CN214032447U - A filter equipment for oocyte - Google Patents
A filter equipment for oocyte Download PDFInfo
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- CN214032447U CN214032447U CN202022607455.3U CN202022607455U CN214032447U CN 214032447 U CN214032447 U CN 214032447U CN 202022607455 U CN202022607455 U CN 202022607455U CN 214032447 U CN214032447 U CN 214032447U
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Abstract
The utility model belongs to the technical field of experimental apparatus structures, and discloses a filtering device for oocytes, wherein a liquid feeding fixed pool is clamped at the lower end of a sample liquid feeding pool, and a waste liquid bottle is clamped at the lower end of the liquid feeding fixed pool; the upper end of the specimen liquid adding pool is open, and the lower end of the specimen liquid adding pool is provided with a filtering membrane; the lower end of the liquid feeding fixed pool is provided with a filtering membrane; the lower end of the waste liquid bottle is integrally provided with a partition plate, the lower side of the partition plate is provided with an electric negative pressure device, and one side of the electric negative pressure device is hermetically connected with the upper end of the waste liquid bottle through a connecting pipe; the filter pore diameter of the filter membrane is 2-20 μm. The antibody incubation and multiple cleaning operations can be completed in the filtering membrane 4, multiple transfer of oocytes is avoided, the operation conditions and the processing flow are unified, the flow time is shortened, the loss rate of the cells during multiple transfer is reduced, and the operation is convenient and rapid.
Description
Technical Field
The utility model belongs to the technical field of utensil structure for the experiments, especially, relate to a filter equipment for oocyte.
Background
At present, immunofluorescence staining operation is often performed on oocytes in biological science research, in the antibody incubation, staining marking and cleaning process of a traditional oocyte in-vitro immunofluorescence experiment, the oocytes are often transferred from one culture drop to other culture drops by using a mouth suction tube, the oocytes are incubated in different reaction liquids (such as a first antibody, a fluorescence-marked second antibody and the like) and cleaned in different cleaning liquids to form an antigen-antibody complex and a fluorescein mark, a fluorescence microscope and a confocal microscope are used for observing oocyte specimens, and the fluorescein emits bright fluorescence under the irradiation of excitation light, so that the properties and the positioning of the antigen or the antibody are determined, and the content is determined by using a quantitative technology. In the processes of obtaining and cleaning oocytes, forming antigen-antibody complexes, labeling fluorescein and fixing, transferring, incubating and cleaning for several times, the operation process is complicated and time-consuming, the loss rate is increased, and the oocytes in the same batch can have differences in factors such as reagents, operation and processing time. In the traditional operation method, the immunofluorescence staining operation flow of the oocyte is observed and operated under a body type microscope, and the whole process is completed by repeatedly transferring in a pore plate and sucking an egg sucking needle according to the proficiency and experience of an operator, so that the operator needs to carefully adjust the proper pressing pressure, and the difficulty of successful operation is increased.
Through the above analysis, the problems and defects of the prior art are as follows: in the prior art, operators need to carefully adjust the proper pressing force, and the operation difficulty is high.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems existing in the prior art, the utility model provides a filtering device for oocytes.
The utility model discloses a realize like this, a filter equipment for oocyte is provided with:
sample liquid feeding pond:
the lower end of the sample liquid adding pool is clamped with a liquid adding fixing pool, and the lower end of the liquid adding fixing pool is clamped with a waste liquid bottle;
the upper end of the specimen liquid adding pool is open, and the lower end of the specimen liquid adding pool is provided with a filtering membrane; and a filtering membrane is arranged at the lower end of the liquid adding fixing pool.
Further, a partition board is integrally arranged at the lower end of the waste liquid bottle, an electric negative pressure device is arranged on the lower side of the partition board, and one side of the electric negative pressure device is hermetically connected with the upper end of the waste liquid bottle through a connecting pipe.
Furthermore, the filter pore diameter of the filter membrane is 2-20 μm.
Further, sample liquid feeding pond lower extreme is provided with first boss, the fixed pond upper end of liquid feeding be provided with first boss assorted first recess, first boss with be provided with first sealed pad between the first recess.
Furthermore, a second boss is arranged at the lower end of the liquid adding fixing pool, a second groove matched with the second boss is arranged at the upper end of the waste liquid bottle, and a second sealing gasket is arranged between the second boss and the second groove.
Furthermore, a bottom plate is clamped at the bottom of the waste liquid bottle, and a plurality of through holes are formed in the bottom plate.
Combine foretell all technical scheme, the utility model discloses the advantage that possesses and positive effect are:
the utility model discloses can accomplish the antibody and hatch and wash the operation many times in filtering the membrane, avoid oocyte's repetitious transfer, unify operating condition and processing flow to shorten the flow time, reduced the loss rate when cell repetitious transfer, convenient operation is swift.
When parallel experiments are carried out, the same reagent and the same processing flow are adopted, and the quality control is easy. Operations such as oocyte is hatched and is washd can't accomplish in traditional operation in step, all need to handle every batch of oocyte alone, separate timing, and the processing time is often just roughly the same, can't be unanimous, and every batch of oocyte all accomplishes in the operation liquid drop of difference, therefore can influence experimental data. The oocyte immunofluorescence staining process is standardized, the operation of fully relying on the experience of hand feeling is avoided, and the oocyte immunofluorescence staining is unified, so that the staining effect is uniform.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a schematic structural view of a filtering device for oocytes according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a filtering membrane and a first boss provided in an embodiment of the present invention;
FIG. 3 is a schematic structural view of a liquid feeding stationary pool and a first groove provided in an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a second groove and a bottom plate provided in the embodiment of the present invention;
in the figure: 1. a specimen liquid adding pool; 2. a liquid adding fixing pool; 3. a connecting pipe; 4. a filtration membrane; 5. filtering through a membrane; 6. a waste liquid bottle; 7. an electric negative pressure device; 8. a first boss; 9. a first groove; 10. a second boss; 11. a second groove; 12. a base plate.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
To the problems existing in the prior art, the utility model provides a filter equipment for oocyte, it is right to combine the figure below the utility model discloses do detailed description.
