CN210051772U

CN210051772U - Kit for rapidly detecting phenylethanolamine A in animal derived food

Info

Publication number: CN210051772U
Application number: CN201920241959.2U
Authority: CN
Inventors: 吴小平
Original assignee: Beijing Cheerkang Biotechnology Co Ltd
Current assignee: Beijing Cheerkang Biotechnology Co Ltd
Priority date: 2019-02-26
Filing date: 2019-02-26
Publication date: 2020-02-11
Anticipated expiration: 2029-02-26

Abstract

The utility model discloses a phenylethanolamine A kit in short-term test animal derived materials food, including the standing groove of arranging reagent frame and trace test orifice plate in the box body in, the standing groove sets up one side of reagent frame, be equipped with standard liquid bottle groove, high concentration standard article bottle groove, substrate liquid bottle groove, termination liquid bottle groove, enzyme marker thing bottle groove, antibody working solution bottle groove and concentrated washing liquid bottle groove on the reagent frame, the trace test orifice plate comprises orifice plate frame and orifice plate strip. The utility model discloses the experiment shows that this kit has very high sensitivity, and is easy and simple to handle, detects fast, accurate, sensitive, stable, and operating personnel technical content requires not highly, can carry out the survey of phenylethanolamine A content in a plurality of samples in succession, has reduced the required time of inspection sample, and the cost is lower.

Description

Kit for rapidly detecting phenylethanolamine A in animal derived food

Technical Field

The utility model relates to a compound detects technical field, in particular to phenylethanolamine A kit in short-term test animal derived food.

Background

Phenylethanolamine a, also known as krusemame, has the molecular formula: c ₁₉H ₂₄N ₂O ₄It belongs to β -stimulant, has good effect, high economic return and quick response, and can improve the ratio of meat to fat of fat animals and can improve the fat-fat ratio of fat animalsAccelerate the growth of animals, and is widely added into animal feed and can remain in the bodies of the animals. However, this drug has serious side effects on humans. The accumulated intake dose of people exceeds a certain value, the patients with mild symptoms are poisoned, the patients with severe symptoms are heart diseases, and other poisoning symptoms comprise tachycardia, arrhythmia, diarrhea, muscle pain, nausea, dizziness and the like, so the use of the traditional Chinese medicine is forbidden. It is imminent to detect the residual amount of phenylethanolamine a in foods of animal origin.

In terms of detection methods of phenylethanolamine a, currently, the most commonly used methods mainly include methods such as High Performance Liquid Chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), and enzyme-linked immunosorbent assay (ELISA). The HPLC method has the advantages of high detection accuracy and low false positive rate, and has the main defects of high instrument price, more complicated operation, long time consumption and high detection cost; and the selection of the extraction conditions of the early samples also has great influence on the detection sensitivity and accuracy. The GC-MS has high sensitivity and low false positive rate. The main disadvantage of this method is the complex pre-treatment of the sample with derivatization. LC-MS has the same disadvantages as GC-MS in that it requires complicated procedures and expensive instruments, and thus is generally used as a means of confirming a positive result. The enzyme-linked immunosorbent assay is one of the most widely applied and developed immunoenzyme technologies at present, and has the main advantages of rapid detection, simple sample pretreatment, simple operation of a detection system, low cost and convenience for large-scale detection.

SUMMERY OF THE UTILITY MODEL

The utility model provides a phenylethanolamine A kit in short-term test animal derived nature food solves the problem that the operation is more loaded down with trivial details, and is long consuming time, and the testing cost is expensive in the current phenylethanolamine A detects.

In order to solve the technical problem, the utility model adopts the technical scheme that:

the utility model provides a phenylethanolamine A kit in short-term test animal derived from nature food, including the standing groove of arranging reagent frame and trace test orifice plate in the box body in, the standing groove sets up one side of reagent frame, be equipped with standard liquid bottle groove, high concentration standard article bottle groove, substrate liquid bottle groove, termination liquid bottle groove, enzyme marker thing bottle groove, antibody working solution bottle groove and concentrated washing liquid bottle groove on the reagent frame, the trace test orifice plate comprises orifice plate frame and orifice plate strip.

