CN215757403U - Cell culture device - Google Patents

Cell culture device Download PDF

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Publication number
CN215757403U
CN215757403U CN202121687931.5U CN202121687931U CN215757403U CN 215757403 U CN215757403 U CN 215757403U CN 202121687931 U CN202121687931 U CN 202121687931U CN 215757403 U CN215757403 U CN 215757403U
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China
Prior art keywords
culture
culture solution
branch pipes
pipe
cell culture
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CN202121687931.5U
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Chinese (zh)
Inventor
吴亚剑
戴王祥
丁楠
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Western Medical Science And Technology Group Co ltd
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Western Medical Science And Technology Group Co ltd
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Abstract

The utility model belongs to the technical field of cell culture, and particularly relates to a cell culture device which comprises a base, wherein culture vessels are arranged on the top of the base at equal intervals, air cylinders are arranged on the periphery of the top of the base, mounting plates are arranged on the tops of the four air cylinders, and a plurality of sealing covers are arranged on the bottom wall of each mounting plate. When the utility model needs to provide the culture solution, the control valve on the output pipe of the oxygen generator is firstly ensured to be closed, then the pump is started to send the liquid in the culture solution box into the branch pipe through the main pipe, and the liquid is uniformly sprayed in the culture vessel through the holes of the branch pipes, thereby replacing manual spraying and simultaneously improving the uniformity of spraying; when oxygen is needed to be supplied, the control valve on the output pipe of the pump is firstly ensured to be closed, then the oxygen generator is started to send the oxygen into the branch pipe through the main pipe, and the oxygen is sent into the culture vessel through the holes of the multiple branch pipes, so that the oxygen content in the culture vessel is greatly improved, and the culture vessel is helpful for culture.

