CN219807940U - Sequencing library prepares cartridge and preparation appearance thereof - Google Patents

Abstract

The utility model provides a sequencing library preparation card box and a preparation instrument thereof, relating to the technical field of gene sequencing, comprising a shell, wherein the top of the shell is provided with an opening, a kit is arranged in the shell and is in airtight connection with the shell, and the kit is provided with a plurality of object placing cavities; the movable assembly is provided with a gun head and an interface, the interface is used for being connected with an external driving mechanism, the movable assembly is rotatably connected in the shell and can move along the axial direction of the shell so that the gun head is aligned with and inserted into the storage cavity alternatively, and a closed cavity is formed between the movable assembly and the reagent box. The problem that the cartridge in the prior art cannot simply realize closed sequencing is solved.

Description

Sequencing library prepares cartridge and preparation appearance thereof

Technical Field

The utility model relates to the technical field of gene sequencing, in particular to a sequencing library preparation cartridge and a preparation instrument thereof.

Background

After the 50 s of the 20 th century, with the development of molecular genetics, it was gradually recognized that the nature of genes is DNA fragments that control biological traits and have genetic effects. On one hand, the gene can faithfully perform self-replication so as to ensure the basic characteristics of organisms; on the other hand, the gene can also be mutated, resulting in the occurrence of diseases. The genetic sequencing can rapidly and accurately acquire biological genetic information, reveal the complexity and diversity of genome, and play a very important role in life science research; likewise, gene sequencing is of great significance and value to clinical medicine, and the mechanism research and clinical diagnosis of many diseases are increasingly dependent on gene sequencing technology.

Currently, the traditional main mode of preparing the gene sequencing library is finished by manual operation, and the processes of sample adding, temperature bath reaction, purification, elution and the like are all finished by human participation, and meanwhile, the whole process is finished in a relatively open laboratory environment. The disadvantage of this operation is that the environment is very prone to contamination of the sample solution.

Disclosure of Invention

In view of the shortcomings of the prior art, a first object of the present utility model is to provide a sequencing library preparation cartridge, which solves the problem that the cartridge in the prior art cannot simply realize closed sequencing.

According to an embodiment of the utility model, a sequencing library preparation cartridge comprises:

a shell, the top of which is provided with an opening for the driving mechanism to penetrate,

the kit is arranged in the shell and is in airtight connection with the shell, and the kit is provided with a plurality of storage cavities;

the moving assembly is provided with a gun head and an interface, the interface is used for being connected with an external driving mechanism, the moving assembly is rotatably connected in the shell and can move along the axial direction of the shell, so that the gun head is aligned alternatively and inserted into the storage cavity, and a closed cavity is formed between the moving assembly and the reagent box.

Compared with the prior art, the utility model has the following beneficial effects:

1. the kit is connected with the shell in a sealing way, a sealing cavity is formed between the moving component and the kit, so that the shell, the kit and the moving component form an integral sealing space when in operation; when the automatic sequencing device is used, an external driving mechanism stretches into the opening and is connected with the interface, the driving mechanism can drive the moving assembly to rotate relative to the shell as required, so that the gun head is rotated to align with different storage cavities, the driving mechanism can drive the moving assembly to move up and down along the axis of the shell, so that the gun head is pulled out or inserted into the storage cavities, the gun head is externally connected with a pipetting mechanism, sampling liquid is sucked or discharged, the sample liquid is moved in different storage cavities, and the whole sequencing process is in a sealed clamping box, so that the influence of the environment on the sample liquid is greatly reduced.

2. On one hand, only the moving component moves up and down or rotates circumferentially in the working process of the cartridge, so that the arrangement and the installation of the driving mechanism are facilitated, and the cartridge is simple and convenient to position and install due to the fact that the cartridge does not need to move; on the other hand, the cartridge of the utility model does not need to integrate a driving mechanism as a consumable, thereby greatly simplifying the structure of the cartridge and reducing the cost of the consumable.

Further, the shell is cylindrical, the outer wall of the kit is attached to the inner wall of the shell, and the kit is provided with a limiting groove which is recessed downwards;

the moving assembly includes:

one end of the rod part is inserted into the limit groove and can rotate relatively;

and the disc is fixedly arranged on the rod part, and the outer wall of the disc is attached to the inner wall of the shell.

