CN114921327A - Microbial spore's cultivation equipment - Google Patents

Microbial spore's cultivation equipment Download PDF

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Publication number
CN114921327A
CN114921327A CN202210298132.1A CN202210298132A CN114921327A CN 114921327 A CN114921327 A CN 114921327A CN 202210298132 A CN202210298132 A CN 202210298132A CN 114921327 A CN114921327 A CN 114921327A
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cylinder
support
coaxially
bottom end
matched
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CN202210298132.1A
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CN114921327B (en
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覃拔
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Chongqing Mocigeng Agricultural Development Co ltd
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Individual
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/52—Mobile; Means for transporting the apparatus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/04—Heat
    • A61L2/06—Hot gas
    • A61L2/07—Steam
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a culture device of microbial spores, and belongs to the technical field of microbial culture. An apparatus for culturing microbial spores, comprising: the culture device comprises a support, and a shell mechanism, a lifting mechanism, a rotary feeding mechanism and a control panel which are arranged on the support, wherein the lifting mechanism is coaxial with the shell mechanism, and the control panel is used for controlling the operation of the shell mechanism, the lifting mechanism and the rotary feeding mechanism.

Description

Microbial spore's cultivation equipment
Technical Field
The invention belongs to the technical field of microbial cultivation, and particularly relates to a microbial spore cultivation device.
Background
The microbial fertilizer is a living product containing microbial strains with special functions, the shelf life of the microbial fertilizer plays a crucial role in commercial development and successful application of the microbial fertilizer, and as is known, spores and steamed stuffed buns of microorganisms are more durable to store than thalli and mycelia, so living organisms in the microbial fertilizer should exist in the forms of spores and spores as far as possible, and the reproducibility is poor due to inconsistent fermentation conditions in the existing microbial spore culture process.
Disclosure of Invention
The invention aims to solve the problem of poor reproducibility caused by inconsistent fermentation conditions in the existing microbial spore culture process, and provides a microbial spore culture device.
In order to achieve the purpose, the invention adopts the following technical scheme:
an apparatus for culturing microbial spores, comprising: the support, set up casing mechanism, the mechanism of pulling, rotatory reinforced mechanism and control panel on the support, pull mechanism and the coaxial swing joint of casing mechanism, control panel fixed connection in the support one side that deviates from casing mechanism, rotatory reinforced mechanism fixed connection in the support one side that deviates from control panel, pull mechanism and support top fixed connection, casing mechanism and support fixed connection, control panel be used for control casing mechanism, pull the operation of mechanism and rotatory reinforced mechanism.
Preferably, the shell mechanism comprises a first cylinder which is vertically arranged and connected with the support, an exhaust pipe arranged at the bottom end outside the circumference of the first cylinder and a sealing cover coaxially arranged at the bottom end of the first cylinder, the first cylinder is hollow and has two ends provided with openings, the exhaust pipe extends along the radial direction of the first cylinder and is communicated with the inner cavity of the first cylinder, the bottom end of the first cylinder is coaxially provided with an annular groove, a plurality of first guide blocks are arranged in the annular groove along the circumferential direction, the top of the sealing cover is coaxially provided with an annular plate matched with the annular groove, the inner circular surface of the annular plate is provided with a first guide groove matched with the first guide blocks, the outer wall of the circumference of the annular plate is provided with a second threaded hole penetrating through the side wall of the annular plate, a first bolt is arranged in the second threaded hole in a matching manner, a through hole is coaxially arranged at the center of the sealing cover, a rubber plate is arranged in the through hole in a matching manner, and a fan is coaxially arranged at the bottom of the rubber plate, the bottom of the sealing cover is provided with a first threaded hole and a steam pipe communicated with the inner cavity of the first cylinder, a second bolt is arranged in the first threaded hole in a matched mode, and the steam pipe is provided with a valve.