Description

Cell culture device
Technical Field
The utility model relates to the technical field of cell culture, in particular to a cell culture device.
Background
Cell culture devices are used for the cultivation of cells and typically comprise a cell culture vessel. Typically, cell culture vessels include culture flasks, culture dishes, and culture plates. The main materials of the cell culture vessel are basically divided into two types, plastic and glass. The glass cell culture container can be used for culturing plant materials and microorganisms, and can also be used for adherent culture of animal cells; the plastic cell culture container may be of polyethylene material, disposable and reusable.
When cultivateing partial cell, sometimes need the manual work to spray the culture solution, improve the culture effect, and the mode of ordinary manual work spraying culture solution not only consumes the manpower, and the degree of consistency of spraying is also relatively poor to, partial cell needs better oxygen content at the in-process of cultivateing, just can help cultivating, consequently needs to develop a cell culture device.
SUMMERY OF THE UTILITY MODEL
This section is for the purpose of summarizing some aspects of embodiments of the utility model and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the abstract of the specification and the title of the application to avoid obscuring the purpose of this section, the abstract of the specification and the title of the application, and such simplifications or omissions are not intended to limit the scope of the utility model.
The present invention has been made in view of the problems occurring in the prior art.
Therefore, the utility model aims to provide a cell culture device, when culture solution needs to be provided, after a control valve on an output pipe of an oxygen generator is firstly ensured to be closed, a pump machine is started to send liquid in a culture solution box into branch pipes through a main pipe, and the liquid is uniformly sprayed in a culture vessel through holes of a plurality of branch pipes, so that the manual spraying is replaced, and the spraying uniformity is improved; when oxygen needs to be supplied, the control valve on the output pipe of the pump is firstly ensured to be closed, then the oxygen generator is started to send the oxygen into the branch pipes through the main pipe, and the oxygen is sent into the culture vessel through the holes of the plurality of branch pipes, so that the oxygen content in the culture vessel is greatly improved, and the culture vessel is beneficial to culture; when the culture vessel needs to be sealed and stored, the mounting plate is lowered by starting the four cylinders, and the sealing cover can seal and store the culture vessel. This device function is comprehensive, and it is convenient to use, facilitate promotion.
To solve the above technical problem, according to an aspect of the present invention, the present invention provides the following technical solutions:
a cell culture apparatus, comprising:
the device comprises a base, wherein culture vessels are arranged on the top of the base at equal intervals, air cylinders are arranged on the periphery of the top of the base, mounting plates are arranged on the tops of the four air cylinders, a plurality of sealing covers are arranged on the bottom wall of each mounting plate, and the plurality of sealing covers are matched with the plurality of culture vessels below the sealing covers;
the oxygen generator is arranged close to the left of the top of the mounting plate, a main pipeline is arranged on an output pipe of the oxygen generator, a plurality of branch pipelines are arranged at the bottom of the main pipeline at equal intervals, the branch pipelines are communicated with the main pipeline, the bottom ends of the branch pipelines penetrate through the sealing covers respectively and are positioned above the culture vessel, a plurality of branch pipes are uniformly distributed on the periphery of the outer side wall of the bottom end of the branch pipeline, the branch pipes are communicated with the branch pipelines, and a plurality of holes are formed in the bottom of each branch pipe;
the culture solution case, the mounting panel top is provided with by the right side the culture solution case, culture solution case left side is provided with the pump machine, the input tube intercommunication of pump machine culture solution incasement chamber bottom, the output tube connection of pump machine the right-hand member of trunk line.
As a preferable aspect of a cell culture apparatus according to the present invention, wherein: every sealing cover bottom all is provided with the sealing washer, the base bottom is provided with the slipmat.
As a preferable aspect of a cell culture apparatus according to the present invention, wherein: the output pipe of the oxygen generator and the output pipe of the culture solution box are both provided with control valves.
As a preferable aspect of a cell culture apparatus according to the present invention, wherein: the lateral wall is provided with the visual window before the culture solution case, visual window surface is provided with the liquid level scale.
As a preferable aspect of a cell culture apparatus according to the present invention, wherein: the culture solution box top has seted up the water filling port, the water filling port top is provided with the top cap.
Compared with the prior art, the utility model has the beneficial effects that: when the culture solution needs to be provided, the control valve on the output pipe of the oxygen generator is firstly ensured to be closed, then the pump machine is started to send the liquid in the culture solution box into the branch pipes through the main pipe, and the liquid is uniformly sprayed into the culture vessel through the holes of the branch pipes, so that the manual spraying is replaced, and the spraying uniformity is improved; when oxygen needs to be supplied, the control valve on the output pipe of the pump is firstly ensured to be closed, then the oxygen generator is started to send the oxygen into the branch pipes through the main pipe, and the oxygen is sent into the culture vessel through the holes of the plurality of branch pipes, so that the oxygen content in the culture vessel is greatly improved, and the culture vessel is beneficial to culture; when the culture vessel needs to be sealed and stored, the mounting plate is lowered by starting the four cylinders, and the sealing cover can seal and store the culture vessel. This device function is comprehensive, and it is convenient to use, facilitate promotion.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail with reference to the accompanying drawings and detailed embodiments, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise. Wherein:
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a schematic bottom view of the sealing cap of the present invention;
FIG. 3 is a schematic top view of the base of the present invention;
FIG. 4 is a schematic structural view of the sealing lid of the present invention sealing a culture vessel;
in the figure: the culture container comprises a base 100, a culture vessel 110, a cylinder 120, a mounting plate 130, a sealing cover 140, a sealing ring 150, a non-slip mat 160, an oxygen generator 200, a main pipeline 210, a branch pipeline 220, a branch pipe 230, a hole 240, a culture solution tank 300, a visible window 310, a water injection port 320 and a pump 330.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described herein, and it will be apparent to those of ordinary skill in the art that the present invention may be practiced without departing from the spirit and scope of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Next, the present invention will be described in detail with reference to the drawings, and in the detailed description of the embodiments of the present invention, the cross-sectional views illustrating the structure of the device are not enlarged partially according to the general scale for convenience of illustration, and the drawings are only examples, which should not limit the scope of the present invention. In addition, the three-dimensional dimensions of length, width and depth should be included in the actual fabrication.
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail with reference to the accompanying drawings.
Fig. 1 to 4 are schematic structural views illustrating an embodiment of a cell culture apparatus according to the present invention, and referring to fig. 1 to 4, the cell culture apparatus according to the embodiment includes:
the culture utensil assembly comprises a base 100, wherein culture utensils 110 are arranged on the top of the base 100 at equal intervals, air cylinders 120 are arranged on the periphery of the top of the base 100, mounting plates 130 are arranged on the tops of the four air cylinders 120, a plurality of sealing covers 140 are arranged on the bottom wall of the mounting plate 130, the sealing covers 140 are matched with the culture utensils 110 below the sealing covers 140, a sealing ring 150 is arranged at the bottom of each sealing cover 140, the sealing performance between each sealing cover 140 and the culture utensil 110 is improved by the sealing ring 150, a non-slip mat 160 is arranged at the bottom of the base 100, and the stability of the assembly when the assembly is placed integrally is improved by the non-slip mat 160;
the oxygen generator 200 is arranged on the left of the top of the mounting plate 130, the oxygen generator 200 is arranged on the output pipe of the oxygen generator 200, a plurality of branch pipelines 220 are arranged at the bottom of the main pipeline 210 at equal intervals, the branch pipelines 220 are communicated with the main pipeline 210, the bottom ends of the branch pipelines 220 penetrate through the sealing covers 140 respectively and are located above the culture vessel 110, a plurality of branch pipes 230 are uniformly distributed on the periphery of the outer side wall of the bottom end of the branch pipeline 220, the branch pipes 230 are communicated with the branch pipelines 220, and a plurality of holes 240 are formed in the bottom of each branch pipe 230;
culture solution case 300, mounting panel 130 top is provided with culture solution case 300 by the right side, culture solution case 300 left side is provided with pump machine 330, the input tube intercommunication culture solution case 300 inner chamber bottom of pump machine 330, the right-hand member of output tube connection trunk line 210 of pump machine 330, oxygenerator 200's the output tube and culture solution case 300's output tube all are provided with the control valve, lateral wall is provided with visual window 310 before culture solution case 300, visual window 310 surface is provided with the liquid level scale, conveniently follow the inside surplus liquid content of outside observation culture solution case 300, water filling port 320 has been seted up at culture solution case 300 top, water filling port 320 top is provided with the top cap, water filling port 320 is used for the culture solution case 300 inner chamber injection culture solution.
In the specific using process, when the culture solution is required to be provided in the using process of the utility model, after the control valve on the output pipe of the oxygen generator 200 is firstly ensured to be closed, the pump 330 is started to send the liquid in the culture solution tank 300 into the branch pipes 220 through the main pipe 210, and the liquid is uniformly sprayed into the culture vessel 110 through the holes 240 of the plurality of branch pipes 230, so that the spraying uniformity is improved while manual spraying is replaced;
when oxygen needs to be supplied, the control valve on the output pipe of the pump 330 is firstly ensured to be closed, then the oxygen generator 200 is started to send the oxygen into the branch pipe 220 through the main pipe 210, and the oxygen is sent into the culture vessel through the holes 240 of the plurality of branch pipes 230, so that the oxygen content in the culture vessel 110 is greatly improved, and the culture is facilitated;
when the culture vessels 110 need to be subjected to cover preservation, the four air cylinders 120 are started, the mounting plate 130 is lowered, and the sealing covers 140 can be used for performing cover preservation on the culture vessels 110. This device function is comprehensive, and it is convenient to use, facilitate promotion.
While the utility model has been described above with reference to an embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the utility model. In particular, the various features of the disclosed embodiments of the utility model may be used in any combination, provided that no structural conflict exists, and the combinations are not exhaustively described in this specification merely for the sake of brevity and resource conservation. Therefore, it is intended that the utility model not be limited to the particular embodiments disclosed, but that the utility model will include all embodiments falling within the scope of the appended claims.

Claims (5)

1. A cell culture apparatus, comprising:
the culture device comprises a base (100), wherein culture vessels (110) are arranged on the top of the base (100) at equal intervals, air cylinders (120) are arranged on the periphery of the top of the base (100), mounting plates (130) are arranged on the tops of the four air cylinders (120), a plurality of sealing covers (140) are arranged on the bottom wall of each mounting plate (130), and the plurality of sealing covers (140) are matched with the plurality of culture vessels (110) below the sealing covers;
the top of the mounting plate (130) is provided with the oxygen generator (200) close to the left, an output pipe of the oxygen generator (200) is provided with a main pipe (210), a plurality of branch pipes (220) are arranged at the bottom of the main pipe (210) at equal intervals, the branch pipes (220) are communicated with the main pipe (210), the bottom ends of the branch pipes (220) penetrate through the sealing covers (140) respectively and are located above the culture vessel (110), a plurality of branch pipes (230) are uniformly distributed around the outer side wall of the bottom end of the branch pipe (220), the branch pipes (230) are communicated with the branch pipes (220), and a plurality of holes (240) are formed in the bottom of each branch pipe (230);
culture solution box (300), mounting panel (130) top is provided with by the right side culture solution box (300), culture solution box (300) left side is provided with pump machine (330), the input tube intercommunication of pump machine (330) culture solution box (300) inner chamber bottom, the output tube of pump machine (330) is connected the right-hand member of trunk line (210).
2. A cell culture apparatus according to claim 1, wherein: each sealing cover (140) bottom all is provided with sealing washer (150), base (100) bottom is provided with slipmat (160).
3. A cell culture apparatus according to claim 1, wherein: the output pipe of the oxygen generator (200) and the output pipe of the culture solution box (300) are both provided with control valves.
4. A cell culture apparatus according to claim 1, wherein: the side wall is provided with visual window (310) before culture solution case (300), visual window (310) surface is provided with the liquid level scale.
5. A cell culture apparatus according to claim 1, wherein: the top of the culture solution box (300) is provided with a water injection port (320), and the top of the water injection port (320) is provided with a top cover.
CN202121687931.5U 2021-07-23 2021-07-23 Cell culture device Active CN215757403U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202121687931.5U CN215757403U (en) 2021-07-23 2021-07-23 Cell culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202121687931.5U CN215757403U (en) 2021-07-23 2021-07-23 Cell culture device

Publications (1)

Publication Number Publication Date
CN215757403U true CN215757403U (en) 2022-02-08

Family

ID=80106959

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202121687931.5U Active CN215757403U (en) 2021-07-23 2021-07-23 Cell culture device

Country Status (1)

Country Link
CN (1) CN215757403U (en)

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