CN215263248U - Test strip - Google Patents
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- CN215263248U CN215263248U CN202120553898.0U CN202120553898U CN215263248U CN 215263248 U CN215263248 U CN 215263248U CN 202120553898 U CN202120553898 U CN 202120553898U CN 215263248 U CN215263248 U CN 215263248U
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Abstract
The utility model relates to a detection test strip, which comprises a test strip body, wherein the test strip body is provided with a sample inlet, a first sample inlet channel and a second sample inlet channel which are communicated with the sample inlet; the first sample introduction channel is used for guiding a blood sample to a first working electrode, a first enzyme layer is coated on the first working electrode, and the first working electrode is used for detecting a blood creatinine signal and a creatine signal in the blood sample; the second sample introduction channel is used for guiding the blood sample to a second working electrode, a second enzyme layer is coated on the second working electrode, and the second working electrode is used for detecting a creatine signal in the blood sample. The detection test strip is simple in structure and convenient to use, can accurately detect the concentration of the blood creatinine, effectively eliminates the interference of endogenous creatine, and improves the accuracy of a test result.
Description
Technical Field
The utility model relates to a biological detection technical field especially relates to a test strip.
Background
With the development and progress of society, the living standard of people is gradually improved; meanwhile, along with the acceleration of life rhythm, the pressure born by people is increased, and the number of people in sub-health state is increased.
Creatinine (Cr), a polar organic nitrogenous compound with relatively small molecular mass (Mr-113.1188), is an end product of muscle creatine and phosphocreatine metabolism, and is an inner anhydrate formed from phosphocreatine by dephosphorylation and closure into a ring. The creatinine source in human body comprises two parts of exogenous source and endogenous source, and creatinine in blood almost completely enters into raw urine through glomerular filtration and is not reabsorbed by renal tubules; since the daily production of endogenous creatinine is almost constant, the concentration of blood creatinine is a stable value when the intake of exogenous creatinine is strictly controlled, and thus, the measurement of the concentration of blood creatinine can reflect the filtration function of glomeruli and is an important index for evaluating the filtration function of glomeruli.
At present, dry instruments and test strips are mostly adopted to detect the concentration of the blood creatinine, wherein the detection principle of the dry blood creatinine test strip is based on an enzyme method. However, the intermediate product in the reaction process of testing the blood creatinine by the enzyme method contains creatine, and because endogenous creatine exists in human bodies, the endogenous creatine can interfere with a test signal, so that the test result is inaccurate.
Therefore, how to provide a detection test strip which has a simple structure and is convenient to use, can accurately detect the concentration of the blood creatinine and effectively eliminate the interference of endogenous creatine becomes a technical problem to be solved by technical personnel in the field.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, an object of the utility model is to provide a detection test strip, this detection test strip simple structure, convenient to use can carry out accurate detection to blood creatinine concentration, effectively gets rid of endogenous creatine's interference, improves the accuracy of test result.
The technical scheme of the utility model as follows:
a detection test strip comprises a test strip body, wherein a sample inlet, a first sample inlet channel and a second sample inlet channel which are communicated with the sample inlet are arranged on the test strip body; the first sample introduction channel is used for guiding a blood sample to a first working electrode, a first enzyme layer is coated on the first working electrode, and the first working electrode is used for detecting a blood creatinine signal and a creatine signal in the blood sample; the second sample introduction channel is used for guiding the blood sample to a second working electrode, a second enzyme layer is coated on the second working electrode, and the second working electrode is used for detecting a creatine signal in the blood sample.
Preferably, the first working electrode is coated with a first enzyme layer, and the first enzyme layer is used for detecting blood creatinine and creatine in the blood sample; the second working electrode is coated with a second enzyme layer, and the second enzyme layer is used for detecting creatine in the blood sample.
Preferably, the test strip body comprises a substrate, the first working electrode and the second working electrode being disposed on the substrate; the surfaces of the first working electrode and the second working electrode are provided with insulating films, the insulating films are provided with openings, and the first enzyme layer and the second enzyme layer are arranged in the openings; a bonding layer and a hydrophilic film are sequentially arranged on one side, away from the substrate, of the insulating film, a sample guide groove is formed in the bonding layer, and the sample guide groove forms the sample inlet, the first sample introduction channel and the second sample introduction channel; be equipped with exhaust structure on the hydrophilic membrane, exhaust structure corresponds the setting and is in first kind of passageway the top of second kind of passageway.
Preferably, the sample guide groove is of an inverted U-shaped structure, a second opening is arranged at the top of the inverted U-shaped structure, the second opening forms the sample inlet, and two side arms of the inverted U-shaped structure respectively form the first sample inlet channel and the second sample inlet channel; the shape of the opening corresponds to the shape of the first sample feeding channel and the second sample feeding channel, and the first enzyme layer and the second enzyme layer are correspondingly arranged at two ends of the inverted U-shaped structure.
Preferably, the exhaust structure is an exhaust seam, and the exhaust seam is parallel to a connecting line of two ends of the inverted U-shaped structure.
Preferably, the sample guide groove is of an inverted Y-shaped structure, and the top of the inverted Y-shaped structure forms the sample inlet; the shape of the opening corresponds to that of the inverted Y-shaped structure; the first enzyme layer and the second enzyme layer are correspondingly arranged at two ends of the inverted Y-shaped structure.
Preferably, the exhaust structure is a first exhaust hole, and the first exhaust hole is correspondingly arranged at two ends of the inverted Y-shaped structure.
Preferably, the sample guide groove is of a shuttle-shaped structure, the sample inlet is positioned in the center of the shuttle-shaped structure, and the first sample inlet channel and the second sample inlet channel are respectively positioned on two sides of the sample inlet; the shape of the opening corresponds to the shape of the shuttle structure; the first enzyme layer and the second enzyme layer are correspondingly arranged at two ends of the fusiform structure.
Preferably, the hydrophilic membrane is provided with a sample injection port corresponding to the sample injection port, the exhaust structure is a second exhaust hole, and the second exhaust hole is correspondingly arranged at two ends of the shuttle-shaped structure.
Preferably, the side of the hydrophilic membrane far away from the adhesive layer is provided with a cover membrane.
Preferably, the substrate is a PET sheet.
The utility model provides a detection examination strip, this detection examination strip include the examination strip body, set up the introduction port on the examination strip body to and with communicating first introduction channel of introduction port and second introduction channel. When the blood sample detector is used, a blood sample to be detected is dripped into the sample inlet, and the blood sample flows into the first sample introduction channel and the second sample introduction channel respectively; wherein the first sample introduction channel is used for guiding the blood sample to a first working electrode coated with a first enzyme layer, and the first working electrode is used for detecting a blood creatinine signal and a creatine signal in the blood sample; the second sample introduction channel is used for guiding the blood sample to a second working electrode coated with a second enzyme layer, and the second working electrode is used for detecting a creatine signal in the blood sample. The utility model discloses a set up first kind passageway and second kind passageway to make first working electrode detect blood creatinine signal and creatine signal, second working electrode detects creatine signal, subtracts through the micro current signal that records two working electrodes, just can obtain accurate blood creatinine signal index, effectively avoids endogenous creatine's interference, has greatly improved the accuracy that detects the examination strip test.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural diagram of a test strip of the present invention;
FIG. 2 is a schematic diagram of a test strip of the present invention;
FIG. 3 is a schematic diagram of a test strip of the present invention;
reference numbers in the drawings illustrate: 1-sample inlet, 2-first sample introduction channel, 3-second sample introduction channel, 4-first working electrode, 41-first enzyme layer, 5-second working electrode, 51-second enzyme layer, 6-substrate, 7-insulating film, 71-opening, 8-bonding layer, 81-sample guide groove, 9-hydrophilic film and 91-exhaust structure.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the description of the present invention, it is to be understood that the terms "upper", "lower", and the like, indicate orientations or positional relationships, and are used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the components or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
As shown in fig. 1 to fig. 3, the present invention provides a test strip, which comprises a test strip body, wherein the test strip body is provided with a sample inlet 1, and a first sample inlet channel 2 and a second sample inlet channel 3 which are communicated with the sample inlet 1; the first sample introduction channel 2 is used for guiding a blood sample to the first working electrode 4, the first working electrode 4 is coated with a first enzyme layer 41, and the first working electrode 4 is used for detecting a blood creatinine signal and a creatine signal in the blood sample; the second sample feeding channel 3 is used for guiding the blood sample to the second working electrode 5, the second working electrode 5 is coated with the second enzyme layer 51, and the second working electrode 5 is used for detecting a creatine signal in the blood sample.
When the test strip provided by the utility model is used, a blood sample to be tested is dripped into the sample inlet 1, and the blood sample flows into the first sample inlet channel 2 and the second sample inlet channel 3 respectively; wherein the first sample channel 2 is used for guiding the blood sample to the first working electrode 4 coated with the first enzyme layer 41, and the first working electrode 4 is used for detecting a blood creatinine signal and a creatine signal in the blood sample; the second sample channel 3 is used to guide the blood sample to the second working electrode 5 coated with the second enzyme layer 51, and the second working electrode 5 is used to detect a creatine signal in the blood sample. Specifically, the blood sample itself contains a blood creatinine component, and also contains an endogenous creatine component; part of the blood sample entering the first sample feeding channel 2 reacts with enzyme under the action of the first enzyme layer 41 coated on the first working electrode 4, the intermediate product of the reaction of the blood creatinine and the enzyme comprises creatine, and the final intermediate product creatine reacts with the first enzyme layer 41 together with endogenous creatine, so that the signal obtained by the first electrode 4 comprises the signal obtained by the superposition of the blood creatinine and the endogenous creatine; the second enzyme layer 51 coated on the second working electrode 5 in the second sample injection channel 3 only reacts with the endogenous creatine in the blood sample and does not chemically react with the blood creatinine, so that the second electrode can directly detect the endogenous creatine signal in the blood sample. Thus, the signals of the two electrodes are subtracted, and the blood creatinine signal without the endogenous creatine can be obtained. The utility model discloses a set up first access way 2 and second access way 3 to make first working electrode 4 detect blood creatinine signal and creatine signal, second working electrode 5 detects the creatine signal, subtracts through the micro current signal that records two working electrodes, just can obtain accurate blood creatinine signal index, effectively avoids the interference of endogenous creatine, has greatly improved the accuracy that detects the examination strip test.
Preferably, the first working electrode 4 is coated with a first enzyme layer 41, and the first enzyme layer 41 is used for detecting blood creatinine and creatine in the blood sample; the second working electrode 5 is coated with a second enzyme layer 51, and the second enzyme layer 51 is used for detecting creatine in the blood sample.
Specifically, the first enzyme layer 41 contains creatininase, the second enzyme layer 51 does not contain creatininase, and the first enzyme layer 41 and the second enzyme layer 51 have the same other components. Thus, the first enzyme layer 41 may be used to detect blood creatinine and creatine in a blood sample, and the second enzyme layer 51 may detect creatine in a blood sample. By subtracting the two signals, the blood creatinine index in the blood sample can be obtained.
Preferably, the test strip body comprises a substrate 6, and the first working electrode 4 and the second working electrode 5 are arranged on the substrate 6; the surfaces of the first working electrode 4 and the second working electrode 5 are provided with an insulating film 7, the insulating film 7 is provided with an opening 71, and the first enzyme layer 41 and the second enzyme layer 51 are arranged in the opening 71; a bonding layer 8 and a hydrophilic film 9 are sequentially arranged on one side, away from the substrate 6, of the insulating film 7, a sample guide groove 81 is formed in the bonding layer 8, and the sample guide groove 81 forms a sample inlet 1, a first sample introduction channel 2 and a second sample introduction channel 3; be equipped with exhaust structure 91 on hydrophilic membrane 9, exhaust structure 91 corresponds the top of setting at first kind passageway 2, second kind passageway 3.
