CN213933890U - Sample analyzer - Google Patents

Abstract

A sample analyzer comprises a blending position and an ultrasonic device, wherein the ultrasonic device comprises an ultrasonic transducer, a transmission piece and a moving device, the ultrasonic transducer is used for forming ultrasonic vibration, the transmission piece is provided with a first end and a second end, the first end of the transmission piece is connected with the ultrasonic transducer, and the outer diameter of the second end of the transmission piece is smaller than the inner diameter of an accommodating cup; the moving device is connected with the ultrasonic transducer, the moving device is used for driving the ultrasonic transducer and the transmission piece to move relative to the containing cup on the blending position, and the second end of the transmission piece can be inserted into liquid in the containing cup so as to transmit ultrasonic vibration generated by the ultrasonic transducer to the liquid in the containing cup. Due to the arrangement of the ultrasonic device, the ultrasonic device can emit ultrasonic waves to the reaction liquid to uniformly mix the reaction liquid, ultrasonic mixing is realized by adopting ultrasonic vibration and the effect of forming ultrasonic cavitation, and compared with traditional mechanical mixing, the ultrasonic mixing has the advantages of sufficient mixing, reaction liquid splashing prevention and the like.

Description

Sample analyzer

Technical Field

The utility model relates to an external check out test set, concretely relates to sample analyzer.

Background

In vitro diagnosis refers to products and services for determining diseases or body functions by detecting human body samples, such as blood, urine, etc., to obtain clinical diagnosis information. Since in vitro diagnostic modalities allow rapid and accurate diagnosis in the early stages of disease, they play an increasingly important role in the field of clinical medicine and related medical research.

In the process of detecting a human body sample, generally, a sample to be detected and a corresponding reagent are mixed to form a reaction solution, and then a mixing device is used for mixing the reaction solution, so that the sample and the reagent can react fully. Traditional mixing device adopts modes such as stirring, vibration more, and this leads to appearing the reaction liquid spill easily in the mixing process, phenomenon such as mixing effect is not good to influence the accuracy of final testing result.

Disclosure of Invention

In one embodiment there is provided a sample analyzer comprising:

the blending position is used for placing the containing cup;

the ultrasonic device comprises an ultrasonic transducer, a transmission piece and a moving device, wherein the ultrasonic transducer is used for generating ultrasonic vibration, the transmission piece is provided with a first end and a second end, the first end of the transmission piece is connected with the ultrasonic transducer, and the outer diameter of the second end of the transmission piece is smaller than the inner diameter of the accommodating cup; the moving device is connected with the ultrasonic transducer, the moving device is used for driving the ultrasonic transducer and the transmission piece to move relative to the containing cup on the blending position, and the second end of the transmission piece can be inserted into liquid in the containing cup so as to transmit ultrasonic vibration generated by the ultrasonic transducer to the liquid in the containing cup.

In one embodiment, the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or is reduced in a stepped manner from the first end to the second end.

In one embodiment, the first end of the transmission member is in threaded connection or clamped connection with the ultrasonic transducer, and the axial end face of the second end of the transmission member is used for transmitting ultrasonic waves.

In one embodiment, the moving device comprises a mounting seat, a swing arm assembly and a first moving assembly, the swing arm assembly and the first moving assembly are mounted on the mounting seat, the ultrasonic transducer is mounted on the swing arm assembly, the first moving assembly is connected with the swing arm assembly, and the first moving assembly is used for driving the transmission piece to be inserted into or removed from the accommodating part.

In one embodiment, the moving device further includes a second moving assembly, the second moving assembly is mounted on the mounting base, the second moving assembly is connected with the swing arm assembly, and the second moving assembly is used for driving the ultrasonic transducer and the transmission member to move in the horizontal direction.

In one embodiment, the sample analyzer further includes a sample carrying mechanism, a sample dispensing mechanism, a reagent carrying mechanism, a reagent dispensing mechanism, a reaction mechanism, and a measurement mechanism, wherein the sample carrying mechanism is configured to carry a sample, the sample dispensing mechanism is configured to suck the sample and discharge the sample into the receiving cup, the reagent carrying mechanism is configured to carry a reagent, the reagent dispensing mechanism is configured to suck the reagent and discharge the reagent into the receiving cup, the ultrasonic device is configured to perform a blending operation on a reaction solution formed by mixing the reagent and the sample in the receiving cup, the reaction mechanism is configured to provide an incubation place for the reaction solution in the receiving cup, and the measurement mechanism is configured to measure the reaction solution.

In one embodiment there is provided a sample analyzer comprising:

the blending position is used for placing the containing cup;

and an ultrasonic device comprising an ultrasonic transducer and a transmission member, the ultrasonic transducer being configured to generate ultrasonic vibrations, the transmission member having a first end and a second end, the first end of the transmission member being connected to the ultrasonic transducer; the second end of the transmission piece is used for abutting against the outer wall of the containing cup positioned on the blending position, and the part of the outer wall of the containing cup, which is in contact with the transmission piece, is a part surrounding the reaction liquid so as to transmit the ultrasonic vibration generated by the ultrasonic transducer into the liquid in the containing cup.

In one embodiment, the sample analyzer further comprises a clasping means for clasping the containment cup.

In one embodiment, the clamping device comprises two clamping components which are arranged relatively, each clamping component comprises a clamping motor, a clamping cam and a clamping block, the clamping motor is connected with the clamping cam in a transmission mode, the clamping cam is connected with the clamping block in a contact mode, the clamping motor is used for driving the clamping cam to rotate, and therefore the clamping cam drives the clamping block to be close to or far away from the containing cup.