Preferably, the rotary feeding mechanism comprises a second motor which is connected with the support and is vertically arranged, a speed reducer which is coaxially arranged at the bottom end of the second motor, a connecting shaft which is coaxially arranged at the bottom end of the speed reducer, and a second cylinder which is matched and arranged in an inner cavity of the first cylinder, the second cylinder is in threaded connection with the first cylinder, a gear is coaxially arranged at the bottom end of the connecting shaft, an outer gear ring which is meshed with the gear is coaxially sleeved outside the top end of the second cylinder, a plurality of second guide grooves are uniformly arranged outside the second cylinder at intervals along the circumferential direction, the second guide grooves extend along the length direction of the second cylinder, a second guide block which is matched with the second guide grooves is arranged on the inner circular surface of the outer gear ring, a bearing is coaxially sleeved at the outer bottom end of the outer gear ring, the outer ring of the bearing is connected with the support, a dropper which extends along the radial direction of the second cylinder is arranged at the bottom end of the inner circular surface of the second cylinder, and the dropper is connected with a feeding pipe which extends to the top end of the second cylinder, the bottom of the dropper suspension end is provided with a monitoring probe.
Preferably, the lifting mechanism comprises a spiral sheet arranged in an inner cavity of the second cylinder in a matching manner, a plurality of culture members arranged at even intervals along the extension direction of the spiral sheet, a pressure sensor and a temperature sensor arranged on the culture members, the spiral sheet is in threaded connection with the second cylinder, threads between the second cylinder and the first cylinder are matched with the spiral sheet, the culture members comprise a support plate arranged on the spiral sheet, a fixed groove formed at the top of the support plate and a culture dish arranged in the fixed groove in a matching manner, the dropper suspension end is positioned right above the culture dish, the bottom end of the spiral sheet is provided with a third threaded hole matched with the second bolt, the top end of the spiral sheet is provided with a rubber piston matched with the inner cavity of the second cylinder, one side of the top end of the rubber piston is provided with a sterile air pipe communicated with the inner cavity of the second cylinder, and the top end of the rubber piston is coaxially provided with a telescopic rod, the top end of the telescopic rod is coaxially provided with a first motor connected with the bracket.
Preferably, the fan, the first motor, the pressure sensor, the temperature sensor, the second motor and the monitoring probe are all electrically connected with a control panel and are controlled by the control panel to operate.
Preferably, the diameter of the external gear ring is twice of the diameter of the gear.
Compared with the prior art, the invention provides a microbial spore culture device, which has the following beneficial effects:
1. when the spiral plate feeding device is used, the sealing cover is firstly opened, then the first motor is controlled to rotate through the control panel, the first motor drives the spiral plate to rotate through the telescopic rod and the rubber piston, the spiral plate rotates downwards and moves out of the inner cavity of the second cylinder, when the spiral plate moves out completely, the first motor is closed, then a culture dish is added on the fixed groove, then the spiral plate is controlled to reset, the sealing cover is used for resealing, and the second bolt is rotated to enable the second bolt to be screwed into the third threaded hole to fix the spiral plate.
2. According to the invention, steam is introduced through the steam pipe, the rubber plate is turned over to enable the fan to face upwards, the fan is started to blow the steam upwards, rapid diffusion of the steam is ensured, the temperature and the air pressure are monitored through the pressure sensor and the temperature sensor, the internal cavity of the second cylinder is kept at 121 ℃ and 0.12MPa for continuous sterilization for 30 minutes, the valve is closed, then sterile air is introduced through the sterile air pipe to cool, redundant air is discharged through the exhaust pipe, then the sterile air pipe and the exhaust pipe are sealed, the empty space is ensured to be small by arranging the spiral sheet, the utilization efficiency is improved, efficient proceeding of sterilization and cooling is ensured, and the working time is reduced.
