CN114518464A

CN114518464A - Sample analyzer and sample analyzing method

Info

Publication number: CN114518464A
Application number: CN202011307880.9A
Authority: CN
Inventors: 代剑东; 孙娟娟; 王长安
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2020-11-19
Filing date: 2020-11-19
Publication date: 2022-05-20

Abstract

The sample analyzer comprises a blending position, a blending device and a controller, wherein the blending position is used for placing a containing cup, the blending device is used for performing blending operation on liquid to be blended placed in the containing cup of the blending position, the blending device comprises at least two blending mechanisms, the controller is used for controlling one or at least two blending mechanisms to perform blending operation on the liquid to be blended in the containing cup, and when the at least two blending mechanisms perform blending operation on the liquid to be blended in the containing cup, blending modes of the blending mechanisms are different correspondingly. Because the mixing device on the sample analyzer comprises at least two mixing mechanisms, the mixing mechanisms are used for executing different mixing modes, so that the mixing operation of the liquid to be mixed can be executed by matching one corresponding mixing mechanism or at least two mixing mechanisms aiming at different test items, effective mixing can be realized, and the detection accuracy of different test items can be ensured.

Description

Sample analyzer and sample analyzing method

Technical Field

The invention relates to in-vitro detection equipment, in particular to a sample analyzer and a sample analysis method.

Background

In vitro diagnosis refers to products and services for determining diseases or body functions by detecting human body samples, such as blood, urine, etc., to obtain clinical diagnosis information. Since in vitro diagnostic modalities allow rapid and accurate diagnosis in the early stages of disease, they play an increasingly important role in the field of clinical medicine and related medical research.

In the process of detecting a human body sample, generally, a sample to be detected and a corresponding reagent are mixed to form a reaction solution, and then a mixing device is used for mixing the reaction solution, so that the sample and the reagent can react fully. Traditional mixing device adopts single mixing mechanism more and carries out the mixing operation to reaction liquid, only can carry out effective mixing to specific test item, can't carry out effective mixing to other test items, can't satisfy different test item's mixing demand to can't guarantee the accuracy that different test items detected.

Disclosure of Invention

An embodiment provides a sample analyzer comprising:

the blending position is used for placing the containing cup;

the blending device is used for performing blending operation on the liquid to be blended in the containing cup of the blending position and comprises at least two blending mechanisms; and

the controller is connected with the blending device and used for controlling one or at least two blending mechanisms to perform blending operation on the liquid to be blended in the containing cup, and when the at least two blending mechanisms perform blending operation on the liquid to be blended in the containing cup, blending modes of the blending mechanisms correspondingly performed by the blending mechanisms are different.

In one embodiment, the blending device comprises an ultrasonic blending mechanism, and the ultrasonic blending mechanism is used for transmitting ultrasonic waves into the liquid to be blended in the containing cup.

In one embodiment, the ultrasonic blending mechanism comprises an ultrasonic transducer, a transmission piece and a moving device, wherein the ultrasonic transducer is used for forming ultrasonic vibration, the transmission piece is provided with a first end and a second end, the first end of the transmission piece is connected with the ultrasonic transducer, and the outer diameter of the second end of the transmission piece is smaller than the inner diameter of the accommodating cup; the moving device is connected with the ultrasonic transducer, the moving device is used for driving the ultrasonic transducer and the transmission piece to move relative to the accommodating cup, and the second end of the transmission piece can be inserted into the liquid to be mixed in the accommodating cup so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the liquid to be mixed in the accommodating cup.

In one embodiment, the ultrasonic blending mechanism comprises an ultrasonic transducer and a transmission piece, wherein the ultrasonic transducer is used for forming ultrasonic vibration, the transmission piece is provided with a first end and a second end, and the first end of the transmission piece is connected with the ultrasonic transducer; the second end of the transmission piece is used for abutting against the outer wall of the containing cup, and the part of the outer wall of the containing cup, which is contacted with the transmission piece, is a part surrounding the liquid to be mixed so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the liquid to be mixed in the containing cup.

In one embodiment, the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or is reduced in a stepped manner from the first end to the second end.

In one embodiment, the blending device further comprises a vortex blending mechanism, and the vortex blending mechanism is used for driving the containing cup to rotate eccentrically.

In one embodiment, a cup seat for placing the containing cup is installed on the vortex blending mechanism, and the ultrasonic blending mechanism and the vortex blending mechanism respectively or jointly perform blending operation on the liquid to be mixed in the containing cup on the cup seat.

In one embodiment, the vortex mixing mechanisms are provided with a plurality of vortex mixing mechanisms, and the ultrasonic mixing mechanism can move among the vortex mixing mechanisms so as to respectively perform ultrasonic mixing operation on the liquid to be mixed in the containing cup on the cup base of each vortex mixing mechanism.

In one embodiment, the controller is configured to obtain test item parameters, match one or at least two blending modes from multiple preset blending modes according to the test item parameters, and control corresponding blending mechanisms to perform blending operations.

In one embodiment, the sample analyzer further includes a sample carrying mechanism, a sample dispensing mechanism, a reagent carrying mechanism, a reagent dispensing mechanism, a reaction mechanism, and a measurement mechanism, wherein the sample carrying mechanism is configured to carry a sample, the sample dispensing mechanism is configured to suck the sample and discharge the sample into the receiving cup, the reagent carrying mechanism is configured to carry a reagent, the reagent dispensing mechanism is configured to suck the reagent and discharge the reagent into the receiving cup, the mixing device is configured to perform a mixing operation on a reaction solution formed by mixing the reagent and the sample in the receiving cup, the reaction mechanism is configured to provide an incubation place for the reaction solution in the receiving cup, and the measurement mechanism is configured to measure the reaction solution.

In one embodiment, the sample analyzer further comprises a transfer mechanism, the mixing position is arranged outside the reaction mechanism, and the transfer mechanism is used for transferring the accommodating cup between the reaction mechanism and the mixing position.

In one embodiment, a sample analysis method is provided, comprising the steps of:

placing the containing cup on the mixing position;

and controlling the corresponding blending mechanism to perform blending operation on the liquid to be blended in the containing cup according to the matched blending mode or at least two blending modes.

In one embodiment, the blending mode is selected by:

acquiring test project parameters;

and matching one or at least two blending modes from a plurality of preset blending modes according to the test item parameters.

In one embodiment, the blending mode comprises an ultrasonic blending mode and/or a vortex blending mode, the ultrasonic blending mode is used for transmitting ultrasonic waves into the liquid to be mixed in the containing cup, and the vortex blending mode is used for blending the liquid to be mixed in the containing cup through eccentric rotation.

In one embodiment, the blending mode comprises a plurality of ultrasonic blending modes, and the plurality of ultrasonic blending modes respectively have different ultrasonic intensities and/or ultrasonic action times

In one embodiment, the blending mode comprises a plurality of vortex blending modes, and the plurality of vortex blending modes have different blending time lengths.

In one embodiment, the liquid to be mixed in the holding cup comprises a reaction liquid formed by the sample and the reagent.

