CN208414421U - A kind of PRP and fat stem cell separating kit - Google Patents
A kind of PRP and fat stem cell separating kit Download PDFInfo
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- CN208414421U CN208414421U CN201820696230.XU CN201820696230U CN208414421U CN 208414421 U CN208414421 U CN 208414421U CN 201820696230 U CN201820696230 U CN 201820696230U CN 208414421 U CN208414421 U CN 208414421U
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Abstract
The utility model discloses a kind of PRP and fat stem cell separating kit, including box body and box cover, dilution bottle, separating liquid bottle, Washing liquid bottle, normal saline bottle, centrifuge tube, pasteur pipet, cryopreservation tube and cell sieve are equipped in the box body；The box body is divided into left chamber and right chamber, and the left chamber is divided into upper layer and lower layer, and upper layer is equipped with the pedestal with multiple grooves, the dilution bottle, separating liquid bottle, Washing liquid bottle and normal saline bottle bottom of bottle be entrenched in the groove of pedestal respectively；The left chamber bottom is provided with the storage cabinet for placing cell sieve；It is equipped with upper layer storage chamber and lower layer's storage chamber in the right chamber, spongy layer is equipped in the upper layer storage chamber and lower layer's storage chamber, for the centrifuge tube, pasteur pipet and cryovial of different model to be fixed in upper layer storage chamber and lower layer's storage chamber.The kit amount of storage is big, fixing-stable, and reagent needed for separation PRP and fat stem cell and tool have been organized in, so that operation summary.
Description
Technical field
The utility model relates to kit technical field, specially a kind of PRP and fat stem cell separating kit.
Background technique
Currently, fat transfer has been a mature plastic surgery means.Due to continuous to implantation technique in recent years
Research and improvement, have become a widely applied clinical method now, can be used for enlarging the bosom, depressed scar filling, temporo
Portion's recess filling, forehead filling, Face and cheek filling, the filling of apple flesh, the filling of lower chin etc., by the welcome for asking beautiful person.
Mescenchymal stem cell separation method in traditional adipose tissue block comparative maturity, but used in separation process
Reagent needs preparation early period and preparation and process is comparatively laborious, and separation takes a long time and Primary Growth is slow, and fatty group
It is easy to pollute to knit block treatment process.
Kit is the box for holding the chemical reagent such as detection chemical component, medicament residue, viral species, general to cure
Institute, pharmacy corporation use, and the generation of kit is precisely in order to enable experimenter to get rid of heavy preparation of reagents and optimize
Journey, so generally being equipped with corresponding operation instructions in kit, user is not required to specifications or only needs a small amount of optimization
Satisfied result can be obtained.But a kind of existing kit, when in use, internal space planning is poor, leads to storage
It reagent bottle and actually hangs that the quantity not only stored is few and safety is insufficient, is easily damaged, it is also inconvenient when taking, it reduces
Staff's service efficiency, in mobile kit, it is not easy to grab, be very easy to fall off, cause unnecessary trouble, drop
The practicability and functionality that low kit is.
So how to design a kind of PRP and fat stem cell separating kit, becoming us will currently be solved the problems, such as.
Utility model content
The purpose of this utility model is to provide a kind of PRP and fat stem cell separating kit, to solve above-mentioned background
The problems such as amount of storage proposed in technology is few, safety deficiency and using trouble.
To achieve the above object, the utility model provides the following technical solutions:
A kind of PRP and fat stem cell separating kit, including box body and box cover are equipped with dilution bottle extremely in the box body
Few 1, separating liquid bottle at least one, Washing liquid bottle at least one, normal saline bottle at least one, the centrifuge tube 6-10 branch of 15ml,
Centrifuge tube 6-10 branch, the pasteur pipet 3-5 branch of 3ml, the cryopreservation tube 3-5 branch of 1.8ml and cell sieve at least one of 50ml；
The box body is divided into left chamber and right chamber, and the left chamber is divided into upper layer and lower layer, and upper layer is equipped with multiple grooves
Pedestal, the dilution bottle, separating liquid bottle, Washing liquid bottle and normal saline bottle bottom of bottle be entrenched in the groove of pedestal respectively
It is interior；It is provided with fixed plate at the top of the left chamber, the centre of the fixed plate is provided with multiple fixing ports, in the fixing port
Be equipped with elastic card week, the dilution bottle, separating liquid bottle, Washing liquid bottle and normal saline bottle bottleneck be fastened on respectively it is described
In fixing port；
The left chamber bottom is provided with storage cabinet, and the cell sieve is placed in storage cabinet；
It is equipped with upper layer storage chamber and lower layer's storage chamber in the right chamber, is set in the upper layer storage chamber and lower layer's storage chamber
There is spongy layer, for the centrifuge tube, pasteur pipet and cryovial of different model to be fixed on upper layer storage chamber and lower layer's storage chamber
It is interior；Buffer layer is equipped between the upper layer storage chamber and lower layer's storage chamber.
