CN216972499U - Liquid feeding mechanism for production of temperature-sensitive cell culture apparatus - Google Patents
Liquid feeding mechanism for production of temperature-sensitive cell culture apparatus Download PDFInfo
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- CN216972499U CN216972499U CN202220715072.4U CN202220715072U CN216972499U CN 216972499 U CN216972499 U CN 216972499U CN 202220715072 U CN202220715072 U CN 202220715072U CN 216972499 U CN216972499 U CN 216972499U
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Abstract
The utility model discloses a liquid adding mechanism for production of a temperature-sensitive cell culture apparatus, which relates to the technical field of biological medicines and comprises a liquid adding barrel, wherein the liquid adding barrel is connected with the feed end of a liquid adding pump through a liquid inlet pipe, the discharge end of the liquid adding pump is connected with one end of a liquid outlet pipe, the other end of the liquid outlet pipe is detachably connected with a liquid adding part, the liquid adding part is arranged on a liquid adding fixing seat, a cavity for placing the temperature-sensitive cell culture apparatus is arranged below the liquid adding fixing seat, and a liquid adding valve is also arranged on the liquid outlet pipe. According to the utility model, the temperature-sensitive response type high polymer material coating solution is transferred into the cell culture instrument through the solution adding barrel, the solution adding part, the solution adding valve and the solution adding pump, the arrangement of the solution adding pump and the solution adding valve ensures the accuracy of the solution adding amount, and the arrangement of the solution adding fixing seat ensures the accuracy of the injection of the temperature-sensitive response type high polymer material coating solution into the cell culture instrument, so that the coating effect is ensured.
Description
Technical Field
The utility model relates to the technical field of biological medicines, in particular to a liquid adding mechanism for producing a temperature-sensitive cell culture apparatus.
Background
The traditional cell recovery methods mainly comprise an enzymolysis method and a mechanical method, but both methods can damage membrane proteins to some extent to cause cell function loss, and therefore, the development of a technology for separating cells from a culture apparatus in a non-enzymolysis and non-mechanical mode is a research hotspot at present. Poly (N-isopropyl acrylamide) (PNIPAM) is a most common temperature-sensitive high polymer material which is relatively easy to prepare, and a cell culture apparatus with temperature response characteristics is prepared by performing modified coating on the cell culture apparatus. Such cell culture devices are mainly realized by grafting or coating a temperature sensitive material onto the surface of the culture device. After the cell culture is finished, the cells are automatically fallen off by changing the temperature, so that the damage to the cells is reduced. According to research and research, only the Japan CellSeed company can realize the commercialization of the temperature-sensitive culture apparatus at present, but the temperature-sensitive cell culture apparatus produced by the CellSeed can realize the controllable desorption and attachment of cells, but also has the problems of complex preparation process, long preparation period, small production quantity, poor use stability, small application range of base materials and the like. Therefore, it is very necessary to specially design a mass production device of temperature-sensitive cell culture apparatus to meet the market demand, wherein it is very necessary to design a special liquid feeding mechanism for producing temperature-sensitive cell culture apparatus.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a liquid adding mechanism for producing a temperature-sensitive cell culture apparatus, which is provided with a liquid adding barrel, a liquid adding part, a liquid adding valve and a liquid adding pump, so that temperature-sensitive response type high polymer material coating liquid can be efficiently and accurately injected into the cell culture apparatus, and the coating effect is ensured.
The purpose of the utility model is realized by the following technical scheme:
the utility model provides a production of temperature sensitive cell culture apparatus is with liquid feeding mechanism which characterized in that: including adding the liquid bucket, it links to each other with the feed end of liquid feeding pump through the feed liquor pipe to add the liquid bucket, the discharge end and the drain pipe one end of liquid feeding pump link to each other, the drain pipe other end can be dismantled and be connected with the liquid feeding spare, the liquid feeding spare sets up on the liquid feeding fixing base, liquid feeding fixing base below is provided with the cavity that is used for placing temperature sensitive cell culture apparatus, still be provided with the liquid feeding valve on the drain pipe.
Preferably, the charging valve is arranged on the charging fixing seat.
Preferably, the liquid adding part adopts a funnel type liquid adding part or a single-filling hole type liquid adding part or a multi-filling hole type liquid adding part.
Preferably, the charging valve adopts a pinch valve.
Preferably, the liquid outlet pipe is a silicone tube.
Preferably, a liquid filling opening is arranged above the liquid filling barrel.
Preferably, an oscillator is arranged below the liquid adding barrel.
Preferably, the charging pump adopts a peristaltic pump.
Preferably, the lower end face of the oscillator and the lower end face of the peristaltic pump are located on the same horizontal plane.
The working principle is as follows: temperature-sensitive response type macromolecular material coating liquid adds in adding the liquid bucket, and the setting of oscillator makes the concentration ejection of compact of temperature-sensitive response type macromolecular material coating liquid more even, shifts temperature-sensitive response type macromolecular material coating liquid to the cell culture apparatus in through adding the liquid bucket, liquid feeding spare, liquid feeding valve and liquid feeding pump, and the accuracy of liquid feeding volume is guaranteed in the setting of liquid feeding pump and liquid feeding valve, and the setting of liquid feeding fixing base guarantees that temperature-sensitive response type macromolecular material coating liquid accurately pours into the cell culture apparatus into, guarantees the coating effect.
The beneficial effects of this technical scheme are as follows:
the utility model provides a liquid adding mechanism for producing a temperature-sensitive cell culture instrument.
The utility model provides a liquid feeding mechanism for producing a temperature-sensitive cell culture apparatus, wherein a liquid feeding valve is arranged on a liquid feeding fixing seat, so that the liquid feeding valve is closer to a liquid feeding part, and the accuracy of liquid feeding quantity is ensured.
The utility model provides a liquid feeding mechanism for producing a temperature-sensitive cell culture apparatus, wherein a funnel-shaped liquid feeding part is suitable for single-hole wide-mouth cell culture apparatuses such as cell culture dishes and cell culture sheets; the single-filling hole type liquid adding piece is suitable for cell culture instruments with single-hole narrow openings, such as: cell factories, cell culture flasks, and the like; the multi-filling hole type liquid adding piece is suitable for porous cell culture instruments, such as: 6-well, 12-well, 48-well, 96-well cell culture plates, and the like; the liquid feeding part is detachably connected with the liquid outlet pipe, so that the liquid feeding part is convenient to replace, and the mechanism is suitable for the requirements of the production of various types of cell culture instruments.
The utility model provides a liquid feeding mechanism for producing a temperature-sensitive cell culture apparatus, and the arrangement of the pinch valve and the silicone tube ensures that the action of the liquid feeding valve is more sensitive and the accuracy of liquid feeding amount is ensured.
Drawings
The utility model will be described in further detail with reference to the following description taken in conjunction with the accompanying drawings and detailed description, in which:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural view of a funnel-type liquid adding member according to the present invention;
FIG. 3 is a schematic structural view of a single hole-filling type liquid adding part according to the present invention;
FIG. 4 is a schematic structural view of a multi-hole filling type liquid adding member according to the present invention;
the labels in the figure are: 210. a liquid adding barrel; 211. a liquid filling port; 220. a liquid inlet pipe; 230. a liquid adding pump; 240. a liquid outlet pipe; 250. a liquid adding part; 251. a funnel-shaped liquid feeding piece; 252. a single hole-filling type liquid adding part; 253. a plurality of filling hole type liquid adding pieces; 260. a liquid feeding fixing seat; 270. a liquid charging valve; 280. an oscillator.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
As shown in figures 1-4, a liquid adding mechanism for the production of temperature-sensitive cell culture apparatus is characterized in that: including adding liquid bucket 210, add liquid bucket 210 and link to each other with the feed end of liquid feeding pump 230 through feed liquor pipe 220, the discharge end of liquid feeding pump 230 links to each other with drain pipe 240 one end, the drain pipe 240 other end can be dismantled and be connected with liquid feeding piece 250, liquid feeding piece 250 sets up on liquid feeding fixing base 260, liquid feeding fixing base 260 below is provided with the cavity that is used for placing temperature sensitive cell culture apparatus, still be provided with liquid feeding valve 270 on the drain pipe 240.
Wherein, the liquid adding valve 270 is arranged on the liquid adding fixing seat 260, so that the liquid adding valve 270 is closer to the liquid adding part 250, and the accuracy of the liquid adding amount is ensured.
Wherein, the liquid adding part 250 adopts a funnel type liquid adding part 251, a single filling hole type liquid adding part 252 or a multi-filling hole type liquid adding part 253. The funnel-shaped liquid adding part 251 comprises a liquid adding connecting pipe and a liquid adding funnel, the liquid adding funnel is suitable for single-hole wide-mouth cell culture instruments such as cell culture dishes and cell culture sheets, an internal thread is arranged at the other end of the liquid adding pipe 240, and an external thread matched with the internal thread is arranged on the liquid adding connecting pipe; the single filling hole type liquid feeding member 252 includes a liquid feeding connecting pipe and a plurality of single-hole filling pipes, and the single-hole filling pipes are suitable for cell culture instruments with single-hole narrow openings, such as: the device comprises a cell factory, a cell culture bottle and the like, wherein an internal thread is arranged at the other end of the liquid feeding pipe 240, and an external thread matched with the internal thread is arranged on the liquid feeding connecting pipe; many notes pass liquid feeding spare 253 all includes liquid feeding connecting pipe, liquid separation dish and sets up the liquid feeding nozzle in liquid separation dish below, and many notes pass liquid feeding spare 253 are applicable to porous cell culture apparatus, if: 6-well, 12-well, 48-well, 96-well cell culture plates, and the like; the liquid feeding piece 250 is detachably connected with the liquid outlet pipe 240, so that the liquid feeding piece 250 can be conveniently replaced, and the mechanism is suitable for the production requirements of various cell culture instruments.
Wherein, the charging valve 270 adopts a pinch valve.
Wherein, the liquid outlet pipe 240 adopts a silicone tube. The arrangement of the pinch valve and the silicone tube ensures that the action of the liquid adding valve 270 is more sensitive and the accuracy of the liquid adding quantity is ensured.
A liquid filling port 211 is arranged above the liquid filling barrel 210, and the arrangement of the liquid filling port 211 facilitates the addition of the temperature-sensitive responsive polymer material coating liquid into the liquid filling barrel 210.
Wherein, an oscillator 280 is arranged below the liquid adding barrel 210.
Wherein, the charging pump 230 adopts a peristaltic pump.
Wherein, the lower end face of the oscillator 280 and the lower end face of the peristaltic pump are positioned on the same horizontal plane.
The working principle is as follows: temperature-sensitive response type macromolecular material coating liquid adds in adding liquid bucket 210, the concentration ejection of compact of temperature-sensitive response type macromolecular material coating liquid is more even for the setting of oscillator 280, transfers temperature-sensitive response type macromolecular material coating liquid to the cell culture apparatus in through adding liquid bucket 210, liquid feeding spare 250, liquid feeding valve 270 and liquid feeding pump 230, and the accuracy of liquid feeding volume is guaranteed in the setting of liquid feeding pump 230 and liquid feeding valve 270, and the setting of liquid feeding fixing base 260 guarantees that temperature-sensitive response type macromolecular material coating liquid accurately pours into the cell culture apparatus into, guarantees the coating effect.
In summary, after reading the present disclosure, those skilled in the art can make various other corresponding changes without creative efforts according to the technical solutions and technical concepts of the present disclosure, which all belong to the protection scope of the present disclosure.
Claims (9)
1. The utility model provides a production of temperature sensitive cell culture apparatus is with liquid feeding mechanism which characterized in that: including adding liquid bucket (210), add liquid bucket (210) and link to each other with the feed end of liquid feeding pump (230) through feed liquor pipe (220), the discharge end of liquid feeding pump (230) links to each other with drain pipe (240) one end, drain pipe (240) other end can be dismantled and is connected with liquid feeding spare (250), liquid feeding spare (250) set up on liquid feeding fixing base (260), liquid feeding fixing base (260) below is provided with the cavity that is used for placing temperature sensitive cell culture apparatus, still be provided with liquid feeding valve (270) on drain pipe (240).
2. The liquid adding mechanism for producing the temperature-sensitive cell culture apparatus according to claim 1, wherein: the charging valve (270) is arranged on the charging fixing seat (260).
3. The liquid adding mechanism for producing the temperature-sensitive cell culture apparatus according to claim 2, wherein: the liquid adding part (250) adopts a funnel-shaped liquid adding part (251), a single filling hole-shaped liquid adding part (252) or a multi-filling hole-shaped liquid adding part (253).
4. The liquid adding mechanism for producing the temperature-sensitive cell culture apparatus according to claim 3, wherein: the charging valve (270) adopts a pinch valve.
5. The liquid adding mechanism for producing the temperature-sensitive cell culture apparatus according to claim 4, wherein: the liquid outlet pipe (240) is a silicone tube.
6. The liquid adding mechanism for producing the temperature-sensitive cell culture apparatus according to claim 5, wherein: a liquid filling opening (211) is arranged above the liquid filling barrel (210).
7. The liquid adding mechanism for producing the temperature-sensitive cell culture apparatus according to claim 6, wherein: an oscillator (280) is arranged below the liquid adding barrel (210).
8. The liquid adding mechanism for producing the temperature-sensitive cell culture apparatus according to claim 7, wherein: the charging pump (230) adopts a peristaltic pump.
9. The liquid adding mechanism for producing the temperature-sensitive cell culture apparatus according to claim 8, wherein: the lower end face of the oscillator (280) and the lower end face of the peristaltic pump are located on the same horizontal plane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220715072.4U CN216972499U (en) | 2022-03-30 | 2022-03-30 | Liquid feeding mechanism for production of temperature-sensitive cell culture apparatus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202220715072.4U CN216972499U (en) | 2022-03-30 | 2022-03-30 | Liquid feeding mechanism for production of temperature-sensitive cell culture apparatus |
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| CN216972499U true CN216972499U (en) | 2022-07-15 |
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| CN202220715072.4U Active CN216972499U (en) | 2022-03-30 | 2022-03-30 | Liquid feeding mechanism for production of temperature-sensitive cell culture apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114560284A (en) * | 2022-03-30 | 2022-05-31 | 四川大川合颐生物科技有限公司 | Cell culture apparatus production equipment, intelligent control system and production method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114560284A (en) * | 2022-03-30 | 2022-05-31 | 四川大川合颐生物科技有限公司 | Cell culture apparatus production equipment, intelligent control system and production method thereof |
| CN114560284B (en) * | 2022-03-30 | 2024-04-16 | 四川大川合颐生物科技有限公司 | Cell culture apparatus production equipment, intelligent control system and production method thereof |
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