CN215162832U - Carrier and bioreactor for culturing cells - Google Patents

Abstract

The utility model provides a carrier and bioreactor for cultivating cells, the carrier includes: the bearing bodies are provided with connecting areas and bearing areas, the connecting areas on two adjacent bearing bodies are connected, and at least parts of the bearing areas on two adjacent bearing bodies are spaced. In the application process, the carrier can be placed in the culture solution, the cells can be attached to the bearing areas on the bearing bodies, the cells continuously grow in the bearing areas, at least parts of the bearing areas on two adjacent bearing bodies are spaced, the flowing of the culture solution is facilitated, the uniform growth of the cells is facilitated, the digestion and amplification operation procedures are simple, and the cells are conveniently digested and amplified step by step.

Description

Carrier and bioreactor for culturing cells

Technical Field

The utility model relates to the technical field of biology, concretely relates to a carrier and bioreactor for cultivating cells.

Background

In the process of cell culture, adherent cells need to be attached to a carrier for growth, and the carrier for adherent cell culture needs to meet the requirements of carrier surface area, cell density, cell activity and the like. The existing carrier cell culture has poor effect, complicated digestion and amplification operation procedures and no capability of digesting cells and amplifying the cells step by step.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model provides a carrier and bioreactor for culturing cell for solve the current carrier cell culture's the not good effect, the difficult cell of digestion and enlarge the problem step by step.

In order to solve the technical problem, the utility model discloses a following technical scheme:

in a first aspect, the present invention provides a carrier for culturing cells, comprising:

the bearing bodies are provided with connecting areas and bearing areas, the connecting areas on two adjacent bearing bodies are connected, and at least parts of the bearing areas on two adjacent bearing bodies are spaced.

Wherein, the supporting body is in a sheet shape, and the connecting area is positioned at the edge or the central position of the supporting body.

Wherein, the supporting body is a hollow cone, and the connecting area is positioned at the top of the cone.

Wherein the axes of each of the cones are collinear.

Wherein, still include:

and the supporting part is arranged between the bearing areas on two adjacent bearing bodies.

Wherein, still include:

the first plate body and the second plate body are arranged in parallel at intervals, and the bearing body is located between the first plate body and the second plate body.

The first plate body is provided with a first flow guide hole penetrating along the thickness direction of the first plate body, and the second plate body is provided with a second flow guide hole penetrating along the thickness direction of the second plate body.

Wherein the carrier comprises at least one of a honeycomb structure, a pore-like structure, and a lattice structure; and/or

The supporting body is provided with a through hole penetrating along the thickness direction of the supporting body; and/or

The supporting body is circular, oval or polygonal; and/or

The surface of the bearing area on the bearing body is provided with a microstructure, and the microstructure comprises at least one of a strip-shaped protrusion, a strip-shaped groove and a granular protrusion; and/or

A net structure is arranged between every two adjacent bearing bodies.

In a second aspect, the present invention provides a bioreactor comprising:

the carrier described in the above embodiments;

the jar body, the jar has feed inlet and discharge gate on the body, the carrier set up in the jar body.

Wherein, still include:

the stirrer comprises a stirring shaft and stirring blades, wherein the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body, and the stirring blades are positioned in the tank body.

The utility model discloses an above-mentioned technical scheme's beneficial effect as follows:

according to the utility model discloses a carrier for cultivating cell includes: the bearing bodies are provided with connecting areas and bearing areas, the connecting areas on two adjacent bearing bodies are connected, and at least parts of the bearing areas on two adjacent bearing bodies are spaced. In the application process, the carrier can be placed in the culture solution, the cells can be attached to the bearing areas on the bearing bodies, the cells continuously grow in the bearing areas, at least parts of the bearing areas on two adjacent bearing bodies are spaced, the flowing of the culture solution is facilitated, the uniform growth of the cells is facilitated, the digestion and amplification operation procedures are simple, and the cells are conveniently digested and amplified step by step.

Drawings

Fig. 1 is an exploded view of a carrier according to an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a carrier according to an embodiment of the present invention;

FIG. 3 is a schematic structural view of the carrier;

figure 4 is another schematic view of the carrier;

fig. 5 is a schematic structural diagram of the first plate body;

fig. 6 is a schematic structural view of the second plate;

FIG. 7 is a schematic view of the structure of the bioreactor.

Reference numerals

A carrier 10; a joining zone 11; a carrier region 12; a support portion 13;

a first plate body 21; a second plate 22;

first flow guide holes 23; second flow guide holes 24;

a tank 30; a stirring shaft 31; the stirring blade 32.

Detailed Description

For further understanding of the present invention, preferred embodiments of the present invention will be described below with reference to examples, but it should be understood that these descriptions are only for the purpose of further illustrating the features and advantages of the present invention, and are not intended to limit the present invention.

