CN214496311U - Nucleated cell separation detection device - Google Patents
Nucleated cell separation detection device Download PDFInfo
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- CN214496311U CN214496311U CN202022602222.4U CN202022602222U CN214496311U CN 214496311 U CN214496311 U CN 214496311U CN 202022602222 U CN202022602222 U CN 202022602222U CN 214496311 U CN214496311 U CN 214496311U
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- membrane
- microporous membrane
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- detection
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- 238000001514 detection method Methods 0.000 title claims abstract description 47
- 238000000926 separation method Methods 0.000 title claims abstract description 16
- 239000012982 microporous membrane Substances 0.000 claims abstract description 47
- 238000001914 filtration Methods 0.000 claims abstract description 39
- 239000012528 membrane Substances 0.000 claims abstract description 33
- 238000005374 membrane filtration Methods 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000003463 adsorbent Substances 0.000 claims abstract description 5
- 239000002699 waste material Substances 0.000 claims abstract description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 24
- 210000005259 peripheral blood Anatomy 0.000 abstract description 10
- 239000011886 peripheral blood Substances 0.000 abstract description 10
- 210000005266 circulating tumour cell Anatomy 0.000 abstract description 9
- 210000004881 tumor cell Anatomy 0.000 abstract description 7
- 210000004369 blood Anatomy 0.000 abstract description 6
- 239000008280 blood Substances 0.000 abstract description 6
- 210000000601 blood cell Anatomy 0.000 abstract description 4
- 239000012466 permeate Substances 0.000 abstract 1
- 238000010998 test method Methods 0.000 abstract 1
- 239000006285 cell suspension Substances 0.000 description 5
- 239000002504 physiological saline solution Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 210000003593 megakaryocyte Anatomy 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a nucleated cell separation detection device, wherein a detection unit consists of a power pump, a centrifugal machine and a filter, a rotary pump tube is arranged on the power pump, a membrane filtration interception unit consists of a specimen collection barrel, a membrane fixing unit, a pump link tube and a waste liquid barrel, and the pump link tube is connected with the power pump through the rotary pump tube; the microporous membrane fixed unit is by filtering the microporous membrane, membrane casing and identification code, microporous membrane fixed unit sets up on detection carrier mechanism, detection carrier mechanism is used for holding the filtration microporous membrane, detection carrier mechanism cooperatees with the detecting element, permeate the adsorbent solution in the filtration microporous membrane, through microporous membrane filter equipment, combine to detect can be fast with the CTCs in the peripheral blood from normal blood cell separation and enrichment, normal cell and tumor cell are distinguished to the physical characteristics of cell, can detect the CTC cell in 10ml blood, variable uniformity among the test procedure has been guaranteed, standardized processing more, only need 12min to interpret.
Description
Technical Field
The utility model relates to a biotechnology field especially involves a nucleated cell separation detection device.
Background
At present, the existing circulating tumor cells have great harmfulness, are important reasons for recurrence and distant metastasis of malignant tumors and are important reasons for death of tumor patients, in recent years, along with the development of detection technology, a filtration method becomes the currently used technology, cells are separated through a physical method, but in the prior art, a cell filtration device needs to be disassembled, assembled and rotated, a multiple filtration membrane causes influence, in addition, detection shows different coloring according to various cell components, and the detection needs a long time and is not simple, convenient and accurate enough.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide a through using millipore filtration membrane filter equipment, combine check out test set can be fast with the nucleated cell separation detection device of CTCs separation and enrichment in the peripheral blood from normal blood cell.
In order to solve the technical problem, the utility model provides a nucleated cell separation detection device, it comprises detecting element, membrane filtration interception unit and the fixed unit of microporous membrane, detecting element comprises power pump, centrifuge and filter, be equipped with the rotary type pump line on the power pump, membrane filtration interception unit comprises sample collection bucket, the fixed unit of membrane, pump chain connecting pipe and waste liquid bucket, the pump chain connecting pipe is connected with the power pump through rotary type pump line;
The microporous membrane fixing unit is composed of a filtering microporous membrane, a membrane shell and an identification code, the microporous membrane fixing unit is arranged on a detection carrier mechanism, the detection carrier mechanism is used for accommodating the filtering microporous membrane, the detection carrier mechanism is matched with the detection unit, and the filtering microporous membrane penetrates through an adsorbent solution.
As a further improvement of the utility model, still be equipped with the display part between filtration microporous membrane and the membrane casing, be equipped with the tongue edgewise on the display part.
