CN215569766U - Series-parallel pipeline for cell factory connection - Google Patents
Series-parallel pipeline for cell factory connection Download PDFInfo
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- CN215569766U CN215569766U CN202121330816.2U CN202121330816U CN215569766U CN 215569766 U CN215569766 U CN 215569766U CN 202121330816 U CN202121330816 U CN 202121330816U CN 215569766 U CN215569766 U CN 215569766U
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Abstract
The utility model discloses a series-parallel connection pipeline for connecting cell factories, which comprises a first T-shaped connection pipeline, a second T-shaped connection pipeline and a third T-shaped connection pipeline, wherein one end of the first T-shaped connection pipeline is connected with a transfection medium, the other two ends of the first T-shaped connection pipeline are respectively connected with one end of the second T-shaped connection pipeline and one end of the third T-shaped connection pipeline, the other end of the second T-shaped connection pipeline is connected with a first cell factory, the other end of the second T-shaped connection pipeline is connected with a first waste liquid bag, the other end of the third T-shaped connection pipeline is connected with a second cell factory, the other end of the third T-shaped connection pipeline is connected with a second waste liquid bag, the second T-shaped connection pipeline is connected with a first peristaltic pump, the third T-shaped connection pipeline is connected with a second peristaltic pump, the simultaneous waste discharge, the simultaneous transfection and the liquid change operation of the culture liquids of the two cell factories are realized, the operation time is greatly saved, The labor hour and the like of personnel can quickly meet the requirement of process operation time limit, and the device has a simple structure and is convenient to use.
Description
Technical Field
The utility model relates to the field of series-parallel pipelines connected in a cell factory, in particular to a series-parallel pipeline for connecting the cell factory.
Background
The slow virus carrier adherence production process usually relates to plasmid transfection and liquid exchange operation of a cell factory, the traditional operation mode adopts an opening in a biological safety cabinet or adopts a single pipeline to carry out transfection or liquid exchange operation, the operation process is time-consuming, the efficiency is low, the uniformity and the stability among batches are poor, the operation time limit is shortened for improving the efficiency of production operation so as to better meet the production requirement of the slow virus carrier, and the pipeline design is designed.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem of providing a series-parallel pipeline for connecting cell factories, wherein a first T-shaped connecting pipeline, a second T-shaped connecting pipeline and a third T-shaped connecting pipeline are connected in series and are connected with a first peristaltic pump and a second peristaltic pump to communicate a transfection medium, the cell factories and a waste liquid bag, so that the simultaneous waste discharge, the simultaneous transfection and liquid change of culture liquids of the two cell factories are realized, the operation time, the labor hour and the like are greatly saved, the process operation time limit requirement is quickly met, the structure is simple, the use is convenient, and the defects caused by the prior art are overcome.
In order to solve the technical problems, the utility model provides the following technical scheme:
The utility model provides a series-parallel connection pipeline for cell factory connects, wherein, contains first T type connecting line, second T type connecting line, third T type connecting line, the one end of first T type connecting line is connected with the transfection culture medium, both ends in addition respectively with second T type connecting line one end third T type connecting line's one end is connected, first cell factory is connected to the other end of second T type connecting line, first waste liquid bag is connected to the other end, second cell factory is connected to the other end of third T type connecting line, the second waste liquid bag is connected to the other end, second T type connecting line connection be connected with first peristaltic pump on the pipeline of first cell factory, third T type connecting line connection be connected with the second peristaltic pump on the pipeline of second cell factory, realize a series-parallel connection pipeline connection piping arrangement, the supplementary setting first peristaltic pump, The second peristaltic pump can realize the purpose of simultaneously discharging two cell factories and transfecting the two cell factories without switching the position of the peristaltic pump, thereby greatly saving the transfection time of the cell factories.
The series-parallel connection pipeline for connecting the cell factory is characterized in that the first T-shaped connecting pipeline is connected with the second T-shaped connecting pipeline, the third T-shaped connecting pipeline and the transfection medium through sterile welding;
The second T-shaped connecting pipeline is connected with the first cell factory and the first waste liquid bag through aseptic welding;
and the third T-shaped connecting pipeline is connected with the second waste liquid bag through aseptic welding.
The series-parallel pipeline for connection of the cell factory further comprises two scales, and the first cell factory and the second cell factory are respectively placed on the tops of the two scales.
