CN220894320U - Immunological detection kit for flow type fluorescence detection method - Google Patents
Immunological detection kit for flow type fluorescence detection method Download PDFInfo
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- CN220894320U CN220894320U CN202321441349.XU CN202321441349U CN220894320U CN 220894320 U CN220894320 U CN 220894320U CN 202321441349 U CN202321441349 U CN 202321441349U CN 220894320 U CN220894320 U CN 220894320U
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- suction filtration
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- pore plate
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- 238000001514 detection method Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 12
- 230000001900 immune effect Effects 0.000 title claims abstract description 11
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 79
- 239000011148 porous material Substances 0.000 claims abstract description 33
- 238000000967 suction filtration Methods 0.000 claims abstract description 32
- 239000004005 microsphere Substances 0.000 claims abstract description 19
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000011534 wash buffer Substances 0.000 claims abstract description 12
- 239000000243 solution Substances 0.000 claims abstract description 10
- 239000003085 diluting agent Substances 0.000 claims abstract description 9
- 239000012528 membrane Substances 0.000 claims description 17
- 239000012086 standard solution Substances 0.000 claims description 7
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 6
- 239000002033 PVDF binder Substances 0.000 claims description 4
- -1 polytetrafluoroethylene Polymers 0.000 claims description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 4
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 4
- 238000005558 fluorometry Methods 0.000 claims 6
- 238000003018 immunoassay Methods 0.000 claims 6
- 238000011534 incubation Methods 0.000 abstract description 5
- 229920001184 polypeptide Polymers 0.000 abstract description 5
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 5
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 238000000338 in vitro Methods 0.000 abstract description 4
- 239000012898 sample dilution Substances 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 24
- 238000004140 cleaning Methods 0.000 description 7
- 238000001914 filtration Methods 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Abstract
The utility model discloses an immunological detection kit for a flow type fluorescence detection method, which comprises a box body, wherein a suction filtration pore plate and six reagents are arranged in the box body, and the reagents comprise a capture microsphere reagent, a standard liquid, a detection secondary antibody reagent, a fluorescent dye reagent, a sample diluent and a washing buffer solution; the kit further comprises a reagent rack, wherein a plurality of reagent bottle positions are arranged on the reagent rack, are respectively a microsphere capturing reagent position, a standard liquid level, a secondary antibody detecting reagent position, a fluorescent dye reagent position, a sample dilution liquid level and a washing buffer liquid level, and are used for placing six reagents. The utility model can realize the in vitro incubation of protein and polypeptide substances, and can be quickly cleaned after the in vitro incubation is completed, so that the protein and polypeptide substances can be sent to a flow cytometer for machine detection.
Description
Technical Field
The utility model relates to the technical field of detection kits, in particular to an immunological detection kit for a flow fluorescent detection method.
Background
The flow type fluorescence technology has the characteristics of high flux, high speed, simple operation and the like, can detect a plurality of indexes at one time, can realize batch processing of samples, and is widely applied to the fields of medical inspection and life science. The method comprises the steps of detecting proteins, polypeptides and derivatives thereof in various biological fluid samples by a flow type fluorescence detection method, wherein the biological samples are firstly incubated, including the steps of adding specific capture microspheres and detection antibodies for incubation-cleaning, adding fluorescent dyes for incubation-cleaning. During the incubation and washing processes, a filter is required to filter the sample. In the existing detection kit, a centrifugal method is generally adopted to perform reaction and cleaning in a test tube, a liquid shifter is needed to remove waste liquid after centrifugation, and detection microspheres and supernatant liquid are easily sucked away in the process, so that the loss of the detection microspheres is reduced, and the detection result is influenced. Therefore, it is necessary to provide an immunological detection kit that is more convenient and efficient to use.
Disclosure of utility model
In order to solve the defects in the prior art, the utility model provides an immunological detection kit for a flow type fluorescence detection method, which is convenient for incubating and rapidly cleaning a sample before detection.