As a further improvement of the utility model, a filter membrane bearing structure layer is arranged between the membrane shell and the display part.
As a further improvement of the utility model, the filtration microporous membrane comprises a filtration membrane with the aperture of 5-7 μm.
As a further improvement of the utility model, the filtering microporous membrane is a micron-grade soft annular membrane, and a fluorescent antibody reaction layer and a saline solution layer are arranged in the filtering microporous membrane.
The utility model adopts the above structure after, its beneficial effect does:
the utility model provides a nucleated cell separation detection device, it is held back the unit by detecting element, membrane filtration and is constituteed with the fixed unit of microporous membrane, the detecting element comprises power pump, centrifuge and filter, be equipped with the rotary type pump line on the power pump, the membrane filtration is held back the unit and is gathered bucket, membrane fixed unit, pump chain connecting pipe and waste liquid bucket by the sample and constitute, pump chain connecting pipe is connected with the power pump through rotary type pump line; the microporous membrane fixing unit comprises a filtering microporous membrane, a membrane shell and an identification code, the microporous membrane fixing unit is arranged on a detection carrier mechanism, the detection carrier mechanism is used for accommodating the filtering microporous membrane, the detection carrier mechanism is matched with the detection unit, blood is collected in the filtering microporous membrane through an adsorbent solution and a sample collecting barrel, 10ml of peripheral blood is conveyed to the detection unit through a pump linking tube, a centrifugal machine is used for centrifuging to remove blood plasma, serum contains a large number of nucleated cells, 18-22ml of physiological saline is washed for 2-3 times, the filtering microporous membrane is a micron-sized soft annular membrane and is washed in a reaction tank based on a micron-sized flexible membrane, the micronucleus cells are centrifuged by centrifugation, the detection carrier mechanism is used for accommodating the filtering microporous membrane, cell suspension contains the micronucleus cells and tumor cells, an antibody with fluorescence is added, and redundant antibodies are washed by the physiological saline, microscopic examination is carried out in cell suspension liquid, microscopic examination is carried out by a scanning fluorescence microscope, tumor cells are carried out with fluorescence, normal cells are carried out without fluorescence, the CTCs in peripheral blood can be quickly separated and enriched from the normal blood cells by combining advanced mechanical detection through a high polymer material microporous filter membrane filtering device, the normal cells and the tumor cells are distinguished by the physical characteristics of the cells, the CTC cells can be detected from 10ml of blood because the sizes of the common cells in the blood are smaller than 15 micrometers, the variable consistency in the testing process is ensured, more standardized treatment is carried out, and in addition, the application can be interpreted only within 12 min.
Drawings
Fig. 1 is a schematic structural view of the nucleated cell separation detection device of the present invention.
Detailed Description
The following detailed description of the present invention, taken in conjunction with the accompanying drawings and detailed description of preferred embodiments, is not to be construed as limiting the invention;
as shown in fig. 1, a nucleated cell separation detection device comprises a detection unit 1, a membrane filtration and interception unit 2 and a microporous membrane fixing unit 3, wherein the detection unit 1 comprises a power pump 101, a centrifuge 102 and a filter 103, a rotary pump tube 104 is arranged on the power pump 101, the membrane filtration and interception unit 2 comprises a specimen collection barrel 201, a membrane fixing unit 202, a pump link tube 203 and a waste liquid barrel 204, and the pump link tube 203 is connected with the power pump 101 through the rotary pump tube 104;
the microporous membrane fixing unit 3 is composed of a filtering microporous membrane 301, a membrane shell 302 and an identification code 303, the microporous membrane fixing unit 3 is arranged on a detection carrier mechanism 4, the detection carrier mechanism 4 is used for accommodating the filtering microporous membrane 301, the detection carrier mechanism 4 is matched with the detection unit 1, and an adsorbent solution penetrates through the filtering microporous membrane 301.
A display part 304 is also arranged between the filtering microporous membrane 301 and the membrane shell 302, and a convex groove edge 305 is arranged on the display part 304.
A filter membrane bearing structure layer 305 is arranged between the membrane shell 302 and the display part 304; the filtering microporous membrane 301 comprises a filtering membrane with the pore diameter of 5-7 mu m; the filtering microporous membrane 301 is a micron-sized soft annular membrane, and a fluorescent antibody reaction layer and a saline solution layer are arranged in the filtering microporous membrane 301.
