CN215013015U - Clinical tissue collection activity preservation cryopreservation kit - Google Patents

Abstract

The utility model discloses a clinical tissue collection activity preservation cryopreservation kit, belonging to the technical field of tissue collection and cryopreservation kits; the freezing storage box comprises a box body and a cover body, wherein the cover body covers the box body, and a sampling tube, a freezing storage assembly and a cleaning assembly are fixedly arranged in the box body; the freezing component comprises a freezing liquid storage bottle and at least 1 freezing pipe in number; the cleaning assembly comprises a first cleaning pipe and at least 1 second cleaning pipe. The kit comprises all containers and sterile instruments required in the tissue collection and cryopreservation processes; compared with the prior art, the tissue sample collection range is wider, the operation steps are further simplified, and the trouble of searching for external instruments and containers is reduced.

Description

Clinical tissue collection activity preservation cryopreservation kit

Technical Field

The utility model belongs to the technical field of the kit, especially a clinical tissue gathers active maintenance cryopreserved kit.

Background

The low-temperature cryopreservation is a method for storing collected animal and plant tissues at a specific temperature below zero, and the metabolism and growth of the animal and plant tissues are almost completely stopped by the storage mode, so that the change of the genetic characters of cells is avoided, and the cells and the tissues are prevented from losing the original forms. Animal and plant tissues are generally frozen at low temperature by adopting a special freezing kit.

Most of the existing kits for freezing and storing animal and plant tissues comprise a storage and collection bottle, a freezing and storage tube, a freezing and storage reagent bottle and a cleaning bottle; the collecting bottle is an aseptic bottle, and the cleaning bottle is used for cleaning animal and plant tissues; the freezing tube is used for placing the cleaned animal and plant tissues and preserving the animal and plant tissues by using freezing liquid in the freezing reagent bottle, and the kit can directly freeze the tissues and recover the frozen tissues to obtain cells.

For example, chinese granted patent CN202738683U provides a tissue cryopreservation solution kit, in which a fixed plate inside a box body is provided with a collection bottle and a cryopreservation tube, and further comprises reagent bottles respectively containing a cryopreservation solution and a cleaning solution. The kit can be directly used, and can be cleaned and frozen in time after tissue collection.

For example, chinese granted patent CN211689096U provides a kit for isolating and expanding cultured umbilical cord mesenchymal stem cells, which comprises 9 independent functional areas, and further comprises disposable sterile scissors, disposable sterile forceps, disposable sterile plastic cup and disposable sterile cell filter screen.

For another example, chinese granted patent CN208414421U provides a PRP and adipose-derived stem cell separation kit, which includes a centrifuge tube, a pasteur pipette, a freezing tube and a cell sieve, in addition to a conventional diluent bottle, a separation liquid bottle, a washing liquid bottle, a physiological saline bottle, etc.

However, these above-mentioned several kinds of kits or animal and plant tissue can not in time, thoroughly wash after gathering, or also can not effectively retrieve to the waste liquid, or the consumptive material instrument is incomplete, can not satisfy only with a kit can accomplish the whole animal and plant tissue and gather, wash, freeze the demand of depositing the operation.

Disclosure of Invention

In order to solve the problems of the prior art, the utility model provides a clinical tissue collection activity keeps freezing kit, which is realized by the following technical scheme.

The clinical tissue collection activity preservation cryopreservation kit comprises a box body and a cover body, wherein the cover body covers the box body, and a sampling tube, a cryopreservation assembly and a cleaning assembly are fixedly arranged in the box body; the freezing component comprises a freezing liquid storage bottle and at least 1 freezing pipe in number; the cleaning assembly comprises a first cleaning pipe and at least 1 second cleaning pipe.

In the clinical tissue collection activity preservation cryopreservation kit, different cleaning liquids are filled in the first cleaning tube and the second cleaning tube, and the cleaning liquids are used in different cleaning modes (flushing or infiltration cleaning) or cleaning liquids with different concentrations are used for cleaning collected animal and plant tissue samples. For the convenience of distinguishing, the pipe diameter of the first cleaning pipe can be set to be larger than that of the second cleaning pipe, and other modes can be adopted for distinguishing; the setting of second scavenge pipe is no less than 1, and the purpose satisfies different washing modes. The freezing liquid storage bottle is filled with freezing liquid, and the number of the freezing tubes is at least 1, and the freezing tubes are used for freezing, collecting and shearing the obtained animal and plant tissue samples.