In one embodiment, the method further comprises the following steps before the blending operation is performed:

a sample dispensing mechanism and a reagent dispensing mechanism respectively inject a sample and a reagent into the containing cup to form a reaction liquid;

the method also comprises the following steps after the blending operation is executed:

the transfer mechanism transfers the accommodating cup after the mixing operation into the reaction mechanism, and the reaction solution is incubated on the reaction mechanism;

the transfer mechanism transfers the containing cup into the magnetic separation mechanism, and the magnetic separation mechanism performs magnetic separation operation on the incubated reaction liquid;

the magnetic separation mechanism injects the substrate into the reaction liquid after magnetic separation;

the transfer mechanism transfers the containing cup into the reaction mechanism, and the reaction solution injected with the substrate is incubated on the reaction mechanism;

the reaction means transfers the holding cup to a detection position, and the measuring means optically measures the reaction solution.

According to the sample analyzer and the sample analysis method of the embodiment, the blending device on the sample analyzer comprises at least two blending mechanisms, and the blending mechanisms are used for executing different blending modes, so that the corresponding blending mechanism or mechanisms can be matched with different test items to execute blending operation on the liquid to be blended, effective blending is realized, and the detection accuracy of different test items can be ensured.

Drawings

FIG. 1 is a schematic diagram of an embodiment of an apparatus for analyzing an immune luminescence;

FIG. 2 is a block diagram showing the structure of a control section of an immunofluorescence analyzer in an embodiment;

FIG. 3 is a schematic structural diagram of a contact ultrasonic mixing mechanism in one embodiment;

FIG. 4 is a structural view of a transmission member in one embodiment;

FIG. 5 is a structural view of a transmission member in one embodiment;

FIG. 6 is a diagram illustrating a mobile device according to an embodiment;

FIG. 7 is a schematic diagram of an ultrasonic blending mechanism in one embodiment;

FIG. 8 is a schematic structural diagram of a non-contact ultrasonic mechanism in one embodiment;

FIG. 9 is a schematic diagram of a non-contact ultrasonic mechanism in one embodiment;

FIG. 10 is a side view of a clasping means in an embodiment;

FIG. 11 is a top view of a clasping means in one embodiment;

FIG. 12 is a schematic diagram of the mechanism of the vortex mixing mechanism in one embodiment;

FIG. 13 is a timing diagram of a sample analysis method in one embodiment;

FIG. 14 is a flow diagram of a method of sample analysis in one embodiment;

FIG. 15 is a flow chart of ultrasonic blending in one embodiment;

FIG. 16 is a flow diagram of a method for sample analysis in one embodiment.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments have been given like element numbers associated therewith. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the described features, operations, or characteristics may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like elements associated therewith. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in this specification in order not to obscure the core of the present application with unnecessary detail, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for clarity of description of certain embodiments only and are not meant to be required unless otherwise indicated where a certain sequence must be followed.

For an immune luminescence analyzer, the one-step test item in the invention means that one test item only needs one-step incubation; accordingly, a multi-step test item refers to a test item requiring multiple incubations, for example, a two-step test item refers to a test item requiring two incubations, where the reagents required for the first incubation are added to the sample, followed by the first incubation, and after the first incubation time is reached, the reagents required for the second incubation are added, followed by the second incubation, and after the second incubation time is reached, magnetic separation is performed again, and then the assay is performed. Generally, a multi-step test procedure requires magnetic separation after the final incubation step is completed before the assay can be performed; in a multi-step method test item, except the last step of incubation, whether magnetic separation is needed after other steps of incubation depends on the type of the test item and other factors. For example, a two-step test item may be referred to as a two-step two-separation test item if magnetic separation is required after incubation in the first step of the test, or as a two-step one-separation test item if magnetic separation is not required after incubation in the first step of the test.

In the one-step test item or the multi-step test item, the type of the reagent to be added in each step of incubation or each time of incubation can be one or more, which is determined according to the type of the test item and other factors; when the incubation of the test is carried out in plural kinds of reagents in one-step test items or multi-step test items, such test items may be referred to as multi-component test items.

The embodiment of the invention provides a sample analyzer, wherein a blending device is arranged in the sample analyzer, the blending device comprises a plurality of blending mechanisms, one or at least two blending mechanisms are matched for different test items, and blending operation is carried out on liquid to be blended so as to improve the detection accuracy. The sample analyzer may be a biochemical analyzer or an immunoassay analyzer, and the present embodiment takes an immunoassay analyzer as an example for explanation.

Referring to fig. 1 and 2, the immunofluorescence analyzer mainly includes a mixing device, a sample holding mechanism 21, a sample dispensing mechanism 22, a reagent holding mechanism 31, a reagent dispensing mechanism 32, a reaction mechanism 40, a magnetic separation mechanism 50, and a controller 60. The sample support mechanism 21, the sample dispensing mechanism 22, the reagent support mechanism 31, the reagent dispensing mechanism 32, the reaction mechanism 40, the magnetic separation mechanism 50, and the kneading apparatus are all mounted on the base 100, the controller 60 is mounted on the main body of the base 100, and the controller 60 may also be mounted on the base 100. The biochemical analyzer does not include the above-described magnetic separation mechanism.

The immune luminescence analyzer further comprises a cup loading mechanism 71, a cup throwing position 72, a first transfer mechanism 81, a second transfer mechanism 82 and a measuring mechanism 90 which are arranged on the base 100.

Wherein, the reaction mechanism 40 is arranged in the middle, and the reagent bearing mechanism 31, the magnetic separation mechanism 50, the ultrasonic blending mechanism 10, the cup feeding mechanism 71, the cup throwing position 72 and the measuring mechanism 90 are respectively arranged around the reaction mechanism 40.

The cup loading mechanism 71 is used for storing a new used receiving cup 103, and the receiving cup 103 is also called a reaction cup. The cup loading mechanism 71 itself also has a cup moving function, and can move the storage cup 103 from the storage position to a position to be gripped.

The first transfer mechanism 81 is disposed between the upper cup mechanism 71 and the reaction mechanism 40, the first transfer mechanism 81 is a cup grasping mechanism, and the first transfer mechanism 81 is used for transferring a new receiving cup 103 on the upper cup mechanism 71 to the sample application site 101 near the reaction mechanism 40 and transferring the receiving cup 103 on the sample application site 101 to the reaction mechanism 40.

The cup throwing position 72 is located in the moving range of the first transfer mechanism 81, the cup throwing position 72 is connected with the recycling box, and the cup throwing position 72 is used for recycling the used accommodating cup 103. The first transfer mechanism 81 is also used for transferring the detected containing cup 103 on the reaction mechanism 40 to the cup throwing position 72.

The sample support mechanism 21 is used for supporting a sample. The Sample support mechanism 21 may include, in some examples, a Sample Delivery Module (SDM); in other examples, the sample carrier mechanism 21 may be a sample tray including a plurality of sample sites for placing samples, such as sample tubes, and the sample tray may be configured to rotate to dispatch the samples to corresponding locations, such as locations for the sample dispensing mechanism 22 to aspirate the samples.

The sample dispensing mechanism 22 includes a sampling needle, a moving mechanism for driving the sampling needle to move between the sample carrying mechanism 21 and the sample application site 101, and a drive pump for providing the sampling needle with power for sucking and discharging samples. The sample dispensing mechanism 22 is used for sucking the sample in the sample tube on the sample carrying mechanism 21 and for dispensing the sucked sample into the receiving cup 103 on the dispensing position 101.