Further, the annular side of the disc is bent downwards and is attached to the inner wall of the shell.

Further, the rod portion is hollow, the interface is in a notch shape, and the interface is recessed downwards on the inner wall of the rod portion.

Further, through holes corresponding to the object placing cavities one by one are formed in the moving assembly, and the through holes and the object placing cavities are sealed through hot melt films.

Further, the shell and the moving assembly are provided with a positioning module for positioning the moving assembly.

Further, the top of shell is along the turn-ups of its centre of a circle buckling, positioning module includes the lug, and it is equipped with a plurality of and distributes the bottom of turn-ups, but moving assembly is last to have set firmly vertical joint to the draw-in groove of lug, the bottom of shell is equipped with the opening.

Further, an elastic component is arranged between the connecting component and the kit and is used for driving the connecting component to move upwards.

In view of the shortcomings of the prior art, a second object of the present utility model is to provide a sequencing library preparation apparatus, which solves the problem that the preparation apparatus in the prior art cannot simply realize closed sequencing.

A sequencing library preparation instrument for use in a sequencing library preparation cartridge as described above, comprising:

the frame is provided with a lifting mechanism and a rotating mechanism, the lifting mechanism can drive the rotating mechanism to move up and down, the rotating mechanism is provided with a connecting component, the free end of the connecting component is connected with the interface to drive the moving component to rotate, and the connecting component is provided with a pushing part which can be abutted against the end face of the moving component;

and the pipetting mechanism is vertically and movably connected to the frame through the lifting mechanism and is communicated with the gun head for sucking or extruding sample liquid.

Compared with the prior art, the utility model has the following beneficial effects:

when the gun head is used, the lifting mechanism drives the rotary mechanism to move downwards until the free end of the connecting component passes through the opening of the shell and is connected with the interface, and at the moment, the lifting mechanism continuously drives the rotary mechanism to drive the connecting component to move downwards together so as to insert the gun head into a required storage cavity, and the liquid transferring mechanism sucks sample liquid or discharges the sample liquid into the storage cavity; the lifting mechanism drives the moving assembly to move upwards or downwards, and the rotating mechanism drives the moving assembly to rotate circumferentially, so that the sample liquid can move in different storage cavities, the whole sequencing process is carried out in a sealed cartridge, and the influence of the environment on the sample liquid is greatly reduced.

The slewing mechanism and the lifting mechanism are integrated on the preparation instrument, so that the use cost of the preparation card box serving as consumable materials is reduced; in the use process, only the rotating mechanism and the lifting mechanism are needed to drive the moving assembly to lift and rotate as required, and the structure of the preparation card box and the preparation instrument is more compact, simpler and more convenient to connect.

Further, a pressing mechanism for pressing the card box is also arranged on the frame.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a cartridge according to an embodiment of the present utility model;

FIG. 2 is a schematic view of the internal structure of FIG. 1;

FIG. 3 is a schematic diagram of the explosive structure of FIG. 1;

FIG. 4 is a schematic view of an assembly of a cartridge and a connection assembly according to an embodiment of the present utility model;

FIG. 5 is a schematic view of the overall structure of the cartridge, the swing mechanism, the lifting mechanism and the pipetting mechanism in the embodiment of the utility model;

fig. 6 is a schematic structural diagram of a preparation apparatus according to an embodiment of the present utility model.

In the above figures:

1. a housing; 2. a kit; 3. a moving assembly; 4. a lifting mechanism; 5. a slewing mechanism; 6. a pipetting mechanism; 7. a compressing mechanism; 8. a magnetic attraction mechanism; 9. a cooling mechanism;

101. a bump;

201. a storage cavity; 202. a clamping protrusion;

301. a gun head; 302. an interface; 303. a stem portion; 304. a disc; 305. a through hole; 306. a clamping groove;

401. a lifting plate;

501. a connection assembly; 502. a pipetting push-pull plate; 5011. a liquid suction channel; 5012. a top plate; 5013. a lower plate; 5014. a round bar 5014; 5015. positioning columns;

601. a piston rod; 602. a connector;

701. and (5) pressing plates.

