CN212610703U - Passage and cell harvesting pipeline system of cell factory - Google Patents
Passage and cell harvesting pipeline system of cell factory Download PDFInfo
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- CN212610703U CN212610703U CN202022185260.4U CN202022185260U CN212610703U CN 212610703 U CN212610703 U CN 212610703U CN 202022185260 U CN202022185260 U CN 202022185260U CN 212610703 U CN212610703 U CN 212610703U
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Abstract
The utility model provides a passage and cell harvest pipe-line system of cell factory, this pipe-line system include a main line that constitutes by multistage pipeline and a plurality of three-way switch interval are continuous, and each three-way switch on the main line passes through the pipeline and connects respectively: a plurality of cell factories to be treated, a waste liquid tank, a cleaning liquid tank, a digestion liquid tank, a termination liquid tank, a cell suspension liquid tank and a plurality of cell factories to be inoculated; wherein each tank is connected with an air filter which is conveniently connected with a pressure vacuum pump to form negative pressure or positive pressure to provide power for the liquid flow. The pipeline system of the utility model can complete a series of operations of collecting cell supernatant, washing cells, digesting pancreatin, stopping digestion, recovering cell suspension and subculturing by adjusting each three-way switch. The advantages are that: and a plurality of cell factories are processed simultaneously, so that the time is saved, and the problems of nonuniform cell digestion degree, multiple open operation times, large pollution risk and the like in the process of sequentially processing a single cell factory are solved.
Description
Technical Field
The utility model belongs to the technical field of it is biological, relate to in the aspect of the cell culture, concretely relates to passage and cell harvest pipe-line system of cell factory.
Background
In the field of biotechnology, cells may be used both as tool cells for the production of, e.g., vaccines and recombinant proteins, and as cells of interest for direct use, e.g., stem cells. Therefore, the in vitro large-scale cell culture technique is a very important technical means, and the amplification is the simplest and most common culture method by using a cell factory, which utilizes the maximum culture area in a limited space, so that the batch culture of adherent cells or suspension cells is possible. Even if a bioreactor is used for larger-scale cell culture, a cell factory is often used for cell amplification to prepare a seed solution in a seed cell preparation stage of seeding.
At present, cell factories commonly use 1 layer, 2 layers, 4 layers, 10 layers, 40 layers and other specifications, and compared with the traditional cell culture bottle culture mode, the cell factories have low pollution risk, save space and are suitable for GMP production. However, when a plurality of cell factories need to be treated simultaneously in the operation of cell factory culture, there are many problems in the sequential operation: 1. when a plurality of cell factories are processed in sequence, the operation time is long; 2. the degree of cellular digestion in each cell factory is difficult to be consistent; 3. the pipeline is an open operation when being changed from one cell factory to another cell factory, and the problems of multiple open operations, large pollution risk and the like caused by the replacement of the pipeline for multiple times are solved. Therefore, a simple and efficient pipeline system is needed to simultaneously operate a plurality of cell factories, so that the cell factories can culture cells more conveniently, quickly and safely.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a passage and cell harvest pipe-line system of cell factory conveniently handles a plurality of cell factories simultaneously to save time, and cell digestion degree is inhomogeneous when avoiding handling single cell factory in proper order, open operation number of times is many, the big scheduling problem of pollution risk.
In order to realize the utility model discloses a purpose, the utility model discloses establish a passage and cell results pipe-line system of cell factory, including a main line that links to each other the constitution by multistage pipeline and a plurality of three way switch 3 interval, each three way switch 3 on the main line is connected respectively through the pipeline: a plurality of cell factories to be treated 2, a waste liquid tank 4, a cleaning liquid tank 6, a digestion liquid tank 7, a termination liquid tank 8, a cell suspension liquid tank 9 and a plurality of cell factories to be inoculated 10; wherein, each pot is connected with an air filter 5 which is conveniently connected with a pressure vacuum pump to form negative pressure or positive pressure to provide power for the liquid flow.
Wherein, the pipeline is a nontoxic silicone tube or a plastic tube, and the length of the pipeline can be adjusted according to the production scale and the actual situation.
Wherein, the three-way switch 3 on the main pipeline is connected with the cell factory through the pipeline and the joint 1; where the adapter 1 is a PC adapter or a quick adapter that can be docked with the cell factory.
Wherein, the three-way switch 3 is T-shaped or Y-shaped, and the type and the number of the three-way switch 3 can be selected according to the production scale and the actual situation.
Wherein, the specifications and the number of the cell factories 2 to be processed and the cell factories 10 to be inoculated can be set according to the actual situation, and the cell factories can be 1 layer, 2 layers, 4 layers, 10 layers or 40 layers; the number of cell factories 2 to be treated or cell factories 10 to be seeded is 1-20.
Wherein, the waste liquid tank 4, the cleaning liquid tank 6, the digestion liquid tank 7, the termination liquid tank 8 and the cell suspension liquid tank 9 are respectively bottled or plastic liquid tanks with glass fiber openings of different specifications according to the production scale and the actual situation.
The utility model has the advantages that:
1. a plurality of cell factories are processed simultaneously, so that time is saved;
2. a plurality of cell factories are digested simultaneously, so that the nonuniform digestion degree of cells is avoided when single cell factories are treated sequentially, and the uniformity of the cell state is ensured;
3. the whole operation of cell passage or cell harvest can be finished by connecting the pipeline once, the open operation times are reduced, and the pollution risk is greatly reduced.
