CN213967038U - Antibody separation screening installation - Google Patents
Antibody separation screening installation Download PDFInfo
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- CN213967038U CN213967038U CN202022404141.3U CN202022404141U CN213967038U CN 213967038 U CN213967038 U CN 213967038U CN 202022404141 U CN202022404141 U CN 202022404141U CN 213967038 U CN213967038 U CN 213967038U
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- 238000000926 separation method Methods 0.000 title claims abstract description 64
- 238000012216 screening Methods 0.000 title claims abstract description 60
- 238000009434 installation Methods 0.000 title description 4
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 238000003756 stirring Methods 0.000 claims abstract description 11
- 239000002244 precipitate Substances 0.000 claims abstract description 9
- 238000005342 ion exchange Methods 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 230000008021 deposition Effects 0.000 claims abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000002523 gelfiltration Methods 0.000 claims description 6
- 235000013547 stew Nutrition 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 238000001556 precipitation Methods 0.000 description 10
- 238000004062 sedimentation Methods 0.000 description 7
- 239000013049 sediment Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 125000004122 cyclic group Chemical group 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
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Abstract
The utility model provides an antibody separating and screening device, which comprises a base and a crushing and separating device, and is characterized in that the crushing and separating device is arranged on a bracket, the crushing and separating device comprises a motor, a spiral stirring rod, an ultrasonic generator and a separating and crushing cavity, the motor is arranged on the upper end surface of the separating and crushing cavity, the spiral stirring rod is connected with the output end of the motor, the ultrasonic generator is arranged on the side wall of the separating and crushing cavity, the lower end part of the crushing and separating device is provided with a centrifugal separating device for further separating antibody solution, the lower end of the centrifugal separating device is provided with a deposition and standing cavity, the lower end part of the deposition and standing cavity is provided with a precipitate outlet, the base is also provided with a screening box body, a clear liquid outlet is connected with the screening box body, the inner cavity of the screening box body is provided with a gel filtering and screening layer and an ion exchange and screening layer, the utility model discloses it is showing more to the separation screening effect of antibody solution to compare in traditional equipment.
Description
Technical Field
The utility model relates to a medical field specifically is an antibody separation screening installation.
Background
Antibodies, which are a specific antibody molecule, are used as reagents for in vitro diagnosis, drugs for treating diseases, ligands for immunoaffinity chromatography, and the like. Has wide application in the fields of life science research, biotechnology and medicine. In particular, the antibody is used as a core reagent of various immunoassays, and plays an important role in the sensitivity and specificity of the immunoassay result. The preparation of high-specificity and high-titer antibodies is the basis of immunological techniques, but the antibodies of any preparation technique need to be separated and screened.
The existing antibody separating device can not rapidly separate and screen the antibody, and can not sufficiently separate and screen the antibody solution, so that the antibody separating and screening equipment is provided to solve the problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an antibody separation screening installation to reach above-mentioned purpose.
In order to achieve the above object, the utility model provides a following technical scheme:
an antibody separating and screening device comprises a base and a crushing and separating device, wherein the base is provided with a support, and the antibody separating and separating device is arranged on the support and comprises a first motor, a spiral stirring rod, an ultrasonic generator and a separating and crushing cavity, a liquid inlet is formed in the upper end surface of the separating and crushing cavity, the first motor is arranged on the upper end surface of the separating and crushing cavity, the spiral stirring rod penetrates through the upper end surface of the separating and crushing cavity in a rotating mode and is connected with the output end of the first motor, and the ultrasonic generator is arranged on the side wall of the separating and crushing cavity;
the lower end part of the crushing and separating device is provided with a centrifugal separating device for separating antibody solution, the upper end surface of a centrifugal separating cavity in the centrifugal separating device is provided with a centrifugal liquid inlet, the centrifugal liquid inlet is connected with the lower end part of the separating and crushing cavity, and the centrifugal liquid inlet is provided with a first electromagnetic valve;
the lower extreme of centrifugal separation device is provided with the deposit cavity of stewing, be provided with the precipitate export on the lower tip of the cavity of stewing of depositing, and deposit and be provided with the clear liquid export on the lateral wall of the cavity of stewing, still be provided with the screening box on the base, the clear liquid export is connected with the screening box, be provided with gel filtration screening layer and ion exchange screening layer in the inner chamber of screening box, still be provided with the liquid outlet on the lower extreme side of screening box.
In one alternative: the lower extreme setting of centrifugal separation device is on the base, still includes No. two motors and centrifugal separation bucket among the centrifugal separation device, the centrifugal separation cavity sets up on the base, No. two motor fixed mounting are on the inside wall of centrifugal separation cavity, the centrifugal separation bucket sets up and is provided with the plane bearing on the inside wall of centrifugal separation cavity and between centrifugal separation cavity lateral wall and the centrifugal separation bucket, be provided with the cyclic annular rack of round on the lateral wall of centrifugal separation bucket, be provided with the gear on the output of a motor, No. two motors are connected with the centrifugal separation bucket through the meshing of cyclic annular rack and gear, still be provided with centrifugal liquid outlet on the lower terminal surface of centrifugal separation bucket, centrifugal liquid outlet and deposit and stew and rotate between the cavity and be connected and be provided with No. two solenoid valve on the centrifugal liquid outlet.
In one alternative: a transparent window for observing the internal precipitation condition of the cavity is arranged on the side surface of the precipitation standing cavity, and a filtering film is arranged in the inner cavity of the separation crushing cavity.
In one alternative: still be provided with the water pump on the base, the one end of water pump is connected and is provided with the valve between water pump and clear liquid export through the pipe with the clear liquid export, and another tip of water pump is connected with the inner chamber of screening box through the pipe.
In one alternative: all be provided with sealed lid on inlet, the precipitate export and the liquid outlet, be provided with a plurality of fixed columns that are used for the separation and screening equipment to fix on the bottom face of base.
Compared with the prior art, the beneficial effects of the utility model are as follows:
the utility model discloses in be provided with broken separator and centrifugal separation device and carry out broken separation to antibody solution, compare in the better showing of its broken separation effect of the broken mode of traditional separation, simultaneously the utility model discloses in be provided with deposit the chamber of stewing can effectually divide the liquid to the antibody solution after the separation to can be to the better screening of antibody solution.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is an enlarged schematic view of a position a in fig. 1 of the present invention.
Fig. 3 is a schematic view of the centrifugal separation barrel of the present invention.
Notations for reference numerals:
wherein: the device comprises a support 1, a separation and crushing cavity 2, a liquid inlet 3, a first motor 4, a spiral stirring rod 5, a filtering film 6, an ultrasonic generator 7, a centrifugal liquid inlet 8, a first electromagnetic valve 9, a centrifugal separation cavity 10, a centrifugal separation barrel 11, an annular rack 12, a gear 13, a second motor 14, a plane bearing 15, a centrifugal liquid outlet 16, a second electromagnetic valve 17, a standing and precipitating cavity 18, a transparent window 19, a valve 20, a clear liquid outlet 21, a precipitate outlet 22, a water pump 23, a screening box body 24, a gel filtration screening layer 25, an ion exchange screening layer 27, a fixing column 28, a liquid outlet 29 and a base 30.
Detailed Description
The present invention will be described in detail with reference to the following embodiments, wherein like or similar elements are designated by like reference numerals throughout the drawings or description, and wherein the shape, thickness or height of the various elements may be expanded or reduced in practical applications. The embodiments of the present invention are provided only for illustration, and not for limiting the scope of the present invention. Any obvious and obvious modifications or alterations to the present invention can be made without departing from the spirit and scope of the present invention.