As shown in fig. 1 to 4, the present embodiment provides a filtering apparatus for oocytes, comprising: the device comprises a specimen liquid adding pool 1, a liquid adding fixing pool 2, a connecting pipe 3, a filtering membrane 4, a filtering membrane 5, a waste liquid bottle 6, an electric negative pressure device 7, a first boss 8, a first groove 9, a second boss 10, a second groove 11 and a bottom plate 12.
The lower end of the specimen liquid adding pool 1 of the embodiment is clamped with a liquid adding fixed pool 2, and the lower end of the liquid adding fixed pool 2 is clamped with a waste liquid bottle 6; the upper end of the sample liquid adding pool 1 is open, and the lower end is provided with a filtering membrane 4; the lower end of the liquid adding fixing pool 2 is provided with a filtering membrane 5; the antibody incubation and multiple cleaning operations can be completed in the filtering membrane 4, multiple transfer of oocytes is avoided, the operation conditions and the processing flow are unified, the flow time is shortened, the loss rate of the cells during multiple transfer is reduced, and the operation is convenient and rapid.
In this embodiment, the lower end of the waste liquid bottle 6 is integrally provided with a partition board, the lower side of the partition board is provided with an electric negative pressure device 7, and one side of the electric negative pressure device 7 is hermetically connected with the upper end of the waste liquid bottle 6 through a connecting pipe 3.
In this embodiment, the filter pore diameter of the filter membrane 5 is 2 to 20 μm; under normal atmospheric pressure conditions, the liquid cannot pass through the filter membrane 5 by gravity, so the filter normally behaves as a sample tank for addition of oocytes and test reagents.
In this embodiment, 1 lower extreme in sample liquid feeding pond is provided with first boss 8, 2 upper ends in the fixed pond of liquid feeding be provided with first boss 8 assorted first recess 9, be provided with first sealed pad between first boss 8 and the first recess 9. The lower end of the liquid adding fixing pool 2 is provided with a second boss 10, the upper end of the waste liquid bottle 6 is provided with a second groove 11 matched with the second boss 10, and a second sealing gasket is arranged between the second boss 10 and the second groove 11. The airtightness of the whole device can be ensured.
In this embodiment, a bottom plate 12 is clamped at the bottom of the waste liquid bottle 6, and a plurality of through holes are arranged on the bottom plate 12.
The utility model discloses a theory of operation does: firstly, a specimen liquid adding pool 1, a liquid adding fixed pool 2 and a waste liquid bottle 6 are hermetically connected, oocytes of an experimental group are respectively sucked into the specimen liquid adding fixed pool 2, and required staining liquid and cleaning liquid are sequentially injected into the specimen fixed liquid adding pool to complete subsequent operations such as antibody incubation and cleaning; and transferring the incubated and cleaned oocyte onto a glass slide, and mounting the glass slide to carry out microscopic observation.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be covered within the protection scope of the present invention by those skilled in the art within the technical scope of the present invention.
Claims (6)
1. A filter device for oocytes, characterized in that it is provided with:
sample liquid feeding pond:
the lower end of the sample liquid adding pool is clamped with a liquid adding fixing pool, and the lower end of the liquid adding fixing pool is clamped with a waste liquid bottle;
the upper end of the specimen liquid adding pool is open, and the lower end of the specimen liquid adding pool is provided with a filtering membrane; and a filtering membrane is arranged at the lower end of the liquid adding fixing pool.
2. The oocyte filter device according to claim 1, wherein a partition plate is integrally provided at a lower end of the waste liquid bottle, an electric negative pressure device is provided at a lower side of the partition plate, and one side of the electric negative pressure device is hermetically connected with an upper end of the waste liquid bottle through a connecting pipe.
3. The filtration device for oocytes of claim 1, wherein the filtration membrane has a filter pore size of 2 to 20 μm.
4. The oocyte filter device according to claim 1, wherein a first boss is arranged at the lower end of the specimen liquid adding pool, a first groove matched with the first boss is arranged at the upper end of the liquid adding fixing pool, and a first sealing gasket is arranged between the first boss and the first groove.
5. The oocyte filter device according to claim 1, wherein a second boss is arranged at the lower end of the liquid feeding fixing pool, a second groove matched with the second boss is arranged at the upper end of the waste liquid bottle, and a second sealing gasket is arranged between the second boss and the second groove.
6. The oocyte filter device according to claim 1, wherein a bottom plate is fastened to the bottom of the waste liquid bottle, and a plurality of through holes are formed in the bottom plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022607455.3U CN214032447U (en) | 2020-11-12 | 2020-11-12 | A filter equipment for oocyte |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022607455.3U CN214032447U (en) | 2020-11-12 | 2020-11-12 | A filter equipment for oocyte |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214032447U true CN214032447U (en) | 2021-08-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022607455.3U Active CN214032447U (en) | 2020-11-12 | 2020-11-12 | A filter equipment for oocyte |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214032447U (en) |
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2020
- 2020-11-12 CN CN202022607455.3U patent/CN214032447U/en active Active
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