Detailed Description

In order to make the objects, technical solutions and advantageous effects of the present utility model more apparent, the technical solutions of the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.

Referring to fig. 1-3, the present utility model provides a sequencing library preparation cartridge comprising:

the shell 1, the top of the shell 1 is provided with an opening for the driving mechanism to penetrate;

the kit 2, the kit 2 is installed in the outer casing 1 and the two are connected in a sealing way, in order to facilitate the assembly and disassembly of the kit 2, the kit 2 is detachably connected to the outer casing 1, specifically, the detachable connection mode can be the existing fastening connection, bolt connection, pin connection and the like, in this embodiment, the fastening protrusion 202 is fixedly arranged on the ring side of the kit 2, and the fastening protrusion 202 is fastened on the fastening hole of the outer casing 1; the kit 2 is provided with a plurality of storage cavities 201 for containing sample liquid and/or reagent, the storage cavities 201 can be shell-shaped cavities with open tops and conical bottoms, each storage cavity 201 is distributed around the axial direction of the kit 2, the specific number and the size of the storage cavities 201 are determined according to practical application requirements, and the limitation is not imposed here;

a moving assembly 3 provided with a gun head 301 and an interface 302, wherein the gun head 301 is preferably an existing TIP head, and the top of the gun head 301 is open and is arranged on the moving assembly 3; the interface 302 is used for being connected with an external driving mechanism, the moving component 3 is rotatably connected in the shell 1, and can move along the axial direction of the shell 1, so that the gun head 301 is alternatively aligned and inserted into the storage cavity 201, and a closed cavity is formed between the moving component 3 and the kit 2.

In the present embodiment of the present utility model,

1. the kit 2 is connected with the shell 1 in a sealing way, a sealing cavity is formed between the moving component 3 and the kit 2, so that the shell 1, the kit 2 and the moving component 3 form a whole sealing space in a working way (in the sequencing process of the kit, the gun head 301 is sealed through a connector 602, and each through hole 305 is covered with a film and sealed in a hot melting way, and the details are Wen Zhuishu); when the device is used, an external driving mechanism stretches into the opening and is connected with the interface 302, the driving mechanism can drive the moving assembly 3 to rotate relative to the shell 1 as required, so that the gun head 301 is rotated to be aligned with different storage cavities 201, the driving mechanism can drive the moving assembly 3 to move up and down along the axis of the shell 1, so that the gun head 301 is pulled out or inserted into the storage cavity 201, the gun head 301 is externally connected with the pipetting mechanism 6, sampling liquid is sucked or discharged, the sample liquid is moved in different storage cavities 201, and the whole sequencing process is carried out in a sealed cartridge, so that the influence of the environment on the sample liquid is greatly reduced.

2. On one hand, only the moving component 3 moves up and down or rotates circumferentially in the working process of the cartridge, which is favorable for the arrangement and installation of a driving mechanism, and the structure of the cartridge 2 and the positioning and installation of the preparation instrument are simple and convenient because the cartridge 2 does not need to move; on the other hand, the cartridge of the utility model does not need to integrate a driving mechanism as a consumable, thereby greatly simplifying the structure of the cartridge and reducing the cost of the consumable.

In another embodiment of the present utility model, the main difference between the present utility model and the above embodiment is that the structure of the moving component 3 is optimized, as shown in fig. 2 and 3, considering that in practice, additional reagents or sample solutions need to be added into the sealed storage cavity 201 (for example, when the later added reagents need to be refrigerated and are not suitable for being added into the storage cavity 201 in advance in a special storage environment), and after the addition is finished, a sealed containing cavity is defined between the moving component 3 and the reagent kit 2, and correspondingly through holes 305 corresponding to the storage cavities 201 one by one are provided on the moving component 3, and the through holes 305 need to be sealed after the reagents/sample solutions are added into the storage cavity 201; the sealing only needs to seal the opening of the through hole 305, specifically, the sealing mode includes, but is not limited to, covering a film at the opening of the through hole 305, and sealing is realized by using a hot-melt film of the existing sealing device, the opening of the storage cavity 201 can also be sealed in the above mode, and in order to seal the through hole 305 conveniently, the top end face of the moving assembly 3 is moved to the top end of the housing 1 during sealing; therefore, the applicability of the card box is enhanced, and the sealing is convenient.

Further, the opening of the storage cavity 201 and the top of the through hole 305 are both provided to be convex upwards, which is more beneficial for the film to seal the opening.

Further, each through hole 305 is marked with a serial number, so that an operator can conveniently and quickly identify the pre-added sample liquid in the storage cavity 201 which is the object of the through hole 305, and the operation efficiency and accuracy are improved.

Further, the storage cavity 201 is at least divided into a normal temperature sequencing cavity and a cold storage sequencing cavity, the normal temperature sequencing cavity is used for loading normal temperature sequencing sample liquid, and the cold storage sequencing cavity is used for loading the sequencing sample liquid to be refrigerated.

In another embodiment of the present utility model, as shown in fig. 1, a specific structure of one of the reagent kit 2 and the moving assembly 3 is given on the basis of the above embodiment, in order to simply and conveniently realize the airtight connection and assembly of the reagent kit 2, the moving assembly 3 and the housing 1, the housing 1 is configured to be cylindrical, the reagent kit 2 is configured to be cylindrical in particular,

the outer wall of the kit 2 is attached to the inner wall of the housing 1, the center of the kit 2 is provided with a downward concave limit groove, and the limit groove can be prevented from being arranged as a cylindrical surface, so that, on one hand, the rod part 303 (described later) can be ensured to rotate in the limit groove, and on the other hand, the rod part 303 can be subjected to limit guiding effect;

the moving assembly 3 includes:

the rod part 303, one end of which is inserted into the limit groove and can rotate relatively, and the mode of realizing the relative rotation of the rod part and the limit groove comprises but is not limited to clearance fit or connection through a bearing;

the disc 304 is fixedly arranged on the rod part 303, and the outer wall of the disc 304 is attached to the inner wall of the shell 1; preferably, the disc 304 and the stem 303 are integrally formed in this embodiment.

Therefore, the outer wall of the kit 2 and the inner wall of the shell 1, and the outer wall of the disc 304 and the inner wall of the shell 1 can be simply and hermetically connected, and of course, in order to improve the sealing effect, a sealing gasket or smearing sealing oil and the like can be arranged between the two in practice; the disc 304, the rod 303 and the limiting groove can limit the moving assembly 3 to swing left and right and play a guiding role, the structure of the cartridge is simple, and the moving assembly 3 runs stably.

In another embodiment of the present utility model, as shown in fig. 1 and 2, the main difference between the structure of the disc 304 and the above embodiment is that the contact surface between the outer wall of the disc 304 and the inner wall of the housing 1 can be increased to improve the motion stability and sealing effect of the moving assembly 3, specifically, the annular side of the disc 304 is set to bend downwards and is attached to the inner wall of the housing 1, so that the disc 304 is less prone to shake from side to side and has good sealing effect with the inner wall of the housing 1.

In another embodiment of the present utility model, as shown in fig. 1 and 2, considering the convenience of using the cartridge, a positioning module for positioning the moving assembly 3 is provided between the housing 1 and the moving assembly 3, and the positioning module functions to accurately position the moving assembly 3 and the housing 1 initially, so that when the cartridge is mounted on the preparation apparatus, the connecting assembly 501 can be inserted into the interface 302 by vertically moving, and rapid assembly of the connecting assembly 501 and the interface 302 is realized;

for example, but not limited to, the top of the casing 1 is bent along the center of the circle to form a flanging, the positioning module comprises a plurality of protruding blocks 101 which are distributed at the bottom of the flanging, the moving assembly 3 is fixedly provided with a clamping groove 306 which can be vertically clamped to the protruding blocks 101, and the bottom of the casing 1 is provided with an opening; in this way, the moving assembly 3 is plugged in from the bottom of the housing 1, the moving assembly 3 is turned to perform fine adjustment, the clamping groove 306 is clamped on the protruding block 101, the assembly of the moving assembly 3 and the housing 1 is completed, and the position of the moving assembly 3 relative to the housing 1 is fixed.

