CN210445210U

CN210445210U - Plant tissue culture container

Info

Publication number: CN210445210U
Application number: CN201920822957.2U
Authority: CN
Inventors: 李发虎; 刘金泉; 侯佳; 李龙梅; 李明; 李建文
Original assignee: Vocational and Technical College of IMAU
Current assignee: Vocational and Technical College of IMAU
Priority date: 2019-06-03
Filing date: 2019-06-03
Publication date: 2020-05-05
Anticipated expiration: 2029-06-03

Abstract

The utility model discloses a plant tissue culture container, which relates to the technical field of plant tissue culture and solves the problems that distilled water produced in the process of plant tissue culture is not completely treated and is difficult to take out after the culture is finished; the key points of the technical scheme are as follows: the bottle cap comprises a cylinder body and a top cap connected with the top end of the cylinder body, and the top cap is rotatably connected with the inner wall of the cylinder body; a breathable layer is annularly arranged on the inner wall of the cylinder; a water absorbing body is arranged between the breathable layer and the top cover; the bottle body comprises an upper bottle body and a lower bottle body, a water accumulation groove is formed in the inner wall of the bottom end of the upper bottle body, and the side wall of the water accumulation groove and the outer wall of the top end of the lower bottle body are integrally formed; the bottom surface of the bottle body is provided with a culture plate, the culture plate is in contact with the inner wall of the lower bottle body, the top surface of the culture plate is provided with a support, and the support extends to the opening of the upper bottle body along the self axis direction; the effects of improving the plant tissue culture efficiency and reducing the workload of workers are achieved.

Description

Plant tissue culture container

Technical Field

The utility model relates to a plant tissue culture technical field, more specifically says that it relates to a plant tissue culture container.

Background

Plant tissue culture, namely a plant sterile culture technology, also called in vitro culture, is a discipline which utilizes isolated organs (such as roots, stems, leaves, stem tips, flowers, fruits and the like), tissues (such as cambium, epidermis, cortex, medulla cells, endosperm and the like) or cells (such as megaspore, microspore, somatic cells and the like) and protoplast of a plant body to induce callus, adventitious buds and adventitious roots under the artificial conditions of sterility, proper artificial culture medium, temperature and the like according to the theory that plant cells have totipotency.

The culture method of plant tissue culture comprises non-test tube micro-tissue rapid propagation and test tube tissue culture, wherein the test tube tissue culture is to place explants (namely in vitro tissues, organs or cells) in vessels such as a tissue culture bottle and the like for tissue culture under the aseptic condition to obtain tissue culture bottle seedlings; the existing culture container does not completely treat distilled water generated in the process of plant tissue culture, so that the generated distilled water causes pollution to the cultured plants; meanwhile, the plant tissue is difficult to take out after the culture is finished, the plant tissue is easy to damage, the plant tissue culture efficiency is reduced, and the workload of workers is increased.

Therefore, how to design a plant tissue culture container is a problem which is urgently needed to be solved at present.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a plant tissue culture container has the distilled water of handling plant tissue culture process production completely and conveniently takes out the purpose of the plant tissue that the cultivation finishes, reaches the effect that improves plant tissue culture efficiency and alleviate staff's work load.

The above technical purpose of the present invention can be achieved by the following technical solutions: including the bottle and with the bottle lid of bottle grafting, characterized by: the bottle cap comprises a cylinder and a top cover connected with the top end of the cylinder, and the top cover is rotatably connected with the inner wall of the cylinder; a breathable layer is annularly arranged on the inner wall of the cylinder; a water absorbing body is arranged between the breathable layer and the top cover; the bottle body comprises an upper bottle body and a lower bottle body, a water accumulation groove is formed in the inner wall of the bottom end of the upper bottle body, and the side wall of the water accumulation groove and the outer wall of the top end of the lower bottle body are integrally formed; the bottom surface of the bottle body is provided with a culture plate, the culture plate is in contact with the inner wall of the lower bottle body, the top surface of the culture plate is provided with a support, and the support extends to the opening of the upper bottle body along the axis direction.

By adopting the technical scheme, the water absorbing body is convenient to replace by utilizing the cylinder body and the top cover which is rotationally connected with the inner wall of the top end of the cylinder body; the breathable layer is used, so that the water absorbing body can absorb water in the bottle body through the breathable layer conveniently; the water accumulating tank is used for collecting water which is not absorbed by the water absorbing body conveniently and preventing the water which is not absorbed by the water absorbing body from flowing to the culture plate; the upper bottle body and the lower bottle body which are integrally formed are convenient for improving the sealing property of the culture container; the culture plate is used, so that the tissue culture of the plant is facilitated; the bracket is used, so that the culture plate can be conveniently taken out after the plant tissue culture is finished.

The utility model discloses further set up to: and a screen plate is laid on the upper surface of the culture plate.

Through adopting above-mentioned technical scheme, utilize the otter board, be convenient for finish taking out plant tissue at plant tissue culture and observe and when slicing, can be convenient take out whole plant tissue, prevent to damage plant tissue's root system.