Firstly, collecting human peripheral blood, loading peripheral blood in a sample collection barrel 201, installing a filtering microporous membrane 301 on a membrane fixing unit 202, opening a pump linking tube 203, conveying the peripheral blood to 10ml of peripheral blood to a detection unit 1 through a rotary pump tube 104, centrifuging by a centrifuge 102 to remove plasma, washing 18-22ml of physiological saline for 2-3 times, washing the filtering microporous membrane by a micron-sized flexible membrane in a reaction tank based on the micron-sized flexible membrane at a centrifugal speed of 1500r/min for 2-3 times, centrifuging by a micron-sized pore of 5 microns (3 +/-3 microns) to centrifuge off micronucleus cells, and centrifuging in the peripheral blood: tumor >30 μm, detection carrier means for housing a filter microporous membrane, the cell suspension containing megakaryocytes and tumor cells, adding fluorescent antibody and washing off excess antibody with 2-3 secondary saline to produce 0.5ml of cell suspension, performing microscopic examination in 100 μm cell suspension, scanning fluorescence microscopy, tumor cells with fluorescence, and normal cells without fluorescence.
CTCs in peripheral blood can be rapidly separated and enriched from normal blood cells by combining advanced mechanical detection, normal cells and tumor cells can be distinguished by the physical characteristics of the cells, and CTC cells can be detected from 10ml of blood because the sizes of common cells in the blood are smaller than 15 micrometers, so that the variable consistency in the testing process is ensured, the CTC cells are processed more in a standardized manner, and in addition, the CTCs can be interpreted only within 12 min.
Claims (5)
1. A nucleated cell separation detection device is characterized in that: the device is composed of a detection unit (1), a membrane filtration interception unit (2) and a microporous membrane fixing unit (3), wherein the detection unit (1) is composed of a power pump (101), a centrifuge (102) and a filter (103), a rotary pump tube (104) is arranged on the power pump (101), the membrane filtration interception unit (2) is composed of a specimen collection barrel (201), a membrane fixing unit (202), a pump linking tube (203) and a waste liquid barrel (204), and the pump linking tube (203) is connected with the power pump (101) through the rotary pump tube (104);
the microporous membrane fixing unit (3) is composed of a filtering microporous membrane (301), a membrane shell (302) and an identification code (303), the microporous membrane fixing unit (3) is arranged on a detection carrier mechanism (4), the detection carrier mechanism (4) is used for containing the filtering microporous membrane (301), the detection carrier mechanism (4) is matched with the detection unit (1), and an adsorbent solution penetrates through the filtering microporous membrane (301).
2. The nucleated cell separation detection apparatus according to claim 1, wherein: a display part (304) is further arranged between the filtering microporous membrane (301) and the membrane shell (302), and a convex groove edge (305) is arranged on the display part (304).
3. The nucleated cell separation detection apparatus according to claim 1, wherein: a filter membrane supporting structure layer (306) is arranged between the membrane shell (302) and the display part (304).
4. The nucleated cell separation detection apparatus according to claim 1, wherein: the filtering microporous membrane (301) comprises a filtering membrane with the pore diameter of 5-7 mu m.
5. The nucleated cell separation detection apparatus according to claim 1, wherein: the filtering microporous membrane (301) is a micron-sized soft annular membrane, and a fluorescent antibody reaction layer and a saline water dissolving layer are arranged in the filtering microporous membrane (301).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022602222.4U CN214496311U (en) | 2020-11-12 | 2020-11-12 | Nucleated cell separation detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022602222.4U CN214496311U (en) | 2020-11-12 | 2020-11-12 | Nucleated cell separation detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214496311U true CN214496311U (en) | 2021-10-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022602222.4U Active CN214496311U (en) | 2020-11-12 | 2020-11-12 | Nucleated cell separation detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214496311U (en) |
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2020
- 2020-11-12 CN CN202022602222.4U patent/CN214496311U/en active Active
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Effective date of registration: 20231118 Address after: 225300 West of the third to fourth floors, No. 60, Building G54, east of Lujia Road, west of Tai Road, China Medical Chengkou, Taizhou City, Jiangsu Province Patentee after: JIANGSU ANTAE BIOTECHNOLOGY Co.,Ltd. Address before: 225300 west of No. 60, building g54, west of Chengkou Tai Road and east of Lujia Road, Taizhou City, Jiangsu Province Patentee before: Taizhou Jiuxing medical laboratory Co.,Ltd. |