CN215066329U - Micro-fluidic sensing chip - Google Patents
Micro-fluidic sensing chip Download PDFInfo
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- CN215066329U CN215066329U CN202023230359.8U CN202023230359U CN215066329U CN 215066329 U CN215066329 U CN 215066329U CN 202023230359 U CN202023230359 U CN 202023230359U CN 215066329 U CN215066329 U CN 215066329U
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Abstract
The utility model relates to the technical field of clinical detection and analysis, and discloses a micro-fluidic sensing chip, which comprises an upper shell, a lower shell and a sealing plate arranged between the upper shell and the lower shell, wherein a runner groove is arranged in the cavity of the upper shell, the sealing plate and the upper shell are enclosed to form a runner, the runner comprises a main runner, a first subchannel and a second subchannel, the first subchannel and the second subchannel are respectively communicated with the main runner, and a sample adding port is arranged on the upper shell corresponding to the main runner, the micro-fluidic sensing chip of the embodiment of the utility model can divide a blood sample into the first subchannel and the second subchannel after being dripped into the main runner, a plate electrode can respectively detect the blood sample in the first subchannel and the blood sample in the second subchannel and simultaneously detect the charge characteristics of two types of blood, the detection efficiency is improved, and the operation is simple.
Description
Technical Field
The utility model relates to a clinical detection and analysis technical field especially relates to a micro-fluidic sensing chip.
Background
The phenomenon of blood coagulation dysfunction is more and more common in the current society, and the conventional blood coagulation function tests (PT, APTT, LIB and the like) are single blood coagulation indexes and cannot comprehensively reflect the blood coagulation process. For example, routine assays for coagulation do not reflect the interrelationship of platelets and coagulation cascades. Therefore, the thromboelastogram is widely applied to clinical examination, assists medical staff to judge the blood coagulation function state of a patient more comprehensively and effectively and takes corresponding treatment measures. The earliest thromboelastogram instruments all adopt a suspension wire method, have the defects of high operation requirement, low detection flux, large instrument volume, large required blood volume and the like, and the prior art also adopts a microfluidic chip for detection, so that the defects are overcome, but the existing microfluidic chip can only be used for detecting one blood charge characteristic once, and various blood charge characteristics are obtained through multiple detections, so that a thromboelastogram curve is obtained, the detection efficiency is low, and the operation is complicated.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing a micro-fluidic sensor chip to solve current micro-fluidic chip and once only can be used for the detection of a blood lotus electric property, detection efficiency is low, complex operation's technical problem.
In order to solve the technical problem, the utility model provides a micro-fluidic sensing chip, including epitheca, inferior valve with set up in the epitheca with closing plate between the inferior valve, the epitheca is opening cavity structures down, the intracavity of epitheca is provided with the runner groove, the closing plate with the epitheca encloses to close and forms the runner, the runner includes sprue, first subchannel and second subchannel, first subchannel with the second subchannel respectively with the sprue intercommunication, correspond on the epitheca the position of sprue is provided with the sample injection port, still including set up in the inferior valve with plate electrode between the closing plate, the plate electrode is used for right blood sample in the first subchannel with blood sample in the second subchannel detects respectively.
Optionally, the first sub-passage and the second sub-passage are symmetrically arranged with respect to a center line in the width direction of the upper case.
Optionally, an air pump interface is disposed on the upper case between the first sub-channel and the second sub-channel, and the air pump interface is used for externally connecting an air pump to enable the blood sample in the main flow channel to be shunted into the first sub-channel and the second sub-channel.
Optionally, the electrode plate has a first electrode pair and a second electrode pair, the sealing plate is provided with a first through hole and a second through hole, the first electrode is disposed below the first through hole, the second electrode pair is disposed below the second through hole, the first through hole is communicated with the first sub-channel, and the second through hole is communicated with the second sub-channel.
Optionally, a first contact and a second contact for externally connecting a detection device are further disposed on the electrode plate, the first electrode is connected to the first contact through a first wire, and the second electrode is connected to the second contact through a second wire.
Optionally, the sample injection device further comprises a top cover plate, the top cover plate is arranged at the upper end of the upper shell in a covering mode, the top cover plate is provided with a notch matched with the sample injection port, and the top cover plate is further provided with a plug hole communicated with the air pump interface.
Optionally, the sample injection device further comprises a cover cap, wherein the cover cap is arranged at the sample injection port and clamped at the notch.
Optionally, the cap is made of a silica gel material.
The embodiment of the utility model provides a micro-fluidic sensing chip compares with prior art, and its beneficial effect lies in:
the utility model discloses micro-fluidic sensing chip sets up the runner groove through the intracavity at the epitheca, and closing plate and epitheca enclose to close and form the runner, and the runner includes the sprue and rather than the first subchannel and the second subchannel of intercommunication, and blood sample can shunt first subchannel and second subchannel after dropwise add the sprue, and the plate electrode can be right blood sample in the first subchannel with blood sample in the second subchannel detects respectively, detects two kinds of blood charge characteristics simultaneously, has improved detection efficiency, and easy operation.
Drawings
Fig. 1 is a schematic structural diagram of a microfluidic sensor chip according to an embodiment of the present invention;
fig. 2 is an exploded schematic view of a microfluidic sensor chip according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a flow channel of a microfluidic sensor chip according to an embodiment of the present invention;
fig. 4 is a schematic circuit structure diagram of an electrode plate of a microfluidic sensor chip according to an embodiment of the present invention.
In the figure, 1, an upper shell, 2, a lower shell, 3, a sealing plate, 31, a first through hole, 32, a second through hole, 4, a flow channel, 41, a main flow channel, 42, a first sub-channel, 43, a second sub-channel, 5, a sample port, 6, an electrode plate, 61, a first electrode pair, 62, a second electrode pair, 63, a first contact, 64, a second contact, 7, an air pump interface, 8, a top cover plate, 9, a jack, 10 and a cap.
Detailed Description
It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.
In addition, the following description will be briefly made with reference to the orientation: the following directional or positional relationships, as indicated in the context of "front", "rear", "upper", "lower", "left", "right", etc., with respect to each structural member, are intended to refer to the orientation or positional relationship as shown in the drawings; these positional relationships are merely for convenience and simplicity of description and do not indicate or imply that the device referred to must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be construed as limiting the invention.
If there is a description in an embodiment of the present invention referring to "first", "second", etc., the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated.
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, embodiments accompanied with figures are described in detail below.
Blood consists of plasma and blood cells. The plasma is light yellow translucent liquid, and contains a large amount of water and inorganic salts, fibrinogen, albumin, enzymes, and various nutrients and metabolites. Blood cells are classified into erythrocytes, leukocytes, platelets, and the like. Various components in blood store human health information, and blood tests are required for many diseases.
In blood, various substances have different charge characteristics. For example, red blood cells generate negative charges due to the specific membrane structure on the surface, so that the red blood cells as a whole exhibit negative charge characteristics. Albumin also exhibits a negative charge characteristic in a weak base environment. While inorganic salts are generally metal ions, mostly of a positively charged nature. In normal blood, various positively and negatively charged substances are maintained in a relatively uniform distribution and in a relatively balanced state.
Thrombi are mainly composed of a lattice formed by insoluble fibrin, aggregated platelets, and red blood cells trapped in the lattice. Therefore, the thrombus component has a negative charge property as a whole.
At the onset of blood coagulation, the blood chargeability distribution is relatively uniform due to the relative effect of the charges. As blood clotting progresses, thrombus develops. In the process of thrombus formation, a thrombus part and a non-thrombus part are gradually separated, and further the charge distribution of blood is not uniform. This change in charge distribution necessarily results in a change in the charge characteristics during blood coagulation. The whole blood coagulation process is analyzed by a comprehensive algorithm through the real-time monitoring of the change of the charge characteristics, and then a thrombus elastogram curve is obtained.
As shown in fig. 1-4, the microfluidic sensor chip according to the preferred embodiment of the present invention includes an upper casing 1, a lower casing 2 and a sealing plate 3 disposed between the upper casing 1 and the lower casing 2, the upper casing 1 is a cavity structure with a downward opening, a channel groove is disposed in the cavity of the upper casing 1, the sealing plate 3 and the upper casing 1 enclose to form a channel 4, the channel 4 includes a main channel 41, a first sub-channel 42 and a second sub-channel 43, the first sub-channel 42 and the second sub-channel 43 are respectively communicated with the main channel 41, a sample addition port 5 is disposed at a position on the upper casing 1 corresponding to the main channel 41, the sample addition port is used for dropping a blood sample, the blood sample enters the main channel from the sample addition port, the microfluidic sensor chip further includes an electrode plate 6 disposed between the lower casing 2 and the sealing plate 3, the electrode plate 6 is used for comparing the blood sample in the first sub-channel 42 and the blood sample in the second sub-channel 43 respectively The detection is carried out, specifically, the electrode plate is electrically connected with an external detection device, the external detection device detects an electric signal generated on the electrode plate, a blood sample in the first subchannel can be used for detecting the resistance characteristic of the blood sample, a blood sample in the second subchannel can be used for detecting the capacitance characteristic of the blood sample, and the change curves of the two blood charge characteristic parameters are obtained through comprehensive analysis.
Based on above technical scheme, the closing plate is the silica gel closing plate, and the closing plate encloses with the inner chamber of epitheca and closes and form the runner, and blood can flow in the runner, and the silica gel closing plate is placed the epitheca with between the plate electrode, also can be used for strengthening the leakproofness between epitheca and the plate electrode, prevent the liquid seepage.
The first sub-channel 42 and the second sub-channel 43 are symmetrically arranged with respect to the center line of the upper case 1 in the width direction, so that the blood sample in the main flow channel can be uniformly distributed into the first sub-channel and the second sub-channel, thereby more accurately detecting the resistance and the capacitance of the blood sample respectively.
The epitheca 1 is last to be located first subchannel 42 with position between the second subchannel 43 is provided with air pump interface 7, air pump interface 7 is used for external air pump so that the blood sample of sprue 41 shunts in first subchannel 42 and the second subchannel 43, when the external air pump of air pump interface, the air pump work, the inner chamber of epitheca is negative pressure state, be convenient for blood sample fast shunt in the sprue to first subchannel and second in the subchannel, in this embodiment, the air pump interface sets up the position in the middle of first subchannel and second subchannel, the negative pressure can all have the distribution in first subchannel and second subchannel department like this, be favorable to flow equalizing in the sprue to first subchannel and second in the subchannel.
The electrode plate 6 is provided with a first electrode pair 61 and a second electrode pair 62, 2 groups of electrode pairs are arranged on the electrode plate and can be used for simultaneously detecting two blood electrical characteristics, the sealing plate 3 is provided with a first through hole 31 and a second through hole 32, the first electrode pair 61 is arranged below the first through hole 31, the second electrode pair 62 is arranged below the second through hole 32, the first through hole 31 is communicated with the first sub-channel 42, and the second through hole 32 is communicated with the second sub-channel 43, so that after a blood sample flows into the first sub-channel and the second sub-channel, the blood sample can flow out from the first through hole and the second through hole on the sealing plate and respectively contact with the first electrode pair and the second electrode pair on the electrode plate, and the resistance and capacitance characteristics of the blood sample are further measured.
Still be provided with first contact 63 and second contact 64 that are used for external check out test set on the electrode pad 6, first electrode pair 61 is connected through first wire first contact 63, second electrode pair 62 is connected through the second wire second contact 64, and the electrode pad is through the external check out test set of first contact and second contact, detects the analysis to the blood sample of first electrode pair and second electrode pair contact respectively.
The utility model provides a micro-fluidic sensing chip still includes lamina tecti 8, lamina tecti 8 lid is located the upper end of epitheca 1, lamina tecti 8 have with the breach of 5 adaptations of sample addition mouth, still be provided with on the lamina tecti 8 with the spliced eye 9 of air pump interface 7 intercommunication, preferably, be provided with the pellosil in the spliced eye, during the use, a stereoplasm air duct of connecting outside air pump directly pierces through the pellosil and inserts the air pump interface. The pellosil receives the extrusion of stereoplasm air duct, forms the sealing washer around the stereoplasm air duct, prevents gas leakage, and the lamina tecti is used for encapsulating the epitheca, gets into the dust in avoiding the epitheca, and the whole outward appearance of chip is more pleasing to the eye.
The utility model provides a micro-fluidic sensing chip still includes block 10, block 10 lid is located sample addition mouth 5 and joint in breach department, the cover is used for the cover protection of sample addition mouth, prevents that the chip is inside to receive the pollution.
The cap 10 is made of silica gel, and the cap has elasticity and is convenient to be in tight fit with the sample adding port.
Firstly, removing a silica gel cap at a sample adding port, and adding a certain amount of anticoagulation blood into the sample adding port; and then the micro-fluidic sensing chip is placed into an external detection device, and the external detection device inserts the hard air duct connected with the air pump into the air pump interface and simultaneously connects the detection device with the contact of the electrode plate. By controlling the air pump, the blood at the sample adding port flows to the first subchannel and the second subchannel along the main channel and finally flows to the first through hole and the second through hole. And obtaining a change curve of equivalent resistance and equivalent capacitance generated by the change of the charge distribution uniformity in the blood coagulation process through detection equipment.
To sum up, the embodiment of the utility model provides a micro-fluidic sensing chip compares with prior art, and its beneficial effect lies in: the utility model discloses micro-fluidic sensing chip sets up the runner groove through the intracavity at the epitheca, and closing plate and epitheca enclose to close and form the runner, and the runner includes the sprue and rather than the first subchannel and the second subchannel of intercommunication, and blood sample can shunt first subchannel and second subchannel after dropwise add the sprue, and the plate electrode can be right blood sample in the first subchannel with blood sample in the second subchannel detects respectively, can detect two kinds of blood charge characteristics simultaneously, has improved detection efficiency, and easy operation.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and replacements can be made without departing from the technical principle of the present invention, and these modifications and replacements should also be regarded as the protection scope of the present invention.
Claims (8)
1. The microfluidic sensing chip is characterized by comprising an upper shell (1), a lower shell (2) and a sealing plate (3) arranged between the upper shell (1) and the lower shell (2), wherein the upper shell (1) is of a cavity structure with a downward opening, a flow channel groove is formed in a cavity of the upper shell (1), the sealing plate (3) and the upper shell (1) are enclosed to form a flow channel (4), the flow channel (4) comprises a main flow channel (41), a first sub-channel (42) and a second sub-channel (43), the first sub-channel (42) and the second sub-channel (43) are respectively communicated with the main flow channel (41), a sample adding port (5) is arranged at a position, corresponding to the main flow channel (41), on the upper shell (1), and an electrode plate (6) arranged between the lower shell (2) and the sealing plate (3), and the electrode plate (6) is used for comparing a blood sample in the first sub-channel (42) with a blood sample in the second sub-channel (43) The blood samples were tested separately.
2. The microfluidic sensor chip according to claim 1, wherein the first sub-channel (42) and the second sub-channel (43) are symmetrically arranged with respect to a center line of the upper case (1) in a width direction.
3. The microfluidic sensor chip according to claim 1, wherein an air pump interface (7) is disposed on the upper case (1) between the first sub-channel (42) and the second sub-channel (43), and the air pump interface (7) is used for externally connecting an air pump to enable the blood sample in the main channel (41) to be divided into the first sub-channel (42) and the second sub-channel (43).
4. The microfluidic sensor chip according to claim 3, further comprising a top cover plate (8), wherein the top cover plate (8) is disposed on the upper end of the upper case (1), the top cover plate (8) has a notch adapted to the sample addition port (5), and the top cover plate (8) is further provided with a plug hole (9) communicated with the air pump interface (7).
5. The microfluidic sensor chip according to claim 4, further comprising a cap (10), wherein the cap (10) is disposed on the sample addition port (5) and is clamped in the gap.
6. The microfluidic sensor chip according to claim 5, wherein the cap (10) is made of a silicone material.
7. The microfluidic sensor chip according to claim 1, wherein the electrode plate (6) has a first electrode pair (61) and a second electrode pair (62), the sealing plate (3) has a first through hole (31) and a second through hole (32), the first electrode pair (61) is disposed below the first through hole (31), the second electrode pair (62) is disposed below the second through hole (32), the first through hole (31) communicates with the first sub-channel (42), and the second through hole (32) communicates with the second sub-channel (43).
8. The microfluidic sensor chip according to claim 7, wherein the electrode plate (6) is further provided with a first contact (63) and a second contact (64) for externally connecting a detection device, the first electrode pair (61) is connected to the first contact (63) through a first lead, and the second electrode pair (62) is connected to the second contact (64) through a second lead.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202023230359.8U CN215066329U (en) | 2020-12-28 | 2020-12-28 | Micro-fluidic sensing chip |
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| CN202023230359.8U CN215066329U (en) | 2020-12-28 | 2020-12-28 | Micro-fluidic sensing chip |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114371209A (en) * | 2022-01-13 | 2022-04-19 | 南京岚煜生物科技有限公司 | ACT detection chip |
| CN114558629A (en) * | 2022-03-03 | 2022-05-31 | 四川微康朴澜医疗科技有限责任公司 | Microfluidic thrombus elasticity analysis and detection kit |
| EP4235162A4 (en) * | 2022-01-13 | 2023-11-01 | Lansion Biotechnology Co., Ltd. | Electrochemical test base and device for activated clotting time |
| WO2024027667A1 (en) * | 2022-07-31 | 2024-02-08 | 深圳市理邦精密仪器股份有限公司 | Detection assembly for medical detection equipment, and medical detection equipment |
| CN114558629B (en) * | 2022-03-03 | 2024-06-04 | 四川微康朴澜医疗科技有限责任公司 | Microfluidic type thrombus elasticity analysis and detection kit |
-
2020
- 2020-12-28 CN CN202023230359.8U patent/CN215066329U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114371209A (en) * | 2022-01-13 | 2022-04-19 | 南京岚煜生物科技有限公司 | ACT detection chip |
| WO2023134167A1 (en) * | 2022-01-13 | 2023-07-20 | 南京岚煜生物科技有限公司 | Act test chip |
| EP4235162A4 (en) * | 2022-01-13 | 2023-11-01 | Lansion Biotechnology Co., Ltd. | Electrochemical test base and device for activated clotting time |
| CN114558629A (en) * | 2022-03-03 | 2022-05-31 | 四川微康朴澜医疗科技有限责任公司 | Microfluidic thrombus elasticity analysis and detection kit |
| CN114558629B (en) * | 2022-03-03 | 2024-06-04 | 四川微康朴澜医疗科技有限责任公司 | Microfluidic type thrombus elasticity analysis and detection kit |
| WO2024027667A1 (en) * | 2022-07-31 | 2024-02-08 | 深圳市理邦精密仪器股份有限公司 | Detection assembly for medical detection equipment, and medical detection equipment |
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