CN215162823U - Carrier and bioreactor for culturing cells - Google Patents

Abstract

The utility model provides a carrier and bioreactor for cultivating cells, the carrier includes: the carrier plate is provided with a flow guide hole penetrating along the thickness direction of the carrier plate, and the surface of at least one side of the carrier plate is provided with a plurality of grooves and/or a plurality of protrusions. In the application process, the carrier can be placed in the culture solution, the flow of the culture solution can be promoted through the flow guide holes, the surface area can be increased through the grooves and/or the protrusions, the growth space of the cells is enlarged, the cells can be attached to the carrier and continuously grow, the growth of the cells is facilitated, the digestion and amplification operation is simple, and the cells are conveniently digested and amplified step by step.

Description

Carrier and bioreactor for culturing cells

Technical Field

The utility model relates to the technical field of biology, concretely relates to a carrier and bioreactor for cultivating cells.

Background

In the process of cell culture, adherent cells need to be attached to a carrier for growth, and the carrier for adherent cell culture needs to meet the requirements of carrier surface area, cell density, cell activity and the like. The existing carrier cell culture has poor effect, complicated digestion and amplification operation procedures and no capability of digesting cells and amplifying the cells step by step.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model provides a carrier and bioreactor for culturing cell for solve the current carrier cell culture's the not good effect, the difficult cell of digestion and enlarge the problem step by step.

In order to solve the technical problem, the utility model discloses a following technical scheme:

in a first aspect, the present invention provides a carrier for culturing cells, comprising:

the carrier plate is provided with a flow guide hole penetrating along the thickness direction of the carrier plate, and the surface of at least one side of the carrier plate is provided with a plurality of grooves and/or a plurality of protrusions.

Wherein, one side of the carrier plate is provided with a plurality of grooves, and the other side of the carrier plate is provided with a plurality of protrusions.

Wherein, one side of the carrier plate is provided with a plurality of uniformly distributed grooves, and the other side of the carrier plate is provided with a plurality of uniformly distributed protrusions.

The side walls of the grooves are provided with communicating holes, and every two adjacent grooves are communicated through the communicating holes.

Wherein, the bottom wall of the groove is provided with an opening.

The carrier plate is circular, and the flow guide holes extend along the radial direction of the carrier plate.

The carrier plate is provided with a plurality of flow guide holes, and the flow guide holes are distributed along the circumferential direction of the mounting holes at intervals.

The carrier plates are arranged at intervals along a direction perpendicular to the carrier plates.

In a second aspect, the present invention provides a bioreactor comprising:

the carrier described in the above embodiments;

the jar body, the jar has feed inlet and discharge gate on the body, the carrier set up in the jar body.

Wherein, still include:

the stirrer comprises a stirring shaft and stirring blades, wherein the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body, and the stirring blades are positioned in the tank body.

The utility model discloses an above-mentioned technical scheme's beneficial effect as follows:

according to the utility model discloses a carrier for cultivating cell, include: the carrier plate is provided with a flow guide hole penetrating along the thickness direction of the carrier plate, and the surface of at least one side of the carrier plate is provided with a plurality of grooves and/or a plurality of protrusions. In the application process, the carrier can be placed in the culture solution, the flow of the culture solution can be promoted through the flow guide holes, the surface area can be increased through the grooves and/or the protrusions, the growth space of the cells is enlarged, the cells can be attached to the carrier and continuously grow, the growth of the cells is facilitated, the digestion and amplification operation is simple, and the cells are conveniently digested and amplified step by step.

Drawings

Fig. 1 is a schematic structural diagram of a carrier according to an embodiment of the present invention;

fig. 2 is another schematic structural diagram of a carrier according to an embodiment of the present invention;

fig. 3 is a side view of a carrier according to an embodiment of the present invention;

FIG. 4 is a schematic view of the structure of the bioreactor.

Reference numerals

A carrier plate 10; a groove 11; a protrusion 12;

a diversion hole 13; a mounting hole 14;

a tank 20; a stirring shaft 21; the stirring blade 22.

Detailed Description

For further understanding of the present invention, preferred embodiments of the present invention will be described below with reference to examples, but it should be understood that these descriptions are only for the purpose of further illustrating the features and advantages of the present invention, and are not intended to limit the present invention.

As shown in fig. 1 to 3, the present invention provides a carrier for culturing cells, comprising: at least one carrier plate 10, the carrier plate 10 may have one or more, which may be selected according to practical situations, for example, the carrier plate 10 may have a plurality, and a plurality of carrier plates 10 may be arranged at intervals. The carrier plate 10 is provided with a plurality of flow guide holes 13 penetrating through the thickness direction of the carrier plate 10, and the flow guide holes 13 may be circular, oval or the like, so as to facilitate the uniform flow of the culture solution. At least one side surface of the carrier plate 10 is provided with a plurality of grooves 11 and/or a plurality of protrusions 12, the grooves 11 may be round and long, and the protrusions 12 may be cylindrical or spherical. For example, at least one side surface of the carrier plate 10 is provided with a plurality of grooves 11, at least one side surface of the carrier plate 10 is provided with a plurality of protrusions 12, the protrusions 12 may be in a cylindrical shape, such as a cylinder, and a plurality of protruding stripes or particles may be further disposed on the surface of the cylinder to increase the surface area. The surface area can be increased by the plurality of grooves 11 and/or the plurality of protrusions 12, and the growth space of the cells can be increased. The carrier plate 10 may be a transparent material plate, and the protrusions 12 may also be made of a transparent material for easy observation.

In the application process, the carrier can be placed in the culture solution, the flow of the culture solution can be promoted through the flow guide holes 13, the surface area can be increased through the grooves 11 and/or the protrusions 12, the growth space of the cells is enlarged, the cells can be attached to the carrier and continuously grow, the growth of the cells is facilitated, the digestion and amplification operation is simple, and the cells are conveniently digested and amplified step by step.

In some embodiments, one side of the carrier plate 10 may be provided with a plurality of recesses 11 and the other side of the carrier plate 10 may be provided with a plurality of protrusions 12. Optionally, one side of the carrier plate 10 may be provided with a plurality of uniformly distributed grooves 11, and the other side of the carrier plate 10 may be provided with a plurality of uniformly distributed protrusions 12, which is beneficial for uniform growth of cells.

In other embodiments, the side walls of the grooves 11 may be provided with communication holes, and two adjacent grooves 11 are communicated through the communication holes, so that the flow of the culture solution between two adjacent grooves 11 is facilitated, the culture solution flows uniformly, and cells grow uniformly.

Optionally, the bottom wall of the groove 11 may be provided with an opening, through which the culture solution can flow on both sides of the carrier plate 10, so that the culture solution flows uniformly, which is beneficial for uniform growth of cells.

In some embodiments, the carrier plate 10 may be circular, the flow guiding holes 13 extend in the radial direction of the carrier plate 10, and the flow guiding holes 13 may be elongated, such as oval, so that the culture fluid flows uniformly in the radial direction of the carrier plate 10.

In the embodiment of the present invention, as shown in fig. 1 and fig. 2, a mounting hole 14 may be provided on the carrier plate 10, the mounting hole 14 may be circular, the mounting hole 14 is located in the middle of the carrier plate 10, and a plurality of flow guiding holes 13, for example, six or eight flow guiding holes, may be provided on the carrier plate 10, and the plurality of flow guiding holes 13 may be distributed along the circumferential interval of the mounting hole 14, so that the culture solution may flow uniformly.

In the embodiment of the present invention, the support plate 10 has a plurality of, and a plurality of support plates 10 can be arranged along the direction interval of the perpendicular to support plate 10, which can increase the surface area, and is favorable for the culture solution to flow between two adjacent support plates 10, so that the culture solution can flow uniformly.

The embodiment of the utility model provides a bioreactor, include:

the carrier and the tank body 20 in the above embodiment, the tank body 20 is provided with a feeding hole and a discharging hole, the carrier is arranged in the tank body 20, a plurality of carriers can be arranged in the tank body 20, and the plurality of carriers can be arranged at intervals, so as to facilitate the flowing of the culture solution.

In some embodiments, as shown in fig. 4, the bioreactor further comprises: the agitator, the agitator includes (mixing) shaft 21 and stirring paddle 22, and stirring paddle 22 establishes on (mixing) shaft 21, and the one end of (mixing) shaft 21 stretches into jar body 20, and the mounting hole 14 on support plate 10 can be passed to the one end of (mixing) shaft 21, and stirring paddle 22 is located jar body 20, and stirring paddle 22 can establish the one end at (mixing) shaft 21, can promote the culture solution uniform flow through stirring paddle 22, is favorable to the even growth of cell.

Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by those of ordinary skill in the art to which the invention belongs. The use of "first," "second," and similar terms in the description herein do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships are changed accordingly.

The foregoing is a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. A carrier for culturing cells, comprising:

the carrier plate is provided with a flow guide hole penetrating along the thickness direction of the carrier plate, and the surface of at least one side of the carrier plate is provided with a plurality of grooves and/or a plurality of protrusions.

2. The carrier of claim 1 wherein one side of said carrier plate is provided with a plurality of said recesses and the other side of said carrier plate is provided with a plurality of said protrusions.

3. The carrier as claimed in claim 2, wherein one side of the carrier plate is provided with a plurality of uniformly distributed recesses and the other side of the carrier plate is provided with a plurality of uniformly distributed protrusions.

4. The carrier as claimed in claim 1, wherein the side walls of the grooves are provided with communication holes, and adjacent two of the grooves are communicated through the communication holes.

5. The carrier of claim 1 wherein the bottom wall of the recess is provided with an opening.

6. The carrier of claim 1 wherein said carrier plate is circular and said flow-directing holes extend in a radial direction of said carrier plate.

7. The carrier of claim 1, wherein the carrier has a mounting hole located at a middle portion of the carrier, and the carrier has a plurality of flow-guiding holes spaced along a circumferential direction of the mounting hole.

8. The carrier of claim 1, wherein the carrier plate has a plurality of carrier plates, and the plurality of carrier plates are arranged at intervals along a direction perpendicular to the carrier plates.

9. A bioreactor, comprising:

the vector of any one of claims 1-8;

the jar body, the jar has feed inlet and discharge gate on the body, the carrier set up in the jar body.

10. The bioreactor of claim 9, further comprising:

the stirrer comprises a stirring shaft and stirring blades, wherein the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body, and the stirring blades are positioned in the tank body.

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