CN212829844U - Fat-soluble vitamin detection kit - Google Patents
Fat-soluble vitamin detection kit Download PDFInfo
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- CN212829844U CN212829844U CN202021013743.XU CN202021013743U CN212829844U CN 212829844 U CN212829844 U CN 212829844U CN 202021013743 U CN202021013743 U CN 202021013743U CN 212829844 U CN212829844 U CN 212829844U
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Abstract
The utility model discloses a fat-soluble vitamin detection kit, including kit body, buffer board, buffer package A module, buffer package B module, fat-soluble vitamin protein precipitant bottle, fat-soluble vitamin extraction liquid bottle, fat-soluble vitamin mobile phase A bottle, fat-soluble vitamin mobile phase B bottle, fat-soluble vitamin redissolution bottle, fat-soluble vitamin calibrator A ~ G pipe, interior standard substance A pipe, interior standard substance solution B pipe, quality control article QC1 pipe, quality control article QC2 pipe, centrifuging tube, silica gel pad and micropore reaction plate; through optimizing kit overall arrangement, the structure is more reasonable, and the reagent is put in order, when providing better cushioning effect for each reagent, material, the fixity is better, leads to in disorder, spill of reagent in avoiding the transportation, and detection reagent is more stable, can hold the detection reagent who detects multiple fat-soluble vitamin simultaneously, can practice thrift the parking space for producer, user, reduces the commodity circulation cost.
Description
Technical Field
The utility model relates to a detect reagent box, especially a fat-soluble vitamin detect reagent box.
Background
The world health organization refers to a deficiency of vitamins in the diet as "recessive hunger". The universal construction of the academy of Chinese academy of engineering indicates that about 20 hundred million people suffer from 'hidden hunger', the population number of 'hidden hunger' in China is up to 3 hundred million, so that reasonable vitamin supplement and monitoring level is very important for maintaining human health, and the development of a determination kit for simultaneously detecting multiple fat-soluble vitamins is imminent to assist clinical guidance of the people to effectively and reasonably supplement vitamins, achieve early discovery, early intervention and early treatment, and have great significance for reduction of nutritional diseases.
The current clinical or laboratory diagnostic methods are mainly as follows: immunochromatography, enzyme-linked immunoassay, chemiluminescence, and high performance liquid chromatography. The existing kit for detecting fat-soluble vitamins can only detect single fat-soluble vitamin, and when a plurality of fat-soluble vitamins need to be detected simultaneously, the detection time is long, the operation is complex, the cost is high, the kit is packaged more and greatly, and the storage space and the logistics cost of manufacturers and users are increased; in addition, because the inherent property of vitamin is easily decomposed by heat when being exposed to light, most of the existing kits have the problem of poor stability.
SUMMERY OF THE UTILITY MODEL
Based on this, the utility model discloses an overcome the weak point of above-mentioned prior art and provide a fat-soluble vitamin detect reagent box, the kit includes kit body, buffer board, buffer package A module, buffer package B module, fat-soluble vitamin protein precipitant bottle, fat-soluble vitamin extraction liquid bottle, fat-soluble vitamin mobile phase A bottle, fat-soluble vitamin mobile phase B bottle, fat-soluble vitamin redissolution bottle, fat-soluble vitamin calibrator A ~ G pipe, interior standard substance A pipe, interior standard substance solution B pipe, quality control article QC1 pipe, quality control article QC2 pipe, centrifuging tube, silica gel pad and micropore reaction plate;
the buffer package A module is in a step shape and is arranged at the bottom of the kit body, and the inner diameter of the bottom of the buffer package A module is consistent with that of the bottom of the kit body; the higher end of the buffer packaging module A is respectively provided with a plurality of cylindrical grooves perpendicular to the bottom, fat-soluble vitamin protein precipitator bottles, fat-soluble vitamin extraction liquid bottles, fat-soluble vitamin mobile phase A bottles, fat-soluble vitamin mobile phase B bottles and fat-soluble vitamin redissolution bottles are respectively embedded into the cylindrical grooves, the aperture of each cylindrical groove is not smaller than the diameter of each bottle, and the depth of each cylindrical groove is not smaller than the height of each reagent bottle;
the lower end of the buffer package A module is provided with a buffer package B module, a centrifuge tube, a silica gel pad and a micropore reaction plate; wherein, the centrifuge tube and the micropore reaction plate are arranged at the bottom, the buffer package B module is stacked on the centrifuge tube, and the silica gel pad is stacked on the micropore reaction plate;