3. The invention controls the second motor to start, the second motor drives the outer gear ring to rotate through the reducer, the connecting shaft and the gear, the outer gear ring drives the second cylinder to spirally ascend, the second cylinder drives the dropper and the monitoring probe to spirally ascend, the dropper passes through each culture dish and conveys nutrient solution and spore suspension to the culture dish, the conveying amount of the nutrient solution is observed and controlled through the monitoring probe, the same culture conditions of the same batch are ensured, and errors are reduced.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a first structural diagram of the housing mechanism, the lifting mechanism and the rotary feeding mechanism of the present invention;
FIG. 3 is a second schematic structural view of the housing mechanism, the lifting mechanism and the rotary charging mechanism of the present invention;
FIG. 4 is a third schematic structural view of the housing mechanism, the lifting mechanism and the rotary feeding mechanism of the present invention;
FIG. 5 is a first structural view of a lifting mechanism and a rotary charging mechanism according to the present invention;
FIG. 6 is a second schematic structural view of a lifting mechanism and a rotary feeding mechanism of the present invention;
FIG. 7 is a schematic structural view of a lifting mechanism of the present invention;
FIG. 8 is a schematic view of the rotary charging mechanism of the present invention;
FIG. 9 is a schematic structural view of the housing mechanism, the lifting mechanism and the rotary charging mechanism of the present invention;
FIG. 10 is a schematic view of the housing mechanism and the lifting mechanism of the present invention;
fig. 11 is a schematic structural diagram of the housing mechanism of the present invention.
The numbering in the figures illustrates:
10. a support; 20. a housing mechanism; 210. a first cylinder; 211. a ring groove; 212. a first guide block; 220. an exhaust pipe; 230. a sealing cover; 231. a ring plate; 232. a first guide groove; 233. a through hole; 234. a first threaded hole; 235. a second threaded hole; 236. a first bolt; 240. a rubber plate; 250. a fan; 260. a second bolt; 270. a steam pipe; 271. a valve; 280. a third threaded hole; 30. a lifting mechanism; 310. a first motor; 320. a telescopic rod; 330. a rubber piston; 340. a sterile trachea; 350. a spiral sheet; 360. a culture member; 361. a support plate; 362. a fixing groove; 363. a culture dish; 370. a pressure sensor; 380. a temperature sensor; 40. a rotary feeding mechanism; 410. a second motor; 420. a speed reducer; 430. a connecting shaft; 440. a gear; 450. an outer ring gear; 451. a second guide block; 460. a bearing; 470. a second cylinder; 471. a second guide groove; 472. a dropper; 473. monitoring the probe; 50. a control panel.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the mechanism or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
As shown in fig. 1 to 11, an apparatus for culturing spores of a microorganism, comprising: the device comprises a support 10, a shell mechanism 20 arranged on the support 10, a lifting mechanism 30, a rotary feeding mechanism 40 and a control panel 50, wherein the lifting mechanism 30 is coaxially and movably connected with the shell mechanism 20, the control panel 50 is fixedly connected with one side of the support 10, which is far away from the shell mechanism 20, the rotary feeding mechanism 40 is fixedly connected with one side of the support 10, which is far away from the control panel 50, the lifting mechanism 30 is fixedly connected with the top of the support 10, the shell mechanism 20 is fixedly connected with the support 10, and the control panel 50 is used for controlling the operation of the shell mechanism 20, the lifting mechanism 30 and the rotary feeding mechanism 40.