Among them, the adhesive layer 8 functions to adhere the hydrophilic film 9 to the insulating film 7. Specifically, the adhesive layer 8 may be a double-sided adhesive layer.
After entering the sample guide groove 81, the blood sample flows along the first sample channel 2 and the second sample channel 3 under the siphon action of the hydrophilic membrane 9, and contacts with the first enzyme layer 41 and the second enzyme layer 51 arranged in the opening 71 respectively. Since the insulating film 7 is provided with the opening 71, and the first enzyme layer 41 and the second enzyme layer 51 are disposed in the opening 71, the blood sample can be contacted with the first working electrode 4 and the second working electrode 5 through the first enzyme layer 41 and the second enzyme layer 51, respectively, to form micro-current, and then the micro-current is converted into a readable test value through the instrument.
The setting of exhaust structure 91 can regard as the gas outlet that the siphon was advanced kind, and at the in-process that the blood sample flows, with original air in first kind passageway 2/second kind passageway 3 through exhaust structure 91 discharge fast, reduces the resistance of advancing kind, guarantees the smooth and easy flow of liquid sample.
Preferably, the sample guide groove 81 is of an inverted U-shaped structure, a second opening 71 is arranged at the top of the inverted U-shaped structure, the second opening 71 forms the sample inlet 1, and two side arms of the inverted U-shaped structure respectively form the first sample inlet channel 2 and the second sample inlet channel 3; the shape of the opening 71 corresponds to the shape of the first sample channel 2 and the second sample channel 3, and the first enzyme layer 41 and the second enzyme layer 51 are correspondingly arranged at two ends of the inverted U-shaped structure.
Because the shape of the inverted U-shaped structure is round and smooth, the lines are smooth, the occurrence of the phenomenon of turbulent flow of the blood sample in the first sample introduction channel 2 and the second sample introduction channel 3 can be effectively reduced, the flow of the blood sample is mainly laminar flow, and the flow resistance of the blood sample is further reduced.
Preferably, the exhaust structure 91 is an exhaust slit, and the exhaust slit is parallel to a connecting line of two ends of the inverted U-shaped structure.
Preferably, the sample guide groove 81 is of an inverted Y-shaped structure, and a sample inlet 1 is formed at the top of the inverted Y-shaped structure; the shape of the opening 71 corresponds to the shape of the inverted-Y structure; the first enzyme layer 41 and the second enzyme layer 51 are disposed at both ends of the inverted-Y structure, respectively.
In a similar way, the transition between the main branch and the branch of the inverted Y-shaped structure is natural, the shape is smooth, and the lines are smooth, so that the occurrence of the turbulent phenomenon of the blood sample in the first sample feeding channel 2 and the second sample feeding channel 3 can be effectively reduced, the laminar flow is mainly used for the flow, and the flow resistance of the blood sample is further reduced.
Preferably, the exhaust structure 91 is a first exhaust hole, and the first exhaust hole is correspondingly disposed at two ends of the inverted Y-shaped structure.
Preferably, the sample guide groove 81 is of a shuttle-shaped structure, the sample inlet 1 is positioned in the center of the shuttle-shaped structure, and the first sample inlet channel 2 and the second sample inlet channel 3 are respectively positioned at two sides of the sample inlet 1; the shape of the opening 71 corresponds to the shape of the shuttle; the first enzyme layer 41 and the second enzyme layer 51 are correspondingly arranged at two ends of the fusiform structure.
Preferably, the hydrophilic membrane 9 is provided with a sample inlet corresponding to the position of the sample inlet 1, the air exhaust structure 91 is a second air exhaust hole, and the second air exhaust hole is correspondingly arranged at two ends of the shuttle-shaped structure.
To sum up, the embodiment of the utility model provides an in, the testing strip can adopt the side to advance kind and top advance kind dual mode, and the user of service can be accurate quick more find introduction port 1, advances kind conveniently.
Preferably, the side of the hydrophilic membrane 9 remote from the adhesive layer 8 is provided with a cover membrane.
Preferably, the substrate 6 is polyethylene terephthalate or polyvinyl chloride.
The embodiments in the present specification are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same or similar parts in the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A detection test strip is characterized by comprising a test strip body, wherein the test strip body is provided with a sample inlet, a first sample inlet channel and a second sample inlet channel which are communicated with the sample inlet;
the first sample introduction channel is used for guiding a blood sample to a first working electrode, a first enzyme layer is coated on the first working electrode, and the first working electrode is used for detecting a blood creatinine signal and a creatine signal in the blood sample;
the second sample introduction channel is used for guiding the blood sample to a second working electrode, a second enzyme layer is coated on the second working electrode, and the second working electrode is used for detecting a creatine signal in the blood sample.
2. The test strip of claim 1, wherein the first working electrode is coated with a first enzyme layer for detecting blood creatinine and creatine in the blood sample;
the second working electrode is coated with a second enzyme layer, and the second enzyme layer is used for detecting creatine in the blood sample.
3. The test strip of claim 2, wherein the strip body comprises a substrate, the first working electrode and the second working electrode being disposed on the substrate;
the surfaces of the first working electrode and the second working electrode are provided with insulating films, the insulating films are provided with openings, and the first enzyme layer and the second enzyme layer are arranged in the openings;
a bonding layer and a hydrophilic film are sequentially arranged on one side, away from the substrate, of the insulating film, a sample guide groove is formed in the bonding layer, and the sample guide groove forms the sample inlet, the first sample introduction channel and the second sample introduction channel;
be equipped with exhaust structure on the hydrophilic membrane, exhaust structure corresponds the setting and is in first kind of passageway the top of second kind of passageway.
4. The test strip of claim 3, wherein the sample guide groove is an inverted U-shaped structure, a top of the inverted U-shaped structure is provided with a second opening, the second opening forms the sample inlet, and two side arms of the inverted U-shaped structure form the first sample inlet channel and the second sample inlet channel respectively;
the shape of the opening corresponds to the shape of the first sample feeding channel and the second sample feeding channel,
the first enzyme layer and the second enzyme layer are correspondingly arranged at two ends of the inverted U-shaped structure.
5. The test strip of claim 4, wherein the vent structure is a vent slit parallel to a line connecting two ends of the inverted U-shaped structure.
6. The test strip of claim 3, wherein the sample guide groove is an inverted Y-shaped structure, and the top of the inverted Y-shaped structure forms the sample inlet;
the shape of the opening corresponds to that of the inverted Y-shaped structure;
the first enzyme layer and the second enzyme layer are correspondingly arranged at two ends of the inverted Y-shaped structure.
7. The test strip of claim 6, wherein the vent structure is a first vent hole, and the first vent hole is disposed at two ends of the inverted Y-shaped structure.
8. The test strip of claim 3, wherein the sample guide groove is a shuttle structure, the sample inlet is located at the center of the shuttle structure, and the first sample channel and the second sample channel are located at two sides of the sample inlet respectively;
the shape of the opening corresponds to the shape of the shuttle structure;
the first enzyme layer and the second enzyme layer are correspondingly arranged at two ends of the fusiform structure.
9. The test strip of claim 8, wherein the hydrophilic membrane is provided with a sample inlet corresponding to the sample inlet,
the exhaust structure is a second exhaust hole which is correspondingly arranged at two ends of the shuttle-shaped structure.
10. The test strip of claim 3, wherein the hydrophilic membrane is provided with a cover membrane on a side thereof remote from the adhesive layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120553898.0U CN215263248U (en) | 2021-03-17 | 2021-03-17 | Test strip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120553898.0U CN215263248U (en) | 2021-03-17 | 2021-03-17 | Test strip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215263248U true CN215263248U (en) | 2021-12-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120553898.0U Active CN215263248U (en) | 2021-03-17 | 2021-03-17 | Test strip |
Country Status (1)
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|---|---|
| CN (1) | CN215263248U (en) |
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2021
- 2021-03-17 CN CN202120553898.0U patent/CN215263248U/en active Active
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