CN213203047U

CN213203047U - Many even bag-type adipose-derived stem cell extraction element

Info

Publication number: CN213203047U
Application number: CN202021638690.0U
Authority: CN
Inventors: 李伟强; 胡旺; 刘亚琼; 邹桥; 刘源
Original assignee: Zhongke Kangsai Biotechnology Chongqing Co ltd
Current assignee: Zhongke Kangsai Biotechnology Chongqing Co ltd
Priority date: 2020-08-07
Filing date: 2020-08-07
Publication date: 2021-05-14
Anticipated expiration: 2030-08-07

Abstract

The utility model discloses a many even pocket type adipose stem cell extraction element, including the first washing bag, second washing bag, third washing bag and the digestion bag that connect gradually, first washing bag, second washing bag, third washing bag and digestion bag between communicate each other, every through the water conservancy diversion hose wash the bag with all be equipped with at least one on the bag body of digestion bag and advance out the appearance mouth add adipose tissue active agent in advance in the first washing bag. The multi-connected-bag type adipose-derived stem cell extraction device provides a relatively closed sterile internal environment for transportation, cleaning and digestion of adipose tissues, reduces the chance of contact with the outside, reduces the probability of adipose tissue pollution, and ensures the safety of experiments; meanwhile, the adipose tissue active agent in the first cleaning bag can play a role in maintaining the adipose tissue activity in the long-time transportation process, so that the adipose tissue cleaning efficiency is further improved.

Description

Many even bag-type adipose-derived stem cell extraction element

Technical Field

The utility model relates to an adipose stem cell field, especially a many even bag-type adipose stem cell extraction element.

Background

Adipose tissue is abundant in human body, a large number of adipose-derived stem cells (ADSCs) obtained by liposuction have self-renewal and proliferation and multidirectional differentiation potential, can be differentiated into adipocytes, chondrocytes, myocytes, osteoblasts, nerve cells, glial cells and islet cells, can secrete various angiogenesis promoting factors and anti-apoptosis factors to resist inflammation and oxidation, can resist damage of oxygen free radicals, and is expected to become a stem cell source for repairing damaged tissues and organs.

At present, most of the collection and transportation of adipose tissues directly select an injector used in liposuction as a carrier, so that the problems of leakage and pollution are very easy to occur in the transportation process, and long-time transportation and uncontrolled external environment are very easy to influence the activity of adipose stem cells. Adipose tissue transports to the laboratory after, and the centrifuging tube that adopts more in the extraction process is as the container, and is repeated to it wash and centrifugation, uses erlenmeyer flask or centrifuging tube to digest adipose tissue as the container after the washing is accomplished, and this washing and digestion process are open application of sample and sample, very easily cause the pollution.

In addition, carry out fat digestion with traditional three-dimensional container and can not all immerse in the water to thermally equivalent in the container can't guaranteeing all the time, again traditional container thermal conductivity is not too good and be difficult for observing, thereby leads to adipose tissue digestion time long, and the inhomogeneous condition of digestion appears.

Disclosure of Invention

The utility model discloses be exactly to current adipose tissue very easily take place to leak and the problem of pollution in transportation, washing and digestion process, and provide a many even bag formula adipose stem cell extraction element, this extraction element makes adipose tissue all be in a relative confined aseptic internal environment in transportation, washing and digestion, has reduced adipose tissue pollution's probability.

In order to achieve the above purpose, the technical scheme of the utility model is as follows: the utility model provides a many even pocket type adipose stem cell extraction element, washs bag, third washing bag and digestion bag including the first washing bag, the second that connect gradually, first washing bag, second are washd and are washd the bag, the third is washd and pass through each other intercommunication, every through the water conservancy diversion hose between bag and the digestion bag wash the bag with all be equipped with at least one on the bag body of digestion bag and advance out the appearance mouth first washing bag is added in advance to have adipose tissue active agent.

The multi-bag fat stem cell extraction device of the utility model is provided with three cleaning bags and a digestion bag, and the whole device keeps a relatively closed sterile environment, so that the cleaning and the digestion of the fat tissue stem cells can be carried out in the closed environment, and the probability of fat tissue pollution is reduced; in addition, the adipose tissue active agent is added in the first cleaning bag in advance, so that the adipose tissues collected in the process of being injected into the first cleaning bag for long-time transportation can protect the activity of the adipose tissues, improve the activity of adipose stem cells, collect and precipitate red blood cells mixed in the adipose tissues, and optimize the cleaning efficiency.