In one embodiment, the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or is reduced in a stepped manner from the first end to the second end.

In one embodiment, an axial end face of the second end of the transmission member is used for transmitting ultrasonic waves.

In one embodiment, the ultrasonic device further comprises a moving device, the moving device is connected with the ultrasonic transducer, and the moving device is used for driving the second end of the transmission member to abut against or leave the containing cup located on the blending position.

In one embodiment, the sample analyzer further includes a sample carrying mechanism, a sample dispensing mechanism, a reagent carrying mechanism, a reagent dispensing mechanism, a reaction mechanism, and a measurement mechanism, wherein the sample carrying mechanism is configured to carry a sample, the sample dispensing mechanism is configured to suck the sample and discharge the sample into the receiving cup, the reagent carrying mechanism is configured to carry a reagent, the reagent dispensing mechanism is configured to suck the reagent and discharge the reagent into the receiving cup, the ultrasonic device is configured to perform a blending operation on a reaction solution formed by mixing the reagent and the sample in the receiving cup, the reaction mechanism is configured to provide an incubation place for the reaction solution in the receiving cup, and the measurement mechanism is configured to measure the reaction solution.

According to the sample analyzer of the embodiment, due to the fact that the ultrasonic device is arranged, the ultrasonic device can carry out ultrasonic mixing on liquid in the containing cup, the ultrasonic device can emit ultrasonic waves to the reaction liquid to mix the liquid uniformly, the ultrasonic mixing is achieved by adopting ultrasonic vibration and the effect of ultrasonic cavitation, compared with traditional mechanical mixing, the ultrasonic mixing has the advantages of being sufficient in mixing, capable of avoiding reaction liquid from splashing and the like.

Drawings

FIG. 1 is a schematic diagram of an embodiment of an apparatus for analyzing an immune luminescence;

FIG. 2 is a block diagram showing the structure of a control section of an immunofluorescence analyzer in an embodiment;

FIG. 3 is a schematic diagram of a contact ultrasonic apparatus according to an embodiment;

FIG. 4 is a structural view of a transmission member in one embodiment;

FIG. 5 is a structural view of a transmission member in one embodiment;

FIG. 6 is a diagram illustrating a mobile device according to an embodiment;

FIG. 7 is a schematic illustration of ultrasonic blending in one embodiment;

FIG. 8 is a schematic diagram of a non-contact ultrasonic apparatus according to an embodiment;

FIG. 9 is a schematic diagram of a non-contact ultrasonic apparatus according to an embodiment;

FIG. 10 is a side view of a clasping means in an embodiment;

FIG. 11 is a top view of a clasping means in one embodiment;

FIG. 12 is a timing diagram of a sample analysis method in one embodiment;

FIG. 13 is a flow diagram of a method for sample analysis in one embodiment.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The testing process of the sample analyzer comprises the steps of loading, adding reagent, incubating, magnetically separating, adding substrate and the like, wherein each step is either added with a substance or needs to transfer a sample, so that each step has the requirement of uniform mixing. The existing mechanical motion modes such as vortex, shaking and the like are used for uniformly mixing the sample or the reaction liquid, namely, uniform mixing is realized through the vortex of the liquid and the collision between the liquid and the containing cup, the mechanical uniform mixing cannot achieve the effect of aggregating tighter substances in the liquid, and the liquid is easily spilled out to cause detection data errors. Furthermore, mechanical type mixing device need with place the cup stand that holds the cup on the mixing position and be connected, a mechanical type mixing device can't carry out the mixing to holding the cup on two mixing positions, consequently to the sample analysis appearance that needs set up a plurality of mixing positions, a mechanical mixing device need be installed to every mixing position, the structure is complicated relatively, occupation space is also big.

This application adopts the mode of supersound mixing to carry out the mixing to the well liquid that holds the cup, and transducer ultrasonic vibration among the ultrasonic device, ultrasonic vibration transmits and forms the ultrasonic field in the liquid in holding the cup, and ultrasonic vibration will drive liquid flow and realize the mixing to reach a definite value after, will produce the ultrasonic cavitation phenomenon in the liquid, the ultrasonic cavitation phenomenon forms the bubble blasting in the liquid, can disperse the inseparabler material of reunion in the liquid, realizes evenly dispersed liquid, and the ultrasonic mixing is also difficult for spilling liquid. Moreover, the ultrasonic device is directly inserted into contact ultrasonic mixing of liquid and non-contact ultrasonic mixing of ultrasonic vibration transmitted through the outer wall of the containing cup, the ultrasonic vibration can be transmitted into the liquid of the containing cup, the ultrasonic device is not required to be connected with the cup seat on the mixing position, and therefore the ultrasonic mixing of the liquid in the containing cup on a plurality of mixing positions is carried out by one movable ultrasonic device, the structure is simple, and the occupied space is small.

In one embodiment, an ultrasonic device is arranged in the sample analyzer, and the reaction liquid is ultrasonically mixed through the ultrasonic device, so that the detection accuracy of the reaction liquid is improved. The sample analyzer may be a biochemical analyzer or an immunoassay analyzer, and the present embodiment takes an immunoassay analyzer as an example for explanation.