The reagent carrying mechanism 31 is for carrying a reagent. In one embodiment, the reagent carrying mechanism 31 may be a reagent disk, the reagent disk is configured in a disk-shaped structure and has a plurality of positions for carrying reagent containers, and the reagent carrying mechanism 31 can rotate and drive the reagent containers carried by the reagent carrying mechanism 31 to rotate to a specific position, for example, a position for sucking reagent by the reagent dispensing mechanism 32. The number of the reagent carrying mechanism 31 may be one or more.

The reagent dispensing mechanism 32 includes a reagent needle, a moving mechanism for driving the reagent needle to move two-dimensionally or three-dimensionally between the reagent holding mechanism 31 and the reaction mechanism 40, and a drive pump for supplying power to the reagent needle for sucking and discharging a reagent. The reagent dispensing mechanism 32 is used for sucking the reagent in the reagent tube on the reagent bearing mechanism 31 and for filling the sucked reagent into the accommodating cup 103 provided with the sample on the reaction mechanism 40, and the sample and the reagent in the accommodating cup 103 are mixed and reacted to form a reaction liquid.

The reaction mechanism 40 is used for providing an incubation place for the reaction solution, the reaction mechanism 40 can be a reaction disc which is arranged in a disc-shaped structure and provided with one or more placing positions for placing reaction cups, and the reaction disc can rotate and drive the reaction cups in the placing positions to rotate and is used for scheduling the reaction cups in the reaction disc and incubating the reaction solution in the reaction cups.

The magnetic separation mechanism 50 comprises a cleaning solution dispensing structure, a magnetic absorption structure, a liquid absorption structure and a substrate dispensing structure, wherein the cleaning solution dispensing structure is used for dispensing a cleaning solution into the incubated reaction solution, and the cleaning solution is used for separating free substances in the incubated reaction solution; the magnetic attraction structure is used for performing magnetic attraction operation on the reaction liquid filled with the cleaning liquid, and is used for adsorbing a reaction compound combined with the magnetic beads; the liquid-absorbing structure is used for discharging the components other than the reaction complex bound to the magnetic beads out of the receiving cup 103; the substrate dispensing mechanism is used to dispense a substrate into the reaction solution in the receiving cup 103, and the substrate reacts with the reaction complex in the reaction solution, and the substrate labels the reaction complex by light emission.

The number of the magnetic separation mechanisms 50 is two, and the two magnetic separation mechanisms 50 can work independently of each other to improve the testing efficiency.

The second transfer mechanism 82 is installed between the reaction mechanism 40 and the magnetic separation mechanism 50, a mixing position 102 is arranged at a position close to the reaction mechanism 40 and the magnetic separation mechanism 50, a cup seat for placing the containing cup 103 is arranged at the mixing position 102 and the sample adding position 101, and the second transfer mechanism 82 is used for transferring the containing cup 103 among the reaction mechanism 40, the magnetic separation mechanism 50 and the mixing position 102.

The measurement means 90 is used to perform optical measurement on the reaction solution after completion of incubation, and obtain reaction data of the sample. For example, the measuring unit 90 detects the light emission intensity of the reaction solution to be measured, and calculates the concentration of the component to be measured in the sample from the calibration curve.

The base 100 is further provided with a cleaning mechanism and a waste liquid suction mechanism, the cleaning mechanism is used for cleaning the sampling needle and the reagent needle, and the waste liquid suction mechanism is used for sucking reaction liquid after detection.

In this embodiment, the blending device includes two blending mechanisms, and the two blending mechanisms are respectively an ultrasonic blending mechanism 10 and a vortex blending mechanism 200. The ultrasonic blending mechanism 10 is used for transmitting ultrasonic waves into the reaction liquid in the containing cup 103 and performing ultrasonic blending operation on the reaction liquid; the vortex mixing mechanism 200 is used for driving the accommodating cup 103 to eccentrically rotate, so that the reaction liquid in the accommodating cup 103 forms vortex rotation, and vortex mixing operation is performed on the reaction liquid.

The ultrasonic blending mechanism 10 and the vortex blending mechanism 200 are in a mutual replaceable relationship, and the working positions of the ultrasonic blending mechanism and the vortex blending mechanism are the same, so that the ultrasonic blending mechanism and the vortex blending mechanism occupy the same period in the whole machine test process. The ultrasonic blending mechanism 10 is a movable mechanism, wherein one of the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 does not work and does not interfere with each other mechanically or conflict with each other in time, and the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 can also work simultaneously and do not interfere with each other.

The ultrasonic blending mechanism 10 is arranged close to the blending position 102, the vortex blending mechanism 200 is arranged at the blending position 102, and the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 are respectively used for performing blending operation on the reaction liquid of the containing cup 103 on the blending position 102.

And performing a mixing operation on the reaction solution at the mixing position 102, where the reaction solution includes different solutions in the detection process, such as a reaction solution including a reaction solution before incubation, a reaction solution after incubation, a reaction solution into which a substrate is injected, and the like.

In one embodiment, at least two mixing positions 102 are provided, the placing positions of the containing cups 103 are increased, one ultrasonic mixing mechanism 10 corresponds to at least two mixing positions 102, that is, one ultrasonic mixing mechanism 10 can perform ultrasonic mixing on reaction liquid 104 in a plurality of containing cups 103 respectively, one containing cup 103 can perform transfer or other operations in the ultrasonic mixing process, the containing cups 103 on other mixing positions 102 can perform transfer or other operations, and the containing cups 103 on the mixing positions 102 realize alternate mixing, so that the detection efficiency is improved.

In one embodiment, the ultrasonic blending mechanisms 10 may also be arranged in one-to-one correspondence with the blending positions 102.

In one embodiment, the blending devices are disposed at positions other than the blending position 102, and the blending devices respectively perform blending operations on different liquids to be blended. If a blending device is arranged at the sample adding position 101, the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 are used for performing blending operation on the sample in the containing cup 103 on the sample adding position 101, so that all components in the sample are uniformly dispersed, and the full reaction of the sample and the reagent is facilitated. For another example, a blending device is installed in the magnetic separation mechanism 50, and the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 are used for performing blending operation on the reaction liquid injected with the cleaning liquid or the reaction liquid injected with the substrate in the receiving cup 103 in the magnetic separation mechanism 50, so as to improve the detection accuracy.

In this embodiment, the controller 60 is connected to the ultrasonic kneading mechanism 10, the vortex kneading mechanism 200, the sample dispensing mechanism 22, the reagent dispensing mechanism 32, the reaction mechanism 40, the magnetic separation mechanism 50, the first transfer mechanism 81, and the second transfer mechanism 82, respectively, and the controller 60 is configured to control the test sequence of the entire sample analyzer.

When the controller 60 controls the mixing device, the controller 60 obtains a test item input or selected by a doctor, obtains a test item parameter corresponding to the test item, and matches one mixing mode from a plurality of mixing modes according to the test item parameter to perform mixing operation on the reaction solution.

Different blending modes have different blending mechanisms, different blending strengths or different blending times respectively, wherein the blending mechanism independently adopts the ultrasonic blending mechanism 10 to execute ultrasonic blending, or independently adopts the vortex blending mechanism 200 to execute vortex blending, or adopts the combination of the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 to execute blending, the blending strength is controlled by input power, at least three blending strengths of strength, strength and weakness can be set, and at least two blending times of 1s and 2s can be set.