Wherein, preferably, the number of the standard liquid tanks is 6, and respectively placed with 0ppb standard liquid bottles of 1 m/bottle, 0.1ppb standard liquid bottles of 1 m/bottle, 0.3ppb standard liquid bottles of 1 m/bottle, 0.9ppb standard liquid bottles of 1 m/bottle and 2.7ppb standard liquid bottles of 1 m/bottle.

Wherein, preferably, 1ml of high concentration standard bottle is placed in the high concentration standard bottle groove.

Wherein, preferably, the number of the substrate solution bottle grooves is 2, and a 7ml substrate A solution bottle with a brown cap and a 7ml substrate B solution bottle with a black cap are respectively placed in the substrate solution bottle grooves.

Wherein, preferably, a 7ml stop solution bottle with a yellow cap is placed in the stop solution bottle groove.

Wherein, preferably, a 7ml enzyme label bottle with a red cap is arranged in the enzyme label bottle groove.

Wherein, preferably, a 7ml antibody working solution bottle with a green cap is placed in the antibody working solution bottle groove.

Wherein, preferably, a 40ml concentrated washing liquid bottle with a white cap is arranged in the concentrated washing liquid bottle groove.

Wherein, preferably, the number of the pore plate strips is 12, and each pore plate strip is provided with 8 micropores pre-coated with the coupled phenylethanolamine A antigen.

The utility model has the advantages that:

experiments show that the kit has the advantages of high sensitivity, simple and convenient operation, quick, accurate, sensitive and stable detection, low requirement on technical content of operators, capability of continuously measuring the content of the phenylethanolamine A in a plurality of samples at one time, reduction of the time required for detecting the samples and low cost.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural view of a reagent rack and a placing groove in the present invention;

fig. 2 is a schematic structural view of the medium and micro test orifice plate of the present invention.

In the figure, 1, a standard solution bottle groove, 2, a high-concentration standard product bottle groove, 3, a substrate solution A bottle groove, 4, a substrate solution B bottle groove, 5, a stop solution bottle groove, 6, an enzyme marker bottle groove, 7, an antibody working solution bottle groove, 8, a concentrated washing solution bottle groove, 9, a placing groove, 10, a reagent rack, 11, a pore plate rack, 12, a pore plate strip

Detailed Description

The technical solution of the present invention will be described clearly and completely below with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1 and fig. 2, the embodiment provides a kit for rapidly detecting phenylethanolamine a in animal-derived food, including a reagent rack 10 and a placing groove 9 of a micro-scale test pore plate, which are arranged in a box body, the placing groove 9 is arranged on one side of the reagent rack 10, the reagent rack 10 is provided with a standard solution bottle groove 1, a high-concentration standard product bottle groove 2, a substrate solution bottle groove, a stop solution bottle groove 5, an enzyme-labeled object bottle groove 6, an antibody working solution bottle groove 7 and a concentrated washing solution bottle groove 8, and the micro-scale test pore plate is composed of a pore plate rack 11 and a pore plate strip 12.

Wherein, the number of the standard liquid tanks is 6, and respectively placed with 0ppb standard liquid bottles of 1 m/bottle, 0.1ppb standard liquid bottles of 1 m/bottle, 0.3ppb standard liquid bottles of 1 m/bottle, 0.9ppb standard liquid bottles of 1 m/bottle and 2.7ppb standard liquid bottles of 1 m/bottle.

Wherein, a high-concentration standard bottle of 1ml is arranged in the high-concentration standard bottle groove 2.

Wherein, the number of the substrate liquid bottle grooves is 2, which are respectively a substrate liquid A bottle groove 3 and a substrate liquid B bottle groove 4, and 7ml of substrate liquid A bottles with brown caps and 7ml of substrate liquid B bottles with black caps are respectively placed.

Wherein, a 7ml stop solution bottle with a yellow cap is arranged in the stop solution bottle groove 5.

Wherein, a 7ml enzyme label bottle with a red cap is arranged in the enzyme label bottle groove 6.

Wherein, a 7ml antibody working solution bottle with a green cap is arranged in the antibody working solution bottle groove 7.

Wherein, a 40ml concentrated washing liquid bottle with a white cap is arranged in the concentrated washing liquid bottle groove 8.

