment, for example, but not limited to, the material of the housing 400 is polymethyl methacrylate (PMMP).

Optionally, a limiting structure 410 is further disposed on the casing 400, and the limiting structure 410 is convexly disposed on the inner wall of the casing 400 near the open end.

In this embodiment, in order to limit the position of the reagent kit 200 extending into the casing 400, a limiting structure 410 is provided. When the reagent kit 200 is inserted into the housing 400, one end of the reagent kit 200 abuts against the limiting structure 410, and the locking protrusion 230 convexly arranged on the reagent kit 200 is embedded into the locking hole 401 arranged on the housing 400, so that the assembly of the housing 400 and the reagent kit 200 is completed.

Optionally, the limiting structure 410 is a limiting convex ring.

In this embodiment, in order to facilitate the processing, the limiting structure 410 is configured as a limiting convex ring.

It should be understood that in other embodiments, the limiting structure 410 may also be a plurality of limiting bosses which are spaced around the inner side wall of the circumference of the shell 400 and form a circular shape in a surrounding manner.

Optionally, in order to improve the sealing performance at the joint between the casing 400 and the reagent cartridge 200, a sealing member 420 is protruded on the peripheral sidewall of the reagent cartridge 200. For example, but not limiting of, the seal 420 is a soft rubber seal. It should be understood that the sealing member 420 is disposed apart from the chucking protrusion 230 in the axial direction of the cartridge 200.

In one embodiment, the sealing ring is positioned above the snap boss 230 to be disposed closer to the housing 400.

Optionally, in order to facilitate the addition of the sample liquid, a sample hole 402 and a sample hole 403 are formed in a side of the housing 400 away from the kit 200, so as to facilitate the addition of the sample liquid to be stored and the extraction of the sample liquid after the storage is completed. It should be understood that the sample application aperture 402 and the sample aperture 403 are disposed on opposite sides.

Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.

Claims (10)

1. A capture library building apparatus, comprising:

the device body is provided with a mounting position;

the kit is arranged at the installation position; the kit is used for capturing the target gene of library construction; the kit comprises a first kit body and a second kit body detachably connected to the first kit body, wherein the first kit body is provided with a mounting hole; the first kit body is used for loading a normal-temperature sequencing sample liquid, and the second kit body is used for loading a sequencing sample liquid to be refrigerated;

the liquid transferring mechanism comprises a rotating assembly, a liquid transferring assembly and a driving assembly; the rotating assembly is rotatably arranged in the mounting hole and is provided with a first through hole penetrating through the rotating assembly, and the first through hole extends along the axial direction of the rotating assembly; one end of the liquid transfer assembly is positioned in the first middle through hole and is communicated with the liquid transfer device, and the other end of the liquid transfer assembly is exposed out of the first middle through hole and is suspended in the first kit body or the second kit body; the driving assembly is arranged on the device body and used for driving the rotating assembly to rotate around the mounting hole by 360 degrees, and the driving assembly is used for driving the pipetting assembly to stretch into or draw out the first kit body or the second kit body.

2. A capturing and warehousing device according to claim 1, characterized in that the first kit body comprises a first operating platform and a sinking groove structure, the sinking groove structure is concavely arranged on the first operating platform, and a second through hole component penetrating through the first operating platform is arranged in the sinking groove structure;

the second kit body comprises a second operation table matched with the sinking groove structure, and the second operation table is detachably connected with the sinking groove structure;

the mounting hole is formed in the first operating platform.

3. A capturing and library building device according to claim 2, wherein the first operation platform is provided with a first through hole assembly penetrating through the first operation platform, the first through hole assembly and the second through hole assembly are arranged at intervals, the first kit body further comprises a first accommodating assembly, the first accommodating assembly is provided with an open end and a first accommodating cavity, the open end of the first accommodating assembly is connected to the first operation platform, and the open end of the first accommodating assembly is communicated with the first through hole assembly to form a channel for inserting or extracting the first accommodating cavity;

the second operation platform is provided with a third through hole assembly which penetrates through the second operation platform, the third through hole assembly is matched with the second through hole assembly, the second kit body further comprises a second containing assembly, the second containing assembly is provided with an opening end and a second containing cavity, the opening end of the second containing assembly is connected to the second operation platform, and the opening end of the second containing assembly is communicated with the third through hole assembly to form a channel for inserting or extracting the second containing cavity;

the driving assembly drives the liquid-transfering assembly to stretch into or draw out the first accommodating cavity or the second accommodating cavity.

