CN206173353U - Improve device of quickly separating phosphoric acid albumen of structure - Google Patents
Improve device of quickly separating phosphoric acid albumen of structure Download PDFInfo
- Publication number
- CN206173353U CN206173353U CN201621062157.8U CN201621062157U CN206173353U CN 206173353 U CN206173353 U CN 206173353U CN 201621062157 U CN201621062157 U CN 201621062157U CN 206173353 U CN206173353 U CN 206173353U
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- China
- Prior art keywords
- housing
- protein
- phospoprotein
- phosphoric acid
- improved structure
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Abstract
The utility model discloses an improve device of quickly separating phosphoric acid albumen of structure, including casing and division board, the casing is divided into the cavity and descends the cavity through the division board, the top of casing be equipped with motor, reduction gear, protein add pipe and protein kinase add the pipe protein adds the pipe and the both ends that are located the reduction gear are respectively managed in the protein kinase interpolation, and protein adds the pipe and the protein kinase adds the inside that the one end of managing all extended to the casing. The practical structure is simple in structure, delicate in design, therefore, the carrier wave prepaid electric energy meter is low in cost, it separates in an organic whole to collect phosphoric acid protein preparation, through motor on the casing, pivot and stirring vane, can prepare phosphoric acid albumen in the casing, production dephosphorization acidizing phenomenon is placed for a long time to good phosphoric acid albumen to prevent the preparation, immediately through containing the separator tube to phosphoric acid protein discernment molecule particle after the preparation is good, the direct or indirect phosphoric acid albumen on the particle that will combine of filterable mode that utilizes that can be quick separates, rapid separation speed and little dosage.
Description
Technical field
This practicality is related to biotic experiment equipment technical field, more particularly to a kind of sharp separation Phospoprotein of improved structure
Device.
Background technology
Important function of the phosphorylated protein in cell activities so as to become the focus of proteomics research.But
It is the Some features of phosphorylated protein itself so that phosphorylating protein analysis is still one and has very much challenging work, first,
Phosphorylating protein content is very low, only has small part albumen phosphorylation occurs under particular stimulation, secondly, phosphorylation it is variable
Property so that under different condition there are different phosphorylation forms in albumen, and finally, the presence of phosphatase is caused in Sample Preparation Procedure
Produce dephosphorylation phenomenon.Therefore phosphorylated protein or phosphated peptide section are enriched with, improve the relative of phosphoeptide and contain
Amount, becomes the important content in the research of phosphorylated protein group.
The separation analysis of Phospoprotein is realized frequently with the antibody of Phospoprotein, using Phospoprotein and the knot of corresponding antibodies
Cooperation is used, and albumen is separated from the Cell sap of cracking the analysis for carrying out next step, but antibody and Phospoprotein are molten
Yu Shui, how the antibody with reference to Phospoprotein is extracted from separating liquid is a more complicated technique, and phosphoric acid egg at present
Bai Kangti exists expensive, and stability is poor, the low problem of the protein concentration after extraction, when separating to Phospoprotein,
If using not being the phosphorylated protein that just produced, prolonged storage can make phosphorylated protein produce dephosphorylation phenomenon,
The preparation rate of Phospoprotein is lower, is this it is proposed that a kind of device of the sharp separation Phospoprotein of improved structure, solves
The problems referred to above.
Utility model content
This practical purpose is that, in order to solve shortcoming present in prior art, and a kind of improved structure for proposing is quick
Separate the device of Phospoprotein.
To achieve these goals, this practicality employs following technical scheme：
A kind of device of the sharp separation Phospoprotein of improved structure, including housing and demarcation strip, housing pass through demarcation strip
It is divided into upper chamber and lower chambers, the top of the housing is provided with the addition of motor, decelerator, protein adding tube and protein kinase
Pipe, the protein adding tube and protein kinase adding tube are located at the two ends of decelerator, and protein adding tube and albumen respectively
One end of matter kinases adding tube all extends to the inside of housing, and the output shaft of motor is connected with the input shaft of decelerator, slows down
The output shaft of device is connected with rotating shaft, and rotating shaft is located in the epicoele room of housing, and the side wall of rotating shaft is provided with stirring vane, the separation
There can be first through hole on plate, in first through hole, be provided with conduit, conduit is located in lower chambers, and conduit is provided with the first control valve, leads
The bottom of pipe is connected with separating pipe, and the inside of separating pipe is provided with the multiple drainage screens and filter membrane be arrangeding in parallel, and filter membrane is located at and filters
The lower section of net, drainage screen are provided with the microgranule of absorption phosphorylated protein, and the bottom of separating pipe is connected with filtrate box, filtrate box activity
Installed in the inner bottom of housing, the side wall bottom of filtrate box is connected with discharge nozzle, and discharge nozzle extends away from one end of filtrate box
To the outside of housing.
Preferably, the microgranule is metal-oxide or metal hydroxidess, the metal-oxide or metal hydroxide
Thing is TiO2, Al2O3, ZrO2, Fe2O3 or Al (OH) 3.
Preferably, the rotating shaft is provided with six to eight stirring vanes, and stirring vane is provided with the second through hole.
Preferably, the side side wall of the housing is provided with opening, and portable plate is provided with opening, and portable plate is located at lower chambers
In, portable plate is provided with hinge with the junction of housing.
Preferably, the top of the housing is provided with third through-hole, and the output shaft of decelerator is connected with through third through-hole and turns
Axle, third through-hole are provided with bearing and sealing ring with the output between centers of decelerator, and bearing is located at the top of sealing ring.
Preferably, the discharge nozzle is provided with the second control valve away from one end of housing.
Preferably, the bottom of the housing is provided with runner, and runner is provided with fixing device.
Preferably, the housing and demarcation strip are integral type structure.
This practical beneficial effect：Simple structure, designs ingenious, with low cost, integrates Phospoprotein preparative separation,
By in housing upper motor, rotating shaft and stirring vane, Phospoprotein being prepared in housing, prevent the Phospoprotein for preparing
Long-time is placed and produces dephosphorylation phenomenon, immediately by recognizing molecule microgranule containing for phosphoprotein after preparing
Separating pipe, quickly can be separated using the direct or indirect Phospoprotein being incorporated on microgranule of the mode for filtering, separate speed
Degree is fast, while multiple drainage screens are provided with separating pipe, filter effect is more preferable.
Description of the drawings
Fig. 1 is a kind of structural representation of the device of the sharp separation Phospoprotein of improved structure that this practicality is proposed.
In figure：1 housing, 2 filtrate box, 3 filter membranes, 4 separating pipes, 5 drainage screens, 6 conduits, 7 first control valves, 8 protein add
Plus pipe, 9 motor, 10 decelerators, 11 protein kinase adding tubes, 12 rotating shafts, 13 stirring vanes, 14 discharge nozzles.
Specific embodiment
Below in conjunction with the accompanying drawing in this practical embodiment, the technical scheme in this practical embodiment is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only this practical a part of embodiment, rather than the embodiment of whole.
With reference to Fig. 1, a kind of device of the sharp separation Phospoprotein of improved structure, including housing 1 and demarcation strip, housing 1
Upper chamber and lower chambers are divided into by demarcation strip, the top of housing 1 is provided with motor 9, decelerator 10,8 and of protein adding tube
Protein kinase adding tube 11, protein adding tube 8 and protein kinase adding tube 11 are located at the two ends of decelerator 10, and albumen respectively
One end of matter adding tube 8 and protein kinase adding tube 11 all extends to the inside of housing 1, output shaft and the deceleration of motor 9
The input shaft connection of device 10, the output shaft of decelerator 10 are connected with rotating shaft 12, and rotating shaft 12 is located in the epicoele room of housing 1, rotating shaft
12 side wall is provided with stirring vane 13, can have first through hole on demarcation strip, and conduit 6 is provided with first through hole, and conduit 6 is located at down
In chamber, conduit 6 is provided with the first control valve 7, and the bottom of conduit 6 is connected with separating pipe 4, and the inside of separating pipe 4 is provided with parallel
The multiple drainage screens 5 for arranging and filter membrane 3, filter membrane 3 are located at the lower section of drainage screen 5, and drainage screen 5 is provided with absorption phosphorylated protein
Microgranule, the bottom of separating pipe 4 are connected with filtrate box 2, and filtrate box 2 is movably arranged on the inner bottom of housing 1, the side of filtrate box 2
Wall bottom is connected with discharge nozzle 14, and discharge nozzle 14 extends to the outside of housing 1 away from one end of filtrate box 2, and microgranule is metal oxygen
Compound or metal hydroxidess, the metal-oxide or metal hydroxidess are TiO2, Al2O3, ZrO2, Fe2O3 or Al
(OH) 3, rotating shaft 12 is provided with six to eight stirring vanes 13, and stirring vane 13 is provided with the second through hole, the side side of housing 1
Wall is provided with opening, and portable plate is provided with opening, and portable plate is located in lower chambers, and portable plate is provided with hinge with the junction of housing
Chain, the top of housing 1 are provided with third through-hole, and the output shaft of decelerator 10 is connected with rotating shaft 12, third through-hole through third through-hole
Bearing and sealing ring are provided with the output between centers of decelerator 10, bearing is located at the top of sealing ring, and discharge nozzle 14 is away from housing 1
One end is provided with the second control valve, and the bottom of housing 1 is provided with runner, and runner is provided with fixing device, and housing 1 and demarcation strip are integrated
Formula structure.
Operation principle：Protein solution and protein kinase are passed through into protein adding tube 8 respectively and protein kinase adds
During pipe 11 adds the upper chamber of housing 1, drive rotating shaft 12 to rotate by motor 9, the rotating speed of rotating shaft 12 controlled by decelerator,
Stirring vane 13 is sufficiently stirred for solution, protein solution is fully reacted with protein kinase, and the Phospoprotein for producing impurity is molten
Liquid, the Phospoprotein solution of impurity are entered in separating pipe 4 by conduit 6, phosphorylated protein therein as identification molecule with
Metal-oxide or metal hydroxidess identification on drainage screen 5 is combined, and then washes impurity off with buffer, by filtrate box 2
Collect and change filtrate, then Phospoprotein pure on lower identification molecule is washed by eluent, collect or be used for subsequent analysis.
The above, is only this practicality preferably specific embodiment, but this practical protection domain is not limited thereto,
Any those familiar with the art in the technical scope that this practicality is disclosed, according to this practical technical scheme and its
Practical design in addition equivalent or change, should all cover within this practical protection domain.
Claims (8)
1. a kind of device of the sharp separation Phospoprotein of improved structure, including housing（1）And demarcation strip, housing（1）By dividing
Dividing plate is divided into upper chamber and lower chambers, it is characterised in that the housing（1）Top be provided with motor（9）, decelerator（10）、
Protein adding tube（8）With protein kinase adding tube（11）, the protein adding tube（8）With protein kinase adding tube（11）Point
Wei Yu not decelerator（10）Two ends, and protein adding tube（8）With protein kinase adding tube（11）One end all extend to
Housing（1）Inside, motor（9）Output shaft and decelerator（10）Input shaft connection, decelerator（10）Output shaft connect
It is connected to rotating shaft（12）, rotating shaft（12）Positioned at housing（1）Epicoele room in, rotating shaft（12）Side wall be provided with stirring vane（13）,
There can be first through hole on the demarcation strip, in first through hole, be provided with conduit（6）, conduit（6）In lower chambers, conduit（6）On
It is provided with the first control valve（7）, conduit（6）Bottom be connected with separating pipe（4）, separating pipe（4）Inside be provided with what is be arranged in parallel
Multiple drainage screens（5）And filter membrane（3）, filter membrane（3）Positioned at drainage screen（5）Lower section, drainage screen（5）It is provided with absorption phosphorylation egg
White microgranule, separating pipe（4）Bottom be connected with filtrate box（2）, filtrate box（2）It is movably arranged on housing（1）Inner bottom,
Filtrate box（2）Side wall bottom be connected with discharge nozzle（14）, discharge nozzle（14）Away from filtrate box（2）One end extend to housing
（1）Outside.
2. the device of the sharp separation Phospoprotein of a kind of improved structure according to claim 1, it is characterised in that described
Microgranule is metal-oxide or metal hydroxidess, the metal-oxide or metal hydroxidess be TiO2, Al2O3, ZrO2,
Fe2O3 or Al (OH) 3.
3. the device of the sharp separation Phospoprotein of a kind of improved structure according to claim 1, it is characterised in that described
Rotating shaft（12）It is provided with six to eight stirring vanes（13）, and stirring vane（13）It is provided with the second through hole.
4. the device of the sharp separation Phospoprotein of a kind of improved structure according to claim 1, it is characterised in that described
Housing（1）Side side wall be provided with opening, be provided with portable plate at opening, portable plate is located in lower chambers, portable plate and housing
Junction be provided with hinge.
5. the device of the sharp separation Phospoprotein of a kind of improved structure according to claim 1, it is characterised in that described
Housing（1）Top be provided with third through-hole, decelerator（10）Output shaft be connected with rotating shaft through third through-hole（12）, threeway
Hole and decelerator（10）Output between centers be provided with bearing and sealing ring, bearing is located at the top of sealing ring.
6. the device of the sharp separation Phospoprotein of a kind of improved structure according to claim 1, it is characterised in that described
Discharge nozzle（14）Away from housing（1）One end be provided with the second control valve.
7. the device of the sharp separation Phospoprotein of a kind of improved structure according to claim 1, it is characterised in that described
Housing（1）Bottom be provided with runner, runner is provided with fixing device.
8. the device of the sharp separation Phospoprotein of a kind of improved structure according to claim 1, it is characterised in that described
Housing（1）It is integral type structure with demarcation strip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621062157.8U CN206173353U (en) | 2016-09-19 | 2016-09-19 | Improve device of quickly separating phosphoric acid albumen of structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621062157.8U CN206173353U (en) | 2016-09-19 | 2016-09-19 | Improve device of quickly separating phosphoric acid albumen of structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206173353U true CN206173353U (en) | 2017-05-17 |
Family
ID=58676238
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621062157.8U Expired - Fee Related CN206173353U (en) | 2016-09-19 | 2016-09-19 | Improve device of quickly separating phosphoric acid albumen of structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206173353U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107474104A (en) * | 2017-09-21 | 2017-12-15 | 浙江绿创生物科技有限公司 | A kind of multistage Protein Separation collecting device of improved structure |
| CN109011821A (en) * | 2018-09-18 | 2018-12-18 | 安徽源森生物科技有限公司 | It is a kind of for manufacturing the filter of rice protein |
-
2016
- 2016-09-19 CN CN201621062157.8U patent/CN206173353U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107474104A (en) * | 2017-09-21 | 2017-12-15 | 浙江绿创生物科技有限公司 | A kind of multistage Protein Separation collecting device of improved structure |
| CN109011821A (en) * | 2018-09-18 | 2018-12-18 | 安徽源森生物科技有限公司 | It is a kind of for manufacturing the filter of rice protein |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170517 Termination date: 20170919 |
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| CF01 | Termination of patent right due to non-payment of annual fee |