The shell mechanism 20 includes a first cylinder 210 vertically arranged and connected with the bracket 10, an exhaust pipe 220 arranged at the bottom end outside the circumference of the first cylinder 210, and a sealing cover 230 coaxially arranged at the bottom end of the first cylinder 210, the first cylinder 210 is hollow and has two ends provided with openings, the exhaust pipe 220 extends along the radial direction of the first cylinder 210 and the exhaust pipe 220 is communicated with the inner cavity of the first cylinder 210, the bottom end of the first cylinder 210 is coaxially provided with a ring groove 211, a plurality of first guide blocks 212 are arranged in the ring groove 211 along the circumferential direction, the top of the sealing cover 230 is coaxially provided with a ring plate 231 matched with the ring groove 211, the inner surface of the ring plate 231 is provided with a first guide groove 232 matched with the first guide blocks 212, the circumferential outer wall of the ring plate 231 is provided with a second threaded hole 235 penetrating through the side wall of the ring plate 231, a first bolt 236 is arranged in the second threaded hole 235 in a matching manner, and a through hole 233 is coaxially arranged at the center of the sealing cover 230, the through hole 233 is internally provided with a rubber plate 240 in a matching manner, the bottom of the rubber plate 240 is coaxially provided with a fan 250, the bottom of the sealing cover 230 is provided with a first threaded hole 234 and a steam pipe 270 communicated with the inner cavity of the first cylinder 210, the first threaded hole 234 is internally provided with a second bolt 260 in a matching manner, and the steam pipe 270 is provided with a valve 271.
The rotary feeding mechanism 40 comprises a second motor 410 which is connected with the support 10 and is vertically arranged, a speed reducer 420 which is coaxially arranged at the bottom end of the second motor 410, a connecting shaft 430 which is coaxially arranged at the bottom end of the speed reducer 420, and a second cylinder 470 which is matched and arranged in the inner cavity of the first cylinder 210, wherein the second cylinder 470 is in threaded connection with the first cylinder 210, a gear 440 is coaxially arranged at the bottom end of the connecting shaft 430, an outer gear ring 450 which is meshed with the gear 440 is coaxially sleeved outside the top end of the second cylinder 470, a plurality of second guide grooves 471 are uniformly arranged outside the second cylinder 470 at intervals along the circumferential direction, the second guide grooves 471 extend along the length direction of the second cylinder 470, a second guide block 451 which is matched with the second guide grooves 471 is arranged on the inner circular surface of the outer gear ring 450, a bearing 460 is coaxially sleeved at the outer bottom end of the outer gear ring 450, the outer ring of the bearing 460 is connected with the support 10, a dropper 472 which extends along the radial direction of the second cylinder 470 is arranged at the bottom end of the inner circular surface of the second cylinder 470, the dropper 472 feeds through a filler tube attached to the top end of the second cylinder 470, and a monitor probe 473 is positioned at the bottom of the suspended end of the dropper 472.
The pulling mechanism 30 comprises a spiral piece 350 arranged in the inner cavity of the second cylinder 470 in a matching manner, a plurality of culture members 360 arranged at even intervals along the extending direction of the spiral piece 350, a pressure sensor 370 and a temperature sensor 380 arranged on the culture members 360, the spiral piece 350 is in threaded connection with the second cylinder 470, threads between the second cylinder 470 and the first cylinder 210 are matched with the spiral piece 350, the culture members 360 comprise a support plate 361 arranged on the spiral piece 350, a fixing groove 362 arranged on the top of the support plate 361 and a culture dish 363 arranged in the fixing groove 362 in a matching manner, the suspension end of the dropper 472 is positioned right above the culture dish 363, the bottom end of the spiral piece 350 is provided with a third threaded hole 280 matched with the second bolt 260, the top end of the spiral piece 350 is provided with a rubber piston 330 matched with the inner cavity of the second cylinder 470, one side of the top end of the rubber piston 330 is provided with a sterile air pipe 340 communicated with the inner cavity of the second cylinder 470, the top end of the rubber piston 330 is coaxially provided with a telescopic rod 320, and the top end of the telescopic rod 320 is coaxially provided with a first motor 310 connected with the bracket 10.
The fan 250, the first motor 310, the pressure sensor 370, the temperature sensor 380, the second motor 410 and the monitoring probe 473 are all electrically connected to the control panel 50 and controlled by the control panel 50 to operate.
More perfectly, the diameter of the external gear ring 450 is twice of that of the gear 440; a moment is added to facilitate the rotation of the second cylinder 470.