4. A capture library building apparatus according to claim 3, wherein the first via assembly comprises a plurality of first vias, a plurality of second vias and a plurality of third vias, the first vias, the second vias and the third vias being of different sizes;

the first through holes, the second through holes and the third through holes are arranged on the first operating platform in a staggered mode at intervals;

the first accommodating assembly comprises a plurality of first accommodating pieces, a plurality of second accommodating pieces and a plurality of third accommodating pieces, the plurality of first accommodating pieces and the plurality of first through holes are arranged in a one-to-one correspondence manner, and the first accommodating pieces are matched with the first through holes; the plurality of second accommodating pieces and the plurality of second through holes are arranged in a one-to-one correspondence manner, and the second accommodating pieces are matched with the second through holes; the plurality of third accommodating pieces and the plurality of third through holes are arranged in a one-to-one correspondence manner, and the third accommodating pieces are matched with the third through holes.

5. The capture library building device of claim 4, wherein the second via assembly comprises a plurality of spaced fourth vias and a plurality of spaced fifth vias, the fourth vias and the fifth vias being different sizes;

the second accommodating assembly comprises a plurality of fourth accommodating pieces and a plurality of fifth accommodating pieces, the fourth accommodating pieces and the fourth through holes are arranged in a one-to-one correspondence manner, and the fourth accommodating pieces are matched with the fourth through holes; the plurality of fifth accommodating parts and the plurality of fifth through holes are arranged in a one-to-one correspondence manner, and the fifth accommodating parts are matched with the fifth through holes.

6. The capturing and warehousing device of claim 5, wherein an end of the rotating assembly away from the installation hole is provided with a movable notch, the movable notch extends in the same direction as the first middle through hole, the pipetting mechanism further comprises a lifting assembly having a second middle through hole, the second middle through hole extends in the same direction as the first middle through hole, one end of the lifting assembly is rotatably connected to the rotating assembly and located in the first middle through hole, and the other end of the lifting assembly passes through the movable notch to expose the rotating assembly and is suspended above the first kit body or the second kit body;

one end of the liquid transferring assembly is positioned in the second middle through hole and is communicated with the liquid transferring device, and the other end of the liquid transferring assembly is connected to one end, close to the movable notch, of the lifting assembly;

the driving component drives the lifting component to rotate so as to drive the pipetting component to stretch into or draw out the first kit body or the second kit body.

7. A capturing and warehousing device as claimed in claim 6, characterized in that the lifting assembly comprises a connecting member, a screw member and a lifting member, one end of the connecting member is rotatably connected to the rotating assembly, the other end of the connecting member is connected to one end of the screw member, and the connecting member and the screw member are both located in the first through hole; one end of the lifting piece is rotatably connected to the threaded piece, and the other end of the lifting piece penetrates through the movable notch and is arranged in a suspended mode;

the connecting piece and the threaded piece are both provided with through holes, and the through holes of the connecting piece and the through holes of the threaded piece are communicated to form second middle through holes;

the driving assembly drives the threaded piece to rotate, and the lifting piece abuts against the movable notch so as to drive the lifting piece to reciprocate along the threaded piece.

8. The capture library building device of claim 7, wherein the pipetting assembly comprises a pipette and a TIP head in communication, the pipette is connected to the pipette through the second through hole, and one end of the TIP head is inserted through and fixedly connected to the fixing hole of the lifting member.

9. The apparatus for capturing and building a warehouse as claimed in claim 8, wherein the driving assembly comprises a first driving member and a second driving member, the first driving member is disposed on the apparatus body and is used for driving the rotating assembly to rotate 360 ° around the mounting hole, and the second driving member is used for driving the screw member to rotate.

10. The capture library-building device of any one of claims 1 to 9, further comprising a housing having an open end, wherein the open end of the housing is detachably sleeved on the reagent kit to form a closed space for sequencing library-building operation between the housing and the reagent kit;

the shell is arranged at the installation position of the device body; the pipetting mechanism is located in the enclosed space.

Images