CN113801783A

CN113801783A - Large intestine group bacteria culture method based on laboratory

Info

Publication number: CN113801783A
Application number: CN202111119204.3A
Authority: CN
Inventors: 张玉霞; 王卫平; 汪翠翠
Original assignee: Tongling Tiantian Paper Technology Co ltd
Current assignee: Tongling Tiantian Paper Technology Co ltd
Priority date: 2021-09-18
Filing date: 2021-09-18
Publication date: 2021-12-17

Abstract

The invention discloses a laboratory-based large intestine group bacteria culture method, which relates to the technical field of large intestine group bacteria culture and comprises a culture shell, a pressure frame assembly, a lifting and blanking assembly, a rotating and bearing assembly, a heater, a vacuum machine and a one-way electromagnetic valve.

Description

Large intestine group bacteria culture method based on laboratory

Technical Field

The invention relates to the technical field of large intestine flora culture, in particular to a laboratory-based large intestine flora culture method.

Background

In the process of culturing the large intestine group bacteria, various culture mediums are usually prepared manually and refrigerated, then the generated single stock plant of the large intestine group bacteria is placed in an incubator for culture, so that the single stock plant of the large intestine group bacteria is generated rapidly, wherein because the culture mediums are prepared manually, when a plurality of culture mediums corresponding to different single stock plants of the large intestine group bacteria need to be prepared and a multi-culture-medium control group needs to be prepared simultaneously, the confusion of fillers and the low preparation efficiency of the culture mediums are easily caused by the manual operation mode, the subsequent rapid growth increase of the large intestine group bacteria is influenced, and the analysis and comparison of each single stock plant of the large intestine group bacteria at the later stage are not facilitated;

in view of the above technical drawbacks, a solution is proposed.

Disclosure of Invention

The invention aims to: by arranging the culture shell, the pressure frame assembly, the lifting cutting assembly, the rotating bearing assembly, the heater, the vacuum machine and the one-way electromagnetic valve, on the basis of realizing automatic production of the large intestine colony culture medium, the rapid culture work of the large intestine colony is further realized, so that the production efficiency and the generation efficiency of the large intestine colony culture are improved, and the problems that when the large intestine colony is cultured by the traditional manual method, the efficiency of manually producing a large amount of culture medium is too slow, and the colony of the large intestine colony is too slow in culture generation are solved; the differential preparation of the culture medium is realized, and the differential preparation of the culture medium can be realized by different contents of the materials added into the culture dish when the materials are loaded, so that the differential culture medium is obtained, the influence of different material contents on the generation of the large intestine flora is researched, the experiment is more convenient and simpler, and the research, analysis and recording are more convenient.

In order to achieve the purpose, the invention adopts the following technical scheme:

the method for culturing the large intestine group bacteria based on the laboratory comprises the following specific working steps:

after various material pipes for storing materials are installed on a pressure frame assembly, opening a sealing split door, inserting a culture dish into an installation opening of a rotary shell, closing the sealing split door to enable the interior of the culture shell to be in a sealed state, and then starting a vacuum machine and a heater to vacuumize and heat and sterilize the culture shell;

step two, after the culture shell is subjected to heating sterilization treatment and vacuum pumping, required culture medium condition information is input from an input display terminal, wherein the required culture medium condition information comprises a single name of the large intestine flora to be cultured, the number of culture media corresponding to the single name, and a material list and content required by the culture media corresponding to the single name;

after the required culture medium condition information is input, starting the pressure frame assembly to perform pressurization treatment on the material pipe, and then starting the lifting material cutting assembly and the rotating receiving assembly to enable the material in the material pipe to enter the linear feeding nozzle and then be fed into the culture dish through the linear feeding nozzle;

when a plurality of culture dishes are arranged, the rotating bearing component is started and drives the culture dishes to rotate, so that the culture dishes are sequentially fed by the linear feeding nozzles, after the culture dishes are sequentially fed by the linear feeding nozzles, the lifting cutting component switches the next linear feeding nozzle to feed the culture dishes again, and then the steps are repeated to switch the materials, so that the materials are fed into the culture dishes according to the required culture medium condition information until the required culture medium condition information is met, and the culture dish feeding work is finished;

step five, after the feeding work of the culture medium materials is finished, starting the lifting and blanking assembly and the rotating and receiving assembly to work, controlling the linear feeding nozzle to be inserted into the culture materials of the culture dish after the lifting and blanking assembly works, then controlling the annular shell to rotate in a reciprocating fixed angle by the rotating and receiving assembly, driving the culture dish to slightly return and deflect after the annular shell rotates in the reciprocating fixed angle, enabling the culture materials in the culture dish to vibrate and slightly stir the culture materials in the culture dish through the linear feeding nozzle, and therefore enabling the culture materials in the culture dish to be uniformly mixed;

step six, after culture materials in the culture dish are uniformly mixed, starting a heater to carry out heating sterilization treatment on the culture dish, emulsifying or galactizing treatment on a culture medium in the culture dish, then starting a vacuum machine to extract gas generated by emulsification, then closing the vacuum machine and the heater, then opening a one-way electromagnetic valve to flush clean cold air, cooling the culture medium in the culture dish, simultaneously preventing external miscellaneous gas from rapidly entering a culture shell after opening a sealing split door to pollute the culture medium in the culture dish, opening the sealing split door and taking out the culture dish when the culture medium in the culture dish is cooled to normal temperature, and carrying out refrigeration treatment on the culture dish containing the culture medium, thereby completing automatic and efficient preparation of the culture medium;

step seven, after the large intestine group bacteria need to be cultured, taking out a culture dish containing a culture medium, dripping single large intestine group bacteria original strains, inserting the culture dish into an installation opening of an annular shell, extruding and fixing the culture dish by a first extruding and fixing arc sleeve and a second extruding and fixing arc sleeve, starting a vacuum machine to vacuumize the culture dish to remove impure gases, starting a heater to keep the annular shell at a constant temperature, opening a one-way electromagnetic valve to input moisture into the annular shell, keeping the moisture in the culture shell constant, ensuring that the temperature and the humidity of the equipment are most suitable for the survival of the single large intestine group bacteria original strains, controlling a lifting material cutting assembly to switch corresponding materials when the culture medium materials in the culture dish are consumed or need to perform color reaction, starting a rotary receiving assembly to drive the culture dish to be positioned at a linear material feeding nozzle of the corresponding materials, then enabling the materials to enter the culture dish to perform filling work, ensuring the rapid propagation of the single original strain of the large intestine group bacteria.

The cultivation equipment of the large intestine group bacteria cultivation method based on the laboratory comprises a cultivation shell, a pressure frame assembly, a lifting cutting assembly and a rotating receiving assembly, wherein the pressure frame assembly is fixedly arranged at the top of the cultivation shell, the lifting cutting assembly is slidably arranged in the cultivation shell, the rotating receiving assembly is rotatably arranged in the cultivation shell, the pressure frame assembly and the lifting cutting assembly are in through connection through a hose, the rotating receiving assembly is arranged under the lifting cutting assembly, and the pressure frame assembly, the lifting cutting assembly and the rotating receiving assembly are located on the same central axis.

Further, the pressure frame subassembly includes rectangle support, pressure boost spring, material pipe, air cylinder, pressure plate and pressure pole, the fixed top of cultivateing the casing that locates of rectangle support, the pressure plate slides and locates in the rectangle support and rather than the butt, and the bottom mounting of pressure plate has pressure boost spring, the installation snap ring is installed to the diapire of rectangle support, the bottom through connection of installation snap ring has the worker shape connecting pipe, air cylinder fixes the center department of locating the rectangle support diapire, the top and the pressure plate fixed connection of pressure pole, its other end and air cylinder's piston shaft fixed connection, the material pipe is installed between installation snap ring and pressure boost spring, the top of material pipe is located to the pressure boost spring housing and rather than the butt.

Furthermore, sliding bolts are fixedly arranged on two sides of the pressure plate, slideways matched with the sliding bolts are formed in two side walls of the rectangular support, and the sliding bolts penetrate through the slideways of the rectangular support in a sliding mode.

Further, protruding and discharge conduit are inserted to the material pipe including body, piston ring, piston rod, T type, the fixed bottom of locating the body of discharge conduit, the piston ring slide locate in the body and with the inner wall butt of piston ring, the roof that the body was run through to the bottom of piston rod extends to its inside and with piston ring fixed connection, and protruding bottom fixed connection is inserted with the T type on its top, the T type is inserted protruding top and is inserted in the pressure boost spring, and the pressure boost spring housing locate the T type and insert protruding top and rather than the butt, discharge conduit joint is in the installation snap ring, and has the cushion that increases the leakproofness between discharge conduit and the installation snap ring, and the setting can be dismantled to the material pipe, is convenient for change the different thing of installation.

Furthermore, the lifting and blanking assembly comprises an annular shell, a vacuum machine, a heater, a one-way electromagnetic valve, an inner blanking annular plate, a lifting cylinder, a lifting rod, a first rotating rod and a switching motor, wherein the lifting cylinder is fixedly arranged on the top wall of the culture shell, one end of the lifting rod is fixedly connected with a piston shaft of the lifting cylinder, the other end of the lifting rod is fixed at the center of the top end of the annular shell, the switching motor is fixedly arranged at the bottom end of the annular shell, the inner blanking annular plate is rotatably arranged in the annular shell and is abutted against the annular shell, one end of the first rotating rod is fixedly connected with the inner blanking annular plate, the other end of the first rotating rod penetrates through the inner wall of the annular shell to extend to the outside of the annular shell and is fixedly connected with an output shaft of the switching motor, the annular shell is provided with a blanking feed hole and a blanking discharge hole, the blanking feed hole is communicated with the I-shaped connecting pipe through a hose, and the blanking feed hole and the blanking discharge hole are provided with a plurality of blanking holes, the utility model discloses a blank feed hole, including the blank discharge hole, the blank feed hole is used for the axis of annular shell to distribute according to annular array as the centre of a circle, and blank feed hole and blank discharge hole correspond the setting, interior blank crown plate has seted up a route and has switched the hole, the route switches the hole respectively with blank feed hole and blank discharge hole activity through connection, blank discharge hole through connection has linear feed nozzle, linear feed nozzle is fixed to be located the bottom of annular shell, and linear feed nozzle and blank discharge hole through corresponding quantity setting, and is a plurality of linear feed nozzle encircles in the switching motor ground outside, the outside symmetry of annular shell is equipped with spacing slide bar, spacing slide bar slides with the culture shell body and sets up, be equipped with sealed elastic ring between annular shell and the interior blank crown plate, sealed elastic ring offer with the through-hole of blank discharge hole adaptation.

Furthermore, the rotating receiving assembly comprises a rotating outer shell, a second rotating rod, a rotating motor, a supporting inner shell, a third rotating rod, a first extruding and fixing arc sleeve and a second extruding and fixing arc sleeve, the rotating outer shell is rotatably arranged on the culture shell, the rotating motor is fixedly arranged at the bottom end of the culture shell, one end of the second rotating rod is fixedly connected with an output shaft of the rotating motor, the other end of the second rotating rod is fixedly arranged at the center of the bottom of the rotating outer shell, the supporting inner shell is fixedly arranged in the rotating outer shell, the top surface of the rotating outer shell is provided with an installation port for placing a culture dish, the first extruding and fixing arc sleeve and the second extruding and fixing arc sleeve are arranged at the installation port of the rotating outer shell, the first extruding and fixing arc sleeve is in clearance fit with the second extruding and fixing arc sleeve, and the first extruding and fixing arc sleeve is fixedly arranged in the rotating outer shell;

the second crowded solid arc cover is equipped with crowded solid slide bar in one side of first crowded solid arc cover is fixed mutually, crowded solid spring has been cup jointed to the outer end of crowded slide bar, and crowded solid slide bar keep away from the one end of the crowded solid arc cover of second and run through the support inner shell and extend to its inside and fixedly connected with pull ring, the pull ring winding is fixed with the stay cord, the third bull stick rotates the center department of locating the support inner shell, the one end of third bull stick is passed through the bearing and is rotated the shell and be connected, and the other end of third bull stick runs through the roof that rotates the shell and extend to its outside and fixedly connected with commentaries on classics handle, the one end of pull ring is kept away from to the stay cord twines in the outer end of third bull stick, and stay cord and third bull stick fixed connection.

Furthermore, the mounting opening, the first extrusion fixing arc sleeve, the second extrusion fixing arc sleeve, the extrusion fixing slide rod, the extrusion fixing spring, the pull ring and the pull rope form a group of self-locking dismounting and pulling structures, the self-locking dismounting and pulling structures are provided with a plurality of self-locking dismounting and pulling structures, and the self-locking dismounting and pulling structures are distributed according to an annular array by taking the central point of the rotating shell as the center.

Furthermore, a partition plate and a heat insulation plate are fixedly arranged in the culture shell, and the culture shell is hinged with a sealing split door.

In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:

1. according to the invention, by arranging the culture shell, the pressure frame assembly, the lifting and blanking assembly, the rotating and receiving assembly, the heater, the vacuum machine and the one-way electromagnetic valve, on the basis of realizing automatic preparation of the large intestine group bacteria culture medium, the rapid culture work of large intestine group bacteria colonies is further realized, so that the production efficiency and the generation efficiency of the large intestine group bacteria culture are improved, and the problems that the efficiency of manually preparing and producing a large amount of culture medium is too slow and the colony of the large intestine group bacteria culture is too slow when the large intestine group bacteria are cultured by the traditional method are solved;

2. the differential preparation of the culture medium is realized through equipment, and the differential preparation of the culture medium can obtain the differential culture medium according to different contents of materials added into a culture dish when the materials are fed, so that the influence of different material contents on the generation of the large intestine group bacteria is researched, the experiment is more convenient and simpler, and the research, the analysis and the recording are more convenient.

Drawings

FIG. 1 shows a front view of a cultivation apparatus provided according to the invention;

FIG. 2 is a schematic view showing an internal structure of a cultivation apparatus provided according to the present invention;

FIG. 3 illustrates a cross-sectional view at a pressure frame assembly provided in accordance with the present invention;

figure 4 shows an enlarged cross-sectional view at the material tube provided according to the invention;

FIG. 5 illustrates a schematic structural view of a lifting blanking assembly provided in accordance with the present invention;

FIG. 6 illustrates a top view of a spin bearing assembly provided in accordance with the present invention;

FIG. 7 illustrates a cross-sectional view of a spin bearing assembly provided in accordance with the present invention;

FIG. 8 shows an enlarged view at A of FIG. 7;

illustration of the drawings: 1. culturing the shell; 2. a pressure frame assembly; 3. lifting and lowering the blanking assembly; 4. rotating the receiving assembly; 5. a heater; 6. a vacuum machine; 7. a control panel; 8. a start button; 9. inputting a display terminal; 101. a partition panel; 102. a heat insulation plate; 103. a supporting seat; 104. sealing the split doors; 105. a one-way solenoid valve; 201. a rectangular bracket; 202. a pressurizing spring; 203. a material pipe; 204. a pressure cylinder; 205. a pressure lever; 206. a pressure plate; 207. an I-shaped connecting pipe; 208. installing a snap ring; 209. a sliding bolt; 210. a slideway; 2031. a pipe body; 2032. a piston ring; 2033. a piston rod; 2034. t-shaped insertion protrusions; 2035. a discharge conduit; 301. an annular housing; 302. an inner blanking ring plate; 303. a material cutting feed hole; 304. a material cutting and discharging hole; 305. a passage switching hole; 306. a lifting cylinder; 307. a lifting rod; 308. a first rotating lever; 309. switching the motors; 310. a linear feeding nozzle; 311. a sealing elastic ring; 312. a limiting slide bar; 401. rotating the housing; 402. a second rotating rod; 403. rotating the motor; 404. an installation port; 405. a first extruded arc sleeve; 406. second extruding and fixing the arc sleeve; 407. extruding and fixing the slide bar; 408. extruding and fixing the spring; 409. a pull ring; 410. pulling a rope; 411. a third rotating rod; 412. turning a handle; 413. supporting the inner shell.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1:

as shown in fig. 1-8, the laboratory-based apparatus for culturing coliform bacteria comprises a culture housing 1, a pressure frame assembly 2 fixedly disposed on the top of the culture housing 1, a lifting and blanking assembly 3 slidably disposed in the culture housing 1, and a rotating and receiving assembly 4 rotatably disposed in the culture housing 1, wherein the pressure frame assembly 2 and the lifting and blanking assembly 3 are connected through a hose, the rotating and receiving assembly 4 is disposed under the lifting and blanking assembly 3, the pressure frame assembly 2, the lifting and blanking assembly 3, and the rotating and receiving assembly 4 are located on the same central axis, the pressure frame assembly 2 is used for placing and pressurizing added materials, the rotating and receiving assembly 4 is used for placing and clamping culture dishes, and the lifting and blanking assembly 3 is used for switching material types and matching with the rotating and receiving assembly 4 to deliver the materials into the culture dishes;

the culture shell 1 is internally and fixedly provided with a partition plate 101 and a heat insulation plate 102, a vacuum machine 6 and a heater 5 are fixedly arranged between the culture shell 1 and the heat insulation plate 102, the vacuum machine 6 is communicated with a cavity between the heat insulation plate 102 and the culture shell 1 so as to extract air at the position of a rotating receiving assembly 4 and enable the air to be in an oxygen-free environment, the heater 5 is used for heating the cavity between the heat insulation plate 102 and the culture shell 1 at a high temperature and removing sundry bacteria in equipment, the outer side of the culture shell 1 is communicated with two one-way electromagnetic valves 105, the one-way electromagnetic valves 105 are respectively used for introducing clean cold air and moisture into the culture shell 1, and four corners of the bottom of the culture shell 1 are provided with supporting seats 103;