In another embodiment of the present utility model, an elastic component (not shown in the figure) is added between the connection component 501 and the kit 2, and the elastic component drives the connection component 501 to move upwards by elastic force so as to reset the moving component 3 upwards when external force is removed; in order to simplify the structure of the cartridge, in particular, the elastic assembly comprises a spring, which is sleeved on the rod 303, the two ends of the spring respectively abut against the opposite ends of the moving assembly 3 and the reagent cartridge 2.

The assembly method of the card box provided by the utility model comprises the following steps:

s1, adding a required reagent into the storage cavity 201 in advance and sealing, specifically, sealing by adopting hot melting equipment matched with hot melt adhesive, so that a coating film at the top of the storage cavity 201 can be directly pierced when the gun head 301 moves downwards;

s2, penetrating the moving assembly 3 from the opening at the bottom of the shell 1 and moving upwards until the clamping groove 306 is clamped on the convex block 101, and sealing the through hole 305 at the moment;

s3, penetrating the kit 2 from the opening at the bottom of the shell 1 and connecting the kit to the bottom of the shell 1.

In the present embodiment of the present utility model, in the present embodiment,

firstly, assembling the moving assembly 3, rotating the moving assembly 3 according to the requirement, and not assembling an elastic assembly at the moment, smoothly rotating a clamping groove 306 on the moving assembly 3 to align the convex block 101, so as to realize circumferential limit of the moving assembly 3, sleeving the elastic assembly on a rod 303, and finally assembling the kit 2 at the bottom of the shell 1, wherein the kit 2 is connected with the shell 1 into a whole, so that axial limit of a limit assembly is realized; the card box has simple structure, convenient assembly and firm positioning. In addition, after the assembly, staff can pass through the through-hole 305 and finally add additional sample liquid into the storage cavity 201 as required, and finally the movable assembly 3 is located at the top of the shell 1, so that the through-hole 305 on the movable assembly 3 is conveniently sealed, and a closed cavity is formed between the movable assembly 3 and the kit 2.

The utility model also provides a sequencing library preparation instrument, which is used for the sequencing library preparation cartridge, as shown in fig. 4 to 6, and comprises:

the rack is a platform constructed according to the position connection relation of each part in the embodiment, and is erected according to the need in practice, and will not be described here again;

in order to drive the moving assembly 3 to rotate, a rotating mechanism 5 is arranged on the frame, the rotating mechanism 5 can be realized by a gear motor, for example, but not limited to, the rotating mechanism 5 is provided with a connecting assembly 501, two ends of the connecting assembly 501 are respectively connected with the output end of the rotating mechanism 5 and the moving assembly 3 to transmit power to the moving assembly 3, and in addition, a pushing part which can be abutted against the end surface of the moving assembly 3 is arranged on the connecting assembly 501 and used for pushing the moving assembly 3 to move downwards; specifically, the connecting assembly 501 includes an upper disc 5012, a lower disc 5013, and a round rod 5014 connecting the upper disc 5012 and the lower disc 5013, wherein the upper disc 5012 is detachably and fixedly connected with the output end of the slewing mechanism 5 through a fastener such as a bolt or a screw, the bottom end of the round rod 5014 is provided with a thin shell-shaped positioning column 5015, the positioning column 5015 is coaxial with the round rod 5014, and the inner diameter of the positioning column 5015 is smaller than the inner diameter of the round rod 5014, so that a shaft shoulder is formed, and the shaft shoulder plays the role of the pushing part; in this way, the connecting component 501 has a simple structure, is reliable in positioning, and can be conveniently connected with and disconnected from the moving component 3;

in order to drive the slewing mechanism 5 to move up and down, and further drive the moving assembly 3 to move together, a lifting mechanism 4 is further arranged on the frame, for example, but not limited to, the lifting mechanism 4 is provided with a first linear driving assembly and a lifting plate 401;

specifically, the lifting plate 401 is slidably connected to the frame through a vertical sliding rail, the moving end of the first linear driving assembly is fixedly/detachably connected with the lifting plate 401, the first linear driving assembly can specifically select an existing screw rod, an electric push rod/oil cylinder and the like, the slewing mechanism 5 is mounted on the lifting plate 401, and the first linear driving assembly can drive the slewing mechanism 5 to move up and down by driving the lifting plate 401;

