CN214076705U

CN214076705U - Vehicle-mounted positive pressure nucleic acid detection reagent preparation system

Info

Publication number: CN214076705U
Application number: CN202021335992.0U
Authority: CN
Inventors: 谢孝民; 李丹; 谢正华; 熊磊; 陈才夫; 孙祎
Original assignee: Shanghai 3D Medicines Co Ltd
Current assignee: Shanghai 3D Medicines Co Ltd
Priority date: 2020-07-09
Filing date: 2020-07-09
Publication date: 2021-08-31
Anticipated expiration: 2030-07-09

Abstract

The utility model relates to a system is prepared to on-vehicle malleation nucleic acid detect reagent, include the entry buffer module who connects gradually by airtight door mutual isolation, the module is prepared to the reagent, filth processing module and export buffer module, guarantee one-way personnel simultaneously on vehicle platform through the whole establishment of malleation laboratory that will be used for nucleic acid detect reagent to prepare, the airtight access way of article, can be at any time as required quick travel, dispose to the area of needs most in a flexible way, can effectively improve hot area nucleic acid detect reagent configuration ability through reasonable allotment, promote nucleic acid detection efficiency, need not the repeated construction malleation laboratory simultaneously, avoid the wasting of resources.

Description

Vehicle-mounted positive pressure nucleic acid detection reagent preparation system

Technical Field

The utility model relates to a pathogen detection equipment technical field especially relates to a system is prepared to on-vehicle malleation nucleic acid detect reagent.

Background

The nucleic acid amplification detection comprises the preparation of a reaction reagent, sample treatment and amplification, and the reaction reagent is easily polluted by nucleic acid to cause false positive in detection, so that the result is misjudged. Therefore, the reagent preparation room needs to control the air flow direction through pressure difference, and a positive pressure laboratory is used for ensuring that the reagent preparation environment is not polluted by the external environment. For a sudden epidemic situation, a large number of patients need to perform nucleic acid detection in a short time, and at the moment, the number of positive pressure laboratories in a hot spot area is insufficient, the configuration capacity of a nucleic acid detection reagent cannot meet the requirement, and the nucleic acid detection efficiency is reduced.

SUMMERY OF THE UTILITY MODEL

For solving prior art's is not enough, the utility model provides a system is prepared to on-vehicle malleation nucleic acid detect reagent guarantees one-way personnel, the airtight access way of article simultaneously through the whole establishment of malleation laboratory that will be used for nucleic acid detect reagent to prepare on vehicle platform, the nucleic acid detect reagent preparation ability that can the quick travel deployment is provided, can effectively improve hot spot area nucleic acid detect reagent configuration ability through reasonable allotment, promote nucleic acid detection efficiency, need not the repeated construction malleation laboratory simultaneously, avoid the wasting of resources.

In order to achieve the above object, the utility model discloses the technical scheme who adopts includes:

a vehicle-mounted positive pressure nucleic acid detection reagent preparation system is characterized by comprising an inlet buffer module, a reagent preparation module, a dirt disposal module and an outlet buffer module which are mutually isolated and sequentially connected by an airtight door;

the inlet buffer module comprises a first positive pressure device, a first airtight door connected with the reagent preparation module and a second airtight door connected with the external environment;

the reagent preparation module comprises a second positive pressure device, a reagent storage device and a superclean bench for placing a reagent preparation instrument, and the side wall of the reagent preparation module is at least provided with an airtight transfer window connected with the external environment;

the waste disposal module comprises a third positive pressure device, a third airtight door connected with the reagent preparation module and a fourth airtight door connected with the outlet buffer module; the dirt disposal module further comprises at least one airtight transfer window connected to an external environment;

the exit buffer module includes a fourth positive pressure device and a fifth airtight door connected to the external environment.

Further, the stable ambient pressure value that second malleation equipment provided is higher than the stable ambient pressure value that third malleation equipment provided, just the stable ambient pressure value that third malleation equipment provided is higher than the stable ambient pressure value that first malleation equipment with second malleation equipment provided.

Further, the reagent storage apparatus includes a freezing refrigerator of-20 ℃ and a refrigerating refrigerator of 4 ℃.

Further, the reagent preparation module further comprises a storage rack and a buffer chamber storage cabinet arranged on the superclean bench.

Further, the sewage disposal module comprises a hand washing sink, a waste liquid tank, a waste garbage can and a waste terminal treatment device.

Further, the inlet buffer module, the reagent preparation module, the sewage treatment module and the outlet buffer module are also provided with an ultraviolet disinfection lamp and a ventilation device.

Further, an ultraviolet disinfection lamp is arranged in the airtight transfer window.

Further, the inlet buffer module, the reagent preparation module, the sewage disposal module and the outlet buffer module provide power through external power supply; the external power supply comprises a mains supply interface, a generator power supply and/or a photovoltaic power supply arranged on the top of the outer side of the system.

Further, the system also comprises an independent air conditioning device.

The utility model has the advantages that:

adopt on-vehicle malleation nucleic acid detect reagent preparation system has realized establishing the complete malleation laboratory that has entry, export buffer module on vehicle-mounted platform, can accomplish the whole flows of nucleic acid detect reagent configuration in this system, make nucleic acid detect reagent configuration ability break away from the constraint of fixed laboratory, can be at any time as required quick travel, arrange to the area of needs most in a flexible way, make full use of current nucleic acid detect reagent configuration ability, reduce interim malleation laboratory construction pressure, be the ideal solution of the large amount of detection demands of the response emergency.

Drawings

FIG. 1 is a schematic view of the top view of the internal structure of the embodiment of the system for preparing the vehicle-mounted positive pressure nucleic acid detecting reagent of the present invention.

Fig. 2 is a schematic view of the internal bottom structure of the embodiment of the present invention.

Description of the figure numbering: 1-inlet buffer module, 11-first positive pressure device, 12-first airtight door, 13-second airtight door, 2-reagent preparation module, 21-second positive pressure device, 221-freezing refrigerator, 222-refrigerating refrigerator, 23-super clean bench, 24-airtight transfer window, 25-storage rack, 26-buffer room storage cabinet, 3-dirt disposal module, 31-third positive pressure device, 32-third airtight door, 33-fourth airtight door, 4-outlet buffer module, 41-fourth positive pressure device and 42-fifth airtight door.

Detailed Description

For a clearer understanding of the present invention, reference will be made to the following detailed description of the embodiments with reference to the accompanying drawings.

Fig. 1 and fig. 2 show the schematic structural diagram of an embodiment of the present invention, in this embodiment, the inlet buffer module 1, the reagent preparation module 2, the sewage disposal module 3, and the outlet buffer module 4 are sequentially disposed on a chassis of a van vehicle, and the inlet buffer module 1, the reagent preparation module 2, the sewage disposal module 3, and the outlet buffer module 4 are all isolated from the vehicle cab completely and airtightly to avoid pollution, and of course, the system may also be disposed in other suitable movable structures such as a van trailer or a container. The inlet buffer module 1 comprises a first positive pressure device 11 and a first airtight door 12 connected with the reagent preparation module 2 and a second airtight door 13 connected with the external environment; when a worker needs to enter the reagent preparation module 2 to operate, the worker must first pass through the inlet buffer module 1. The reagent preparing module 2 comprises a second positive pressure device 21, a reagent storage device consisting of a freezing refrigerator 221 and a refrigerating refrigerator 222, a clean bench 23 for placing reagent preparing instruments (such as a pipette, a mini centrifuge, a mini-type vortex oscillator), and particularly, the reagent preparing module can also comprise a storage rack for placing equipment and consumables and a buffer chamber storage cabinet 26 arranged on the clean bench 23, and the side wall of the reagent preparing module 2 is at least provided with an airtight transmission window 24 connected with the external environment, and a safe passage for transmitting articles with the outside is formed through the airtight transmission window 24; the reagent dispensing module 2 is preferably further configured with power distribution equipment for electrically connecting mains electricity, generator power, and/or overhead photovoltaic power supplies. The power distribution equipment preferably automatically distributes power supply through the connection condition of commercial power, a generator power supply, roof photovoltaic power supply and/or vehicle engine power supply, ensures stable system power supply, and especially can ensure continuous long-time stable power supply of the reagent storage equipment, so that the reagent storage equipment can continuously work for a long time without limitation. The waste disposal module 3 is provided with at least one airtight transfer window 24 connected to the external environment for safe transfer of waste and the like. In order to ensure that the reagent preparation process is not polluted by the external environment, the inlet buffer module 1, the reagent preparation module 2, the sewage disposal module 3 and the outlet buffer module 4 are respectively provided with independent positive pressure equipment to manufacture a positive pressure environment, preferably, the positive pressure equipment to which each module belongs is respectively arranged at the bottom of the module as shown in fig. 2, and the space in the module is not occupied; the stable ambient pressure value provided by the second positive pressure equipment 21 is higher than the stable ambient pressure value provided by the third positive pressure equipment 31, and the stable ambient pressure value provided by the third positive pressure equipment 31 is higher than the stable ambient pressure values provided by the first positive pressure equipment 11 and the second positive pressure equipment 21, so that at least three-order positive pressure gradient environment is formed, and the influence of external pollution on reagent preparation is completely eradicated; for example, one preferred pressure gradient is set as: the second negative pressure device 21 provides a steady ambient pressure value of 30Pa, the third negative pressure device 31 provides a steady ambient pressure value of 20Pa, and the first negative pressure device 11 and the second negative pressure device 21 provide a steady ambient pressure value of 10 Pa. Meanwhile, the air-tight door connected with each module can be preferably set to be a revolving door structure which is opened by rotating towards the side with a higher positive pressure value, and the pressure difference between the two modules can enable a worker to feel proper damping when opening the air-tight door and enable the air-tight door of the revolving door structure to be closed automatically, so that the air-tight damage caused by forgetting to close the door is avoided. In order to improve the protection effect of the system, ultraviolet disinfection lamps can be preferably arranged in the inlet buffer module 1, the reagent preparation module 2, the dirt treatment module 3, the outlet buffer module 4 and the airtight transfer window 24, so that the vehicle-mounted system can be disinfected and cleaned; the inlet buffer module 1, the reagent preparation module 2, the dirt disposal module 3 and the outlet buffer module 4 are also provided with independent air exchange devices, preferably air exchange devices with filtering functions, so that air in the modules is kept clean; the system is also provided with an independent air conditioning device different from the vehicle-mounted platform, so that pollution caused by a mixed air conditioner is avoided.

When the system of the utility model is used, a worker enters the inlet buffer module 1 through the second airtight door 13, disinfects and wears various protective measures, and then enters the reagent preparation module 2 through the first airtight door 12 to prepare and store a nucleic acid detection reagent; after the work is finished, the waste is required to enter the waste treatment module 3 through the third airtight door 32 to be properly treated, then enters the outlet buffer room through the fourth airtight door 33, finally leaves the system through the fifth airtight door 42, the whole movement line of personnel is in one-way movement, and the safety is guaranteed to the maximum extent. When reagent needs to be transferred to a nucleic acid amplification laboratory or a sample processing laboratory, the airtight transfer window 24 arranged on the side wall of the reagent preparation module 2 is used, the module side window is opened to place the reagent in, the module side window is closed, and after the reagent is sterilized and disinfected by the ultraviolet disinfection lamp in the airtight transfer window 24, outside staff opens the outside window and takes out the detection reagent.

For the entrance buffer module 1, besides providing necessary functions of changing clothes and sterilizing for personnel, a rest function of detecting personnel, such as a washroom, a bathroom and the like, can be preferably arranged by utilizing a vacant position.

On-vehicle malleation nucleic acid detect reagent preparation system both can the exclusive use, also can regard as a part to use with other portable laboratory structure combinations, form the nucleic acid detection laboratory of a complete flow.

The above description is only for the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A vehicle-mounted positive pressure nucleic acid detection reagent preparation system is characterized by comprising an inlet buffer module, a reagent preparation module, a dirt disposal module and an outlet buffer module which are mutually isolated and sequentially connected by an airtight door;

2. The system of claim 1, wherein the second positive pressure device provides a higher steady ambient pressure value than the third positive pressure device, and wherein the third positive pressure device provides a higher steady ambient pressure value than the first and second positive pressure devices.

3. The system of claim 2, wherein the reagent storage device comprises a-20 ℃ freezer refrigerator and a 4 ℃ refrigerator.

4. The system of claim 2, wherein the reagent compounding module further comprises a storage rack and a buffer chamber storage cabinet disposed on the clean bench.

5. The system of claim 2, wherein the waste disposal module comprises a hand wash basin, a waste tank, a waste bin, and a waste terminal disposal device.

6. The system of any one of claims 1 to 5, wherein the inlet buffer module, reagent dispensing module, waste disposal module and outlet buffer module are further provided with a UV disinfection lamp and a ventilation device.

7. The system of any one of claims 1 to 5, wherein an ultraviolet disinfection lamp is further disposed within the hermetic transfer window.

8. The system of any one of claims 1 to 5, wherein the inlet buffer module, reagent dispensing module, waste disposal module, and outlet buffer module are powered by an external power supply; the external power supply comprises a mains supply interface, a generator power supply and/or a photovoltaic power supply arranged on the top of the outer side of the system.

9. The system of any one of claims 1 to 5, further comprising a separate air conditioning unit.