Further, the bag body of digestion bag is the fillet rectangle be equipped with a plurality of strong magnet subsides and attaches the point on the bag body, the bag body passes through strong magnet pastes the point and sets up strong magnet piece to make through the magnetic force of strong magnet piece and constant temperature water bath shaking table the bag body is fixed on the constant temperature water bath shaking table. A plurality of strong magnet pastes the point setting at four angles of the bag body, and the digestion bag is through setting up strong magnet adsorption site on the bag body like this, utilizes the strong magnet piece with the bag body snap-on the constant temperature water bath shaking table, need not to fix with the help of other instruments, just can be so that whole bag body submergence is in constant temperature aquatic for digestion efficiency, improved adipose stem cell's separation extraction efficiency and quality greatly, solved the problem that traditional three-dimensional container can not all submergence aquatic.

Furthermore, the upper part of the digestion bag body is provided with two sample inlets and outlets, one is a digestive juice sample inlet and outlet, and the other sample inlet and outlet is integrally in a branch shape and comprises a main sample inlet and outlet and two branch sample inlets and outlets. Thus, the opening on the bag body is reduced, and the bag body is prevented from being polluted.

Furthermore, a filtering device is arranged on the main sample inlet and outlet. Therefore, when the adipose-derived stem cell suspension is finally extracted, fine adipose tissues can be filtered out, so that the obtained cell suspension can be directly subjected to subsequent operation without additional filtering operation. In addition, this filter equipment is double-deck filter screen structure, and wherein the aperture that is close to the one deck filter screen of the bag body is 300um, and the aperture of another layer filter screen is 100um, and this design can effectively prevent the shutoff when filtering.

Furthermore, moulding metal strips are embedded in the edges of the two sides of the bag body of the first cleaning bag, the second cleaning bag, the third cleaning bag and the digestion bag respectively. This moulding metal strip has certain rigidity intensity, and can take place deformation again, both can prevent like this that the bag body from taking place deformation fold and piling up because of centrifugal force when the bag body is centrifugal, can artificially buckle the metal strip again and drive the bag body and do certain deformation.

Furthermore, each end part of the sample inlet and outlet is provided with a twist-off sealing rubber ring, and each internal interface of the sample inlet and outlet is matched with the nipple interface of the injector. All the sample inlets and outlets are completely sealed in advance, the front end part needs to be twisted off when the sample inlet and outlet bag is used, the sample inlet and outlet operation can be carried out only by exposing the internal interfaces of the sample inlets and outlets, and the inner part of the bag body is in a completely sealed state through the diversion hose connected by heat sealing after the sample inlet and outlet bag is used, so that the risk of pollution is further reduced.

Furthermore, every go into out the appearance mouth through the water conservancy diversion hose with the internal space of the bag body of first washing bag, second washing bag, third washing bag and digestion bag is connected. The sample inlet and outlet can be thermally sealed with the diversion hose close to the end of the bag body after sample adding or sample discharging is completed, and then the used part is removed, so that the bag body is completely isolated from the outside during water bath digestion, and the bag body is prevented from being polluted.

Further, the adipose tissue active agent comprises the following components in percentage by mass: 5500.5-1% of hydroxyethyl starch, 02-0.4% of trehalose, 0.8-1.2% of human serum albumin, 0.1% of tribasic sodium citrate and the balance of PBS buffer solution. So as to protect the activity of the adipose tissues during long-term transportation.

Furthermore, scales are arranged on the surfaces of the first cleaning bag, the second cleaning bag, the third cleaning bag and the digestion bag. Therefore, when the sample feeding and discharging operation is carried out, the judgment of the sample feeding and discharging volume by an operator can be well assisted, and the controllability and the accuracy of the experiment are facilitated.

Furthermore, the diversion hose and the bag body are made of transparent PVC with high resistance, high toughness, heat sealability and strong hydrophobicity and oleophobicity. Therefore, after the adipose tissues are centrifugally layered, the components are clamped and separated layer by layer more smoothly; during water bath, the temperature conduction is faster, and the digestion time of tissues can be shortened; meanwhile, the transparent bag body is convenient for the operation and observation of the whole experimental process. In addition, the diversion hose can be sealed through heat sealing after each operation, used parts are omitted, the encumbrance of redundant parts is reduced, and the sealing performance of the environment in the bag body is guaranteed.

Has the advantages that:

the multi-connected-bag type adipose-derived stem cell extraction device provides a relatively closed sterile internal environment for transportation, cleaning and digestion of adipose tissues, reduces the chance of contact with the outside, reduces the probability of adipose tissue pollution, and ensures the safety of experiments; meanwhile, the adipose tissue active agent in the first cleaning bag can play a role in maintaining the adipose tissue activity in the long-time transportation process, so that the adipose tissue cleaning efficiency is further improved.

Drawings

FIG. 1 is a schematic view of the present invention;

FIG. 2 is a partial schematic view of a sample inlet and outlet;

FIG. 3 is a schematic view of a filtration apparatus;

FIG. 4 is a schematic view of a liquid stop clip.

Labeled as: 1 a first cleaning bag, 2 a second cleaning bag, 3 a third cleaning bag, 4 a digestion bag, 5 a diversion hose, 6 an adipose tissue active agent, 7 a strong magnet attachment point, 8 a digestive juice sample inlet and outlet port, 9 a main sample inlet and outlet port, 10 a branch sample inlet and outlet port, 11 a filtering device, 12 a shaping metal strip, 13 a twist-off sealing rubber ring, 14 an internal interface, 15 scales, 16 a first sample inlet and outlet port, 17 a second sample inlet and outlet port, 18 a third sample inlet and outlet port, 19 a fourth sample inlet and outlet port and 20 a liquid stopping clamp.

Detailed Description

In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

The present invention will be further explained with reference to the drawings and examples.

Example 1

As shown in fig. 1-4: the utility model provides a many even pocket type adipose stem cell extraction element, a serial communication port, including the first washing bag 1, the second that connect gradually wash bag 2, third washing bag 3 and digestion bag 4, first washing bag 1, second wash bag 2, third washing bag 3 and digestion bag 4 between communicate each other through water conservancy diversion hose 5, every wash the bag with all be equipped with at least one sample inlet and outlet on the bag body of digestion bag 4 add adipose tissue active agent 6 in advance in the first washing bag 1.

The digestion bag 4 is characterized in that the bag body is a rounded rectangle, a plurality of strong magnet attachment points 7 are arranged on the bag body, the bag body passes through the strong magnet attachment points 7 and is provided with a strong magnet block, and the bag body is fixed on the constant-temperature water bath rocking bed through the strong magnet block and the magnetic force of the constant-temperature water bath rocking bed.

Wherein, the upper part of the bag body of the digestion bag 4 is provided with two sample inlets and outlets, one is a digestive juice sample inlet and outlet 8, and the other sample inlet and outlet is integrally in the shape of a branch and comprises a main sample inlet and outlet 9 and two branch sample inlets and outlets 10. The main sample inlet and outlet 9 is used for extracting digested single cell suspension, and the left and right branch sample inlet and outlet 10 are respectively used for adding a mesenchymal stem cell serum-free complete culture medium into the bag body and discharging upper layer grease, undigested complete adipose tissues and redundant solution.

Wherein, a filter device 11 is arranged on the main sample inlet and outlet 9.

Wherein, the two side edges of the first cleaning bag 1, the second cleaning bag 2, the third cleaning bag 3 and the digestion bag 4 are respectively embedded with a shaping metal strip 12.

Wherein, but every the tip of business turn over appearance mouth all is equipped with twist-off formula sealing rubber ring 13, and every the inside interface 14 of business turn over appearance mouth and the nipple interface looks adaptation of syringe.

Wherein, every the business turn over appearance mouth through water conservancy diversion hose 5 with the internal space of the bag of first washing bag 1, second washing bag 2, third washing bag 3 and digestion bag 4 is connected.

Wherein the adipose tissue active agent 6 comprises the following components in percentage by mass: 5500.5-1% of hydroxyethyl starch, 02-0.4% of trehalose, 0.8-1.2% of human serum albumin, 0.1% of tribasic sodium citrate and the balance of PBS buffer solution.

Scales 15 are arranged on the surfaces of the first cleaning bag 1, the second cleaning bag 2, the third cleaning bag 3 and the digestion bag 4.