The clinical tissue collection activity preservation cryopreservation kit can adopt, but is not limited to the following use methods:

(1) tissue collection: clinically collecting animal and plant tissues (such as tumor tissues) with the volume of more than or equal to 0.5cm multiplied by 0.5cm, and ensuring the activity of the sampled tissues;

(2) temporary preservation: rapidly placing the collected animal and plant tissues into sterile tissue preservation solution, storing at 4 deg.C for more than or equal to 1h, transferring into cells within 6h, and performing cell-to-cell operation;

(3) tissue cleaning: the animal and plant tissues are respectively cleaned by a first cleaning pipe and a second cleaning pipe, for example, the tissues can be put into the first cleaning pipe and the second cleaning pipe for infiltration and cleaning;

(4) trimming tissue: transferring animal and plant tissues into a new sterile cell culture dish (diameter is 10cm) by using sterile forceps, fully shearing the tissues by using sterile scissors (such as ophthalmic scissors), and keeping the tissues moist in the operation process;

(5) adding a freezing stock solution: adding the frozen stock solution into the sheared tissue according to a specific volume ratio (such as 1:3) of the sheared tissue and the frozen stock solution to disperse the sheared tissue and uniformly mixing for later use, wherein a sterile pipettor or pasteur pipette is adopted in the process;

(6) and (3) freezing and storing tubes for subpackage: the tissue fragment suspensions were labeled on different cryopreservation tubes, and 1ml of each was added, and split into aliquots may require the use of sterile pasteur pipettes or 5ml pipette tips.

So far, utilize above-mentioned kit to accomplish the integration process of gathering, washing, partial shipment animal and plant tissue, whole process need not use other instruments basically, convenient and fast. Each kit only collects animal and plant tissues once, a plurality of samples of the animal and plant tissues obtained by single collection and cutting are washed once, and the collected tissue samples have wider range; and (5) carrying out programmed cooling on the subpackaged freezing tubes, and then preserving.

Preferably, a filter screen groove is fixedly arranged in the box body, and a filter screen is placed in the filter screen groove. The purpose of the sieve is to place the animal and plant tissue on the sieve, wash the tissue with the wash solution and filter the wash solution.

More preferably, a waste liquid pipe is fixedly arranged in the box body. The waste liquid pipe is used for storing waste or used cleaning liquid, frozen stock solution and other liquids, or waste animal and plant tissues; or the filter screen can be placed on the waste liquid pipe and then filtered and cleaned by the cleaning liquid.

Further preferably, the vertical downward projection of box body is square, the cryopreserved subassembly is located the arbitrary corner of box body, waste liquid pipe be located with cryopreserved subassembly relative another corner. The waste liquid pipe is filled with waste materials, so that the waste liquid pipe cannot be too close to the freezing pipe and the freezing liquid storage bottle which need to be frozen to avoid polluting the freezing pipe and the freezing liquid. Through freezing the both ends of depositing subassembly and waste liquid pipe setting at the diagonal of square box body, can furthest avoid taking place above-mentioned pollution problem.

Further preferably, a consumable tool assembly is fixedly arranged in the box body; the consumable tool assembly comprises at least one of disposable sterile forceps, disposable sterile scissors and a disposable sterile pipette. Set up the consumptive material instrument in the box body, can further avoid using external apparatus, simplify the operating procedure, also can avoid the not thorough tissue pollution that causes of or model mismatch of outside apparatus disinfection, or damage other parts of kit.

Still further preferably, the disposable sterile pipette is a disposable sterile pasteur pipette.

Further preferably, the box body is composed of a high layer and a low layer which are arranged in a ladder shape, the sampling tube and the freezing storage assembly are fixedly arranged on the high layer, and the cleaning assembly is fixedly arranged on the low layer. Set the box body to two-layer structure of height to inject parts kinds such as pipe, bottle that high-rise and low level placed separately, be on the one hand in order to match the diameter, the length difference of second scavenge pipe, first scavenge pipe, cryopreserved liquid storage bottle, on the other hand be most pipe, bottle part can choose transparent material for use, be convenient for observe the liquid level among sampling tube, the cryopreserved subassembly, the cleaning assembly, and the particular case of animal and plant tissue.

Further preferably, the number of the freezing tubes is 4, and the freezing tubes are distributed around the freezing solution storage bottle; the number of the second cleaning pipes is 2, and the second cleaning pipes are distributed around the first cleaning pipes. As an optimal cleaning mode of the cleaning pipe, the animal and plant tissues can be quickly washed by the first cleaning pipe, then the animal and plant tissues are respectively subjected to short-time infiltration cleaning by the 2 second cleaning pipes, and the animal and plant tissues can be moisturized by the second cleaning pipes in an operation gap. Of course, the cleaning may be performed by other cleaning methods using the cleaning pipe, in addition to the above cleaning method.

Further preferably, the cover body is provided with grooves which are matched with the sampling tube, the cryopreservation assembly and the cleaning assembly in shape and are independent of each other. The effect of recess is when box body and lid, lets in the sampling tube, freezes to deposit the subassembly, wash the first half of subassembly and can imbed the recess, makes sampling tube, freezes to deposit the subassembly, wash the subassembly and can be firmly fixed in the reagent box.

The internal filling of the case and the cover may be a sterile foam or sponge.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model provides a tissue collection cryopreservation kit with more complete functions, which comprises all containers and sterile instruments needed in the tissue collection and cryopreservation processes; compared with the prior art, the tissue sample collected has wider application range, further simplifies the operation steps, reduces the trouble of searching for external instruments and containers additionally, and has more application occasions.

Drawings

Fig. 1 is a schematic perspective view of the kit provided in example 1;

fig. 2 is a schematic perspective view of a cartridge body of the kit provided in example 2;

in the figure: 1. a box body; 2. a cover body; 3. a sampling tube; 4. freezing and storing the liquid in a bottle; 5. freezing and storing the tube; 6. a first cleaning pipe; 7. a second cleaning pipe; 8. a high layer; 9. a lower layer; 10. a filter screen groove; 11. a waste liquid pipe; 12. a consumable tool assembly; 13. and (5) filtering by using a filter screen.

Detailed Description

The technical solutions of the embodiments in this patent will be described clearly and completely with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments, not all embodiments, of this patent. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the patent without making creative efforts, shall fall within the protection scope of the patent.

In the description of this patent, it is noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "top", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the patent and for simplicity in description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the patent. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of this patent, it is noted that, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "communicating" are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. It is to be noted that all the figures are exemplary representations. The meaning of the above terms in this patent may be specifically understood by those of ordinary skill in the art.

The patent is described in further detail below with reference to specific embodiments and with reference to the attached drawings.

Example 1

As shown in fig. 1, the clinical tissue collection activity preservation cryopreservation kit provided by the embodiment includes a kit body 1 and a cover body 2, wherein the cover body 2 covers the kit body 1, and is characterized in that a sampling tube 3, a cryopreservation assembly and a cleaning assembly are fixedly arranged in the kit body 1; the freezing component comprises freezing liquid storage bottles 4 and 4 freezing tubes 5 (all the freezing tubes are not shown in figure 1), and the freezing tubes 5 are distributed around the freezing liquid storage bottles 4; the cleaning assembly comprises a first cleaning pipe 6 and 2 second cleaning pipes 7 (the second cleaning pipes 7 are not all shown in fig. 1), and the second cleaning pipes 7 are distributed around the first cleaning pipe 6.

In the kit provided by this embodiment, different cleaning solutions are filled in the first cleaning tube and the second cleaning tube; the first cleaning pipe is used for respectively carrying out quick washing and quick infiltration cleaning on a plurality of collected samples of animal and plant tissues; then the second cleaning pipe is used for carrying out infiltration cleaning on the sample of the animal and plant tissues and keeping the animal and plant tissues moist; the freezing liquid storage bottle is filled with freezing liquid, and the freezing tubes are arranged into a plurality of freezing tubes and are respectively used for subpackaging and freezing the same animal and plant tissue sample and can also be used for freezing and storing different collected animal and plant tissue samples.

The use method of the kit comprises the following steps:

(1) tissue collection: clinically collecting animal and plant tissues (such as tumor tissues) of not less than 0.5cm × 0.5cm × 0.5cm, and ensuring the activity of the sampled tissues;

(2) temporary preservation: rapidly placing the collected animal and plant tissues into sterile tissue preservation solution, storing at 4 deg.C for more than or equal to 1h, transferring into cells within 6h, and performing cell-to-cell operation;

(3) tissue cleaning: cleaning animal and plant tissues, for example, the tissues can be put into a second cleaning tube for infiltration and cleaning;

(4) trimming tissue: transferring animal and plant tissues into a new sterile cell culture dish (diameter is 10cm) by using sterile forceps, fully shearing the tissues by using sterile scissors (such as ophthalmic scissors), and keeping the tissues moist in the operation process;

(5) adding a freezing stock solution: adding the cut tissue and the frozen liquid into a frozen tube according to a specific volume ratio (such as 1:3) to disperse the cut tissue and uniformly mixing for later use, wherein a sterile pipettor or a pasteur pipette is adopted in the process;

(6) and (3) freezing and storing tubes for subpackage: the tissue fragment suspension was added to 1ml each, which was marked on a different cryopreservation tube. (dispensing may require preparation of sterile pasteur pipettes or 5ml pipettes plus pipette guns);

so far, utilize above-mentioned kit to accomplish the integration process of gathering, washing, partial shipment animal and plant tissue, whole process need not use other instruments basically, convenient and fast. Each kit only collects animal and plant tissues once, a plurality of collected trimmed animal and plant tissue samples are cleaned once, and the collected tissue samples have wider range; and carrying out programmed cooling on the frozen pipes after subpackaging, and then preserving.

