CN213803844U - Animal cell culture reactor with gas diffusion membrane - Google Patents
Animal cell culture reactor with gas diffusion membrane Download PDFInfo
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- CN213803844U CN213803844U CN202022743638.8U CN202022743638U CN213803844U CN 213803844 U CN213803844 U CN 213803844U CN 202022743638 U CN202022743638 U CN 202022743638U CN 213803844 U CN213803844 U CN 213803844U
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Abstract
An animal cell culture reactor with a gas diffusion membrane relates to a cell culture reactor. Aims to solve the problem that the cells are easy to be damaged due to the rupture of bubbles in the existing animal cell culture reactor. The reactor consists of an outer tank, strip-shaped blades, an inner tank, a first gas diffusion membrane, a second gas diffusion membrane, a shaft, a gear ring, a motor, a gear, a base, a support, a cross rod, a vertical suspender, a transverse suspender, a cover plate and a gas pipe. The utility model discloses the reactor can refine the bubble and promote simultaneously dissolving, has reduced the cell damage. The rotation of the outer tank can lift the liquid medium between the outer tank and the inner tank. The utility model is suitable for an animal cell culture.
Description
Technical Field
The utility model relates to an animal cell culture reactor.
Background
Animal cell culture refers to the process of taking out relevant tissues from animals, dispersing the tissues into single cells, and adding the single cells into a proper culture medium in a cell culture reactor to perform cell growth and proliferation. Proper temperature, pH, gas environment and the like are required in the animal cell culture process. Air or oxygen is introduced into the cell culture reactor through a gas distributor or a pipeline, so that bubbles are generated in the reactor, and the bubbles rise and break after contacting with cells to damage the cells and influence the culture result of the animal cells. Meanwhile, air or oxygen is introduced into the cell culture reactor at the bottom of the reactor, so that the dissolved oxygen in the whole reactor is not uniform.
SUMMERY OF THE UTILITY MODEL
The utility model discloses a solve the problem that the cell was easily damaged in the bubble breakage among the current animal cell culture reactor, provide an animal cell culture reactor with gas diffusion membrane.
The animal cell culture reactor with the gas diffusion membrane of the utility model consists of an outer tank, strip-shaped blades, an inner tank, a first gas diffusion membrane, a second gas diffusion membrane, a shaft, a gear ring, a motor, a gear, a base, a bracket, a cross bar, a vertical suspender, a horizontal suspender, a cover plate and a gas pipe;
the upper end of the outer tank is provided with an opening, the strip-shaped blades are obliquely arranged on the inner wall of the outer tank, the inner tank is arranged in the outer tank, the inner tank is cylindrical, the opening at the upper end of the inner tank is gradually flared, the upper end of the inner tank is provided with an opening, the upper edge of the inner tank is lower than the upper edge of the outer tank, the lower part of the outer wall of the inner tank is provided with an upward inclined drain pipe, the lower part in the inner tank is horizontally provided with a first gas diffusion film and a second gas diffusion film, the first gas diffusion film is arranged above the second gas diffusion film, the shaft is vertically arranged in the center of the base, the center of the bottom of the outer tank is provided with a shaft sleeve, the shaft sleeve is sleeved at the upper end of the shaft, the gear ring is arranged at the lower end of the outer circumference of the outer tank, the motor is arranged on the base, the gear is arranged on an output shaft of the motor, and the gear is meshed with the gear ring; the support is vertically fixed on the upper surface of the base, the cross rod is horizontally arranged, one end of the cross rod is connected with the upper part of the support through a bolt, the other end of the cross rod is connected with the upper end of a vertically arranged vertical suspender, the transverse suspender is horizontally arranged, two ends of the transverse suspender are fixedly connected with the inner wall of the inner tank, and the lower end of the vertical suspender is connected with the middle part of the transverse suspender; the cover plate is arranged on an opening at the upper end of the outer tank in an overlapping mode, and the lower end of the air pipe penetrates through the cover plate, the first gas diffusion film and the second gas diffusion film in sequence and stretches into the space between the bottom of the inner tank and the outer tank.
The utility model discloses principle and beneficial effect do:
the utility model discloses trachea is to liquid culture medium input gas between inner tank bottom and the outer jar, and first gaseous diffusion barrier and second gaseous diffusion barrier have pore structure, can permeate water and breathe freely, and the bubble that the input gas produced is refined after second gaseous diffusion barrier and first gaseous diffusion barrier in proper order and is promoted simultaneously and dissolve, has reduced the cell damage. The outer jar can rotate under the drive of motor, and the drain pipe is used for the liquid exchange of outer jar and inner tank, and the slope of bar blade sets up on the inner wall of outer jar, and the bar blade can promote the liquid culture medium between outer jar and the inner tank along with the rotation of outer jar, and liquid culture medium flows into the inner tank via the flaring of inner tank upper end, and the flaring flows and has the effect that the inclined plane can play the defoaming.
Drawings
FIG. 1 is a schematic view showing the structure of an animal cell culture reactor with a gas diffusion membrane according to example 1;
fig. 2 is a schematic structural view of the outer tank 1 in embodiment 1.
Detailed Description
The technical scheme of the utility model is not limited to the specific implementation modes listed below, and also comprises any reasonable combination between the specific implementation modes.
The first embodiment is as follows: the animal cell culture reactor with the gas diffusion membrane in the embodiment is composed of an outer tank 1, strip-shaped blades 2, an inner tank 3, a first gas diffusion membrane 5, a second gas diffusion membrane 6, a shaft 7, a gear ring 8, a motor 9, a gear 10, a base 11, a support 12, a cross rod 13, a vertical suspender 14, a transverse suspender 15, a cover plate 16 and a gas pipe 19;
the upper end of the outer tank 1 is an opening, the strip-shaped blades 2 are obliquely arranged on the inner wall of the outer tank 1, the inner tank 3 is arranged in the outer tank 1, the inner tank 3 is cylindrical, the opening at the upper end of the inner tank 3 is a gradually-expanding opening, the upper end of the inner tank 3 is an opening, the upper edge of the inner tank 3 is lower than that of the outer tank 1, the lower part of the outer wall of the inner tank 3 is provided with an upward-inclined drain pipe 4, the lower part of the inner tank 3 is horizontally provided with a first gas diffusion film 5 and a second gas diffusion film 6, the first gas diffusion film 5 is arranged above the second gas diffusion film 6, the shaft 7 is vertically arranged at the center of the base 11, the center of the bottom of the outer tank 1 is provided with a shaft sleeve which is sleeved at the upper end of the shaft 7, the gear ring 8 is arranged at the lower end of the outer circumference of the outer tank 1, the motor 9 is arranged on the base 11, the gear 10 is arranged on the output shaft of the motor 9, and the gear 10 is meshed with the gear ring 8; the support 12 is vertically fixed on the upper surface of the base 11, the cross rod 13 is horizontally arranged, one end of the cross rod is connected with the upper part of the support 12 through a bolt, the other end of the cross rod 13 is connected with the upper end of a vertically arranged vertical suspender 14, the transverse suspender 15 is horizontally arranged, two ends of the transverse suspender are fixedly connected with the inner wall of the inner tank 3, and the lower end of the vertical suspender 14 is connected with the middle part of the transverse suspender 15; the cover plate 16 is arranged on the upper end opening of the outer tank 1, and the lower end of the air pipe 19 penetrates through the cover plate 16, the first gas diffusion film 5 and the second gas diffusion film 6 in sequence and extends into the space between the bottom of the inner tank 3 and the outer tank 1.
In the present embodiment, the gas pipe 19 supplies gas to the liquid culture medium between the bottom of the inner tank 3 and the outer tank 1, the first gas diffusion membrane 5 and the second gas diffusion membrane 6 have a pore structure, and are permeable to water and gas, and bubbles generated by the supplied gas are refined and dissolved simultaneously after passing through the second gas diffusion membrane 6 and the first gas diffusion membrane 5 in sequence, thereby reducing cell damage. Outer jar 1 can rotate under motor 9's drive, and drain pipe 4 is used for outer jar 1 and inner tank 3's liquid exchange, and the slope of bar blade 2 sets up on outer jar 1's inner wall, and bar blade 2 can promote the liquid medium between outer jar 1 and the inner tank 3 along with outer jar 1's rotation, and liquid medium flows into inner tank 3 via the gradual flaring of inner tank 3 upper end, and the flaring flows gradually and has the effect that the inclined plane can play the defoaming.
The second embodiment is as follows: the first difference between the present embodiment and the specific embodiment is: the first gas diffusion film 5 has a volume density of the second gas2-4 times of the bulk diffusion membrane 6; the second gas diffusion membrane 6 had a bulk density of 0.50g/cm3。
The third concrete implementation mode: the present embodiment differs from the first or second embodiment in that: the center of the cover plate 16 is a threaded hole, the center of the cover plate 16 is sleeved on the vertical hanger rod 14, and the cover plate 16 is in threaded connection with the vertical hanger rod 14.
The fourth concrete implementation mode: the difference between this embodiment mode and one of the first to third embodiment modes is: the cover plate 16 is provided with a sampling port 17 and a gas exchange port 18.
The fifth concrete implementation mode: the difference between this embodiment and one of the first to fourth embodiments is: the upper portion of the support 12 is provided with a vertical strip-shaped hole, and a bolt for connecting the cross rod 13 and the support 12 is arranged in the strip-shaped hole.
Example 1: the present embodiment is described with reference to fig. 1 and 2, and the animal cell culture reactor with a gas diffusion membrane of the present embodiment is composed of an outer tank 1, a strip-shaped blade 2, an inner tank 3, a first gas diffusion membrane 5, a second gas diffusion membrane 6, a shaft 7, a gear ring 8, a motor 9, a gear 10, a base 11, a support 12, a cross bar 13, a vertical suspension bar 14, a horizontal suspension bar 15, a cover plate 16 and a gas pipe 19; the upper end of the outer tank 1 is an opening, the strip-shaped blades 2 are obliquely arranged on the inner wall of the outer tank 1, the inner tank 3 is arranged in the outer tank 1, the inner tank 3 is cylindrical, the opening at the upper end of the inner tank 3 is a gradually-expanding opening, the upper end of the inner tank 3 is an opening, the upper edge of the inner tank 3 is lower than that of the outer tank 1, the lower part of the outer wall of the inner tank 3 is provided with an upward-inclined drain pipe 4, the lower part of the inner tank 3 is horizontally provided with a first gas diffusion film 5 and a second gas diffusion film 6, the first gas diffusion film 5 is arranged above the second gas diffusion film 6, the shaft 7 is vertically arranged at the center of the base 11, the center of the bottom of the outer tank 1 is provided with a shaft sleeve which is sleeved at the upper end of the shaft 7, the gear ring 8 is arranged at the lower end of the outer circumference of the outer tank 1, the motor 9 is arranged on the base 11, the gear 10 is arranged on the output shaft of the motor 9, and the gear 10 is meshed with the gear ring 8; the support 12 is vertically fixed on the upper surface of the base 11, the cross rod 13 is horizontally arranged, one end of the cross rod is connected with the upper part of the support 12 through a bolt, the other end of the cross rod 13 is connected with the upper end of a vertically arranged vertical suspender 14, the transverse suspender 15 is horizontally arranged, two ends of the transverse suspender are fixedly connected with the inner wall of the inner tank 3, and the lower end of the vertical suspender 14 is connected with the middle part of the transverse suspender 15; the cover plate 16 is arranged on the upper end opening of the outer tank 1, and the lower end of the air pipe 19 penetrates through the cover plate 16, the first gas diffusion film 5 and the second gas diffusion film 6 in sequence and extends into the space between the bottom of the inner tank 3 and the outer tank 1.
The volume density of the first gas diffusion membrane 5 (non-woven fabric) is 3 times that of the second gas diffusion membrane 6; the second gas diffusion membrane 6 had a bulk density of 0.50g/cm3The nonwoven fabric of (1); the center of the cover plate 16 is a threaded hole, the center of the cover plate 16 is sleeved on the vertical suspender 14, and the cover plate 16 is in threaded connection with the vertical suspender 14; the cover plate 16 is provided with a sampling port 17 and a gas exchange port 18; a vertical strip-shaped hole is formed in the upper part of the support 12, a bolt for connecting the cross rod 13 with the support 12 is arranged in the strip-shaped hole, and the height of the cross rod 13 can be adjusted along with the movement of the bolt; in this embodiment, the gas pipe 19 supplies gas to the liquid culture medium between the bottom of the inner tank 3 and the outer tank 1, the first gas diffusion membrane 5 and the second gas diffusion membrane 6 have a pore structure, and are permeable to water and gas, and bubbles generated by the supplied gas are refined and dissolved simultaneously after passing through the second gas diffusion membrane 6 and the first gas diffusion membrane 5 in sequence, thereby reducing cell damage. Outer jar 1 can rotate under motor 9's drive, and drain pipe 4 is used for outer jar 1 and inner tank 3's liquid exchange, and the slope of bar blade 2 sets up on outer jar 1's inner wall, and bar blade 2 can promote the liquid medium between outer jar 1 and the inner tank 3 along with outer jar 1's rotation, and liquid medium flows into inner tank 3 via the gradual flaring of inner tank 3 upper end, and the flaring flows gradually and has the effect that the inclined plane can play the defoaming.
Claims (5)
1. An animal cell culture reactor with a gas diffusion membrane, which is characterized in that: the animal cell culture reactor with the gas diffusion membrane is composed of an outer tank (1), strip-shaped blades (2), an inner tank (3), a first gas diffusion membrane (5), a second gas diffusion membrane (6), a shaft (7), a gear ring (8), a motor (9), a gear (10), a base (11), a support (12), a cross bar (13), a vertical suspender (14), a transverse suspender (15), a cover plate (16) and a gas pipe (19);
the upper end of the outer tank (1) is an opening, the strip-shaped blades (2) are obliquely arranged on the inner wall of the outer tank (1), the inner tank (3) is arranged in the outer tank (1), the inner tank (3) is cylindrical, the opening at the upper end of the inner tank (3) is gradually flared, the upper end of the inner tank (3) is an opening, the upper edge of the inner tank (3) is lower than the upper edge of the outer tank (1), the lower part of the outer wall of the inner tank (3) is provided with an obliquely inclined drain pipe (4), the lower part of the inner tank (3) is horizontally provided with a first gas diffusion film (5) and a second gas diffusion film (6), the first gas diffusion film (5) is arranged above the second gas diffusion film (6), a shaft (7) is vertically arranged at the center of the base (11), the center of the bottom of the outer tank (1) is provided with a shaft sleeve, the shaft sleeve is sleeved at the upper end of the shaft (7), and a gear ring (8) is arranged at the lower end of the outer tank (1), the motor (9) is arranged on the base (11), the gear (10) is arranged on an output shaft of the motor (9), and the gear (10) is meshed with the gear ring (8); the support (12) is vertically fixed on the upper surface of the base (11), the cross rod (13) is horizontally arranged, one end of the cross rod is connected with the upper part of the support (12) through a bolt, the other end of the cross rod (13) is connected with the upper end of a vertically arranged vertical suspender (14), the transverse suspender (15) is horizontally arranged, two ends of the transverse suspender are fixedly connected with the inner wall of the inner tank (3), and the lower end of the vertical suspender (14) is connected with the middle part of the transverse suspender (15); the cover plate (16) is arranged on an opening at the upper end of the outer tank (1), and the lower end of the air pipe (19) sequentially penetrates through the cover plate (16), the first gas diffusion film (5) and the second gas diffusion film (6) to stretch into the space between the bottom of the inner tank (3) and the outer tank (1).
2. The animal cell culture reactor with gas diffusion membrane of claim 1, characterized in that: the volume density of the first gas diffusion film (5) is 2-4 times that of the second gas diffusion film (6); the second gas diffusion membrane (6) has a bulk density of 0.50g/cm3。
3. The animal cell culture reactor with gas diffusion membrane of claim 1, characterized in that: the center of the cover plate (16) is a threaded hole, the center of the cover plate (16) is sleeved on the vertical suspender (14), and the cover plate (16) is in threaded connection with the vertical suspender (14).
4. The animal cell culture reactor with gas diffusion membrane of claim 1, characterized in that: the cover plate (16) is provided with a sampling port (17) and a gas exchange port (18).
5. The animal cell culture reactor with gas diffusion membrane of claim 1, characterized in that: the upper portion of the support (12) is provided with a vertical strip-shaped hole, and a bolt for connecting the cross rod (13) and the support (12) is arranged in the strip-shaped hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022743638.8U CN213803844U (en) | 2020-11-24 | 2020-11-24 | Animal cell culture reactor with gas diffusion membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022743638.8U CN213803844U (en) | 2020-11-24 | 2020-11-24 | Animal cell culture reactor with gas diffusion membrane |
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| Publication Number | Publication Date |
|---|---|
| CN213803844U true CN213803844U (en) | 2021-07-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022743638.8U Active CN213803844U (en) | 2020-11-24 | 2020-11-24 | Animal cell culture reactor with gas diffusion membrane |
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| Country | Link |
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| CN (1) | CN213803844U (en) |
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2020
- 2020-11-24 CN CN202022743638.8U patent/CN213803844U/en active Active
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