The utility model discloses further set up to: the edge of the screen plate is annularly provided with a baffle plate positioned on the upper surface of the screen plate, and the baffle plate is perpendicular to the screen plate.

Through adopting above-mentioned technical scheme, utilize the baffle for the culture plate is separated with lower bottle bottom, prevents lower bottle bottom surface ponding, thereby pollutes and influences the plant tissue of culture plate upper surface.

The utility model discloses further set up to: the upper surface of the screen plate is divided into a plurality of culture tanks.

Through adopting above-mentioned technical scheme, utilize a plurality of culture tanks of partitioned, when being convenient for prevent to carry out a plurality of plant tissue culture simultaneously, the winding of the wrong festival of back plant roots that finishes of cultivateing is in the same place, inconvenient seedling separation to later stage work load has been increased.

The utility model discloses further set up to: the outer wall of the top end of the upper bottle body is annularly provided with a first sealing layer, and the inner wall of the bottom end of the upper bottle body is annularly provided with a second sealing layer.

Through adopting above-mentioned technical scheme, utilize first sealing layer and second sealing layer, be convenient for between bottle and the bottle lid sealed, improved culture container's leakproofness.

The utility model discloses further set up to: the top cap edge encircles and is equipped with the third sealing layer, barrel top inner wall encircles and is equipped with the fourth sealing layer.

Through adopting above-mentioned technical scheme, utilize third sealing layer and fourth sealing layer, be convenient for between top cap and the barrel sealed, prevent that culture container internal environment from communicating with each other through ventilative layer and external environment, improved culture container's leakproofness.

To sum up, the utility model discloses following beneficial effect has: the water absorbing body is convenient to replace by utilizing the cylinder body and the top cover which is rotationally connected with the inner wall of the top end of the cylinder body; the breathable layer is used, so that the water absorbing body can absorb water in the bottle body through the breathable layer conveniently; the water accumulating tank is used for collecting water which is not absorbed by the water absorbing body conveniently and preventing the water which is not absorbed by the water absorbing body from flowing to the culture plate; the upper bottle body and the lower bottle body which are integrally formed are convenient for improving the sealing property of the culture container; the culture plate is used, so that the tissue culture of the plant is facilitated; the bracket is used, so that the culture plate can be conveniently taken out after the plant tissue culture is finished; the mesh plate is used, so that the whole plant tissue can be conveniently taken out when the plant tissue is taken out for observation and slicing after the plant tissue is cultured, and the root system of the plant tissue is prevented from being damaged; utilize a plurality of culture tanks of divided, when being convenient for prevent to carry out a plurality of plant tissue culture simultaneously, the winding that finishes the wrong festival of back plant roots of cultivateing is in the same place, and inconvenient seedling separation to later stage work load has been increased.

Drawings

Fig. 1 is a schematic structural diagram of the whole in the embodiment of the present invention.

In the figure: 11. the bottle body is arranged; 12. discharging the bottle body; 13. a barrel; 14. a top cover; 15. a breathable layer; 16. a water absorbent body; 2. a water accumulation tank; 3. culturing the plate; 31. a support; 32. a screen plate; 33. a baffle plate; 34. a culture tank; 41. a first sealing layer; 42. a second sealing layer; 43. a third sealing layer; 44. and a fourth sealing layer.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

Example (b): a plant tissue culture container is shown in figure 1 and comprises a bottle body and a bottle cap inserted into the bottle body, wherein the bottle cap comprises a cylinder body 13 and a top cover 14 connected with the top end of the cylinder body 13, the top cover 14 is rotatably connected with the inner wall of the cylinder body 13, and the water absorbing body 16 can be conveniently replaced by utilizing the cylinder body 13 and the top cover 14 rotatably connected with the inner wall of the top end of the cylinder body 13. The inner wall of the cylinder body 13 is annularly provided with a breathable layer 15, and the breathable layer 15 is utilized to facilitate the water absorbing body 16 to absorb the water in the bottle body through the breathable layer 15. A water absorption body 16 is arranged between the breathable layer 15 and the top cover 14. In this embodiment, the water absorbent 16 is lime or various substances capable of absorbing moisture in the air, and the top cover 14 can be opened for replacement when the water absorbent is used to a state where the water absorbing capacity is not strong or the water absorption is saturated. The bottle body comprises an upper bottle body 11 and a lower bottle body 12, a water accumulating groove 2 is arranged on the inner wall of the bottom end of the upper bottle body 11, the side wall of the water accumulating groove 2 is integrally formed with the outer wall of the top end of the lower bottle body 12, and the water accumulating groove 2 is utilized to facilitate the collection of water which is not absorbed by the water absorbing body 16 and prevent the water which is not absorbed by the water absorbing body 16 from flowing to the culture plate 3; the upper bottle body 11 and the lower bottle body 12 are integrally formed to facilitate the sealing of the culture container. The bottom surface of the bottle body is provided with a culture plate 3, the culture plate 3 is in contact with the inner wall of the lower bottle body 12, and the culture plate 3 is utilized to facilitate the tissue culture of plants. The top surface of the culture plate 3 is provided with a bracket 31, the bracket 31 extends to the opening of the upper bottle body 11 along the self axis direction, and the culture plate 3 is convenient to take out after the plant tissue culture is finished by utilizing the bracket 31.