The blending mode at least comprises the following steps:

in the first blending mode, a vortex blending mechanism 200 is adopted to perform ultrasonic blending operation, the blending intensity is weak, and the blending time is 1 s;

in the second blending mode, the ultrasonic blending operation is executed by adopting a vortex blending mechanism 200, the blending intensity is weak, and the blending time is 2 s;

in the third blending mode, an ultrasonic blending mechanism 10 is adopted to execute ultrasonic blending operation, the blending intensity is medium, and the blending time is 1 s;

in the fourth blending mode, the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 are adopted to simultaneously execute blending operation, the blending strength of the ultrasonic blending mechanism 10 is weak, the blending time of the ultrasonic blending mechanism 10 is 2s, the blending strength of the vortex blending mechanism 200 is medium, and the blending time of the vortex blending mechanism 200 is 2 s;

in the fifth blending mode, the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 are adopted to perform blending operation in sequence, the blending intensity of the ultrasonic blending mechanism 10 is strong, the blending time of the ultrasonic blending mechanism 10 is 1s, the blending intensity of the vortex blending mechanism 200 is weak, and the blending time of the vortex blending mechanism 200 is 2 s.

The test item parameters include numbers, letters, or a combination thereof, etc., such as the test item parameter of the TNI (troponin) item of 2, and the test item parameter of the E2 (estradiol) item of 0 and 1. The controller 60 is pre-stored with project test parameters corresponding to different test projects, each corresponding to a blending mode. If the test item parameter 0 corresponds to the first blending mode, when the test item parameter obtained by the controller 60 is 0, the vortex blending mechanism 200 is driven to perform vortex blending with weak intensity and 1s of time on the reaction solution; the test item parameter 1 corresponds to the second blending mode, and when the test item parameter obtained by the controller 60 is 1, the vortex blending mechanism 200 is driven to perform vortex blending with weak intensity and time of 2s on the reaction solution; the test item parameter 2 corresponds to the third blending mode, and when the test item parameter acquired by the controller 60 is operation 2, the ultrasonic blending mechanism 10 is driven to perform ultrasonic blending with the intensity of the reaction liquid and the time of 1 s.

Different blending modes can be set according to specific test items, so that the blending device can effectively blend the reaction liquid in different test items.

Referring to fig. 3, in the present embodiment, the ultrasonic blending mechanism 10 is a device independent of other mechanisms, that is, the ultrasonic blending mechanism 10 can operate independently of the sample dispensing mechanism 22, and the ultrasonic blending mechanism 10 can operate synchronously or asynchronously with other mechanisms, so as to improve the efficiency of item detection.

The ultrasonic mixing mechanism 10 is a contact type ultrasonic mixing mechanism, the ultrasonic mixing mechanism 10 comprises an ultrasonic transducer 11, a transmission piece 12 and a moving device 13, the ultrasonic transducer 11 comprises a back lining layer, a piezoelectric layer and a matching layer which are sequentially connected, the piezoelectric layer is a piezoelectric crystal, the piezoelectric crystal generates compression and expansion in the thickness direction through inverse piezoelectric effect under the action of a driving electric signal, and the frequency of the deformation reaches ultrasonic frequency to form ultrasonic vibration. The ultrasonic transducer 11 is connected with the controller 60, and the controller 60 is used for controlling the output power and the output duration of the ultrasonic transducer 11 so as to realize a plurality of ultrasonic blending modes with different ultrasonic intensities and different ultrasonic durations.

Referring to fig. 3 and 4, the transmission member 12 is a solid rod-shaped structure, the transmission member 12 has a first end and a second end, the first end is an upper end, the second end is a lower end, the first end of the transmission member 12 is provided with an external thread, the lower end of the ultrasonic transducer 11 is provided with an internal thread, the transmission member 12 is installed at the lower end of the ultrasonic transducer 11 by a threaded connection manner, and the transmission member 12 can also be connected with the ultrasonic transducer 11 by other manners such as clamping. The transmission member 12 is a resonant rod, the transmission member 12 is connected to the matching layer of the ultrasonic transducer 11, and the transmission member 12 transmits the ultrasonic vibration. Compared with the transmission member 12 with a hollow structure, the solid transmission member 12 is beneficial to the transmission of axial vibration, and when the outer diameter of the transmission member 12 is reduced along the transmission direction of ultrasonic vibration, the solid transmission member 12 is beneficial to the convergence of energy so as to realize better ultrasonic uniform mixing effect.

The external diameter of the transmission part 12 is gradually reduced or reduced in a step mode from the first end to the second end, the transmission part 12 has the function of energy gathering, when the ultrasonic vibration is transmitted from the first end to the second end, the axial cross-sectional area of the second end relative to the first end is reduced, the ultrasonic vibration is gathered more at the second end relative to the first end, the amplitude of the emergent ultrasonic vibration is enlarged by the second end of the transmission part 12 relative to the first end, and therefore the emergent ultrasonic energy is improved.

Specifically, the transmission member 12 includes a first end 121, an intermediate section 122, and a second end 123, wherein the first end 121 is an externally threaded connecting end, the second end 123 is a needle bar structure, and the second end 123 has an outer diameter smaller than the inner diameter of the receiving cup 103, such that the second end 123 of the transmission member 12 can be inserted into the receiving cup 103. The middle section 122 is of a horn-shaped structure, one end of the middle section 122 connected with the first end 121 is a horn big end, one end of the middle section 122 connected with the second end 123 is a horn small end, and the diameter of the middle section 122 from the horn big end to the horn small end is gradually reduced.

The intermediate section 122 may also be comprised of one or any combination of cylindrical and conical rods. Referring to FIG. 5, the middle section 122 of the a-structure includes two cylindrical rods with different diameters; the middle section 122 of the b-structure comprises four cylindrical rods with different diameters; the middle section 122 of the c-structure comprises a section of conical rod; the intermediate section 122 of the d-configuration comprises two cylindrical rods of different diameters and one conical rod. The five structures of the transmission member 12 are all structures that gradually decrease or step-wise decrease from the first end to the second end, and can play a role in amplifying the amplitude.

Referring to fig. 6, the moving device 13 includes a mounting seat 131, a swing arm assembly 132, a first moving assembly 133 and a second moving assembly 134.

The swing arm assembly 132 comprises a swing arm 1321 and a lifting rod 1322, the lifting rod 1322 can be vertically lifted and rotatably installed on the installation base 131, the swing arm 1321 is horizontally arranged, one end of the swing arm 1321 is connected to the lifting rod 1322, and the ultrasonic transducer 11 is installed at one end, far away from the lifting rod 1322, of the swing arm 1321. The swing arm assembly 132 is used for driving the ultrasonic transducer 11 and the transmission member 12 to vertically lift and horizontally rotate. In one embodiment, the swing arm 1321 and the lift pin 1322 may also be a unitary structure.

The first moving assembly 133 is a lifting assembly, the first moving assembly 133 includes a lifting motor 1331 and a lifting transmission assembly 1332, the lifting motor 1331 is installed on the installation base 131, the lifting transmission assembly 1332 includes a transmission wheel, a transmission belt, a gear and a rack, the rack is vertically installed on the lifting rod 1322, the gear is rotatably installed on the installation base 131, the gear is engaged with the rack, the lifting motor 1331 is connected with the gear through the transmission wheel and the transmission belt, and the lifting motor 1331 drives the lifting rod 1322 to move up and down through the rack and pinion. In one embodiment, the first moving assembly 133 is a linear motor, and an output shaft of the linear motor is directly connected to the lifting rod 1322, and is also capable of driving the lifting rod 1322 to move up and down.

Second removes subassembly 134 and is the runner assembly, second removes subassembly 134 includes rotation motor 1341 and rotation transmission subassembly 1342, rotation motor 1341 installs on mount pad 131, rotation transmission subassembly 1342 includes drive belt and bull gear, the drive belt is the gear belt, the bull gear suit is on lifter 1322, the bull gear passes through the keyway with lifter 1322 and is connected, lifter 1322 can go up and down to move relative to the bull gear, the bull gear is used for driving lifter 1322 to rotate, rotation motor 1341 passes through the drive belt and is connected with the bull gear, rotation motor 1341 is used for driving lifter 1322 to rotate. In one embodiment, the rotation motor 1341 is coupled to the lift rod 1322 via a gear set, and is also capable of driving the lift rod 1322 to rotate.

In one embodiment, the moving device 13 only includes the mounting seat 131, the swing arm assembly 132 and the first moving assembly 133, the ultrasonic device 10 has a lifting function, and the ultrasonic device 10 is used for performing a blending operation on the reaction liquid 104 in the containing cup 103 at a specific blending position 102.

In one embodiment, the second moving assembly 134 may also be a planar moving assembly formed by combining X-axis movement and Y-axis movement, which are respectively realized by two motors, and can also realize the driving of the transferring member 12 to move alternately between the blending positions 102.

Referring to fig. 7, in the present embodiment, the transmission member 12 of the ultrasonic mixing mechanism 10 is directly inserted into the reaction liquid 104 of the containing cup 103. The ultrasonic mixing mechanism 10 has a preset frequency and a preset voltage, so that the ultrasonic vibration is mainly transmitted along the axial direction, and the end surface of the second end of the transmission member 12 is an ultrasonic wave emitting surface. During ultrasonic mixing, the second end face of the transmission member 12 emits ultrasonic waves into the reaction liquid 104, an ultrasonic sound field is formed in the reaction liquid 104, and the reaction liquid 104 can form violent liquid flow under the action of the ultrasonic sound field, so that the components in the reaction liquid 104 are mixed uniformly.

In addition to the vibration effect of the ultrasonic wave, the reaction liquid 104 can be uniformly mixed, and the cavitation effect generated by the ultrasonic wave in the liquid can also uniformly disperse some agglomerated and adhered substances in the reaction liquid 104. When the frequency and the sound pressure of the ultrasonic waves are controlled and the amplification effect of the transmission member 12 is combined, the ultrasonic energy entering the reaction liquid 104 of the containing cup 103 is greater than the threshold value of the ultrasonic cavitation, and the ultrasonic cavitation phenomenon can be generated in the reaction liquid 104 in the ultrasonic uniformly mixing process. When ultrasonic cavitation occurs, a large amount of energy is released, certain acting force is generated on some agglomerated and adhered substances in the reaction liquid 104, so that the agglomerated and adhered substances are dispersed, and meanwhile, the substances can be uniformly dispersed in the reaction cup under the action of ultrasonic vibration and uniform mixing.

In one embodiment, the ultrasonic blending mechanism 10 is a non-contact ultrasonic blending mechanism, the ultrasonic blending mechanism 10 is in contact with the containing cup 103, and the ultrasonic waves emitted by the ultrasonic blending mechanism 10 are transmitted to the reaction liquid in the containing cup 103 through the containing cup 103.

Referring to fig. 8 and 9, the ultrasonic mixing mechanism 10 includes an ultrasonic transducer 11 and a transmission member 12. When the ultrasonic mixing is performed, the second end of the transmission member 12 abuts against the outer wall of the containing cup 103, and the ultrasonic vibration is transmitted to the reaction solution 104 through the containing cup 103. Since the transmission element 12 does not need to be inserted into the receiving cup 103, the axial length of the transmission element 12 is shorter than that of a contact transmission element, but it also has the characteristic of gradually or stepwise decreasing from the first end to the second end to achieve an amplification of the amplitude.

When the ultrasonic mixing is performed, the second end face of the transmission member 12 of the present embodiment abuts against the outer wall of the containing cup 103, and the portion of the outer wall of the containing cup 103, which is in contact with the transmission member 12, is a portion surrounding the reaction liquid 104, so as to transmit the ultrasonic vibration generated by the ultrasonic transducer 11 to the liquid in the containing cup 103. The portion of the receiving cup 103 surrounding the reaction solution 104 is the bottom of the receiving cup 103 and the lower end side wall connected to the bottom, so that the second end of the transmission member 12 abuts against the bottom of the receiving cup 103 and any position of the lower end side wall connected to the bottom, and the ultrasonic vibration can be transmitted to the liquid in the receiving cup 103.

In this embodiment, the ultrasonic mixing mechanism 10 is a movable structure, the ultrasonic mixing mechanism 10 further includes a moving device, the moving device includes a mounting seat and a horizontal movement component, the horizontal movement component is installed on the mounting seat, the ultrasonic transducer is installed on the horizontal movement component, the horizontal movement component is an air cylinder or a linear motor, and the horizontal movement component is used for driving the second end of the transmission part 12 to abut against or leave the outer wall of the cup 103 accommodated on the mixing position 102.

In one embodiment, the ultrasonic mixing mechanism 10 is configured as a fixed structure, and the transferring member 12 is located at a predetermined position, so that after the containing cup 103 is placed on the mixing position 102, the containing cup 103 will directly contact with the second end of the transferring member 12.

In this embodiment, the sample analyzer further includes a holding device 110, and the holding device 110 is used to limit the radial degree of freedom of the containing cup 103 on the blending position 102.

Referring to fig. 10 and 11, the clasping device 110 includes two oppositely disposed clamping assemblies, each clamping assembly includes a clasping motor 111, a clasping cam 112 and a clasping block 113, the clasping motor 111 is mounted on the base 100, an output shaft of the clasping motor 111 is vertically disposed upward, the output shaft of the clasping motor 111 is in transmission connection with the clasping cam 112, the clasping cam 112 is horizontally disposed, the clasping cam 112 is in contact connection with the clasping block 113, the clasping block 113 is horizontally movably mounted on the base 100, and two side surfaces of the clasping block 113 are respectively adapted to the accommodating cup 103 and the clasping cam 112. If the accommodating cup 103 is a circular tube, the surface of the holding clamp block 113 facing the accommodating cup 103 is an inward concave arc surface; if the receiving cup 103 is a square tube, the surface of the clasping clamp block 113 facing the receiving cup 103 is a plane. The convex part of the leading and clasping cam 112 is a convex arc surface, and the clasping clamping block 113 is a concave arc surface with larger curvature towards the clasping cam 112 surface, so that the concave arc surface of the clasping clamping block 113 can guide the convex part of the clasping cam 112 to slide in and slide out. Embrace motor 111 and be used for the drive to embrace cam 112 and rotate to it holds cup 103 to make it holds cam 112 drive to embrace clamp splice 113 tightly and be close to or keep away from, when two bellying of embracing cam 112 all towards holding cup 103 and align on one line, two embrace clamp splice 113 tightly and will hold cup 103 and embrace, the radial degree of freedom that holds cup 103 is restricted, and then can avoid holding rocking of cup 103 at supersound mixing in-process, guarantee to hold the good contact between cup 103 and the transmission 12.