a plurality of cuboid grooves are formed in the buffer packaging B module, long axes of the cuboid grooves are parallel to the bottom of the kit body, and fat-soluble vitamin calibrator A-G tubes, inner standard A tubes, inner standard dissolving liquid B tubes, quality control QC1 tubes and quality control QC2 tubes are respectively embedded in the cuboid grooves; the depth of the cuboid groove is not less than the diameter of each tube, and the length of the cuboid groove is not less than the height of each tube;
the total height of the buffer package A module at the shorter end is not higher than that of the buffer package A module at the higher end after the fat-soluble vitamin calibrator A-G tube, the inner standard A tube, the inner standard dissolving liquid B tube, the calibrator, the quality control QC1 tube and the quality control QC2 tube are stacked;
the buffer board is a cuboid flat board and is arranged above the buffer packaging A module, the size of the buffer board is consistent with the inner diameter of the top of the kit body, and the height of the top of the buffer board is the same as that of the top of the kit body.
All reagents and materials are orderly arranged in the kit, the buffer model can provide good buffer and positioning for all reagent materials, the tidiness of the kit is kept, and the accidental spilling of the reagents caused by the sliding of the reagents in the transportation process can be avoided. The internal standard A tube is internally filled with a multi-fat-soluble vitamin internal standard mixture, so that the content of the multi-fat-soluble vitamin can be detected simultaneously, and the internal standard is in a freeze-dried state and has more stable properties.
As a preferred embodiment of the kit of the present invention, the kit further comprises a centrifuge tube cassette; the centrifuge tube box is a cuboid, and the centrifuge tube is arranged in the centrifuge tube box.
General centrifuging tube packs through the sealing bag, and its shape is unset, further places in the centrifuging tube box through the sealing bag that will be equipped with the centrifuging tube, can provide better protection for the centrifuging tube on the one hand, avoids leading to the centrifuging tube effluvium because of the sealing bag sack does not seal, and on the other hand kit is cleaner and tidier. The centrifugal tube box and the micropore reaction plate are arranged on the shorter end of the buffer packaging A module in parallel, and no obvious gap exists between the centrifugal tube box and the kit body.
As the preferred embodiment of the kit, the cylinder recess on the buffer packaging A module is arranged in two lines.
As the preferred embodiment of the kit, the cuboid groove on the buffer package B module is arranged in two rows and six columns.
In a preferred embodiment of the kit of the present invention, the microwell reaction plate is a 96-well plate with a V-shaped bottom.
As the preferred embodiment of the kit of the utility model, 96 pipe position grooves are arranged on the silica gel pad.
As the preferable embodiment of the kit, the materials of the buffer board, the buffer package A module and the buffer package B module are all pearl cotton.
The pearl cotton can provide good buffering effect for each reagent and material in the kit, has good plasticity, can prepare proper embedded holes and grooves according to the sizes of different reagents, and provides good positioning while providing good buffering effect for the reagents.
As the preferable embodiment of the kit of the present invention, the bottle of the fat-soluble vitamin protein precipitant, the bottle of the fat-soluble vitamin extract solution, the bottle of the fat-soluble vitamin mobile phase a, the bottle of the fat-soluble vitamin mobile phase B, and the bottle of the fat-soluble vitamin redissolution are brown glass bottles.
Because the related reagent is easily decomposed by heat when exposed to light, the brown glass bottle can be effectively protected from light, the stability of the reagent is improved, the accuracy and the effectiveness of detection are further ensured, and the service life of the reagent is prolonged.
As a preferred embodiment of the kit of the present invention, the lipid-soluble vitamin calibrator a-G tube, the inner standard a tube, the inner standard solution B tube, the quality control QC1 tube, and the quality control QC2 tube are distinguished by bottle caps of different colors; wherein, the bottle caps of the A to G bottle of the fat-soluble vitamin calibrator have the same color; the inner standard substance A tube, the inner standard substance dissolving liquid B tube, the quality control substance QC1 tube and the quality control substance QC2 bottle caps are different in color.