Drawings
FIG. 1: the utility model discloses a passage and cell harvest pipe-line system structure sketch map of cell factory.
The labels in the figure are: 1: a joint; 2: a plant of cells to be treated; 3: a three-way switch; 4: a waste liquid tank; 5: an air filter; 6: cleaning a liquid tank; 7: a digestion liquid tank; 8: terminating the liquid tank; 9: a cell suspension tank; 10: the cell factory is to be seeded.
Detailed Description
To illustrate the present invention more clearly, the following embodiments are described in detail with reference to the accompanying drawings, and it should be understood that these embodiments are merely illustrative of the present invention and do not limit the scope of the present invention.
Example 1: passage and cell harvesting pipeline system of cell factory
As shown in fig. 1, a passage and cell harvesting pipeline system of a cell factory comprises a main pipeline formed by connecting a plurality of sections of pipelines and a plurality of three-way switches 3 at intervals, wherein each three-way switch 3 on the main pipeline is respectively connected through a pipeline: a plurality of cell factories to be treated 2, a waste liquid tank 4, a cleaning liquid tank 6, a digestion liquid tank 7, a termination liquid tank 8, a cell suspension liquid tank 9 and a plurality of cell factories to be inoculated 10; wherein, each pot is connected with an air filter 5 which is conveniently connected with a pressure vacuum pump to form negative pressure or positive pressure to provide power for the liquid flow. The three-way switch on the main pipeline is connected with the cell factory through the pipeline and the joint 1.
In this embodiment, the pipeline is a plastic pipe; the joint is a quick joint; each jar is a glass fiber opening split-charging bottle; the tee joint is a Y-shaped tee joint.
Example 2: passage and cell harvesting pipeline system of cell factory
As shown in fig. 1, a passage and cell harvesting pipeline system of a cell factory comprises a main pipeline formed by connecting a plurality of sections of pipelines and a plurality of three-way switches 3 at intervals, wherein each three-way switch 3 on the main pipeline is respectively connected through a pipeline: a plurality of cell factories to be treated 2, a waste liquid tank 4, a cleaning liquid tank 6, a digestion liquid tank 7, a termination liquid tank 8, a cell suspension liquid tank 9 and a plurality of cell factories to be inoculated 10; wherein, each pot is connected with an air filter 5 which is conveniently connected with a pressure vacuum pump to form negative pressure or positive pressure to provide power for the liquid flow. The three-way switch on the main pipeline is connected with the cell factory through the pipeline and the joint 1.
In this embodiment, the pipeline is a medical silicone tube; the joint is a PC joint; each tank is a plastic liquid storage tank; the tee joint is a T-shaped tee joint.
The above-mentioned embodiments are only examples of the present invention, and a plurality of modifications and replacements made by those skilled in the art without departing from the technical principles of the present invention should also be regarded as the protection scope of the present invention.
Claims (6)
1. The utility model provides a passage and cell harvest pipe-line system of cell factory which characterized in that includes a main line that constitutes by multistage pipeline and a plurality of three way switch (3) interval link to each other, and each three way switch (3) on the main line are connected respectively through the pipeline: a plurality of cell factories (2) to be treated, a waste liquid tank (4), a cleaning liquid tank (6), a digestion liquid tank (7), a termination liquid tank (8), a cell suspension liquid tank (9) and a plurality of cell factories (10) to be inoculated; wherein each pot is connected with an air filter (5) which is conveniently connected with a pressure vacuum pump to form negative pressure or positive pressure to provide power for liquid flow.
2. The conduit system of claim 1, wherein the conduit is a non-toxic silicone or plastic tube.
3. Tubing system according to claim 1, characterised in that the specifications and the number of the cell factories (2) to be treated and the cell factories (10) to be seeded can be set according to the actual situation, the cell factories can be 1 layer, 2 layers, 4 layers, 10 layers or 40 layers; the number of the cell factories (2) to be treated and the cell factories (10) to be seeded is 1-20.
4. Tubing system according to claim 1, characterized in that the three-way switch (3) on the main tubing is connected to the cell factory again via tubing and a fitting (1); wherein the joint (1) is a PC joint or a quick joint which can be butted with a cell factory.
5. The pipeline system according to claim 1, wherein the waste liquid tank (4), the cleaning liquid tank (6), the digestion liquid tank (7), the termination liquid tank (8) and the cell suspension liquid tank (9) can be selected from glass fiber port split bottles or plastic liquid tanks with different specifications according to production scale and actual conditions.
6. Piping system according to claim 1, characterized in that said three-way switch (3) regulates the liquid flow direction, comprising a "T" or "Y" type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022185260.4U CN212610703U (en) | 2020-09-30 | 2020-09-30 | Passage and cell harvesting pipeline system of cell factory |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022185260.4U CN212610703U (en) | 2020-09-30 | 2020-09-30 | Passage and cell harvesting pipeline system of cell factory |
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| CN212610703U true CN212610703U (en) | 2021-02-26 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350517A (en) * | 2022-01-14 | 2022-04-15 | 深圳赛动智造科技有限公司 | Intelligent stem cell culture equipment based on cell factory and culture method thereof |
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2020
- 2020-09-30 CN CN202022185260.4U patent/CN212610703U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350517A (en) * | 2022-01-14 | 2022-04-15 | 深圳赛动智造科技有限公司 | Intelligent stem cell culture equipment based on cell factory and culture method thereof |
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