Example 1
Referring to fig. 1-3, in an embodiment of the present invention, an antibody separating and screening apparatus includes a base 30 and a crushing and separating device;
the base 30 is provided with a bracket 1, the crushing and separating device is arranged on the bracket 1 and comprises a first motor 4, a spiral stirring rod 5, an ultrasonic generator 7 and a separating and crushing cavity 2, a liquid inlet 3 is arranged on the upper end surface of the separation crushing cavity 2, the first motor 4 is arranged on the upper end surface of the separation crushing cavity 2, the spiral stirring rod 5 penetrates through the upper end surface of the separation crushing cavity 2 in a rotating way and is connected with the output end of the first motor 4, the ultrasonic generator 7 is arranged on the side wall of the separation crushing cavity 2, when the antibody solution is crushed and separated, the first motor 4 and the ultrasonic generator 7 are started, the ultrasonic generator 7 crushes the antibody solution in the inner cavity of the separation crushing cavity 2, meanwhile, the spiral stirring rod 5 is driven by the first motor 4 to rotate, so that the crushing and separating efficiency is further improved;
the lower end part of the crushing and separating device is provided with a centrifugal separating device for separating antibody solution, the upper end surface of a centrifugal separating cavity 10 in the centrifugal separating device is provided with a centrifugal liquid inlet 8, the centrifugal liquid inlet 8 is connected with the lower end part of the separating and crushing cavity 2, and the centrifugal liquid inlet 8 is provided with a first electromagnetic valve 9;
a sedimentation standing cavity 18 is arranged at the lower end of the centrifugal separation device, a sediment outlet 22 is arranged at the lower end of the sedimentation standing cavity 18, a clear liquid outlet 21 is arranged on the side wall of the sedimentation standing cavity 18, a screening box body 24 is also arranged on the base 30, the clear liquid outlet 21 is connected with the screening box body 24, a gel filtration screening layer 25 and an ion exchange screening layer 27 are arranged in the inner cavity of the screening box body 24, a liquid outlet 29 is also arranged on the side surface of the lower end of the screening box body 24, and the antibody solution after sedimentation is screened in the screening box body 24 sequentially through the gel filtration screening layer 25 and the ion exchange screening layer 27 and then is discharged from the liquid outlet 29 for collection;
the lower end of the centrifugal separation device is arranged on a base 30, the centrifugal separation device further comprises a second motor 14 and a centrifugal separation barrel 11, the centrifugal separation cavity 10 is arranged on the base 30, the second motor 14 is fixedly arranged on the inner side wall of the centrifugal separation cavity 10, the centrifugal separation barrel 11 is arranged on the inner side wall of the centrifugal separation cavity 10, a plane bearing 15 is arranged between the side wall of the centrifugal separation cavity 10 and the centrifugal separation barrel 11, a circle of annular rack 12 is arranged on the outer side wall of the centrifugal separation barrel 11, a gear 13 is arranged on the output end of the first motor 4, the second motor 14 is connected with the centrifugal separation barrel 11 through the meshing of the annular rack 12 and the gear 13, a centrifugal liquid outlet 16 is further arranged on the lower end face of the centrifugal separation barrel 11, the centrifugal liquid outlet 16 is rotatably connected with a sedimentation standing cavity 18, and a second electromagnetic valve 17 is arranged on the centrifugal liquid outlet 16, when the second motor 14 starts to work, the second motor 14 drives the centrifugal separation barrel 11 to start to rotate through the meshing of the annular rack 12 and the gear 13, the antibody solution in the centrifugal separation barrel 11 is centrifugally separated, the second electromagnetic valve 17 is opened after the antibody solution is separated, and the separated solution enters the precipitation standing cavity 18 for precipitation;
a transparent window 19 capable of observing the precipitation condition in the settling cavity is arranged on the side surface of the settling cavity 18, and a filter membrane 6 is arranged in the inner cavity of the separation crushing cavity 2;
the antibody solution screening device is characterized in that a water pump 23 is arranged above the base 30, one end of the water pump 23 is connected with the clear liquid outlet 21 through a guide pipe, a valve 20 is arranged between the water pump 23 and the clear liquid outlet 21, the other end of the water pump 23 is connected with the inner cavity of the screening box body 24 through a guide pipe, when the antibody solution in the sedimentation standing cavity 18 is stood for layering, sediment is discharged through a sediment outlet 22, and clear liquid after sedimentation is sent into the inner cavity of the screening box body 24 through the water pump 23 for screening.
Example 2
Referring to fig. 1, the embodiment of the present invention is different from embodiment 1 in that the liquid inlet 3, the precipitate outlet 22, and the liquid outlet 29 are all provided with a sealing cap for sealing, and the bottom end surface of the base 30 is provided with a plurality of fixing posts 28 for fixing the separation and screening device.
The utility model discloses a theory of operation is: fixing the whole separation and screening equipment on a proper position through a fixed column 28, injecting an antibody solution to be separated and screened into an inner cavity of a separation and crushing cavity 2 from a liquid inlet 3, starting a first motor 4 and an ultrasonic generator 7, crushing the antibody solution in the inner cavity of the separation and crushing cavity 2 by the ultrasonic generator 7, simultaneously rotating a spiral stirring rod 5 under the driving of the first motor 4 to further improve the crushing and separating efficiency, opening a first electromagnetic valve 9 after crushing and separating, filtering the antibody solution crushed by rough separation into a centrifugal separation cavity 10 through a filter membrane 6, then starting 14, driving a centrifugal separation barrel 11 to rotate by a second motor 14 through the meshing of an annular rack 12 and a gear 13, centrifugally separating the antibody solution in the centrifugal separation barrel 11, opening a second electromagnetic valve 17 after separating the antibody solution, the separated solution enters a precipitation standing cavity 18 for precipitation, the precipitation condition inside the precipitation standing cavity 18 can be observed through a transparent window 19, after the precipitation standing and layering, the precipitate is discharged through a precipitate outlet 22, the precipitated clear liquid is sent into an inner cavity of a screening box body 24 through a water pump 23 for screening, and the antibody solution is finally collected from a liquid outlet 29 through screening of a gel filtration screening layer 25 and an ion exchange screening layer 27.
The above description is only for the specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present disclosure, and all the changes or substitutions should be covered within the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.
Claims (5)
1. The antibody separating and screening equipment comprises a base (30) and a crushing and separating device, wherein a support (1) is arranged on the base (30), and the antibody separating and screening equipment is characterized in that the crushing and separating device is arranged on the support (1) and comprises a motor (4), a spiral stirring rod (5), an ultrasonic generator (7) and a separating and crushing cavity (2), a liquid inlet (3) is formed in the upper end face of the separating and crushing cavity (2), the motor (4) is arranged on the upper end face of the separating and crushing cavity (2), the spiral stirring rod (5) rotatably penetrates through the upper end face of the separating and crushing cavity (2) and is connected with the output end of the motor (4), and the ultrasonic generator (7) is arranged on the side wall of the separating and crushing cavity (2);
a centrifugal separation device for separating antibody solution is arranged at the lower end part of the crushing and separating device, a centrifugal liquid inlet (8) is arranged on the upper end surface of a centrifugal separation cavity (10) in the centrifugal separation device, the centrifugal liquid inlet (8) is connected with the lower end part of the separation crushing cavity (2), and a first electromagnetic valve (9) is arranged on the centrifugal liquid inlet (8);
the lower extreme of centrifugal separation device is provided with deposits cavity (18) that stews, it is provided with precipitate export (22) on the lower tip of cavity (18) to settle to stew, and is provided with clear liquid export (21) on the lateral wall of the cavity (18) that just settles to stew, still be provided with screening box (24) on base (30), clear liquid export (21) are connected with screening box (24), be provided with gel filtration screening layer (25) and ion exchange screening layer (27) in the inner chamber of screening box (24), still be provided with liquid outlet (29) on the lower extreme side of screening box (24).
2. The antibody separating and screening apparatus according to claim 1, wherein the lower end of the centrifugal separating device is disposed on a base (30), the centrifugal separating device further comprises a second motor (14) and a centrifugal separating barrel (11), the centrifugal separating cavity (10) is disposed on the base (30), the second motor (14) is fixedly mounted on the inner side wall of the centrifugal separating cavity (10), the centrifugal separating barrel (11) is disposed on the inner side wall of the centrifugal separating cavity (10), a plane bearing (15) is disposed between the side wall of the centrifugal separating cavity (10) and the centrifugal separating barrel (11), a ring-shaped rack (12) is disposed on the outer side wall of the centrifugal separating barrel (11), a gear (13) is disposed on the output end of the first motor (4), and the second motor (14) is connected with the centrifugal separating barrel (11) through the meshing of the ring-shaped rack (12) and the gear (13), still be provided with centrifugal liquid outlet (16) on the lower terminal surface of centrifugal separation bucket (11), centrifugal liquid outlet (16) and deposit and stew and rotate between the cavity (18) and be connected and be provided with No. two solenoid valve (17) on centrifugal liquid outlet (16).
3. The antibody separating and screening device according to claim 1, characterized in that a transparent window (19) for observing the deposition condition inside the settling chamber is arranged on the side surface of the settling chamber (18), and a filtering film (6) is arranged in the inner cavity of the separating and crushing chamber (2).
4. The antibody separating and screening device according to claim 2, wherein a water pump (23) is further arranged on the base (30), one end of the water pump (23) is connected with the clear liquid outlet (21) through a conduit, a valve (20) is arranged between the water pump (23) and the clear liquid outlet (21), and the other end of the water pump (23) is connected with the inner cavity of the screening box body (24) through a conduit.
5. The antibody separating and screening apparatus according to claim 1, wherein the liquid inlet (3), the precipitate outlet (22) and the liquid outlet (29) are provided with sealing caps, and the bottom end surface of the base (30) is provided with a plurality of fixing posts (28) for fixing the separating and screening apparatus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022404141.3U CN213967038U (en) | 2020-10-26 | 2020-10-26 | Antibody separation screening installation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022404141.3U CN213967038U (en) | 2020-10-26 | 2020-10-26 | Antibody separation screening installation |
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| Publication Number | Publication Date |
|---|---|
| CN213967038U true CN213967038U (en) | 2021-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022404141.3U Active CN213967038U (en) | 2020-10-26 | 2020-10-26 | Antibody separation screening installation |
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| CN (1) | CN213967038U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115055258A (en) * | 2022-06-15 | 2022-09-16 | 深圳仙草生物科技股份有限公司 | Adjustable ultrasonic extraction pharmaceutical equipment who smashes screening |
-
2020
- 2020-10-26 CN CN202022404141.3U patent/CN213967038U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115055258A (en) * | 2022-06-15 | 2022-09-16 | 深圳仙草生物科技股份有限公司 | Adjustable ultrasonic extraction pharmaceutical equipment who smashes screening |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
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| TR01 | Transfer of patent right |
Effective date of registration: 20240219 Address after: Room B1502, 15th Floor, Building 3, No. 88 Longyuan Road, Cangqian Street, Yuhang District, Hangzhou City, Zhejiang Province, 310000 Patentee after: Zhejiang Gaomei Gene Technology Co.,Ltd. Country or region after: China Address before: 1-2302-7, 15612 Century Avenue, high tech Zone, Jinan City, Shandong Province Patentee before: Shandong Saiya Biotechnology Co.,Ltd. Country or region before: China |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240308 Address after: Room 501, Building A2, No. 555 Chuangye Road, Dayun Town, Jiashan County, Jiaxing City, Zhejiang Province, 310000 (Residence application) Patentee after: Zhejiang Gaomei Biotechnology Co.,Ltd. Country or region after: China Address before: Room B1502, 15th Floor, Building 3, No. 88 Longyuan Road, Cangqian Street, Yuhang District, Hangzhou City, Zhejiang Province, 310000 Patentee before: Zhejiang Gaomei Gene Technology Co.,Ltd. Country or region before: China |
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| TR01 | Transfer of patent right |