When the sealing cover 230 is firstly opened, the first motor 310 is controlled to rotate by the control panel 50, the first motor 310 drives the spiral piece 350 to rotate through the expansion link 320 and the rubber piston 330, the spiral piece 350 rotates and moves downwards and out of the inner cavity of the second cylinder 470, when the spiral piece 350 is completely moved out, the first motor 310 is closed, the culture dish 363 is added on the fixing groove 362, the spiral piece 350 is controlled to reset, the sealing cover 230 is used for resealing, and the second bolt 260 is rotated to enable the second bolt 260 to be screwed into the third threaded hole 280 to fix the spiral piece 350.
Let in steam through steam pipe 270, overturn rubber slab 240 and make fan 250 up, start fan 250, fan 250 blows steam upwards, guarantee the quick diffusion of steam, monitor temperature and atmospheric pressure through pressure sensor 370 and temperature sensor 380, keep 470 inner chambers of second drum 121 degrees centigrade, 0.12MPa lasts sterilization 30 minutes, close valve 271, later let in aseptic air through aseptic trachea 340 and cool down, unnecessary air passes through blast pipe 220 and discharges, sealed aseptic trachea 340 and blast pipe 220 afterwards, through setting up flight 350, it is little to guarantee vacant space, the utilization efficiency is improved, the high efficiency of disinfecting and cooling is gone on with having guaranteed, the operating time is reduced.
The second motor 410 is controlled to be started, the second motor 410 drives the outer gear ring 450 to rotate through the speed reducer 420, the connecting shaft 430 and the gear 440, the outer gear ring 450 drives the second cylinder 470 to spirally ascend, the second cylinder 470 drives the dropper 472 and the monitoring probe 473 to spirally ascend, the dropper 472 passes through each culture dish 363 and conveys the nutrient solution and the spore suspension to the culture dish 363, the conveying amount of the nutrient solution is observed and controlled through the monitoring probe 473, the same culture conditions of the same batch are ensured, and errors are reduced.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (6)

1. An apparatus for culturing microbial spores, comprising: the device comprises a support (10), a shell mechanism (20) arranged on the support (10), a lifting mechanism (30), a rotary feeding mechanism (40) and a control panel (50), wherein the lifting mechanism (30) is coaxially and movably connected with the shell mechanism (20), the control panel (50) is fixedly connected to one side, deviating from the shell mechanism (20), of the support (10), the rotary feeding mechanism (40) is fixedly connected to one side, deviating from the control panel (50), of the support (10), the lifting mechanism (30) is fixedly connected with the top of the support (10), the shell mechanism (20) is fixedly connected with the support (10), and the control panel (50) is used for controlling the operation of the shell mechanism (20), the lifting mechanism (30) and the rotary feeding mechanism (40).
2. An apparatus for the cultivation of microbial spores as claimed in claim 1, wherein: the shell mechanism (20) comprises a first cylinder (210) which is vertically arranged and connected with the support (10), an exhaust pipe (220) which is arranged at the bottom end outside the circumference of the first cylinder (210) and a sealing cover (230) which is coaxially arranged at the bottom end of the first cylinder (210), wherein the first cylinder (210) is hollow, two ends of the first cylinder (210) are provided with openings, the exhaust pipe (220) radially extends along the first cylinder (210), the exhaust pipe (220) is communicated with the inner cavity of the first cylinder (210), the bottom end of the first cylinder (210) is coaxially provided with a ring groove (211), a plurality of first guide blocks (212) are arranged in the ring groove (211) along the circumferential direction, the top of the sealing cover (230) is coaxially provided with a ring plate (231) matched with the ring groove (211), the inner circular surface of the ring plate (231) is provided with a first guide groove (232) matched with the first guide blocks (212), the circumferential outer wall of the ring plate (231) is provided with a second threaded hole (235) which penetrates through the side wall of the ring plate (231), the sealing structure is characterized in that a first bolt (236) is arranged in the second threaded hole (235) in a matching mode, a through hole (233) is coaxially formed in the center of the sealing cover (230), a rubber plate (240) is arranged in the through hole (233) in a matching mode, a fan (250) is coaxially arranged at the bottom of the rubber plate (240), a first threaded hole (234) and a steam pipe (270) communicated with the inner cavity of the first cylinder (210) are arranged at the bottom of the sealing cover (230), a second bolt (260) is arranged in the first threaded hole (234) in a matching mode, and a valve (271) is arranged on the steam pipe (270).
