CN215050233U - High-efficient fermentation preparation system of ocean rhodotorula - Google Patents
High-efficient fermentation preparation system of ocean rhodotorula Download PDFInfo
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- CN215050233U CN215050233U CN202120466707.7U CN202120466707U CN215050233U CN 215050233 U CN215050233 U CN 215050233U CN 202120466707 U CN202120466707 U CN 202120466707U CN 215050233 U CN215050233 U CN 215050233U
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Abstract
The utility model discloses a high-efficient fermentation preparation system of ocean rhodotorula, belongs to the technical field of cell culture, including the fermentation chamber with install the rotatory culture apparatus in the fermentation chamber, rotatory culture apparatus includes rotation axis and the rotatory thing platform of putting that is used for placing the culture medium, the rotation axis is installed in the fermentation chamber and can rotate along its axis direction, the rotation axis surface encircles and is wrapped up and has a second rotation layer, more than two rotatory thing platforms are installed along the rotation axis direction on the surface of the second rotation layer; and an inoculation pipeline for injecting strains into the culture medium is further arranged in the second rotating layer. The utility model has the advantages that: set up the inoculation pipeline, can utilize tools such as needle tubing will inoculate or the replenishment of nutrient solution after the sterilization, it is more convenient, effective and accurate to this device bactericidal effect is better, and is more abundant even, fine improvement work efficiency and production efficiency.
Description
Technical Field
The utility model relates to a cell culture technical field particularly, relates to a high-efficient fermentation preparation system of ocean rhodotorula.
Background
Rhodotorula benthica is a yeast widely distributed in nature, is rich in various nutrient substances such as protein, glycogen and the like, and produces various active substances including various active substances such as carotenoid, astaxanthin, vitamin and the like. The current devices on the market for fermentation of the marine rhodotorula include automatic fermenters and the like. Automatic fermentation cylinder contains the effect of sterilization and fermentation more, but the most sterilization effect of automatic fermentation cylinder on the market is relatively poor, and inconvenient constant temperature and isopiestic pressure that keeps the fermentation cylinder inside to and it is not convenient enough to inoculate, also can not carry out the nutrient solution at any time and supply, so that cell culture's speed is slower, reduces work efficiency, influences production efficiency.
The applicant researches the defects in the prior art and develops the scheme.
SUMMERY OF THE UTILITY MODEL
Aiming at the problems that the inoculation and the supplement of nutrient solution are inconvenient in the high-efficiency fermentation device of the rhodotorula benthami in the prior art, and the production efficiency is low and the like are finally caused, the utility model provides a high-efficiency fermentation preparation system of the rhodotorula benthami, which comprises a fermentation chamber and a rotary culture device arranged in the fermentation chamber, wherein the rotary culture device comprises a rotary shaft and a rotary object placing table for placing a culture medium, the rotary shaft is arranged in the fermentation chamber and can rotate along the axis direction of the rotary shaft, the outer surface of the rotary shaft is wrapped with a second rotary layer in a surrounding way, and more than two rotary object placing tables are arranged on the surface of the second rotary layer along the axis direction of the rotary shaft; and an inoculation pipeline for injecting strains into the culture medium is further arranged in the second rotating layer.
Be provided with the inoculation pipeline in ocean rhodotorula fermentation preparation system, this inoculation pipeline sets up in the rotatory in situ of second, neither influence other devices easily and put, it is also more pleasing to the eye, thereby can utilize tools such as needle tubing to inject into the inoculation pipeline and accomplish the inoculation process with ocean rhodotorula after the sterilization, and this inoculation pipeline can also carry out the injection of nutrient solution, compare in prior art, the inoculation of this device is more convenient, effective, can also carry out the nutrient solution according to the demand simultaneously and supply, fine improvement work efficiency, and production efficiency.
Preferably, the fermentation chamber is a drum-shaped tank as a whole, two ends of the rotating shaft are respectively installed on the upper and lower surfaces of the rotating shaft, and one end of the rotating shaft is provided with a servo motor for driving the rotating shaft to rotate.
