CN214830409U - Cell culture and harvesting device - Google Patents
Cell culture and harvesting device Download PDFInfo
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- CN214830409U CN214830409U CN202121708860.2U CN202121708860U CN214830409U CN 214830409 U CN214830409 U CN 214830409U CN 202121708860 U CN202121708860 U CN 202121708860U CN 214830409 U CN214830409 U CN 214830409U
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Abstract
A cell culture and harvest device, which comprises a transfer barrel and a cell collecting barrel, the bottom of bioreactor communicates with the first output tube, install the flow valve on the first output tube, the end of first output tube communicates with the transfer bucket, the bottom of transfer bucket sets up first liquid outlet, first liquid outlet department installs first filter screen, first liquid outlet passes through the second output tube and communicates with the cell collecting vessel, the first break-make valve of mid-mounting of second output tube, one side of transfer bucket passes through the third output tube and communicates with the result bucket, the bottom of cell collecting vessel sets up the fourth output tube, install the second break-make valve on the fourth output tube, a side upper portion of cell collecting vessel sets up the second liquid outlet, second liquid outlet department installs the second filter screen, the second liquid outlet passes through fifth output tube and communicates with the solution supplementing vessel, a side lower part of solution supplementing vessel passes through the sixth output tube and communicates with bioreactor. The application provides a cell culture and harvesting device, which can change liquid and separate products of cells while culturing the cells.
Description
Technical Field
The utility model relates to a cell culture technical field, concretely relates to cell culture and harvesting apparatus.
Background
At present, in cell culture, small-dose culture is mainly carried out in a micro-perforated plate, a plate and a culture bottle or large-scale culture is carried out in a bioreactor, but cell culture and harvesting are two disjointed steps, cell liquid change and product (such as metabolite, exosome and protein expression) separation all need to temporarily or permanently interrupt the cell culture process, and thus the cell culture process is influenced.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems existing in the prior art, the application provides a cell culture and harvesting device which can perform cell liquid change and product separation while cell culture.
In order to realize the technical effect, the utility model discloses a concrete technical scheme as follows:
a cell culture and harvest device comprises a bioreactor, a transfer barrel, a cell collecting barrel, a liquid supplementing barrel and a product barrel, wherein the bottom of the bioreactor is communicated with a first output pipe, a flow valve is installed on the first output pipe, the tail end of the first output pipe is communicated with the transfer barrel, a first liquid outlet is formed in the bottom of the transfer barrel, a first filter screen is installed at the first liquid outlet, the first liquid outlet is communicated with the cell collecting barrel through a second output pipe, a first on-off valve is installed in the middle of the second output pipe, one side of the transfer barrel is communicated with the product barrel through a third output pipe, a fourth output pipe is arranged at the bottom of the cell collecting barrel, a second on-off valve is installed on the fourth output pipe, a second liquid outlet is formed in the upper portion of one side face of the cell collecting barrel, a second filter screen is installed at the second liquid outlet, the second liquid outlet is communicated with the liquid supplementing barrel through a fifth output pipe, and the lower part of one side surface of the liquid supplementing barrel is communicated with the bioreactor through a sixth output pipe.
Further, the bottom of the cell collecting barrel is in an inverted cone shape.
Furthermore, a liquid adding pipe is also arranged on the bioreactor.
The working principle of the cell culture and harvest device is as follows:
the bioreactor is used for large-scale cell culture, the flow valve is used for on-off of the first output pipe and adjustment of the flow, the flow valve is opened to enable a proper amount of cell culture tissues to enter the transfer barrel, the first on-off valve is in a closed state at the moment, the aperture of the first filter screen is set to enable cells to pass but not enable products (such as metabolites, exosomes and protein expressions) to pass, at the moment, the cells passing through the first filter screen are temporarily accumulated in the second output pipe, but the products not passing through the first filter screen enter the product barrel, then opening the first on-off valve to make the cells and liquid passing through the first filter screen enter the cell collecting barrel, repeating the operation, the aperture of the second filter screen is set to be incapable of allowing cells to pass through, so that liquid except the cells can enter the liquid supplementing barrel, and then the liquid can return to the bioreactor through the sixth output pipe; the cells and the liquid in the cell collecting barrel can be discharged through the second on-off valve.
According to the technical scheme, the utility model discloses a set up well commentaries on classics bucket, cell collecting vessel, fluid infusion bucket and result bucket etc. can carry out cell and trade liquid, result separation in cell culture.
Drawings
The present application will be described in further detail below with reference to the accompanying drawings by way of specific embodiments.
Fig. 1 is an overall schematic view of the present invention;
wherein, 1, a bioreactor; 2. a transfer barrel; 3. a cell collection bucket; 4. a liquid replenishing barrel; 5. a product barrel; 6. a first output pipe; 7. a flow valve; 8. a first liquid outlet; 9. a first filter screen; 10. a second output pipe; 11. a first on-off valve; 12. a third output pipe; 13. a fourth output pipe; 14. a second on-off valve; 15. a second liquid outlet; 16. a second filter screen; 17. a fifth output pipe; 18. a sixth output pipe; 19. a liquid feeding pipe.
Detailed Description
In order to make the objects, technical solutions and advantages of the present embodiments more clear, the technical solutions in the present embodiments will be described clearly and completely below with reference to the accompanying drawings in the present embodiments, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without any creative effort belong to the protection scope of the present application.
In the description of the present invention, it is to be understood that the terms "upper end", "lower end", "tail end", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," "large," "small," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, features defined as "first," "second," "large," "small," etc. may explicitly or implicitly include one or more of the features.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral connections; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Examples
Referring to fig. 1, a cell culturing and harvesting apparatus comprises a bioreactor 1, a transfer barrel 2, a cell collecting barrel 3, a fluid infusion barrel 4 and a product barrel 5, wherein the bottom of the bioreactor 1 is communicated with a first output tube 6, a flow valve 7 is installed on the first output tube 6, the tail end of the first output tube 6 is communicated with the transfer barrel 2, a first liquid outlet 8 is arranged at the bottom of the transfer barrel 2, a first filter screen 9 is installed at the first liquid outlet 8, the first liquid outlet 8 is communicated with the cell collecting barrel 3 through a second output tube 10, a first on-off valve 11 is installed in the middle of the second output tube 10, one side of the transfer barrel 2 is communicated with the product barrel 5 through a third output tube 12, a fourth output tube 13 is arranged at the bottom of the cell collecting barrel 3, a second on-off valve 14 is installed on the fourth output tube 13, a second on-off valve 15 is installed at the upper part of one side of the cell collecting barrel 3, a second filter screen 16 is arranged at the second liquid outlet 15, the second liquid outlet 15 is communicated with the liquid supplementing barrel 4 through a fifth output pipe 17, and the lower part of one side surface of the liquid supplementing barrel 4 is communicated with the bioreactor 1 through a sixth output pipe 18.
Wherein, the bottom of the cell collecting barrel 3 is in an inverted cone shape.
Wherein, a liquid adding pipe 19 is also arranged on the bioreactor 1.
The working principle of the utility model is as follows:
the bioreactor 1 is used for large-scale cell culture, the flow valve 7 is used for on-off of the first output pipe 6 and adjusting the flow, the flow valve 7 is opened to enable a proper amount of cell culture tissues to enter the transfer barrel 2, the first on-off valve 10 is in a closed state, the aperture of the first filter screen 9 is set to enable cells to pass through but not enable products (such as metabolites, exosomes and protein expressors) to pass through, at the moment, the cells passing through the first filter screen are temporarily accumulated in the second output pipe 9, but the products which cannot pass through the first filter screen enter the product barrel 5, then the first on-off valve is opened to lead the cells and the liquid which pass through the first filter screen to enter the cell collecting barrel 3, the operation is repeated, the aperture of the second filter screen is set to be unable to let the cells pass through, so the liquid except the cells can enter the liquid replenishing barrel 4, and then the liquid can return to the bioreactor 1 through the sixth output pipe 17; the cells and the liquid in the cell collecting vessel can be discharged through the second on-off valve 13.
It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.
Claims (3)
1. A cell culture and harvest device is characterized by comprising a bioreactor, a transfer barrel, a cell collecting barrel, a liquid supplementing barrel and a product barrel, wherein the bottom of the bioreactor is communicated with a first output pipe, a flow valve is installed on the first output pipe, the tail end of the first output pipe is communicated with the transfer barrel, a first liquid outlet is formed in the bottom of the transfer barrel, a first filter screen is installed at the first liquid outlet, the first liquid outlet is communicated with the cell collecting barrel through a second output pipe, a first on-off valve is installed in the middle of the second output pipe, one side of the transfer barrel is communicated with the product barrel through a third output pipe, a fourth output pipe is arranged at the bottom of the cell collecting barrel, a second on-off valve is installed on the fourth output pipe, a second liquid outlet is formed in the upper portion of one side face of the cell collecting barrel, and a second filter screen is installed at the second liquid outlet, and the second liquid outlet is communicated with the liquid supplementing barrel through a fifth output pipe, and the lower part of one side surface of the liquid supplementing barrel is communicated with the bioreactor through a sixth output pipe.
2. A cell culturing and harvesting device according to claim 1, wherein the bottom of the cell collection vessel is inverted cone-shaped.
3. A cell culture and harvesting device according to claim 1, wherein the bioreactor is further provided with a filler tube.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202121708860.2U CN214830409U (en) | 2021-07-27 | 2021-07-27 | Cell culture and harvesting device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202121708860.2U CN214830409U (en) | 2021-07-27 | 2021-07-27 | Cell culture and harvesting device |
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CN214830409U true CN214830409U (en) | 2021-11-23 |
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CN202121708860.2U Active CN214830409U (en) | 2021-07-27 | 2021-07-27 | Cell culture and harvesting device |
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2021
- 2021-07-27 CN CN202121708860.2U patent/CN214830409U/en active Active
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