CN213570534U

CN213570534U - Multilayer cell culture bottle

Info

Publication number: CN213570534U
Application number: CN202021485389.0U
Authority: CN
Inventors: 任宝永
Original assignee: Sige Suzhou Biotechnology Co ltd
Current assignee: Sige Suzhou Biotechnology Co ltd
Priority date: 2020-07-24
Filing date: 2020-07-24
Publication date: 2021-06-29
Anticipated expiration: 2030-07-24

Abstract

The utility model discloses a multilayer cell culture bottle, cultivate room and third culture room including first culture room, second, first culture room top is provided with the third and cultivates the room, the top threaded connection that the room was cultivateed to the third has the top cap, just it cultivates the room to be provided with the second between third culture room and the first culture room and cultivates the room, the room is cultivateed to the second is provided with the multiunit, first culture room, third culture room and multiunit the hasp lock joint in proper order is passed through to the one end that the room was cultivateed to the second, it cultivates the room and the second to be provided with the casing on the bottom face that hasp one end was kept away from to the third, the casing is close to the one end of hasp and is provided with the location arch, just first culture room, third culture room and multiunit all be provided with casing and the recess of the protruding cooperation joint in location on the top. This multilayer cell culture bottle, rational in infrastructure, effectively increased cell culture area in the unit volume, and easy and simple to handle, be worth promoting.

Description

Multilayer cell culture bottle

Technical Field

The utility model belongs to the technical field of the cell culture apparatus, concretely relates to multilayer cell culture bottle.

Background

The cell culture is the core part of biotechnology, and the cell culture bottle is the common device of cell culture, except cancer cell, other cell growth all is adherence growth, because there is glycoprotein outside the cell, has greatly increased the viscidity of cell membrane, therefore can only adherence growth, only one layer of cell, when the whole bottle wall all fills up, the cell stops dividing, gets into G0 phase.

At present, the cell culture bottle of prior art all is a single box body, provides less culture area, therefore need place a plurality of box bodys tiling in proper order on the cupboard shelf when cell batch culture, has occupied a large amount of spaces, and space utilization is inefficient, consequently, is necessary to design a multilayer cell culture bottle.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a multilayer cell culture bottle to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a multilayer cell culture bottle comprises a first culture chamber, a second culture chamber and a third culture chamber, wherein the third culture chamber is arranged above the first culture chamber, the top end of the third culture chamber is in threaded connection with a top cover, a second culture chamber is arranged between the third culture chamber and the first culture chamber, the second culture chamber is provided with a plurality of groups, one ends of the first culture chamber, the third culture chamber and the plurality of groups of second culture chambers are sequentially buckled through buckles, shells are arranged on the bottom end surfaces of the third culture chamber and the second culture chamber, which are far away from one ends of the buckles, a positioning bulge is arranged at one end, close to the buckles, of each shell, and grooves matched and buckled with the shells and the positioning bulge are arranged on the top end surfaces of the first culture chamber, the third culture chamber and the plurality of groups of second culture chambers;

the novel multifunctional incubator is characterized in that a sealing core is arranged in the casing in a sliding mode, a through hole is vertically formed in one side, close to the hasp, of the sealing core, a pull rod is vertically fixed to one end, away from the hasp, of the sealing core, the other end of the pull rod penetrates through the side wall of the casing, is located on the outer side of the casing and is provided with a grab handle, second springs are vertically fixed to the sealing cores on the two sides of the pull rod, one end, away from the sealing core, of each second spring is welded to the side wall of the casing, drainage tubes are formed in the top end wall and the bottom end wall of the casing, and notches and drainage tubes in butt joint with the drainage tubes are formed in the.

Preferably, a bottom plate is horizontally arranged below the first culture chamber, a telescopic positioning rod is vertically fixed on the top end face of the bottom plate, four groups of telescopic ends are arranged on the telescopic positioning rod and fixedly connected with the first culture chamber, a first spring is sleeved outside the telescopic positioning rod, two ends of the first spring are respectively welded with the first culture chamber and the bottom plate, and a vibrator is further arranged on the bottom end face of the first culture chamber.

Preferably, the bottom end faces of the third culture chamber and the second culture chamber are convexly provided with limit strips, and the top end faces of the first culture chamber and the second culture chamber are concavely provided with grooves matched and clamped with the limit strips.

Preferably, the bottom of one side of the first culture chamber is provided with a discharge port, and a valve is arranged on the discharge port.

Preferably, an injection port is formed in the top end face of the top cover, and a cap is connected to the top end of the injection port in a sealing mode.

The utility model discloses a technological effect and advantage: this multilayer cell culture bottle under the cooperation of location arch, spacing and hasp, can add a certain amount of second as required and cultivate the room to increase cell culture's in the unit volume area, so that large batch cell culture, and under the cooperation of sealed core, through-hole, pull rod, second spring, make cell sap flow into in multiunit second culture room and the first culture room that can be simple quick. This multilayer cell culture bottle, rational in infrastructure, effectively increased cell culture area in the unit volume, and easy and simple to handle, be worth promoting.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a cross-sectional view of the present invention;

fig. 3 is a sectional view of the housing of the present invention.

In the figure: 1 bottom plate, 2 first culture chambers, 3 second culture chambers, 4 third culture chambers, 5 top covers, 6 injection ports, 7 limiting strips, 8 telescopic positioning rods, 9 first springs, 10 discharge ports, 11 vibrators, 12 shells, 13 positioning bulges, 14 flow guide pipes, 15 hasps, 16 sealing cores, 17 through holes, 18 drainage pipes, 19 second springs and 20 pull rods.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model provides a multilayer cell culture bottle as shown in figures 1-3, which comprises a first culture chamber 2, a second culture chamber 3 and a third culture chamber 4, a third culture chamber 4 is arranged above the first culture chamber 2, the top end of the third culture chamber 4 is connected with a top cover 5 through screw threads, a second culture chamber 3 is arranged between the third culture chamber 4 and the first culture chamber 2, a plurality of groups of the second culture chambers 3 are arranged, one ends of the first culture chamber 2, the third culture chamber 4 and the plurality of groups of second culture chambers 3 are buckled in sequence through hasps 15, a shell 12 is arranged on the bottom end surface of the third culture chamber 4 and the second culture chamber 3 far away from one end of the buckle 15, a positioning bulge 13 is arranged at one end of the shell 12 close to the buckle 15, grooves matched and clamped with the shell 12 and the positioning bulges 13 are formed in the top end surfaces of the first culture chamber 2, the third culture chamber 4 and the plurality of groups of second culture chambers 3;

a sealing core 16 is arranged in the shell 12 in a sliding manner, a through hole 17 is vertically formed in one side, close to the hasp 15, of the sealing core 16, a pull rod 20 is vertically fixed at one end, far away from the hasp 15, of the sealing core 16, the other end of the pull rod 20 penetrates through the side wall of the shell 12, is located on the outer side of the shell 12 and is provided with a grab handle, second springs 19 are vertically fixed on the sealing cores 16 on two sides of the pull rod 20, one end, far away from the sealing core 16, of each second spring 19 is welded with the side wall of the shell 12, drainage tubes 18 are formed in the top end wall and the bottom end wall of the shell 12, and notches and drainage tubes 14 in butt joint with the drainage tubes 18 are formed in the top end face and the bottom end face of the first culture.

Specifically, the below level of first cultivation room 2 is provided with bottom plate 1, the vertical fixation has flexible locating lever 8 on the face of bottom plate 1 top, flexible locating lever 8 is provided with four groups and flexible end and first cultivation room 2 fixed connection, first spring 9 has been put to flexible locating lever 8 overcoat, the both ends of first spring 9 weld with first cultivation room 2 and bottom plate 1 respectively, still install electromagnetic shaker 11 on the face of the bottom of first cultivation room 2 for the culture solution that will contain the cell tiles fast.

Specifically, the third is cultivateed room 4 and the second and is cultivateed and all convexly be equipped with spacing 7 on the bottom face of room 3, first cultivation room 2 and second are cultivateed and are equipped with the recess with spacing 7 cooperation joint on the top face of room 3, and through spacing 7's setting, the joint is more firm between room 4 is cultivateed to messenger first cultivation room 2, second cultivation room 3 and the third.

Specifically, the bottom of one side of the first culture chamber 2 is provided with a discharge port 10, and a valve is mounted on the discharge port 10 and used for discharging cell culture solution.

Specifically, an injection port 6 is formed in the top end face of the top cover 5, and a cap is connected to the top end of the injection port 6 in a sealing mode and used for adding reagents during cell culture overshoot, so that cell culture pollution caused by frequent opening of the top cover 5 is avoided.

The working principle is as follows: when the multilayer cell culture bottle is used, a certain number of second culture chambers 3 are clamped between a first culture chamber 2 and a third culture chamber 4 according to the cell culture amount, one end of each second culture chamber is clamped by a positioning bulge 13, the other end of each second culture chamber is sequentially buckled by a buckle 15, then culture solution containing cells is injected into the third culture chamber 4 and is sealed by a top cover 5, then a pull rod 20 on one side of a shell 12 is sequentially pulled from high to low, the pull rod 20 drives a sealing core 16 to slide towards one end far away from the buckle 15, when a through hole 17 in the sealing core 16 is overlapped with drainage tubes 18 on the top end and the bottom end of the shell 12, the culture solution containing the cells flows into the second culture chambers 3 from the first culture chamber 2, and the culture solution containing the cells can simply and rapidly flow into the first culture chamber 2 and a plurality of groups of second culture chambers 3, so that the cell culture area in unit volume is effectively increased, so as to be convenient for large-batch cell culture, and when a reagent is required to be added in the cell culture process, liquid can be added through the injection port 6, thereby avoiding the cell culture environment from being polluted due to frequent opening of the top cover 5; finally, through opening the vibrator, under the cooperation of first spring 9 and flexible locating lever 8, can be located first culture chamber 2, second culture chamber 3 and the third culture chamber 4 culture solution that contains the cell and tile rapidly, be favorable to the cell growth. This multilayer cell culture bottle, rational in infrastructure, effectively increased cell culture area in the unit volume, and easy and simple to handle, be worth promoting.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. A multilayer cell culture bottle, includes first culture room (2), second culture room (3) and third culture room (4), its characterized in that: a third culture chamber (4) is arranged above the first culture chamber (2), the top end of the third culture chamber (4) is connected with a top cover (5) by screw thread, a second culture chamber (3) is arranged between the third culture chamber (4) and the first culture chamber (2), the second culture chamber (3) is provided with a plurality of groups, the first culture chamber (2), the third culture chamber (4) and one end of the plurality of groups of second culture chambers (3) are buckled in sequence through hasps (15), a shell (12) is arranged on the bottom end surface of one end of the third culture chamber (4) and the second culture chamber (3) far away from the hasp (15), a positioning bulge (13) is arranged at one end of the shell (12) close to the hasp (15), grooves matched and clamped with the shell (12) and the positioning bulges (13) are formed in the top end surfaces of the first culture chamber (2), the third culture chamber (4) and the plurality of groups of second culture chambers (3);

a sealing core (16) is arranged in the shell (12) in a sliding way, a through hole (17) is vertically arranged on one side of the sealing core (16) close to the hasp (15), a pull rod (20) is vertically fixed at one end of the sealing core (16) far away from the hasp (15), the other end of the pull rod (20) penetrates through the side wall of the shell (12) and is positioned outside the shell (12) and is provided with a grab handle, a second spring (19) is vertically fixed on the sealing core (16) at the two sides of the pull rod (20), one end of the second spring (19) far away from the sealing core (16) is welded with the side wall of the shell (12), and the top end wall and the bottom end wall of the shell (12) are both provided with a drainage tube (18), the top end surface and the bottom end surface of the first culture chamber (2), the second culture chamber (3) and the third culture chamber (4) are respectively provided with a notch and a draft tube (14) which are butted with the draft tube (18).

2. The multi-layered cell culture flask according to claim 1, wherein: the lower level of first cultivation room (2) is provided with bottom plate (1), vertically fixed with flexible locating lever (8) on bottom plate (1) top face, flexible locating lever (8) are provided with four groups and flexible end and first cultivation room (2) fixed connection, first spring (9) have been put to flexible locating lever (8) overcoat, the both ends of first spring (9) weld with first cultivation room (2) and bottom plate (1) respectively, still install electromagnetic shaker (11) on the bottom face of first cultivation room (2).

3. The multi-layered cell culture flask according to claim 1, wherein: the bottom face of room (3) is cultivateed to the third all protruding spacing (7) of being equipped with on the bottom face of room (4) and second, the concave recess that is equipped with spacing (7) cooperation joint on the top face of room (3) is cultivateed to first cultivation room (2) and second.

4. The multi-layered cell culture flask according to claim 1, wherein: the bottom of one side of the first culture chamber (2) is provided with a discharge port (10), and the discharge port (10) is provided with a valve.

5. The multi-layered cell culture flask according to claim 1, wherein: an injection port (6) is formed in the top end face of the top cover (5), and the top end of the injection port (6) is connected with a cap in a sealing mode.