In this embodiment, since the ultrasonic mixing mechanism 10 is in contact with the lower end of the containing cup 103 to realize ultrasonic mixing, the clasping clamping block 113 of the clasping device 110 clasps the side wall of the lower end of the containing cup 103, so as to improve the clasping stability. When the transmission piece 12 of the ultrasonic mixing mechanism 10 abuts against the side wall of the lower end of the containing cup 103, the transmission piece 12 and the holding clamp block 113 are arranged on the side wall of the lower end of the containing cup 103 in a staggered mode.

In an embodiment, the clasping device 110 may also include a linear driving element and a clasping clamping block, the linear driving element is an air cylinder or a linear motor, and the linear driving element drives the clasping clamping block to approach and leave the containing cup 103, so that the limitation of the containing cup 103 can be realized.

In the embodiment, the non-contact ultrasonic uniformly-mixing mechanism 10 is also used to transmit ultrasonic vibration to the reaction liquid 104 in the containing cup 103 to form an ultrasonic sound field and an ultrasonic cavitation phenomenon, so as to ultrasonically mix the reaction liquid 104 in the containing cup 103.

Referring to fig. 12, in this embodiment, the vortex blending mechanism 200 includes a driving motor 201, a transmission belt 202, an eccentric rotating shaft 203, a mounting base 204, and the like, the driving motor 201 is fixedly mounted on the mounting base 204, an output shaft of the driving motor 201 is disposed downward, the eccentric rotating shaft 203 is rotatably mounted on the mounting base 204 through a bearing, the eccentric rotating shaft 203 is vertically disposed, the eccentric rotating shaft 203 has a first section and a second section that are not collinear, the first section is located at a lower position, the second section is located at an upper position, and the first section and the second section of the eccentric rotating shaft 203 are both parallel to the output shaft of the driving motor 201. Install the band pulley respectively on the output shaft of driving motor 201 and the first section of eccentric pivot 203, drive belt 202 is connected on the band pulley of driving motor 201 and eccentric pivot 203, driving motor 201 passes through the eccentric pivot 203 of drive belt 202 drive and rotates, install on the eccentric pivot 203 and be used for placing the cup holder 205 that holds cup 103, and then eccentric pivot 203 can drive the cup 103 eccentric rotation that holds that is located on the cup holder 205, carry out the swirl mixing operation to the reaction liquid that holds in the cup 103. The driving motor 201 is connected with the controller 60, and the controller 60 controls the output power and the output duration of the driving motor 201 so as to realize multiple vortex mixing modes with different intensities and time.

The cup base 205 is a mixing position 102, the ultrasonic mixing mechanism 10 can move to the mixing position 102, and ultrasonic mixing operation is performed on the reaction liquid in the cup 103 contained on the cup base 205, so that the ultrasonic mixing mechanism 10 and the vortex mixing mechanism 200 can perform mixing operation on the reaction liquid in the cup 103 contained on the cup base 205 respectively or together.

In this embodiment, the controller 60 is connected with the ultrasonic mixing mechanism 10 and the vortex mixing mechanism 200, and the controller 60 can control the mixing intensity and the mixing time of the ultrasonic mixing mechanism 10 and the vortex mixing mechanism 200 to combine a plurality of ultrasonic modes, so as to meet the requirement of mixing the reaction solution in different detection items. The analyzer of this embodiment still has stronger fault-tolerance, when wherein in the trouble of one in supersound mixing mechanism 10 and the vortex mixing mechanism 200, can replace by another and carry out the mixing, can guarantee the continuation of test.

In one embodiment, a sample analysis method is provided, which is performed by the sample analyzer of the above-described embodiments.

Referring to fig. 13, in the whole testing process, the sample analyzer mainly includes the following five different testing processes according to different reagent items:

the test process I and the one-step separation are as follows: and respectively adding the sample S and the reagent R, then carrying out primary incubation and primary magnetic separation operation, and then adding the substrate A, incubating and photometry.

Test flow two and two-step method one-step separation: after the sample S is added, adding a reagent R1 in the first step, wherein the reagent R1 is a reagent or a plurality of reagents, and the sample and the reagent R1 are mixed to form a reaction solution for first incubation; after the first incubation, adding a reagent R2 into the mixture in the second step, wherein the reagent R2 is a reagent or a plurality of reagents, and the reagent R2 and the reaction solution after the first incubation form a new reaction solution to perform the second incubation; and performing magnetic separation, adding a substrate A, incubating and photometry on the reaction solution after the second incubation in sequence.

Test flow three and two-step separation: after the sample is filled, the reagent R1 is filled in the first step, the sample and the reagent R1 are mixed to form reaction liquid for first incubation, and first magnetic separation operation is carried out after the first incubation; after the first magnetic separation operation, adding a reagent R2 in the second step, and performing second incubation on a new reaction solution formed by the reagent R2 and the reaction solution subjected to the first magnetic separation; carrying out a second magnetic separation operation on the reaction solution after the second incubation; and (4) sequentially carrying out substrate A filling, incubation and photometry on the reaction solution after the second magnetic separation operation.

Testing process four, sample pretreatment: adding a sample S, and then adding a pretreatment reagent, wherein the pretreatment reagent pretreats the sample to form a sample S'; and adding the reagent R into the pretreated sample S', and then sequentially performing incubation, magnetic separation, substrate A adding, incubation and photometry.

Testing flow five and sample pretreatment: injecting a sample S, and then injecting a diluent, wherein the diluent is used for diluting the sample to obtain a sample S' with lower concentration; and adding a reagent into the diluted sample S', and then sequentially performing incubation, magnetic separation, substrate A adding, incubation and photometry.

In the present embodiment, the sample analysis method is controlled and executed by the controller 60, and the one-step magnetic separation is taken as an example for description. The sample analysis method carries out ultrasonic mixing on the reaction liquid.

Referring to fig. 14, the sample analysis method of the present embodiment includes the following steps:

s101: filling a sample;

the first transfer mechanism 81 transfers the new receiving cup 103 on the cup loading mechanism 71 to the sample loading position 101;

the sample dispensing mechanism 22 suctions the sample S from the sample support mechanism 21, and dispenses the suctioned sample S into the receiving cup 103 on the sample application site 101.

S102: adding a reagent;

the first transfer mechanism 81 transfers the cup 103 containing the sample S from the sample addition site 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the containing cup 103 which needs to be added with the reagent R to a reagent adding position;

the reagent dispensing mechanism 32 suctions the reagent R from the reagent holding mechanism 31, and dispenses the suctioned reagent into the receiving cup 103 at the reagent loading position in the reaction mechanism 40, and the sample S and the reagent R in the receiving cup 103 are mixed to form a reaction solution.

S103: mixing uniformly;

the second transfer mechanism 82 transfers the containing cup 103 containing the reaction solution to the mixing position 102;

the mixing device is controlled to perform a mixing operation of the reaction solution in the receiving cup 103 so that the sample S and the reagent R can be sufficiently reacted.

Referring to fig. 15, in detail, the blending operation further includes the following sub-steps:

s1031: acquiring test item parameters;

the controller 60 acquires a test item input or selected by a doctor, and acquires a test item parameter corresponding to the test item;

different test items comprise different test item parameters, and the test item parameters are used for matching different blending modes. The test item parameters include numbers, letters, or a combination thereof, etc., such as the test item parameter of the TNI (troponin) item of 2, and the test item parameter of the E2 (estradiol) item of 0 and 1.

S1032: matching a uniform mixing mode;

the controller 60 is pre-stored with project test parameters corresponding to different test projects, each corresponding to a blending mode. The controller 60 matches one of the blending modes from the plurality of blending modes according to the acquired project test parameters.

Different blending modes have different mixing mechanisms, different blending strengths or different blending times respectively, wherein the mixing mechanism adopts ultrasonic mixing mechanism 10 alone to execute ultrasonic mixing, or adopts vortex mixing mechanism 200 alone to execute vortex mixing, or adopts the combination of ultrasonic mixing mechanism 10 and vortex mixing mechanism 200 to execute mixing, and the mixing strength is controlled by input power, can set at least including strong and weak three mixing strengths, can set at least including two kinds of blending times of 1s and 2 s.

The blending mode at least comprises the following steps:

in the first blending mode, a vortex blending mechanism 200 is adopted to perform ultrasonic blending operation, the blending strength is weak, and the blending time is 1 s;

in the fifth blending mode, the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 are used to perform blending operation in sequence, the blending strength of the ultrasonic blending mechanism 10 is strong, the blending time of the ultrasonic blending mechanism 10 is 1s, the blending strength of the vortex blending mechanism 200 is weak, and the blending time of the vortex blending mechanism 200 is 2 s.

S1033: and controlling the blending mechanism to execute blending operation.

If the test item parameter 0 corresponds to the first blending mode, when the test item parameter obtained by the controller 60 is 0, the vortex blending mechanism 200 is driven to perform vortex blending with weak intensity and 1s of time on the reaction solution; the test item parameter 1 corresponds to the second blending mode, and when the test item parameter obtained by the controller 60 is 1, the vortex blending mechanism 200 is driven to perform vortex blending with weak intensity and time of 2s on the reaction solution; the test item parameter 2 corresponds to the third blending mode, and when the test item parameter acquired by the controller 60 is operation 2, the ultrasonic blending mechanism 10 is driven to perform ultrasonic blending with the intensity of the reaction liquid and the time of 1 s.

The ultrasonic mixing operation of the ultrasonic mixing mechanism 10 is divided into three steps: the ultrasonic blending mechanism 10 firstly moves the transmission part 12 to the reaction liquid on the blending position 102; the transmission piece 12 transmits ultrasonic waves into the reaction liquid to perform ultrasonic mixing; after blending is complete, the delivery member 12 leaves the blending station 102 to return to the initial position.

The vortex mixing mechanism 200 directly drives the cup base 205 to eccentrically rotate, and the cup base 205 drives the containing cup 103 and the reaction liquid inside the containing cup to eccentrically rotate so as to realize vortex mixing operation on the reaction liquid.

In one embodiment, the controller 60 may also control the ultrasonic mixing mechanism 10 and the vortex mixing mechanism 200 to perform mixing operation on the reaction solution on the mixing position 102 sequentially or simultaneously for other test items. When the ultrasonic blending mechanism 10 and the vortex blending mechanism 200 simultaneously perform blending operation on the reaction liquid in the containing cup 103, the ultrasonic blending mechanism 10 adopts a contact type ultrasonic blending mechanism, the transmission part 12 of the ultrasonic blending mechanism 10 is located at the middle position of the containing cup 103 so as to avoid collision between the transmission part 12 and the containing cup 103 which eccentrically rotates, and the range of the middle position is determined by the inner diameter of the containing cup 103 and the radius of the containing cup 103 which eccentrically rotates.

S104: incubation;

after the completion of the mixing, the second transfer mechanism 82 transfers the containing cup 103 containing the mixed reaction solution from the mixing position 102 back to the inner ring of the reaction mechanism 40, and performs incubation for a predetermined time.

S105: magnetic separation;

the second transfer mechanism 82 transfers the cup 103 containing the incubated reaction solution from the reaction mechanism 40 to the magnetic separation mechanism 50;

the cleaning liquid dispensing structure of the magnetic separation mechanism 50 dispenses the cleaning liquid into the containing cup 103 having the reaction liquid;

the magnetic attraction structure attracts the reaction compound with the magnetic beads in the containing cup 103 through a magnetic field;

the liquid-absorbing structure discharges substances other than the adsorbed reaction complex and liquid out of the receiving cup 103.

S106: filling a substrate;

the substrate dispensing mechanism of the magnetic separation mechanism 50 dispenses the substrate A into the liquid-absorbed receiving cup 103, and the substrate A light-emits and labels the reaction mixture in the reaction solution.

S107: incubation;

the second transfer mechanism 82 transfers the cup 103 containing the reaction solution into which the substrate A is injected from the inside of the magnetic separation mechanism 50 back to the outer periphery of the reaction mechanism 40, and incubates.

S108: performing light measurement;

the reaction mechanism 40 transfers the cup 103 containing the incubated reaction solution to the detection site;

the measuring mechanism 90 detects the reaction composition emitted in the containing cup 103;

after the optical measurement is completed, the waste liquid suction mechanism sucks the reaction liquid from the containing cup 103;

the first transfer mechanism 81 transfers the receiving cup 103 from which the reaction solution is discharged, from the reaction mechanism 40 to the cup throwing position 72.

The sample analysis method of this embodiment can match the mixing mode that corresponds with it according to the test item parameter of different test items and carry out the mixing operation, compares single mixing mode, and this application can carry out effective mixing with the reaction liquid of different test items for sample and reagent can obtain abundant reaction, and then have guaranteed the accuracy of test item.

In one embodiment, the sample application site 101 is disposed within the reaction mechanism 40. In step 101, the first transfer mechanism 81 transfers a new receiving cup 103 on the upper cup mechanism 71 to the sample addition site 101 in the reaction mechanism 40, and the sample dispensing mechanism 22 dispenses a sample into the receiving cup 103 on the sample addition site 101 in the reaction mechanism 40, thereby similarly performing sample addition.

In one embodiment, the kneading station 102 and the vortex kneading mechanism 200 are disposed within the reaction mechanism 40. In step 103, the reaction mechanism 40 rotates the containing cup 103 containing the reaction solution to the mixing position 102, or the reagent adding position and the mixing position 102 are located at the same position, and after the reagent dispensing mechanism 32 adds the reagent, the vortex mixing mechanism 200 performs vortex mixing operation. In step 105, the ultrasonic mixing mechanism 10 transmits ultrasonic waves to the reaction solution incubated on the mixing position 102 in the reaction mechanism 40, and performs ultrasonic mixing operation on the reaction solution. Vortex mixing and ultrasonic mixing can also be realized.

In one embodiment, the mixing station 102 and the vortex mixing mechanism 200 are disposed within the magnetic separation mechanism 50. In step 103, the second transfer mechanism 82 transfers the containing cup 103 containing the reaction solution to the mixing position 102 in the magnetic separation mechanism 50; in step 105, the ultrasonic mixing mechanism 10 transmits ultrasonic waves to the reaction solution incubated at the mixing position 102 in the magnetic separation mechanism 50, and performs ultrasonic mixing operation on the reaction solution. The ultrasonic blending operation can be realized.