Preferably, the culture medium on each rotary object placing table is provided with the inoculation pipeline for independently injecting the culture medium.
The inoculation pipeline of annotating the material alone sets up, when carrying out the injection, can be more accurate carry out the inoculation of yeast and the interpolation of nutrient solution, can be accurate add a certain quantity of fungus or nutrient solution to a certain culture medium.
Preferably, the rotary object placing tables are distributed on the surface of the second rotary layer in a left-right staggered manner along the length direction of the rotary shaft.
The staggered distribution is beneficial to the observation of the fermentation process in the fermentation process.
Preferably, an electric heating interlayer is arranged inside the fermentation chamber.
The electric heating interlayer can heat the fermentation chamber and keep the constant temperature.
Preferably, the preparation system further comprises a sterilization device, wherein the sterilization device comprises a steam pipe assembly communicated with the interior of the fermentation chamber and a control switch group used for controlling the steam pipe assembly.
Preferably, the steam pipe assembly includes a high temperature steam pipe and a low temperature steam pipe; the high-temperature steam pipe and the low-temperature steam pipe are provided with corresponding electromagnetic valves, and the electromagnetic valves are electrically connected with the control switch group.
Preferably, the sterilization apparatus further comprises a sterilization lamp installed on an upper surface of an inside of the fermenting chamber.
Preferably, the preparation system further comprises a sewage discharging device, the sewage discharging device comprises a guide plate for guiding sewage and a drain pipe for discharging sewage, the guide plate is provided with a guide groove for guiding the sewage, and the drain pipe is connected with an outlet of the guide groove and is arranged on the lower surface of the fermentation chamber.
Preferably, the guide plate is further provided with vent holes for passing steam and an air guide groove for guiding the direction of air flow.
Preferably, a temperature meter for measuring temperature and a pressure meter for measuring pressure are arranged on the outer side wall of the fermentation chamber.
Preferably, an exhaust valve is installed on the fermentation chamber, and the input end of the exhaust valve is electrically connected with the output end of the control switch group.
Preferably, a heat insulation plate is arranged between the servo motor and the upper surface of the outside of the fermentation chamber.
Has the advantages that:
adopt the utility model discloses technical scheme produces beneficial effect as follows:
(1) the marine rhodotorula fermentation preparation system is internally provided with the inoculation pipeline which is arranged in the second rotating layer, so that the arrangement of other devices is not easily influenced, the appearance is more attractive, the marine rhodotorula can be injected into the inoculation pipeline by using tools such as a needle tube and the like after sterilization so as to complete the inoculation process, and the inoculation pipeline can also inject nutrient solution; the inoculation pipeline of annotating the material alone sets up, when carrying out the injection, is difficult to take place to add the condition of error, can be more accurate carry out the inoculation of yeast and the interpolation of nutrient solution, and accurate adding a certain quantity of fungus or nutrient solution to a certain culture medium.
(2) The device is also provided with a sterilization device for sterilizing the device and the culture medium, the sterilization device is provided with a high-temperature steam pipeline and a low-temperature steam pipeline, so that the high-temperature steam and the low-temperature steam can be introduced into the fermentation chamber, the cell culture medium slowly rotates at a constant speed in the high-temperature gas and the low-temperature gas and fully contacts with the sterilization gas to achieve the effect of uniform sterilization, and in addition, the upper surface in the interior of the fermentation chamber is provided with an additional sterilization lamp for further sterilization, so that the full sterilization of the device and the culture medium is ensured; the arrangement of low-temperature steam can also lead the fermentation chamber to be quickly cooled within a certain time after sterilization; the fermentation chamber is provided with an electric heating interlayer for heating the fermentation chamber and keeping the constant temperature.
(3) This device is provided with waste fitting discharging, and waste fitting discharging includes guide plate and sewage pipes, and the moisture in the guide plate can the air of condensing makes it discharge through the blow off pipe along the guiding gutter to still be provided with the air vent on the guide plate, can not influence inside air current circulation, and still be provided with the air guide groove, can guide the air current direction, the air current distribution in the even device.
