CN214953563U - Kit for simultaneously detecting sodium picosulfate, bisacodyl and desacetylbisacodyl - Google Patents

Abstract

The utility model discloses a detect fast sieve kit of sodium picosulfate, bisacodyl and deacetylation bisacodyl simultaneously. The reagent bottle comprises a packaging box, wherein the packaging box comprises a test paper barrel, a reagent bottle, a sample tube, a sampling spoon and a scale suction tube, the test paper barrel contains test paper strips and micropores, the reagent bottle comprises a bottle cap and a bottle body, and a sealing ring is arranged at the joint of the bottle cap and the bottle body. The utility model discloses in concentrating all medicaments and the instrument that need to use to a packing carton, no longer need use outside main equipment, it carries and convenient to use, is favorable to detecting the on-the-spot short-term test of the quick sieve kit of picosulfate, bisacodyl and deacetylation bisacodyl in the food simultaneously.

Description

Kit for simultaneously detecting sodium picosulfate, bisacodyl and desacetylbisacodyl

Technical Field

The utility model relates to a kit, concretely relates to detect quick examination kit of sodium picosulfate, bisacodyl and deacetylation bisacodyl simultaneously.

Background

In recent years, the phenomenon of illegal addition in foods, health-care foods and Chinese patent medicines is infinite, wherein products with the effects of losing weight, slimming, relaxing the bowels and the like are declared to be serious disaster areas which are illegally added, and the infinite new illegal additives are always research hotspots in the analysis field.

Sodium picosulfate, bisacodyl, and desacetylbisacodyl are diphenylmethane stimulant laxatives that enhance intestinal motility and intestinal secretion by stimulating sensory nerve endings in the colonic mucosa. After being taken orally, sodium picosulfate and bisacodyl are hydrolyzed into active bisphenol by deacetylase or colon bacteria in intestinal tract, thereby promoting colon peristalsis, reducing transmission time, promoting accumulation of water electrolyte in colon cavity, softening feces, and increasing feces volume. The medicine is suitable for treating various constipation, is mainly used for emptying intestinal tracts before digestive organ examination or operation, has adverse reactions including gastrospasm, acute abdomen (appendicitis, gastroenteritis, rectal bleeding and intestinal obstruction), inflammatory bowel disease, anal or hemorrhoid rupture and serious water electrolyte disorder, and can not be taken for a long time. Deacetylbisacodyl is a major impurity of sodium picosulfate and bisacodyl drugs, and is often detected simultaneously in products where illegal addition acts exist.

Sodium picosulfate, bisacodyl and deacetylbisacodyl belong to novel illegal additives of weight-reducing products, and the pathogenic and lethal conditions of taking the laxative weight-reducing products containing the medicaments occasionally cause serious harm to the health of consumers, so that the sodium picosulfate, the bisacodyl and the deacetylbisacodyl are key treatment objects of the laxative weight-reducing products. At present, the related illegal addition research reports are less, and the detection methods mainly comprise high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) and High Performance Liquid Chromatography (HPLC). According to the food supplement inspection methods BJS 201701 and BJS 201911 in China, the bisacodyl and sodium picosulfate are measured by an HPLC-MS/MS method. The methods are accurate in qualitative and quantitative determination, but are complex in operation and long in detection time, field rapid detection is difficult to realize, and technical support cannot be provided for field evidence collection and law enforcement.

Disclosure of Invention

An object of the utility model is to provide a detect fast sieve kit of sodium picosulfate, bisacodyl and deacetylation bisacodyl simultaneously.

The utility model adopts the technical proposal that:

the utility model provides a detect kit of sodium picosulfate, bisacodyl and deacetylation bisacodyl simultaneously, includes the packing carton, its characterized in that, contain in the packing carton test paper bucket, reagent bottle, sample cell, sample spoon, scale straw contain test paper strip and micropore in the test paper bucket.

As a further improvement of the technical scheme, the reagent bottle comprises a bottle cap and a bottle body, and a sealing ring is arranged at the joint of the bottle cap and the bottle body.

As a further improvement of the technical scheme, the bottle cap comprises an upper cap and a lower cap, and the lower cap is arranged at one end far away from the bottle body.

As a further improvement of the technical scheme, the test paper barrel comprises a bottle cap and a barrel body, and a sealing ring is arranged at the joint of the bottle cap and the barrel body.

As a further improvement of the above technical solution, the micro-pores have micro-pore plugs.

As a further improvement of the technical scheme, the pipette comprises a 1ml graduated pipette and a 0.2ml graduated pipette.

As a further improvement of the technical scheme, be provided with the buffering collet in the packing carton, be provided with the shrinkage pool that is used for holding reagent bottle and test paper bucket on the buffering collet.

As a further improvement of the technical scheme, the reagent in the reagent bottle is Tris-PBS buffer solution.

As a further improvement of the technical scheme, the micropores contain specific antigens marked by colloidal gold.

As a further improvement of the technical scheme, the number of the reagent bottles, the micropores, the test paper strips, the sampling spoon, the sample tubes, the 1ml scale straws and the 0.2ml scale straws is 10.

The utility model has the advantages that:

(1) to the liquid sample, the utility model discloses arrange sample diluent reagent bottle in after with 1mL plastics scale straw sample 0.5mL, shake evenly for subsequent use. For solid samples, about 0.5g of solid sample is taken in a graduated stopple tube, the sample diluent is added to 10mL of the graduation line, shaken vigorously for 30s, and kept stand for 2 min. The test paper barrel is opened, and the red micropores corresponding to the number of the samples are taken out and placed on the red micropore plate frame. And opening a sample diluent reagent bottle or a bottle cap with a graduated sample tube and a plug, sucking 0.2mL of the supernatant of the sample solution into the red micropores by using a 0.2mL plastic graduated pipette, and sucking 1-3 times up and down by using the 0.2mL plastic pipette to mix uniformly. And taking out the test strips with the quantity corresponding to that of the samples from the test strip barrel, inserting the test strips into the red micropores, reacting for 3min at 20-40 ℃, taking out the test strips from the red micropores, removing a sample pad at the lower end of the test strips, and performing result interpretation.

The rapid screening kit improves the formula and proportion of the sample diluent reagent, the red micropore content and the test strip, and concentrates all the medicaments and tools which need to be used in one packaging box, does not need to use external large-scale equipment, is convenient to carry and use, and is beneficial to the on-site rapid determination of the rapid screening kit for simultaneously detecting the sodium picosulfate, the bisacodyl and the deacetylbisacodyl in food.

(2) The utility model has the advantages of the specificity is good, sensitivity is high, easy and simple to handle, can realize on-the-spot short-term test, the needs that satisfy supervision department, detection mechanism on-the-spot supervision law enforcement that can be better.

Drawings

Fig. 1 is a schematic structural diagram of the packing box of the present invention.

Fig. 2 is a schematic structural diagram of the present invention.

Fig. 3 is a schematic view illustrating the interpretation of the detection result of the present invention.

Detailed Description

Referring to fig. 1 and 2, the detailed structure of the preferred embodiment of the present invention is shown, and the structural features of the components of the present invention will be described in detail below.

As shown in fig. 1 and 2, the utility model provides a detect quick screening kit of sodium picosulfate, bisacodyl and deacetylation bisacodyl simultaneously, including packing carton 1 of leatheroid, be equipped with 1 test paper bucket 2 in the packing carton 1, 10 bottles of sample diluent reagent bottles 3, 10 utensil stopper sample tubes 5 of taking the scale, 10 sampling scoops 4, 10 1mL plastics scale straw 7 and 10 0.2mL plastics straws 6. The test paper barrel 2 is internally provided with 10 test paper strips 9 and 10 red micropores 10, and each micropore contains a specific antigen marked by colloidal gold. The sample diluent reagent bottle 3 is filled with Tris-PBS buffer solution.

Further, the minimum scale of a 1mL plastic scale pipette is 0.5 mL.

Further, the minimum scale of a 0.2mL plastic scale pipette is 0.1 mL.

Further, the reagent in the reagent bottle is Tris-PBS buffer solution.

Further, the micropores contain specific antigens marked by colloidal gold.

Further, be provided with buffering collet 8 in the packing carton 1, avoid the reagent bottle because the damage that jolts and cause in the transportation, be provided with the shrinkage pool that is used for holding sample diluent reagent bottle 3 and test paper bucket 2 on the buffering collet 8. The sample diluent reagent bottle 3 comprises a bottle cap and a bottle body, a sealing ring is arranged at the joint of the bottle cap and the bottle body, the bottle cap comprises an upper cover 10 and a lower cover 11, and the lower cover is arranged at one end far away from the bottle body. Preventing leakage of liquid during transportation. The test paper barrel 2 comprises a bottle cap 13 and a barrel body, and a sealing ring is arranged at the joint of the bottle cap 13 and the barrel body to prevent the test paper 9 from being affected with damp. The red wells 10 have a well plug to protect the specific antibodies labeled with colloidal gold therein from moisture.

The method for rapidly screening the kit for sodium picosulfate, bisacodyl and desacetylbisacodyl in food by using the kit in the embodiment comprises the following steps:

sample pretreatment

a. Liquid sample: after shaking up, 0.5mL of the sample was aspirated through a 1mL plastic graduated pipette 7, added to the sample diluent reagent bottle 3, and shaken up.

b. Solid sample: taking about 0.5g of sample in a sample tube 5 with a plug and scales, adding sample diluent to a scale mark of 10mL, shaking vigorously for 30s, and standing for 2 min.

Detection of

(1) The test paper bucket 2 is opened and the red wells 10 corresponding to the number of samples are removed.

(2) Opening the bottle cap of the sample diluent reagent bottle 3 or the graduated bottle cap with the plug sample tube 5, sucking 0.2mL of sample liquid by using a 0.2mL plastic suction tube 6, and dripping the sample liquid into the red micropore 10.

(3) Sucking up and down 1-3 times by using a 0.2mL plastic suction pipe 6.

(4) And opening the test paper barrel 2, taking out the test paper strips 9 corresponding to the number of the samples, inserting the test paper strips into the corresponding red micropores 10, and reacting for 3min at 20-40 ℃.

(6) The test strip 9 is removed from the red wells 10, the sample pad at the lower end of the test strip is removed, and the results are read.

Determination of results

(1) When the control line (line C) is normally developed, the result is judged according to the following criteria:

color depth comparison of detection line (T line) and control line (C line):

line T is greater than or equal to line C and is negative;

positive results were obtained when the T line was < C or the T line did not develop color.

(2) When the control line (C line) did not develop color, the detection result was invalid, and it was suggested to retest. The interpretation scheme is shown in fig. 3.

The above description is only a preferred embodiment of the present invention, and does not limit the scope of the present invention. Other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principles of the invention are intended to be included within the scope of the invention.

Claims (10)

1. The utility model provides a detect kit of sodium picosulfate, bisacodyl and deacetylation bisacodyl simultaneously, includes the packing carton, its characterized in that, contain test paper bucket, reagent bottle, sample cell, sampling spoon, scale straw in the packing carton, contain test paper strip and micropore in the test paper bucket.

2. The kit according to claim 1, wherein the reagent bottle comprises a bottle cap and a bottle body, and a sealing ring is arranged at the joint of the bottle cap and the bottle body.

3. The kit of claim 2, wherein the vial cap comprises an upper cap and a lower cap, the lower cap being disposed at an end distal from the vial body.

4. The kit of claim 1, wherein the test paper barrel comprises a bottle cap and a barrel body, and a sealing ring is arranged at the joint of the bottle cap and the barrel body.

5. The kit of claim 1, wherein the microwells have microwell plugs.

6. The kit of claim 1, wherein the graduated pipette comprises a 1ml graduated pipette and a 0.2ml graduated pipette.

7. The kit of claim 1, wherein a buffer base is arranged in the packaging box, and a concave hole for accommodating the reagent bottle and the test paper barrel is arranged on the buffer base.

8. The kit of claim 1, wherein the reagents in the reagent bottles are Tris-PBS buffer solution.

9. The kit of claim 1, wherein the microwells contain specific antigens labeled with colloidal gold.

10. The kit according to any one of claims 1 to 9, wherein the number of the reagent bottles, the micropores, the test strips, the sampling spoon, the sample tubes, the 1ml graduated pipettes and the 0.2ml graduated pipettes is 10.

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