The diversion hose 5 and the bag body are made of transparent PVC with high resistance, high toughness, heat sealability and strong hydrophobicity and oleophobicity.

The internal shapes of the bag bodies of the three cleaning bags are rectangles with the upper ends protruding outwards and reduced, so that fat tissues can be conveniently transferred from the diversion hose through clamping after the fat is cleaned and centrifuged in the bag bodies; and the internal shape of the digestion bag body is circular, which is beneficial to the regular flow of the internal solution of the digestion bag body when the digestion bag body is vibrated in a water bath shaker, and the uniformity of the digestion degree of the adipose tissues is ensured.

The utility model discloses well first business turn over appearance mouth 16 is used for pouring into adipose tissue into, and second business turn over appearance mouth 17, third business turn over appearance mouth 18 and fourth business turn over appearance mouth 19 all are used for pouring into the washing liquid that equals with adipose tissue.

The switch of all the diversion hoses in the utility model can be controlled by the liquid stopping clamp 20.

The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the technical scope of the present invention, and the technical scope of the present invention is also considered to fall into the scope of the claims.

Claims

1. The utility model provides a many even pocket type adipose stem cell extraction element, its characterized in that, including first washing bag (1), second washing bag (2), third washing bag (3) and digestion bag (4) that connect gradually, first washing bag (1), second washing bag (2), third washing bag (3) and digestion bag (4) between communicate each other through water conservancy diversion hose (5), every wash the bag with all be equipped with at least one sample mouth of business turn over on the bag body of digestion bag (4) first washing bag (1) in advance has added adipose tissue active agent (6).

2. The multiple bag type adipose-derived stem cell extraction device according to claim 1, wherein the bag body of the digestion bag (4) is a rounded rectangle, a plurality of strong magnet attachment points (7) are provided on the bag body, the bag body is provided with strong magnet blocks through the strong magnet attachment points (7), and the bag body is fixed on the constant temperature water bath rocking bed by the magnetic force of the strong magnet blocks and the constant temperature water bath rocking bed.

3. The multi-bag type adipose-derived stem cell extraction device according to claim 2, wherein two sample inlets and outlets are arranged at the upper part of the bag body of the digestion bag (4), one sample inlet and outlet is a digestive juice sample inlet and outlet (8), and the other sample inlet and outlet is integrally in a branch shape and comprises a main sample inlet and outlet (9) and two branch sample inlets and outlets (10).

4. The multi-bag type adipose-derived stem cell extraction device according to claim 3, wherein a filtering device (11) is provided on the main inlet and outlet sample port (9).

5. The extraction apparatus of adipose-derived stem cells with multiple connected bags according to claim 1, wherein the edges of the two sides of the first cleaning bag (1), the second cleaning bag (2), the third cleaning bag (3) and the digestion bag (4) are embedded with a plastic metal strip (12).

6. The extraction apparatus of adipose-derived stem cells in multiple connected bags according to claim 1, wherein the end of each inlet/outlet sample port is provided with a twist-off sealing rubber ring (13), and the inner interface (14) of each inlet/outlet sample port is adapted to the nipple interface of a syringe.

7. The multiple connected bag type adipose-derived stem cell extraction device of claim 1, wherein each of the sample inlet and outlet ports is connected with the inner spaces of the first cleaning bag (1), the second cleaning bag (2), the third cleaning bag (3) and the digestion bag (4) through a guide hose (5).

8. The extraction device of the multi-connected bag type adipose-derived stem cells according to claim 1, wherein the adipose tissue active agent (6) comprises the following components in percentage by mass: 5500.5-1% of hydroxyethyl starch, 02-0.4% of trehalose, 0.8-1.2% of human serum albumin, 0.1% of tribasic sodium citrate and the balance of PBS buffer solution.

9. The multi-connected bag type adipose-derived stem cell extraction device according to claim 1, wherein the bag body surfaces of the first cleaning bag (1), the second cleaning bag (2), the third cleaning bag (3) and the digestion bag (4) are provided with scales (15).

10. The multiple bag type adipose-derived stem cell extraction device of any one of claims 1 to 9, wherein the material of the diversion hose (5) and the bag body is transparent PVC with high resistance, high toughness, heat sealability and strong water and oil repellency.