Wherein, the number of the pore plate strips is 12, each pore plate strip is provided with 8 micropores pre-coated with the coupled phenylethanolamine A antigen, namely, a 96-pore micro-test pore is arranged on the micro-test pore plate. A96-hole micro-test hole is used as a solid phase carrier and is placed in an aluminum foil bag, phenylethanolamine A coupling antigen is pre-coated in the micro-test hole to prepare a test board, and a plastic hard film is used as a cover board film.

Then 40ml of concentrated washing liquid, 7ml of enzyme marker, 7ml of phenylethanolamine A antibody working solution, 7ml of substrate A solution, 7ml of substrate B solution and 1ml of high-concentration standard substance (100ppb), wherein reagents are packaged by plastic bottles and glass bottles with different sizes and colors and are fixed in a foam mould to be packaged in a box together with the trace test pore plate frame 11 and the cover plate film so as to be convenient to carry and transport.

The reagents in each kit are sufficient for 96 assays (including standard assay wells), either for one continuous multiple sample assay or for multiple plate wells that are detached. During detection, the residue (phenylethanolamine A) in the sample competes with the coupled phenylethanolamine A antigen pre-coated on the plate of the enzyme-labeled plate hole for the phenylethanolamine A antibody, the enzyme-labeled substance is added, the TMB substrate is used for color development, the absorbance value of the sample is in negative correlation with the content of the residue (phenylethanolamine A) in the sample, the absorbance value is compared with a standard curve and then multiplied by the corresponding dilution multiple, the residual amount of the phenylethanolamine A in the sample can be obtained, and the operation is simple and easy.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A kit for rapidly detecting phenylethanolamine A in animal derived food is characterized in that: including the standing groove of arranging reagent frame and the micro-test orifice plate in the box body, the standing groove sets up one side of reagent frame, be equipped with standard liquid bottle groove, high concentration standard article bottle groove, substrate liquid bottle groove, termination liquid bottle groove, enzyme marker thing bottle groove, antibody working solution bottle groove and concentrated washing liquid bottle groove on the reagent frame, the micro-test orifice plate comprises orifice plate frame and orifice plate strip.

2. The kit for rapidly detecting phenylethanolamine A in animal derived food according to claim 1, wherein: the number of the standard liquid tanks is 6, and 0ppb standard liquid bottles of 1 m/bottle, 0.1ppb standard liquid bottles of 1 m/bottle, 0.3ppb standard liquid bottles of 1 m/bottle, 0.9ppb standard liquid bottles of 1 m/bottle and 2.7ppb standard liquid bottles of 1 m/bottle are respectively arranged in the standard liquid tanks.

3. The kit for rapidly detecting phenylethanolamine A in animal derived food according to claim 1, wherein: and a 1ml high-concentration standard bottle is placed in the high-concentration standard bottle groove.

4. The kit for rapidly detecting phenylethanolamine A in animal derived food according to claim 1, wherein: the number of the substrate liquid bottle grooves is 2, and a 7ml substrate A liquid bottle with a brown cap and a 7ml substrate B liquid bottle with a black cap are respectively placed in the substrate liquid bottle grooves.

5. The kit for rapidly detecting phenylethanolamine A in animal derived food according to claim 1, wherein: and a 7ml stop solution bottle with a yellow cap is placed in the stop solution bottle groove.

6. The kit for rapidly detecting phenylethanolamine A in animal derived food according to claim 1, wherein: and a 7ml enzyme label bottle with a red cap is placed in the enzyme label bottle groove.

7. The kit for rapidly detecting phenylethanolamine A in animal derived food according to claim 1, wherein: and a 7ml antibody working solution bottle with a green cap is placed in the antibody working solution bottle groove.

8. The kit for rapidly detecting phenylethanolamine A in animal derived food according to claim 1, wherein: a40 ml concentrated washing liquid bottle with a white cap is placed in the concentrated washing liquid bottle groove.

9. The kit for rapidly detecting phenylethanolamine A in animal derived food according to claim 1, wherein: the number of the pore plate strips is 12, and each pore plate strip is provided with 8 micropores pre-coated with coupled phenylethanolamine A antigens.