The calibration material, the quality control material, the internal standard material and the like are respectively dissolved before the experiment is carried out, and the bottle caps with different colors are used, so that the user can be helped to effectively distinguish the components of each tube, and the probability of reagent misuse in the using process is reduced.
The reagent box has the advantages that the reagent box is standard, reasonable and safe in package, the whole layout of the reagent box is optimized, the structure is more reasonable, the reagents are placed in order, better buffering effect is provided for each reagent and material, the fixity is better, and the reagents are prevented from being scattered and spilled out in the transportation process; the utility model discloses the kit can hold the detect reagent who detects multiple fat-soluble vitamin simultaneously, easy operation, quick, detect the flux height, and detect reagent is more stable, detects that repeatability is good, practices thrift the parking space for producer, user, reduces the commodity circulation cost.
Drawings
FIG. 1 is a schematic diagram of the structure of a fat-soluble vitamin detection kit according to an embodiment;
FIG. 2 is a schematic structural diagram of a buffer packaging B module according to an embodiment;
the kit comprises a kit body 1, a kit body 2, a buffer plate 3, a buffer package A module, a buffer package B module 4, a fat-soluble vitamin protein precipitator bottle 5, a fat-soluble vitamin extraction liquid bottle 6, a fat-soluble vitamin extraction liquid bottle 7, a fat-soluble vitamin redissolution bottle 8, a fat-soluble vitamin mobile phase A bottle 9, a fat-soluble vitamin mobile phase B bottle 10-16, fat-soluble vitamin calibrator A-G tubes 17, inner standard A tubes 18, inner standard dissolving liquid B tubes 19, quality control QC1 tubes 20, quality control QC2 tubes 21, centrifuge tubes 22, a silica gel pad 23 and a micropore reaction plate.
Detailed Description
For better illustrating the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the accompanying drawings and specific embodiments.
Examples
An embodiment of fat-soluble vitamin detect reagent box, the schematic structure is shown in fig. 1, 2, the kit includes kit body 1, buffer board 2, buffer package A module 3, buffer package B module 4, fat-soluble vitamin protein precipitant bottle 5, fat-soluble vitamin extract bottle 6, fat-soluble vitamin redissolution bottle 7, fat-soluble vitamin mobile phase A bottle 8, fat-soluble vitamin mobile phase B bottle 9, fat-soluble vitamin calibrator A ~ G pipe 10~16, interior standard substance A pipe 17, interior standard substance solution B pipe 18, quality control article QC1 pipe 19, quality control article QC2 pipe 20, centrifuging tube 21, silica gel pad 22 and micropore reaction plate 23;
the buffer package A module 3 is in a step shape and is arranged at the bottom of the kit body 1, and the inner diameter of the bottom of the buffer package A module 3 is consistent with that of the bottom of the kit body 1; the higher end of the buffer package A module 3 is respectively provided with a plurality of cylindrical grooves vertical to the bottom, fat-soluble vitamin protein precipitator bottles 5, fat-soluble vitamin extract bottles 6, fat-soluble vitamin re-dissolving solution bottles 7, fat-soluble vitamin mobile phase A bottles 8 and fat-soluble vitamin mobile phase B bottles 9 are respectively embedded in the cylindrical grooves, the aperture of each cylindrical groove is not smaller than the diameter of each bottle, and the depth of each cylindrical groove is not smaller than the height of each reagent bottle; the cylindrical grooves on the module A3 of the buffer package are arranged in two rows; wherein the fat-soluble vitamin mobile phase A bottle 8 and the fat-soluble vitamin mobile phase B bottle 9 are positioned on the same row; the fat-soluble vitamin protein precipitator bottle 5, the fat-soluble vitamin extract bottle 6 and the fat-soluble vitamin re-dissolving liquid bottle 7 are positioned on the same line.
A buffer package B module 4, a centrifuge tube 21, a silica gel pad 22 and a micropore reaction plate 23 are arranged at the shorter end of the buffer package A module 3; wherein, the centrifuge tube 21 and the micropore reaction plate 23 are arranged at the bottom, the buffer package B module 4 is stacked on the centrifuge tube 21, and the silica gel pad 22 is stacked on the micropore reaction plate 23; the micropore reaction plate 23 is a 96-hole plate with a V-shaped bottom; each pore volume was 1.5 mL; the silica gel pad 22 is provided with 96 pipe position grooves. The kit further comprises a centrifuge tube cassette; the centrifuge tube box is a cuboid, and the centrifuge tube is arranged in the centrifuge tube box. The centrifuge tube box and the micropore reaction plate 23 are arranged on the shorter end of the buffer packaging A module 3 in parallel, and no obvious gap exists between the centrifuge tube box and the kit body 1.
A plurality of cuboid grooves are formed in the buffer packaging B module 4, long axes of the cuboid grooves are parallel to the bottom of the kit body 1, and fat-soluble vitamin calibrator A-G tubes 10-16, an inner standard A tube 17, an inner standard dissolving liquid B tube 18, a quality control QC1 tube 19 and a quality control QC2 tube 20 are respectively embedded in the cuboid grooves; the depth of the cuboid groove is not less than the diameter of each tube, and the length of the cuboid groove is not less than the height of each tube; the cuboid grooves on the buffer package B module 4 are arranged in two rows and six columns.
The total height of the buffer package A module 3 with the shorter end stacked with the fat-soluble vitamin calibrator A-G tubes 10-16, the inner standard A tube 17, the inner standard solution B tube 18, the quality control QC1 tube 19 and the quality control QC2 tube 20 is not higher than the higher end of the buffer package A module 3.
The buffer plate 2 is a cuboid flat plate and is arranged above the buffer packaging A module 3, and the size of the buffer plate 2 is consistent with the inner diameter of the top of the kit body 1; the top of the buffer plate 2 is as high as the top of the reagent kit body 1.
The buffer plate 2, the buffer package A module 3 and the buffer package B module 4 are all made of pearl wool.
The fat-soluble vitamin protein precipitator bottle 5, the fat-soluble vitamin extract bottle 6, the fat-soluble vitamin re-dissolving liquid bottle 7, the fat-soluble vitamin mobile phase A bottle 8 and the fat-soluble vitamin mobile phase B bottle 9 are brown glass bottles.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention can be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention.
Claims (9)
1. A fat-soluble vitamin detection kit is characterized by comprising a kit body (1), a buffer plate (2), a buffer package A module (3), a buffer package B module (4), a fat-soluble vitamin protein precipitator bottle (5), a fat-soluble vitamin extraction liquid bottle (6), a fat-soluble vitamin redissolution bottle (7), a fat-soluble vitamin mobile phase A bottle (8), a fat-soluble vitamin mobile phase B bottle (9), fat-soluble vitamin calibrator A-G tubes (10-16), an inner standard A tube (17), an inner standard dissolvent B tube (18), a quality control QC1 tube (19), a quality control QC2 tube (20), a centrifuge tube (21), a silica gel pad (22) and a micropore reaction plate (23);
the buffer package A module (3) is in a step shape and is arranged at the bottom of the kit body (1), and the inner diameter of the bottom of the buffer package A module (3) is consistent with that of the bottom of the kit body (1); the higher end of the buffer package A module (3) is respectively provided with a plurality of cylindrical grooves perpendicular to the bottom, fat-soluble vitamin protein precipitator bottles (5), fat-soluble vitamin extraction liquid bottles (6), fat-soluble vitamin redissolution bottles (7), fat-soluble vitamin mobile phase A bottles (8) and fat-soluble vitamin mobile phase B bottles (9) are respectively embedded in the cylindrical grooves, the aperture of each cylindrical groove is not smaller than the diameter of each bottle, and the depth of each cylindrical groove is not smaller than the height of each reagent bottle;
the lower end of the buffer package A module (3) is provided with a buffer package B module (4), a centrifuge tube (21), a silica gel pad (22) and a micropore reaction plate (23); wherein the centrifuge tube (21) and the micropore reaction plate (23) are arranged at the bottom, the buffer package B module (4) is stacked on the centrifuge tube (21), and the silica gel pad (22) is stacked on the micropore reaction plate (23);
a plurality of cuboid grooves are formed in the calibrator and the buffer package B module (4), long axes of the cuboid grooves are parallel to the bottom of the kit body (1), and fat-soluble vitamin calibrator A-G tubes (10-16), inner standard A tubes (17), inner standard solution B tubes (18), quality control QC1 tubes (19) and quality control QC2 tubes (20) are respectively embedded in the cuboid grooves; the depth of the cuboid groove is not less than the diameter of each tube, and the length of the cuboid groove is not less than the height of each tube;
the total height of the buffer package A module (3) with the shorter end stacked with the fat-soluble vitamin calibrator A-G tubes (10-16), the inner standard A tube (17), the inner standard solution B tube (18), the quality control QC1 tube (19) and the quality control QC2 tube (20) is not higher than the higher end of the buffer package A module (3);
the buffer plate (2) is a cuboid flat plate and is arranged above the buffer packaging A module (3), and the size of the buffer plate (2) is consistent with the inner diameter of the top of the kit body (1); the top of the buffer plate (2) is as high as the top of the kit body (1).
2. The kit of claim 1, further comprising a centrifuge tube cassette; the centrifuge tube box is a cuboid, and the centrifuge tube is arranged in the centrifuge tube box.
3. The kit according to claim 1 or 2, characterized in that the cylindrical recesses on the module a of the cushioning packaging (3) are arranged in two rows.
4. The kit according to claim 1 or 2, wherein the cuboid grooves on the B-module (4) of the buffer packaging are arranged in two rows and six columns.
5. The kit according to claim 1 or 2, wherein the microwell reaction plate (23) is a 96-well plate having a V-shaped bottom.
6. The kit according to claim 1 or 2, characterized in that the silicone pad (22) is provided with 96 tube site slots.
7. The kit according to claim 1 or 2, wherein the materials of the buffer plate (2), the buffer package A module (3) and the buffer package B module (4) are all pearl cotton.
8. The kit according to claim 1 or 2, wherein the fat-soluble vitamin protein precipitant bottle (5), the fat-soluble vitamin extract solution bottle (6), the fat-soluble vitamin redissolution bottle (7), the fat-soluble vitamin mobile phase A bottle (8) and the fat-soluble vitamin mobile phase B bottle (9) are brown glass bottles.
9. The kit according to claim 1 or 2, characterized in that the lipid-soluble vitamin calibrator A-G tube (10-16), the internal standard A tube (17), the internal standard solution B tube (18), the quality control QC1 tube (19), and the quality control QC2 tube (20) are distinguished by bottle caps of different colors; wherein the bottle caps of the A-G tubes (10-16) of the fat-soluble vitamin calibrator have the same color; the colors of the inner standard substance A tube (17), the inner standard substance dissolving liquid B tube (18), the quality control substance QC1 tube (19) and the quality control substance QC2 tube (20) are different.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021013743.XU CN212829844U (en) | 2020-06-04 | 2020-06-04 | Fat-soluble vitamin detection kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021013743.XU CN212829844U (en) | 2020-06-04 | 2020-06-04 | Fat-soluble vitamin detection kit |
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| CN212829844U true CN212829844U (en) | 2021-03-30 |
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| CN202021013743.XU Active CN212829844U (en) | 2020-06-04 | 2020-06-04 | Fat-soluble vitamin detection kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111676212A (en) * | 2020-06-15 | 2020-09-18 | 深圳市瑞赛生物技术有限公司 | Culture method and kit for quantitatively detecting vitamins by microorganisms |
-
2020
- 2020-06-04 CN CN202021013743.XU patent/CN212829844U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111676212A (en) * | 2020-06-15 | 2020-09-18 | 深圳市瑞赛生物技术有限公司 | Culture method and kit for quantitatively detecting vitamins by microorganisms |
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Address after: 510000 No. 6, Yinyi street, economic and Technological Development Zone, Guangzhou, Guangdong Patentee after: Guangzhou Fenghua Biological Co.,Ltd. Address before: No.6, Yinyi street, development zone, Guangzhou, Guangdong 510000 Patentee before: GUANGZHOU FENGHUA BIOENGINEERING Co.,Ltd. |
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