Foretell a series-parallel pipeline for cell factory connects, wherein, first T type connecting line second T type connecting line third T type connecting line comprises T type reducing joint, silica gel pump line, thermoplasticity pipe, the three tip that T type reducing connects all is connected with the silica gel pump line, the other end of silica gel pump line all is connected with the thermoplasticity pipe, through gamma irradiation sterilization or adopt the processing mode of damp and hot sterilization and use in the technology production as aseptic material consumptive material.
The series-parallel pipeline for cell factory connection is characterized in that the inner diameter of the silica gel pump tube is 1/4 inches, the outer diameter of the silica gel pump tube is 7/16 inches, the silica gel pump tube can be matched with the peristaltic pump, and long-time liquid transfer, reverse waste discharge and the like are supported under the condition that the position of the peristaltic pump and the position of the pipeline are not switched.
The series-parallel pipeline for connection of the cell factory is characterized in that the inner diameter of the thermoplastic pipe is 1/8 inches, the outer diameter of the thermoplastic pipe is 1/4 inches, and the thermoplastic pipe is welded with the corresponding cell factory or waste liquid bag in a sterile mode, so that the sterility is guaranteed.
Foretell a series-parallel connection pipeline for cell factory connects can use in the transfection of viral vector adherent culture technology, operation steps such as liquid changing, and each tube coupling mode is aseptic tube connector welding, has avoided open operating risk, simultaneously through fixed first T type connecting tube second T type connecting tube third T type connecting tube with first peristaltic pump the setting of second peristaltic pump can realize first cell factory two cell factory's of second cell factory culture solution is wasted discharge simultaneously and is transfected simultaneously, the liquid changing operation, save operating time, personnel man-hour greatly etc. satisfy technology operation time limit demand fast.
When the cell factory is used, the first cell factory and the second cell factory in the series-parallel pipelines for cell factory connection are placed on a peeled balance, the pipelines leading to the first T-shaped connecting pipeline are clamped, the first peristaltic pump and the second peristaltic pump are adjusted to discharge waste liquid, after the operation is finished, the second T-shaped connecting pipeline and the third T-shaped connecting pipeline are respectively clamped to the pipelines leading to the first waste liquid bag and the second waste liquid bag, the transfection culture medium bag is opened to the pipelines of the first cell factory and the second cell factory, the first peristaltic pump and the second peristaltic pump are respectively arranged and started to carry out transfection liquid inlet, after the liquid inlet is finished, the first peristaltic pump and the second peristaltic pump are stopped, the operation is finished, another group of cell factories is switched, and the operation is repeated, wherein the first waste liquid bag is clamped and the second peristaltic pump are respectively started to carry out transfection liquid inlet, after the operation is finished, the first peristaltic pump and the second peristaltic pump are stopped, the operation is finished, and the other group of cell factories is switched, and the operation is repeated The second waste bag can be reused, and 4h after transfection is finished, liquid is changed (another culture medium, a culture medium suitable for virus packaging) and the operation is similar to the transfection step.
The technical scheme provided by the series-parallel pipeline for cell factory connection has the following technical effects:
adopt first T type connecting tube, second T type connecting tube, third T type connecting tube to carry out series connection and be connected with first peristaltic pump, second peristaltic pump and will transfect culture medium, cell factory, waste liquid bag intercommunication, realize that the culture solution of two cell factories simultaneously wastes discharge and transfect simultaneously, trade liquid operation, save operating time, personnel man-hour etc. greatly, satisfy technology operation time limit demand fast, simple structure, convenient to use.
Drawings
FIG. 1 is a schematic diagram of a series-parallel connection circuit for cell factory connection according to the present invention.
Wherein the reference numbers are as follows:
a first T-shaped connecting pipeline 101, a second T-shaped connecting pipeline 102, a third T-shaped connecting pipeline 103, a transfection medium 104, a first cell factory 105, a first waste liquid bag 106, a second cell factory 107, a second waste liquid bag 108, a first peristaltic pump 109, a second peristaltic pump 110 and a balance 111.
Detailed Description
In order to make the technical means, the inventive features, the objectives and the functions of the present invention easy to understand, the present invention will be further described with reference to the following detailed drawings.
The utility model provides a series-parallel pipeline for connecting cell factories, and aims to connect a transfection medium, a cell factory and a waste liquid bag in series by adopting a first T-shaped connecting pipeline, a second T-shaped connecting pipeline and a third T-shaped connecting pipeline and connecting a first peristaltic pump and a second peristaltic pump to communicate the transfection medium, the cell factory and the waste liquid bag, so that the simultaneous waste discharge, the simultaneous transfection and liquid change of culture liquids of two cell factories are realized, the operation time, the labor hour and the like are greatly saved, the process operation time limit requirement is quickly met, the structure is simple, and the use is convenient.
As shown in FIG. 1, a series-parallel connection pipeline for cell factory connection comprises a first T-shaped connection pipeline 101, a second T-shaped connection pipeline 102, and a third T-shaped connection pipeline 103, wherein one end of the first T-shaped connection pipeline 101 is connected with a transfection medium 104, the other two ends are respectively connected with one end of the second T-shaped connection pipeline 102 and one end of the third T-shaped connection pipeline 103, the other end of the second T-shaped connection pipeline 102 is connected with a first cell factory 105, the other end is connected with a first waste liquid bag 106, the other end of the third T-shaped connection pipeline is connected with a second cell factory 107, the other end is connected with a second waste liquid bag 108, the second T-shaped connection pipeline 102 is connected with a first peristaltic pump 109 on the pipeline of the first cell factory 105, the third T-shaped connection pipeline 103 is connected with a second peristaltic pump 110 on the pipeline of the second cell factory 107, thereby realizing a series-parallel connection pipeline device, the first peristaltic pump 109 and the second peristaltic pump 110 which are arranged in an auxiliary manner can achieve the purpose of simultaneously discharging two cell factories and transfecting the two cell factories without switching the positions of the peristaltic pumps, thereby greatly saving the transfection time of the cell factories.
The series-parallel connection pipeline for connecting the cell factory is characterized in that the first T-shaped connecting pipeline 101, the second T-shaped connecting pipeline 102, the third T-shaped connecting pipeline 103 and the transfection medium 104 are connected through aseptic welding;
the second T-shaped connecting pipe 102 is connected to the first cell factory 105 and the first waste liquid bag 106 by aseptic welding;
the third T-shaped connecting pipeline 103 is connected with the second waste liquid bag 108 through aseptic welding.
The series-parallel circuit for cell factory connection as described above further comprises two scales 111, the first cell factory 105 and the second cell factory 107 being placed on top of the two scales 111, respectively.
The series-parallel pipeline for cell factory connection comprises a first T-shaped connecting pipeline 101, a second T-shaped connecting pipeline 102 and a third T-shaped connecting pipeline 103, wherein the first T-shaped connecting pipeline, the second T-shaped connecting pipeline and the third T-shaped connecting pipeline are all composed of T-shaped reducing joints, silica gel pump pipes and thermoplastic pipes, three joint ends of the T-shaped reducing joints are all connected with the silica gel pump pipes, the other ends of the silica gel pump pipes are all connected with the thermoplastic pipes, and the silica gel pump pipes are sterilized by gamma irradiation or by adopting a moist-heat sterilization treatment mode and are applied to process production as sterile material consumables.
The series-parallel pipeline for cell factory connection is characterized in that the inner diameter of the silica gel pump tube is 1/4 inches, the outer diameter of the silica gel pump tube is 7/16 inches, the silica gel pump tube can be matched with a peristaltic pump, and under the condition that the position of the peristaltic pump and the position of the pipeline are not switched, long-time liquid transfer, reverse direction waste discharge and the like are supported.
The series-parallel pipeline for connection of the cell factory is characterized in that the inner diameter of the thermoplastic pipe is 1/8 inches, the outer diameter of the thermoplastic pipe is 1/4 inches, and the thermoplastic pipe is in aseptic welding with the corresponding cell factory or waste liquid bag, so that the sterility is guaranteed.
The series-parallel connection pipeline for cell factory connection can be applied to the operation steps of transfection, liquid changing and the like of a virus carrier adherent culture process, all pipeline connection modes are aseptic pipe connecting machine welding, the open operation risk is avoided, meanwhile, the culture liquids of two cell factories of a first cell factory 105 and a second cell factory 107 can be simultaneously discharged, transfected and changed through the arrangement of the first T-shaped connection pipeline 101, the second T-shaped connection pipeline 102 and the third T-shaped connection pipeline 103, the first peristaltic pump 109 and the second peristaltic pump 110, the operation time is greatly saved, the labor hour is greatly reduced, and the process operation time limit requirement is quickly met.
When in use, the first cell factory 105 and the second cell factory 107 in the series-parallel pipelines for cell factory connection are placed on a peeled balance 111, the pipelines leading to the first T-shaped connecting pipeline 101 are clamped, the first peristaltic pump 109 and the second peristaltic pump 110 are adjusted to discharge waste liquid, after the waste liquid discharge, the second T-shaped connecting pipeline 102 and the third T-shaped connecting pipeline 103 are respectively clamped to the pipelines leading to the first waste liquid bag 106 and the second waste liquid bag 108, the bag of the transfection medium 104 is opened to the pipeline clamps of the first cell factory 105 and the second cell factory 107, the first peristaltic pump 109 and the second peristaltic pump 110 are respectively arranged and started to feed the transfection liquid, after the feed liquid is finished, the first peristaltic pump 109 and the second peristaltic pump 110 are stopped, the operation is finished, another group of cell factories is switched, the operation is repeated, wherein the first waste liquid bag 106 and the second waste liquid bag 108 can be reused, 4h after transfection was complete, a change of medium (another medium, a medium suitable for virus packaging) was performed, operating similarly to the transfection step.
In summary, the series-parallel connection pipeline for cell factory connection provided by the utility model adopts the first T-shaped connection pipeline, the second T-shaped connection pipeline and the third T-shaped connection pipeline which are connected in series and connected with the first peristaltic pump and the second peristaltic pump to communicate the transfection medium, the cell factory and the waste liquid bag, so that the simultaneous waste discharge, the simultaneous transfection and liquid change operation of the culture liquids of the two cell factories are realized, the operation time, the labor hour and the like are greatly saved, the process operation time limit requirement is quickly met, the structure is simple, and the use is convenient.
The above description has been made of specific embodiments of the present invention. It is to be understood that the utility model is not limited to the particular embodiments described above, and that devices and structures not described in detail are understood to be implemented in a manner common in the art; various changes or modifications may be made by those skilled in the art within the scope of the appended claims without departing from the spirit of the utility model.
Claims (6)
1. The utility model provides a series-parallel connection pipeline for cell factory connects, its characterized in that contains first T type connecting line, second T type connecting line, third T type connecting line, the one end of first T type connecting line is connected with the transfection culture medium, other both ends respectively with second T type connecting line one end third T type connecting line's one end is connected, first cell factory is connected to the other end of second T type connecting line, first waste liquid bag is connected to the other end, second cell factory, second waste liquid bag are connected to the other end of third T type connecting line, second T type connecting line connects be connected with first peristaltic pump on the pipeline of first cell factory, third T type connecting line connects be connected with the second peristaltic pump on the pipeline of second cell factory.
2. The series-parallel connection line for cell factory connection according to claim 1, wherein said first T-shaped connection line is connected to said second T-shaped connection line, said third T-shaped connection line, said transfection medium by aseptic welding;
the second T-shaped connecting pipeline is connected with the first cell factory and the first waste liquid bag through aseptic welding;
and the third T-shaped connecting pipeline is connected with the second waste liquid bag through aseptic welding.
3. A series-parallel circuit for connection of a cell factory according to claim 2, further comprising two scales, said first cell factory, said second cell factory being placed on top of said two scales, respectively.
4. The series-parallel connection pipeline for cell factory connection according to any one of claims 1-3, wherein said first T-shaped connection pipeline, said second T-shaped connection pipeline, said third T-shaped connection pipeline are all composed of T-shaped reducing joints, silica gel pump pipes, thermoplastic pipes, three joint ends of said T-shaped reducing joints are all connected with said silica gel pump pipes, and the other ends of said silica gel pump pipes are all connected with said thermoplastic pipes.
5. The series-parallel line for cell factory connection according to claim 4, wherein said silicone pump tube has an inner diameter of 1/4 inches and an outer diameter of 7/16 inches.
6. The series-parallel line for a cell factory connection according to claim 5, wherein said thermoplastic tube has an inner diameter of 1/8 inches and an outer diameter of 1/4 inches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121330816.2U CN215569766U (en) | 2021-06-16 | 2021-06-16 | Series-parallel pipeline for cell factory connection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121330816.2U CN215569766U (en) | 2021-06-16 | 2021-06-16 | Series-parallel pipeline for cell factory connection |
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| Publication Number | Publication Date |
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| CN215569766U true CN215569766U (en) | 2022-01-18 |
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| CN202121330816.2U Active CN215569766U (en) | 2021-06-16 | 2021-06-16 | Series-parallel pipeline for cell factory connection |
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- 2021-06-16 CN CN202121330816.2U patent/CN215569766U/en active Active
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