In order to achieve the technical purpose, the utility model adopts the following technical scheme:
An immunological detection kit for a flow type fluorescence detection method comprises a box body, wherein a suction filtration pore plate and six reagents are arranged in the box body, and the reagents comprise a capture microsphere reagent, a standard solution, a detection secondary antibody reagent, a fluorescent dye reagent, a sample diluent and a washing buffer solution; the kit further comprises a reagent rack, wherein a plurality of reagent bottle positions are arranged on the reagent rack, are respectively a microsphere capturing reagent position, a standard liquid level, a secondary antibody detecting reagent position, a fluorescent dye reagent position, a sample dilution liquid level and a washing buffer liquid level, and are used for placing six reagents.
Further, the suction filtration pore plate comprises a vacuum suction filtration plate main body, a plurality of sample reaction holes are arranged in the vacuum suction filtration plate main body, and a filtration membrane is arranged at the bottom of each sample reaction hole.
Further, the main body of the vacuum filter plate is made of hydrophilic polyvinylidene fluoride or styrene.
Further, the volume of the sample reaction well is 250-300. Mu.L.
Further, the filter membrane is made of polytetrafluoroethylene membrane.
Further, the pore size of the filter membrane is 1+ -0.5 μm.
Further, the suction filtration pore plate is a 96 pore plate and is provided with 8.12 sample reaction holes.
Further, a suction filtration pore plate clamping groove is further formed in one side of the reagent frame.
The utility model has the beneficial effects that:
The immunological detection kit comprises a suction filtration pore plate, a capturing microsphere reagent, a standard solution, a detection secondary antibody reagent, a fluorescent dye reagent, a sample diluent and a washing buffer solution, and can realize rapid cleaning after in-vitro incubation of protein and polypeptide substances is completed so as to be sent to a flow cytometry for machine-on-machine testing.
Under the suction action of the vacuum suction filtration device, the filter membrane can well retain microspheres with diameters of more than 2.5 mu m in the sample reaction hole, and other reagent components and residual samples can smoothly pass through the filter membrane to rapidly finish filtration and cleaning.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the structure of a reagent rack in the present utility model;
fig. 2 is a schematic structural view of a suction filtration pore plate in the present utility model.
Reference numerals: 1. the clamping groove of the suction filtration pore plate; 2. capturing microsphere reagent sites; 3. standard liquid level; 4. detecting the secondary antibody reagent position; 5. fluorescent dye reagent site; 6. sample diluent reagent site; 7. washing buffer solution reagent positions, 8, and vacuumizing a filter plate main body; 9. a sample reaction well; 10. and (5) a filter membrane.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. The components of the embodiments of the present application generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the application, as presented in the figures, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The utility model provides an immunological detection kit for a flow type fluorescence detection method, which is shown in fig. 1 and 2, and comprises a box body, wherein a suction filtration pore plate and six reagents are arranged in the box body, and the reagents comprise a capture microsphere reagent, a standard solution, a detection secondary antibody reagent, a fluorescent dye reagent, a sample diluent and a washing buffer solution; the kit further comprises a reagent rack, wherein a plurality of reagent bottle positions are arranged on the reagent rack as shown in fig. 2, namely a capturing microsphere reagent position 2, a standard liquid reagent position 3, a detection secondary antibody reagent position 4, a fluorescent dye reagent position 5, a sample diluent reagent position 6 and a washing buffer reagent position 7, and the kit is used for placing six reagents.
The suction filtration pore plate comprises a vacuum suction filtration plate main body 8, a plurality of sample reaction holes 9 are arranged in the vacuum suction filtration plate main body 8, and a filter membrane 10 is arranged at the bottom of each sample reaction hole 9; the vacuum filter plate main body 8 is made of hydrophilic polyvinylidene fluoride (PVDF) or styrene; the volume of the sample reaction hole 9 is 250-300 mu L; the filter membrane is made of polytetrafluoroethylene membrane, and the pore diameter is 1+/-0.5 mu m. Preferably, the suction filtration pore plate is a 96-pore plate, and 8×12 sample reaction pores 9 are arranged.
Further, a suction filtration pore plate clamping groove position 1 is formed in one side of the reagent frame, and the suction filtration pore plate is clamped in the suction filtration pore plate clamping groove position 1.
