CN115595263A - Fixed bed bioreactor - Google Patents

Fixed bed bioreactor Download PDF

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Publication number
CN115595263A
CN115595263A CN202110718257.0A CN202110718257A CN115595263A CN 115595263 A CN115595263 A CN 115595263A CN 202110718257 A CN202110718257 A CN 202110718257A CN 115595263 A CN115595263 A CN 115595263A
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CN
China
Prior art keywords
disc
tank body
fixed bed
bed bioreactor
carrier
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202110718257.0A
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Chinese (zh)
Inventor
陈文庆
周劲松
陈晨晨
史洋
赵洪磊
王杰勇
刘勇峰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianxinhe Suzhou Biotechnology Co ltd
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Tianxinhe Suzhou Biotechnology Co ltd
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Publication date
Application filed by Tianxinhe Suzhou Biotechnology Co ltd filed Critical Tianxinhe Suzhou Biotechnology Co ltd
Priority to CN202110718257.0A priority Critical patent/CN115595263A/en
Publication of CN115595263A publication Critical patent/CN115595263A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C12M25/18—Fixed or packed bed

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention provides a fixed bed bioreactor comprising: the device comprises a tank body, a centrifugal stirrer, a top cover and a disc type carrier; the disc type carrier is arranged in the tank body, a through hole is formed in the circle center of the disc type carrier, and one end of a stirring shaft of the centrifugal stirrer penetrates through the through hole of the disc type carrier. The disc-type carriers are directly arranged in the tank body, the internal space of the tank body can be fully utilized, and the disc-type carriers are not stacked together, so that the resistance of the flowing nutrient solution is small, the flow velocity of the nutrient solution is fixed, the flow direction of the nutrient solution is consistent, no turbulent flow is formed, the nutrient substances are uniformly distributed, and the growth state of cells loaded on the disc-type carriers is consistent. In addition, when the cells are digested, the pancreatin can also uniformly flow through the disc carrier along with the nutrient solution, thereby facilitating the separation of the cells from the disc carrier.

Description

Fixed bed bioreactor
Technical Field
The invention relates to the technical field of bioreactors, in particular to a fixed bed bioreactor.
Background
According to the cell characteristics, adherent cells and suspension cells are generally divided, wherein the adherent cells need to be attached to a carrier for growth, and the suspension cells can independently grow. The carriers for adherent cell culture are generally classified into spherical carriers and sheet carriers. In order to meet the requirements of carrier surface area, cell density, cell activity and the like, the spherical carrier is generally a 100-120 mu m small sphere, and the sheet-shaped carrier is generally a strip-shaped or round paper sheet.
In the existing fixed bed bioreactor, a fixed hanging basket is formed by two mesh baffles and a tank body, a flaky carrier is arranged in the fixed hanging basket, adherent cells and a culture medium flow through the fixed hanging basket through a bioreactor stirring system, the adherent cells are adsorbed on the flaky carrier in the fixed hanging basket due to the adherent capacity of the adherent cells, and the culture medium flows through the flaky carrier through the bioreactor stirring system and provides nutrition for the cells, so that the growth and proliferation of the cells are maintained. However, in the prior art, the sheet-shaped carriers are limited in arrangement in the fixed hanging basket, and can only be stacked together in multiple layers, and the sheet-shaped carriers are tightly extruded, so that the resistance of cells to be cultured and a culture medium is large when the cells to be cultured and the culture medium flow through the sheet-shaped carriers, the flow path is short, and the cells and the culture medium are difficult to be uniformly distributed inside and outside. Meanwhile, because adherent cells are adsorbed on the closely stacked sheet-shaped carriers, the cells are difficult to separate from the carriers during digestion. In addition, the conventional fixed-bed bioreactor has the problems that the carrier loading is inconvenient and the cells are difficult to expand again. The common defects of the spherical carrier and the flaky carrier are higher cost, poor repeated use effect and complex digestion and amplification operation procedures.
Disclosure of Invention
In view of the above, the invention provides a fixed bed bioreactor, and the invention optimizes the arrangement of carriers by designing a tank body and a disc type carrier with a brand new structure, thereby optimizing the growth of adherent cells on the carriers and the function of digesting the cells by the fixed bed bioreactor.
In order to solve the technical problems, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a fixed bed bioreactor comprising:
the device comprises a tank body, a centrifugal stirrer, a top cover and a disc type carrier;
the tank body is cylindrical, and the top of the tank body is provided with an upward opening; the top cover covers the opening, is connected with the tank body in a sealing manner and is provided with a mounting hole;
the centrifugal stirrer comprises a stirring shaft and stirring blades, the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body from the mounting hole, and one end of the stirring shaft and the stirring blades are positioned in the tank body;
the disc type carrier is arranged in the tank body, a through hole is formed in the circle center of the disc type carrier, and one end of the stirring shaft penetrates through the through hole of the disc type carrier.
Further, the stirring blade is positioned above the disc-shaped carrier.
Furthermore, the disc carriers are provided with a plurality of disc carriers, and the disc carriers are sequentially arranged along the length direction of the tank body.
Further, the fixed bed bioreactor also comprises a nutrient solution, the liquid level of the nutrient solution is higher than the stirring blades of the centrifugal stirrer, and at least part of the disc-type carrier is positioned in the nutrient solution.
Further, the centrifugal agitator further comprises: a power mechanism, a connecting piece and a sealing piece; at least part of the sealing element penetrates through the mounting hole and seals a gap between the inner side wall of the mounting hole and the stirring shaft;
the lower end of the connecting piece is connected with the other end of the stirring shaft, and the upper end of the connecting piece is connected with the power mechanism.
Furthermore, a plurality of flow guide holes are formed in the disc carrier and penetrate through the disc carrier in the axial direction.
Furthermore, a plurality of the diversion holes are arranged at intervals along the circumferential direction of the through hole of the disc type carrier; and/or
The diversion hole is in a strip shape and extends along the radial direction of the disc type carrier.
Further, the inner structure of the disc carrier comprises at least one of a honeycomb, tubular, porous or cylindrical structure; and/or
The material of the disc-type carrier comprises at least one of glass, paper sheets and plastics.
Further, the fixed-bed bioreactor further comprises:
a bracket and a bolt;
the support comprises two circular rings arranged in parallel at intervals along the length direction of the tank body and a plurality of support rods for connecting the two circular rings;
the support sets up the outside of the jar body, the ring cover that is located upper portion is established the open-ended edge of the jar body, the top cap corresponds on with the ring that is located upper portion and is provided with a plurality of screws, the bolt passes the screw is connected the top cap with be located the ring on upper portion.
The technical scheme of the invention has the following beneficial effects:
the present invention provides a fixed bed bioreactor comprising: the device comprises a tank body, a centrifugal stirrer, a top cover and a disc type carrier; the tank body is cylindrical, and the top of the tank body is provided with an upward opening; the top cover covers the opening, is connected with the tank body in a sealing manner and is provided with a mounting hole; the centrifugal stirrer comprises a stirring shaft and stirring blades, the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body from the mounting hole, and one end of the stirring shaft and the stirring blades are positioned in the tank body; the disc type carrier is arranged in the tank body, a through hole is formed in the circle center of the disc type carrier, and one end of the stirring shaft penetrates through the through hole of the disc type carrier. The invention is characterized in that a fixed hanging basket is arranged in the existing fixed bed bioreactor, and the sheet carriers are tightly stacked in the fixed hanging basket, the disc carriers in the invention are directly arranged in the tank body, the internal space of the tank body can be fully utilized, and the disc carriers are not stacked together, so that the resistance is small when the nutrient solution flows, the flow velocity of the nutrient solution is fixed, the flow direction is consistent, and the turbulent flow is not formed, thereby ensuring that the nutrient substance is uniformly distributed, and ensuring that the growth states of cells loaded on the disc carriers are consistent. In addition, when the cells are digested, the pancreatin put in the tank body can also uniformly flow through the disc-shaped carrier along with the nutrient solution, so that the cells are conveniently separated from the disc-shaped carrier. Finally, the fixed-bed bioreactor of the present invention also facilitates cell expansion.
Drawings
FIG. 1 is a schematic view of the structure of a fixed-bed bioreactor;
FIG. 2 is an exploded view of a fixed bed bioreactor;
FIG. 3 is a schematic diagram of a disc carrier.
Reference numerals
A tank body 1, a centrifugal stirrer 2, a power mechanism 21, a connecting piece 22, a sealing piece 23,
Top cap 3, disc carrier 4, water conservancy diversion hole 41, nutrient solution 5, support 6, bolt 7.
Detailed Description
For a further understanding of the invention, reference will now be made to the preferred embodiments of the invention in conjunction with the following examples, but it will be understood that the description is intended to illustrate the features and advantages of the invention further, and not to limit the invention.
The fixed-bed bioreactor of the present invention is further described below with reference to the accompanying drawings.
In a first aspect, the present invention provides a fixed bed bioreactor, as shown in fig. 1 and 2, comprising: a tank body 1, a centrifugal stirrer 2, a top cover 3 and a disc type carrier 4. The tank body 1 is cylindrical, and the top of the tank body 1 is provided with an upward opening; the top cover 3 covers the opening, the top cover 3 is connected with the tank body 1 in a sealing mode, and a mounting hole is formed in the top cover 3; the centrifugal stirrer 2 comprises a stirring shaft and stirring blades, the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body 1 from the mounting hole, and one end of the stirring shaft and the stirring blades are positioned in the tank body 1; the disc-type carriers 4 may have a net-shaped structure or a pore-shaped structure to increase a contact area, promote the flow of a nutrient solution, and facilitate the uniform growth of cells. The disc type carrier 4 is arranged in the tank body 1, a through hole is formed in the circle center of the disc type carrier 4, and one end of the stirring shaft penetrates through the through hole of the disc type carrier 4.
Specifically, unlike the conventional fixed bed bioreactor in which a fixed basket is provided and a disc-shaped carrier is placed in the fixed basket, the present invention provides a cylindrical tank 1 and a disc-shaped carrier 4 corresponding to the shape of the tank 1. The disc carriers 4 of the present invention are directly installed inside the can body 1, the inner space of the can body 1 can be fully utilized, and the disc carriers 4 are not stacked together. The centrifugal stirrer 2 can drive liquid in the tank body to flow when being started, the liquid can be selected as nutrient solution, and the disc type carriers 4 in the invention are directly arranged in the tank body 1 and the disc type carriers 4 are not stacked together, so that the resistance of the flowing nutrient solution is small, the fixed flow speed and the consistent flow direction of the nutrient solution can be ensured, the turbulent flow can not be formed, the uniform distribution of nutrient substances can be ensured, and the consistent growth state of cells loaded on the disc type carriers 4 can be ensured. In addition, when digesting cells, pancreatin put in the tank body 1 can also flow through the disc carrier 4 uniformly with the nutrient solution, thereby facilitating the separation of the cells from the disc carrier 4. Finally, the fixed-bed bioreactor of the present invention also facilitates cell expansion.
According to some embodiments of the invention, the stirring blade is located above the disc support 4. The stirring paddle of the present invention circulates the nutrient solution by centrifugal stirring so that the cells on the disc support 4 can contact fresh nutrients.
According to some embodiments of the present invention, the number of the disc carriers 4 is multiple, multiple disc carriers 4 are sequentially arranged along the length direction of the tank, two adjacent disc carriers 4 can be spaced apart or contacted, and the spacing distance between two adjacent disc carriers 4 can be adjusted, so as to be adjusted as required to meet the needs of actual conditions. According to the invention, the inner wall of the tank body 1 is provided with a plurality of bayonets, the disc-type carriers 4 are fixed in the tank body 1 through the bayonets, and a plurality of disc-type carriers 4 can be arranged in the tank body 1 according to the length of the tank body 1 and the spacing distance between the disc-type carriers 4, so that adherent cells can be cultured in a large quantity.
According to some embodiments of the invention, the fixed-bed bioreactor further comprises a nutrient solution 5, the liquid level of the nutrient solution 5 is higher than the stirring blades of the centrifugal stirrer 2, and at least part of the disc-shaped carrier 4 is positioned in the nutrient solution 5. The nutrient solution 5 of the invention circulates in the tank body 1 under the drive of the centrifugal stirrer 2 and flows through the disc-type carrier 4.
According to some embodiments of the invention, the centrifugal stirrer 2 further comprises: a power mechanism 21, a connecting piece 22 and a sealing piece 23; at least part of the sealing element 23 passes through the mounting hole and seals a gap between the inner side wall of the mounting hole and the stirring shaft; the lower end of the connecting piece 22 is connected with the other end of the stirring shaft, and the upper end of the connecting piece 22 is connected with the power mechanism 21. The centrifugal stirrer 2 comprises a power mechanism 21, a connecting piece 22 and a sealing piece 23 besides a stirring shaft and a stirring blade, wherein the power mechanism 21 provides stirring power for the centrifugal stirrer 2, and the sealing piece 23 realizes the sealing performance of the top cover 3.
According to some embodiments of the present invention, a plurality of diversion holes 41 are formed in the disc carrier 4, and the diversion holes 41 penetrate through the disc carrier 4 in the axial direction, so as to facilitate the flow of the nutrient solution 5 in the up-down direction, so that the cells grow uniformly, and the problem of insufficient local nutrient solution is avoided.
According to some embodiments of the present invention, a plurality of the guiding holes 41 are arranged at intervals along the circumference of the through hole of the disc carrier 4; and/or the diversion hole 41 is strip-shaped, and the diversion hole 41 extends along the radial direction of the disc type carrier 4, so that the flowing of the nutrient solution 5 is facilitated, the cells grow uniformly, and the problem that the local nutrient solution 5 is insufficient is avoided. The diversion holes 41 on two adjacent disc carriers 4 can be arranged in a staggered way, so that the uniform flow of the nutrient solution 5 can be promoted.
Specifically, as shown in fig. 3, the disc-type carriers 4 are provided with a plurality of guiding holes 41, and the guiding holes 41 are provided to reduce the resistance of the nutrient solution 5 flowing between the disc-type carriers 4 and ensure the uniform circulation of the nutrient solution 5. The shape and number of the diversion holes 41 can be set according to actual requirements.
According to some embodiments of the invention, the internal structure of the disc support 4 comprises at least one of a honeycomb, tubular, porous or cylindrical structure, increasing the surface area, facilitating the attachment and growth of cells; and/or the material of the disc carrier 4 comprises at least one of glass, paper and plastic. The internal structure of the disc carrier 4 and the material of the disc carrier 4 can be selected according to the specific adherent cells to be cultured.
According to some embodiments of the invention, the fixed-bed bioreactor further comprises:
bracket 6 and bolt 7; the support 6 comprises two circular rings arranged in parallel at intervals along the length direction of the tank body 1 and a plurality of support rods for connecting the two circular rings; support 6 sets up the outside of the jar body 1, the ring cover that is located upper portion is established the open-ended edge of the jar body 1, top cap 3 corresponds on the ring with be located upper portion and is provided with a plurality of screws, bolt 7 passes the screw is connected top cap 3 and the ring that is located upper portion. According to the invention, on one hand, the support 6 realizes the function of supporting the tank body 1, on the other hand, a plurality of screw holes are formed in the circular ring at the upper part of the support 6, the screw holes are correspondingly formed in the top cover 3, and the bolts 7 penetrate through the screw holes to connect the top cover 3 and the circular ring at the upper part of the support 6, so that the top cover 3 and the support 6 are connected, and the function of sealing the tank body 1 is achieved.
The fixed bed bioreactor may further comprise: a material adds the pipe for detecting the temperature sensor of temperature, the oxygen content sensor that is used for detecting oxygen content, the pH valve detector that is used for detecting the pH valve and is used for adding the material, and temperature sensor, oxygen content sensor, pH valve detector can set up the inside of jar body 1, can set up other sensors according to actual conditions.
In a second aspect, the present invention provides the use of a fixed bed bioreactor as described above in cell culture. When the fixed bed bioreactor provided by the invention is used for culturing adherent cells, the liquid in the tank body flows in the same direction, the flow rate is stable, and the turbulent flow cannot occur, so that the nutrient substances can be uniformly distributed, and the growth state of the cells loaded on the disc type carrier 4 can be ensured to be consistent. When the cells are digested, the pancreatin put in the tank body 1 can also flow through the disc-shaped carrier 4 uniformly along with the nutrient solution, thereby being convenient for separating the cells from the disc-shaped carrier 4. Finally, the fixed-bed bioreactor of the present invention also facilitates cell expansion.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The use of "first," "second," and the like, herein does not denote any order, quantity, or importance, but rather the terms "first," "second," and the like are used to distinguish one element from another. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships are changed accordingly.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (9)

