CN215906122U - Device for quickly, simply and conveniently extracting protein - Google Patents
Device for quickly, simply and conveniently extracting protein Download PDFInfo
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- CN215906122U CN215906122U CN202121489513.5U CN202121489513U CN215906122U CN 215906122 U CN215906122 U CN 215906122U CN 202121489513 U CN202121489513 U CN 202121489513U CN 215906122 U CN215906122 U CN 215906122U
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Abstract
The utility model discloses a device for quickly, conveniently and simply extracting protein, which comprises an outer pipe, an inner pipe and a filter element, wherein the filter element is detachably arranged in the inner pipe, the upper part of the filter element is in a hollow cylindrical shape with an upward opening, and the lower part of the filter element is in a solid cylindrical shape. The utility model can not only extract protein rapidly, the whole time is about 1-2min, but also the use is more convenient, simultaneously impurities can be filtered out to the utmost extent, the volume of total protein and the protein concentration are not influenced, and great convenience is provided for protein extraction and function research in a laboratory. In addition, due to the adoption of the detachable filter element, the filter element can be repeatedly used, and the production cost of the device is reduced.
Description
Technical Field
The utility model relates to the technical field of experimental devices, in particular to a device for quickly, conveniently and simply extracting protein.
Background
In the course of cell biology experiments, it is a common experimental method to study protein function or compare protein expression differences between different experimental groups and extract proteins from cells or tissues. Conventional extraction of proteins from cells is carried out in four steps: step 1, firstly, discarding culture solution in a culture medium, and washing for 2 times by using PBS; step 2, cell lysis is carried out on the cell lysate, and protein is released from the cells; step 3, centrifuging for 15-20 minutes at 4 ℃ and 12000 rpm; and 4, taking the centrifuged supernatant, transferring the centrifuged supernatant into a new centrifugal tube, and measuring the concentration of the protein. However, in the actual operation process, the following problems are found in step 3: the process takes 15-20 minutes for centrifugation with a centrifuge. Secondly, after the centrifugation is finished, floccules may be suspended in the supernatant or impurities with lower density may also be suspended on the surface and cannot be separated from the supernatant, so that the subsequent determination of the protein concentration is influenced.
Aiming at the defect of extracting protein from cells or tissues at present, a novel protein extraction device is designed, the device can reduce the residues of flocculent suspended matters and impurities with lower density in supernatant, can remove precipitates such as cell fragments and the like which can be removed during high-speed centrifugation, can remove the impurities to the maximum extent, does not influence the total volume of the protein and the concentration of the protein during filtration, improves the accuracy of protein concentration determination, can realize the separation of the protein sample impurities at low speed, can meet the requirements of laboratories without low-temperature high-speed centrifuges, has the whole experimental process of about 1-2 minutes, saves much time compared with the conventional extraction method, and greatly improves the working efficiency.
Chinese utility model patent (No. CN 212293386U) discloses an automatic extraction element of cell components, including liquid-transfering system, filtration system, cooling system. Wherein the filtering system comprises a filter tube, and the filter tube comprises a filter column, a collecting tube and a filter element. The filter column comprises a filter element, the filter element breaks the cell sensitized by the cell membrane and filters the nucleic acid DNA in the process of pressurized filtration of the compressed gas, and the obtained filtrate is other cell components without DNA. The utility model discloses a shortcoming lies in: the filter element is not easy to be disassembled in the filter column, and the filter column needs to be replaced every time of use, thereby increasing the cost. The device is relatively complex and has high requirements for laboratories. In the use of the whole device, experimental articles such as nitrogen are required to be used, the use cost of equipment is increased, and the device is not easy to popularize.
Chinese patent application publication No. CN 102939524 a discloses a biological sample pretreatment method and apparatus, the apparatus includes a filter member and a filter device for filtration, and a solid phase extraction device connected with the filter device and having a solid phase extraction agent. The apparatus for treating a biological sample requires a filter device disposed on the upstream side and a solid phase extraction device disposed on the downstream side for the test. The utility model patent application has the following defects: the filter equipment needs to be matched with a solid phase extraction equipment for use, the required substances cannot be directly obtained after filtration, the biological sample is still treated by adopting a centrifugal separation mode after stirring, the experimental process cannot be accelerated, and the target object can be extracted without equipment such as a centrifugal machine.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned defects of the prior art, the technical problems to be solved by the present invention are that the time consumption of the existing cell protein extraction technology is too long, the impurities are too much after the protein extraction, the protein concentration measurement is inaccurate, and the cost is high. These problems can cause low experimental efficiency and serious error of experimental results. The utility model provides a device for quickly, conveniently and simply extracting protein, which can reduce the time for extracting the protein, remove impurities to the maximum extent, improve the accuracy of subsequent protein concentration determination, greatly improve the working efficiency and reduce the cost.
In order to solve the technical problems, the utility model provides the following technical scheme:
the utility model provides a swift simple and convenient device that draws protein, includes outer tube, inner tube and filter core, the inner tube be located the outer tube, filter core detachable install in the inner tube, filter core upper portion be the cavity circular cylinder that the opening is up, the lower part is solid cylindrical.
In the device for quickly, conveniently and simply extracting the protein, the outer pipe is a hollow pipe with a conical bottom and a cylindrical middle upper end, and is mainly used for collecting the filtered protein liquid.
In the device for quickly, conveniently and simply extracting the protein, the upper end of the inner wall of the outer tube is protruded. The protrusions may be two pairs of two parallel protrusions.
The device for quickly, conveniently and simply extracting the protein is characterized in that the inner pipe is a hollow cylindrical pipe with an upper opening and a lower opening.
The device for quickly, conveniently and simply extracting the protein is characterized in that the lower end of the inner wall of the inner pipe is provided with the supporting structure, and the supporting structure is mainly used for preventing the filter element from falling off from the inner pipe during centrifugation. The support structure may be a cross-shaped support structure.
According to the device for quickly, conveniently and simply extracting the protein, the inner wall of the inner pipe is provided with the protrusion, the protrusion is used for fixing the filter element, the upper part of the upper end of the filter element is provided with the recess matched with the protrusion of the inner wall of the inner pipe, the recess is used for being matched with the protrusion of the inner wall of the inner pipe for fixing the filter element, the filter element is prevented from falling off, and supernatant is also prevented from flowing to the outer pipe from the side wall.
According to the device for quickly, conveniently and simply extracting the protein, the outer wall of the inner pipe is provided with the protrusion, the protrusion is matched with the protrusion of the inner wall of the outer pipe, the inner pipe is fixed in the outer pipe, the inner pipe is prevented from falling off, meanwhile, the inner pipe can be conveniently taken out of the outer pipe, and the filtered liquid in the outer pipe is moved to other containers.
According to the device for quickly, conveniently and simply extracting the protein, the height of the solid cylinder at the lower part of the filter element is 0.5 cm. The height of the lower solid cylinder is preferably 0.5cm, which is the result of a number of tests carried out by us. If the filter element is too short, impurities cannot be completely removed, and the filter element is too long, so that the filter element occupies space.
The steps when in use are as follows:
s1: discarding culture solution in cells, washing for 2 times by using PBS, and performing cell lysis by using cell lysis solution;
s2: transferring the liquid after cracking to an inner tube of a protein extraction device, and enabling the liquid to pass through a filter element and be filtered;
s3: collecting the filtered liquid in the outer tube, and moving the liquid to a new protein extraction device again;
s4: the protein concentration was measured.
The technical scheme provided by the device for quickly, conveniently and simply extracting the protein has the following technical effects:
when the device for quickly, conveniently and simply extracting the protein is used for carrying out a protein extraction experiment, the protein can be quickly extracted, the whole process is about 1-2 minutes, impurities can be filtered out to the maximum extent, the volume of total protein and the protein concentration are not influenced, compared with the conventional extraction method, the experimental time can be saved, and the working efficiency is greatly improved. Meanwhile, the residues of flocculent suspended matters and impurities with low density in the supernatant can be reduced, and precipitates such as cell debris and the like which can be removed only during high-speed centrifugation are removed, so that the impurities are removed to the maximum extent. The device can realize the separation of protein sample impurities at low speed, can meet the requirements of laboratories without low-temperature high-speed centrifuges, does not influence the total volume of protein and the concentration of the protein during filtration, and improves the accuracy of protein concentration determination. In addition, due to the adoption of the detachable filter element, the filter element can be repeatedly used, and the production cost of the device is reduced.
Compared with the Chinese utility model patent (grant publication No. CN 212293386U), the purpose of the comparison patent is to make the cell disruption more uniform and reduce the difference caused by manual operation during the cell extraction process. The utility model aims to quickly extract protein from supernatant after cell lysis, reduce impurities to the maximum extent, and prevent the volume and concentration of total protein from being influenced, and the technical problems of the two methods are obviously different. The device is simple, has low requirements on equipment and environment in a laboratory, has low use cost, simple and convenient use method and wide application range, is suitable for extracting protein of cells and tissues, is also suitable for filtering serum, and can be popularized and used in a large range.
Compared with the Chinese patent application (publication No. CN 102939524A), the purpose of the patent is to remove protein in the filtrate, and the utility model is to extract protein, and the technical problems solved by the two are obviously different.
Compared with the two patents, the utility model is different in that: the filter element is a detachable filter element, the upper part of the filter element is a hollow cylinder with an upward opening, and the lower part of the filter element is a solid cylinder. The supernatant passes through the upper hollow cylinder to reach the lower solid cylinder, and the design can prevent the supernatant from flowing into the outer pipe from the space between the filter element and the inner pipe during centrifugation. The lower solid cylinder has a height of 0.5cm, which is the result of extensive experimental tests carried out by us. If the filter element is too short, impurities cannot be completely removed, and the filter element is too long, so that the filter element occupies space. At the lower part of inner tube, be equipped with bearing structure, this bearing structure can prevent that the filter core from dropping when the centrifugation, is favorable to the supernatant to leak down from the top simultaneously.
Drawings
The conception, the specific structure and the technical effects of the present invention will be further described with reference to the accompanying drawings to fully understand the objects, the features and the effects of the present invention.
FIG. 1 is a schematic structural diagram of an apparatus for rapid and convenient protein extraction according to the present invention;
FIG. 2 is a schematic view of the structure of the outer tube of the apparatus for rapid and convenient extraction of protein according to the present invention;
FIG. 3 is a schematic diagram of the inner tube of the apparatus for rapid and convenient protein extraction according to the present invention;
FIG. 4 is a schematic diagram of a filter element structure of the device for rapidly and conveniently extracting protein according to the present invention.
Wherein the reference numbers are as follows:
the filter comprises an outer pipe 1, a protrusion 1-1 on the inner wall of the outer pipe, an inner pipe 2, a protrusion 2-1 on the inner wall of the inner pipe, a protrusion 2-2 on the outer wall of the inner pipe, a cross-shaped supporting structure 2-3 at the lower end of the inner wall of the inner pipe, a filter element 3 and a recess 3-1 on the upper part of the upper end of the filter element.
Detailed Description
In order to make the technical means, the characteristics, the purposes and the functions of the utility model easy to understand, the utility model is further described with reference to the specific drawings. However, the present invention is not limited to the following embodiments.
It should be understood that the structures, ratios, sizes, and the like shown in the drawings and described in the specification are only used for matching with the disclosure of the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions under which the present invention can be implemented, so that the present invention has no technical significance, and any structural modification, ratio relationship change, or size adjustment should still fall within the scope of the present invention without affecting the efficacy and the achievable purpose of the present invention.
In addition, the terms such as "inner", "outer", "upper" and "lower" used in the present specification are for clarity of description, and are not intended to limit the scope of the present invention, and changes or modifications in the relative relationship may be made without substantial changes in the technical content.
The utility model provides a device for simply, conveniently and quickly extracting protein, and aims to not only quickly extract protein and save experimental time, but also filter impurities to the maximum extent when in use, and do not influence the volume and the protein concentration of total protein, thereby providing great convenience for laboratory protein extraction and function research.
As shown in figure 1, the device for quickly, conveniently and simply extracting protein comprises an outer tube 1, an inner tube 2 and a filter element 3, wherein the filter element 3 is positioned in the inner tube 2, and the inner tube 2 is positioned in the outer tube 1. Wherein, filter core 3 upper portion is the cavity cylindrical that the opening is up, and the lower part is solid cylindrical, and filter core 3 detachable installs in inner tube 2.
As shown in fig. 2, the outer tube 1 is a hollow tube with a conical bottom and a cylindrical middle upper end, and mainly collects the filtered protein solution. Wherein, the protrusions 1-1 at the upper end of the inner wall of the outer tube 1 can be two pairs of protrusions which are parallel to each other.
As shown in fig. 3, the inner tube 2 is a hollow cylindrical tube opened at the upper and lower sides. The inner wall of the inner tube 2 is provided with a protrusion 2-1, and the protrusion 2-1 is used for matching with the recess 3-1 of the filter element 3, so that the filter element 3 (shown in figure 4) is fixed conveniently and liquid is prevented from flowing into the side wall of the inner tube 2. The outer wall of the inner pipe 2 is provided with a protrusion 2-2, and the purpose of the protrusion 2-2 is to be matched with the protrusion 1-1 on the inner wall of the outer pipe to fix the inner pipe 2 together, so that the inner pipe 2 is prevented from falling (see fig. 2 and fig. 1, the protrusion 2-2 on the outer wall of the inner pipe is just clamped on the protrusion 1-1 on the inner wall of the outer pipe to fix the inner pipe 2 in the outer pipe 1, and the inner pipe 2 is prevented from falling), and meanwhile, the inner pipe 2 can be conveniently taken out from the outer pipe 1, and filtered liquid of the outer pipe 1 is moved to other containers. The inner tube 2 is a hollow cylindrical tube with an upper opening and a lower opening, the lower end of the inner wall of the inner tube 2 is provided with a cross-shaped supporting structure 2-3, the main function is to bear the filter element 3 inside, the filter element 3 is prevented from falling off during centrifugation, and meanwhile, the upper surface of the lower surface of the inner tube 2 is beneficial to the leakage of supernatant.
As shown in FIG. 4, the upper portion of the upper end of the filter element 3 is provided with a recess 3-1, and the recess 3-1 is engaged with the protrusion 2-1 of the inner wall of the inner tube to prevent the filter element 3 from falling down and also to prevent the supernatant from flowing from the side wall to the outer tube 1. The upper part of the filter element 3 is a hollow cylinder with an upward opening, the lower part of the filter element 3 is a solid cylinder, the height of the solid cylinder at the lower part of the filter element 3 is preferably 0.5cm, and the height of 0.5cm is the result of a large number of experimental tests. If the filter element 3 is too short, the impurities cannot be completely removed, and if the filter element 3 is too long, the space is occupied.
The steps of the protein extraction by using the rapid and simple protein extraction device are as follows:
s1: discarding the culture solution in the cells, washing twice by using PBS, and cracking the cells by using cell lysis solution;
s2: transferring the liquid after cracking to an inner tube 2 of a protein extraction device, and enabling the liquid to pass through a filter element 3 to be filtered;
s3: collecting the filtered liquid in the outer tube 1, and moving the liquid to a new protein extraction device again;
s4: the protein concentration was measured.
To sum up, the device for quickly, simply and conveniently extracting the protein can reduce the residues of flocculent suspended matters and impurities with lower density in the supernatant, can remove precipitates such as cell debris and the like which can be removed only during high-speed centrifugation, can remove the impurities to the maximum extent, does not influence the total volume of the protein and the concentration of the protein during filtration, improves the accuracy of protein concentration determination, can realize the separation of the impurities of the protein sample at low speed, can meet the requirements of laboratories without low-temperature high-speed centrifuges, saves a lot of time compared with a conventional extraction method and greatly improves the working efficiency in the whole experimental process for about 1-2 minutes. In addition, due to the adoption of the detachable filter element, the filter element can be repeatedly used, and the production cost of the device is reduced.
The foregoing detailed description of the preferred embodiments of the utility model has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.
Claims (10)
1. The utility model provides a swift simple and convenient device that draws protein, its characterized in that includes the outer tube, inner tube and filter core, the inner tube be located the outer tube, filter core detachable install in the inner tube, filter core upper portion be the cavity cylindrical that the opening is up, the lower part is solid cylindrical.
2. A rapid and simple device for extracting protein according to claim 1, wherein the outer tube is a hollow tube with a conical bottom and a cylindrical middle upper end.
3. A rapid and simple protein extraction device as claimed in claim 2, wherein the upper end of the inner wall of the outer tube is protruded.
4. A rapid and convenient protein extraction device as claimed in claim 3, wherein the upper end of the inner wall of the outer tube is protruded into two pairs of protrusions parallel to each other.
5. The apparatus for rapid and convenient extraction of protein according to claim 1, wherein the inner tube is a hollow cylindrical tube with an open top and bottom.
6. A rapid and simple protein extraction device as claimed in claim 5, wherein the inner wall of the inner tube has a support structure at its lower end.
7. The device for rapidly and conveniently extracting protein according to claim 6, wherein the supporting structure arranged at the lower end of the inner wall of the inner tube is a cross-shaped supporting structure.
8. A rapid and simple protein extraction apparatus as claimed in claim 6, wherein the inner wall of the inner tube has protrusions, and the upper part of the upper end of the filter element has recesses matching the protrusions of the inner wall of the inner tube.
9. A rapid and simple protein extraction device according to claim 3 or 4, characterized in that the outer wall of the inner tube has protrusions, and the protrusions of the outer wall of the inner tube are matched with the protrusions of the inner wall of the outer tube to fix the inner tube.
10. A rapid and simple device for extracting protein according to claim 1, wherein the height of the solid cylinder at the lower part of the filter element is 0.5 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121489513.5U CN215906122U (en) | 2021-07-02 | 2021-07-02 | Device for quickly, simply and conveniently extracting protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121489513.5U CN215906122U (en) | 2021-07-02 | 2021-07-02 | Device for quickly, simply and conveniently extracting protein |
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| Publication Number | Publication Date |
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| CN215906122U true CN215906122U (en) | 2022-02-25 |
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| CN202121489513.5U Active CN215906122U (en) | 2021-07-02 | 2021-07-02 | Device for quickly, simply and conveniently extracting protein |
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- 2021-07-02 CN CN202121489513.5U patent/CN215906122U/en active Active
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