the cultivation shell 1 is hinged with a sealed split door 104, a control panel 7 is mounted on the sealed split door 104, a starting button 8 and an input display terminal 9 are mounted on the control panel 7, the control panel 7 is used for controlling internal parts of the control panel to automatically operate, the starting button 8 ensures that equipment is powered on to operate and enables the control panel 7 to be started, and the input display terminal 9 is used for inputting material requirement parameters or selecting common materials or importing materials from a mobile phone based on the Internet;

the pressure frame component 2 comprises a rectangular support 201, a pressurizing spring 202, a material pipe 203, a pressure cylinder 204, a pressure plate 206 and a pressure rod 205, the rectangular support 201 is fixedly arranged at the top end of the culture shell 1, the pressure plate 206 is slidably arranged in the rectangular support 201 and is abutted against the rectangular support 201, the pressurizing spring 202 is fixedly arranged at the bottom end of the pressure plate 206, an installation snap ring 208 is arranged on the bottom wall of the rectangular support 201, the bottom end of the installation snap ring 208 is connected with an I-shaped connecting pipe 207 in a penetrating way, the pressure cylinder 204 is fixedly arranged at the center of the bottom wall of the rectangular support 201, the top end of the pressure rod 205 is fixedly connected with the pressure plate 206, the other end of the pressure rod is fixedly connected with a piston shaft of the pressure cylinder 204, the material pipe 203 is arranged between the installation snap ring 208 and the pressurizing spring 202, the pressurizing spring 202 is sleeved at the top end of the material pipe 203 and is abutted against the top end of the material pipe 203 to be in an extruding state, and primarily pressurizes the material of the material pipe 203, the two side walls of the rectangular support 201 are provided with slideways 210, the two sides of the pressure plate 206 are fixedly provided with sliding bolts 209, the sliding bolts 209 penetrate through the slideways 210 of the rectangular support 201 in a sliding manner, and the sliding bolts 209 ensure the stability of the pressure plate 206 during lifting;

the material pipe 203 comprises a pipe body 2031, piston rings 2032, piston rods 2033, a T-shaped insertion protrusion 2034 and a discharge pipe 2035, the discharge pipe 2035 is fixedly arranged at the bottom end of the pipe body 2031, the piston rings 2032 are slidably arranged in the pipe body 2031 and are abutted against the inner wall of the piston rings 2032, the bottom end of the piston rods 2033 penetrates through the top wall of the pipe body 2031 to extend into the pipe body and are fixedly connected with the piston rings 2032, the top end of the piston rings 2031 is fixedly connected with the bottom end of the T-shaped insertion protrusion 2034, the top end of the T-shaped insertion protrusion 2034 is inserted into a pressurizing spring 202, the pressurizing spring 202 is sleeved at the top end of the T-shaped insertion protrusion 2034 and is abutted against the top end of the T-shaped insertion protrusion 2034, the discharge pipe 2035 is clamped in the installation clamp ring 208, an elastic pad for increasing the sealing performance is arranged between the discharge pipe 2035 and the installation clamp ring 208, and the material pipe 203 is detachably arranged, so that different objects can be conveniently replaced and installed;

starting the pressure cylinder 204 to work and controlling the piston shaft to retract, driving the pressure plate 206 fixed with the pressure cylinder 204 to move downwards after the piston shaft of the pressure cylinder 204 retracts, extruding the pressurizing spring 202 after the pressure plate 206 moves downwards, enabling the pressurizing spring 202 to elastically deform, extruding the T-shaped inserting protrusion 2034 after the pressurizing spring 202 elastically deforms, enabling the T-shaped inserting protrusion 2034 to have a downward movement trend, enabling the piston rod 2033 to have a downward movement trend after the T-shaped inserting protrusion 2034 has the downward movement trend, enabling the piston ring 2032 to have a downward movement trend after the piston rod 2033 has the downward movement trend, enabling the piston ring 2032 to be slidably arranged in the pipe body 2031 and always abut against the material, and therefore pressurizing the material in the pipe body 2031, wherein the material is liquid, so that the material can be fed in time when needed, and the feeding of the emulsion material is not facilitated only by gravity;

the lifting and blanking assembly 3 comprises an annular shell 301, an inner blanking annular plate 302, a lifting cylinder 306, a lifting rod 307, a first rotating rod 308 and a switching motor 309, wherein the lifting cylinder 306 is fixedly arranged on the top wall of the culture shell 1, one end of the lifting rod 307 is fixedly connected with a piston shaft of the lifting cylinder 306, the other end of the lifting rod is fixed at the center of the top end of the annular shell 301, the switching motor 309 is fixedly arranged at the bottom end of the annular shell 301, the inner blanking annular plate 302 is rotatably arranged in the annular shell 301 and is abutted against the annular shell 301, one end of the first rotating rod 308 is fixedly connected with the inner blanking annular plate 302, the other end of the first rotating rod penetrates through the inner wall of the annular shell 301 to extend to the outside of the annular shell and is fixedly connected with an output shaft of the switching motor 309, the annular shell 301 is provided with a blanking feed hole 303 and a blanking discharge hole 304, the blanking feed hole 303 is communicated with the I-shaped connecting pipe 207 through a hose, the plurality of the blanking feed holes 303 and the blanking discharge holes 304, the blanking feed holes 303 are distributed in an annular array by taking the central axis of the annular shell 301 as the center of a circle, the cutting material inlet hole 303 and the cutting material outlet hole 304 are correspondingly arranged, the inner cutting ring plate 302 is provided with a passage switching hole 305, the passage switching hole 305 is respectively and movably communicated with the cutting material inlet hole 303 and the cutting material outlet hole 304, the cutting material outlet hole 304 is communicated with a linear feeding nozzle 310, the linear feeding nozzle 310 is fixedly arranged at the bottom end of the annular shell 301, the linear feeding nozzles 310 and the cutting discharge holes 304 are arranged in a corresponding number in a penetrating manner, the linear feeding nozzles 310 surround the outer side of the switching motor 309, limiting slide rods 312 are symmetrically arranged on the outer side of the annular shell 301, the limiting slide rods 312 are arranged in a sliding manner with the culture shell 1, a sealing elastic ring 311 is arranged between the annular shell 301 and the inner cutting ring plate 302, a through hole matched with the cutting discharge hole 304 is formed in the sealing elastic ring 311, and the sealing elastic ring 311 ensures the sealing performance of the inner cutting ring plate 302 during rotation;

when the pressure frame assembly 2 pressurizes materials, the lifting cylinder 306 is started to work and controls the piston shaft to move outwards, the piston shaft moves outwards and then drives the lifting rod 307 fixed with the piston shaft to move outwards, the lifting rod 307 moves outwards and then drives the annular shell 301 fixed with the lifting rod 307 and parts on the annular shell 301 to move downwards together until the linear feeding nozzle 310 is positioned right above a culture dish on the rotating receiving assembly 4, the linear feeding nozzle 310 can be partially inserted into the culture dish or not, when the linear feeding nozzle 310 is inserted into the culture dish and the rotating receiving assembly 4 needs to rotate, the linear feeding nozzle 310 is controlled to ascend for a certain distance, and the linear feeding nozzle 310 reciprocates to prevent the linear feeding nozzle 310 from colliding with the culture dish;

then, the switching motor 309 is started to work and the output shaft of the switching motor 309 is controlled to rotate by a certain angle, after the output shaft of the switching motor 309 rotates by a certain angle, the first rotating rod 308 fixed with the first rotating rod is driven to rotate, the inner cutting ring plate 302 fixed with the first rotating rod is driven to rotate, after the inner cutting ring plate 302 rotates, the position of the passage switching hole 305 is changed, the non-passage of the cutting feed hole 303 and the cutting discharge hole 304 is changed into the passage state, and then the time of the passage state is controlled, so that the material feeding amount in the material pipe 203 is controlled;

the rotating receiving component 4 comprises a rotating outer shell 401, a second rotating rod 402, a rotating motor 403, a supporting inner shell 413, a third rotating rod 411, a first extruding and fixing arc sleeve 405 and a second extruding and fixing arc sleeve 406, the rotating outer shell 401 is rotatably arranged on the culture shell 1, the rotating motor 403 is fixedly arranged at the bottom end of the culture shell 1, one end of the second rotating rod 402 is fixedly connected with an output shaft of the rotating motor 403, the other end of the second rotating rod is fixedly arranged at the center of the bottom of the rotating outer shell 401, the supporting inner shell 413 is fixedly arranged in the rotating outer shell 401, the top surface of the rotating outer shell 401 is provided with an installation port 404 for placing a culture dish, the first extruding and fixing arc sleeve 405 and the second extruding and fixing arc sleeve 406 are arranged at the installation port 404 of the rotating outer shell 401, the first extruding and fixing arc sleeve 405 is in clearance fit with the second extruding and fixing arc sleeve 406, and the first extruding and fixing arc sleeve 405 is fixedly arranged in the rotating outer shell 401;

one side, opposite to the first extrusion arc sleeve 405, of the second extrusion arc sleeve 406 is fixedly provided with an extrusion sliding rod 407, the outer end of the extrusion sliding rod 407 is sleeved with an extrusion spring 408, one end, away from the second extrusion arc sleeve 406, of the extrusion sliding rod 407 penetrates through the support inner shell 413 to extend into the support inner shell and is fixedly connected with a pull ring 409, the pull ring 409 is wound and fixed with a pull rope 410, the third rotating rod 411 is rotatably arranged at the center of the support inner shell 413, one end of the third rotating rod 411 is rotatably connected with the rotating outer shell 401 through a bearing, the other end of the third rotating rod 411 penetrates through the top wall of the rotating outer shell 401 to extend out of the rotating outer shell 401 and is fixedly connected with a rotating handle 412, one end, away from the pull ring 409, of the pull rope 410 is wound on the outer end of the third rotating rod 411, and the pull rope 410 is fixedly connected with the third rotating rod 411;

the mounting port 404, the first extrusion fixing arc sleeve 405, the second extrusion fixing arc sleeve 406, the extrusion fixing slide rod 407, the extrusion fixing spring 408, the pull ring 409 and the pull rope 410 form a group of self-locking dismounting and pulling structures, the number of the self-locking dismounting and pulling structures is multiple, and the self-locking dismounting and pulling structures are distributed in an annular array by taking the central point of the rotating shell 401 as the center;

inserting the culture dish into the mounting opening 404, enabling the second extrusion fixing arc sleeve 406 to extrude the culture dish under the reverse acting force of the extrusion fixing spring 408, enabling the culture dish to be stably placed in the rotating shell 401, starting the switching motor 309 and controlling the output shaft of the switching motor to rotate after the culture dish is placed in the rotating shell 401, driving the second rotating rod 402 fixed with the switching motor 309 to rotate after the output shaft of the switching motor 309 rotates, driving the rotating shell 401 to rotate and driving the culture dish on the rotating shell 401 to rotate after the second rotating rod 402 rotates, and switching the position of the culture dish, so that feeding work of different materials is completed by matching with the pressure frame assembly 2 and the lifting material cutting assembly 3;

after feeding is completed, the switching motor 309 stops working, then the outlet of the culture dish is blocked, when the outlet of the culture dish is blocked, the rotating handle 412 is stirred to rotate, the rotating handle 412 drives the third rotating rod 411 fixed with the rotating handle to rotate, the third rotating rod 411 rotates to enable the pull rope 410 to be wound on the outer surface of the third rotating rod 411, the pull rope 410 is wound on the outer surface of the third rotating rod 411 to enable the pull rope 410 to be shortened, so that the pull ring 409 is pulled to move towards the direction of the third rotating rod 411, the pull ring 409 drives the extrusion fixing slide rod 407 fixed with the pull ring to move towards the direction of the third rotating rod 411, the extrusion fixing slide rod 407 moves towards the direction of the third rotating rod 411 to drive the second extrusion fixing arc sleeve 406 to move towards the direction of the supporting inner shell 413, and the gap between the second extrusion fixing arc sleeve 406 and the first extrusion fixing arc sleeve 405 is enlarged after the second extrusion fixing arc sleeve 406 moves towards the direction of the supporting inner shell 413, and therefore the culture dish is easily taken.

Example 2:

based on example 1, the laboratory-based method for automatically and efficiently preparing a culture medium for coliform bacteria comprises:

after various material pipes 203 for storing materials are installed on a pressure frame assembly 2, opening a sealing split door 104, inserting a culture dish into an installation opening 404 of a rotary shell 401, closing the sealing split door 104 to enable the interior of a culture shell 1 to be in a sealing state, and then starting a vacuum machine 6 and a heater 5 to carry out vacuumizing and heating sterilization treatment on the culture shell 1;

step two, after the culture shell 1 is subjected to heating sterilization treatment and vacuum pumping, required culture medium condition information is input from the input display terminal 9, wherein the required culture medium condition information comprises a single name required to be cultured by the large intestine flora, the number of culture media corresponding to the single name, and a material list and content required by the culture media corresponding to the single name;

step three, after the required culture medium condition information is input, the pressure frame assembly 2 is started to perform pressurization treatment on the material pipe 203, then the lifting and blanking assembly 3 and the rotating receiving assembly 4 are started, so that the material in the material pipe 203 enters the linear feeding nozzle 310 and is fed into the culture dish through the linear feeding nozzle 310;

step four, when a plurality of culture dishes are arranged, the rotating receiving component 4 is started and drives the culture dishes to rotate, so that the culture dishes are sequentially fed by the linear feeding nozzle 310, after the culture dishes are sequentially fed by the linear feeding nozzle 310, the lifting blanking component 3 switches the linear feeding nozzle 310 to feed the culture dishes again, and then the steps are repeated to switch the materials, so that the materials are fed into the culture dishes according to the required culture medium condition information until the required culture medium condition information is met, and the culture dish feeding work is finished;

step five, after the feeding work of the culture medium materials is finished, starting the lifting and lowering material feeding assembly 3 and the rotating and receiving assembly 4 to work, controlling the linear feeding nozzle 310 to be inserted into the culture materials in the culture dish after the lifting and lowering material feeding assembly 3 works, then controlling the annular shell 301 to rotate in a reciprocating fixed angle by the rotating and receiving assembly 4, driving the culture dish to slightly return and deflect after the annular shell 301 rotates in a reciprocating fixed angle, enabling the culture materials in the culture dish to vibrate and slightly stir the culture materials in the culture dish through the linear feeding nozzle 310, and accordingly enabling the culture materials in the culture dish to be uniformly mixed;

step six, after culture materials in the culture dish are uniformly mixed, starting a heater 5 to perform heating sterilization treatment on the culture dish, emulsifying or galactizing the culture medium in the culture dish, then starting a vacuum machine 6 to extract gas generated by emulsification, then closing the vacuum machine 6 and the heater 5, then opening a one-way electromagnetic valve 105 to flush clean cold air, cooling the culture medium in the culture dish, simultaneously preventing the foreign gas outside the culture medium after opening a sealing double-door 104 from rapidly entering a culture shell 1 to pollute the culture medium in the culture dish, opening the sealing double-door 104 and taking out the culture dish when the culture medium in the culture dish is cooled to normal temperature, and performing refrigeration treatment on the culture dish containing the culture medium, thereby completing automatic and efficient production of the culture medium;

example 3:

based on the embodiment 1 and the embodiment 2, the method for rapidly culturing and propagating the large intestine group bacteria comprises the following steps:

when the large intestine group bacteria need to be cultured, taking out a culture dish containing a culture medium, dripping single large intestine group bacteria original strains, inserting the culture dish into the mounting port 404 of the annular shell 301, extruding and fixing the culture dish by the first extruding and fixing arc sleeve 405 and the second extruding and fixing arc sleeve 406, starting the vacuum machine 6 to vacuumize the culture dish to remove impure gases, starting the heater 5 to keep the annular shell 301 at a constant temperature, opening the one-way electromagnetic valve 105 to input moisture into the annular shell, keeping the moisture in the culture shell 1 constant, ensuring that the temperature and the humidity of the equipment are most suitable for the existence of the single large intestine group bacteria original strains, controlling the lifting and cutting component 3 to switch corresponding materials when the culture medium materials in the culture dish are consumed or need to perform color reaction, starting the rotating and receiving component 4 to drive the culture dish to be positioned at the linear feeding nozzle 310 of the corresponding materials, then enabling the materials to enter the culture dish to perform filling work, ensuring the rapid propagation of the single original strain of the large intestine group bacteria.

With reference to embodiments 1 to 3, the invention further realizes the rapid cultivation of the bacterial colonies of the large intestine group bacteria on the basis of realizing the automatic preparation of the large intestine group bacteria culture medium by arranging the culture shell 1, the pressure frame assembly 2, the lifting and blanking assembly 3, the rotating and receiving assembly 4, the heater 5, the vacuum machine 6 and the one-way solenoid valve 105, thereby improving the production efficiency and the generation efficiency of the cultured large intestine group bacteria, and solving the problems that the efficiency of artificially producing a large amount of culture medium is too slow and the bacterial colonies of the cultured large intestine group bacteria are too slow when the traditional large intestine group bacteria are cultured manually;

the differential preparation of the culture medium is realized through equipment, and the differential preparation of the culture medium can obtain the differential culture medium according to different contents of materials added into a culture dish when the materials are fed, so that the influence of different material contents on the generation of the large intestine group bacteria is researched, and the experiment is more convenient and simpler.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.

Claims

1. The method for culturing the large intestine group bacteria based on the laboratory is characterized by comprising the following specific working steps:

after various material pipes (203) for storing materials are installed on a pressure frame assembly (2), opening a sealing split door (104), inserting a culture dish into an installation opening (404) of a rotary shell (401), closing the sealing split door (104) to enable the interior of a culture shell (1) to be in a sealing state, and then starting a vacuum machine (6) and a heater (5) to carry out vacuumizing and heating sterilization treatment on the culture shell (1);

step two, after the culture shell (1) is subjected to heating sterilization treatment and vacuum pumping, required culture medium condition information is input from the input display terminal (9), wherein the required culture medium condition information comprises a single name of the large intestine flora to be cultured, the number of culture media corresponding to the single name, a material list and content required by the culture media corresponding to the single name;

step three, after the required culture medium condition information is input, starting a pressure frame assembly (2) to pressurize a material pipe (203), and then starting a lifting material cutting assembly (3) and a rotating receiving assembly (4) to enable the material in the material pipe (203) to enter a linear material feeding nozzle (310) and feed the material into a culture dish through the linear material feeding nozzle (310);

fourthly, when a plurality of culture dishes are arranged, the rotating bearing component (4) is started and drives the culture dishes to rotate, so that the culture dishes are sequentially fed by the linear feeding nozzles (310), after the culture dishes are sequentially fed by the linear feeding nozzles (310), the lifting blanking component (3) switches the next linear feeding nozzle (310) to feed the culture dishes again, and then the steps are repeated to switch materials, so that the culture dishes are fed by the materials according to the required culture medium condition information until the required culture medium condition information is met, and the culture dish feeding work is finished;

step five, after the feeding work of the culture medium materials is finished, starting the lifting and blanking assembly (3) and the rotating and receiving assembly (4) to work, controlling a linear feeding nozzle (310) to be inserted into the culture materials in the culture dish after the lifting and blanking assembly (3) works, then controlling the annular shell (301) to rotate in a reciprocating fixed angle by the rotating and receiving assembly (4), driving the culture dish to slightly return and deflect after the annular shell (301) rotates in the reciprocating fixed angle, enabling the culture materials in the culture dish to vibrate and slightly stir the culture materials in the culture dish through the linear feeding nozzle (310), and accordingly enabling the culture materials in the culture dish to be uniformly mixed;

sixthly, after culture materials in the culture dish are uniformly mixed, starting a heater (5) to perform heating sterilization treatment on the culture dish, emulsifying or galactizing the culture medium in the culture dish, then starting a vacuum machine (6) to extract gas generated by emulsification, then closing the vacuum machine (6) and the heater (5), then opening a one-way electromagnetic valve (105) to flush clean cold air, cooling the culture medium in the culture dish, simultaneously preventing the foreign gas outside the culture dish after opening a sealing split door (104) from rapidly entering a culture shell (1) to pollute the culture medium in the culture dish, and opening the sealing split door (104) and taking out the culture dish when the culture medium in the culture dish is cooled to normal temperature, performing refrigeration treatment on the culture dish containing the culture medium, thereby completing automatic and efficient preparation of the culture medium;

seventhly, after the large intestine group bacteria need to be cultured, taking out a culture dish containing a culture medium, dripping single large intestine group bacteria original strains, inserting the culture dish into an installation port (404) of the annular shell (301), extruding and fixing the culture dish by a first extruding and fixing arc sleeve (405) and a second extruding and fixing arc sleeve (406), starting a vacuum machine (6) to vacuumize the culture dish to remove miscellaneous gases, starting a heater (5) to keep the temperature of the annular shell (301) constant, opening a one-way electromagnetic valve (105) to input moisture into the annular shell, keeping the moisture in the culture shell (1) constant, ensuring that the temperature and the humidity of the equipment are most suitable for the existence of the single large intestine group bacteria original strains, controlling the lifting and cutting assembly (3) to switch corresponding materials when the culture medium materials in the culture dish are consumed or need to perform color reaction, and starting the receiving assembly (4) to drive the culture dish to be positioned at a rotary feeding nozzle (310) corresponding to the materials, then the material is put into a culture dish, and the material is filled, so that the rapid propagation of the single original strain of the large intestine group bacteria is ensured.

2. The large intestine group bacteria cultivation method based on the laboratory is characterized by comprising a cultivation shell (1), a pressure frame assembly (2), a lifting cutting assembly (3) and a rotating receiving assembly (4), wherein the pressure frame assembly (2) is fixedly arranged at the top of the cultivation shell (1), the lifting cutting assembly (3) is slidably arranged in the cultivation shell (1), the rotating receiving assembly (4) is rotatably arranged in the cultivation shell (1), the pressure frame assembly (2) and the lifting cutting assembly (3) are in through connection through a hose, the rotating receiving assembly (4) is arranged under the lifting cutting assembly (3), and the pressure frame assembly (2), the lifting cutting assembly (3) and the rotating receiving assembly (4) are located on the same central axis.

3. The culture apparatus of the large intestine group bacteria culture method based on the laboratory according to claim 2, wherein the pressure frame assembly (2) comprises a rectangular support (201), a pressurizing spring (202), a material pipe (203), a pressure cylinder (204), a pressure plate (206) and a pressure rod (205), the rectangular support (201) is fixedly arranged at the top end of the culture shell (1), the pressure plate (206) is slidably arranged in the rectangular support (201) and is abutted against the rectangular support, the pressurizing spring (202) is fixed at the bottom end of the pressure plate (206), a mounting snap ring (208) is installed at the bottom wall of the rectangular support (201), an I-shaped connecting pipe (207) is connected at the bottom end of the mounting snap ring (208) in a penetrating way, the pressure cylinder (204) is fixedly arranged at the center of the bottom wall of the rectangular support (201), and the top end of the pressure rod (205) is fixedly connected with the pressure plate (206), the other end of the material pipe is fixedly connected with a piston shaft of a pressure cylinder (204), the material pipe (203) is arranged between an installation clamping ring (208) and a pressurizing spring (202), and the pressurizing spring (202) is sleeved on the top end of the material pipe (203) and is abutted against the material pipe.

4. The laboratory-based cultivation apparatus for coliform bacteria culture method according to claim 3, wherein the pressure plate (206) is fixedly provided with sliding bolts (209) at both sides thereof, the rectangular bracket (201) is provided with slide ways (210) at both side walls thereof, the slide ways (210) are adapted to the sliding bolts (209), and the sliding bolts (209) slidably penetrate through the slide ways (210) of the rectangular bracket (201).

5. The cultivation apparatus for the laboratory-based large intestine flora culture method according to claim 3, wherein the material tube (203) comprises a tube body (2031), a piston ring (2032), a piston rod (2033), a T-shaped insertion protrusion (2034), and a discharge conduit (2035), the discharge conduit (2035) is fixedly arranged at the bottom end of the tube body (2031), the piston ring (2032) is slidably arranged in the tube body (2031) and is abutted against the inner wall of the piston ring (2032), the bottom end of the piston rod (2033) extends into the tube body (2031) through the top wall thereof and is fixedly connected with the piston ring (2032), the top end thereof is fixedly connected with the bottom end of the T-shaped insertion protrusion (2034), the top end of the T-shaped insertion protrusion (2034) is inserted into the pressurizing spring (202), the pressurizing spring (202) is sleeved on the top end of the T-shaped insertion protrusion (2034) and is abutted against the piston ring (2035), and the discharge conduit (2035) is clamped in the installation clamp ring (208), and an elastic cushion is arranged between the discharge conduit (2035) and the mounting snap ring (208).

6. The cultivation device of the laboratory-based coliform bacteria cultivation method according to claim 2, wherein the lifting and blanking assembly (3) comprises an annular housing (301), a vacuum machine (6), a heater (5), a one-way solenoid valve (105), an inner blanking ring plate (302), a lifting cylinder (306), a lifting rod (307), a first rotating rod (308) and a switching motor (309), the lifting cylinder (306) is fixedly arranged on the top wall of the cultivation shell (1), one end of the lifting rod (307) is fixedly connected with a piston shaft of the lifting cylinder (306), the other end of the lifting rod is fixed at the center of the top end of the annular housing (301), the switching motor (309) is fixedly arranged at the bottom end of the annular housing (301), the inner blanking ring plate (302) is rotatably arranged in the annular housing (301) and is abutted against the inner blanking ring plate, one end of the first rotating rod (308) is fixedly connected with the inner blanking ring plate (302), the other end of the switching motor penetrates through the inner wall of the annular shell (301) to extend to the outside of the annular shell and is fixedly connected with an output shaft of the switching motor (309);

the annular shell (301) is provided with a plurality of blank feeding holes (303) and a plurality of blank discharging holes (304), the blank feeding holes (303) are communicated with the I-shaped connecting pipe (207) through a hose, the blank feeding holes (303) and the blank discharging holes (304) are distributed in an annular array by taking the central axis of the annular shell (301) as the center of a circle, the blank feeding holes (303) and the blank discharging holes (304) are correspondingly arranged, the inner blank annular plate (302) is provided with a passage switching hole (305), and the passage switching hole (305) is movably communicated with the blank feeding holes (303) and the blank discharging holes (304) respectively;

blank discharge opening (304) through connection has linear feed nozzle (310), linear feed nozzle (310) are fixed to be located the bottom of annular shell (301), and linear feed nozzle (310) and blank discharge opening (304) are through corresponding quantity setting, and are a plurality of linear feed nozzle (310) encircle in the outside of switching motor (309), the outside symmetry of annular shell (301) is equipped with limit slide bar (312), limit slide bar (312) and cultivation casing (1) slide the setting, be equipped with sealed elastic ring (311) between annular shell (301) and interior blank crown plate (302), sealed elastic ring (311) offer the through-hole with blank discharge opening (304) adaptation.

7. The apparatus for cultivating the bacteria in the large intestine of the laboratory according to claim 2, wherein the rotating receiving assembly (4) comprises a rotating outer shell (401), a second rotating rod (402), a rotating motor (403), a supporting inner shell (413), a third rotating rod (411), a first extruding arc sleeve (405) and a second extruding arc sleeve (406), the rotating outer shell (401) is rotatably disposed on the cultivating shell (1), the rotating motor (403) is fixedly disposed at the bottom end of the cultivating shell (1), one end of the second rotating rod (402) is fixedly connected to the output shaft of the rotating motor (403), the other end of the second rotating rod is fixedly disposed at the center of the bottom of the rotating outer shell (401), the supporting inner shell (413) is fixedly disposed in the rotating outer shell (401), the top surface of the rotating outer shell (401) is provided with an installation opening (404) for placing the culture dish, the first extrusion arc sleeve (405) and the second extrusion arc sleeve (406) are arranged at the mounting opening (404) of the rotating shell (401), the first extrusion arc sleeve (405) and the second extrusion arc sleeve (406) are in clearance fit, and the first extrusion arc sleeve (405) is fixedly arranged in the rotating shell (401);

an extrusion fixing slide bar (407) is fixedly arranged on one side of the second extrusion fixing arc sleeve (406) opposite to the first extrusion fixing arc sleeve (405), an extrusion fixing spring (408) is sleeved at the outer end of the extrusion fixing slide bar (407), and one end of the extrusion fixing slide rod (407) far away from the second extrusion fixing arc sleeve (406) penetrates through the support inner shell (413) to extend to the interior of the support inner shell and is fixedly connected with a pull ring (409), the pull ring (409) is wound and fixed with a pull rope (410), the third rotating rod (411) is rotatably arranged at the center of the supporting inner shell (413), one end of the third rotating rod (411) is rotationally connected with the rotating shell (401) through a bearing, and the other end of the third rotating rod (411) penetrates through the top wall of the rotating shell (401) to extend to the outside of the rotating shell and is fixedly connected with a rotating handle (412), one end of the pull rope (410) far away from the pull ring (409) is wound at the outer end of the third rotating rod (411), and the pull rope (410) is fixedly connected with the third rotating rod (411).

8. The laboratory-based cultivation apparatus for coliform bacteria cultivation method according to claim 7, wherein the mounting opening (404), the first fixing and extruding arc sleeve (405), the second fixing and extruding arc sleeve (406), the fixing and extruding slide bar (407), the fixing and extruding spring (408), the pull ring (409) and the pull rope (410) are a set of self-locking pulling and disassembling structures, the number of self-locking pulling and disassembling structures is plural, and the self-locking pulling and disassembling structures are distributed in an annular array by taking a central point of the rotating housing (401) as a center.

9. The apparatus for culturing a large intestine group bacteria in a laboratory-based method according to claim 2, wherein said culture shell (1) is fixedly provided with a partition plate (101) and a heat insulation plate (102), and said culture shell (1) is hinged with a sealing split door (104).