As shown in fig. 1 to 6, the present invention provides a carrier for culturing cells, comprising: at least two supporting bodies 10, the supporting bodies 10 are provided with a connecting area and a bearing area 12, the connecting areas 11 on two adjacent supporting bodies 10 are connected, the connecting areas 11 on two adjacent supporting bodies 10 can be directly connected or connected through a connecting piece, the connecting areas can be provided with connecting holes, a plurality of supporting bodies 10 can be arranged in a penetrating way or connected together through the connecting holes, at least parts of the bearing areas 12 on two adjacent supporting bodies 10 are spaced, for example, the bearing areas 12 on two adjacent supporting bodies 10 are spaced, and the uniform flow of the culture solution is facilitated. The supporting body 10 can be an elastic member, such as a plastic member or a rubber member, so that the supporting body 10 can be deformed to prevent the supporting body from being damaged when being extruded from the outside. The carrier 10 may be a transparent member to facilitate viewing of the condition on the carrier 10. In the application process, the carrier can be placed in the culture solution, the cells can be attached to the bearing areas 12 on the bearing bodies 10, the cells continuously grow in the bearing areas 12, at least parts of the bearing areas 12 on two adjacent bearing bodies 10 are spaced, the flowing of the culture solution is facilitated, the uniform growth of the cells is facilitated, the digestion and amplification operation procedure is simple, and the cells are conveniently digested and amplified step by step.

In some embodiments, the carrier 10 may be substantially sheet-like, and the connection zone 11 may be located at the edge or center of the carrier 10, for example, the connection zone 11 may be located at the edge of the carrier 10 to facilitate connection. The connecting region 11 may be located at the center of the supporting body 10, and other regions of the supporting body 10 may be used as supporting regions to facilitate cell attachment.

In other embodiments, the supporting body 10 may be a hollow cone, the connecting region 11 is located at the top of the cone to facilitate the connection between two adjacent supporting bodies 10, and the other region of the cone is used as a supporting region to facilitate the attachment of cells, facilitate the digestion and amplification of cells, and facilitate the digestion and amplification of cells step by step.

Optionally, the axes of each cone are collinear, and two adjacent cones can be sleeved together, so that the surface area is increased, and uniform growth of cells is facilitated.

According to some embodiments, further comprising: the supporting parts 13 are arranged between the bearing areas 12 on two adjacent bearing bodies 10, the supporting parts 13 can be provided with a plurality of supporting parts 13, the supporting parts 13 can be arranged at intervals, and the supporting parts 13 can have a supporting function, so that the bearing areas 12 on two adjacent bearing bodies 10 can be spaced apart and are not easy to collapse, and the flowing of the culture solution is facilitated. The supporting portion 13 can be an elastic member, such as a rubber member, because the supporting portion 13 has elasticity, and has a buffering effect, when the bearing areas 12 on two adjacent bearing bodies 10 are squeezed by an external acting force, the supporting portion 13 can be deformed in a buffering manner, and after the external acting force is eliminated, the bearing areas 12 on the bearing bodies 10 can be restored to the original shape, so that the damage or the edge of the bearing areas 12 is avoided. The supporting portion 13 may be a net structure, and the net structure may be disposed on one or two sides of the carrying region 12, so that the carrying region 12 on the carrying body 10 may be supported by the net structure, thereby enhancing the overall strength.

Optionally, the method further comprises: the first board body 21 and the second board body 22 are arranged in parallel and spaced apart, the carrier 10 is located between the first board body 21 and the second board body 22, and the carrier 10 can be fixed by the first board body 21 and the second board body 22. Wherein, be equipped with the first water conservancy diversion hole 23 that runs through along the thickness direction of first plate body 21 on the first plate body 21, be equipped with the second water conservancy diversion hole 24 that runs through along the thickness direction of second plate body 22 on the second plate body 22, the quantity of first water conservancy diversion hole 23 and second water conservancy diversion hole 24 can have a plurality ofly respectively, can evenly distributed, is favorable to the flow of culture solution through first water conservancy diversion hole 23 and second water conservancy diversion hole 24.

Alternatively, the carrier 10 may include at least one of a honeycomb structure, a pore-like structure, and a lattice structure, which may increase a surface area to facilitate the flow of the culture solution.

In some embodiments, the carrier 10 may be provided with through holes 14 penetrating along the thickness direction of the carrier 10, the through holes 14 may be long, the number of the through holes 14 may be multiple, and the through holes may be uniformly distributed, which is beneficial to uniform flow of the culture solution.

In other embodiments, the carrier 10 may be circular, oval or polygonal, and the specific shape may be selected according to actual conditions.

