CN215288823U

CN215288823U - Stem cell culture bottle for cell genetic engineering

Info

Publication number: CN215288823U
Application number: CN202022906063.7U
Authority: CN
Inventors: 向家兰
Original assignee: Guangxi Taimei Life Biotechnology Co ltd
Current assignee: Guangxi Taimei Life Biotechnology Co ltd
Priority date: 2020-12-07
Filing date: 2020-12-07
Publication date: 2021-12-24
Anticipated expiration: 2030-12-07

Abstract

The utility model relates to the technical field of stem cell culture, in particular to a stem cell culture bottle for cell genetic engineering, which comprises a bottle body and a bottle cap, the bottle cap is spirally connected with the bottle mouth of the bottle body, a culture plate is arranged in the bottle body, the culture plate is a spiral body, an air inlet pipe is arranged at the lower end of the bottle body in a penetrating way, the air inlet pipe is connected with air supply equipment, an air outlet pipe and an injection pipe are arranged on the bottle cap in a penetrating way, one end of the material injection pipe is rotatably connected with a sealing cover, a diversion trench is arranged on the culture plate, the culture medium can be injected into the upper end of the diversion trench and can flow downwards along the spiral direction of the culture plate, and then the culture medium is paved on the stem cell attaching surface of the whole culture plate, and the culture plate is arranged in the bottle body and is a spiral body, so that the attaching area of stem cell culture can be increased, and the using amount of the culture bottle is reduced.

Description

Stem cell culture bottle for cell genetic engineering

Technical Field

The utility model relates to a stem cell culture technical field, concretely relates to stem cell blake bottle for cell genetic engineering.

Background

The discovery of stem cells, a type of pluripotent cells with self-replicating ability, and the development of stem cell technology, as the latest research field of biotechnology, provide unique perspectives, methods and means for the treatment of human diseases, and provide new hopes and eosins for the treatment of human diseases, and will certainly lead to a revolution in medicine. Under certain conditions, it can be differentiated into various functional cells, has the potential function of regenerating various tissues and organs and human bodies, and is called as universal cells in the medical field.

However, the stem cell culture bottle has a technical bottleneck, that is, the amount of cells cultured in the stem cell culture bottle at one time cannot meet the requirement of the experiment, so that more than one cell culture bottle is required for cell culture, which causes the increase of cost and the waste of resources in the research and development process of medicines and medical devices.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

To prior art not enough, the utility model provides a stem cell blake bottle for cell genetic engineering has overcome prior art's not enough, and structural design is simple, the effectual problem of mentioning in the solution background art.

(II) technical scheme

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes:

the utility model provides a stem cell blake bottle for cell genetic engineering, includes bottle and bottle lid, bottle lid screwed connection is in the bottleneck department of bottle, be provided with the training plate in the bottle, the training plate sets up for the spirochaeta, the intake pipe is worn to be equipped with by the bottle lower extreme, the intake pipe is connected with air supply equipment, wear to be equipped with outlet duct and notes material pipe on the bottle lid, the one end rotation of notes material pipe is connected with sealed lid.

As an optimal technical scheme of the utility model, the central point of culture plate puts and wears to be equipped with the support column, support column and culture plate fixed connection.

As the utility model discloses a preferred technical scheme, the guiding gutter about running through is seted up to the week side of cultivateing the board, set up a plurality of drainage groove with the guiding gutter intercommunication on the cultivation board, the drainage groove is the concentric circles equidistance and distributes.

As an optimal technical scheme of the utility model, the one end that the bottle was kept away from to the intake pipe is installed the disinfection storehouse, there is a check valve one end that the intake pipe is close to the bottle through bolted connection.

As an optimized technical proposal of the utility model, the inner wall of the disinfection bin is provided with a disinfection cotton ball, and the inside of the disinfection cotton ball is provided with an active carbon ball.

As an optimal technical scheme of the utility model, there is the second check valve outlet duct one end near the bottle through bolted connection.

As an optimal technical scheme of the utility model, it is equipped with the sealing plug to annotate the embedded sealing plug that is equipped with of material pipe, the sealing plug is made by soft rubber materials.

(III) advantageous effects

The embodiment of the utility model provides a stem cell blake bottle for cell genetic engineering possesses following beneficial effect:

1. through set up the culture plate in the bottle, the culture plate sets up for the spirochaeta, can increase the area of adhering to of stem cell culture, reduces the use amount of blake bottle.

2. Through set up the guiding gutter on the culture plate, can pour into the culture medium into in the upper end of guiding gutter, the culture medium can be along with the spiral direction of culture plate down-flow, and then paves the stem cell adhesive surface on whole culture plate with the culture medium.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.

FIG. 1 is a schematic structural diagram of a stem cell culture flask for cell genetic engineering according to the present invention;

FIG. 2 is a perspective view of a culture plate in a stem cell culture flask for cell engineering according to the present invention;

FIG. 3 is a partial structure diagram of a disinfection chamber in a stem cell culture bottle for cell genetic engineering according to the present invention;

FIG. 4 is a cross-sectional view of a culture plate in a stem cell culture flask for cell engineering according to the present invention.