(4) This device still is provided with thermometer, manometer, can show according to the data on two tables when the operation, carries out the regulation of fermentation chamber internal pressure and temperature, ensures safety.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a schematic view of a preferred fermentation system of the present invention;
FIG. 2 is a schematic view of a preferred rotary culture apparatus of the present invention;
FIG. 3 is a schematic view of a preferred inoculation line of the present invention;
FIG. 4 is a second schematic view of a preferred inoculation line of the present invention;
FIG. 5 is a schematic view of a preferred rotary object-placing table of the present invention;
fig. 6 is a schematic view of a preferred deflector of the present invention.
In the figure: 1. a fermentation chamber; 2. rotating the culture device; 3. a control switch group; 4. a baffle; 5. an exhaust valve; 6. a heat insulation plate; 11. electrically heating the interlayer; 21. a rotating shaft; 22. rotating the object placing table; 23. a second rotation layer; 24. inoculating the pipeline; 25. a servo motor; 26. a sealing cover; 27. a rubber seal; 31. a high temperature steam pipe; 32. a low temperature steam pipe; 33. an electromagnetic valve; 34. a sterilizing lamp; 41. a drain pipe; 42. a diversion trench; 43. a vent hole; 44. and a gas guide groove.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in fig. 1-2, a high-efficiency fermentation preparation system for rhodotorula benthica comprises a fermentation chamber 1 and a rotary culture device 2 installed in the fermentation chamber 1, wherein the rotary culture device 2 comprises a rotary shaft 21 and a rotary object placing table 22 for placing a culture medium, the rotary shaft 21 is installed in the fermentation chamber 1 and can rotate along the axial direction of the rotary shaft, a second rotary layer 23 is wrapped around the outer surface of the rotary shaft 21, and more than two rotary object placing tables 22 are installed on the surface 23 of the second rotary layer along the axial direction of the rotary shaft 21; the second rotating layer 23 is also provided with an inoculation pipeline 24 for injecting strains into the culture medium.
Be provided with the inoculation pipeline in ocean rhodotorula fermentation preparation system, this inoculation pipeline sets up in the rotatory in situ of second, neither influence other devices easily and put, it is also more pleasing to the eye, thereby can utilize tools such as needle tubing to inject into the inoculation pipeline and accomplish the inoculation process with ocean rhodotorula after the sterilization, and this inoculation pipeline can also carry out the injection of nutrient solution, compare in prior art, the inoculation of this device is more convenient, effective, can also carry out the nutrient solution according to the demand simultaneously and supply, fine improvement work efficiency, and production efficiency.
In a preferred embodiment, the fermentation chamber 1 is formed in a drum shape as a whole, and the rotary shaft 21 is installed at both ends thereof on the upper and lower surfaces thereof, respectively, and is installed at one end thereof with a servo motor 25 for driving the rotation thereof.
As shown in FIGS. 2-5, in a preferred embodiment, each rotating platform 22 has a separate feed inoculation line 24 for the medium.
Specifically, in this embodiment, the inoculation pipe 24 may be a thin flexible pipe installed in the second rotating layer 23, the length direction of the thin flexible pipe is set along the axis direction of the rotating shaft, one end of the inoculation pipe 24 is fixed to the rotating object placing table 22, and the inlet of the inoculation pipe 24 at the other end is connected to the outside of the fermentation chamber 1 for inoculating yeast or injecting nutrient solution.
As shown in figure 3, as a preferred embodiment, the inlet of the inoculation duct 24 is provided with a rubber seal 27.
As shown in figures 1-2, as a preferred embodiment, the inoculation line inlet is provided with a removable sealing cap 26. The sealing cover can be arranged at the entrance of the inoculation pipeline in a clamping way, a screwing way and the like.
When the rhodotorula benthica is inoculated, the rhodotorula benthica can be injected by using a needle tube, and the rubber sealing piece can ensure the airtightness of the interior of the fermentation chamber; the sealing cover is arranged, so that the cleanness and the sealing performance of the injection environment can be further guaranteed. The inoculation pipeline of annotating the material alone sets up, when carrying out the injection, can be more accurate carry out the inoculation of yeast and the interpolation of nutrient solution, and be accurate to certain culture medium and add certain quantity of fungus or nutrient solution.
In a preferred embodiment, the fermentation chamber 1 is provided with an observation window for observation.
As shown in fig. 1, the rotary stages 22 are preferably arranged on the surface of the second rotary layer 23 in a staggered manner in the longitudinal direction of the rotary shaft 21.
The staggered distribution is beneficial to observing the fermentation process.
In a preferred embodiment, the fermentation chamber 1 is provided with an electrically heated sandwich 11.
The electric heating interlayer can heat the fermentation chamber and keep the constant temperature.
As a preferred embodiment, the preparation system further comprises a sterilization device comprising a steam pipe assembly communicating with the inside of the fermenting chamber 1 and a control switch group 3 for controlling the steam pipe assembly.
As a preferred embodiment, the steam pipe assembly includes a high temperature steam pipe 31 and a low temperature steam pipe 32; the high temperature steam pipe 31 and the low temperature steam pipe 32 are provided with corresponding electromagnetic valves 33, and the electromagnetic valves are electrically connected with the control switch group 3.
In a preferred embodiment, the steam pipe assemblies include two sets of high temperature steam pipes 31 and low temperature steam pipes 32, the high temperature steam pipes 31 and the low temperature steam pipes 32 form one set, and the two sets of steam pipe assemblies are uniformly distributed at the left and right ends of the fermentation chamber 1.
In a preferred embodiment, a sealing member (not shown) is installed on the steam pipe assembly at a position where the steam pipe assembly is connected to the fermenting chamber 1.
In a preferred embodiment, one or more of the low temperature steam pipe 32 and the high temperature steam pipe 31 may be connected to a humidifier, and preferably, the humidity of the humidifier is controlled to be constant.
As a preferred embodiment, the sterilization apparatus further comprises a sterilization lamp 34 installed on the inner upper surface of the fermenting chamber 1.
The device is provided with a sterilization device for sterilizing the device and the culture medium, the sterilization device is provided with a high-temperature steam pipeline and a low-temperature steam pipeline, so that the high-temperature steam and the low-temperature steam can be introduced into the fermentation chamber, the cell culture medium slowly rotates at a constant speed in the high-temperature gas and the low-temperature gas and fully contacts with the sterilization gas to achieve the uniform sterilization effect, and in addition, the upper surface in the fermentation chamber is additionally provided with a sterilization lamp for further sterilization, so that the full sterilization of the device and the culture medium is ensured; the arrangement of the low-temperature steam can also lead the fermentation chamber to be rapidly cooled within a certain time after the sterilization.
As shown in fig. 1 and 6, as a preferred embodiment, the preparation system further comprises a sewage draining device, the sewage draining device comprises a guide plate 4 for guiding sewage and a drain pipe 41 for draining sewage, the guide plate 4 is provided with a guide groove 42 for guiding sewage, and the drain pipe 41 is connected with an outlet of the guide groove 42 and is installed on the lower surface of the fermentation chamber 1.
In a preferred embodiment, the baffle plate 4 is further provided with a vent hole 43 for passing steam and an air guide groove 44 for guiding the direction of air flow.
In a preferred embodiment, a thermometer (not shown) for measuring temperature and a pressure gauge (not shown) for measuring pressure are disposed on the outer side wall of the fermentation chamber 1.
In a preferred embodiment, the fermentation chamber 1 is provided with an exhaust valve 5, and the input end of the exhaust valve 5 is electrically connected with the output end of the control switch group 3.
As shown in FIG. 1, as a preferred embodiment, a heat insulation plate 6 is provided between the servo motor 25 and the outer upper surface of the fermenting chamber 1.