1. A fixed bed bioreactor, comprising:
the device comprises a tank body, a centrifugal stirrer, a top cover and a disc-type carrier;
the tank body is cylindrical, and the top of the tank body is provided with an upward opening; the top cover covers the opening, the top cover is connected with the tank body in a sealing mode, and a mounting hole is formed in the top cover;
the centrifugal stirrer comprises a stirring shaft and stirring blades, the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body from the mounting hole, and one end of the stirring shaft and the stirring blades are positioned in the tank body;
the disc type carrier is arranged in the tank body, a through hole is formed in the circle center of the disc type carrier, and one end of the stirring shaft penetrates through the through hole of the disc type carrier.
2. A fixed bed bioreactor as claimed in claim 1 wherein the stirring blades are located above the disc support.
3. A fixed bed bioreactor as claimed in claim 1 wherein the disc carriers are plural and are arranged in series along the length of the tank.
4. A fixed bed bioreactor as claimed in claim 1 further comprising a nutrient solution in the fixed bed bioreactor, the level of the nutrient solution being above the stirring blades of the centrifugal stirrer, at least part of the disc-type carrier being located in the nutrient solution.
5. A fixed bed bioreactor as set forth in claim 1 wherein said centrifugal agitator further comprises:
a power mechanism, a connecting piece and a sealing piece; at least part of the sealing element penetrates through the mounting hole and seals a gap between the inner side wall of the mounting hole and the stirring shaft;
the lower end of the connecting piece is connected with the other end of the stirring shaft, and the upper end of the connecting piece is connected with the power mechanism.
6. A fixed bed bioreactor as claimed in claim 1 wherein the disc support is provided with a plurality of flow directing holes extending through the disc support in the axial direction of the disc support.
7. A fixed bed bioreactor as claimed in claim 6 wherein a plurality of said deflector holes are provided at intervals along the circumference of said through holes of said disc carrier; and/or
The diversion hole is in a strip shape and extends along the radial direction of the disc type carrier.
8. A fixed bed bioreactor as claimed in claim 1 wherein the internal structure of the disc support comprises at least one of a honeycomb, tubular, perforated or cylindrical structure; and/or
The material of the disc type carrier comprises at least one of glass, paper sheets and plastics.
9. A fixed bed bioreactor as set forth in claim 1, further comprising:
a bracket and a bolt;
the support comprises two circular rings arranged in parallel at intervals along the length direction of the tank body and a plurality of support rods for connecting the two circular rings;
the support sets up the outside of the jar body, the ring cover that is located upper portion is established the open-ended edge of the jar body, the top cap corresponds on with the ring that is located upper portion and is provided with a plurality of screws, the bolt passes the screw is connected the top cap with be located the ring on upper portion.
CN202110718257.0A 2021-06-28 2021-06-28 Fixed bed bioreactor Pending CN115595263A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110718257.0A CN115595263A (en) 2021-06-28 2021-06-28 Fixed bed bioreactor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110718257.0A CN115595263A (en) 2021-06-28 2021-06-28 Fixed bed bioreactor

Publications (1)

Publication Number Publication Date
CN115595263A true CN115595263A (en) 2023-01-13

Family

ID=84840359

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110718257.0A Pending CN115595263A (en) 2021-06-28 2021-06-28 Fixed bed bioreactor

Country Status (1)

Country Link
CN (1) CN115595263A (en)

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