Preferred embodiment, the left chamber and the right chamber and box body nested encryptions；Card is provided at the top of the box cover
Button, the buckle are fixedly connected with box cover.
Preferred embodiment is provided with cat ear, the cat ear and box body nested encryptions on the right side of the box body.
Preferred embodiment, the storage cabinet and box body insertion connect, and are provided with knob at the top of the storage cabinet, the knob and
Storage cabinet is fixedly connected.
Preferred embodiment, the box cover is equipped with right-angled intersection elastic band, for placing reagent kit product specification.
Compared with prior art, the utility model has the beneficial effects that
1, the kit amount of storage is big, fixing-stable, and separation PRP is organized in reagent needed for fat stem cell and tool
It rises, so that operation summary.
2, this kind of PRP and fat stem cell separating kit, are provided with cell sieve, when needing using cell sieve, Yong Huke
To use knob to extract storage cabinet outward out, storage cabinet is opened, cell sieve is further taken out, after being finished, placed cell sieve and enter storage
Hide cabinet, then be pushed home into, this kind design has more excellent convenience, can in the internal storage cell sieve of kit,
Facilitate staff to use, improves the working efficiency of staff and the functionality of reagent box main body, be provided with elastic card
Piece, when placing reagent bottle using kit, elastic card can play the role of fixating reagent bottle, have resiliency, memory
Property, stability, when kit falls or moves, elastic card can be blocked the bottleneck of reagent bottle, and absorb shock
Power and vibration reduce the shaking of reagent bottle, improve stability, the safety of kit, and the design of this kind of elastic card makes
Taking and placing for reagent bottle also becomes more convenient, it is only necessary to which vertical extraction is put into reagent bottle, so that it may easily
It picks and places, improves the practicability of kit, user is facilitated quickly to use, be provided with foam-rubber cushion, in kit work, foam-rubber cushion
Reagent bottle and centrifuge tube can be made more stable when placing, have elasticity is good, reusable, quick peace tear open it is excellent
Point makes the stability of kit and safety be greatly improved, user-friendly, is provided with lower layer's storage chamber, adopts
With the design of the double-deck storeroom, when placing centrifuge tube, user first takes out upper layer storage chamber and foam-rubber cushion, so that it may under
Layer storage chamber places centrifuge tube, has the advantages that quickly use, highly-safe, capacity is big, makes the sky of kit body interior
Between plan more scientific, promote the capacity of kit storage centrifuge tube, can be also used for storing a variety of test tubes, be convenient for work
Make personnel's use, improves the practicability and functionality of reagent box main body, be provided with cat ear, in mobile reagent box main body,
User can hold cat ear, and using cat ear as impetus, so that it may stablize, kit of easily taking, have structure steady
Fixed, convenient to use advantage, keeps reagent box main body more stable when moving, and finger and being bonded for kit are even closer,
The convenience and safety of kit are improved, buckle is provided with, the service life is longer, cost using buckle made of plastics
Relatively cheap and its wearability is preferable, can be with prolonged use, and in kit, storage makes for a long time, and buckle can make box cover
Stability more, prevent from entering miscellaneous dirt inside kit, improve the structural stability of kit entirety, greatly improve
The practicality.
Detailed description of the invention
Fig. 1 is the agent structure schematic diagram of the utility model；
Fig. 2 is the schematic diagram of the section structure of the utility model；
Fig. 3 is the fixed plate structure schematic diagram of the utility model.
In figure: 1 is reagent box main body, and 101 be pedestal, and 102 be bottle body, and 103 be storage cabinet, and 104 be upper layer storage chamber,
105 be foam-rubber cushion, and 106 be lower layer's storage chamber, and 107 be spongy layer, and 108 be centrifuge tube, and 2 be box body, and 3 be left chamber, and 4 be box
Lid, 5 be buckle, and 6 be fixed plate, and 601 be elastic card, and 602 be fixing port, and 7 be cat ear, and 8 be elastic band, and 9 be knob, and 10 are
Right chamber.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe.
A kind of PRP and fat stem cell separating kit, including box body 2 and box cover 4 are equipped with dilution in the box body 2
Bottle at least one, separating liquid bottle at least one, Washing liquid bottle at least one, normal saline bottle at least one, 15ml centrifuge tube 6-10
Branch, the cryopreservation tube 3-5 branch of the centrifuge tube 6-10 branch of 50ml, the pasteur pipet 3-5 branch of 3ml, 1.8ml and cell sieve at least one；
The box body 2 divides for left chamber 3 and right chamber 10, and the left chamber 3 divides for upper layer and lower layer, and upper layer is equipped with multiple
The pedestal 101 of groove, the dilution bottle, separating liquid bottle, Washing liquid bottle and normal saline bottle bottom of bottle be entrenched in pedestal respectively
In 101 groove；The top of the left chamber 3 is provided with fixed plate 6, and the centre of the fixed plate 6 is provided with multiple fixing ports
602,602 inner circumferential of fixing port is equipped with elastic card 601, the dilution bottle, separating liquid bottle, Washing liquid bottle and physiological saline
The bottleneck of bottle is fastened on respectively in the fixing port 602；
3 bottom of left chamber is provided with storage cabinet 103, and the cell sieve is placed in storage cabinet 103；
Upper layer storage chamber 104 and lower layer's storage chamber 106 are equipped in the right chamber 10, the upper layer storage chamber 104 is under
It is equipped with spongy layer 107 in layer storage chamber 106, for the centrifuge tube, pasteur pipet and cryovial of different model to be fixed on upper layer
In storage chamber 104 and lower layer's storage chamber 106；Buffer layer 105 is equipped between the upper layer storage chamber 104 and lower layer's storage chamber 106.
The left chamber 3 and the right chamber 10 and 2 nested encryptions of box body；The top of the box cover 4 is provided with buckle 5,
The buckle 5 is fixedly connected with box cover 4.
The right side of the box body 2 is provided with cat ear 7, the cat ear 7 and 2 nested encryptions of box body.
The storage cabinet 103 is connected with the insertion of box body 2, is provided with knob 9 at the top of the storage cabinet 103, the knob 9 with
Storage cabinet 103 is fixedly connected.
The box cover 4 is equipped with right-angled intersection elastic band 10, for placing reagent kit product specification.
Kit specification:
[name of product]
Adopted name: PRP fat stem cell separating kit
Commercial designations: PRP fat stem cell separating kit
[packing specification]
A dilution 30ml, B separating liquid 25ml, C liquid is cleaning solution 100mL and D liquid be physiological saline 150ml,
15ml centrifuge tube 10,50ml centrifuge tube 6,3ml pasteur pipet 5 and 1.8ml cryopreservation tube 3, hundred hole cells
Sieve 1.
[purposes] using this kit can high-purity, high-recovery isolate blood PRP and fat stem cell.
[application method] this kit is using steps are as follows:
1, PRP is separated: anticoagulation 10-20ml being dispensed into centrifuge tube a, 1200-2200rpm is centrifuged after ten minutes, is inhaled
Take upper plasma and platelet layer into centrifuge tube b；
2, fat stem cell separates: adipose tissue being placed in cell sieve, after being fully ground sieving with syringe handle, is collected
In c pipe, A dilution is added, 1600-2500rpm makes solution be divided into three layers, discards middle layer after being centrifuged 15 minutes, collects
Upper layer is in d pipe, and underlying collection is in e pipe；
3, the concentration of fat stem cell: being added C liquid into e pipe and be resuspended, and B liquid, 2000-2500rpm centrifugation 20 is added
After minute, take middle white cellular layer in f pipe；
4, fat stem cell purifies: D liquid being added into f pipe, mixes, with 1800-2000rpm centrifugation 5-10 minutes, abandons
Go supernatant that precipitating is stayed to hang and (repeat 3 times) again, fat stem cell needed for repeated washing both obtains；
It 5, is gained PRP stem cell after fat stem cell mixes in PRP and f pipe in b pipe；D pipe small particles fat can
It is used as auxiliary material；
6, gained PRP stem cell is more such as in above-mentioned steps, can freeze in liquid nitrogen, in case next time uses.
[storage] is stored at room temperature and transports.
Claims (5)
1. a kind of PRP and fat stem cell separating kit, including box body (2) and box cover (4) and, characterized in that the box body
(2) in be equipped with dilution bottle at least one, separating liquid bottle at least one, Washing liquid bottle at least one, normal saline bottle at least one,
The centrifuge tube 6-10 branch of 15ml, the centrifuge tube 6-10 branch of 50ml, the pasteur pipet 3-5 branch of 3ml, the cryopreservation tube 3-5 branch of 1.8ml with
And cell sieve at least one；
The box body (2) is divided into left chamber (3) and right chamber (10), and the left chamber (3) is divided into upper layer and lower layer, and upper layer is equipped with band
The pedestal (101) of multiple grooves, the dilution bottle, separating liquid bottle, Washing liquid bottle and normal saline bottle bottom of bottle be fitted into respectively
In the groove of pedestal (101)；It is provided with fixed plate (6) at the top of the left chamber (3), the centre of the fixed plate (6) is set
It is equipped with multiple fixing ports (602), fixing port (602) inner circumferential is equipped with elastic card (601), the dilution bottle, separating liquid
The bottleneck of bottle, Washing liquid bottle and normal saline bottle is fastened on respectively in the fixing port (602)；
Left chamber (3) bottom is provided with storage cabinet (103), and the cell sieve is placed in storage cabinet (103)；
Upper layer storage chamber (104) and lower layer's storage chamber (106), the upper layer storage chamber (104) are equipped in the right chamber (10)
Spongy layer (107) are equipped with in lower layer's storage chamber (106), for consolidating the centrifuge tube, pasteur pipet and cryovial of different model
It is scheduled in upper layer storage chamber (104) and lower layer's storage chamber (106)；The upper layer storage chamber (104) and lower layer's storage chamber (106) it
Between be equipped with buffer layer (105).
2. PRP and fat stem cell separating kit according to claim 1, characterized in that the left chamber (3) and described
Right chamber (10) and box body (2) nested encryptions；It is provided with buckle (5) at the top of the box cover (4), buckle (5) and the box cover
(4) it is fixedly connected.
3. PRP and fat stem cell separating kit according to claim 1, characterized in that set on the right side of the box body (2)
It is equipped with cat ear (7), the cat ear (7) and box body (2) nested encryptions.
4. PRP and fat stem cell separating kit according to claim 1, characterized in that the storage cabinet (103) and box
Body (2) insertion connection, storage cabinet (103) top are provided with knob (9), and the knob (9) and storage cabinet (103) are fixed and connected
It connects.
5. PRP and fat stem cell separating kit according to claim 1, characterized in that the box cover (4) is equipped with ten
Word intersects elastic band (8), for placing reagent kit product specification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820696230.XU CN208414421U (en) | 2018-05-11 | 2018-05-11 | A kind of PRP and fat stem cell separating kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820696230.XU CN208414421U (en) | 2018-05-11 | 2018-05-11 | A kind of PRP and fat stem cell separating kit |
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| Publication Number | Publication Date |
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| CN208414421U true CN208414421U (en) | 2019-01-22 |
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| CN201820696230.XU Active CN208414421U (en) | 2018-05-11 | 2018-05-11 | A kind of PRP and fat stem cell separating kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111547354A (en) * | 2020-05-11 | 2020-08-18 | 南京翼飞雪生物科技有限公司 | RNA extraction kit and extraction method |
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2018
- 2018-05-11 CN CN201820696230.XU patent/CN208414421U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111547354A (en) * | 2020-05-11 | 2020-08-18 | 南京翼飞雪生物科技有限公司 | RNA extraction kit and extraction method |
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