It should be noted that the electric heating interlayer 11, the exhaust valve 5, the electromagnetic valve 33, the control switch group 3, and the sterilizing lamp 34 described in the present embodiment are all the prior art, and will not be described in detail.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (13)
1. The efficient fermentation and preparation system for the rhodotorula benthica is characterized by comprising a fermentation chamber and a rotary culture device arranged in the fermentation chamber, wherein the rotary culture device comprises a rotating shaft and a rotary object placing table used for placing a culture medium, the rotating shaft is arranged in the fermentation chamber and can rotate along the axis direction of the rotating shaft, a second rotating layer is wrapped around the outer surface of the rotating shaft, and more than two rotary object placing tables are arranged on the surface of the second rotating layer along the axis direction of the rotating shaft; and an inoculation pipeline for injecting strains into the culture medium is further arranged in the second rotating layer.
2. The system for efficiently fermenting and preparing rhodotorula rubra according to claim 1, wherein the fermentation chamber is drum-shaped as a whole, two ends of the rotating shaft are respectively installed on the upper and lower surfaces of the rotating shaft, and one end of the rotating shaft is provided with a servo motor for driving the rotating shaft to rotate.
3. The efficient fermentation preparation system for rhodotorula benthica according to claim 2, wherein said culture medium on each said rotary platform is equipped with said inoculation pipeline for injecting material separately.
4. The efficient fermentation and preparation system for rhodotorula benthica according to claim 3, wherein the rotary shelves are distributed on the surface of the second rotary layer along the length direction of the rotary shaft in a left-right staggered manner.
5. The efficient fermentation preparation system for rhodotorula benthica according to claim 1, wherein an electric heating interlayer is arranged inside the fermentation chamber.
6. The efficient fermentation preparation system for rhodotorula marinus according to claim 1, wherein the preparation system further comprises a sterilization device, the sterilization device comprises a steam pipe assembly communicated with the inside of the fermentation chamber and a control switch group for controlling the steam pipe assembly.
7. The efficient fermentation preparation system for rhodotorula marinus according to claim 6, wherein the steam pipe assembly comprises a high temperature steam pipe and a low temperature steam pipe; the high-temperature steam pipe and the low-temperature steam pipe are provided with corresponding electromagnetic valves, and the electromagnetic valves are electrically connected with the control switch group.
8. The efficient fermentation preparation system for rhodotorula benthica according to claim 7, wherein said sterilization device further comprises a sterilization lamp installed on the upper surface of the inside of the fermentation chamber.
9. The efficient fermentation preparation system for rhodotorula benthica according to claim 8, wherein the preparation system further comprises a sewage draining device, the sewage draining device comprises a guide plate for guiding sewage and a drain pipe for draining sewage, the guide plate is provided with a guide groove for guiding sewage, and the drain pipe is connected with the outlet of the guide groove and is arranged on the lower surface of the fermentation chamber.
10. The efficient rhodotorula benthica fermentation preparation system according to claim 9, wherein the guide plate is further provided with vent holes for passing steam and air guide grooves for guiding air flow direction.
11. The efficient fermentation preparation system for rhodotorula benthica according to claim 6, wherein a temperature gauge for measuring temperature and a pressure gauge for measuring pressure are arranged on the outer side wall of the fermentation chamber.
12. The efficient fermentation preparation system for rhodotorula benthica according to claim 11, wherein an exhaust valve is installed on the fermentation chamber, and the input end of the exhaust valve is electrically connected with the output end of the control switch group.
13. The efficient fermentation preparation system for rhodotorula benthica according to claim 2, wherein a heat insulation plate is arranged between the servo motor and the upper surface of the outside of the fermentation chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120466707.7U CN215050233U (en) | 2021-03-04 | 2021-03-04 | High-efficient fermentation preparation system of ocean rhodotorula |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120466707.7U CN215050233U (en) | 2021-03-04 | 2021-03-04 | High-efficient fermentation preparation system of ocean rhodotorula |
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| CN215050233U true CN215050233U (en) | 2021-12-07 |
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| CN202120466707.7U Active CN215050233U (en) | 2021-03-04 | 2021-03-04 | High-efficient fermentation preparation system of ocean rhodotorula |
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- 2021-03-04 CN CN202120466707.7U patent/CN215050233U/en active Active
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