Referring to fig. 1 and 2, the immunofluorescence analyzer mainly includes an ultrasonic device 10, a sample holding mechanism 21, a sample dispensing mechanism 22, a reagent holding mechanism 31, a reagent dispensing mechanism 32, a reaction mechanism 40, a magnetic separation mechanism 50, and a controller 60. The sample support mechanism 21, the sample dispensing mechanism 22, the reagent support mechanism 31, the reagent dispensing mechanism 32, the reaction mechanism 40, the magnetic separation mechanism 50, and the kneading apparatus are all mounted on the base 100, the controller 60 is mounted on the main body of the base 100, and the controller 60 may also be mounted on the base 100. The biochemical analyzer does not include the magnetic separation mechanism described above.

The immune luminescence analyzer further comprises a cup loading mechanism 71, a cup throwing position 72, a first transfer mechanism 81, a second transfer mechanism 82 and a measuring mechanism 90 which are arranged on the base 100.

Wherein, the reaction mechanism 40 is arranged in the middle, and the reagent bearing mechanism 31, the magnetic separation mechanism 50, the ultrasonic device 10, the cup feeding mechanism 71, the cup throwing position 72 and the measuring mechanism 90 are respectively arranged around the reaction mechanism 40.

The cup loading mechanism 71 is used for storing a new used receiving cup 103, and the receiving cup 103 is also called a reaction cup. The cup loading mechanism 71 itself also has a cup moving function, and can move the storage cup 103 from the storage position to a position to be gripped.

The first transfer mechanism 81 is disposed between the upper cup mechanism 71 and the reaction mechanism 40, the first transfer mechanism 81 is a cup grasping mechanism, and the first transfer mechanism 81 is used for transferring a new receiving cup 103 on the upper cup mechanism 71 to the sample application site 101 near the reaction mechanism 40 and transferring the receiving cup 103 on the sample application site 101 to the reaction mechanism 40.

The cup throwing position 72 is located in the moving range of the first transfer mechanism 81, the cup throwing position 72 is connected with the recycling box, and the cup throwing position 72 is used for recycling the used accommodating cup 103. The first transfer mechanism 81 is also used for transferring the detected containing cup 103 on the reaction mechanism 40 to the cup throwing position 72.

The sample support mechanism 21 is used for supporting a sample. The Sample support mechanism 21 may include, in some examples, a Sample Delivery Module (SDM); in other examples, the sample carrier mechanism 21 may be a sample tray including a plurality of sample sites for placing samples, such as sample tubes, and the sample tray may be configured to rotate to dispatch the samples to corresponding locations, such as locations for the sample dispensing mechanism 22 to aspirate the samples.

The sample dispensing mechanism 22 includes a sampling needle, a moving mechanism for driving the sampling needle to move in two or three dimensions between the sample loading mechanism 21 and the sample addition site 101, and a drive pump for providing a sample sucking and discharging power to the sampling needle. The sample dispensing mechanism 22 is used for sucking the sample in the sample tube on the sample carrying mechanism 21 and for dispensing the sucked sample into the receiving cup 103 on the dispensing position 101.

The reagent carrying mechanism 31 is for carrying a reagent. In one embodiment, the reagent carrying mechanism 31 may be a reagent disk, the reagent disk is configured in a disk-shaped structure and has a plurality of positions for carrying reagent containers, and the reagent carrying mechanism 31 can rotate and drive the reagent containers carried by the reagent carrying mechanism 31 to rotate to a specific position, for example, a position for sucking reagent by the reagent dispensing mechanism 32. The number of the reagent carrying means 31 may be one or more.

The reagent dispensing mechanism 32 includes a reagent needle, a moving mechanism for driving the reagent needle to move two-dimensionally or three-dimensionally between the reagent holding mechanism 31 and the reaction mechanism 40, and a drive pump for supplying power to the reagent needle for sucking and discharging a reagent. The reagent dispensing mechanism 32 is used for sucking the reagent in the reagent tube on the reagent bearing mechanism 31 and for filling the sucked reagent into the containing cup 103 provided with the sample on the reaction mechanism 40, and the sample and the reagent in the containing cup 103 are mixed and reacted to form a reaction liquid.

The reaction mechanism 40 is used for providing an incubation place for the reaction solution, the reaction mechanism 40 can be a reaction disc which is arranged in a disc-shaped structure and provided with one or more placing positions for placing reaction cups, and the reaction disc can rotate and drive the reaction cups in the placing positions to rotate and is used for scheduling the reaction cups in the reaction disc and incubating the reaction solution in the reaction cups.

The magnetic separation mechanism 50 comprises a cleaning solution dispensing structure, a magnetic absorption structure, a liquid absorption structure and a substrate dispensing structure, wherein the cleaning solution dispensing structure is used for dispensing a cleaning solution into the incubated reaction solution, and the cleaning solution is used for separating free substances in the incubated reaction solution; the magnetic attraction structure is used for performing magnetic attraction operation on the reaction liquid filled with the cleaning liquid, and is used for adsorbing a reaction compound combined with the magnetic beads; the liquid-absorbing structure is used for discharging the components other than the reaction complex bound to the magnetic beads out of the receiving cup 103; the substrate dispensing mechanism is used to dispense a substrate into the reaction solution in the receiving cup 103, and the substrate reacts with the reaction complex in the reaction solution, and the substrate labels the reaction complex by light emission.

The magnetic separation mechanisms 50 are provided in two, and the two magnetic separation mechanisms 50 can work independently of each other to improve the efficiency of the test.