in order to facilitate positioning and installing the cartridge, the rack is further provided with an installing position, for example, but not limited to, the installing position is provided with a plurality of positioning holes with top openings, the inner diameter of each positioning hole is slightly larger than the outer diameter of the object placing cavity 201, and each positioning hole can be aligned with each object placing cavity 201 one by one, so that the object placing cavity 201 can be smoothly inserted into each positioning hole to realize positioning; in addition, a strip-shaped convex edge can be arranged at the bottom of the kit 2, a bayonet matched with the strip-shaped convex edge is arranged on the mounting position, and the strip-shaped convex edge is clamped in the bayonet to rapidly position the kit 2; the two cassettes and the rack can be used alternatively or in combination;

in order to meet the requirements of adding, sucking and transferring the sample liquid in the reagent kit 2, a pipetting mechanism 6 is arranged, the pipetting mechanism 6 is vertically and movably connected to the rack through a lifting mechanism 4 so as to keep the relative positions of the pipetting mechanism 6 and a rotating mechanism 5, and in particular, in order to simplify the structure, the pipetting mechanism 6 is also arranged on a lifting plate 401, and the pipetting mechanism 6 and the rotating mechanism 5 can be synchronously lifted or lowered by adopting a set of lifting mechanism 4;

the pipetting mechanism 6 is communicated with the gun head 301 and is used for sucking or extruding sample liquid; specifically, in order to enhance the aesthetic appearance and protect the connection assembly 501, the liquid suction channel 5011 is disposed inside the connection assembly 501, and the liquid suction channel vertically passes through the upper disc 5012 and the round bar 5014 and then extends along the radial direction of the lower disc 5013;

the pipetting mechanism 6 is provided with a piston rod 601, a second linear driving assembly, a connector 602 and a pipetting push-pull plate 502, wherein the piston rod 601 is movably connected to the top end of the pipetting channel 5011 and can move along the vertical section of the pipetting channel 5011, the top end of the connector 602 is arranged at the bottom end of the pipetting channel 5011, the other end of the connector 602 can be inserted into the interface 302 and is communicated with the interface 302, the middle part of the connector 602 is communicated, and the upper end and the lower end of the connector 602 are respectively required to be sealed with the connecting assembly 501 and the moving assembly 3, so that liquid can only pass through the middle channel of the connector 602; specifically, the connector 602 may be a rubber head, and the sealing at the connection between the top end of the connector 602 and the connecting component 501 and the sealing at the connection between the bottom end of the connector 602 and the moving component 3 are achieved through flexible deformation, and of course, in practice, the sealing effect may be further enhanced by applying a sealant at the connection or adding a sealing ring;

the pipetting push-pull plate 502 is vertically and slidably connected to the lifting plate 401 through a slide rail, the piston rod 601 and the second linear driving assembly are connected to the pipetting push-pull plate 502, the second linear driving assembly can drive the piston rod 601 to move upwards or downwards as required, so that the sample liquid is sucked or extruded, and the second linear driving assembly can be a screw rod, an oil cylinder/an electric push rod and the like.

In the present embodiment of the present utility model,

1. when the device is used, the first linear driving assembly drives the lifting plate 401 to move downwards, so that the slewing mechanism 5 moves downwards until the free end of the connecting assembly 501 passes through the opening of the shell 1 and is connected with the interface 302, at the moment, the bottom end of the connecting head 602 is clamped with the gun head 301, the lifting mechanism 4 continues to drive the slewing mechanism 5 to drive the connecting assembly 501 to move downwards together so as to puncture the film by the gun head 301 and insert the film into the required storage cavity 201, and then the pipetting mechanism 6 sucks sample liquid or discharges the sample liquid into the storage cavity 201; the lifting mechanism 4 drives the moving assembly 3 to move upwards or downwards, and the rotating mechanism 5 drives the moving assembly 3 to rotate circumferentially, so that the sample liquid can move in different storage cavities 201, the whole sequencing process is carried out in a sealed cartridge, and the influence of the environment on the sample liquid is greatly reduced.

2. The slewing mechanism 5 and the lifting mechanism 4 are integrated on the preparation instrument, so that the use cost of the preparation card box serving as consumable materials is reduced; in the use process, the moving assembly 3 is driven to lift and rotate according to the needs only through the slewing mechanism 5 and the lifting mechanism 4, and the structure of the preparation card box and the preparation instrument is more compact, simpler and more convenient and the connection is convenient.

In another embodiment of the present utility model, as shown in fig. 5 and 6, the structure of the preparation apparatus is optimized based on the above embodiment, in order that the cartridge will not move upwards when the connection assembly 501 moves upwards, a pressing mechanism 7 is configured on the rack, the pressing mechanism 7 is used for pressing the cartridge, specifically, the pressing mechanism 7 includes a pressing plate 701 and a third linear driving assembly, where the pressing plate 701 is vertically and slidably connected to the lifting plate 401 through a sliding rail, a avoidance groove for the connection assembly 501 to pass through is provided on the pressing plate 701, the third linear driving assembly is connected to the pressing plate 701, and the third linear driving assembly may be an oil cylinder/air cylinder/electric push rod, etc., and the moving end of the third linear driving assembly moves downwards to drive the pressing plate 701 to move downwards so as to press the top end of the housing 1.

Furthermore, in order to meet the requirement of gene sequencing, the rack is further provided with a heating mechanism for heating the kit 2 and a cooling mechanism 9 for cooling the kit 2, and specifically, the heating mechanism and the cooling mechanism 9 can be an existing peltier heater or cooler.

Further, in order to store the magnetic beads preset in the storage cavity 201 when the pipetting mechanism 6 is pipetting, a magnetic attraction mechanism 8 is further arranged on the rack, the magnetic attraction mechanism 8 is provided with magnets and is distributed outside the reagent kit 2, and the magnetic beads are adsorbed on the wall surface of the reagent kit 2 through magnetic force, so that the magnetic beads are prevented from being sucked away by the pipetting mechanism 6; specifically, the magnet of the magnetic attraction mechanism 8 is arranged on the side surface of the object placing cavity 201, and is driven to move towards the object placing cavity 201 through the fourth linear driving assembly, when the magnet is close to the magnetic beads, the magnetic force is increased, and the adsorption and retention work of the magnetic beads is completed; in addition, can also adsorb through the mode that will adopt the electro-magnet, through the break-make circuit, change the size of magnetic force, electromagnetic means itself is prior art, and this is not repeated to it here.

In another embodiment of the present utility model, as shown in fig. 3 and fig. 4, the main difference between the present utility model and the above embodiment is that a connection manner between one of the interfaces 302 is provided, and for simplicity and convenience, the interface 302 and the connection assembly 501 (corresponding to an external driving mechanism) are connected, where the interface 302 and the connection assembly 501 may be clamped;

specifically, the rod 303 is hollow, the connector 302 is in a notch shape with an open top, and is arranged on the inner wall of the rod 303, and a vertically extending clamping block is arranged on the outer wall of the corresponding positioning column 5015; thus, when the clamping block vertically moves downwards to the insertion interface 302, the power of the slewing mechanism 5 can be transmitted to the moving assembly 3 through the connecting assembly 501, so that the moving assembly 3 synchronously gyrates, the connecting assembly 501 moves downwards, and when the shaft shoulder at the bottom of the connecting assembly is contacted with the top end surface of the moving assembly 3, the moving assembly 3 can be pushed to move downwards; in this way, the structure of the connecting component 501 and the moving component 3 is simple and portable, and the positioning column 5015 is inserted into the rod 303, so that the guiding limit of the moving component 3 is enhanced, and the moving component 3 operates more stably; naturally, if the above connection is adopted, the driving force of the upward movement of the moving assembly 3 is from the elastic assembly; it should be noted that, because the clamping groove 306 of the moving component 3 is clamped with the protruding block 101 at the top of the housing 1 in the initial state, when the moving component 3 needs to rotate, the lifting mechanism 4 drives the connecting component 501 to move downwards, and the clamping groove 306 is already separated from the protruding block 101, so as to avoid motion interference.