In one embodiment, a second transfer mechanism 82 is used to throw the cup, and the cup throwing location 72 is set within the range of travel of the second transfer mechanism 82. In step 108, the second transfer mechanism 82 transfers the receiving cup 103 from which the reaction solution is discharged from the reaction mechanism 40 to the cup throwing position 72. The cup throwing operation can be realized as well.

In one embodiment, a sample analysis method is provided, which is different from the sample analysis method in the above embodiment in that: the step of uniformly mixing the reaction liquid filled with the substrate is added. The sample analysis method is performed by a sample analyzer having two blending positions 102, and for convenience of description, the two blending positions 102 are defined as a first blending position 102 and a second blending position 102.

Referring to fig. 16, the sample analysis method of the present embodiment includes the following steps:

s201: filling a sample;

the sample dispensing mechanism 22 suctions the sample S from the sample support mechanism 21, and dispenses the suctioned sample S into the receiving cup 103 at the sample application site 101.

S202: adding a reagent;

the first transfer mechanism 81 transfers the holding cup 103 containing the homogenized sample S from the sample addition site 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the containing cup 103 needing to be added with the reagent R to a reagent adding position;

S203: mixing uniformly;

the second transfer mechanism 82 transfers the containing cup 103 containing the reaction solution to the first kneading station 102;

the mixing device is controlled to perform a mixing operation of the reaction solution in the holding cup 103 at the first mixing position 102 so that the sample S and the reagent R can be sufficiently reacted.

The operation of mixing the reaction solution is the same as the above embodiment, and different mixing modes are matched for different test items to effectively mix the reaction solution.

S204: incubation;

S205: magnetic separation;

S206: filling a substrate;

S207: mixing uniformly;

the second transfer mechanism 82 transfers the receiving cup 103 containing the reaction solution into which the substrate A is injected from the magnetic separation mechanism 50 to the second mixing position 102.

Controlling the blending device to blend the reaction solution of the substrate A injected into the containing cup 103 on the second blending position 102, so that the substrate A in the reaction solution can fully mark the reaction compound.

The operation of mixing the reaction solution into which the substrate a is injected is the same as that in the above embodiment, and different ultrasonic modes are matched for different test items to mix the reaction solution effectively.

The ultrasonic blending mechanism 10 moves alternately between the first blending position 102 and the second blending position 102, and sequentially performs ultrasonic blending operation on the reaction liquid on the first blending position 102 and the reaction liquid on the second blending position 102.

S208: incubation;

S209: performing light measurement;

In the sample analysis method of the embodiment, the two mixing positions 102 are used for respectively performing mixing operation on the reaction solution and the reaction solution injected with the substrate, so that the reaction solution and the reaction solution injected with the substrate can be respectively and effectively mixed, and the efficiency of the whole machine test can be ensured.

The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.

Claims

1. A sample analyzer, comprising:

the blending position is used for placing the containing cup;

2. The sample analyzer of claim 1 wherein the blending device includes an ultrasonic blending mechanism for emitting ultrasonic waves into the liquid to be blended in the containment cup.

3. The sample analyzer of claim 2, wherein the ultrasonic homogenizing mechanism comprises an ultrasonic transducer, a transmission member and a moving device, the ultrasonic transducer is used for generating ultrasonic vibration, the transmission member is provided with a first end and a second end, the first end of the transmission member is connected with the ultrasonic transducer, and the outer diameter of the second end of the transmission member is smaller than the inner diameter of the accommodating cup; the moving device is connected with the ultrasonic transducer, the moving device is used for driving the ultrasonic transducer and the transmission piece to move relative to the containing cup, and the second end of the transmission piece can be inserted into the liquid to be mixed in the containing cup so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the liquid to be mixed in the containing cup.

4. The sample analyzer of claim 2, wherein the ultrasonic homogenizing mechanism comprises an ultrasonic transducer and a transmission member, the ultrasonic transducer is configured to generate ultrasonic vibrations, the transmission member has a first end and a second end, and the first end of the transmission member is connected to the ultrasonic transducer; the second end of the transmission piece is used for abutting against the outer wall of the containing cup, and the part of the outer wall of the containing cup, which is contacted with the transmission piece, is a part surrounding the liquid to be mixed so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the liquid to be mixed in the containing cup.

5. The sample analyzer of claim 3 or 4 wherein the transmission member is a solid structure, and the outer diameter of the transmission member decreases gradually or in steps from the first end to the second end.

6. The sample analyzer of claim 2 wherein the blending device further comprises a vortex blending mechanism for driving the containment cup to rotate eccentrically.

7. The sample analyzer of claim 6, wherein the vortex blending mechanism is provided with a cup holder for holding a containing cup, and the ultrasonic blending mechanism and the vortex blending mechanism respectively or jointly perform blending operation on the liquid to be blended in the containing cup on the cup holder.

8. The sample analyzer of claim 7, wherein the vortex mixing mechanism is provided in plurality, and the ultrasonic mixing mechanism is movable between the plurality of vortex mixing mechanisms to perform ultrasonic mixing operations on the liquid to be mixed in the containing cup of the cup holder of each vortex mixing mechanism.

9. The sample analyzer of claim 1, wherein the controller is configured to obtain test item parameters, match one or at least two blending modes from a plurality of preset blending modes according to the test item parameters, and control corresponding blending mechanisms to perform blending operations.

10. The sample analyzer as claimed in claim 1, further comprising a sample carrying mechanism for carrying a sample, a sample dispensing mechanism for aspirating the sample and discharging the sample into the receiving cup, a reagent dispensing mechanism for carrying a reagent, a reagent dispensing mechanism for aspirating the reagent and discharging the reagent into the receiving cup, a mixing device for performing a mixing operation on a reaction solution formed by mixing the reagent in the receiving cup with the sample, a reaction mechanism for providing an incubation place for the reaction solution in the receiving cup, and a measuring mechanism for measuring the reaction solution.

11. The sample analyzer of claim 10 further including a transfer mechanism, the mixing station being disposed outside the reaction mechanism, the transfer mechanism being configured to transfer the containment cup between the reaction mechanism and the mixing station.

12. A method of analyzing a sample, comprising the steps of:

placing the containing cup on the mixing position;

13. The sample analysis method of claim 12, wherein the blending mode is selected by:

acquiring test item parameters;

14. The sample analysis method according to claim 12, wherein the kneading mode includes an ultrasonic kneading mode for emitting ultrasonic waves into the liquid to be mixed in the containing cup and/or a vortex kneading mode for kneading the liquid to be mixed in the containing cup by eccentric rotation.

15. The sample analysis method of claim 13, wherein the blending mode comprises a plurality of ultrasonic blending modes, each of the plurality of ultrasonic modes having a different ultrasonic intensity and/or ultrasonic action time.

16. The sample analysis method of claim 13, wherein the blending mode comprises a plurality of vortex blending modes, a plurality of the vortex blending modes having different blending durations.

17. The method for analyzing a sample according to claim 13, wherein the liquid to be mixed in the receiving cup comprises a reaction liquid formed by the sample and the reagent.

18. The sample analysis method of any one of claims 12 to 17, further comprising, prior to performing the blending operation, the steps of: