CN111239229B - Dual-channel electrochemical biosensor and method for measuring heme concentration - Google Patents
Dual-channel electrochemical biosensor and method for measuring heme concentration Download PDFInfo
- Publication number
- CN111239229B CN111239229B CN202010110728.5A CN202010110728A CN111239229B CN 111239229 B CN111239229 B CN 111239229B CN 202010110728 A CN202010110728 A CN 202010110728A CN 111239229 B CN111239229 B CN 111239229B
- Authority
- CN
- China
- Prior art keywords
- reagent layer
- ferrocene
- electrode group
- channel
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/4163—Systems checking the operation of, or calibrating, the measuring apparatus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/27—Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
Abstract
The invention discloses a dual-channel electrochemical biosensor, which comprises a basal layer, wherein a first electrode group and a second electrode group are arranged at the lower end of the basal layer; the first electrode group and the second electrode group are provided with a counter electrode, a working electrode and an electrode output end; the substrate layer is provided with two reaction channels, the counter electrodes with fixed areas are exposed in the two reaction channels, and the working electrodes of the first electrode group and the second electrode group are respectively exposed; a hydrophilic layer is arranged on the reaction channel; the front end of the reaction channel is provided with a liquid inlet to be detected; the two reaction channels are respectively provided with a first reagent layer and a second reagent layer. The invention also discloses a method for measuring the heme concentration by using the dual-channel electrochemical biosensor.
Description
Technical Field
The invention belongs to the technical field of biosensing, and particularly relates to a dual-channel electrochemical biosensor and a method for measuring heme concentration.
Background
Biosensors are widely used in the fields of medical detection, environmental analysis, food detection, etc., and electrochemical biosensors are a technology for performing qualitative or quantitative analysis on an analyte by performing a chemical reaction with the analyte in a certain manner and converting the reaction signal into an electrical signal that can be analyzed and measured. The sensor has the characteristics of simple operation, small sample amount, high accuracy, low manufacturing cost, capability of being used for real-time detection and the like, but the conventional electrochemical biosensor is easily influenced by the hematocrit of blood when the concentration of an analyte in a blood sample is tested, so that the concentration result of the analyte is interfered. Most of the existing hematocrit test methods use a blood flow rate method, a conductance method or an impedance method to correct the analyte concentration. The methods are greatly influenced by the stability of a printing electrode of the sensor and the property of a hydrophilic layer film material, so that the development of a new detection method for improving the sensitivity and the accuracy of a detection means is a main problem to be solved urgently.
In an electrochemical biosensor, the level of hematocrit greatly biases the detection of the concentration of a component in blood, a higher level of hematocrit hinders the electron transfer between an electron enzyme mediator and a reactive component, the resulting signal value is lower, and the detection result is higher when the hematocrit is lower. At present, the red blood cell hematocrit correction technology is easily affected by an enzyme layer to blood components, so that a detection signal is unstable, the actual red blood cell hematocrit level of blood is difficult to accurately judge, and an accurate detection result cannot be obtained.
Disclosure of Invention
The invention provides a dual-channel electrochemical biosensor and a method for measuring the concentration of heme, which can be used for eliminating the influence of hematocrit on the measurement of the concentration of an analyte and improving the detection accuracy.
A double-channel electrochemical biosensor comprises a basal layer, wherein a first electrode group, a second electrode group and a first electrode group are arranged at the lower end of the basal layer, and the second electrode group is provided with a counter electrode, a working electrode and an electrode output end; the substrate layer is provided with two reaction channels, the counter electrodes with fixed areas are exposed in the two reaction channels, and the working electrodes of the first electrode group and the second electrode group are respectively exposed; a hydrophilic layer is arranged on the reaction channel; the front end of the reaction channel is provided with a liquid inlet to be detected, and the hydrophilic layers at the rear end of the reaction channel in the preferred mode can be respectively provided with air holes. A first reagent layer and a second reagent layer are respectively arranged on the two reaction channels; the first reagent layer comprises a hemolytic agent, a first electron mediator and a first stabilizing agent; the second reagent layer comprises a surfactant, a second electron mediator, an enzyme, and a second stabilizer.
Further, the buffer solution in the first reagent layer is used to maintain the pH value in the range of 6 to 8, and the buffer solution in the second reagent layer is used to maintain the pH value in the range of 5 to 8.
Preferably, the first electron mediator includes one of potassium ferricyanide, ferrocene, dimethylferrocene, and ferrocene dicarboxylate.