In another embodiment of the present utility model, the positioning post 5015 may be made of an elastic material, so that the positioning post 5015 is inserted into the stem 303, and the two are in interference fit, and can also transmit power to the moving component 3 to drive the moving component 3 to rotate circumferentially or move up and down.

Those skilled in the art will appreciate that the embodiments described above are exemplary and that modifications may be made by those skilled in the art, and that the structures described in the various embodiments may be freely combined without conflict in terms of structure or principle. Having described the preferred embodiments of the present utility model in detail, it will be apparent to those skilled in the art that various changes and modifications may be made therein without departing from the scope and spirit of the following claims and that the utility model is not limited to the implementations of the exemplary embodiments set forth in the specification.

Claims (10)

1. A sequencing library preparation cartridge comprising:

a shell (1) with an opening at the top for the driving mechanism to penetrate,

a kit (2) configured in the housing (1) and connected with the housing in a sealing manner, wherein the kit (2) is provided with a plurality of storage cavities (201);

the movable assembly (3) is provided with a gun head (301) and an interface (302), the interface (302) is used for being connected with an external driving mechanism, the movable assembly (3) is rotatably connected in the shell (1) and can move along the axial direction of the shell (1), so that the gun head (301) is alternatively aligned and inserted into the storage cavity (201), and a closed cavity is formed between the movable assembly (3) and the reagent kit (2).

2. A sequencing library preparation cartridge according to claim 1, wherein: the kit comprises a kit body, a kit (2) and a shell (1), wherein the shell (1) is cylindrical, the outer wall of the kit body (2) is attached to the inner wall of the shell (1), and the kit body (2) is provided with a limiting groove which is recessed downwards;

the moving assembly (3) comprises:

one end of the rod part (303) is inserted into the limit groove and can rotate relatively;

and a disc (304) fixedly arranged on the rod part (303), wherein the outer wall of the disc (304) is attached to the inner wall of the shell (1).

3. A sequencing library preparation cartridge as claimed in claim 2, wherein the annular side of the disc (304) is folded down and conforms to the inner wall of the housing (1).

4. A sequencing library preparation cartridge according to claim 3, characterized in that said stem (303) is hollow, said interface (302) is notch-like and it is recessed down on the inner wall of said stem (303).

5. The sequencing library preparing cartridge according to claim 1, wherein the moving assembly (3) is provided with through holes (305) corresponding to the object placing cavities (201) one by one, and the through holes (305) and the object placing cavities (201) are sealed through hot melt films.

6. A sequencing library preparation cartridge according to any one of claims 1 to 5, characterized in that said housing (1) and said moving assembly (3) are provided with positioning modules for positioning said moving assembly (3).

7. The sequencing library preparing cartridge of claim 6, wherein the top of the housing (1) is bent along the center of a circle of the flange, the positioning module comprises a plurality of protrusions (101) which are distributed at the bottom of the flange, the moving assembly (3) is fixedly provided with a clamping groove (306) which can be vertically clamped to the protrusions (101), and the bottom of the housing (1) is provided with an opening.

8. A sequencing library preparation instrument for use in a sequencing library preparation cartridge according to any one of claims 1 to 5, comprising:

the frame is provided with a lifting mechanism (4) and a rotating mechanism (5), the lifting mechanism (4) can drive the rotating mechanism (5) to move up and down, the rotating mechanism (5) is provided with a connecting component (501), the free end of the connecting component (501) is connected with the interface (302) so as to drive the moving component (3) to rotate, and the connecting component (501) is provided with a pushing part which can be abutted against the end face of the moving component (3);

and the pipetting mechanism (6) is vertically and movably connected to the frame through the lifting mechanism (4), and the pipetting mechanism (6) is communicated with the gun head (301) and is used for sucking or extruding sample liquid.

9. The sequencing library preparing apparatus according to claim 8, wherein an elastic member is provided between said connecting member (501) and said kit (2), said elastic member being configured to drive said connecting member (501) to move upward.

10. The sequencing library preparing apparatus of claim 9, wherein said housing is further provided with a pressing mechanism for pressing the cartridge.