CN213803845U - Micro-nano aeration type cell culture reactor - Google Patents
Micro-nano aeration type cell culture reactor Download PDFInfo
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- CN213803845U CN213803845U CN202022749390.6U CN202022749390U CN213803845U CN 213803845 U CN213803845 U CN 213803845U CN 202022749390 U CN202022749390 U CN 202022749390U CN 213803845 U CN213803845 U CN 213803845U
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Abstract
A micro-nano aeration type cell culture reactor relates to a cell culture reactor. Aims to solve the problem that the prior cell culture reactor is easy to damage cells. The reactor consists of a tank body, a micro-nano aerator and an impeller, and an oxygen dissolving chamber is arranged at the lower part of a partition plate in the tank body; a vertical shaft is arranged at the center of the bottom of the tank body in the oxygen dissolving chamber, and an impeller is horizontally arranged at the upper end part of the shaft; the air outlet of the micro-nano aerator is connected with one end of an aeration main pipe of the micro-nano aerator, the other end of the aeration main pipe extends into the oxygen dissolving chamber, and an aeration head arranged at the air outlet end of an aeration branch pipe is arranged below the impeller. The utility model discloses a micro-nano aeration machine injects the micro-nano bubble of air into the oxygen dissolving chamber, can not cause the cell damage, and the impeller can disperse micro-nano bubble, has promoted the mass transfer and has dissolved the even of oxygen volume. The utility model is suitable for a cell culture.
Description
Technical Field
The utility model relates to a cell culture reactor.
Background
Animal cell culture enables cell growth and proliferation. Cell culture reactors for animal cell culture typically move cell culture media within the reactor by shaking/oscillating, shaking, stirring, etc., the movement of the cell culture media suspending the cells in suspension and achieving efficient gas exchange. Cell culture reactor is mostly bottom oxygen suppliment gas mode, and it is inhomogeneous or dissolved oxygen volume is inhomogeneous to there is the air flow distribution in the cell culture medium to bubble breakage also can cause cell damage, and the cell culture reactor that adopts the stirring simultaneously also can the damage of the cell that causes of different degree owing to too big shearing force.
SUMMERY OF THE UTILITY MODEL
The utility model discloses a solve the problem that current cell culture reactor is fragile to injure the cell, provide a micro-nano aeration formula cell culture reactor.
The micro-nano aeration type cell culture reactor comprises a tank body, a micro-nano aerator and an impeller; a partition board is horizontally arranged in the middle of the tank body, and an oxygen dissolving chamber is arranged at the lower part of the partition board in the tank body; a vertical shaft is arranged at the center of the bottom of the tank body in the oxygen dissolving chamber, and an impeller is horizontally arranged at the upper end part of the shaft; micro-nano aeration machine sets up at jar external portion, and micro-nano aeration machine's aeration is responsible for one end and is connected micro-nano aeration machine's gas vent, and the other end that the aeration was responsible for stretches into in the oxygen dissolving chamber and be connected with several aeration branch pipes, and the end of giving vent to anger of every aeration branch pipe all is provided with the aeration head, and the aeration head setting is in the below of impeller and the blade of the gas outlet orientation impeller of aeration head.
The utility model discloses principle and beneficial effect do:
the utility model discloses a micro-nano aeration machine injects the micro-nano bubble of air into the oxygen dissolving chamber, and the bubble is between 200-60 mu m, and its particle diameter is little, and the rising speed is slow, can stop for a long time in the culture medium, and the bubble constantly dwindles and finally dissolves at the in-process that rises, consequently can not cause the cell damage. The blade of gas outlet towards the impeller of aeration head, the giving vent to anger of aeration head can drive the impeller rotation, and the impeller rotation can disperse micro-nano bubble and exchange about going on the culture medium in the jar body, has promoted the even of mass transfer and dissolved oxygen volume.
Drawings
Fig. 1 is a schematic structural diagram of the micro-nano aeration type cell culture reactor of the utility model.
Detailed Description
The technical scheme of the utility model is not limited to the specific implementation modes listed below, and also comprises any reasonable combination between the specific implementation modes.
The first embodiment is as follows: the micro-nano aeration type cell culture reactor of the embodiment is composed of a tank body 1, a micro-nano aerator 4 and an impeller 7; a partition board 2 is horizontally arranged in the middle of the tank body 1, and an oxygen dissolving chamber 3 is arranged at the lower part of the partition board 2 in the tank body 1; a vertical shaft 5 is arranged at the center of the bottom of the tank body 1 in the oxygen dissolving chamber 3, and an impeller 7 is horizontally arranged at the upper end part of the shaft 5; micro-nano aeration machine 4 sets up in jar 1 outside, and micro-nano aeration machine 4's aeration is responsible for 8 one end and is connected micro-nano aeration machine 4's gas vent, and aeration is responsible for 8 the other end and stretches into in the oxygen dissolution chamber 3 and be connected with several aeration branch pipe 9, and every aeration branch pipe 9's the end of giving vent to anger all is provided with aeration head 6, and aeration head 6 sets up in impeller 7's below and aeration head 6's gas outlet towards impeller 7's blade.
The second embodiment is as follows: the first difference between the present embodiment and the specific embodiment is: the upper end of the tank body 1 is open, a cover plate 10 is arranged on the open, a sampling port 11 is arranged at the center of the cover plate 10, and a plurality of gas outlets 12 are arranged around the sampling port 11.
The third concrete implementation mode: the present embodiment differs from the first or second embodiment in that: the air port 12 is provided with a cover plate.
The fourth concrete implementation mode: the difference between this embodiment mode and one of the first to third embodiment modes is: the separator 2 is a porous polyvinyl chloride plate.
The fifth concrete implementation mode: the fourth difference between this embodiment and the specific embodiment is that: the pore diameter of the porous polyvinyl chloride plate is 0.5-1 micron.
Example 1:
with reference to fig. 1, the micro-nano aeration type cell culture reactor of the embodiment is composed of a tank 1, a micro-nano aerator 4 and an impeller 7; a partition board 2 is horizontally arranged in the middle of the tank body 1, and an oxygen dissolving chamber 3 is arranged at the lower part of the partition board 2 in the tank body 1; a vertical shaft 5 is arranged at the center of the bottom of the tank body 1 in the oxygen dissolving chamber 3, and an impeller 7 is horizontally arranged at the upper end part of the shaft 5; the micro-nano aerator 4 is arranged outside the tank body 1, one end of an aeration main pipe 8 of the micro-nano aerator 4 is connected with an exhaust port of the micro-nano aerator 4, the other end of the aeration main pipe 8 extends into the oxygen dissolving chamber 3 and is connected with a plurality of aeration branch pipes 9, an aeration head 6 is arranged at the air outlet end of each aeration branch pipe 9, the aeration head 6 is arranged below the impeller 7, and the air outlet of the aeration head 6 faces to blades of the impeller 7; the upper end of the tank body 1 is open, a cover plate 10 is arranged on the open, a sampling port 11 is arranged at the center of the cover plate 10, 2 gas outlets 12 are arranged around the sampling port 11, a cover plate is arranged on the gas outlets 12, the partition plate 2 is a porous polyvinyl chloride plate, and the pore diameter is 0.5 micron.
In the embodiment, the micro-nano aeration machine 4 is used for injecting air micro-nano bubbles into the oxygen dissolving chamber 3, the bubbles are 200-60 microns in size, small in particle size and slow in rising speed, can stay in a culture medium for a long time, and are continuously reduced and finally dissolved in the rising process, so that cell damage is avoided. The gas outlet of aeration head 6 is towards the blade of impeller 7, and aeration head 6 give vent to anger can drive impeller 7 and rotate, and impeller 7 rotates and can disperse micro-nano bubble and exchange about going on the culture medium in jar body 1, has promoted the even of mass transfer and dissolved oxygen volume.
Claims (5)
1. The utility model provides a micro-nano aeration formula cell culture reactor which characterized in that: the micro-nano aeration type cell culture reactor consists of a tank body (1), a micro-nano aerator (4) and an impeller (7); a partition plate (2) is horizontally arranged in the middle of the tank body (1), and an oxygen dissolving chamber (3) is arranged at the lower part of the partition plate (2) in the tank body (1); a vertical shaft (5) is arranged at the center of the bottom of the tank body (1) in the oxygen dissolving chamber (3), and an impeller (7) is horizontally arranged at the upper end part of the shaft (5); micro-nano aeration machine (4) set up in jar body (1) outside, the air vent of micro-nano aeration machine (4) is connected to aeration person in charge (8) one end of micro-nano aeration machine (4), the other end of aeration person in charge (8) stretches into in oxygen dissolving chamber (3) and is connected with several aeration branch pipe (9), the end of giving vent to anger of every aeration branch pipe (9) all is provided with aeration head (6), aeration head (6) set up in the below of impeller (7) and the blade of the gas outlet orientation impeller (7) of aeration head (6).
2. The micro-nano aerated cell culture reactor of claim 1, wherein: the utility model discloses a jar body, including jar body (1), the uncovered be provided with apron (10) on the uncovered, apron (10) center is provided with sample connection (11), is provided with several gas outlet (12) around sample connection (11).
3. The micro-nano aerated cell culture reactor of claim 1, wherein: the air port (12) is provided with a cover plate.
4. The micro-nano aerated cell culture reactor of claim 1, wherein: the separator (2) is a porous polyvinyl chloride plate.
5. The micro-nano aerated cell culture reactor of claim 4, wherein: the pore diameter of the porous polyvinyl chloride plate is 0.5-1 micron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022749390.6U CN213803845U (en) | 2020-11-24 | 2020-11-24 | Micro-nano aeration type cell culture reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022749390.6U CN213803845U (en) | 2020-11-24 | 2020-11-24 | Micro-nano aeration type cell culture reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213803845U true CN213803845U (en) | 2021-07-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022749390.6U Active CN213803845U (en) | 2020-11-24 | 2020-11-24 | Micro-nano aeration type cell culture reactor |
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| Country | Link |
|---|---|
| CN (1) | CN213803845U (en) |
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2020
- 2020-11-24 CN CN202022749390.6U patent/CN213803845U/en active Active
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