CN216838000U - Fixed bed bioreactor - Google Patents
Fixed bed bioreactor Download PDFInfo
- Publication number
- CN216838000U CN216838000U CN202121441817.4U CN202121441817U CN216838000U CN 216838000 U CN216838000 U CN 216838000U CN 202121441817 U CN202121441817 U CN 202121441817U CN 216838000 U CN216838000 U CN 216838000U
- Authority
- CN
- China
- Prior art keywords
- disc
- fixed bed
- bed bioreactor
- tank body
- carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a fixed bed bioreactor, fixed bed bioreactor includes: the device comprises a tank body, a centrifugal stirrer, a top cover and a disc-type carrier; the disc type carrier is arranged in the tank body, a through hole is formed in the circle center of the disc type carrier, and one end of a stirring shaft of the centrifugal stirrer penetrates through the through hole of the disc type carrier. The utility model provides a disc carrier direct mount can the internal space of the jar body at the inside of the jar body, just the disc carrier can not pile up together, and the resistance is less when consequently the nutrient solution flows, can guarantee that the velocity of flow of nutrient solution is fixed, the flow direction is unanimous and can not form the sinuous flow to guarantee that nutrient substance distributes evenly, and then can guarantee that the cell growth state of load on disc carrier is unanimous. In addition, when the cells are digested, the pancreatin can uniformly flow through the disc-shaped carriers along with the nutrient solution, so that the cells are conveniently separated from the disc-shaped carriers.
Description
Technical Field
The utility model relates to a bioreactor technical field, concretely relates to fixed bed bioreactor.
Background
According to the cell characteristics, adherent cells and suspension cells are generally divided, wherein the adherent cells need to be attached to a carrier for growth, and the suspension cells can independently grow. The carriers for adherent cell culture are generally classified into spherical carriers and sheet carriers. In order to meet the requirements of carrier surface area, cell density, cell activity, etc., the spherical carrier is generally 100-120 μm small spheres, and the sheet-shaped carrier is generally a strip-shaped or round paper sheet.
In the existing fixed bed bioreactor, a fixed hanging basket is formed by two mesh baffles and a tank body, a flaky carrier is arranged in the fixed hanging basket, adherent cells and a culture medium flow through the fixed hanging basket through a bioreactor stirring system, the adherent cells are adsorbed on the flaky carrier in the fixed hanging basket due to the adherent capacity of the adherent cells, and the culture medium flows through the flaky carrier through the bioreactor stirring system and provides nutrition for the cells, so that the growth and proliferation of the cells are maintained. However, in the prior art, the sheet-shaped carriers are limited in arrangement in the fixed hanging basket, and can only be stacked together in multiple layers, and the sheet-shaped carriers are tightly extruded, so that the resistance of cells to be cultured and a culture medium is large when the cells to be cultured and the culture medium flow through the sheet-shaped carriers, the flow path is short, and the cells and the culture medium are difficult to be uniformly distributed inside and outside. Meanwhile, because adherent cells are adsorbed on the closely stacked flaky carriers, the cells are difficult to separate from the carriers during digestion. In addition, the conventional fixed-bed bioreactor has the problems that the carrier loading is inconvenient and the cells are difficult to re-expand. The common defects of the spherical carrier and the flaky carrier are higher cost, poor repeated use effect and complex digestion and amplification operation procedures.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides a fixed bed bioreactor, the utility model discloses a design the jar body and the disc type carrier of a brand-new structure, optimized arranging of carrier to the growth of adherence cell on the carrier and the function of fixed bed bioreactor digestion cell have been optimized.
In order to solve the technical problem, the utility model discloses a following technical scheme:
in a first aspect, the present invention provides a fixed bed bioreactor, comprising:
the device comprises a tank body, a centrifugal stirrer, a top cover and a disc type carrier;
the tank body is cylindrical, and the top of the tank body is provided with an upward opening; the top cover covers the opening, the top cover is connected with the tank body in a sealing mode, and a mounting hole is formed in the top cover;
the centrifugal stirrer comprises a stirring shaft and stirring blades, the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body from the mounting hole, and one end of the stirring shaft and the stirring blades are positioned in the tank body;
the disc type carrier is arranged in the tank body, a through hole is formed in the circle center of the disc type carrier, and one end of the stirring shaft penetrates through the through hole of the disc type carrier.
Further, the stirring blade is positioned above the disc-shaped carrier.
Furthermore, the disc carriers are provided with a plurality of disc carriers, and the disc carriers are sequentially arranged along the length direction of the tank body.
Further, the fixed bed bioreactor also comprises a nutrient solution, the liquid level of the nutrient solution is higher than the stirring blades of the centrifugal stirrer, and at least part of the disc-type carrier is positioned in the nutrient solution.
Further, the centrifugal agitator further comprises: a power mechanism, a connecting piece and a sealing piece; at least part of the sealing element penetrates through the mounting hole and seals a gap between the inner side wall of the mounting hole and the stirring shaft;
the lower end of the connecting piece is connected with the other end of the stirring shaft, and the upper end of the connecting piece is connected with the power mechanism.
Furthermore, a plurality of flow guide holes are formed in the disc carrier and penetrate through the disc carrier in the axial direction.
Furthermore, a plurality of the diversion holes are arranged at intervals along the circumferential direction of the through hole of the disc type carrier; and/or
The diversion hole is in a strip shape and extends along the radial direction of the disc type carrier.
Further, the inner structure of the disc carrier comprises at least one of a honeycomb, tubular, porous or cylindrical structure; and/or
The material of the disc type carrier comprises at least one of glass, paper sheets and plastics.
Further, the fixed-bed bioreactor further comprises:
a bracket and a bolt;
the support comprises two circular rings arranged in parallel at intervals along the length direction of the tank body and a plurality of support rods for connecting the two circular rings;
the support sets up the outside of the jar body, the ring cover that is located upper portion is established the open-ended edge of the jar body, the top cap corresponds on with the ring that is located upper portion and is provided with a plurality of screws, the bolt passes the screw is connected the top cap with be located the ring on upper portion.
The utility model discloses an above-mentioned technical scheme's beneficial effect as follows:
the utility model provides a fixed bed bioreactor, fixed bed bioreactor includes: the device comprises a tank body, a centrifugal stirrer, a top cover and a disc type carrier; the tank body is cylindrical, and the top of the tank body is provided with an upward opening; the top cover covers the opening, the top cover is connected with the tank body in a sealing mode, and a mounting hole is formed in the top cover; the centrifugal stirrer comprises a stirring shaft and stirring blades, the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body from the mounting hole, and one end of the stirring shaft and the stirring blades are positioned in the tank body; the disc type carrier is arranged in the tank body, a through hole is formed in the circle center of the disc type carrier, and one end of the stirring shaft penetrates through the through hole of the disc type carrier. Be provided with a fixed hanging flower basket in being different from current fixed bed bioreactor to closely pile up the slice carrier in fixed hanging flower basket, the utility model provides a disc carrier is the inside of direct mount at the jar body, can the internal space of the make full use of jar body, just disc carrier can not pile up together, and the resistance is less when consequently nutrient solution flows, can guarantee that the velocity of flow of nutrient solution is fixed, the flow direction is unanimous and can not form the turbulent flow, thereby guarantees that nutrient substance distributes evenly, and then can guarantee that the cell growth state of load on disc carrier is unanimous. In addition, when the cells are digested, the pancreatin put in the tank body can also uniformly flow through the disc-shaped carrier along with the nutrient solution, so that the cells are conveniently separated from the disc-shaped carrier. Finally, the fixed bed bioreactor of the structure of the present invention is also convenient for cell expansion.
Drawings
FIG. 1 is a schematic view of a fixed bed bioreactor;
FIG. 2 is an exploded view of a fixed bed bioreactor;
FIG. 3 is a schematic representation of a disc support.
Reference numerals
A tank body 1, a centrifugal stirrer 2, a power mechanism 21, a connecting piece 22, a sealing piece 23,
Detailed Description
For further understanding of the present invention, preferred embodiments of the present invention will be described below with reference to examples, but it should be understood that these descriptions are only for the purpose of further illustrating the features and advantages of the present invention, and are not intended to limit the present invention.
The fixed bed bioreactor of the present invention will be further described with reference to the accompanying drawings.
In a first aspect, the present invention provides a fixed bed bioreactor, as shown in fig. 1 and 2, comprising: a tank body 1, a centrifugal stirrer 2, a top cover 3 and a disc type carrier 4. The tank body 1 is cylindrical, and the top of the tank body 1 is provided with an upward opening; the top cover 3 covers the opening, the top cover 3 is connected with the tank body 1 in a sealing mode, and a mounting hole is formed in the top cover 3; the centrifugal stirrer 2 comprises a stirring shaft and stirring blades, the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body 1 from the mounting hole, and one end of the stirring shaft and the stirring blades are positioned in the tank body 1; the disc support 4 may have a net-like structure or a pore-like structure to increase a contact area, promote the flow of a nutrient solution, and facilitate the uniform growth of cells. The disc type carrier 4 is arranged in the tank body 1, a through hole is formed in the circle center of the disc type carrier 4, and one end of the stirring shaft penetrates through the through hole of the disc type carrier 4.
Specifically, the utility model provides a cylindrical tank 1 and a disc type carrier 4 corresponding to the shape of the tank 1, which is different from the prior fixed bed bioreactor in that a fixed hanging basket is arranged and the disc type carrier is arranged in the fixed hanging basket. The utility model provides a disc carrier 4 is the inside of direct mount at jar body 1, can make full use of jar internal space of body 1, just disc carrier 4 can not be together piled up. The utility model discloses in centrifugal stirrer 2 starts just can drive the internal liquid flow of jar, liquid can select to be the nutrient solution, because the utility model provides a disc type carrier 4 direct mount just in the inside of jar body 1 disc type carrier 4 can not pile together, and the resistance is less when so nutrient solution flows, can guarantee that the velocity of flow of nutrient solution is fixed, the flow direction is unanimous and can not form the sinuous flow to guarantee that nutrient substance distributes evenly, can guarantee that the cell growth state of load on disc type carrier 4 is unanimous. In addition, when the cells are digested, the pancreatin put in the tank 1 can also flow through the disc 4 uniformly with the nutrient solution, thereby facilitating the separation of the cells from the disc 4. Finally, the fixed bed bioreactor of the structure of the present invention is also convenient for cell expansion.
According to some embodiments of the invention, the stirring blade is located above the disc support 4. The utility model discloses in stirring paddle leaf makes the nutrient solution circulation through centrifugal stirring, makes the cell on disc type carrier 4 can contact fresh nutrient substance.
According to the utility model discloses a some embodiments, disc carrier 4 has a plurality ofly, and is a plurality of disc carrier 4 follows the length direction of the jar body sets gradually, adjacent two disc carrier 4 can separate apart or contact, adjacent two the spacing distance between disc carrier 4 can be adjusted to adjust as required, in order to satisfy actual conditions's needs. The utility model provides a 1 inner wall of jar body is provided with a plurality of bayonets, disc carrier 4 passes through the bayonet socket is fixed in jar body 1, according to the length of jar body 1 and the spacing distance between disc carrier 4, 1 inside a jar body can set up a plurality of disc carrier 4, can cultivate adherent cell in a large number.
According to some embodiments of the present invention, the fixed-bed bioreactor further comprises a nutrient solution 5, wherein the liquid level of the nutrient solution 5 is higher than the stirring blades of the centrifugal stirrer 2, and at least part of the disc-type carrier 4 is located in the nutrient solution 5. The utility model provides a nutrient solution 5 circulates in the inside of jar body 1 under centrifugal stirrer 2's drive, flows through disc type carrier 4.
According to some embodiments of the present invention, the centrifugal stirrer 2 further comprises: a power mechanism 21, a connecting piece 22 and a sealing piece 23; at least part of the sealing element 23 passes through the mounting hole and seals a gap between the inner side wall of the mounting hole and the stirring shaft; the lower end of the connecting piece 22 is connected with the other end of the stirring shaft, and the upper end of the connecting piece 22 is connected with the power mechanism 21. The utility model provides a centrifugal stirrer 2 still includes power unit 21, connecting piece 22 and sealing member 23 except (mixing) shaft and stirring paddle leaf, power unit 21 does centrifugal stirrer 2 provides stirring power, sealing member 23 realizes top cap 3's leakproofness.
According to the utility model discloses a some embodiments, be equipped with a plurality of water conservancy diversion holes 41 on disc carrier 4, water conservancy diversion hole 41 is followed disc carrier 4's axial direction runs through, is favorable to nutrient solution 5 upwards flow from top to bottom for the cell growth is even, avoids the insufficient problem of local nutrient solution.
According to some embodiments of the present invention, a plurality of the guiding holes 41 are disposed at intervals along the circumferential direction of the through hole of the disc carrier 4; and/or the diversion hole 41 is strip-shaped, and the diversion hole 41 extends along the radial direction of the disc type carrier 4, so that the flowing of the nutrient solution 5 is facilitated, the cells grow uniformly, and the problem that the local nutrient solution 5 is insufficient is avoided. The diversion holes 41 on two adjacent disc carriers 4 can be arranged in a staggered way, so that the uniform flow of the nutrient solution 5 can be promoted.
Specifically, as shown in fig. 3, the disc-type carriers 4 are provided with a plurality of guiding holes 41, and the guiding holes 41 are provided to reduce the resistance of the nutrient solution 5 flowing between the disc-type carriers 4 and ensure the uniform circulation of the nutrient solution 5. The shape and number of the diversion holes 41 can be set according to actual requirements.
According to some embodiments of the present invention, the inner structure of the disc support 4 comprises at least one of a honeycomb, tubular, porous or cylindrical structure, increasing the surface area, facilitating the attachment and growth of cells; and/or the material of the disc carrier 4 comprises at least one of glass, paper and plastic. The utility model discloses the material of 4 inner structure of well disc carrier and disc carrier 4 can be selected according to the concrete adherent cell that need cultivate.
According to some embodiments of the invention, the fixed bed bioreactor further comprises:
The fixed bed bioreactor may further comprise: a material adds the pipe for detecting the temperature sensor of temperature, the oxygen content sensor that is used for detecting oxygen content, the pH valve detector that is used for detecting the pH valve and is used for adding the material, and temperature sensor, oxygen content sensor, pH valve detector can set up the inside of jar body 1, can set up other sensors according to actual conditions.
In a second aspect, the present invention provides the use of a fixed bed bioreactor as described above in cell culture. Utilize the utility model discloses in when the fixed bed bioreactor that provides carries out adherent cell culture, because the internal liquid flow direction of jar is unanimous, the velocity of flow is stable and can not take place the turbulent flow to can guarantee that nutrient substance distributes evenly, and then can guarantee that the cell growth state of load on disc type carrier 4 is unanimous. When the cells are digested, the pancreatin put in the tank body 1 can also flow through the disc-shaped carrier 4 uniformly along with the nutrient solution, thereby being convenient for separating the cells from the disc-shaped carrier 4. Finally, the fixed bed bioreactor of the structure of the present invention is also convenient for cell expansion.
Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by those of ordinary skill in the art to which the invention belongs. The use of "first," "second," and similar terms in the description herein do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships are changed accordingly.
The foregoing is a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A fixed bed bioreactor, comprising:
the device comprises a tank body, a centrifugal stirrer, a top cover and a disc type carrier;
the tank body is cylindrical, and the top of the tank body is provided with an upward opening; the top cover covers the opening, is connected with the tank body in a sealing manner and is provided with a mounting hole;
the centrifugal stirrer comprises a stirring shaft and stirring blades, the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body from the mounting hole, and one end of the stirring shaft and the stirring blades are positioned in the tank body;
the disc type carrier is arranged in the tank body, a through hole is formed in the circle center of the disc type carrier, and one end of the stirring shaft penetrates through the through hole of the disc type carrier.
2. A fixed bed bioreactor as claimed in claim 1 wherein the stirring blades are located above the disc support.
3. A fixed bed bioreactor as claimed in claim 1 wherein the disc carriers are plural and are arranged in series along the length of the tank.
4. A fixed bed bioreactor as claimed in claim 1 further comprising a nutrient solution in the fixed bed bioreactor, the level of the nutrient solution being above the stirring blades of the centrifugal stirrer, at least part of the disc-type carrier being located in the nutrient solution.
5. A fixed bed bioreactor as set forth in claim 1 wherein said centrifugal agitator further comprises:
a power mechanism, a connecting piece and a sealing piece; at least part of the sealing element penetrates through the mounting hole and seals a gap between the inner side wall of the mounting hole and the stirring shaft;
the lower end of the connecting piece is connected with the other end of the stirring shaft, and the upper end of the connecting piece is connected with the power mechanism.
6. A fixed bed bioreactor as claimed in claim 1 wherein the disc support is provided with a plurality of flow directing holes extending through the disc support in the axial direction of the disc support.
7. A fixed bed bioreactor as claimed in claim 6 wherein a plurality of said deflector holes are provided at intervals along the circumference of said through holes of said disc carrier; and/or
The diversion hole is in a strip shape and extends along the radial direction of the disc type carrier.
8. A fixed bed bioreactor as claimed in claim 1 wherein the internal structure of the disc support comprises at least one of a honeycomb, tubular, perforated or cylindrical structure; and/or
The material of the disc type carrier comprises at least one of glass, paper sheets and plastics.
9. A fixed bed bioreactor as set forth in claim 1 further comprising:
a bracket and a bolt;
the support comprises two circular rings arranged in parallel at intervals along the length direction of the tank body and a plurality of support rods for connecting the two circular rings;
the support sets up the outside of the jar body, the ring cover that is located upper portion is established the open-ended edge of the jar body, the top cap corresponds on with the ring that is located upper portion and is provided with a plurality of screws, the bolt passes the screw is connected the top cap with be located the ring on upper portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121441817.4U CN216838000U (en) | 2021-06-28 | 2021-06-28 | Fixed bed bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121441817.4U CN216838000U (en) | 2021-06-28 | 2021-06-28 | Fixed bed bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216838000U true CN216838000U (en) | 2022-06-28 |
Family
ID=82082517
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121441817.4U Active CN216838000U (en) | 2021-06-28 | 2021-06-28 | Fixed bed bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216838000U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115369036A (en) * | 2022-10-21 | 2022-11-22 | 天信和(苏州)生物科技有限公司 | Cell culture carrier assembly and bioreactor |
-
2021
- 2021-06-28 CN CN202121441817.4U patent/CN216838000U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115369036A (en) * | 2022-10-21 | 2022-11-22 | 天信和(苏州)生物科技有限公司 | Cell culture carrier assembly and bioreactor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2001113C (en) | Bioreactor | |
| EP0213691B1 (en) | A bioconversion reactor | |
| CN216838000U (en) | Fixed bed bioreactor | |
| WO1991000339A1 (en) | Rotary culture device | |
| US20140242689A1 (en) | Vortex bioreactor | |
| CN112708562A (en) | Fixed bed type bioreactor | |
| JPH0646832A (en) | Bioreactor | |
| US5316945A (en) | Cell carrier arrangement | |
| JPH06510205A (en) | Biomass particle growth method and device | |
| CN114736797A (en) | Cell culture reactor | |
| CN215162831U (en) | Carrier and bioreactor for culturing cells | |
| CN102092848B (en) | Anaerobic reactor of horizontal flow fixed bed | |
| CN104195044A (en) | Centrifuging lifting stirrer of bioreactor | |
| US6632657B1 (en) | Apparatus for cultivating tissue cells and microorganisms in suspension | |
| CN112358039B (en) | Moving bed biofilm reactor and sewage treatment system | |
| CN115595263A (en) | Fixed bed bioreactor | |
| CN215162823U (en) | Carrier and bioreactor for culturing cells | |
| RU2471863C2 (en) | Bioreactor and method of culturing photosynthesising microorganisms using said bioreactor | |
| CN217677577U (en) | Detachable fixed bed bioreactor suitable for viral vector production | |
| CN212955179U (en) | From fixed bed and bioreactor of taking fluid dispersion function | |
| US11198840B2 (en) | Assembled bioreactor chamber suitable for perfusion culture | |
| CN211972297U (en) | Cell separation device for bioreactor | |
| CN112375685A (en) | Fixed bed bioreactor | |
| CN210215327U (en) | Device for preparing ethanol by fermentation of immobilized yeast | |
| CN217202690U (en) | Microbial fermentation and stirring reactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |