CN219590235U - Blood glucose uric acid test paper with red blood cell packed volume correction function - Google Patents

Abstract

The utility model discloses blood glucose uric acid test paper with a red blood cell pressure accumulation correction function, which comprises a substrate, an electrode layer, an enzyme layer and a hydrophilic film layer which are arranged from bottom to top, wherein the enzyme layer comprises a uricase layer and a blood glucose enzyme layer, the electrode layer comprises a silver paste layer, a first carbon layer, a silver chloride electrode, a second carbon layer and an insulating layer, the first carbon layer comprises a blood glucose counter electrode and a blood glucose working electrode which form a current loop, the blood glucose enzyme layer covers the upper surfaces of the blood glucose counter electrode and the blood glucose working electrode, the second carbon layer comprises a uric acid working electrode, a first electrode and a second electrode, the uricase layer covers the upper surfaces of the uric acid working electrode and the silver chloride electrode which form a current loop, and a current loop is formed between the first electrode and the second electrode. The utility model can realize the detection of blood sugar and uric acid without mutual interference, and the test paper has the function of correcting the hematocrit, thereby improving the accuracy of the measurement results of blood sugar and uric acid.

Description

A blood glucose and uric acid test paper with hematocrit correction function

technical field

The utility model relates to a blood sugar and uric acid test paper, in particular to a blood sugar and uric acid test paper with a hematocrit correction function.

Background technique

Diabetes is one of the major metabolic diseases that endanger human health, and its symptoms are mainly manifested as abnormal glucose levels in blood and urine. At the same time, with the continuous improvement of living standards and the gradual changes in people's lifestyles, the number of people with high uric acid is increasing. Both high uric acid and high blood sugar are metabolic diseases and affect each other. According to research, for every 60 μmol/L increase in blood uric acid level, the risk of diabetes will increase by 18%. Therefore, detecting the content of blood sugar and uric acid in the blood is of great significance to the prevention and diagnosis of chronic diseases.

At present, single-function blood glucose test strips and uric acid test strips are relatively common in the market. Most of these test strips only have a single reaction zone and can only detect a single indicator. If you want to perform multi-component detection, you need to repeat the sample injection many times to achieve it, which brings a lot of inconvenience to the user during use and consumes a lot of test paper. The multi-functional test strip technology can perfectly solve the above problems, save the amount of blood collected, and simplify the operation steps. For example: Chinese utility model patent (CN 209086198 U) has announced a kind of blood sugar, uric acid dual-functional electrochemical test strip, by covering the first enzyme layer that reacts with uric acid and reacting with blood sugar in the first punching hole and the second punching hole The second enzyme layer realizes the simultaneous detection of blood sugar and uric acid, and the user does not need to change the device for each test, which is more convenient for the user.

In addition, for the determination of blood components such as glucose and uric acid concentration in the blood, since the test value changes with the hematocrit (hereinafter referred to as HCT), it will affect the accuracy of the final test results for low-volume or high-volume populations. Therefore, in order to obtain accurate and reliable detection results, it is necessary to perform HCT correction. For example, the Chinese invention patent (CN1059736946A) discloses a blood glucose test paper with hematocrit measurement and compensation, which dynamically calibrates the measured value of blood glucose concentration by measuring HCT, thereby improving the accuracy of the detection results. Chinese patent (CN 10911583 A) discloses a high-precision uric acid electrochemical test paper with hematocrit compensation, which uses the HCT influence curve on blood sugar concentration to compensate the uric acid measurement value, and the measurement accuracy range is changed from conventional 20%-50 % expanded to 10%-70%, expanding the scope of application of test paper.

In summary, the development of an electrochemical detection test strip with HCT correction function and the ability to simultaneously measure blood glucose and uric acid without interfering with each other is of great significance for improving the accuracy of test results and facilitating timely monitoring by users.

Contents of the invention

The technical problem to be solved by the utility model is to provide a blood glucose and uric acid test paper with hematocrit correction function in view of the deficiencies in the prior art. With the hematocrit (HCT) correction function, the blood glucose and uric acid measurement results are calibrated separately to improve the accuracy of the blood glucose and uric acid measurement results.

In order to solve the above-mentioned technical problems, the technical solution adopted by the utility model is: a blood glucose and uric acid test paper with hematocrit correction function, including a substrate, an electrode layer, an enzyme layer and a hydrophilic film layer arranged from bottom to top. The enzyme layer includes a uricase layer and a blood sugar enzyme layer, and the electrode layer includes a silver paste layer, a first carbon layer, a silver chloride electrode, a second carbon layer and an insulating layer, and the first carbon layer includes an electric current loop. The blood glucose counter electrode and the blood glucose working electrode, the blood glucose enzyme layer covers the upper surface of the blood glucose counter electrode and the blood glucose working electrode, the second carbon layer includes a uric acid working electrode, a first electrode and a second electrode, The uricase layer covers the upper surfaces of the uric acid working electrode and the silver chloride electrode forming a current loop, and a current loop is formed between the first electrode and the second electrode.

A current loop is formed between the blood glucose counter electrode and the blood glucose working electrode for measuring blood glucose. A current loop is formed between the uric acid working electrode and the silver chloride electrode for measuring uric acid. A current loop is formed between the first electrode and the second electrode for measuring the HCT impedance value.

In a preferred embodiment of the present invention, the second carbon layer is printed from carbon paste doped with oxidized electron mediators. The carbon slurry doped with the oxidized electron mediator can be the carbon slurry in the Chinese patent publication number CN102507670B, that is, the second carbon layer of the present application can be the working electrode 2 in the Chinese patent publication number CN102507670B.

In the utility model, the second carbon layer is set to include electron mediators, and the uricase layer does not contain electron mediators. The enzyme layer of the uricase layer does not contain electron mediators, and the remaining uric acid components will not interfere with the blood sugar circuit current through blood flow.

In a preferred embodiment of the present invention, the thickness of the carbon layer of the second carbon layer is 10um-300um.

In a preferred embodiment of the present invention, the electrode layer and the hydrophilic membrane layer are connected by a glue layer, and the glue layer is arranged around the enzyme layer.

In a preferred embodiment of the present invention, a siphon groove is formed between the top of the electrode layer, the glue layer, and the bottom of the hydrophilic film layer, and the uricase layer is close to the outside of the notch of the siphon groove The glucose enzyme layer is arranged away from the notch of the siphon groove, and the end of the hydrophilic film layer far away from the notch of the siphon groove is provided with a siphon hole.

The blood is sucked into the siphon groove by the siphon force of the siphon hole, so that the blood sugar and uric acid in the blood react with different enzyme layers to generate current, in which the uricase layer does not interfere with the blood sugar reaction current, and the blood glucose enzyme layer does not interfere with the uric acid reaction current.

The uricase layer is located at the front end of the test paper, and the enzyme layer does not contain electron mediators, and the remaining uric acid components will not interfere with the blood sugar circuit current through blood flow. The blood glucose enzyme layer is located on the innermost side of the test paper, and the electron mediator in the enzyme layer will not interfere with the uric acid circuit current along with the blood flow. Therefore, it is necessary to follow the order of the uricase layer at the front of the test paper and the blood glucose enzyme layer on the inside of the test paper to achieve a drop of blood. It can simultaneously measure two indicators of blood sugar and uric acid, and the test strip has the function of correcting hematocrit (HCT).

In a preferred embodiment of the present invention, the silver paste layer, the first carbon layer, the silver chloride electrode, the second carbon layer and the insulating layer are printed on the the upper surface of the substrate.

In a preferred embodiment of the present utility model, the adhesive layer is a double-sided adhesive layer.

In a preferred embodiment of the present invention, the substrate is a PET substrate.

Compared with the prior art, the utility model has the beneficial effects as follows:

① For the first time, one test strip and one drop of blood can be used to measure blood glucose and uric acid. At the same time, the test strip has the function of hematocrit (HCT) correction, which can calibrate the blood glucose and uric acid measurement results separately to improve the accuracy of the blood glucose and uric acid measurement results.

②The uric acid electrode is combined with the blood glucose electrode using doping technology, and a unique electrode structure layer is formed through 5 precise positioning printing, so that the uric acid measurement part does not interfere with the blood glucose measurement part, and the blood glucose measurement part does not interfere with the uric acid measurement part, achieving simultaneous measurement Blood sugar and uric acid can also ensure accuracy.

Description of drawings

Fig. 1 is the exploded view of the structure of the blood glucose and uric acid test paper of an embodiment of the present invention;

Fig. 2 is the electrode layer structural diagram of the blood glucose uric acid test paper of an embodiment of the present invention;

Fig. 3 is the front view of the blood glucose and uric acid test paper of an embodiment of the present invention;

Fig. 4 is a sectional view of the siphon groove of the blood glucose and uric acid test paper according to an embodiment of the present invention.

Among them, 1 substrate, 2 electrode layers, 21 silver paste layer, 22 first carbon layer, 23 silver chloride electrode, 24 second carbon layer, 25 insulating layer, 3 enzyme layer, 4 glue layer, 5 hydrophilic film layer, 6 siphon tank; 221 blood glucose counter electrode, 222 blood glucose working electrode, 241 uric acid working electrode, 242 first electrode, 243 second electrode, 31 uricase layer, 32 blood glucose enzyme layer.

Detailed ways

As shown in Figure 1, an embodiment of the present invention includes a PET substrate 1, an electrode layer 2, an enzyme layer 3 and a hydrophilic film layer 5 from bottom to top, the adhesive layer 4 is arranged around the enzyme layer 3, and the Adhesive layer 4 adopts double-sided adhesive layer. As shown in Figure 2, the electrode layer 2 is composed of a silver paste layer 21, a first carbon layer 22, a silver chloride electrode 23, a second carbon layer 24 and an insulating layer 25, and is printed on the PET substrate 1 sequentially from bottom to top. . As shown in Figures 3-4, the enzyme layer 3 is composed of a uricase layer 31 and a glucose enzyme layer 32, wherein the uricase layer 31 is close to the edge of the siphon groove 6, and the glucose enzyme layer 32 is located inside the siphon groove 6; The siphon groove 6 is formed by the PET substrate 1, the electrode layer 2, the double-sided adhesive tape 4 and the hydrophilic film 5. The blood is sucked into the siphon groove 6 by the siphon force of the siphon hole 51, so that the blood sugar and uric acid in the blood are separated from the different enzyme layers. The reaction generates a current, wherein the uricase layer 31 does not interfere with the blood glucose response current, and the glucose enzyme layer 32 does not interfere with the uric acid response current.

The first carbon layer 22 is composed of a blood glucose counter electrode 221 and a blood glucose working electrode 222 to form a current loop. The two electrodes are covered with a glucose enzyme layer 32 for measuring blood glucose.

The second carbon layer 24 is printed with a uric acid working electrode 241, a first electrode 242, and a second electrode 243, wherein the uric acid working electrode 241 and the silver chloride electrode 23 form a current loop for measuring uric acid, and the uricase layer 31 covers the uric acid working electrode. Between the electrode 241 and the silver chloride electrode 23; wherein the first electrode 242 and the second electrode 243 form a current loop for measuring the HCT impedance value. The second carbon layer 24 is printed from doped oxidized electron mediator carbon paste, the thickness of the carbon layer is controlled between 10um and 300um, and the oxidized electron mediator is ferrocene derivatives, tetrathiafulvalene One of TTF derivatives and methylene blue.

During work, the blood is sucked into the siphon tank 6, and the conductivity testing electrode composed of the first electrode 242 and the second electrode 243 measures the hematocrit HCT of the blood sucked into the siphon tank 6, and then the blood enters the uric acid electrochemical reaction successively. Section, blood glucose electrochemical reaction section, uric acid electrochemical reaction section includes uric acid working electrode 241 and silver chloride electrode 23 because the second carbon layer 24 is printed by doped oxidized electron mediator carbon paste, so in The uricase layer 31 no longer contains electron mediators, and no electron mediators will interfere with the blood sugar circuit current through the blood flow. The blood sugar electrochemical reaction part includes a blood sugar counter electrode 221 and a blood sugar working electrode 222 . Finally, use the HCT test value on the corresponding tester to calibrate the uric acid measurement value and blood glucose measurement value through the influence curve of HCT on uric acid concentration and blood sugar concentration, and finally record and display on the tester.

Claims (8)

1. Blood sugar uric acid test paper with red blood cell pressure accumulation correction function, characterized by including from bottom to top base plate (1), electrode layer (2), enzyme layer (3) and hydrophilic membrane layer (5) that set up, enzyme layer (3) include uricase layer (31) and blood sugar enzyme layer (32), electrode layer (2) include by silver thick liquid layer (21), first carbon layer (22), silver chloride electrode (23), second carbon layer (24) and insulating layer (25), first carbon layer (22) include blood sugar counter electrode (221) and blood sugar working electrode (222) that form the electric current loop, blood sugar enzyme layer (32) cover in blood sugar counter electrode (221) with the upper surface of blood sugar working electrode (222), second carbon layer (24) include uricase working electrode (241), first electrode (242) and second electrode (243), uricase layer (31) cover in form electric current loop uric acid working electrode (241) with silver chloride electrode (23) upper surface, between first electrode (242) and second electrode (243) form electric current loop.

2. The blood glucose uric acid test strip with a hematocrit correction function according to claim 1, characterized in that the second carbon layer (24) is printed with oxidized electron mediator doped carbon paste.

3. The blood glucose uric acid test strip with a hematocrit correction function according to claim 1, characterized in that a carbon layer thickness of the second carbon layer (24) is 10um to 300um.

4. A blood glucose uric acid test strip with a hematocrit correction function according to any of claims 1-3, characterized in that the electrode layer (2) and the hydrophilic membrane layer (5) are connected by a glue layer (4), the glue layer (4) being arranged around the enzyme layer (3).

5. The blood glucose uric acid test paper with the red blood cell pressure accumulation correction function according to claim 4, wherein a siphon groove (6) is formed between the upper side of the electrode layer (2), the lower side of the adhesive layer (4) and the lower side of the hydrophilic film layer (5), the uricase layer (31) is arranged close to the outer side of a notch of the siphon groove (6), the blood glucose enzyme layer (32) is arranged far away from the notch of the siphon groove (6), and a siphon hole (51) is formed in the end part of the hydrophilic film layer (5) far away from the notch of the siphon groove (6).

6. The blood glucose uric acid test strip with a hematocrit correction function according to any one of claims 1-3, characterized in that the silver paste layer (21), the first carbon layer (22), the silver chloride electrode (23), the second carbon layer (24) and the insulating layer (25) are printed on the upper surface of the substrate (1) in this order from bottom to top.

7. The blood glucose uric acid test strip with a hematocrit correction function as defined in claim 4, wherein the glue layer (4) is a double-sided glue layer.

8. The blood glucose uric acid test strip with a hematocrit correction function according to any one of claims 1-3, characterized in that the substrate (1) is a PET substrate.