3. An apparatus for the cultivation of microbial spores as claimed in claim 2, wherein: the rotary feeding mechanism (40) comprises a second motor (410) which is connected with the support (10) and is vertically arranged, a speed reducer (420) which is coaxially arranged at the bottom end of the second motor (410), a connecting shaft (430) which is coaxially arranged at the bottom end of the speed reducer (420) and a second cylinder (470) which is matched and arranged in an inner cavity of the first cylinder (210), wherein the second cylinder (470) is in threaded connection with the first cylinder (210), a gear (440) is coaxially arranged at the bottom end of the connecting shaft (430), an outer gear ring (450) which is meshed with the gear (440) is coaxially sleeved outside the top end of the second cylinder (470), a plurality of second guide grooves (471) are uniformly arranged at intervals along the circumferential direction outside the second cylinder (470), the second guide grooves (471) extend along the length direction of the second cylinder (470), and second guide blocks (451) which are matched with the second guide grooves (471) are arranged on the inner circular surface of the outer gear ring (450), the bearing (460) is coaxially sleeved at the bottom end of the outer portion of the outer gear ring (450), the outer ring of the bearing (460) is connected with the support (10), a dropper (472) extending along the radial direction of the second cylinder (470) is arranged at the bottom end of the inner circular surface of the second cylinder (470), the dropper (472) feeds materials through a liquid feeding pipe extending to the top end of the second cylinder (470) in a connecting mode, and a monitoring probe (473) is arranged at the bottom of the suspension end of the dropper (472).
4. An apparatus for culturing microbial spores as claimed in claim 3, wherein: the lifting mechanism (30) comprises a spiral sheet (350) arranged in an inner cavity of the second cylinder (470) in a matching manner, a plurality of culture members (360) arranged at uniform intervals along the extending direction of the spiral sheet (350), and a pressure sensor (370) and a temperature sensor (380) arranged on the culture members (360), wherein the spiral sheet (350) is in threaded connection with the second cylinder (470), threads between the second cylinder (470) and the first cylinder (210) are matched with the spiral sheet (350), the culture members (360) comprise a support plate (361) arranged on the spiral sheet (350), a fixing groove (362) formed in the top of the support plate (361), and a culture dish (363) arranged in the fixing groove (362) in a matching manner, the suspension end of the dropper (472) is positioned right above the culture dish (363), and the bottom end of the spiral sheet (350) is provided with a third threaded hole (280) matched with the second bolt (260), the top end of the spiral sheet (350) is provided with a rubber piston (330) matched with the inner cavity of the second cylinder (470), one side of the top end of the rubber piston (330) is provided with a sterile air pipe (340) communicated with the inner cavity of the second cylinder (470), the top end of the rubber piston (330) is coaxially provided with a telescopic rod (320), and the top end of the telescopic rod (320) is coaxially provided with a first motor (310) connected with the support (10).
5. An apparatus for culturing spores of microorganisms according to claim 4, wherein: the fan (250), the first motor (310), the pressure sensor (370), the temperature sensor (380), the second motor (410) and the monitoring probe (473) are all electrically connected with the control panel (50) and controlled by the control panel (50) to operate.
6. An apparatus for culturing microbial spores as claimed in claim 3, wherein: the diameter of the outer gear ring (450) is twice that of the gear (440).
CN202210298132.1A 2022-03-24 2022-03-24 Microorganism spore's culture apparatus Active CN114921327B (en)

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