And putting the freezing tube which completes the split charging and freezing of the animal and plant tissues into a program cooling box, putting the box in a refrigerator at minus 80 ℃ for one night, and then timely transferring the box into liquid nitrogen for storage.

Example 2

As shown in fig. 2, the clinical tissue harvesting activity preservation cryopreservation kit provided in this embodiment is further optimized based on example 1 as follows:

the box body 1 consists of a high layer 8 and a low layer 9 which are arranged in a step shape, and the vertical downward projection of the box body 1 is square; still fixed filter screen groove 10, waste liquid pipe 11 and the consumptive material tool assembly 12 of being equipped with in the box body 1, 3 filter screens 13 are placed to range upon range of in the filter screen groove 10.

The filter screen is arranged, so that animal and plant tissues can be conveniently placed on the filter screen, and the tissues are cleaned by cleaning fluid; set up the consumptive material instrument in the box body, can further simplify the process of using external instrument less, further simplify the operating procedure, also can avoid the incomplete or model of external instrument disinfection to mismatch the tissue pollution that causes or damage other containers of kit.

The sampling tube 3, the filter screen groove 10 and the freezing component are fixedly arranged on the high layer 8, and the cleaning component, the consumable tool component and the waste liquid tube 11 are fixedly arranged on the low layer; the freezing and storing component is positioned at any corner of the high layer 8, and the waste liquid pipe 11 is positioned at the other corner of the low layer 9 opposite to the freezing and storing component.

Set the box body to two-layer structure of height to pipe, bottle part kind placed high-level and low level carry out special restriction, in order to match second scavenge pipe, first scavenge pipe, cryopreserving liquid storage bottle's diameter, length difference on the one hand, on the other hand is most pipe, bottle part all can choose for use transparent material, be convenient for observe the sampling tube, cryopreserve the liquid level in subassembly, the cleaning assembly, and animal and plant tissue's particular case. With freezing subassembly and waste liquid pipe setting at the both ends of the diagonal of square box body, can separate freezing pipe, freezing liquid storage bottle as far as possible, avoid waste liquid pipe pollution to freeze and deposit pipe and freezing liquid. Consumable tool assemblies include, but are not limited to, disposable sterile forceps, disposable sterile scissors, disposable sterile pasteur pipettes.

The above embodiments describe the implementation of the present invention in detail, however, the present invention is not limited to the specific details of the above embodiments. Within the scope of the claims and the technical idea of the present invention, various simple modifications and changes can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

Claims (9)

1. The clinical tissue collection activity preservation cryopreservation kit comprises a kit body and a cover body, wherein the cover body covers the kit body, and the kit is characterized in that a sampling tube, a cryopreservation assembly and a cleaning assembly are fixedly arranged in the kit body; the freezing component comprises a freezing liquid storage bottle and at least 1 freezing pipe in number; the cleaning assembly comprises a first cleaning pipe and at least 1 second cleaning pipe.

2. The clinical tissue harvesting activity preservation cryopreservation kit as claimed in claim 1, wherein a filter screen groove is further fixedly arranged in the box body, and no less than 1 filter screen is placed in the filter screen groove.

3. The clinical tissue harvesting activity preservation cryopreservation kit according to claim 1 or 2, wherein a waste liquid tube is further fixed in the box body.

4. The clinical tissue harvesting activity preservation cryopreservation kit of claim 3, wherein the vertical downward projection of the box body is square, the cryopreservation assembly is located at any corner of the box body, and the waste tube is located at the other corner opposite to the cryopreservation assembly.

5. The clinical tissue harvesting activity preservation cryopreservation kit of claim 1 or 4, wherein a consumable tool component is further fixedly arranged in the box body; the consumable tool assembly comprises at least one of disposable sterile forceps, disposable sterile scissors and a disposable sterile pipette.

6. The clinical tissue harvesting activity preservation cryopreservation kit of claim 5, wherein the disposable sterile pipette is a disposable sterile pasteur pipette.

7. The clinical tissue harvesting activity preservation cryopreservation kit as claimed in claim 1 or 4, wherein the kit body is composed of a high layer and a low layer which are arranged in a ladder shape, the high layer is fixedly provided with the sampling tube and the cryopreservation component, and the low layer is fixedly provided with the cleaning component.

8. The clinical tissue harvesting activity preservation cryopreservation kit of claim 1, 2, 4 or 6, wherein there are 4 cryopreservation tubes distributed around the cryopreservation solution storage bottle; the number of the second cleaning pipes is 2, and the second cleaning pipes are distributed around the first cleaning pipes.

9. The clinical tissue harvesting activity preservation cryopreservation kit of claim 1, 2, 4 or 6, wherein the cover body is provided with grooves which are matched with the shapes of the sampling tube, the cryopreservation component and the cleaning component and are independent of each other.

Images