In the figure: 1. a bottle body; 2. culturing the plate; 201. a diversion trench; 202. a drainage groove; 3. a bottle body; 4. a disinfection bin; 5. an air inlet pipe; 6. an air outlet pipe; 7. a material injection pipe; 8. a sealing cover; 9. a sealing plug; 10. sterilizing cotton balls; 11. an activated carbon sphere; 12. a support pillar; 13. a first check valve; 14. a second one-way valve.

Detailed Description

The preferred embodiments of the present invention will be described below with reference to the accompanying drawings, and it should be understood that the preferred embodiments described herein are merely for purposes of illustration and explanation, and are not intended to limit the present invention:

as shown in fig. 1-4: the utility model provides a stem cell blake bottle for cell genetic engineering, includes bottle 1 and

bottle lid

3, 3 screwed connection of bottle lid are in the bottleneck department of bottle 1, are provided with culture plate 2 in the bottle 1, and culture plate 2 is the spirochaeta setting, and 1 lower extreme of bottle wears to be equipped with intake pipe 5, and intake pipe 5 is connected with air supply equipment, wears to be equipped with outlet duct 6 and notes material pipe 7 on the bottle lid 3, and the one end rotation of notes material pipe 7 is connected with sealed lid 8.

Wherein, the central point of culture plate 2 puts and wears to be equipped with support column 12, and support column 12 and culture plate 2 fixed connection can strengthen culture plate 2.

Wherein, the guiding gutter 201 about running through is seted up to the week side of culture plate 2, has seted up a plurality of drainage groove 202 with guiding gutter 201 intercommunication on the culture plate 2, and drainage groove 202 is the equidistance of concentric circles and distributes, can pave whole culture plate 2 with the culture medium drainage.

Wherein, the one end that the bottle 1 was kept away from to intake pipe 5 is installed and is disinfected storehouse 4, and the one end that intake pipe 5 is close to bottle 1 has first check valve 13 through bolted connection, can disinfect to the gas of carrying into.

Wherein, the inner wall of disinfection storehouse 4 is provided with aseptic cotton ball 10, and the inside of aseptic cotton ball 10 is provided with activated carbon ball 11, can disinfect and dry the gaseous of inputing.

Wherein, the one end that the outlet duct 6 is close to bottle 1 has second check valve 14 through bolted connection, can prevent that gas from flowing backward.

Wherein, the material injection pipe 7 is embedded with a sealing plug 9, and the sealing plug 9 is made of soft rubber material, so that gas can be prevented from flowing backwards.

The utility model discloses a theory of operation: rotating the sealing cover 8, using the syringe that is equipped with the culture medium to pierce through sealing plug 9, pour into the culture medium into guiding gutter 201 in, when the culture medium flows down, by drainage groove 202 drainage to the whole culture plate 2 of paving, gas supply unit is to intake pipe 5 conveying gas, and in gas got into bottle 1 through the filtration in disinfection storehouse 4, waste gas discharged from outlet duct 6 to accomplish the gaseous change to bottle 1 inside.

Finally, it should be noted that: in the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a stem cell blake bottle for cell genetic engineering, includes bottle (1) and bottle lid (3), bottle lid (3) screwed connection is in the bottleneck department of bottle (1), its characterized in that: be provided with in bottle (1) and cultivate board (2), cultivate board (2) are the spirochaeta setting, intake pipe (5) wear to be equipped with to bottle (1) lower extreme, intake pipe (5) are connected with the air supply equipment, wear to be equipped with outlet duct (6) and notes material pipe (7) on bottle lid (3), the one end rotation of notes material pipe (7) is connected with sealed lid (8).

2. The stem cell culture flask for cell genetic engineering according to claim 1, wherein: a support column (12) penetrates through the center of the culture plate (2), and the support column (12) is fixedly connected with the culture plate (2).

3. The stem cell culture flask for cell genetic engineering according to claim 1, wherein: the blast groove (201) about running through is seted up to the week side of cultivateing board (2), cultivate and set up a plurality of drainage groove (202) with blast groove (201) intercommunication on board (2), drainage groove (202) are concentric circles equidistance and distribute.

4. The stem cell culture flask for cell genetic engineering according to claim 1, wherein: the one end that bottle (1) was kept away from in intake pipe (5) is installed disinfection storehouse (4), there is first check valve (13) one end that intake pipe (5) are close to bottle (1) through bolted connection.

5. The stem cell culture flask for cell genetic engineering according to claim 4, wherein: the inner wall of the disinfection bin (4) is provided with a disinfection cotton ball (10), and the inside of the disinfection cotton ball (10) is provided with an active carbon ball (11).

6. The stem cell culture flask for cell genetic engineering according to claim 1, wherein: one end of the air outlet pipe (6) close to the bottle body (1) is connected with a second one-way valve (14) through a bolt.

7. The stem cell culture flask for cell genetic engineering according to claim 2, wherein: the material injection pipe (7) is embedded with a sealing plug (9), and the sealing plug (9) is made of soft rubber materials.