The application process of the utility model is as follows: taking out the suction filtration pore plate and the reagent frame, wherein the suction filtration pore plate is clamped in the clamping groove of the suction filtration pore plate; taking a certain amount of capture microsphere reagent and detection secondary antibody reagent, adding the capture microsphere reagent and the detection secondary antibody reagent into a sample reaction hole of a suction filtration pore plate, and adding a certain amount of biological sample or standard solution; incubating for a predetermined time at a preset temperature and oscillation frequency, and filtering out unbound residual reagent and biological sample (or standard solution) in the sample reaction well by using a vacuum filtering device (not shown in the figure); adding a fluorescent dye reagent, incubating for a certain time, and washing and removing unbound residual fluorescent dye reagent in a sample reaction hole by using a vacuum suction filtration device; finally, the microspheres trapped in the sample reaction well are resuspended by a certain volume of washing buffer, and the concentration of the specific substances in the sample is detected on a flow cytometer.
The immunological detection kit comprises a suction filtration pore plate, a capturing microsphere reagent, a standard solution, a detection secondary antibody reagent, a fluorescent dye reagent, a sample diluent and a washing buffer solution, and can realize rapid cleaning after in-vitro incubation of protein and polypeptide substances is completed so as to be sent to a flow cytometry for machine-on-machine testing. The filter membrane of the suction filtration pore plate adopts a polytetrafluoroethylene membrane, the pore diameter is 1+/-0.5 mu m, microspheres with the diameter of more than 2.5 mu m can be well trapped in the sample reaction pore, and other reagent components and residual samples can smoothly pass through the filter membrane.
Of course, the present utility model is capable of other various embodiments and its several details are capable of modification and variation in light of the present utility model by one skilled in the art without departing from the spirit and scope of the utility model as defined in the appended claims.
Claims (7)
1. An immunological detection kit for a flow type fluorescence detection method, which comprises a box body and is characterized in that: the kit is internally provided with a suction filtration pore plate and six reagents, wherein the reagents comprise a capturing microsphere reagent, a standard solution, a detection secondary antibody reagent, a fluorescent dye reagent, a sample diluent and a washing buffer solution; the kit further comprises a reagent rack, wherein a plurality of reagent bottle positions are arranged on the reagent rack, namely a microsphere capturing reagent position, a standard liquid reagent position, a secondary antibody detecting reagent position, a fluorescent dye reagent position, a sample diluent reagent position and a washing buffer reagent position, and are used for placing six reagents; the suction filtration pore plate comprises a vacuum suction filtration plate main body, a plurality of sample reaction holes are arranged in the vacuum suction filtration plate main body, and a filter membrane is arranged at the bottom of each sample reaction hole.
2. The immunoassay kit for flow fluorometry of claim 1, wherein: the main body of the vacuum filter plate is made of hydrophilic polyvinylidene fluoride or styrene.
3. The immunoassay kit for flow fluorometry of claim 1, wherein: the volume of the sample reaction well is 250-300 mu L.
4. The immunoassay kit for flow fluorometry of claim 1, wherein: the filter membrane is made of polytetrafluoroethylene membrane.
5. The immunoassay kit for flow fluorometry of claim 4, wherein: the pore diameter of the filter membrane is 1+/-0.5 mu m.
6. The immunoassay kit for flow fluorometry of claim 1, wherein: the suction filtration pore plate is a 96 pore plate and is provided with 8 x 12 sample reaction holes.
7. The immunoassay kit for flow fluorometry of claim 1, wherein: and one side of the reagent rack is also provided with a suction filtration pore plate clamping groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321441349.XU CN220894320U (en) | 2023-06-07 | 2023-06-07 | Immunological detection kit for flow type fluorescence detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321441349.XU CN220894320U (en) | 2023-06-07 | 2023-06-07 | Immunological detection kit for flow type fluorescence detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220894320U true CN220894320U (en) | 2024-05-03 |
Family
ID=90868129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321441349.XU Active CN220894320U (en) | 2023-06-07 | 2023-06-07 | Immunological detection kit for flow type fluorescence detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220894320U (en) |
-
2023
- 2023-06-07 CN CN202321441349.XU patent/CN220894320U/en active Active
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