As shown in FIG. 1, a mesh plate 32 is laid on the upper surface of the culture plate 3; by utilizing the net plate 32, the whole plant tissue can be conveniently taken out when the plant tissue is taken out for observation and slicing after the plant tissue culture is finished, and the damage to the root system of the plant tissue is prevented.

As shown in fig. 1, a baffle 33 positioned on the upper surface of the screen plate 32 is arranged around the edge of the screen plate 32, and the baffle 33 is arranged perpendicular to the screen plate 32; by using the baffle 33, the culture plate 3 is separated from the bottom of the lower bottle body 12, and water accumulation on the bottom surface of the lower bottle body 12 is prevented, so that plant tissues on the upper surface of the culture plate 3 are polluted and influenced.

As shown in FIG. 1, the upper surface of the mesh plate 32 is partitioned into a plurality of culture tanks 34; utilize a plurality of culture tanks 34 of divided, when being convenient for prevent to carry out a plurality of plant tissue culture simultaneously, the winding that finishes the wrong festival of back plant roots of cultivateing is together inconvenient to divide the seedling to later stage work load has been increased.

As shown in fig. 1, a first sealing layer 41 is annularly arranged on the outer wall of the top end of the upper bottle body 11, and a second sealing layer 42 is annularly arranged on the inner wall of the bottom end of the cylinder body 13; by using the first sealing layer 41 and the second sealing layer 42, the sealing between the bottle body and the bottle cap is facilitated, and the sealing performance of the culture container is improved.

As shown in fig. 1, the edge of the top cover 14 is provided with a third sealing layer 43, and the inner wall of the top end of the cylinder 13 is provided with a fourth sealing layer 44; by utilizing the third sealing layer 43 and the fourth sealing layer 44, the sealing between the top cover 14 and the cylinder body 13 is facilitated, the internal environment of the culture container is prevented from being communicated with the external environment through the ventilation layer 15, and the sealing performance of the culture container is improved. In the embodiment, the sealing layers are all made of rubber bodies, so that the sealing performance of the container is ensured, and the service life of the sealing layers is prolonged.

The working principle is as follows: the replacement of the water absorption body 16 is facilitated by the cylinder body 13 and the top cover 14 which is rotationally connected with the inner wall of the top end of the cylinder body 13; the breathable layer 15 is used for facilitating the water absorbing body 16 to absorb the water in the bottle body through the breathable layer 15; the water collecting tank 2 is used for conveniently collecting the water which is not absorbed by the water absorbing body 16 and preventing the water which is not absorbed by the water absorbing body 16 from flowing to the culture plate 3; the upper bottle body 11 and the lower bottle body 12 which are integrally formed are convenient for improving the sealing performance of the culture container; the culture plate 3 is used for facilitating the tissue culture of plants; the bracket 31 is used for facilitating the taking out of the culture plate 3 after the plant tissue culture is finished.

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications to the present embodiment without inventive contribution as required after reading the present specification, but all of them are protected by patent laws within the scope of the claims of the present invention.

Claims

1. The utility model provides a plant tissue culture container, includes bottle and the bottle lid of pegging graft with the bottle, characterized by: the bottle cap comprises a cylinder body (13) and a top cover (14) connected with the top end of the cylinder body (13), and the top cover (14) is rotatably connected with the inner wall of the cylinder body (13); the inner wall of the cylinder body (13) is annularly provided with a breathable layer (15); a water absorption body (16) is arranged between the breathable layer (15) and the top cover (14); the bottle body comprises an upper bottle body (11) and a lower bottle body (12), a water accumulating groove (2) is formed in the inner wall of the bottom end of the upper bottle body (11), and the side wall of the water accumulating groove (2) and the outer wall of the top end of the lower bottle body (12) are integrally formed; the bottom surface of the bottle body is provided with a culture plate (3), the culture plate (3) is in contact with the inner wall of the lower bottle body (12), the top surface of the culture plate (3) is provided with a support (31), and the support (31) extends to the opening of the upper bottle body (11) along the self axis direction.

2. The plant tissue culture container according to claim 1, wherein: and a screen plate (32) is paved on the upper surface of the culture plate (3).

3. A plant tissue culture container according to claim 2, wherein: the edge of the screen plate (32) is annularly provided with a baffle (33) positioned on the upper surface of the screen plate (32), and the baffle (33) is vertical to the screen plate (32).

4. A plant tissue culture container according to claim 2, wherein: the upper surface of the mesh plate (32) is divided into a plurality of culture tanks (34).

5. The plant tissue culture container according to claim 1, wherein: go up bottle (11) top outer wall ring and be equipped with first sealing layer (41), barrel (13) bottom inner wall ring is equipped with second sealing layer (42).

6. The plant tissue culture container according to claim 1, wherein: the edge of the top cover (14) is annularly provided with a third sealing layer (43), and the inner wall of the top end of the cylinder body (13) is annularly provided with a fourth sealing layer (44).