Alternatively, the surface of the bearing region on the bearing body 10 may have a microstructure, the microstructure may be uniformly spaced and distributed, and the microstructure may include at least one of an elongated protrusion, an elongated groove and a granular protrusion, for example, the microstructure may be an elongated protrusion, and the plurality of elongated protrusions are uniformly spaced and distributed, so that the surface area may be increased through the microstructure, the space for cell growth may be increased, and cell growth may be facilitated.

Optionally, a net structure may be disposed between two adjacent supporting bodies 10, the net structure may serve as a supporting framework and has a supporting effect on the supporting bodies 10, the net structure may be a plastic net, a fiber net or a metal net, the surface area may be increased by the net structure, the supporting effect may be provided for the supporting bodies 10, and the overall strength of the supporting bodies 10 is enhanced.

Optionally, at least one side surface of the supporting body 10 is provided with a net structure, for example, at least one side surface of the supporting region on the supporting body 10 may be provided with a net structure, the net structure may be a plastic net, a fiber net or a metal net, the net structure may increase the surface area, and may have a supporting effect on the supporting body 10, so as to enhance the overall strength of the supporting body 10. For example, the supporting body 10 is a plastic layer, and one side of the supporting body 10 is provided with a net structure, through which the supporting body 10 can be supported. A layer of net structure may be disposed between two adjacent carriers 10, for example, a net structure may be disposed on the surface of the carrying region on the carrier 10.

As shown in fig. 7, the present invention provides a bioreactor comprising:

the carrier and the tank body 30 in the above embodiment have a feed inlet and a discharge outlet on the tank body 30, and can feed materials through the feed inlet and discharge materials through the discharge outlet, the carrier is arranged in the tank body 30, and the number of the carriers can be multiple. In the application process, the culture solution can be added into the tank body 30, the carrier is placed in the culture solution, the cells can be attached to the bearing areas 12 on the bearing bodies 10, the cells continuously grow in the bearing areas 12, at least parts of the bearing areas 12 on two adjacent bearing bodies 10 are spaced, the flow of the culture solution is facilitated, the uniform growth of the cells is facilitated, the digestion and amplification operation procedures are simple, and the cells are conveniently digested and amplified step by step.

In some embodiments, the bioreactor further comprises: the agitator, the agitator include (mixing) shaft 31 and stirring paddle 32, and stirring paddle 32 establishes on (mixing) shaft 31, and the one end of (mixing) shaft 31 stretches into jar body 30, and stirring paddle 32 is arranged in jar body 30, can stir the culture solution through stirring paddle 32, is favorable to promoting the even flow of culture solution.

Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by those of ordinary skill in the art to which the invention belongs. The use of "first," "second," and similar terms in the description herein do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships are changed accordingly.

The foregoing is a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. A carrier for culturing cells, comprising:

the bearing bodies are provided with connecting areas and bearing areas, the connecting areas on two adjacent bearing bodies are connected, and at least parts of the bearing areas on two adjacent bearing bodies are spaced.

2. The carrier of claim 1 wherein said carrier is sheet-like and said connection region is located at an edge or a center of said carrier.

3. The carrier as claimed in claim 1 wherein said carrier body is a hollow cone, said connection zone being located at the top of said cone.

4. The carrier of claim 3 wherein the axis of each of the cones is collinear.

5. The carrier of claim 1, further comprising:

and the supporting part is arranged between the bearing areas on two adjacent bearing bodies.

6. The carrier of claim 1, further comprising:

the first plate body and the second plate body are arranged in parallel at intervals, and the bearing body is located between the first plate body and the second plate body.

7. The carrier as claimed in claim 6, wherein the first plate has a first hole extending therethrough in a thickness direction of the first plate, and the second plate has a second hole extending therethrough in a thickness direction of the second plate.

8. The carrier of claim 1, wherein the carrier body comprises at least one of a honeycomb structure, a pore-like structure, and a lattice structure; and/or

The supporting body is provided with a through hole penetrating along the thickness direction of the supporting body; and/or

The supporting body is circular, oval or polygonal; and/or

The surface of the bearing area on the bearing body is provided with a microstructure, and the microstructure comprises at least one of a strip-shaped protrusion, a strip-shaped groove and a granular protrusion; and/or

A net structure is arranged between every two adjacent bearing bodies.

9. A bioreactor, comprising:

the vector of any one of claims 1-8;

the jar body, the jar has feed inlet and discharge gate on the body, the carrier set up in the jar body.

10. The bioreactor of claim 9, further comprising:

the stirrer comprises a stirring shaft and stirring blades, wherein the stirring blades are arranged on the stirring shaft, one end of the stirring shaft extends into the tank body, and the stirring blades are positioned in the tank body.

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