Preferably, the second electron mediator includes one of potassium ferricyanide, ruthenium hexaammonium trichloride, fulvalene tetrathio, ferrocene, dimethylferrocene, and ferrocene dicarboxylate.
Preferably, the hemolytic agent in the first reagent layer comprises one or more of saponin, dodecyl sulfate, cetyl trimethyl ammonium bromide and triton.
Preferably, the surfactant in the second reagent layer comprises one or more of saponin, dodecyl sulfuric acid, cetyl trimethyl ammonium bromide and triton.
The double-channel electrochemical biosensor with hematocrit correction comprises the following first electrode group and second electrode group: gold electrodes, carbon electrodes, silver electrodes or any combination thereof.
The invention also discloses a method for measuring the concentration of the heme by using the electrochemical biosensor.
The test conditions were: respectively applying the reaction solution to a first reaction channel and a second reaction channel under the constant potential condition of 0.2-1V for reaction; the buffer solution in the first reagent layer is used to maintain the pH in the range of 6 to 8, and the buffer solution in the second reagent layer is used to maintain the pH in the range of 5 to 8.
Preferably, the first electron mediator includes one of potassium ferricyanide, ferrocene, dimethylferrocene, and ferrocene dicarboxylate.
Preferably, the second electron mediator includes one of potassium ferricyanide, ruthenium hexaammonium trichloride, fulvalene tetrathio, ferrocene, dimethylferrocene, and ferrocene dicarboxylate.
Preferably, the hemolytic agent in the first reagent layer comprises one or more of saponin, dodecyl sulfuric acid, cetyl trimethyl ammonium bromide and triton.
Preferably, the surfactant in the second reagent layer comprises one or more of saponin, dodecyl sulfuric acid, cetyl trimethyl ammonium bromide and triton.
Compared with the prior art, the invention has the following technical scheme characteristics:
(1) The invention provides a brand-new electrochemical test strip structure which has multiple functions, small blood consumption, high blood inlet speed and convenient operation.
(2) The electrochemical test strip can be divided into two circuit structures and two reagent layer printing structures, and can meet the requirement of multipurpose test.
(3) The electrochemical test strip can save a large production cost and has good industrial application prospect.
Drawings
FIG. 1 is a schematic structural diagram of a dual-channel electrochemical biosensor according to an embodiment of the present invention.
Detailed Description
In order to make it possible for those skilled in the relevant art to understand the technical solutions of the present invention, the technical solutions in the embodiments of the present invention are completely and clearly described in conjunction with the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
FIG. 1 is a schematic structural diagram of the two-channel electrochemical biosensor with hematocrit correction according to the present embodiment, which includes a substrate layer 13, a conductive layer disposed on the substrate layer 13, and an insulating layer 11 disposed on the conductive layer. The upper end of the conducting layer comprises electrode output ends 1-5, the lower end of the conducting layer comprises a first working electrode 6, a second working electrode 7 and a counter electrode 8 shared by the first working electrode 6 and the second working electrode 7. Conductive traces 16 are connected between the first working electrode 6, the second working electrode 7, the counter electrode 8, and the electrode output terminals 1 to 5. The first working electrode 6 and the counter electrode 8 constitute a first electrode group, and the second working electrode 7 and the counter electrode 8 constitute a second electrode group. The insulating layer 11 covers the portion above the pins of the detection electrode group, and two inverted-Y-shaped reaction channels, namely a first channel 9 (first reagent layer) and a second channel 10 (second reagent layer), are arranged according to the arrangement of the electrodes of the conductive layer. A hydrophilic layer 12 is provided in the reaction channel. The front end of the hydrophilic layer 12 is provided with a liquid inlet 14 to be detected; the hydrophilic layers 12 at the rear ends of the reaction channels are respectively provided with air holes 15. In the two-channel electrochemical biosensor with hematocrit correction of the embodiment, the first electrode group and the second electrode group adopt silver and carbon electrodes.
The method for measuring the concentration of hemoglobin by using the electrochemical biosensor of the embodiment comprises the following test conditions: respectively applying the constant potential between 0.2 and 1 volt to the first reaction channel and the second reaction channel to carry out reaction; the buffer solution in the first reagent layer 9 is used to maintain the pH value in the range of 6 to 8, and the buffer solution in the second reagent layer 10 is used to maintain the pH value in the range of 5 to 8. The first reagent layer 9 includes a hemolytic agent, a first electron mediator, a first stabilizer; the second reagent layer 10 includes a surfactant, a second electron mediator, an enzyme, and a second stabilizer.