A mixing position 102 is provided at a position close to the reaction mechanism 40 and the magnetic separation mechanism 50, a cup holder for placing the holding cup 103 is provided at the mixing position 102 and the sample addition position 101, and the second transfer mechanism 82 is used for transferring the holding cup 103 among the reaction mechanism 40, the magnetic separation mechanism 50 and the mixing position 102.

The measurement means 90 is used to perform optical measurement on the reaction solution after completion of incubation, and obtain reaction data of the sample. For example, the measuring unit 90 detects the light emission intensity of the reaction solution to be measured, and calculates the concentration of the component to be measured in the sample from the calibration curve.

The base 100 is further provided with a cleaning mechanism and a waste liquid suction mechanism, the cleaning mechanism is used for cleaning the sampling needle and the reagent needle, and the waste liquid suction mechanism is used for sucking reaction liquid after detection.

Wherein, the sample adding position 101 and the mixing position 102 are both provided with cup holders, the cup holders are provided with placing grooves for placing the holding cups 103, and the cup holders are installed on the machine base 100 or the cup holders and the machine base 100 are of an integrated structure.

In this embodiment, the ultrasonic device 10 is disposed near the mixing position 102, and the ultrasonic device 10 can emit ultrasonic waves into a reaction liquid in the containing cup 103 on the mixing position 102, where the reaction liquid may be a reaction liquid before or after incubation, and the reaction liquid may also be a reaction liquid before or after magnetic attraction separation.

In one embodiment, the ultrasonic device 10 is disposed near the sample application site 101, so that the ultrasonic device 10 can perform ultrasonic mixing on the sample in the containing cup 103 on the sample application site 101.

In one embodiment, the ultrasound device 10 is disposed proximate to the reagent support 31 such that the ultrasound device 10 is capable of ultrasonically mixing the reagent within the reagent support 31.

In one embodiment, at least two mixing positions 102 are provided, the placing positions of the containing cups 103 are increased, one ultrasonic device 10 corresponds to at least two mixing positions 102, that is, one ultrasonic device 10 can perform ultrasonic mixing on reaction liquids 104 in a plurality of containing cups 103 respectively, one containing cup 103 can perform transfer or other operations during ultrasonic mixing, the containing cups 103 on other mixing positions 102 can perform transfer or other operations, and the containing cups 103 on a plurality of mixing positions 102 realize alternate mixing, so as to improve the detection efficiency.

Referring to fig. 3, in the present embodiment, the ultrasonic apparatus 10 is a device independent from other mechanisms, that is, the ultrasonic apparatus 10 can operate independently, for example, the ultrasonic apparatus 10 can operate independently from the sample dispensing mechanism 22, and the ultrasonic apparatus 10 can operate synchronously or asynchronously with other mechanisms, so as to improve the efficiency of item detection.

The ultrasonic device 10 of this embodiment is a contact ultrasonic device, and ultrasonic device 10 includes ultrasonic transducer 11, transmission piece 12 and mobile device 13, and ultrasonic transducer 11 is including the backing layer, piezoelectric layer and the matching layer that meet in proper order, and the piezoelectric layer is piezoelectric crystal, and piezoelectric crystal produces the compression and the inflation of thickness direction through the inverse piezoelectric effect under the effect of driving electrical signal, and the frequency of this kind of deformation reaches ultrasonic frequency, forms ultrasonic vibration. The ultrasonic transducer 11 is connected with the controller 60, and the controller 60 is used for controlling the output power and the output duration of the ultrasonic transducer 11 so as to realize a plurality of ultrasonic blending modes with different ultrasonic intensities and different ultrasonic durations.

Referring to fig. 3 and 4, the transmission member 12 is a solid rod-shaped structure, the transmission member 12 has a first end and a second end, the first end is an upper end, the second end is a lower end, the first end of the transmission member 12 is provided with an external thread, the lower end of the ultrasonic transducer 11 is provided with an internal thread, the transmission member 12 is installed at the lower end of the ultrasonic transducer 11 by a threaded connection manner, and the transmission member 12 can also be connected with the ultrasonic transducer 11 by other manners such as clamping. The transmission member 12 is a resonance rod, the transmission member 12 is connected to the matching layer of the ultrasonic transducer 11, and the transmission member 12 transmits the ultrasonic vibration. Compared with the transmission member 12 with a hollow structure, the solid transmission member 12 is beneficial to the transmission of axial vibration, and when the outer diameter of the transmission member 12 is reduced along the transmission direction of ultrasonic vibration, the solid transmission member 12 is beneficial to the convergence of energy so as to realize better ultrasonic uniform mixing effect.

The external diameter of the transmission part 12 is gradually reduced or reduced in a step mode from the first end to the second end, the transmission part 12 has the function of energy gathering, when the ultrasonic vibration is transmitted from the first end to the second end, the axial cross-sectional area of the second end relative to the first end is reduced, the ultrasonic vibration is gathered more at the second end relative to the first end, the amplitude of the emergent ultrasonic vibration is enlarged by the second end of the transmission part 12 relative to the first end, and therefore the emergent ultrasonic energy is improved.

Specifically, the transmission member 12 includes a first end 121, an intermediate section 122, and a second end 123, wherein the first end 121 is an externally threaded connecting end, the second end 123 is a needle bar structure, and the second end 123 has an outer diameter smaller than the inner diameter of the receiving cup 103, such that the second end 123 of the transmission member 12 can be inserted into the receiving cup 103. Middle section 122 is loudspeaker column structure, and the one end that middle section 122 and first end 121 are connected is the loudspeaker main aspects, and the one end that middle section 122 and second end 123 are connected is the loudspeaker tip, and the diameter of axle that middle section 122 followed the loudspeaker main aspects to the loudspeaker tip reduces gradually.

The intermediate section 122 may also be comprised of one or any combination of cylindrical and conical rods. Referring to FIG. 5, the middle section 122 of the a-structure includes two cylindrical rods with different diameters; the middle section 122 of the b-structure comprises four cylindrical rods with different diameters; the middle section 122 of the c-configuration comprises a section of conical rod; the intermediate section 122 of the d-configuration comprises two cylindrical rods of different diameters and one conical rod. The five structures of the transmission member 12 are all structures that gradually decrease or step-wise decrease from the first end to the second end, and can play a role in amplifying the amplitude.

Referring to fig. 6, the moving device 13 includes a mounting seat 131, a swing arm assembly 132, a first moving assembly 133 and a second moving assembly 134.

The swing arm assembly 132 comprises a swing arm 1321 and a lifting rod 1322, the lifting rod 1322 can be vertically lifted and rotatably installed on the installation base 131, the swing arm 1321 is horizontally arranged, one end of the swing arm 1321 is connected to the lifting rod 1322, and the ultrasonic transducer 11 is installed at one end, far away from the lifting rod 1322, of the swing arm 1321. The swing arm assembly 132 is used for driving the ultrasonic transducer 11 and the transmission member 12 to vertically lift and horizontally rotate. In one embodiment, the swing arm 1321 and the lift pin 1322 may also be a unitary structure.

The first moving assembly 133 is a lifting assembly, the first moving assembly 133 includes a lifting motor 1331 and a lifting transmission assembly 1332, the lifting motor 1331 is installed on the installation base 131, the lifting transmission assembly 1332 includes a transmission wheel, a transmission belt, a gear and a rack, the rack is vertically installed on the lifting rod 1322, the gear is rotatably installed on the installation base 131, the gear is engaged with the rack, the lifting motor 1331 is connected with the gear through the transmission wheel and the transmission belt, and the lifting motor 1331 drives the lifting rod 1322 to move up and down through the rack and pinion. In one embodiment, the first moving assembly 133 is a linear motor, and an output shaft of the linear motor is directly connected to the lifting rod 1322, and is also capable of driving the lifting rod 1322 to move up and down.

Second removes subassembly 134 and is the runner assembly, second removes subassembly 134 includes rotation motor 1341 and rotation transmission subassembly 1342, rotation motor 1341 installs on mount pad 131, rotation transmission subassembly 1342 includes drive belt and bull gear, the drive belt is the gear belt, the bull gear suit is on lifter 1322, the bull gear passes through the key-type connection with lifter 1322, lifter 1322 can be relative to bull gear elevating movement, the bull gear is used for driving lifter 1322 to rotate, rotation motor 1341 passes through the drive belt and is connected with the bull gear, rotation motor 1341 is used for driving lifter 1322 to rotate. In one embodiment, the rotation motor 1341 is coupled to the lift rod 1322 via a gear set, and is also capable of driving the lift rod 1322 to rotate.

In one embodiment, the moving device 13 only includes the mounting seat 131, the swing arm assembly 132 and the first moving assembly 133, the ultrasonic device 10 has a lifting function, and the ultrasonic device 10 is used for performing a blending operation on the reaction liquid 104 in the containing cup 103 at a specific blending position 102.

In one embodiment, the second moving assembly 134 may also be a planar moving assembly formed by combining X-axis movement and Y-axis movement, which are respectively realized by two motors, and can also realize the driving of the transferring member 12 to move alternately between the blending positions 102.

Referring to fig. 7, in the present embodiment, the transmission member 12 of the ultrasonic apparatus 10 is directly inserted into the reaction liquid 104 of the containing cup 103. The ultrasonic device 10 has a predetermined frequency and voltage so that the ultrasonic vibration propagates mainly in the axial direction, and the second end surface of the transmission member 12 is an ultrasonic wave emitting surface. During ultrasonic mixing, the second end face of the transmission member 12 emits ultrasonic waves into the reaction liquid 104, an ultrasonic sound field is formed in the reaction liquid 104, and the reaction liquid 104 can form violent liquid flow under the action of the ultrasonic sound field, so that the components in the reaction liquid 104 are mixed uniformly.

In addition to the vibration effect of the ultrasonic wave, the reaction liquid 104 can be uniformly mixed, and the cavitation effect generated by the ultrasonic wave in the liquid can also uniformly disperse some agglomerated and adhered substances in the reaction liquid 104. When the frequency and the sound pressure of the ultrasonic waves are controlled and the amplification effect of the transmission member 12 is combined, the ultrasonic energy entering the reaction liquid 104 of the containing cup 103 is greater than the threshold value of the ultrasonic cavitation, and the ultrasonic cavitation phenomenon can be generated in the reaction liquid 104 in the ultrasonic uniformly mixing process. When ultrasonic cavitation occurs, a large amount of energy is released, certain acting force is generated on some agglomerated and adhered substances in the reaction liquid 104, so that the agglomerated and adhered substances are dispersed, and meanwhile, the substances can be uniformly dispersed in the reaction cup under the action of ultrasonic vibration and uniform mixing.

In one embodiment, the ultrasonic device 10 is a non-contact ultrasonic mixing device, the ultrasonic device 10 is in contact with the containing cup 103, and the ultrasonic waves emitted by the ultrasonic device 10 are transmitted to the reaction liquid in the containing cup 103 through the containing cup 103.

Referring to fig. 8 and 9, the ultrasonic apparatus 10 includes an ultrasonic transducer 11 and a transmission member 12. When the ultrasonic mixing is performed, the second end of the transmission member 12 abuts against the outer wall of the containing cup 103, and the ultrasonic vibration is transmitted to the reaction solution 104 through the containing cup 103. Since the transmission element 12 does not need to be inserted into the receiving cup 103, the axial length of the transmission element 12 is shorter than that of a contact transmission element, but it also has the characteristic of gradually or stepwise decreasing from the first end to the second end to achieve an amplification of the amplitude.

When the ultrasonic mixing is performed, the second end face of the transmission member 12 of the present embodiment abuts against the outer wall of the containing cup 103, and the portion of the outer wall of the containing cup 103, which is in contact with the transmission member 12, is a portion surrounding the reaction liquid 104, so as to transmit the ultrasonic vibration generated by the ultrasonic transducer 11 to the liquid in the containing cup 103. The portion of the receiving cup 103 surrounding the reaction solution 104 is the bottom of the receiving cup 103 and the lower end side wall connected to the bottom, so that the second end of the transmission member 12 abuts against the bottom of the receiving cup 103 and any position of the lower end side wall connected to the bottom, and the ultrasonic vibration can be transmitted to the liquid in the receiving cup 103.

In this embodiment, the ultrasonic apparatus 10 is a movable structure, the ultrasonic apparatus 10 further includes a moving device, the moving device includes a mounting seat and a horizontal moving component, the horizontal moving component is mounted on the mounting seat, the ultrasonic transducer is mounted on the horizontal moving component, the horizontal moving component is an air cylinder or a linear motor, and the horizontal moving component is used for driving the second end of the transmission member 12 to abut against or leave the outer wall of the accommodating cup 103 on the blending position 102.

In one embodiment, the ultrasonic device 10 is configured as a fixed structure, and the delivery member 12 is located at a predetermined position, such that after the receiving cup 103 is placed at the blending position 102, the receiving cup 103 will directly contact the second end of the delivery member 12.

In this embodiment, the sample analyzer further includes a holding device 110, and the holding device 110 is used to limit the radial degree of freedom of the containing cup 103 on the blending position 102.

Referring to fig. 10 and 11, the clasping device 110 includes two oppositely disposed clamping assemblies, each clamping assembly includes a clasping motor 111, a clasping cam 112 and a clasping block 113, the clasping motor 111 is mounted on the base 100, an output shaft of the clasping motor 111 is vertically disposed upward, the output shaft of the clasping motor 111 is in transmission connection with the clasping cam 112, the clasping cam 112 is horizontally disposed, the clasping cam 112 is in contact connection with the clasping block 113, the clasping block 113 is horizontally movably mounted on the base 100, and two side surfaces of the clasping block 113 are respectively adapted to the accommodating cup 103 and the clasping cam 112. If the accommodating cup 103 is a circular tube, the surface of the holding clamp block 113 facing the accommodating cup 103 is an inward concave arc surface; if the receiving cup 103 is a square tube, the surface of the clasping clamp block 113 facing the receiving cup 103 is a plane. The convex part of the leading and clasping cam 112 is a convex arc surface, and the clasping clamping block 113 is a concave arc surface with larger curvature towards the clasping cam 112 surface, so that the concave arc surface of the clasping clamping block 113 can guide the convex part of the clasping cam 112 to slide in and slide out. Embrace motor 111 and be used for the drive to embrace cam 112 and rotate to it holds the cup 103 to make to embrace cam 112 and drive and embrace clamp splice 113 and be close to or keep away from, when two bellying of embracing cam 112 all towards holding cup 103 and align on the same line, two embrace clamp splice 113 and will hold cup 103 and embrace, the radial degree of freedom that holds cup 103 is restricted, and then can avoid holding rocking of cup 103 at the supersound mixing in-process, guarantee to hold the good contact between cup 103 and the transmission 12.

In this embodiment, since the ultrasonic device 10 is in contact with the lower end of the containing cup 103 to achieve ultrasonic uniform mixing, the clasping clamping block 113 of the clasping device 110 clasps the side wall of the lower end of the containing cup 103, so as to improve the stability of clasping. When the transmission member 12 of the ultrasonic apparatus 10 abuts against the lower end side wall of the receiving cup 103, the transmission member 12 and the clasping block 113 are arranged to be offset from each other on the lower end side wall of the receiving cup 103.

In an embodiment, the clasping device 110 may also include a linear driving element and a clasping clamping block, the linear driving element is an air cylinder or a linear motor, and the linear driving element drives the clasping clamping block to approach and leave the containing cup 103, so that the limitation of the containing cup 103 can be realized.

The non-contact ultrasonic device 10 is also used in the present embodiment, and can transmit ultrasonic vibration to the reaction liquid 104 in the containing cup 103 to form an ultrasonic sound field and an ultrasonic cavitation phenomenon, so as to ultrasonically mix the reaction liquid 104 in the containing cup 103.

The contact and non-contact ultrasonic device in the embodiment has the following advantages compared with the existing eccentric rotation, shaking and other mechanical mixing mechanisms:

the mixing is more efficient: the mixing energy of the ultrasonic wave is far higher than that of a common mechanical mixing mode, so that the mixing time of the reaction liquid can be greatly shortened, and the overall use time of the immunodetection is improved.

The uniform mixing is more sufficient: the mechanical action force generated during ultrasonic mixing is higher than that of a common mechanical mixing mode, so that the reaction liquid in the reaction cup flows more quickly and sufficiently, and all components can be in full contact.

The components are more uniform and fine after being uniformly mixed: ultrasonic cavitation can uniformly disperse some agglomerated and adhered substances in the reaction liquid, and fully carry out immunoreaction with more detailed size.

In one embodiment, a sample analysis method is provided, which is performed by the sample analyzer of the above-described embodiments.

Referring to fig. 12, in the whole testing process, the sample analyzer mainly includes the following five different testing processes according to different reagent items:

the test process I and the one-step separation are as follows: and respectively adding the sample S and the reagent R, then carrying out primary incubation and primary magnetic separation operation, and then adding the substrate A, incubating and photometry.

Test flow two and two-step method one-step separation: after the sample S is added, adding a reagent R1 in the first step, wherein the reagent R1 is a reagent or a plurality of reagents, and the sample is mixed with the reagent R1 to form a reaction solution for first incubation; after the first incubation, adding a reagent R2 into the mixture in the second step, wherein the reagent R2 is a reagent or a plurality of reagents, and the reagent R2 and the reaction solution after the first incubation form a new reaction solution to perform the second incubation; and performing magnetic separation, adding a substrate A, incubating and photometry on the reaction solution after the second incubation in sequence.

Test flow three and two-step separation: after the sample is filled, the reagent R1 is filled in the first step, the sample and the reagent R1 are mixed to form reaction liquid for first incubation, and first magnetic separation operation is carried out after the first incubation; after the first magnetic separation operation, adding a reagent R2 in the second step, and performing second incubation on a new reaction solution formed by the reagent R2 and the reaction solution subjected to the first magnetic separation; carrying out a second magnetic separation operation on the reaction solution after the second incubation; and (4) sequentially carrying out substrate A filling, incubation and photometry on the reaction solution after the second magnetic separation operation.

Testing process four, sample pretreatment: adding a sample S, and then adding a pretreatment reagent, wherein the pretreatment reagent pretreats the sample to form a sample S'; and adding the reagent R into the pretreated sample S', and then sequentially performing incubation, magnetic separation, substrate A adding, incubation and photometry.

Testing flow five, sample pretreatment: injecting a sample S, and then injecting a diluent, wherein the diluent is used for diluting the sample to obtain a sample S' with lower concentration; and adding a reagent into the diluted sample S', and then sequentially performing incubation, magnetic separation, substrate A adding, incubation and photometry.

In the present embodiment, the sample analysis method is controlled and executed by the controller 60, and the one-step magnetic separation is taken as an example for description. The sample analysis method carries out ultrasonic mixing on the reaction liquid.

Referring to fig. 13, the sample analysis method of the present embodiment includes the following steps:

s101: filling a sample;

the first transfer mechanism 81 transfers the new receiving cup 103 on the cup loading mechanism 71 to the sample loading position 101;

the sample dispensing mechanism 22 suctions the sample S from the sample support mechanism 21, and dispenses the suctioned sample S into the receiving cup 103 on the sample application site 101.

S102: adding a reagent;

the first transfer mechanism 81 transfers the cup 103 containing the sample S from the sample addition site 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the containing cup 103 needing to be added with the reagent R to a reagent adding position;

the reagent dispensing mechanism 32 suctions the reagent R from the reagent holding mechanism 31, and dispenses the suctioned reagent into the receiving cup 103 at the reagent loading position in the reaction mechanism 40, and the sample S and the reagent R in the receiving cup 103 are mixed to form a reaction solution.

S103: ultrasonically mixing uniformly;

the second transfer mechanism 82 transfers the containing cup 103 containing the reaction solution to the mixing position 102;

the ultrasonic device 10 is controlled to perform a mixing operation of the reaction solution in the receiving cup 103 so that the sample S and the reagent R can sufficiently react.

In the ultrasonic mixing process, the ultrasonic device 10 can match different ultrasonic intensities and ultrasonic times according to different test items so as to effectively mix the reaction liquids of different test items.

S104: incubation;

after the completion of the mixing, the second transfer mechanism 82 transfers the containing cup 103 containing the mixed reaction solution from the mixing position 102 back to the inner ring of the reaction mechanism 40, and performs incubation for a predetermined time.

S105: magnetic separation;

the second transfer mechanism 82 transfers the cup 103 containing the incubated reaction solution from the reaction mechanism 40 to the magnetic separation mechanism 50;

the cleaning liquid dispensing structure of the magnetic separation mechanism 50 dispenses the cleaning liquid into the containing cup 103 having the reaction liquid;

the magnetic attraction structure attracts the reaction compound with the magnetic beads in the containing cup 103 through a magnetic field;

the liquid-absorbing structure discharges substances other than the adsorbed reaction complex and liquid out of the receiving cup 103.

S106: filling a substrate;

the substrate dispensing mechanism of the magnetic separation mechanism 50 dispenses the substrate A into the liquid-absorbed receiving cup 103, and the substrate A luminescently labels the reaction mixture in the reaction solution.

S107: incubation;

the second transfer mechanism 82 transfers the cup 103 containing the reaction solution into which the substrate A is injected from the inside of the magnetic separation mechanism 50 back to the outer periphery of the reaction mechanism 40, and incubates.

S108: performing light measurement;

the reaction mechanism 40 transfers the cup 103 containing the incubated reaction solution to the detection site;

the measuring mechanism 90 detects the reaction composition emitted in the containing cup 103;

after the optical measurement is completed, the waste liquid suction mechanism sucks the reaction liquid from the containing cup 103;

the first transfer mechanism 81 transfers the receiving cup 103 from which the reaction solution is discharged, from the reaction mechanism 40 to the cup throwing position 72.

According to the sample analysis method, the ultrasonic device is adopted to perform ultrasonic mixing operation on the reaction liquid, and compared with the traditional mechanical mixing, the ultrasonic mixing can enable the sample and the reagent to be fully reacted, so that the accuracy of a test item is guaranteed.

In one embodiment, the ultrasonic device 10 is further configured to perform ultrasonic mixing on one or more of the sample, the reagent, the reaction solution after incubation, the reaction solution injected with the cleaning solution, and the reaction solution injected with the substrate, so as to improve the accuracy of the item detection.

The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.

Claims (13)

1. A sample analyzer, comprising:

the blending position is used for placing the containing cup;

the ultrasonic device comprises an ultrasonic transducer, a transmission piece and a moving device, wherein the ultrasonic transducer is used for generating ultrasonic vibration, the transmission piece is provided with a first end and a second end, the first end of the transmission piece is connected with the ultrasonic transducer, and the outer diameter of the second end of the transmission piece is smaller than the inner diameter of the accommodating cup; the moving device is connected with the ultrasonic transducer, the moving device is used for driving the ultrasonic transducer and the transmission piece to move relative to the containing cup on the blending position, and the second end of the transmission piece can be inserted into liquid in the containing cup so as to transmit ultrasonic vibration generated by the ultrasonic transducer to the liquid in the containing cup.

2. The sample analyzer of claim 1 wherein the transmission member is a solid structure and the outer diameter of the transmission member decreases gradually or in steps from the first end to the second end.

3. The sample analyzer of claim 1 wherein the first end of the transmission member is threadably or snap-fit with the ultrasonic transducer and the axial end face of the second end of the transmission member is configured to emit ultrasonic waves.

4. The sample analyzer of claim 1 wherein the moving means comprises a mount, a swing arm assembly and a first moving assembly, the swing arm assembly and the first moving assembly being mounted on the mount, the ultrasonic transducer being mounted on the swing arm assembly, the first moving assembly being connected to the swing arm assembly, the first moving assembly being adapted to drive the transfer member into or out of the containment cup.

5. The sample analyzer of claim 4 wherein the moving means further comprises a second moving assembly mounted on the mount, the second moving assembly coupled to the swing arm assembly, the second moving assembly configured to drive the ultrasonic transducer and the transmission member to move in a horizontal direction.

6. The sample analyzer of any one of claims 1 to 5, further comprising a sample carrying mechanism for carrying a sample, a sample dispensing mechanism for aspirating the sample and discharging the sample into the cuvette, a reagent carrying mechanism for carrying a reagent, a reagent dispensing mechanism for aspirating the reagent and discharging the reagent into the cuvette, a reagent dispensing mechanism for performing a mixing operation on a reaction solution formed by mixing the reagent in the cuvette with the sample, a reaction mechanism for providing an incubation site for the reaction solution in the cuvette, and a measurement mechanism for measuring the reaction solution.

7. A sample analyzer, comprising:

the blending position is used for placing the containing cup;

and an ultrasonic device comprising an ultrasonic transducer and a transmission member, the ultrasonic transducer being configured to generate ultrasonic vibrations, the transmission member having a first end and a second end, the first end of the transmission member being connected to the ultrasonic transducer; the second end of the transmission piece is used for abutting against the outer wall of the containing cup positioned on the blending position, and the part of the outer wall of the containing cup, which is in contact with the transmission piece, is a part surrounding the reaction liquid so as to transmit the ultrasonic vibration generated by the ultrasonic transducer into the liquid in the containing cup.

8. The sample analyzer of claim 7 further comprising a clasping means for gripping the containment cup.

9. The sample analyzer as claimed in claim 8, wherein the clasping device comprises two oppositely arranged clamping components, each clamping component comprises a clasping motor, a clasping cam and a clasping clamping block, the clasping motor is in transmission connection with the clasping cam, the clasping cam is in contact connection with the clasping clamping block, and the clasping motor is used for driving the clasping cam to rotate, so that the clasping cam drives the clasping clamping block to be close to or far away from the containing cup.

10. The sample analyzer of claim 7 wherein the transmission member is a solid structure, and the outer diameter of the transmission member decreases gradually or in steps from the first end to the second end.

11. The sample analyzer of claim 7 wherein the axial end face of the second end of the transmission member is configured to emit ultrasonic waves.

12. The sample analyzer of claim 7 wherein the ultrasonic device further comprises a moving device coupled to the ultrasonic transducer, the moving device configured to drive the second end of the transfer member against or away from the containment cup at the mixing position.

13. The sample analyzer of any one of claims 7 to 12, further comprising a sample carrying mechanism for carrying a sample, a sample dispensing mechanism for aspirating the sample and discharging the sample into the cuvette, a reagent carrying mechanism for carrying a reagent, a reagent dispensing mechanism for aspirating the reagent and discharging the reagent into the cuvette, a reagent dispensing mechanism for performing a mixing operation on a reaction solution formed by mixing the reagent in the cuvette with the sample, a reaction mechanism for providing an incubation site for the reaction solution in the cuvette, and a measurement mechanism for measuring the reaction solution.

Images