The first electron mediator comprises one of potassium ferricyanide, ferrocene, dimethylferrocene and ferrocene diformate. The second electron mediator comprises one of potassium ferricyanide, hexaammonium ruthenium trichloride, tetrathiafulvalene, ferrocene, dimethylferrocene and ferrocene diformate. The hemolytic agent in the first reagent layer comprises one or more of saponin, dodecyl sulfuric acid, hexadecyl trimethyl ammonium bromide and triton. The surfactant in the second reagent layer comprises one or more of saponin, dodecyl sulfuric acid, hexadecyl trimethyl ammonium bromide and triton.
The biosensor and method of the present embodiment can be used for hematocrit correction. Firstly, establishing a current correlation curve of different hematocrit values and the hemoglobin concentration in a blood sample, determining the hematocrit value of the blood sample according to the determined current and hematocrit correlation curve, correcting the analyte concentration by using the determined hematocrit value of the blood, eliminating the influence of the hematocrit value on the analyte concentration determination, and improving the accuracy of the analyte concentration test.
The above embodiments are merely illustrative of the technical solutions and features of the present invention, and are intended to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. Various changes or modifications of the invention based on the principles thereof are also intended to be defined by the following claims.
Claims (5)
1. A dual channel electrochemical biosensor, comprising: comprises a basal layer, wherein the lower end of the basal layer is provided with a first electrode group and a second electrode group; the first electrode group and the second electrode group are provided with a counter electrode, a working electrode and an electrode output end; the substrate layer is provided with two reaction channels, the counter electrodes with fixed areas are exposed in the two reaction channels, and the working electrodes of the first electrode group and the second electrode group are respectively exposed; a hydrophilic layer is arranged on the reaction channel; the front end of the reaction channel is provided with a liquid inlet to be detected; a first reagent layer and a second reagent layer are respectively arranged on the two reaction channels; the first reagent layer comprises a hemolytic agent, a first electron mediator and a first stabilizing agent; the second reagent layer comprises a surfactant, a second electron mediator, an enzyme and a second stabilizer;
the buffer solution in the first reagent layer is used for maintaining the pH value in the range of 6 to 8, and the buffer solution in the second reagent layer is used for maintaining the pH value in the range of 5 to 8;
the hemolytic agent in the first reagent layer comprises one or a combination of more of saponin, dodecyl sulfuric acid, hexadecyl trimethyl ammonium bromide and triton, and the surfactant in the second reagent layer comprises one or a combination of more of saponin, dodecyl sulfuric acid, hexadecyl trimethyl ammonium bromide and triton.
2. The dual-channel electrochemical biosensor of claim 1, wherein: the first electron mediator comprises one of potassium ferricyanide, ferrocene, dimethylferrocene and ferrocene diformate.
3. The dual-channel electrochemical biosensor of claim 1, wherein: the second electron mediator comprises one of potassium ferricyanide, hexaammonium ruthenium trichloride, tetrathiafulvalene, ferrocene, dimethylferrocene and ferrocene diformate.
4. A method for measuring hemoglobin concentration using the two-channel electrochemical biosensor of claim 1, wherein: respectively applying the constant potential between 0.2 and 1 volt to the first reaction channel and the second reaction channel to carry out reaction; the buffer solution in the first reagent layer is used for maintaining the pH value in the range of 6 to 8, and the buffer solution in the second reagent layer is used for maintaining the pH value in the range of 5 to 8;
the hemolytic agent in the first reagent layer comprises one or a combination of more of saponin, dodecyl sulfuric acid, hexadecyl trimethyl ammonium bromide and triton, and the surfactant in the second reagent layer comprises one or a combination of more of saponin, dodecyl sulfuric acid, hexadecyl trimethyl ammonium bromide and triton.
5. The method of measuring hemoglobin concentration of claim 4, wherein: the first reagent layer comprises a hemolytic agent, a first electron mediator and a first stabilizing agent; the second reagent layer comprises a surfactant, a second electron mediator, an enzyme and a second stabilizer;
the first electron mediator comprises one of potassium ferricyanide, ferrocene, dimethyl ferrocene and ferrocene diformate; the second electron mediator comprises one of potassium ferricyanide, hexaammonium ruthenium trichloride, tetrathiafulvalene, ferrocene, dimethylferrocene and ferrocene diformate.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010110728.5A CN111239229B (en) | 2020-02-24 | 2020-02-24 | Dual-channel electrochemical biosensor and method for measuring heme concentration |
| PCT/CN2020/114236 WO2021169242A1 (en) | 2020-02-24 | 2020-09-09 | Dual-channel electrochemical biosensor and method for measuring heme concentration |
| DE212020000534.6U DE212020000534U1 (en) | 2020-02-24 | 2020-09-09 | Electrochemical biosensor with two channels for measuring hemoglobin concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010110728.5A CN111239229B (en) | 2020-02-24 | 2020-02-24 | Dual-channel electrochemical biosensor and method for measuring heme concentration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111239229A CN111239229A (en) | 2020-06-05 |
| CN111239229B true CN111239229B (en) | 2023-04-11 |
Family
ID=70873127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010110728.5A Active CN111239229B (en) | 2020-02-24 | 2020-02-24 | Dual-channel electrochemical biosensor and method for measuring heme concentration |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN111239229B (en) |
| DE (1) | DE212020000534U1 (en) |
| WO (1) | WO2021169242A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111239229B (en) * | 2020-02-24 | 2023-04-11 | 江苏鱼跃医疗设备股份有限公司 | Dual-channel electrochemical biosensor and method for measuring heme concentration |
| CN115372428A (en) * | 2022-04-14 | 2022-11-22 | 深圳可孚生物科技有限公司 | Electrochemical sensor based on redundant electrode and attenuation compensation method thereof |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW466344B (en) * | 2000-09-01 | 2001-12-01 | Apex Biotechnology Corp | Disposable electrode for whole blood hemoglobin (HGB) and hematocrit (HCT) measurement, and preparation and application thereof |
| US7501053B2 (en) * | 2002-10-23 | 2009-03-10 | Abbott Laboratories | Biosensor having improved hematocrit and oxygen biases |
| WO2005012900A1 (en) * | 2003-06-20 | 2005-02-10 | Roche Diagnostics Gmbh | Biosensor with multiple electrical functionalities |
| EP3273232A2 (en) * | 2003-12-04 | 2018-01-24 | Panasonic Healthcare Holdings Co., Ltd. | Method of measuring blood component, sensor used in the method, and measuring device |
| US9012232B2 (en) * | 2005-07-15 | 2015-04-21 | Nipro Diagnostics, Inc. | Diagnostic strip coding system and related methods of use |
| CN1710418A (en) * | 2004-06-17 | 2005-12-21 | 中国科学院电子学研究所 | Biological sensor for quickly detecting hemoglobin and detecting method thereof |
| US20080083618A1 (en) * | 2006-09-05 | 2008-04-10 | Neel Gary T | System and Methods for Determining an Analyte Concentration Incorporating a Hematocrit Correction |
| CN101430336B (en) * | 2007-11-05 | 2012-12-19 | 勤立生物科技股份有限公司 | Method for hemachrome or hematocrit detection by electro-chemistry method, and detection test piece thereof |
| TWI401431B (en) * | 2008-06-25 | 2013-07-11 | Apex Biotechnology Corp | Hemoglobin-detecting electrode test strip and device comprising the same |
| CN101943673B (en) * | 2009-07-07 | 2016-05-25 | 北京怡成生物电子技术股份有限公司 | Electrochemica biological sensor and preparation method thereof and cell overstock the method for proofreading and correct |
| TWI440853B (en) * | 2009-12-14 | 2014-06-11 | Taidoc Technology Corp | Electrochemical biosensing test strip, biosensing meter, system and measuring method for analyte measurement incorporating a hematocrit correction |
| CN102128932A (en) * | 2010-01-15 | 2011-07-20 | 泰博科技股份有限公司 | Electrochemical bio-sensing test paper, biosensor device and analyte measuring system |
| TWI493186B (en) * | 2013-02-08 | 2015-07-21 | Hmd Biomedical Inc | Test strip, detecting device and detection method |
| US20140262833A1 (en) * | 2013-03-14 | 2014-09-18 | Magellan Diagnostics, Inc. | Sensor with a bifurcated flowpath |
| CN206876623U (en) * | 2017-05-08 | 2018-01-12 | 桂林优利特医疗电子有限公司 | A kind of Electrochemical blood glucose biology sensor with packed cell volume calibration function |
| CN109613078B (en) * | 2018-12-12 | 2024-03-15 | 广州万孚生物技术股份有限公司 | Anti-interference electrochemical detection sensor and manufacturing method thereof |
| CN110133078A (en) * | 2019-04-25 | 2019-08-16 | 广州万孚生物技术股份有限公司 | Glutamic-pyruvic transaminase Electrochemical Detection composition, its application, electrochemical sensor and detection method |
| CN110133079A (en) * | 2019-04-25 | 2019-08-16 | 广州万孚生物技术股份有限公司 | Glutamic-oxalacetic transaminease Electrochemical Detection composition, its application, electrochemical sensor and detection method |
| CN110133080B (en) * | 2019-04-25 | 2021-11-02 | 广州万孚生物技术股份有限公司 | Hemoglobin electrochemical detection composition and hemoglobin electrochemical sensor |
| CN111239229B (en) * | 2020-02-24 | 2023-04-11 | 江苏鱼跃医疗设备股份有限公司 | Dual-channel electrochemical biosensor and method for measuring heme concentration |
-
2020
- 2020-02-24 CN CN202010110728.5A patent/CN111239229B/en active Active
- 2020-09-09 WO PCT/CN2020/114236 patent/WO2021169242A1/en active Application Filing
- 2020-09-09 DE DE212020000534.6U patent/DE212020000534U1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| DE212020000534U1 (en) | 2021-11-19 |
| WO2021169242A1 (en) | 2021-09-02 |
| CN111239229A (en) | 2020-06-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111239227B (en) | Erythrocyte volume correction method and biosensor testing device | |
| CN102778487B (en) | Oxidizable species as an internal reference in control solutions for biosensors | |
| KR101332738B1 (en) | Analyte determination method and analyte meter | |
| Brett et al. | Poly (methylene blue) modified electrode sensor for haemoglobin | |
| US7771583B2 (en) | Electrochemical determination of analytes | |
| KR101005559B1 (en) | Protein measurement apparatus by using biosensor | |
| TWI525322B (en) | Method of measurement of sample to be analyzed | |
| US20100089774A1 (en) | Non-enzymatic electrochemical method for simultaneous determination of total hemoglobin and glycated hemoglobin | |
| WO2006109277A2 (en) | Determination of partial fill in electrochemical strips | |
| CN111239229B (en) | Dual-channel electrochemical biosensor and method for measuring heme concentration | |
| WO2020094082A1 (en) | Potentiometric biosensor and detection method | |
| KR101357134B1 (en) | Method for Measuring Analytes in Blood Samples Using Electrochemical Biosensor and a Portable Analyzer | |
| Wang et al. | All-solid-state blood calcium sensors based on screen-printed poly (3, 4-ethylenedioxythiophene) as the solid contact | |
| CN105784814A (en) | Sensor based on concentration cell principle | |
| US20140246335A1 (en) | Method of determining hematocrit value, quantative analysis method, and sensor chip | |
| Han et al. | Amperometric response of solid-contact ion-selective electrodes utilizing a two-compartment cell and a redox couple in solution | |
| CN111812175B (en) | Blood detection method for reducing interference of hematocrit and biosensor | |
| CN104535631A (en) | Electrochemical measurement method | |
| CN112229884A (en) | Vitamin detection printed electrode based on carbon paste modification process and preparation process thereof | |
| Mori et al. | Amperometric detection with microelectrodes in flow injection analysis: theoretical aspects and application in the determination of nitrite in saliva | |
| CN111239228A (en) | Electrochemical biosensor and method for measuring blood impedance phase angle | |
| Han et al. | Fast and sensitive coulometric signal transduction for ion-selective electrodes by utilizing a two-compartment cell | |
| Watanabe et al. | An electrochemical microfluidic device for non-enzymatic cholesterol determination using a lab-made disposable electrode | |
| CN210113571U (en) | Electrode test paper with impedance test function | |
| RU2696499C1 (en) | Biosensor for simultaneous glucose and blood lactate determination |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220111 Address after: 212300 Yunyang Industrial Park, Danyang City, Zhenjiang City, Jiangsu Province Applicant after: JIANGSU YUYUE MEDICAL EQUIPMENT&SUPPLY Co.,Ltd. Applicant after: Jiangsu Yuyue kailite Biotechnology Co.,Ltd. Address before: 212300 Yunyang Industrial Park, Danyang City, Zhenjiang City, Jiangsu Province Applicant before: JIANGSU YUYUE MEDICAL EQUIPMENT&SUPPLY Co.,Ltd. Applicant before: JIANGSU YUYUE INFORMATION TECHNOLOGY SYSTEM Co.,Ltd. Applicant before: SUZHOU YUYUE MEDICAL TECHNOLOGY Co.,Ltd. Applicant before: SUZHOU MEDICAL APPLIANCE FACTORY Applicant before: NANJING YUWELL SOFTWARE TECHNOLOGY Co.,Ltd. Applicant before: TIBET YUYUE MEDICAL INVESTMENT Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |